Quawell - Lab Supplies Scientific
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1. lt 100ng ul 2ng ul gt 100ng ul 2 Absorbance range 0 80 A Concentration range 0 4000 ng ul dsDNA Measurement cycle time 2s Dimension 20 cm x 15cm x 12 cm Sample pedestal material stainless steel Optical fiber material quartz Operating voltage 12V Operation power consumption lt 10 W Standby power consumption lt 3W CE Ready 21 Quawell Q3000 USER MANUAL B Absorbance calculation When the Q3000 Spectrophotometer is blanked a spectrum is taken of a reference material blank and stored in memory as an array of light intensities by wavelength When a measurement of a sample is taken the intensity of light that has transmitted through the sample is recorded The sample intensities along with the blank intensities are used to calculate the sample absorbance according to the following equation I Abs tog a Lag Thus the measured light intensity of both the sample and of the blank are required to calculate the absorbance at a given wavelength 22 Quawell Q3000 USER MANUAL C Concentration Calculation Beer s Law The Beer Lambert equation is used to correlate the calculated absorbance with concentration A E b c Where A is the absorbance represented in absorbance units A E is the wavelength dependent molar absorptivity coefficient or extinction coefficient with units of liter mol cm b is the path length in cm and c is the analyte concentration in moles liter or molar2. Quawell Q3000 UV SPECTROPHOTOMETER USER MANUAL V3 1 1 Quawell ometer UV Spectrophot o Quawell Technology Inc All rights reserved Rev 08 2011 Quawell Q3000 USER MANUAL Table of Contents 1 OVERVIEW EE paer NENEA aai hadena 5 age 1062 OT EE 5 Basic operation L meosuiuieumesinntmknujvmniaaanneeduiknndeakentdsnende 5 Patents and unique technologies ccecccceeeeeeeeeeeeeeeeeeeneeeeeeeeeeeeeneeeees 6 2 INSTALLATION Pee eee nore ener eee eee nearer eee en ne te 8 Computer requirements ccceeeeeeeeccceeeeeeeeeeeeeceeeeeeeesecaeaeeeeeeeeeaeeeeees 8 SoftWare SAP Sic cera et aree nencleedsercaeeed Hen seca sig eet cetecuende ee ue 8 Install 03000 and the driver Luse cccocwunsseonnadeseewsondocmadecdaa tenets 8 HE Nese 8 3 MENU AND FUNCTIONS FOR THE APPLICATIONS rrnnrnrnnnnnnnnenn 9 ME are did 9 BURNS on the NE vaere serve seewues eee 10 pe ENE ERR E E a ae aana 10 Functions for Nucleic Acid and Protein A280 10 Making ITIGASUMGIMGIN 42 25 tecesidaeelecdcenseee chee sabes sahdeenidederendtade binned 11 4 NUCLEIC ACIDS Lises ATGE Eek 12 Nucleic acid screen featureS cccecee cece cece cence eeeeeeeeeeeeeeeees 12 SUT ONS MD EEE 12 Sample Type E G06 en2ctitencnsecteodceceerediecdcance death caeeceurecewsdtes biGeeseee 12 POS ZOU Le cue E E EE cen te eeemnens 12 ABS as 12 200 280 EEE EN EE EEE 12 NG EE ude E N 12 Quawell Q3000 USER MANUAL Measurement range araren
3. contact technical support 18 Quawell Q3000 USER MANUAL Q3000 Connection Error check USB cable and try again Sample accuracy and reproducibility If the sample data are inaccurate and or not reproducible please refer to Section 6 POTENTIAL SOURCES OF ERROR 19 Quawell Q3000 USER MANUAL 8 MAINTENANCE AND CALIBRATION Maintenance The primary maintenance requirement of the Q3000 spectrophotometer is to keep the measurement surfaces clean Upon completion of measurement to prevent sample carryover and residue buildup wipe the sample from the upper and lower measurement surfaces as well as the surrounding area with de ionized water Check the calibration Option The Diagnostic function checks the instrument calibration It is recommended that run this function every six months Quawell CR101 is required to run the Diagnostic function You can order CR101 through Quawell or your local distributor CR101 is an aqueous potassium dichromate K2Cr207 solution l d Menu Quawell a3000uv Spectrophotometer l S N ready CCE 10001000 20 Quawell Q3000 USER MANUAL 9 APPENDICES A Instrument specifications Path length 0 2 mm to 1mm auto selected Sample size requirement 1 5 ul Light source LED Detector photoelectric cell Wavelength range 260nm 280nm Wavelength accuracy lt 2 nm Absorbance precision 0 001 Abs Accuracy
4. e 2003 or higher e Adobe Reader e CPU 400MHZ or higher e 64MB RAM e USB port e Monitor 1024x768 e 500MB Hard disk available Software Setup Insert the CD into the CD ROM open the folder Q3000 double click the file Setup and follow the prompts to finish installation of the software Install Q3000 and the Driver Connect the instrument to the PC using the USB cable shipped with the Q3000 the computer will automatically find the drive and finish the installation Input CCE No For first time to use it is required to input the CCE No Note wrong CCE No input will cause the measurement error Quawell Q3000 USER MANUAL 3 MENU AND FUNCTIONS FOR THE APPLICATIONS Menu Double click the shortcut Q3000 on the desktop the menu as below will be displayed on the screen r l d Menu Buttons on the menu 1 2 Nucleic Acid the application for samples of dsDNA ssDNA and RNA Protein A280 the application for sample of pure proteins BSA IgG and Lysozyme Edit CCE No allows the users to edit CCE No when it is necessary User Manual display the user manual by click the button Change Password change the password that is required for edit CCE No Diagnostic check the calibration Quawell Q3000 USER MANUAL Password Password is required for edit CCE No The default password is admin The user can edit the password using Edit Passwo
5. ed to save it 11 Quawell Q3000 USER MANUAL 4 NUCLEIC ACIDS Nucleic acid screen features Select Nucleic Acid application from the Q3000 menu the module interface will be displayed on the screen as below l Nucleic Acid Data Table Date 8 5 2011 SampleType A260 A280 260 280 ng ul Sample ID My Sample Sample Type dsDNA e Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is My Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 12 Quawell Q3000 USER MANUAL e Sample Type Chose the sample type for the sample to be measured There are 3 different sample types for nucleic acids dsDNA ssDNA and RNA The default is dsDNA The sample type is disabled after blanking The extinction coefficient of the sample selected will appear in the next window on the right e Ads260 Absorbance of the sample at 260 nm e Abs280 Absorbance of the sample at 280 nm e 260 280 Ratio of absorbance at 260 nm and 280 nm e ng ul Sample concentration in ng ul See Appendix C for more details on this calculation Measurement range dSDNA Concentration Reproducibility Abs accuracy 1mm 1 to 4000 ng
6. he procedure for checking the calibration not available 0 20 9 APPENDICES Lee 21 Quawell Q3000 USER MANUAL A Instrument specifications rararnrnrnnnnnnnenennne cece teense eeeeeneaees 21 B Absorbance CalCUlatio Mise sadcs vitae cies tomme seu eeeeencendeus 22 C Concentration calculation Beers Law sceeeeeeeeeeeeeeees 23 D Gontaerinformatonumusumusasmsanbtnipuisiteneiriibkuivdkkei nj de 24 Quawell Q3000 USER MANUAL 1 OVERVIEW Introduction Quawell Q3000 is a micro volume UV spectrophotometer specifically designed for the measurement of nucleic acids and pure proteins Using a unique technology that holds 1 2 ul samples between the upper and lower optical surfaces without the use of a cuvette Q3000 has ability to measure the sample in less than 2 seconds with a high degree of accuracy and reproducibility Basic operation Operation of the Q3000 is simple and efficient The Q3000 uses the technologies that automatically make all necessary measurements and calculations The data is presented in the module data window and is easily exported to an Excel spreadsheet To operate 1 Lift the upper arm place 1 5ul blank buffer to the lower surface 2 Lower the upper arm and click the Blank button 3 Lift the upper arm and wipe the blank buffer from both surfaces 4 Place 1 5ul sample on the lower surface set the upper arm back to the measurement position and click the Mea
7. ignificant deviation in the data generated by any measurement system including the spectrophotometer 16 Quawell Q3000 USER MANUAL Effect of evaporation Sample evaporation could cause a 1 2 deviation in the sample s concentration Insufficient sample volume We recommend 2ul for normal sample measurements This sample size ensures that the liquid sample forms a complete column between the upper and lower optical surfaces 17 Quawell Q3000 USER MANUAL 7 TROUBLESHOOTING Q3000 driver installation error Driver Software Installation Device driver software was not successfully installed Please consult with your device manufacturer for assistance getting this device installed CP2102 USB to UART Bridge Controller Wizard canceled by user Close To solve this problem by manually install the driver as follows 1 Disconnect Q3000 2 Insert the CD into CD ROM click the driver CP210xVCPInstaller in following folder C Program Files Quawell Q3000 V3 0 1 SiLabs MCU CP210x 3 Connect Q3000 to computer with the USB cable 4 lf there is still a problem with the driver installation please contact technical support at 408 676 7626 USB connection error This error screen appears when there is a communication problem between the Q3000 and the computer It usually indicates that the Q3000 and PC connection failed To correct the error unplug the USB cable wait for 2 3 seconds and plug it again or
8. ity M 23 Quawell Q3000 USER MANUAL D Contact Information Quawell Technology Inc P O Box 612738 San Jose CA 95161 2738 USA Tel 408 329 6241 www quawell com Service and technical support Tel 408 838 3919 Note All information in this user manual is believed to be correct and complete and is subject to change without notice 24
9. nnnnnnnnnnnnnnneannnnneneananenenenennenenenennenen 13 Sample VAN Paa 13 5 PROTEIN A260 ssicscceticccatidenscecenaticeien tetra REE 14 Nucleic acid screen features ccceceeee cece eens ee eeeeeeeeeeeeeeeeeeeeeees 14 sample DE EE EE eres ere ere ae een ener er 14 OUI ON TG assess csc aides Gide Seas a idue Ee AEA 14 ME NG 14 PDS EE EE EEE 14 2600 280 a a a E ke 14 PIG EEE EEE EEE 14 Measurement NJ jens eeaeekd ean 15 Sample ODE aoveocauxtxsasnozedigsdevusszesuedsasusonedueddxansdedenkuvaoncudeeded 15 6 POTENTIAL SOURCES OF ERROR mannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 16 Sample OVEN AD ccss2ncucsssevssatuadensimeses cadedcanageseudeosuwesabcusedcansssnanegee 16 Sample MOMVOGOSN SY Luer een 16 Effect of Gvapolallon suuasemaamuekefesbusejnteej es 17 Insufficient sample VOIUMG cecce cece cece tees ee ee eset ee eeeeeeeeeeed 17 7 TROUBLESHOOTING scsiceieneindicucceetaigoseetendiGtaweviiecaeeendneven 18 Q3000 driver installation CrrOr 0 ec ee ceeeeee eee ee ee ee ee esse renner nnnnnnne 18 USB connection GItOl sccccicei cscs ciddesiadsatcedietandevtew seaededesmeeceebandnewes 18 Sample accuracy and reproducibility ccceeeeeceeeeeeeeeeeeeeeeseeeeeeeeeeees 19 8 MAINTENANCE AND CALIBRATION rannnnnnnnvnnnnrnennnnennnnnnnnnnnnen 20 Maintenance uasmesmmnnetupuerenkmebgmnukutnd e iutjede 20 Check the calibration not available rrrnnnnnnrnvnnnnnnnnnnnnnnnnnnnnnnnnnnnn 20 T
10. orbance at 260 nm and 280 nm e mg ml Sample concentration in mg ml See Appendix C for more details on this calculation Measurement range BSA Concentration Reproducibility Absorbance accuracy 1mm 0 05 100 mg ml t 2 Abs 0 0 2 0 002abs Abs 0 2 1 0 02abs Sample Volume There is no specific requirement for the sample volume however for the best accuracy and reproducibility we recommend 1 5ul 15 Quawell Q3000 USER MANUAL 6 POTENTIAL SOURCES OF ERROR Sample overlap Sample residue on either measurement surface will affect the result We suggest that the user 1 Clean the two surfaces with dry soft lab paper after each sample measurement 2 Use 2 ul of de ionized water to clean the surfaces after measuring highly concentrated samples 3 Thoroughly clean the surfaces and surrounding area with de ionized water after completing all measurements 4 Before sample measurement blank the machine as described e Open the appropriate application module e Lift the upper arm and add 2 ul of blanking reagent to the lower surface e Lower the upper arm and click the Blank button e Lift the upper arm and clean the blanking reagent from both surfaces with de ionized water e Add 2 ul blanking reagent to the lower surface e Lower the upper arm and click the Measure button e lf the value is higher than 0 04 repeat steps b to f Sample homogeneity Non homogenous samples would cause s
11. rd Functions for Nucleic Acid and Protein A280 There are six function buttons for the applications of Nucleic Acid and Protein A280 f l Nucleic Acid Data Table Date 7 10 2011 No Sample ID ID SampleType A260 Sample ID My Sample gsUINA 1 Function button Exit exit the current application and back to the menu 2 Function button Quick Ref display the quick reference for sample measurement 10 Quawell Q3000 USER MANUAL Function button Blank measure and store the blank values for the sample to be measurement Function button Clear Data clear the data records on the Data Table Function button Export To Excel output the data on the Data Table to Excel spreadsheet Function button Measure measures and calculates the sample absorbance and concentration See Appendices B and C for details on the absorbance and concentration calculations Making measurement 1 Se N DD A Select the sample type and input the sample ID for the sample to be measured Open the upper arm add 1 5ul blank reagent on the lower surface and close the upper arm Click the button Blank Left the upper arm and clean the blank reagent from both upper and lower surfaces with the dry wrapper Add 1 5ul sample on the lower surface and close the upper arm Click the button Measure Clean the sample with dry wrap Export the data if you ne
12. sure button Quawell Q3000 USER MANUAL Patents and unique technologies e The liquid column technologies Patent No US 7 969 575 B2 Q3000 is built base on the following liquid column technologies Figure 1 Initially the sample is loaded and the upper arm is closed Figure 2 The upper arm is lifted by the actuator due to molecular effects the sample is broken into two separate parts one part is attached to lower surface and the other part is attached to upper surface Figure 3 After reaching its home position the upper arm is lowered A liquid column is formed when the upper arm is lowered to the position where the two droplets touch The measurement is then performed at this position without moving the upper arm upwardly again e CCE No Patent pending CCE No Calibration Coefficient is another unique technology used in Q3000 software With CCE No Q3000 has ability to 1 Automatically calibrate by running Diagnostic module option 2 Provide higher accuracy then the similar products in the market Quawell Q3000 USER MANUAL e The light system Patent pending The light system designed especially for Q3000 has many advantages 1 long life time 2 reproducibility 3 efficient Quawell Q3000 USER MANUAL 2 INSTALLATION Note Do not connect the instrument to the PC before software install Computer requirements e Microsoft Windows XP Vista or Windows7 e Microsoft Offic
13. ul 1 100 ng ul 2ul 2 gt 100 ng ul 2 Sample Volume There is no specific requirement for the sample volume however for the best accuracy and reproducibility we recommend 1 5ul 13 Quawell Q3000 USER MANUAL 5 PROTEIN A280 Protein A280 screen features Select Protein A280 from the Q3000 menu The module interface will appear on the screen r l Protein A280 tee Data Table Date 8 5 2011 No Sample ID ID Sample Type A260 A280 Sample ID My Sample SampleType 1Abs img ml v e Sample ID there are two windows that display the sample ID information The left window displays the sample ID input by the user The default is My Sample The right window displays the ID number for that sample ID The ID number begins at 1 and increases by 1 for each measurement Changing the Sample ID will automatically reset the ID number to 1 e Sample Type Chose the sample type for the sample to be measured There are 4 different sample types for protein measurement 1Abs 14 Quawell Q3000 USER MANUAL 1mg ml BSA IgG and Lysozyme The default is 1Abs 1mg ml The sample type is disabled after blanking The extinction coefficient of the sample selected will appear in the E Coe window e Ads260 Absorbance of the sample at 260 nm e Abs280 Absorbance of the sample at 280 nm e 260 280 Ratio of the abs
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