Data Acquisition Software User`s Manual Version 2.1
Contents
1. A Version 1 2 37 1 2014 NeuroWare User s Manual e Load Data into MatLAB Visit the NeuroExplorer website at http www neuroexplore r com code html to download the nex file reader writer function for MatLAB titled HowToReadAndWriteNexFilesInMatlab zip Copy the read NexFile functionin the archive to the fol der containing your recorded data In the MatLAB command window e xe cute readNexFi e SE CSC Ch nex where is the channel of your choi ce BALA PR Asi Fie Edt Debug Engel Dedi Wide Heb lid ASE AEE BY X Curt lA E Lie Sabati Ej eebe SN vg New Current eld OF x Work pece E a AHH 17 23 pue m ee Y HR LE BATLAE desktop keyboacd sbortcuts such en CtEl4E ere nr customisable Mind m ET In sddit nn man Feyocard skorrcurs Baye Changen for improves GoEsigrEEeg ET clei RR zl Schone the desktop cd aime _ Se istaniza Esthcard PAAR t s Er amp afarenzses From there vou CAP ARS rr3LOBRE previos arfmull ale IMI By fellowing Le FLERI cGublineS dm Hil Click bere if you de net want be set Chis message pala amt o iE gt gt pesdeaflie a Eer 3 adis Version 104 rummernrt 3k255 char Prag 30000 A Ebw gr il Lebar 1 6084T5 004 Suntvars Pet zelli SS P 3 E gt gt Command Hb y ADA TESEPIOI EMT png 2030 1147 J ena resrtTzl mar load Reccrded mat lomd recorded mar beer Be r ded mat Load peor mat load Be cccded2. Refer to the settings prouded under the Spike Display Settings tab to configure spike thresholdingand trigger conditions for each channel as the y are displa yed in the Main Window Use the Channel counter to the left of the Spike Display chart under the Main Window tab to cycle through your system s available channels Na viga te to the Spike Ch1 Ch16 Spike Ch17 Ch32 Spike Ch33 Ch48 and Spike Ch49 Ch64 tabs to simultaneously view those channels Main Window data streams This is a good way to quickl y determine whi ch channels still require trigger zoom filter adjustments Clicking the Clear Window button will dear the Spike Displa yand the corresponding Spike Ch tab chart of all detected spike waveforms This is for vewing purposes only and will not delete any recorded data Notch Filter Notch Filter can be applied to the raw data ofall channels from DAQ card It is recommended to review the Notch Filter Settings under the Filters tab before setting up spike triggering conditions and browsing spike and LFP wa veform NeuroWa re defaults the notch filtere nabled Yellow as shown below You can choose to disableitby dicking the Enable button NeuroWa re defaults the filtersettings to the ones shown below You can adjust the high low cutoff frequendes and orderany ti me Note You may adjust the cutoff frequency based on local power line frequency Version 1 2 15 1 2014 NeuroWare User s Manual Version 1 2 Filter Type
3. 1 2014 NeuroWare User s Manual H ex File Name Che File Name dec File Save Path CATBSlirecording file p J5 E Comment 256 Recording Data Selection 4 spike train vi spike waveform continuous data Continuous data select Raw Data e Start Stop Recording Itis recommended to review the Charted Channel File type Recording Data Selection and Record Duration before recording Record Duration is required to be a number with the unit Se cond which means you have to convert the recording time to time with the unit second Click Start Stop recording button tostart recording The recording process can be monitored either by Elapsed Time or by progress bar Whenever you want to stop recording before Record Duration run out you can dick Start Stop recordi ng e Howto Load Nex File The nex files generated by NeuroWare can be loaded directly into NeuroExplorer for analysis These binary files can also be loaded into Matlab please refer to the Appendix for details e Howto Load Text Data The txt file generated by NeuroWare is an ASCII file which can be loaded directlyinto MS Excel Version 1 2 30 1 2014 NeuroWare User s Manual Gi arcorded tet sr D n E F Vi Hi i K L M N i l sample rtie 30000 d 10 19 2010 17 27 amp cht ch 2 LGE ch A ens eh amp Ch d ch B chy eh 10 on 11 ch 12 ch 13 ch 14 ch 1 5 251801 2 5548 2521356 25267 252182 25204 531204 2 52504 2
4. 1 22135 251487 1 52636 1 53204 1 52747 2 531296 1 51914 1 53183 252453 2 53143 4 SL 255557 2 32208 2 53337 2 53174 255001 2 325379 252411 2 53784 2 52818 25304 252243 2 53087 2 53311 252561 ri Ax 2334 254823 15354 2352777 15244 152 506 232315 25418 25457 153007 1515031 15075 153571 15H43 1 al 2152716 152315 151815 253143 152472 1 54172 1 52055 2 351792 1 53159 2 53128 1 53104 153154 151196 2 51289 1 err 1 52716 2 515907 2 55877 2 53052 2 5264 2 535832 2 52182 2 52243 2 55052 1 52525 21 5322 23531206 2526735 251M9 2 mt 252655 2525 2153586 253036 252518 2 57747 251877 2 52909 25228 2 52426 2 5325 25023 153098 2 51556 L Events NeuroWare can detect the status change of up to 24 digital lines within the ADC DAQ board Digital level changes such as 010r 130 are thought of as an eventand can be displayed and recorded in NeuroWare The ADC cannot support simultaneous changes on different channels For this reason TBSI does not support the use of more than one Digital Data input at a time e Digital Lines Connection All 24 digital lines are accessible with a 68 pin SCSI connector A couple of digital lines are also accessible with BNC connector Connect the digital signals of interest to yoursystem via pins AO A7 BO B7 CO C7 shown below e Digital Events Di gital level changes of 24 digital lines are defined as different Event ID within NeuroWare see table and image below Any changes to the event variables A0
5. Rectangle D 2 Taps Taps 201 201 Version 1 2 17 1 2014 NeuroWare User s Manual e Spike Triggering Once you have finalized your Spike Filter Settings you can begin setting up the spike trigger conditions found within the Spike Display Settings tab Multi Channel Settings Recorded Channels Record Data Audio Output Threshold Levels C E n Ch amp TH1 uv TH2 uv Graph Settings Auto Trigger Settings Trigger TH1 ZEN Minimum Spike Width 2 B Pre Spike samples a E Threshold Settings Threshold Selection Thres hold and Threshold D Trigger Mode Tht First Iz Apply Current Threshald to All Channels e Audio Output Audio Output Ifanyspike is detected in the currentselected channel you will hear pu if Audio outputis enabled Use the oval button to enable disable audio output The audio corresponds to the channel currentl y displa yed in the Spike Display chart Drag the slidertoadjust the volume output Version 1 2 18 1 2014 NeuroWare User s Manual e Graph Settings Graph Settings DAQ Spike LFP Notch Filter Chart Display Reference Record Data Event System Gain Minimum Spike Window Display Threshold Level Spike Width E F E TURON j 3 THZ o j Min Spike Width Pre Spike Sam
6. User s Manual Start Data Acquisition After the configuration file has been loaded you can tell the program tostartacquiring data by clicking the Start DAQ button Starting the DAQdoes NOT initiate a recording session Stop H DAG 5 Neuro iz Ware Q own m Pireo ier PIO Sage e Tami Help EI Kess Le Leck lap Stop Mar Ana bike Chi Chia Ends MER Lio Oen ewer GA el DAQ ipe Zoom His ipa Display m y i Neuro Ware QQ quis regen 108 e 16 o Chere ati 1 Hdd Chisrsli Becgsg Chata fhe Deeply ete ELTE Etapa amp n TR E ul Audio Guiput ELUT AM m E D Icom Aj 1 Chiba Graph Letunga Acro Trigger being il Card 47 Eu Chert BI Wir SU Chant Zoom eier gre Date Celad kAtdke Becke Mire Width sd Timia el E ES Voie Warr rom D Enacpe Cha D Ak 81 ipp Luirg ri rylad ta A Chanas Chart Zoom Center Signal Data Select Display Mode Window Width ms i imiestamp 5 C ES Spike Waveform Scope Chart am 19 8333 Version 1 2 14 1 2014 NeuroWare User s Manual The raw data acquired by the DAQ will then be processed by NeuroWa re s digi tal spike and LFP filters if they are enabled under the Filters tab NeuroWa re s spike detecti on functions utilize the data generated by the designated spike filter settings Only one spike filtered channel can be displayed ata time within the Main Window tab
7. Bandpass Ei High Cutoff Freq fh roo sl Low Cutoff Freq fl 300 00 H Window wmm E Taps Filter Type Lowpass High Cutoff Freq fh 1 00 Low Cutoff Freq fl Filter Type Bandstop Ei High Cutoff Freq fh 55 00 Lei Low Cutoff Freq fl 45 00 adi s 1 2014 NeuroWare User s Manual Spike Detection and Display e Spike Filter Itis recommended to review the Spike Filter Settings underthe Filters tab before setting up spike triggering conditions NeuroWa re defaults the filtersettings to the ones shown below You can adjust the high and low cutoff frequencies any time before or during a recording A low cutoff frequency of more than 100Hzis recommended to remove the low frequency component signal Taps determine the order of the digital filter Selecting an odd number larger than 101 is recommended Multi Channel Settings Recorded Channels Record Data Spike Filter Settings LFP Filter Settings Notch Filter Settings Enable Enable Enable C B C E C E Filter Type Filter Type Filter Type Bandpass Lowpass D Bandstop d High Cutoff Freq fh High Cutoff Freq fh High Cutoff Freq fh 700000 5 1 00 55 00 Low Cutoff Freq fl Low Cutoff Freq fl Low Cutoff Freq fl 300 00 EI 300 00 EI 45 00 E Window Window Order Hanning
8. bara DAQ TESI P Munchen Chart Neuro Ware E cun Pegram Apis CAs pxas Sega Fit atente T Marianne Send Rage Cnannai Racer d DEA Chait Stings Version 1 2 25 1 2014 NeuroWare User s Manual Channel Reference Under the Reference tab click the oval Enable Referencing button to enable channel referendng These settings maybe adjusted during a recording if necessary and any changes will be reflected in real ti me When channel referencing is enabled the selected Reference Channel within the Reference Settings box will be applied to the channel currentl y being viewed in the Spike Display chart Click the Apply Current Reference to All Channels button to change every channel s reference channel or manually enter any channel numberinto the Channel Reference Chart to cha nge reference channel for indivi dual channel So you are able to select different channel as reference channel Note If Reference is enabled both Spike and LFP fil ter will function based on referenced data Multi Channel Settings Recorded Channels Record Data Spike Display Settings Filters Reference Channel Reference Chart Ch Reference Ch A Reference Settings Enable Reference Referencing Channel a gt a 1 5 3 4 5 Apply Current Reference 8 2 1 1 1 1 1 1 1 1 1 1 15 1 KH to All Channels Version 1 2 26 1 2014 NeuroWare User s Manual Record Data e Fil
9. devices e Run the newlyinstalled Instacal program Version 1 2 6 1 2014 NeuroWare User s Manual Q Set Program Access and Defaults Windows Catalog Windows Update Programs Accessories Microsoft Office Documents o LogMeIn Alim ISE Design Suite 11 fag Measurement Computing Search ei Mex Technologies Settings Instacal TracerDACQ E yt Ka Help and Support Run Log OFF Zeb Turn OFF Computer CG In e InInstacal you will see Board O USB 2533 serial under Universal Serial Bus Note Each DAQ boardis assigneda differentserial number but the boa rd numberin Insta cal should alwa ys be 0 InstaCal Fil Install Calibrate Test Help E If you can t see a device like this check the system s USB and power connections Version 1 2 7 1 2014 NeuroWare User s Manual e Right dick the DAQ board shown in the list then dick Configure Select 64 Single Ended in the field for No of Channels Click OK to accept these changes You may now dose the Instacal program window Board Configuration Cl en USB 2533 i InstaCal f OX Temperature Input Settings File S 359 Calibrate Test RR Serial E 345537 Add Board EZ 7 No of Channels EA Remove Board v al E EE T m iod Mo S Calibration Coefficients Factory Configure PCR Edge Rising Add Exp Board SAPCA Pin Direction Input Add Mem
10. mst lone Be zrf0e nt D mae spencer Li d Sen 811 20 re E terohg rrneHrxF li rede m renber E ntrkchse r ai nes fie and GA Tree Sr l f ratum His des eu pEEkGD rEcadHeEF Eli In the MatLAB workspa ce double dick the variable labeled ans The Variable Editor window will appear Version 1 2 38 1 2014 NeuroWare User s Manual In the Variable Editor double dick contvars MATLAB 7 97 0 PT CTE his Alju ani UAT Ch 040 OTe 17 25 30 BE 260 Q ing A e ome ll litt In wu mm e BIDBESJD x M T MN pre m SEE EE Hana e Lee det g r EE E D T PE EC e i Mi re la Bac ze pm m LA umm MI e NH a nex Dap Aa cho run Sp N ASIZOID 11147 A Lec E HA dond teen Tal mat PE arch ne In WSO 10 03 e OCA mc Jeng Decorded mat PM 3i T5 Chus wen ined recorded ar CR OEA Load Peccrded mec n SSC nn toed Reccrded met P IE Ipheslh re Led hwruiiid eat edere tru iont Rccarded mat epa D nexcbjereadiexFils Cel te m MATLAB Taube gt mites 3 19 E010 2 00 F nerdbi readHewFils table e racc cd im le en us Pla nd T A T rT eme pu m 3 12 2010 2 41 F AR E H ve EX Version 1 2 39 1 2014 E j Select data to plot fi i Ji E r Stack Base E EET LU E e Bo SOS GO ROS ESSE EIS EISIESISISS SISSE ga Variab
11. of all channels from DAQ card The default cutoff frequencies are setaround 60Hz rejection It is recommended to reviewthe Notch Filter Settings under the Filters tab before settingup spike triggering conditions and browsing spike and LFP wa vefom Filter Selection The default filter is Butte rworth filter e Chart Display Tab Display Mode Strip Display Mode Strip Chart displays the selected channel s data as a constantly scrolling stream Scope Chart refreshes entire window with new data after chartis filled Sweep Chart same as scope chart but continues to display previous data until itis overwritten by newer da ta Multiple chs Window Config Window Width ms adjusts the maximum amount of x da ta displayed ata time Display Zoom increases or decreases the zoom of the Y axis Strip Window config Data Select Raw Data displays the incoming raw data unfilte red Spike Waveform displays the selected channel with spike filter setti ngs applied LFP Waveform displays the selected channel with LFP filtersettings applied e Reference Tab Neuroware is set default to reference from the headsta ge AGND which should be connected to an animal ground implant If you wish toinstead use differential referencing from a sped fic recorded channel dick the oval Enable Referencing button When enabled the green lighton the oval button will be lit Reference Channel Setting This function isalso available through the Reference Tab in Neuroware For eac
12. 3 22 57 Documents default For Meuroware 2 0 Single T1 c 9 15 yp 3 default For Meuroware 2 0 Single T3 rm default For Neuroware 2 0 Single W5 ini default For Meuroware 42 0 Single W16 ini A default For Meuroware 42 0 Single 432 ini A default For Meuroware 2 0 Single W64 1 ini Desktop Neuroware Configuration ini My Documents Mu Computer File name default for Weuroware V2 0 Single M 32 m bul Im hu Network Files of type Custom Pattern ini e e Configure NeuroWare Adjusting Settings in a Configuration File To change and save selected DAQ settings to be loaded in future instances of NeuroWa re navigate to the Tools menu at the top left of the program then select Configuration from the drop down menu to open the Configuration Dialog Many settings in the Configuration Dialog are unique to that menu and can t be accessible in NeuroWare Itis recommended to reviewall settings in the Configuration Dialog to ensure that each parameteris appropriatel y defined Refer to other sections for how to set up those settings thatare only accessible in Configuration Be sure tostop DAQ before opening the configuration dialog e Load New Config Use this button when you want to load a new configuration file Version 1 2 9 1 2014 NeuroWare User s Manual e Save Use this button to overwrite the default configuration file provided by TBSI or Save As to open a prompt forsaving a ne
13. 352716 25151 251617 2 52MM 2 5312 2 52396 Ai 6 151785 25075 251564 251457 1 52304 2 51594 1 53418 2 52518 1 52818 1 52208 1 52457 251319 25383 251507 1 d dAn 2 52991 2 52355 2 531036 2 52509 2 52914 253662 2 52585 252197 2 52243 2 5293 252335 2 53876 2 5264 a 5 15336 15134 1253231 25331 2524187 2 5214 253972 2573701 152594 25211 252534 2525800 21533355 25367 Li 2 2 52853 2531326 252716 2 53157 1 52823 252116 2 93281 1 52538 2 532155 25154 1 52518 53052 2152143 15235 L 10 1 52808 2 53799 2 504537 2 53403 1 528203 2 52747 2 52885 1 5254 2155022 2 52423 253472 2 52475 12 5294 2 52414 2 11 232853 253833 25249 251026 524472 253372 SSM 2520665 1523335 252625 5521 152704 12153983 3215332 d 1j 1 53196 2 541869 2 5115 1 53187 2513 2 53418 2 353067 2 52441 1 526025 2 513914 25156 252731 1 53967 2 52525 1 13 23174 252991 452147 2 33388 2 31580 2 53113 2 53098 25354 233129 2 52747 2151093 352014 25252735 231952 d 34 153555 1253525 253337 1 53403 2531153 2 53 25 975 2518 252890 21529495 252701 2 53000 153326 2 525055 Li EE l53113 2 533172 1 52975 2 51586 1 52487 2 53006 252808 253632 2 52258 253006 252 47 1 530360 1 53403 2 53494 21 185 253453 2 53174 2 52833 2 543952 2 52777 2 52809 253808 2 524414 257122 253067 2 52747 25267 2 53818 2 52833 Z 17 1531539 253128 533106 153279 252335 252625 1 52869 25375 251548 251555 2 5325 254072 15551 1 5285 l ij 253418 253011 2539265 21 5314 3
14. A7 BOB7 COS C7 will be shown in the Event Table Forexample if the digital level of the AOline changes you will see an eventin the Event Table as shown below Version 1 2 31 1 2014 NeuroWare User s Manual Event Definition Table Event table Timestamp Port status Event ID Event String EX 138030 00000000000000000000 10 AD low 12 8030 A0 high 11 8030 00000000000000000000 10 A0 low 10 8020 10000000000000000000 11 A0 high 10 3020 00000000000000000000 10 A0 low 8 3020 A0_high The Event String can be changed to better describe the details of the event You can define your own Event Definition Table by the page of Event of Configuration Dialog as shown below Event Definition Table the default string is named as PortNum_High PortNum_Low Max Event Num the maxnumber of events that s displayedin Event Table It s for display purpose only Max Event Interval ms the min width of pulse that can be detected as digital event is decided by this number You are able to change it down to 1ms while the default is 100ms but the measure ment difference of tens of ms ma y still be seen depending on your computer performance Note Event Definition Table can t be edited in NeuroWare Version 1 2 32 1 2014 NeuroWare User s Manual e Event File Digital event i
15. Ch 1 64 windows of all detected spike waveforms This will not delete any recorded data Channel will select which channel is shown Spike Display and Strip Chart Spike Channels Tabs Each of the Spike ChX ChX panels containup to 16 individual spike channel windows Version 1 2 21 1 2014 NeuroWare User s Manual Error Log Optillap Man Window Spike Ch1 Ch15 Spike Ch17 Ch32 Mulli Channel Viewer e Clear All Chamel Windows i Charnel 1 Channel 2 Charnel 3 Channel 4 The same spikes detected by the thresholds from the Main Window are shown in these small charts dlicking the Clear All Channel Windows button will dear the Spike Display and the Spike Ch 1 64 tabs of all detected spike waveforms This is for viewing purposes only and will not delete any recorded data Strip Chart The Strip Chart is located at the bottom of the Main Window tab and displays the real time waveform of the channel selected for display within the Spike Display window Version 1 2 22 1 2014 NeuroWare User s Manual Chart Zoom Center Signal Data Select Display Mode Window Width ms Timestamp s 10 C m Spike Waveform Scope Chart D 400 17 100 00 e 50 00 0 00 01 50 00 0 01 100 00 Center Signal Click the oval Center Signal button to automatically center the incoming signal to OV Data Select Raw Data displays the incoming raw data unfilte
16. E r Ls se P N e e l pa F e 3 NN E A pl JE Data Acquisition Software User s Manual Version 2 1 Triangle BioSystems International 2224 Page Rd Suite 108 Durham NC 27703 Phone 919 361 2663 e Fax 919 544 3061 www trianglebiosystems com NeuroWare User s Manual Contents Blerstigo iquES leuis ias 3 SVS HO ONEVIEW MNT Tm 3 Standard Shipped items Iert 4 System Ten EC 4 SAE O ER uc 4 A O02 a2 RA RE 5 e Ee Te t nr ee nn ane eee eras 5 A as E oo PEO eee 5 Hoo 5 e ET wae es ce ree emanate eins semen one seen aaeeeeeaes ccs 5 s NSB Bic m 5 e OND APP E O O en ere ee eer ere ert eer ee er ee re 5 Jo o eee nent ect ee mere ene oo A 5 2088 55 5 50 0 IC ao A IP E Eo EE 5 NAAA 6 EO ACIS AiG COAH SU e RR 8 e lud UTI Umm 8 e Loada Configuration bie 8 e Configure Neurouwaref antha anta arina n Ennan rnan ENEA Da EEEn PEE Enn nananana annann a 9 e C c Eee mm 9 ZEE E 10 green 10 A M D I ee 10 AP emet dU aM E 10 EE ui cr 11 MEM cio a o II nectnnetnedeosacneradacaesuaasenseceecct 12 Chart Display Ta m 12 EE ECK oie 12 e RECCO Data grow ee 12 R cord Data Ta c 13 Version 1 2 1 2014 NeuroWare User s Manual Ee E EC a e s 13 stare D
17. Spike Ch nex spike wave form detected by NeuroWa re threshold on channel SE Spike TS Ch nex Timestamp train of detected spikes on channel Analog Input Ch nex Analog signal from BNC connector Version 1 2 27 1 2014 NeuroWare User s Manual 2011 03 13_13 37 22 Pe Et Ven Favorkes Took Hep Gre QF Lowe rm CS Sinz Wike CO CATISTgecording Flelz01 LL 13 37 22 FU a ee PNIFGDSDAIN rer side EX d aD ng PASE o AA res ce A nie PASE pe rex cl SE SoS ee vi A nec PESE Spiks h5 teen PASE Spdos Cie nex ce A A cl re al Zr e Chines he Z h r PASE Sal lanx PASE Gre hi nex he Spe nex PMSE Spke Ch140 a a a rex PASE Spee a 0h17 ee a a a rex lo EA a A ne RGL Geet re PASE pe Chad rer FECE ZI anh iux Deg CSC ach p nex de A rs e E 3 nex de a de NN PU A DE CSC ChlD res Dsg Ca OS fi a Uu In mes PN SEC Chur pex ae A S a ho ms PM SEC Cheb rex e File Save Path NeuroWa re defaults the file path for recorded file to CXTBSI Me cording file Change the file path before start recording if needed e Recording Data Selection If NexFile is selected Spi ke Train is selected by default Spike Waveform Continuous Dataand ME sols T5 chUD nes P3 Se eEg T5 Cho ax FW SE Sob TS fex Me code TECH ms A Se eg T5 ChL 8 nx SE pde T5 cha nex E FE See TALA pes A A ee T pui FW SE See TEC EB ex Analog Input Channel are ready for being selected If C
18. a System Overview Receiver AD Q za E Neuro iz Ware Data Certified Headstage Transmitter NeuroWare is designed to interface seamlessly with all TBSI tethered and wireless shown above neural recording systems Version 1 2 3 1 2014 NeuroWare User s Manual Standard Shipped items list 1 Wireless headsta ge 1 Magnet on off wand 1 Wireless receiver demux base station 2 receiverantenna 2 receiver antenna extension cables 2 antenna clamps 1 USB A B cable 1 6v power supply 1 heads tage charger 1 heads tage test cable 1 heads tage shorting plug System Requirements The recommended minimum PC requirements are as follows Atleasti7 Quad Core Processor 8 GB RAM DDR1333 or higher 512MB of video memory C drive OS SATA 2 3GB S or faster 250GB or greater D drive storage SATA 3 6GB S SSD 500GB or greater RECOMMENDED HARDWARE IS A SATA 3 6GB S SAMSUNG EVO SSD 500GB OR LARGER USB 2 0 port Wi ndows 7 64 bit OS System I O Version 1 2 4 1 2014 NeuroWare User s Manual e POWER INPUT This is to be connected to the 6V DC powersupply provided by TBSI onl y e Analog Input TTLinput 5V 30kS s DATA A Digital Events input DATA B Digital Events input USB A Primary ADCI O connection USB B Secondary ADCI O connection GND Earth ground connection lug DATA I O Not supported e Headstage input pinout refer to appropriate chan
19. ata A SO Mmm EE 14 Vier dla EE o 15 Spike Detection and DISDIAY siria aces 17 Spike EE 17 EE EISEN 18 s Aao Pigeon 18 B ic cia sud NUT 19 o AUTO VEER 19 A nn PI I 20 Spike Channels E a nas 21 St Gg EE 22 Ree ed aaa eaito 23 Multi Channel Viewer 24 Channel eflet eli EE 26 issu 27 e FETIP ien nemus On A M E 27 e o e paper 28 o Recorame BEE ra E 28 E e eu cem E OA A II 28 e Recorded Channels escitas tratada 28 e Recorded PilehName A 29 JS inser e Te 9 Tm 30 SEN e ee Be DR CI T 30 gt Howto Load Text Dota scudo 30 SE M 31 EENEG 31 AER dc AU EE 31 e EVENTO poo 33 Ana loc ipe UIE B aT IRE C RTT TET T TORTE 34 o LOS WIN Westin et 35 Version 1 2 2 1 2014 NeuroWare User s Manual CUTE NOLON tesoro E N E OS E N P 35 as a Eu A A 36 e Load Data into Ne roExpl oret scene 36 e Load Data LEE E 38 ape SUDON GE 41 Contact Informati ON RE 41 Version history for this manud ENT TOT TT 41 Document Overview This document is intended to educate users about the full functionality of TBSI s online data acquisition software NeuroWare has the capability to graphically display and store up to 32 simultaneous channels of neural dat
20. e Types IT IS IMPORTANT THAT CUSTOMERS RECORDING MULTIPLE SIMULTANIOUS CONTINUOUS DATA TYPES CHANGE THE RECORD FILE LOCATION TO A D DRIVE RECOMMENDED HARDWARE IS A SATA 3 6GB S SAMSUNG EVO SSD 500GB OR LARGER Nex File a type of binary file with predefined file header and data body Multi Channel Settings Recorded Channels Record Data File Type Recording Data Selection Mex File File Save Path Spike Train CATBShrecording file Spike Waveform Record Duration s UD 5 Continuous Data Type Comment Raw Data Analog Input Channel Spike Display Settings Filters Multi Channel Settings Recorded Channels Reference Record Data File Type Recording Data Selection Tat File Ei File Save Path a p ike Train CATBSIrecording file i Spike Waveform HOME AES Continuous Data 5 Continuous Data Type Comment Raw Data Analog Input Channel Start time Start time End time End time Elapsed Time s Elapsed Time s 0 D Po Po If Nex File is selected Seven types of data maybe generated by NeuroWare aftera recordingis complete upon the selected options in Recording Data Selection Event nex Digital event log ex lever press or audio tone SE CSC LFP Ch nex Continuous LFP filtered and referenced neural signal from channel SE CSC RAW Ch nex Continuous neural signal referenced from AGND channel SE CSC SPIKE Ch nex Continuous SPIKE filtered and referenced neural signal from channel SE
21. h channel you are able to set up separate reference channels for Spike data and LFP data recordings e Record Data Tab File save path The default file path is CXTBSINrecording file However itis highly recommended that you change this path to an appropriately sized SATA 3 Solid state storage drive orhigh speed RAID arra y Recording Data Selection You can select to record simultaneous spike wa veform LFP and continuous raw data by checking associated boxes Spike chain is checked by default Comments You can add additional information to recording files before starting record Recording time Use this box toset the duration in seconds of your recordings Recorded channel setting Setup if the channel is recorded and select what type of continuous data are selected Version 1 2 12 1 2014 NeuroWare User s Manual e Record Data Tab Max Event num Thisis the max number of events showed in Event Table For example ifwe setit up to 50 the 51 eventwill be displayed on the firstline of Event Table Min Event Interval This boxsets the minimum time expected for an event to take place Default this is set to 100mS If more than one eventinputis to be recorded this needs to be set to 1 Event Definition Table Use this table to change the definition of the events recorded e System Gain Tab System Gain This displays the gain applied to the signal acquired by the DAQ This cannotbe changed Version 1 2 13 1 2014 NeuroWare
22. here are two threshold are available tosetup the threshold levels Threshold1 Only Threshold1 is enabled Threshold2 Only Threshold2 is enabled Threshold1 and Threshold2 Both Threshold 1 and Threshold2 are enabled Only Spikes crossing Threshold1and Threshold2 will be detected Threshold1 or Threshold2 Both Threshold1 and Threshold2 are enabled Spike crossing either Threshold1 or Threshold2 will be detected Trigger Oder only available when Threshold1 and Threshold2is selected in Threshold Selection Threshold Level This displays the amplitude in uV Thateach channels Threshold is set at e LFP Tab LFP sample rate Thisis the sample rate at which LFPsignal is acquired and displayed Since LFPs do not require as high a sample rate as Spike recordings this tab gives you the option to sample LFPs ata much lower sample rate LFP filter Alowpass filter is used to remove unwanted high frequencynoise and signals Adjust filte rbandwidths by changing low cutfrequency The high cut frequency will be ignored when you use the lowpass filter The taps is the orderof digital filter An odd number more than 101 is recommended The window option affects the digital filte r pe formance If the dick to enable button is on the current filteris used If the Click to enable button is of the current filter is off Version 1 2 11 1 2014 NeuroWare User s Manual e Notch Filter Tab A Notch Filter can be applied to the raw data
23. ignored but you can change the low cutoff frequencyatany time even during an experiment LFP signals are of a much lower frequency than spikes so three lower sampling rates 2K s 1K s and 7 5K s are available But the sample rate for LFP can only be accessible in Configuration Dialog and you can t change them when DAQis going DAQ Spike UFP Motch Pe Chart Display Reference Record Data Event Multi Channel Viewer Every channel s real time wa veform can be viewed within the Multi Channel Viewer page Channels ma y be added or removed from the Charted Chart in the Multi Channel Settings tab A channel legend can be found to the left of the M C chart Version 1 2 24 1 2014 NeuroWare User s Manual Charted Channels Selectthe channels that are displa yed in Multi Channel by Add or Remove buttons Center Signal Click the oval Center Signal button to automatically center the incoming signal to OV Continuous Data Type Raw Data displays the incoming raw data unfiltered Spike Waveform displays the selected channel with spike filter setti ngs applied LFP Waveform displays the selected channel with LFP filtersettings applied Window Width ms adjusts the maximum amount of x da ta displayed ata time Zoom increases or decreases the zoom of the Y axis Interval adjust the interval among the channels of Y axis Ema Log EHM m ken kd anri fecht Sp Chieh Bp L n nas Mui Canna gege
24. le Editor ans contvars Double dick the 1x1 struct cell to extract the data content n Le gt uo pe e N 9 be o i gt U Z 1 2014 40 Version 1 2 NeuroWare User s Manual BEE yax ga Variable Editor ans contvars 1 1 File Edit View Graphics Debug Desktop Window Help BI A Es Fel a te tg Stack Base KO mo valid plots For ans contva e HH LI EJ Gi al E ans contyars 1 1 1x1 struck Field value name Con Chi 0 Varversion 101 101 101 ADE o 1 1 1 MyOF set 6 0135e 154 56 013 5 013 ADFrequency 30000 30000 30000 timestamps 3 265383 3 265 23 255 fragme lt 10000x1 double gt 56 013 2553 2 ans x ans conbvars x ans contvars 1 1 x Technical Support All technical support and application questions should be directed to John Mcintyre at Triangle BioSystems Contact Information Phone 919 361 2663 Fax 919 544 3061 http trianglebiosystems com contact us html Version history for this manual 1 0 Initial release with Specifications 11 2013 1 1 Removed functionality table add ed I O 1 2014 1 2 Added info for config dialog box 1 2014 Version 1 2 41 1 2014
25. nel ha rd ware manual Version 1 2 5 1 2014 NeuroWare User s Manual NeuroWare Installation Install the driver for the USB 2533 DAQ card e Navigate to CD drive NeuroWare DAQ Drivers and run mccdaq exe e Follow the prompts to install the DAQ drivers and the Insta cal and Tra cerDAQ softwa re tools e You may be required to restart your computer once installation is complete Install NeuroWare e Navigate to CD drive NeuroWare Installer Volume and run the file labeled setup exe e Follow the prompts to install the NeuroWa re software Copy files from Installation CD to PC e Copy the TBSI folder on the installation CD to the C drive on your PC C XTBSI File Edit View Favorites Tools Help C9 pack T a yo Search Folders EBk amp a Folder Sync TAC II gt File and Folder Tasks e Make anew Folder backup configure File recording file Error Log txt ed Publish this Folder Eo the Web KI Share this folder Other Places ya Local Disk C E My Documents CO Shared Documents d My Computer a My Network Places Configure the DAQ card s channel number in Instacal e Connect the receiver to your computer with the USB cable e Your computer will detect this as a new device and install additional drivers automatically e Once the drivers are installed you ma y navigate to the Device Manageron your computerand check to see if DAS COMPONENT USB 2533 is include d the list of available
26. nformation stored within the event nex file shown below can be loaded into NeuroExplorer Version 1 2 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 3 2012 12 29 PM 5 2 2012 12 20 DAA 33 NEA File MEX File MEX File MEX File NEX File NEX File NEX File MEX File NEX File MEN File 2 032 KB 864 KB 864 KE 864 KB 864 KB 864 KB 864 KB 864 KB 864 KB 1 2014 NeuroWare User s Manual Dur aen 0 00 01 File Suse il 2 081 Frigia 20000 heures E nz l Pon V cgergr Bhar lure i fi Wurst r i ee Cont gg D bere Variis hee beson bere Mocho ss Poste Variable T stampz Count Hean Freg Hibe w l Char Pe a 2 000 A niri lad 1 000 o i AVariablez Tineztamps A Intervals Vaveforaz Pop Y Analog Input Channel An analog input channel separated from the other neural channels is provided for custom purpose The TTL waveform up to 5V can be acquired by DAQ directlyatup to 30kS s Also no filter will be applied to this channel Analog Input V AC Couple Window Width ms Y Zoom C E 10 10 E a P fd a NN LI L4 LJ 0 1 L a LI LS LI LJ E 0 01 Version 1 2 34 1 2014 NeuroWare User s Manual Error Log Window The Error Log window helps trace problems that have occurred within NeuroWare Please consult this page if yo
27. ontinuous Data is checked you geta chance to select what kind of data is recorded Raw Data the incoming raw data unfiltered Spike Waveform data with spike filter settings applied LFP Waveform data with LFP filter settings applied Analog Input Channel is particularly for BNCa nalog input e Comments Any information less than 256 char typed in Comment will be not onl y sa ved in Nex file but Eu SE age Ba Chig rer SE Spike a SE Sole TS bl rex Spee SE Spot T5 Chz3 rex GE Ze Te hes npe SE Spike T3 Oha S rer SF Spot T5 Chz E rex GE hdd 15027 nex SE Spike T3478 rez SE Ze SZ Th mex GE pd Te Ch OL inc SE Spb TSG nw Ed Temp Filet For Con TS kmp El Teo fie for Con Num Pre alsoin Note txt file which is in the same folder as Nexor Txt file e Recorded Channel All channels will be recorded by default You canadd or remove the channel by dicking Remove or Add button in Recorded Channels tab Version 1 2 NeuroWare User s Manual Multi Channel Settings Recorded Channels Record Data Available Channels Recorded Channels Description A Description e Recorded File Name By de fault the Nex files are na med as SE CSC Ch _ nex SE Spike Ch _ nex SE Spike TS Ch _ If you want to change them you can go to Configuration Dialog to add suffi x to ea ch channel For example if you type in Neuron1 in Ch1 and Neuron2 in Ch2 the recorded file na me will be SE CSC Ch1 Neuron1 nex Version 1 2 29
28. ory Board 533 serial 345537 XDPCR Edge Rising Add MetraBus Board AA XDPCR Pin Direction Input y Remove All Instatal AutoRun ADC Settling Time 1u ADC Maximum Hate 1 MHz Performance T est Adc Fito Input Speed Digital 1 0 Input Speed Cancel Configure board settings E E E A screen resolution of atleast 1280x1024 is recommended to run NeuroWa re Loading and Configuration e Start NeuroWare You can run NeuroWare via the Windows Start Menu by navigating to Start All Programs NeuroWare If there is no right license file named as Ne uroWa re li cence dat in C ATBSIA configure file NeuroWa re may run in demo mode In demo mode Recording is disabled e Load a Configuration File Upon loading Neuro Ware for the first ti me you will be asked to select a configuration file Each configuration file contains progra m functionality settings fora spedfic TBSI recording device Version 1 2 8 1 2014 NeuroWare User s Manual Choose the configuration file which matches your data acquisition hardware model ex a file ending in M32 should be used with a 32 channel M Series recordingsystem Enter Configuration Filename ini Look in 3 configure file v O eg 4 default Far Meuroware 42 0 Single M3z ini default For Meuroware 42 0 Single T5 ini Mu Recent default For Neuroware V2 0 Single Ta TYPE Configuration Settings l Date Modified 2013 4
29. ples Channel Selection Che TH1 uv TH2 uv A St J2 S 20 1 1 Pre Spike E k 2 Samples indows Samples Spike Repeat 200 3 2 PR z 100 E D p 5 Note Windows Samples and Spike Repeat lt 200 are onlyaccessible in Configuration Dialog and you can t change them when DAQ is going Minimum Spike Width the minimum number of DAQ samples required to cross the threshold s to allow that portion of data to be registered as a spike Pre Spike Samples the number of samples the spike chart displa ys to the left of the triggered data Windows Samples the number of samples the Spike Display Window Spike Repeat lt 200 the number of detected spike wa ve form ove rapped in the Spike Display Window e Auto Trigger Settings Trigger Settings are located in Spike Display Settings tab Auto Trigger Settings Trigger e RMS RMS Auto triggering applies toall channels and will take a few seconds to complete asit processes samples from the current channel s data Gicking the Auto trigger button will automati callyset up triggerthreshold s for every available channel which can be useful for qui ckl y identify ng Version 1 2 19 1 2014 NeuroWare User s Manual possible spikes onany given channel This functionsets the spike threshold s by calculating each channel s root mean square RMS voltage and multiplying it by a user defined rational number If you wish to use auto triggering wi
30. red Spike Waveform displays the selected channel with spike filter setti ngs applied LFP Waveform displays the selected channel with LFP filtersettings applied Display Mode Strip Chart displays the selected channel s data as a constantly scrolling stream Scope Chart refreshes entire window with new data after chartis filled Sweep Chart same as scope chart but continues to display previous data until itis overwritten by newer da ta Window Width ms adjusts the maximum amount of x da ta displayed ata time Chart zoom increases or decreases the zoom of the Y axis LFP Waveform LFP waveform can be viewed byStrip Chart or Multi Channels Viewer when selecting the right data type Itis recommended to review the Filter tab before viewing LFP NeuroWare defaults the filter settings to the ones shown as follows Version 1 2 23 1 2014 NeuroWare User s Manual Multi Channel Settings Recorded Channels Record Data Spike Filter Settings LFP Filter Settings Notch Filter Settings Enable Enable Enable C B C B C B Filter Type Filter Type 7 Filter Type Bandpass Lowpass DN Bandstop High Cutoff Freq fh High Cutoff Freq fh High Cutoff Freq fh 700000 j 1 00 55 00 Bl Low Cutoff Freq fl Low Cutoff Freq fl Low Cutoff Freq fl 30000 E 200 E 45 00 El Window Window Order Hanning ell Rectangle 2 Taps Taps The high cutoff frequency will be
31. shald1 Tiinaer Mode IRE Threshold2 e Lilli Threshold and Threshold2 m _ Threshold or Threshold2 dicio Apply Current Threshold Apply Current Threshold The First Both Th1 and Th2 to All Channels to All Channels Version 1 2 20 1 2014 NeuroWare User s Manual The manually adjustable spike trigger thresholds apply only to the channel currently displayed in the Spike Display chart These two threshold sliders green and yellow found on either side of the chart update the current channel s trigger settings in real time Simply drag the sliders up and down orentera new value into the Threshold Levels table found in the Spike Display Settings tab to control the individual positions of the thresholds Eno L pd a goung 5 Siap han iere apks Chi dcnid Bri Catches Mi Cara Va EL Neuro Ware L Get program my Mum cnan Seer Recorded Cres Recora Data Seite Dupuy Sings E r Feiert heard Serra Tea Dieron The marecd amd ertha m Cha fom CN Signa Dia ieee Lavpiar Lode Pange Ver mua Tare size ai Pri res Soph Visit iris CE Ar y 117 5 TOO ERO Tren oda i n1 Firil BI DD i 007 Ape Carim rei tz ab Chanel HM 501 120 00 Spike Zoom slider will increase or decrease the vertical zoom within the Spike Display window If Zoom All Channels is checked Spike Zoom will be applied to all Spike windows Clicking the Clear Window button will dear the Spike Displayand the Spike
32. th both available thresholds TH1 and TH2 you must enable them byselecting Threshold1 and Thres hol d2 from the dropdown listin the Threshold Selection of Threshold Settings section found in the same tab e Threshold Settings Two threshold sliders green and yellow are available toset up the threshold levels Si mpl y drag the sliders up and down to control the thresholds Threshold1 green cursor is used to detect positive spikes Threshold2 yellow cursor is used to detect negative spikes Threshold Selection Threshold1 Only Threshold1 is enabled Threshold2 Only Threshold2 is enabled Threshold1 and Threshold2 Both Threshold 1 and Threshold2 are enabled Spike crossing Threshold1and Threshold2 will be detected Threshold1 or Threshold2 Both Threshold 1 and Threshold2 are enabled Spike crossing either Threshold1 or Threshold2 will be detected Trigger Mode onl yavailable when Threshold1 and Threshold2 is selecte din Threshold Selection Th1 First Spike crossing Threshold1 first and then Threshold2 is detected and displayed Th2 First Spike crossing Threshold2 first and then Threshold1 is detected and displayed Both Th1 and Th2 Anyspike crossing both Threshold1 and Threshold2 is detected and displayed Threshold Settings Threshold Settings Threshold Settings Threshold Selection Threshold Selection Threshold Selection Threshold1 and Threshold D Threshold1 and Threshold2 D Threshold1 and Threshold2 Thre
33. u experience problems during recording All errors are logged into a txt file located in the directory spedfied within the Error Log File Path field Mir Var o Spike Ch1 Ch16 Speke Cn17 4ch32 Mufs C hannel Wiewer kvants Ener Log OpliMap Errar Leg Quit Neuro Ware To quit the program dick the Quit Program button which is located beneath the Start DAQ button or dose panel directly It s recommended to check if you like to save the current setting to the current config file orsave as a new config file by Configuration Dialog Tools Con figuration Neuro Ware Stop BR o Version 1 2 35 1 2014 NeuroWare User s Manual Click OK in the next dialog box to confirm that you want to quit iem Are you sure you wantto quit Reading files e Load Data into NeuroExplorer Load the NeuroExplorer program navigate to File Open and open SE CSC Ch nex where is the channel of your choice d Hpuzetspkrer IS LSC Chi rea lada A j oo ie PEDE Ga 18 a E D ERT e e um db WU Le ki pu A kt DE TA as Eh HB A P i ia pe KE Zei WI E b g e K EN 2 4 Version 1 2 36 1 2014 NeuroWare User s Manual a amp Heureioplarer 8 Spike Chi mez Dala ad A A md uni A E o Sange L 3 ee Steed Window Hein V curiales j i AAA E B HE EE EH
34. w configuration file e Returnto NeuroWare Use this button when you have completed and sa vedall changes to your configuration file e Daqtab Under the DAQ tab of the Configuration Dialog you will see a mapping of the physical channels this cannot be edited and the Sample Rate drop down box The default sample rate is 30k cycles second which is the ideal sample rate forspike recordings This can belowered significantly if only LFP data will be recorded FS Configuration Dialog V1 8 vi DAQ Spike LFP Notch Filter Char Display Reference Record Data Event System Gain Fissi Y ro j Ware eg kb with sangle DACH e is 15 0005amples5 when 30 000 is selected e Spike Tab Minimum Spike Width Sets the mini mum number of DAQsamples required to cross the threshold s to allow that portion of data to be registered as a spike Pre Spike Samples the number of samples the spike chart displa ys to the left of the triggered data Windows Samples This is the number of samples shown in the Spike Display Window Spike Repeat 200 the number of detected spike waveform ove rapped in the Spike Display Version 1 2 10 1 2014 NeuroWare User s Manual Window Spike Filter A low cutoff frequency of more than 100Hzis recommended to remove the low frequency component signal Taps determine the order of the digital filter Selecting an odd number larger than 101 is recommended Threshold Selection T
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