User Manual
Contents
1. The diopter adjustment has not been performed Perform the diopter adjustment p 9 After changing the objective the specimen goes out of focus The diopter adjustment has not been performed Perform the diopter adjustment p 9 The images viewed through the binocular eyepieces do not merge into one The interpupillary adjustment has not been performed Perform the interpupillary adjustment p 9 The diopter adjustment has not been performed Perform the diopter adjustment p 9 Eyes get tired during observations The interpupillary adjustment has not been performed Perform the interpupillary adjustment p 9 The diopter adjustment has not been performed Perform the diopter adjustment on the right and left eyepieces p 9 The brightness is insufficient Adjust the environmental light The coarse focus knob and the coarse stage height adjustment knob are too tight The torque adjustment knob is tightened extremely Loosen the knob properly p 12 The specimen goes out of focus over time The zoom body or the stage descends with its own weight A torque adjustment knob is loosened Tighten the coarse torque adjustment knobs to increase the torque so that the zoom body and the stage do not descending with their own weight p 12 23 Care and Maintenance Cleaning the Lenses Keep the lens from dust particles or fingerprints2. To perform the differential interference contrast DIC microscopy remove the cover of the slot and attach a slider suitable to the microscopy method and the illumination method The slider must be selected depending on the illumination method the episcopic illumination or the diascopic illumination To perform the DIC microscopy an illuminator for the episcopic illumination or the diascopic illumination is required For the procedure of the DIC microscopy see the instruction manual for the AZ ICI Coaxial Episcopic Illuminator or the instruction manual for the AZ STD Diascopic Stand and the AZ STGD DIA Stage Attaching the DIC prism slider For the episcopic illumination T Attach the AZ PH EPI DIC Prism Holder to the slot on the mu AZ100 and insert a DIC prism slider for the episcopic illumination into the slot on the prism holder Select a DIC prism a slider suitable to the objective in use Refer to the following C table j For the diascopic illumination E Two DIC prisms are required The first prism is inserted to the E position above the objective Attach the AZ AN DIA DIC Prism Prism holder or F Holder with Analyzer to the slot on the AZ100 and insert a DIC ooo the prism slider for the diascopic illumination into the slot on the illumination prism holder Select a DIC prism slider suitable to the objective in use Refer to the following table The second prism is inserted to the position
3. Dirt on the lens or filter adversely affects the viewing of the image If any of the lenses get dirty clean them as described below Remove dust particles with a soft brush or lightly wipe off with a gauze Only when fingerprints or oily substances are attached use soft clean lens tissue cotton cloth or gauze with a little absolute alcohol ethyl alcohol or methyl alcohol moistened to remove the dirt Do not use any solvents other than absolute alcohol as they may damage the lens adhesion surfaces Especially do not use petroleum benzine for the lens or filter Absolute alcohol is extremely flammable Keep these flammable solvents away from fire or sparks emitted when turning on off the power switch Use absolute alcohol according to the instructions provided by the manufacturer Cleaning the Product We recommend that you use a silicon cloth to clean the product For persistent dirt dampen a piece of gauze with neutral detergent and wipe lightly Use of organic solvent could result in discoloration of the plastic parts Decontamination We recommend that you use 7096 medical alcohol for decontamination of the product When a specimen is dropped onto the microscope check that the specimen is hazardous or not If the specimen is hazardous follow the standard procedure of your laboratory Use of organic solvent may result in discoloration of the plastic parts Storage of the Product Store the product
4. body with aligning the locating pin with the reference position Single with the reference and secure it by tightening four screws upward from the bottom noseplece 4 hole and secure the with the hexagonal wrench size 2 5 mm provided with the Se part with four screws zoon bony Single nosepiece installation Attaching the triple nosepiece When the objective needs to be changed attach the AZ NP3 Focusing mount adapter Triple Nosepiece to the lower surface of the focusing mount adapter posa 1 Rotate the triple nosepiece disc part to a click stop position Set to a click with the reference hole and secure the part with three screws near the center 2 Align the locating pin with the reference hole and stop position insert the protruding part of the triple nosepiece into 4 the hole on the bottom of the focusing mount adapter 3 Secure the nosepiece by tightening three clamp screws near the center of the triple nosepiece upward from the bottom with the hexagonal wrench size 4 mm provided with the zoom body The clamp screws do not drop off from the triple nosepiece q Triple nosepiece 9 Align the locating pin Triple nosepiece installation Installation of the Focusing Mount Adapter Attach the focusing mount adapter to the pillar of the episcopic Focusing mount adapter stand 1 Remove the two clamp screw covers on the right of Clamp screw the focusing mount adapter and sufficiently loosen the two clamp sc
5. Fluorescence Attachment In e AZ NCB NCB filter for Corxial Epi Illuminator C FID AZ ND128 FLDIC O a C F1115 Fiber Illuminator Birfurcated Fiber AZ DPS1 DIA DIC Prism Slider AZ AN DIA DIC 1 4x Prism Holder with SERO Analyzer AZ DPS5 mo DIA DIC Prism Slider 5x QG9 p AZ100 zoom body AZ EPS1 EPI DIC Prism Slider 1 4x RESO AZ PH DIA DIC AZ EPS5 Prism Holder EPIDIC Prism Slider 5x pL BED gm Oe DAD Mo LIE i STG S See hee AS oonan ane eee LED illuminator Nosepiece AZ LED RED Ring Fluorescence filter cubes AZ FLDIC FL DIC Prism Holder fa AZ FM AZ Focusing Mount Adapter AZ NPS Single Nosepiece AZ NP3 Triple Nosepiece Illuminator i Objective i f AZ Plan Apo AZ Plan Apo AZ Plan Fluor 0 5x 1x 2x i i AZ QLL AZ QLM 1 4 Lambda 1 4 Lambda Plate 0 5x Plate 1x AZ Plan Apo AZ Plan Fluor B AZ QLH 1 4 Lambda Plate 4 5x Illuminator SL AZ HGA HG Lamphouse C FC Epi Fl Adapter Collector Lens C SHG 100W cum mercury starter Mercury lamphouse 4BL mercury socket AZ HGFA Fel Fiber Adapter 9 C HGFI cm T g Intensilight C HGFIF15 HG fiber 1 5m C HGFIF30 HG fiber 3m f C HGFIE Intensilight motorized model o C HGFIE C HG controller Stand i 1 1 1 1 1 1 a DI
6. Illuminator as a light source e AZ FL Epi Fluorescence Attachment This illuminator is used for the epi fl microscopy It is attached in place of the focus mounting adapter and requires a mercury lamp light source Microscopy This chapter indicates the standard procedure for the microscopy with the AZ100 1 2 3 4 Set the optical path selector to the binocular part position Press the optical path selector lever to use the optical path of the binocular part Set the specimen on the stage Turn on the light source for the illuminator to be used and adjust its brightness Adjust the brightness properly with the brightness control knob on the light source and the ND filter Adjust the interpupillary distance of the binocular part Adjust the distance between the eyepieces to fit the distance between the user s eyes so that the image viewed through the eyepieces with both eyes coincides Adjust the diopters of the eyepieces Adjust the diopters of the right and left eyepieces to fit the user s eyesight 1 Loosen the clamp screws on the diopter adjustment rings on the right and left eyepieces and align the rings to the zero position Set the zoom magnification to 1x Focus on the specimen with the focus knob or the stage height adjustment knob Set the zoom magnification to 8x Focus on the specimen with the focus knob or the stage height adjustment knob again Set the zoom magnification to 1x o0 RF o N
7. at the illumination Set of the DIC Prism slider side below the specimen Select a DIC prism according to the magnification of the objective and insert it into the DIC slot on the AZ STD Diascopic Stand For details see the instruction manual for AZ STD Diascopic Stand the AZ STGD DIA Stage Selection of a DIC prism slider Each DIC prism slider has the same color code symbol of the corresponding objective Check the color code symbol on the objective to be used and use the DIC prism slider corresponding to the color code DIC prism slider SINE Colon oud for ae ucl uere for BOUES RUD STE 1x Gold AZ EPS1 AZ DPS1 4x Red AZ EPS1 AZ DPS1 5x Orange AZ EPS5 AZ DPS5 The DIC microscopy cannot be performed with the Plan Apo 0 5x or Plan Fluor 2x Operation of the DIC prism slider Loosen the clamp screw on the side of the prism holder or the analyzer for the diascopic illumination To place the DIC prism into the optical path push in the DIC prism slider as far as it goes To remove the DIC prism from the optical path pull the slider fully Note that if the clamp screw is loosened too much the DIC prism slider will be detached For the interference color adjustment place the DIC prism into the optical path and rotate the prism position knob at the edge of the slider to move the prism 15 Assembly A N Caution Be sure to read the Safety Precautions and follow the all instructions given there
8. base part the stage is located at the center of the elevating range Refer to the right figure Torque of the stage height adjustment knobs The torque adjustment ring is located on the left stage height adjustment knob The torque required to rotate the knob can be adjusted by rotating this ring The torque is increased by rotating the torque adjustment ring in the direction of arrow Adjust the torque enough to prevent the zoom body from descending with its own weight 12 Focus knobs both sides DE Coarse focus T knob Zoom E amp l 1 Fine focus knob body Torque T a Stage height adjustment ring Fine focus adjustment knobs knob both sides Coarse focus knob Zoom body height adjustment Mark on the stage elevating section Chapter 3 Operation Procedures Focus on the Specimen Continued Focusing Procedure Focus on the specimen according to the following procedures 1 Check the working distance of the objective The distance between the focal plane and the lower end of the objective is called working distance Focusing will be easier by adjusting the distance between the specimen and the objective considering the working distance Refer to Attaching An Objective s in page 20 for the working distance of each objective Focus on the specimen Rotate the focus knob on the zoom body to move the zoom body ve
9. holes refer to the right figure LIL ei ay fay l f 200 2 M6 depth12 Clamp screw hole dimensions Installation of the EPI Stage Attach the EPI stage to the substage of the episcopic stand 1 Remove the fastening hardware from the stage board The fastening hardware is secured with four hexagonal socket head screws Remove the screws with the 3 mm hexagonal wrench provided with the stage 2 Place the EPI stage on the substage and secure it by fixing the provided four M4 screws Secure the screws with the 3 mm hexagonal wrench provided with the stage For the installation of the AZ STGD DIA Stage see the instruction manual for the AZ STD Diascopic Stand and the AZ STGD DIA Stage EPI stage installation 18 Chapter 4 Aseembly Installation of the Single Triple Nosepiece The product can be used with the AZ NPS Single Nosepiece or AZ NP3 Triple Nosepiece to attach an objective objectives Before attaching the zoom body or the focusing mount adapter attach a nosepiece The single nosepiece is attached to the bottom of the zoom body The triple nosepiece is attached to the bottom of the focusing mount adapter Attaching the single nosepiece When only one objective is used and does not need to be changed attach the AZ NPS Single Nosepiece to the lower surface of the zoom body Attach the single nosepiece to the lower surface of the zoom Ali ae A MH sis ign the locating pin
10. microscope but contact your nearest Nikon representative Notes on handling a hazardous sample Check to determine whether the specimen is hazardous before handling If the specimen is hazardous follow your standard facility procedures Safety Precautions Notes on Handling the System 1 Carefully handle the product This product is a precision instrument Handle it carefully and do not subject it to damage or vibrations Especially the accuracy of the objective may be adversely affected by even a slight damage Cautions in carrying the product Securely hold the bottom front of the microscope base and indentations at the bottom back of the stage pillar when carrying the microscope e Do not grasp the stand pillar elevating section stage stage height adjustment knob focus knob eyepiece tube or zoom body when carrying the microscope to avoid breakage adverse effect on its performance or malfunctions 2 Installation or storage location The microscope is a precision optical instrument Using or storing the product under unsuitable conditions may damage it or may have an adverse effect on its performance The following conditions must be considered when selecting the installation or storage location e For installation select a location with a temperature from 0 to 40 C and a relative humidity of 85 96 or less no condensation For storage select a location with a temperature from 20 to 60 C and a relative humidi
11. the eyesight 1 Placea specimen on the stage 2 Loosen the clamp screw on the diopter adjustment ring and adjust the ring on the right and left eyepieces to the 0 zero position 3 Set the zoom knob to 1x 4 Focus on the specimen with the focus knobs and the stage height adjustment knobs 5 Set the zoom knob to 8x 6 Focus on the specimen again 7 Return the zoom knob to 1x 8 Clamp screws Diopter adjustment ring Diopter Adjustment Look though the right eyepiece with your right eye and the left eyepiece with your left eye and rotate the diopter adjustment ring of the eyepiece to focus on the specimen Adjust the right and left eyepieces respectively 9 Repeat step 5 and step 8 until the image stays in focus even though the objective magnification is changed 10 Secure the diopter adjustment ring by slightly tightening the clamp screw Chapter 3 Operation Procedures Operation of the Stage Place a Specimen Sufficiently lower the stage by rotating the stage height adjustment knob and place a specimen on the stage without being touched to the objective Stage Movement The EPI stage moves in two methods the coarse movement for rough positioning and fine movement for fine positioning For operation of the DIA stage see the instruction manual for the AZ STD Diascopic Stand and the AZ STGD DIA Stage How to perform the coarse positioning on the stage When the coa
12. A DIC AZ STGE EPI Stage AZ STGD Ed Stage AZ SG Stage Glass Cf ooo SMZ transparent glass AZ SRP AZ SDA Rotatable Plate Dish Holder Adapter TE TS series holder AZ STA Thermo Plate Adapter AZ series system configuration 17 AZ STE Episcopic Stand AZ STD Diascopic Stand AZ DP1 DIA Prisms EO 3 i AZ DP4 Prism pu Prismax BEEN AZ DP5 DIA Prismsx IEEE AZ RP Rotatable GEHES Polarizer AZ DL DIA DIC Lambda Plate EO AZ OI diagonal a illuminator slider Chapter 4 Aseembly Installation of the Episcopic Stand Install the episcopic stand on a level and stable surface Refer to page 3 Notes on Handling the System for installation location Removing cushioning materials The substage section is secured with cushioning materials Turn the stage height adjustment knob to raise the substage and remove the cushioning materials The AZ STD Diascopic Stand is required to use the diascopic illumination For the installation of the diascopic stand see the instruction manual for the AZ STD Diascopic Stand and the AZ STGD DIA Stage Remove the cushioning materials Removing cushioning materials Clamp screw holes ing eng The Episcopic stand has two clamp screw holes on the back They can be used for fixing the stand to a base or a pedestal e CD For the dimensions of the screw
13. D Focus on the specimen with the diopter adjustment rings on the eyepieces Look through the left eyepiece with your left eye and the right eyepiece with your right eye and adjust the right and left eyepieces individually 8 Repeat step 4 to step 7 until the image stays in focus even though the zoom magnification is changed 9 Lightly tighten the clamp screws to secure the right and left diopter adjustment rings Place an objective into the optical path when the triple nosepiece is used When the triple nosepiece is used objectives can be selected For the episcopic illumination adjust the aperture diaphragm For the diascopic illumination adjust the condenser the aperture diaphragm and the field diaphragm Adjust the brightness if necessary Focus on the specimen by adjusting the position of the specimen Adjust the position of the specimen with the stage movement knob until the observation point is located at the center of the viewfield e Focus on the specimen with the stage height adjustment knobs and the focus knobs Observe the specimen under a magnification adjusted by zoom operation The magnification can be changed with the zoom knob in the range as follows 1 multiplied by the magnification of the objective to 8 multiplied by the magnification of the objective Chapter 2 Microscopy 10 After observation turn off the light source After observation turn off the light source and the lamp If the m
14. M408 E ocizNr1 M403EN01 Nikon MULTIZOOM AZ100 MULTI PURPOSE ZOOM MICROSCOPE Instruction Manual Introduction Thank you for purchasing the Nikon products This instruction manual is written for the users of the Nikon MULTI PURPOSE ZOOM MICROSCOPE MULTIZOOM AZ100 To ensure correct usage read this manual carefully before operating the instrument e tis prohibited to reproduce or transmit this manual in part or whole without Nikon s expressed permission The contents of this manual are subject to change without notice Although every effort has been made to ensure the accuracy of this manual if you note any points that are unclear or incorrect contact your nearest Nikon representative e Some of the products described in this manual may not be included in the set you have purchased Also be sure to read the manuals for any other products that you are using with this system Not following the instructions specified by the manufacturer may adversely affect the safety of the production Safety Precautions To ensure correct and safe operation read this manual before using the product Warning Caution Symbols Used in This Manual Although Nikon products are designed to provide you with the utmost safety during use incorrect usage or disregard of the instructions can cause personal injury or property damage and will lead to the forfeiture of all claims against warranty For your safety read the instruction m
15. anual carefully and thoroughly before using the product Do not discard this manual but keep it near the product for easy reference In this manual safety instructions are indicated with the symbols shown below Be sure to follow the instructions indicated with these symbols Symbol Details 2 Disregarding instructions marked with this symbol may lead to death or serious Warning injury g Disregarding instructions accompanying this symbol may lead to injury or property Caution damage Intended application of this product This product should only be used for microscopic observation Do not use this product for other purpose Do not disassembly Disassembling may cause malfunction and will lead to the forfeiture of all claims against warranty Do not disassemble the section which is not indicated in this manual If you notice any abnormalities contact your nearest Nikon representative Read the instruction manual carefully To ensure safety carefully read this manual and the manuals for other equipment used with this product Especially be sure to follow the warnings and cautions indicated in the first of this manual A N Caution 1 Do not wet the microscope nor allow foreign matter to be intruded Do not wet the microscope Water splashed onto any component of this product may result in malfunction Also malfunctions may occur if foreign matters intrude If any liquid or foreign matters intrude do not use the
16. arse and fine knobs Objective both sides _EPI stage Triple nosepiece AZ STGE Single nosepiece AZ NP3 AZ NPS EPI stand AZ STE Light guide fiber provided with the AZ ICI Coarse movement lever Fiber Illuminator Coarse fine movement WESS selector switch C FI115D C FI115 C FI230 Stage height adjustment knob coarse and fine knobs both sides Stage movement knob X direction Y direction Configuration example The above figure shows the configuration example of the following parts AZ TE80 Ergonomic Trinocular Tube 80 AZ W10X Eyepiece 10X AZ100 Zoom Body AZ FM AZ Focusing Mount Adaptor objectives AZ NP3 Triple Nosepiece AZ STE Episcopic Stand AZ STGE EPI Stage AZ ICI Coaxial Episcopic Illuminator with a light guide fiber and the C FI115D C F1115 C F1230 Fiber Illuminator Use of the illuminator To perform the microscopy a specimen must be illuminated with a light source or an illuminator AZ LED LED Ring Illuminator This light source is attached to the tip of the objective e AZ ICI Coaxial Episcopic Illuminator This illuminator is used for the episcopic illumination It is attached to the portion between the zoom body and the eyepiece tube with the Fiber Illuminator as a light source Refer to the figure shown above e AZ STD Diascopic Stand and AZ STGD DIA Stage The stand and the stage are used for the diascopic illumination with the Fiber
17. axial XY knobs AZ NPS Number of attachable objectives One piece Single Nosepiece Objective attachment screw M38 x 1 25 AZ NP3 Triple Nosepiece Number of attachable objectives Three pieces Objective attachment screw M38 x 1 25 AZ TE80 Ergonomic Trinocular Tube 80 and AZ TE100 Ergonomic Trinocular Tube 100 Configuration Optical path selector Binocular part Vertical tube Magnification Field number Trinocular eyepiece tube erect image trinocular eyepiece tube and vertical tube Manual lever pressing pulling type Binocular part 100 Vertical tube 0 or Binocular part 20 Vertical tube 80 AZ TE80 Binocular part 100 Vertical tube 0 or Binocular part 0 Vertical tube 100 AZ TE100 Type Siedentopf Interpupillary adjustment 50 mm to 75 mm Diopter adjustment use the adjustment function of the eyepiece Able to attach the C mount adapter Binocular part 1x vertical tube 0 6x Binocular part 22 vertical tube 211 General specification With the following configuration AZ100 Zoom Body AZ STE Episcopic Stand AZ STGE EPI Stage AZ NPS Single Nosepiece AZ NP3 Triple Nosepiece AZ TE80 Ergonomic Trinocular Tube 80 External dimensions Weight Operation condition Storage condition 300 width x 427 depth x 637 height mm excluding protrusions such as those on the stage Approximately 20 kg Temperature 0 to 40 C Humidity 85 RH maximum no condensation Al
18. before assembling the product This chapter describes the procedure for assembling the AZ100 with the following parts AZ TE80 Ergonomic Trinocular Tube 80 AZ W10X Eyepiece 10X AZ100 Zoom Body AZ FM AZ Focusing Mount Adapter AZ NPS Single Nosepiece AZ STE Episcopic Stand and AZ STGE EPI Stage For the installation procedure of the Coaxial Episcopic Illuminator AZ ICI AZ STD Diascopic Stand AZ STGD DIA Stage AZ FL Epi Fluorescence Attachment AZ LED LED Ring Illuminator refer to their instruction manuals Necessary tools The following tools are required to assembly the product Tool Appearance Remark Hexagonal screw driver CEEEEMeS Provided with the AZ100 zoom body size 2 mm Hexagonal wrench f size 2 5 mm Provided with the AZ100 zoom body Hexagonal wrench size 3 mm Provided with the EPI stage Hexagonal wrench Provided with the AZ100 zoom body size 4 mm Y 16 Chapter 4 Aseembly System Configuration The AZ series system configuration is shown below AZ W10X igital camera for microscope DXM1200 Series Digital Sight Series C mount direct TV adapter YM ND 25 pacco 0 see ee Ees E M ND4 ND16 i TE10I Eyepiece tube Ergonomic AZ TEB0 mm peee g Trinocular Ergonomic DSC Tube Tube 100 Trinocular Tube 80 AZ EL EPI DIC Lambda plate 0 6x AZ ICI AZ FL Epi
19. d eet eee t eleme det ede ea dese dote o UI ee 11 Selection of an Objective for the Triple Nosepiece ssseeH mH 11 FOCUS On the Speclmer z uu trt t RR ra EM U EA 12 Operation of the Aperture Diaphragm ssssssssssseeneeenenmenee eene nnne 14 Setor the DIC Prism Slider re Re Ret e td E e c ecu 15 Chapter 4 Assembly 5 e nne eer aia aieiai raaraa ad reaa aaee Soina a ede aed naso rev ena edt 16 System Configuration dice ers de cue eue de uu e e uere Peg ae dede ace e be edo beu adds 17 Installation of the Episcopic Stand eene nnne 18 Installation of the EPI Stage ai eee erates Aiea ee tie nse 18 Installation of the Single Triple Nosepiece sssssssssseeeenem eene 19 Installation of the Focusing Mount Adapter sssseseeee emen nnns 19 Installation of the Zoom BOQY os eanes ieina i aaia aeria enemies 20 Attaching an ObJectlve s 4 rte e o it Lett eB ects Ebo eet re EEEE 20 Installation of the Eyepiece TUDO er aaeain anaana EAE AE EEEE EA EAEE ERR en neret nennen nnns 21 Attaching Eyepieces indeed elaine leit 21 Attaching the Photographic Device Option emen 21 Chapter 5 Troubleshooting i eden eae din aaan anapa CREE LL seman e CE AVR ead eee 22 Troubleshooting on Image Viewing ssssesseeee enm emm eene 22 Troubleshooting ori Operatlot 2 rii ete en ed bte n ede atocatiebtecetiass 23 Chapter 6 Care and Mai
20. ening size of the aperture diaphragm is too small Increase in the opening size appropriately p 14 An unnecessary filter is in the optical path Set only a necessary filter into the optical path Dirt or dust is seen in the field of view Dirt or dust is attached to a lens objective and eyepiece Clean the lenses p 24 Dirt or dust is attached to the specimen Clean the specimen The opening size of the aperture diaphragm is too small Increase in the opening size appropriately p 14 The image is partially dim The stage is inclined Attach the stage correctly p 18 The image moves during operation The specimen is inclined with respect to the stage surface Place the specimen on the stage correctly p 10 The stage is inclined Attach the stage correctly p 18 22 Chapter 5 Troubleshooting Troubleshooting on Operation Trouble Cause Countermeasure The image is not in focus although the zoom body is moved to the lowest position The position of the specimen is extremely low Adjust the position of the specimen with the stage height adjustment knob p 12 The image is not in focus although the stage is moved to the higher limit position The zoom body is raised too high Adjust the position of the zoom body with the focus knob p 12 After changing the zoom magnification the specimen goes out of focus
21. fication zoom magnification x objective magnification x eyepiece magnification When the vertical tube is used change the eyepiece magnification to the vertical tube magnification 0 6x to calculate the total magnification The magnification of the AZ ICI Coaxial Episcopic Illuminator is 1 25x Therefore when the AZ ICI is used the total magnification is obtained by multiplying the above formula by 1 25 Zoom knob click ON OFF When the click ON OFF lever on the bottom of the right zoom knob is flicked to the ON side the click stop positions on the zoom knob are provided at the integral multiples of the zoom magnification 2x 3x 4x 5x 6x and 7x de LR Bl Zoom knob 4 4 0 52 _ A Click ON OFF i5 Ba lever a Zoom operation Selection of an Objective for the Triple Nosepiece When the AZ NP3 Triple Nosepiece is used it can be attached with up to three objectives To select an objective rotate the nosepiece to a click stop position manually 11 Hu a A C Las gt L Rotate tne Se part manually Triple nosepiece Selection of an objective Chapter 3 Operation Procedures Focus on the Specimen A N Caution To avoid malfunctions do not rotate the right and left focus knobs and the right and left stage height adjustment knobs in an opposite direction Additionally do not rotate those knobs excessively Do not rotate the fine f
22. further in the same direction after it comes in contact with the limited position to avoid malfunctions 5 Cover the vertical tube The eyepiece tube is equipped with the vertical tube To keep out ambient light and dust always attach the provided cap to the vertical tube that is not currently in use Contents nigere l titor eL 1 KE eT CE 2 Warning Caution Symbols Used in This Manual ssee enne 2 Notes om Handling tlie System iio ettet eoe tege stet edie eei bt Lee eet tung edens 3 Chapter 1 Part NAMOS a quatn cem dete erbe maa gic eod Rabe Deva evens 5 Chapter 2 UC eT pec 6 Chater 3 Operation Procedures cc ecccceeceeeeeee teen eeee seen nena cane ceee eee ena nnne nnnn nennen nnn nett entr nen n nnn 8 Operation of the Eyepiece Tube Part sssssssssssssssseseeeneeme enne enne n enne 8 Change of the Optical Path 1 imei e cut t eU e a Lg eR does 8 Angle Adjustment of the Binocular Part Angle 8 Adjustment of the Interpupillary Distance seeeennn menn 9 Adjustment of the DIODpters ce acute nea enean o nee entr e Ue e ee Rp tnde 9 Operation of the Stage tede itn deinen dtd de a qe Eaa reisai dt e ede gei 10 Place a SpecImeris uen tn tenian tun ai tee eia 10 Stage MOVGITielt s teeth ecteteest os etate iot on ete nte Dp d ten bt D peut toa eto ees 10 Zoom Operat O ser cee ie de
23. h the lever pull the lever Binocular part 100 Binocular part 20 RE TESS Vertical tube 0 Vertical tube 80 AZ TE100 Binocular part 100 Binocular part 0 Vertical tube 0 Vertical tube 100 Change of the optical path Angle Adjustment of the Binocular Part Angle The binocular part of the ergonomic eyepiece tube can be elevated in the range from 0 horizontal direction to 30 degrees Adjust the angle so that observation can be easily performed For the angle adjustment of the binocular part operate the binocular part with holding it with both hands At this time be careful not to pinch your hand or finger Horizontal direction Adjustment of the binocular part angle Chapter 3 Operation Procedures Adjustment of the Interpupillary Distance Adjust the distance between the eyepieces according to the interpupillary distance so that the images viewed through the eyepieces with both eyes coincide The binocular eyepiece has a scale for interpupillary distance It is recommended to memorize or record your interpupillary distance so that the interpupillary adjustment can be performed easily next time CORO Viewfields coincide For the interpupillary adjustment operate the binocular part with holding it by both hands and be careful not to pinch your fingers Adjustment of the Diopters Interpupillary adjustment Adjust the eyepiece diopters according to
24. icroscope or the light source is to be covered by a dust cover cool down the light source sufficiently Bright field microscopy under epi illumination 4 Adjust the interpupillary distance of the binocular part 9 Observe the specimen under a magnification adjusted by zoom operation 5 Adjust the diopters of the eyepieces Set the optical path selector 1 to the binocular part position T Episcopic illumination Adjust the aperture diaphragm Diascopic illumination Adjust the condenser the aperture diaphragm and the field diaphragm 6 Place an objective into the optical path 2 Setthe specimen 3 Turn on the light source and adjust the brightness 10 After observation turn off the light source Adjust the position 8 of the specimen and then focus on the specimen 3 Operation Procedures This chapter indicates the operation procedures of each component of the AZ100 Operation of the Eyepiece Tube Part Change of the Optical Path When the AZ TE80 Ergonomic Trinocular Tube 80 or the AZ TE100 Ergonomic Trinocular Tube 100 is used the optical E path of the eyepiece tube can be changed with the optical path lt Optical path selector lever QE 7 selector lever The light distribution between the binocular part and vertical n Joss N tube can be changed by pushing or pulling the lever C t Optical path BINO PHOTO E selector lever pus
25. in a dry place where mold hardly grow We especially recommend that the objectives and eyepieces be stored in a container such as a desiccator with desiccant in it Cover the product with a dust protection cover when storing so that it will not get dusty Cover a dust protection cover after turning off the power source press the front half of the power switch and the lamp cools sufficiently Regular Inspections With Charge Regular inspection with charge is recommended to maintain the performance of the product Contact your nearest Nikon representative for details 24 F Specifications AZ100 Magnification 1x to 8x zoom ratio 8 1 Zoom Body Aperture diaphragm 6 mm to 20 mm Zoom knobs Right and left knobs are connected with the clickstop positions at integral magnifications Slot DIC prism slot AZ STE Substage Stroke in the vertical direction 10 mm Episcopic Stand Coaxial coarse fine focus knobs torque is adjustable Coarse focus 37 7 mm per cycle Fine focus 0 27 mm per cycle Maximum weight of the specimen 1 kg for the AZ STGE EPI stage Focus mounting section Stroke in the vertical direction 85 mm Coaxial coarse fine focus knobs torque is adjustable Coarse focus 18 5 mm per cycle Fine focus 3 27 mm per cycle AZ STGE Stroke 150 mm x 150 mm EPI Stage Stage movement Coarse focus with the coarse fine focus selector switch Fine focus vertically arranged co
26. ntenance 2ccccceeeeeeeeeeeeeeeeee eeeeeeeeeeeeeeee ene eee sane cess sees enhn tnter enne s nnn nnn 24 Cleaning the Lenses oe ern ata enema 24 Cleaning the Product b trt edv d tta tte de ads aerate 24 IDecontarmiriation oen o Ir PEERS A ete de ebd OR te nag ses OA Deas doro ae piet I 24 Storage of the Product gcc eee ieu ee dede ee ee aeu aede 24 Regular Inspections With Charge sse AARE EEA nenne 24 Chapter 7 Specifications ioi ren tenen P pe rotae eee pea EOD eue ended eee 25 Part Names The Nikon MULTI PURPOSE ZOOM MICROSCOPE MULTIZOOM AZ100 is the macro mono zoom microscope that is suitable for observation or photomicrography in the range from macro to medium magnification The microscope can use five types of objectives 0 5x 1x 2x 4x and 5x objectives The product can zoom in or out in the magnification range of 1x to 8x for each objective The total magnification range is 0 5x to 40x Binocular part _ Vertical tube i w Sa Ergonomic trinocular tube Eyepiece E Ps AZ TE80 AZ TE100 AZ W10X N 4 Optical path selector lever Diopter adjustment ring ____ Focusing mount adapter AZ FM Coaxial episcopic illuminator AZ ICI Elevating section Zoom knobs both sides CLICK ON OFF lever Zoom body lens barrel AZ100 Slider slot Aperture diaphragm open close dial Focus knobs co
27. ocus knob with holding the coarse focus knob nor vice versa On this product two types of height adjustment can be performed the stage height adjustment and the zoom body height adjustment Use any appropriate knob for your convenient Zoom body height adjustment The focus knobs are located on the both sides of the stand elevating section These knobs are used to adjust the height of the zoom body to focus on the specimen The coarse focus knob and fine focus knob are provided for this purpose One revolution of the coarse focus knob allows the stage to move by 18 5 mm and that of the fine focus knob allows by 3 27 mm Torque of the focus knob The torque adjustment ring is located inside the left focus knob Insert the hexagonal screwdriver into the hole on the ring and adjust the torque enough to prevent the zoom body from descending with its own weight The torque is increased by rotating the torque adjustment ring upward and decreased by rotating the ring downward Stage height adjustment The stage height adjustment knobs are located on the both sides of the stand elevating section The coarse focus knob and fine focus knob are provided One revolution of the coarse focus knob allows the stage to move by 37 7 mm and that of the fine focus knob allows by 0 27 mm The center of the elevating range reference position is marked on the right elevating section of the stage When the mark is located at the height of the microscope
28. ore this step Zoom body installation Attaching an Objective s Screw an objective objectives into the single or triple nosepiece Raise the elevating section of the stand and lower the stage And then screw the objective into the attachment hole Be careful to avoid bumping the objective The objectives below are applicable i Screw the Objective Magnification Actes Color code objective into the attachment hole AZ Plan Apo 0 5x 0 5 54 Silver AZ Plan Apo 1x 1 35 Gold Objective AZ Plan Fluor 2x 2 45 White Attaching an objective AZ Plan Apo 4x 4 20 Red AZ Plan Fluor 5x 5 15 Orange The color codes are used to select the DIC slider for the DIC microscopy or to select the condenser or the filters for the diascopic stand 20 Attach the adapter dovetail groove with the dovetail joint Chapter 4 Aseembly Installation of the Eyepiece Tube Attach the eyepiece tube to the upper surface of the zoom body The AZ TE80 Ergonomic Trinocular Tube the AZ TE100 Ergonomic Trinocular tube 100 or the photo adapter for the AZ TP series are available for the zoom body 1 Sufficiently loosen the eyepiece tube clamp screw on the right of the zoom body with the provided hexagonal wrench 2 Align the circular dovetail joint on the lower part of the eyepiece tube with the mounting location of the zoom body with inclining the eyepiece tube to the left backward and attach the eyepiece tube
29. rews with the hexagonal wrench size 4mm provided with the product Attach the adapter with aligning the dovetail groove with the dovetail joint 2 Align the dovetail groove of the focusing mount e adapter with the dovetail joint of the episcopic Dovetail joint stand pillar and slide down the focusing mount adapter to be attached Be sure to slide down the focusing mount adapter as far as it goes Tigh h I h For the triple nosepiece configuration 3 Ig ten the two clamp screws to secure the attach the nosepiece to the focusing mount focusing mount adapter and attach the clamp screw adapter before this step covers Focusing mount adapter installation 19 Chapter 4 Aseembly Installation of the Zoom Body Attach the AZ100 zoom body to the focusing mount adapter Zoom body and single nosepiece 1 Remove the two clamp screw covers on the right of the zoom body and sufficiently loosen the two clamp screws with the hexagonal wrench size 4mm provided with the product Clamp screw with aligning the 2 Align the dovetail groove of the zoom body with the dovetail joint of the focusing mount adapter and slide down the zoom body Dovetail joint Be sure to slide down the zoom body as far as it goes 3 Tighten the two clamp screws to secure the zoom Focusing mount body and attach the clamp screw covers adapter For the single nosepiece configuration attach the nosepiece to the zoom body bef
30. rse fine movement selector switch of the EPI stage is pulled toward the coarse movement lever the stage moves coarsely in the X axis and Y axis directions Therefore hold the coarse fine movement selector switch and the coarse movement lever together and move the stage Stage 5 Coarse fine movement selector switch If the stage is moved only with the coarse movement lever without holding the coarse fine movement selector switch or if the stage is moved directly with holding the top plate of the stage a malfunction occurs Be sure to hold the coarse fine oarse movement movement selector switch together for the stage movement lever Stage fine movement knob How to perform the fine positioning on the stage Stage movement If the coarse fine movement selector switch is not held the stage is in the fine movement mode so that the specimen can be positioned by degrees with the stage fine movement knobs The stage is moved in the Y axis direction with the upper knob and in the X axis direction with the lower knob 10 Chapter 3 Operation Procedures Zoom Operation The zoom knobs are located on the both sides of the zoom body Rotation of these knobs allows the zoom magnification to be changed in the range of 1x to 8x continuously Total magnification The zoom magnification is indicated on the left Zoom knob The microscope total magnification is determined with the following formula Total magni
31. rtically and focus on the specimen Insert the hexagonal screwdriver into the hole on the ring and adjust the torque enough to prevent the zoom body from descending with its own weight Operate the coarse focus knob for the coarse adjustment and the fine focus knob for the fine adjustment Especially the fine focus knob is useful for focusing on the specimen at a high magnification 13 Chapter 3 Operation Procedures Operation of the Aperture Diaphragm The aperture diaphragm open close dial is located on the front of the zoom body The aperture diaphragm controls the numerical aperture of the illumination system closely related to the optical image brightness contrast resolution and depth of focus Qd The aperture diaphragm of the zoom body is adjusted with the C 3 aperture diaphragm open close dial and the image changes in F response to the adjustment as below Aperture diaphragm Close amp Open x Brightness Dark Bright Aperture diaphragm if open close dial Depth of focus Great Shallow The more the aperture diaphragm is closed the greater the depth of focus becomes but the resolution deteriorates Under a low magnification effect of the aperture diaphragm operation is smaller Aperture diaphragm operation 14 Chapter 3 Operation Procedures Set of the DIC Prism Slider For the DIC prism slider installation the zoom body is provided with a slot for it
32. t with the clamp screw Mounting location Eyepiece tube clamp screw Eyepiece tube installation Attaching eyepieces Attach the adapter to the Photographic ex DS Fit device LJ C mount direct 4 TV adapter Insert the adapter as far as it goes and secure it with a clamp screw Attaching the Photographic device Troubleshooting Misuse of the product although it does not cause a malfunction may adversely affect performance If any of the following troubles come be sure to check the following table for possible causes before requesting service If the troubles cannot be resolved by taking the following measures contact your nearest Nikon representative Troubleshooting on Image Viewing Trouble Cause Countermeasure The field of view is vignetted not visible or uneven in brightness Or the image is too dark The product is not installed properly Install the product correctly p 16 to 21 The optical path selector lever on the eyepiece tube is located in an intermediate position Adjust the lever correctly p 8 The nosepiece is not rotated to the clickstop position For triple nosepiece Rotate the nosepiece to the clickstop position and move the objective into the optical path p 11 Dirt or dust is attached to the lens objective and eyepiece or the specimen Clean the lenses and specimen p 24 The op
33. titude 2000 m maximum Pollution degree Degree 2 Installation category Category II Temperature 20 to 60 C Humidity 90 RH maximum no condensation 25
34. to the zoom body Check that the eyepiece tube is attached horizontally 3 Tighten the eyepiece tube clamp screw to secure the eyepiece tube Attaching Eyepieces Attach the eyepieces to the binocular part of the eyepiece tube Insert the eyepieces as far as they go Firmly hold the rubber cover base when inserting an eyepiece Insertion with grasping the diopter adjustment ring may result in malfunctions Attaching the Photographic Device Option Use the optional C mount direct TV adapter to attach a photographic device such as a digital camera for microscope to the vertical tube of the eyepiece tube Attach the photographic device according to the following procedure 1 Attach the C mount direct TV adapter to the photographic device such as a digital camera for microscope For details on installation see the instruction manual for the photographic device 2 Sufficiently loosen the clamp screw on the vertical tube with the provided hexagonal wrench 3 Remove the protective cap from the vertical tube 4 Insert the C mount direct TV adapter to the vertical tube as far as it goes 5 Tighten the clamp screw on the vertical tube and secure the adapter A C mount adapter embedding a lens such as 0 7x or 0 55x cannot be used for the product 21 Photographic device Eyepiece tube j L Place the tube on the mounting location with aligning the circular dovetail joint and secure i
35. ty of 90 or less no condensation If the product is installed or stored in a location subject to high temperatures and humidity mold or condensation may form on the lens resulting in lowered performance and possible damage to the microscope Avoid installing in a place exposed to direct sunlight or directly under room lights or any other bright place The image quality deteriorates in bright surroundings because of the extraneous light entering the objective Install in a place with little dust and dirt e Install in a place with little vibrations Install and store the product on a sturdy desk or table that can bear the weight of the product Install the product in the location of 10 cm or more away from the surrounding walls Donotinstall in the narrow space such as shelf or locker e Do not place anything on the product e Cover the product to avoid dust when storing 3 Notes on handling optical parts Scratch or dirt such as fingerprints on the optical parts such as lens or filter affects the viewing of the image Carefully use the optical parts without scratching If any dirt attaches to the product clean it according to Chapter 6 Care and Maintenance 4 Handling the stage height adjustment knob and focus knob Donotrotate the right and left stage height adjustment knobs or the focus knobs in opposite directions to avoid damage Do not rotate those knobs in such a way Donotrotate the coarse focus knob
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