MultiGene Thermal Cycler Operation Manual
Contents
1. Key1 1 4 Maximum 12 characters re JE rs rs 23 8 Deleting a Protocol 1 An existing Protocol can be deleted under a User s Folder 2 Under the User s Folder screen choose a Protocol Select F4 DELETE to delete the selected Protocol 3 If F4 DELETE is selected the screen will prompt you Are you sure you want to delete Press F1 YES to confirm the deletion Press F5 NO to abort the deletion IKE gt PROTOCOL 8 21 2006 11 00 PROTOCOL DATE TIME Touchdown 07 25 06 09 10 20 Lab 07 28 06 03 25 30 Are you sure you want to delete Note Protocols in the Preset Protocols Folder can t be deleted while Protocols in the Public Folder can be deleted by any user if not password protected 24 MultiGene Thermal Cycler 9 System Setup System Setup contains 7 functions 25 1 2 3 Data Log contains the history of the latest run Protocol Date amp Time Users can change the Date and Time settings Lid Temperature Users can choose the preferred lid temperature as default setting during running a Protocol Beeper Users can turn on off the audible beep Administrator Users can delete unwanted Usernames and their Protocols Service Mode is reserved for Service engineers SYSTEM 8 21 2006 11 00 9 Date amp lime Lid Temperature Beeper Administrator Service Mode Press Enter or Fl to select2. 2 Temp 1 Temp 94 0 94 0 55 0 72 0 72 00 4 00 Time 5 00 0 30 0 30 0 30 10 00 o Mode 1 1 2 Volume Efjui 5 100u1 Run started 08 21 06 12 20 50 Run will end 08 21 06 13 50 20 J re J Frs J rs 5 4 Terminating a Protocol 1 Press FS EXIT or STOP key in the bottom left side of the Alphanumeric keypad to terminate a running protocol 2 The display will show message Do you want to abort as below 3 Press F1 YES or STOP key again to terminate the Protocol Press F5 NO to return to the running Protocol 4 The display screen will return to Protocol screen Mike gt Lab gt RUN gt START 8 21 2006 11 00 1 Temp 3Temp 25 2 Temp 1 Temp 94 0 94 0 55 0 72 0 72 00 Time 5 00 0 30 0 30 0 30 10 00 Estimated Remaining 01 20 YES Do you want to abort 17 6 Creating a New Protocol A new Protocol can be created by going to the User Folder and selecting and editing a basic Protocol format and then assigning it a new name 1 Select User name on User screen Press 1 or ENTER button to enter User Folder Key in PIN to enter the User s folder 3 Press F2 NEW to create a new Protocol by using a standard template shown below Note Users can create a new Protocol under Public folder without a PIN Users can create a new Protocol inside Preset folder BUT can not sav
3. and 100 115 are available SYSTEM gt LID TEMPERATURE 2006 8 21 11 00 Lid Temperature Function 1 OFF Lid Temperature 60 65 100 115 Press Fl to save your settings ACCEPT 27 9 4 Beeper Users can turn ON or OFF the sound of this unit by selecting the Beeper screen as shown below under SYSTEM screen Please remember to press FI ACCEPT to store your settings SYSTEM gt BEEPER 8 21 2006 11 00 Beeper Function E er 1 Press Fl to save your settings EE 9 5 Administrator The password of Administrator is 9600 The Administrator of this unit has rights to delete any User Folder and any Protocols inside a User s Folder 9 6 Service Mode Only authorized service personnel have the password to enter Service Mode and perform necessary maintenance and repairs 28 MultiGene Thermal Cycler 10 Maintenance and Troubleshooting 10 1 Cleaning the unit Please ensure that no liquid is spilled onto or into the unit In addition periodically wipe it clean of dust and other residue that comes with normal operation of the unit Use a soft lint free cloth and deionized water 10 2 Cleaning the heated lid Make sure the MultiGene is unplugged and cooled down To clean the heated slide the lid back on the tracks until the front black pins align with the two slots taking care that
4. operation 3 Press F1 START to run the Protocol Note 1 The highlighted temperature Temp value represents the current block temperature during Protocol running The highlighted time Time value below represents the remaining running time in the temperature segment Once the countdown is completed the highlighted section will continue to move to the next temperature or cycle segment and start counting down the time The Lid temperature will flash on the status bar when the Protocol starts and remain on until it reaches 50 C When the lid reaches 50 C HOT will appear in the right hand corner and remain until the temperature falls below 40 C The sample block will start to heat up when the lid temperature reaches its set temperature 5 2 Pause a Protocol During running of the Protocol press F1 PAUSE will pause the running Protocol for 10 minutes The Protocol will continue after the Pause stops The Protocol won t be paused until set temperature is achieved To resume running the Protocol press FI RESUME 5 3 Viewing Running Protocol Information Press F2 INFO to view the Protocol information screen as shown below during operation Press FS RETURN to return the running Protocol screen The Protocol information screen will return to the running Protocol screen in 3 minutes if F5 RETURN is not pressed 16 MultiGene Thermal Cycler Lab gt RUN gt START 8 21 2006 13 00 1 Temp 3 Temp 25
5. 4 30000000 7 35 eoi ett EE EPOR eet eir ter boe wa RA 10 3 6 KEY ENTRY SYSTEM e ete once botes ee ede EE ences 11 3 7 LOADING THE REACTION 5 11 4 USER FOLDER 2 2 2 2 6 6 12 4 1 VIEWING A USER FOLDER 20 240000 0000000 12 4 2 CREATING A NEW USER FOLDER eeen 13 4 3 EDITING USER FOLDER 1 0211 S 13 4 4 DELETING A USER FOLDER cccccccesescececccccccssssesceccesscsseusucsesccsssssseuscsaeesesseseseneneess 14 5 PROTOCOL MANAGEMENT cccccscscssssssscssssssscssssssssssssssssssscccccccccssccesscssssess 15 5 1 RUNNING A 00 0 0 0 00060 15 5 22 PAUSE A PROTOCOL itte tats eoo tete et oe eet ee tetti ti na 16 5 3 VIEWING RUNNING PROTOCOL 00 00 1 6 16 5 4 TERMINATING A PROTOCOL eene 17 6 CREATING NEW PROTOCOL cccccccccsccsssssssssccccccccccccccccsccccccccscscccssssssees 18 6 1 CHANGING TEMPERATURE TIME 6 0 18 6 2 INSERTING DELETING TEMPERA
6. 9 Temperature Accuracy 0 5 C 30 to 99 9 C 2 C 4 to 15 C 0 5 C 30 to 99 9 C 2 C 4 to 15 C Temperature 0 5 C 0 5 C Uniformity Max Temp 3 C 2 C 3 C 2 C Increment Decrement Rate Gradient Temperature 30 99 C N A 30 99 C N A Max temp Max temp difference 24 C difference 24 C Max Lid Temperature 100 115 C 100 115 C Protocol Capacity 100 sets 100 sets Operating Temperature 15 30 C 15 30 C Operating Humidity 20 80 RH 20 80 RH Networking 1 x RS 232 1x USB 1 x RS 232 1 x USB Power Supply 100 to 240V AC 50 60 Hz 100 to 240V AC 50 60 Hz 500VA 500VA Dimensions WxDxH 24 x 25 x 42cm 24 x 25 x 42cm Weight Net 9 Kg 9 Kg 32 MultiGene Thermal Cycler Appendix B Protocols in Default Folder e 2 Step PRESET gt 2 STEP gt RUN 8 21 2006 11 00 1 Temp 2 Temp 25 2 Temp 1 Temp 94 0 94 0 55 0 55 00 4 00 Time 5 00 0 30 0 30 10 00 Mode 1 1 2 Volume ul 5 100u1 1 Tube Control 2 Block Control START e Gradient PRESET gt GRADIENT gt RUN 8 21 2006 11 00 1 Temp 3 Temp 25 Cyc 2 Temp 1 Temp 940 940 550 720 720 40 Time 5 00 0 30 0 30 0 30 10 00 Mode 1 1 2 Volume ful 5 100u1 1 Tube Control 2 Block Control START 33 Touch down PRESET gt
7. Touch do gt RUN 2005 8 21 13 00 1 Temp 3 Temp 20 2 Temp Temp 95 0 95 0 ENSE 720 720 4 0 Time 5 00 0 30 0 30 0 30 10 00 Mode 1 1 2 Volume ul 5 100u1 1 Tube Control 2 Block Control START Time Increment PRESET gt Time inc gt RUN 2005 8 21 13 00 1 Temp 3 Temp 20 2 Temp Temp 95 0 95 0 72 0 72 0 4 0 Time 5 00 0 30 0 30 0 30 10 00 Mode 1 1 2 Volume ul 5 100u1 1 Tube Control 2 Block Control START 34 MultiGene Thermal Cycler Appendix C CE Declaration 35 Labnet International Inc 3 Mayfield Avenue Edison NJ 08837 U S A Declaration of Conformity Product Name MultiGene Gradient Non Gradient Thermal Cycler Model Names TC9600 G 9600 TC6000 G TC6000 All models comply with the following European standards EMC EN 61326 Group 1 Class B Safety 61010 1 and EN 61010 2 010 To the best of my knowledge and belief these units conform to these standards Name James McGuffick Position Quality Assurance Manager Issue Date 2006 10 4 Appendix D Hyper Terminal and USB Driver Setup Users are required to purchase the USB or RS 232 cable to connect the MultiGene and the PC For USB connection users are required to install the USB driver Hyper Terminal Setup l Qo ON a Click on the start tab of Windows and go to accessories Click the accessory tab Go to C
8. by 5 seconds the second step would be 55 C and a dwell time of 35 seconds 1 Place the cursor at the Temperature or Time values Press F3 INCRE 2 To select increment decremental values The screen will ask to input the increment values Press F3 or F4 to choose increment or decrement 3 Enter your desired values and press ENTER The graph will show the increment decrement values 4 Press F1 ACCEPT to accept the values or F2 CLEAR to re enter the values To remove the Incremental Decremental Temperature and Time settings place the cursor in the temperature segment with Incremental Decremental Temperature and Time values Select F2 CLEAR to clear the values Select Fl ACCEPT will remove the incremental Decremental Temperature setting and its symbol 21 Temperature Increment BBB gt PROTOCOL gt NEW gt INCRE 2005 8 21 13 00 1 Temp 3 Temp 25 2 Temp Temp 94 0 94 0 72 0 72 0 4 0 Time 5 00 0 30 0 30 0 30 10 00 Temperature Increment 0 0 ea m9 E23 ee 06533 Ig Max Temperature incremental value 9 9 C Max Time incremental value minute second 9 59 Note 1 Temperature limits 4 0 C to 99 0 C DO NOT exceed 2 Only Multi cycle segments will have incremental decremental time or temperature function Time Increment BBB gt NEW gt ADV 2005 8 21 13 00 1 Temp 3 Temp 25 2 Temp Temp 94 0 94 0 55 0 72 0
9. first Temperature position and pressing ENTER When the Temperature Segment Count is larger than one the temperature Cycle Count Cyc will appear The Multi Temperature Segment will cycle during this segment The maximum temperature segment per protocol is 12 segments The temperature segment count and cycle count ranges are shown below Temperature Segment Count Temp range 1 to 9 Cycle Count Cyc range 1 to 99 6 4 Advanced Settings Users can set up the following advanced functions 1 Gradient 2 Temperature Time Increment Decrement 3 Pause F4 ADVANCE button is used to enter these settings The screen below will be displayed 19 Mi ke gt PROTOCOL gt NEW gt ADV 8 21 2006 11 00 1 Temp 3Temp 25 2 Temp Temp 94 0 94 0 72 0 720 4 0 Time 5 00 0 30 0 30 0 30 10 00 Enter desired temp time or number RENS RS 6 4 1 Gradient Temperature Setting 1 To enter a Gradient Temperature setting place your cursor on the desired Temperature Segment Select F2 GRAD to enter the screen below 2 If Gradient is off Enter the Low temperature setting and the High temperature setting then press ENTER to confirm the values The screen will show the calculated temperature gradient from row 1 Left to I2 Right This reflects the variation of the temperature from the left side coolest to the right side hottest of the block 3 Press F1 ACCEPT to confirm th
10. o oor ed o ae eee el eee nao ooo 30 TI PSE RROR MESSAGES ass s lsa oie tsi Cete t pte t t tee t o I Dese ak ees 31 APPENDIX A TECHNICAL 8 2 4 2 12 32 APPENDIX B PROTOCOLS IN DEFAULT FOLDER 0 02 2 22 22 4 33 APPENDIX C CE DECLARATION 2 2 6 35 APPENDIX D HYPER TERMINAL AND USB DRIVER 36 APPENDIX E MULTIGENE RECOMMENDED CONSUMABLES 37 MultiGene Thermal Cycler 1 Safety Precautions Before using the MultiGene Gradient thermal cycler for the first time please read this entire operating manual carefully To guarantee problem free safe operation of the MultiGene thermal cycler it is essential to observe the following points 1 p 254 10 11 12 Do not use the machine in a potentially explosive environment or with potentially explosive chemicals Install the machine in location free of excessive dust Avoid placing the machine in direct sunlight Choose a flat stable surface capable of withstanding the weight of the machine Install the machine in the room temperature 15 30 C relative humidity 20 80 DO NOT block the air vents Keep the side and rear of the machine at least 30cm from the wall or other machine Make sure the power
11. the rolleiflex cable is clear Lift the lid from the front be careful of sharp edges and flip up Use mild detergents to clean material from the lid A kimwipe dipped in 70 ethanol will help remove and residue from sealing tape Make sure the lid is dry prior to lowering it into the tracks Once the lid in secure in the tracks make sure the lid easily slides forward 10 3 Replacing a Fuse The fuse is located in the power socket module just below the power connector If the unit does not turn on when the power switch is turned on then replace the fuse To replace the fuse 1 Disconnect the power cord from the unit 2 Remove the fuse drawer with a small blade screwdriver 3 Pull the fuse out of the fuse socket and replace the fuses with one having the correct current rating For 100V 120V operation Use 7 0A 5 x 20mm Glass Tube 5 For 220 V 240 V operation Use 3 15A 5 x 20 mm Glass Tube Reinsert fuse into the fuse socket Reinsert the fuse drawer 10 4 Changing the heater block Slide the lid to the furthest point back 2 Remove the two plastic plugs and loosen and remove the tow screws holding the face plate 3 Flip the face plate forward to gain access to the block Remove the two set screws holding the heating block 29 59 091 ON n Slide the new block into place and connect the plug Replace the two screws and plugs Shut the face panel and secure it with two screws 11 Troubleshooting
12. your System SELECT 9 1 Data Log The Data Log records a log of activity for the most recently run Protocol Executing a new Protocol updates the history log by overwriting previous log To export log to a PC a Hyper Terminal communication protocol must be installed Hyper Terminal communication protocol comes free with Windows Please call for Windows based link Once installed to export information to your PC press F1 PC upload reports to PC Please refer Appendix D Hyper Terminal and USB Driver Setup SYSTEM Data Log 8 21 2006 11 00 Lab Mode 1 Volume 50 ul Protocol completed successfully Run started 06 8 21 13 20 10 Run finished 06 08 21 14 25 00 9 2 Date amp Time Users can change the date type default date and time of this unit by selecting Date amp Time screen as shown below under SYSTEM screen Please remember to press 1 ACCEPT to store your settings 26 MultiGene Thermal Cycler SYSTEM gt DATEG TIME 8 21 2006 11 00 Data TYPE M 1 Y M D 2 M D Y 3 D M Y Default Date 08 21 06 Default Time 11 17 44 Press Fl to save your settings ACCEPT 9 3 Lid Temperature Users can change the temperature of lid of this unit by selecting Lid Temperature screen as shown below under SYSTEM screen Please remember to press F1 ACCEPT to store your settings Two temperature segements 60 65
13. 172 0 4 0 Time 5 00 0 30 10 30 10 00 o Time Increment 0 00 22 MultiGene Thermal Cycler 6 4 3 Pause Setting Position your cursor on desired temperature segment Press F4 PAUSE to add a pause action during operation The Pause will appear on the Temperature Segment graph The pause will last for 10 minutes then the Protocol will resume the operation To remove the Pause setting place the cursor under P Pause symbol Press F4 PAUSE again will remove the Pause setting and its symbol 6 5 Saving a Protocol After all settings are entered press F1 SAVE will store the protocol Screen will Prompt Please enter protocol name A Protocol name is required to be entered Max Protocol name 12 characters 7 Editing Viewing a Protocol An editing Protocol can be saved under a new name newly created Protocol or under the same name edited Protocol Under the User s Folder choose the Protocol to be edited Select F3 EDIT to edit the selected Protocol This displays the existing Protocol and enables editing of the temperature segment and numerical values as shown below Please refer Chapter 6 Creating a New Protocol to edit the temperature segment and numerical values and other advanced functions Press F1 SAVE to save the edited Protocol under the original name or F2 SAVE A to as a new protocol A gt Mike gt EDI 8 21 2006 11 00 Program name A Key0 0 lt gt amp
14. Gently slide the heating block until the plug is exposed then disconnect Gently slide the block until the two screw holes are lined up Problem Cause Action The display is off even when 1 Power is not reaching the Check power source power is switched on system voltage 2 Power cord is not 2 Reconnect the power plugged into the socket cord properly 3 Replace the power fuse 3 Power fuse is blown out 4 Return the unit for 4 Block fuse is blown out service Can t reach 4 C Operating temperature l Operate the unit in a environment environment temperature unsuitable between 15 to 30 C 2 Electronic cooling 2 Return the unit for element may be damaged service or aged Cycle time is too long 1 Operating environment l Operate the system in a temperature temperature environment unsuitable between 15 to 30 C 2 Electronic cooling 2 Return the unit for element may be damaged service or aged 3 Return the unit for 3 Faulty temp sensor service Lid heater does not work 1 Lid heater is set off 1 Check Lid Temperature 2 Lid sensor problem setting in System Mode 2 Return the unit for service No key entry sound 1 Sound may currently be l Check Beeper setting in set off System Mode 2 Faulty keys or 42 Return the unit for wiring service The display goes off 1 Faulty backlight 1 Check the LCD contrast 30 MultiGene The
15. MultiGene Thermal Cycler TC9600 G TC9600 TC6000 G TC6000 Operation Manual Ver 1 4 Labnet International Inc PCR Disclaimer MultiGene Thermal Cyclers can be used for a variety of molecular biology applications and techniques including the Polymerase Chain Reaction PCR PCR and other molecular biology processes and techniques may be protected by patents Purchase of this product does not convey a license or right to perform patented PCR processes Applied Biosystems holds some of the basic PCR patents that may or may not be currently valid For additional information on PCR patents held by Apllied Biosystems and information about acquiring any required licensing for using PCR techniques please contact Applied Biosystems at 800 327 3002 or 650 638 5800 or their website www appliedbiosystems com Table of Contents 1 SAFETY PRECAUTIONNS 2 ks 1 2 GENERAL DESCRIPTION 2 2 2 Vie PERATURES ga naga nn 2 22 PRODUCT OVERVIEW ann eget iva y 2 3 GETTING STARTED eoo o esos pe opaca o dete Co ea ee pras e po Poo 6 Sl UNPACKING ies ta cei eee da i eec eee dte ce IR eek ee 6 INTTTATZOPERATION si a o e tee dee eer 6 S S HEBATED EIDS eto da na rede ea Le it 6 3 4 LID 5 04
16. TURE 5 2 00 00 000000000 0000000 1 19 6 3 CHANGING TEMPERATURE SEGMENT COUNT TEMP AND CYCLE COUNT CYC 19 6 4 ADVANCED SETTINGS ccccsssssscscccccccecssssscsccscccesssssssesccsccseeusesssesccsccsseuseesssscssccseeeeea 19 6 5 SAVING AIPROTOCO OL rerit eve eee 23 7 EDITING VIEWING A PROTOCOL e c eee eee eee ee eeen eee 23 8 DELETING A 1 24 9 SYSTEM SENUR 25 OC DATA LOG Ee tnn Decet chem acd eof nr AA a Du ci a 26 DATES TIME 2 eed oo eto b td d Ecc M D Le Uefa 26 9 3 ID TEMPERATURE 2 5 nnde ie E ate ite Hee a 27 BEE PER M 28 9 5 ADMINISTRATOR kasti siss ll Sr 28 0 6 SERVICE E eee ibestes cbe ee 28 10 MAINTENANCE AND 5 2 29 004 oed Qr DVD ERE Read 29 10 2 CLEANING THE HEATED 0002 0 0 0000000 nhe etes eene 29 LOS REPLACING AFUSE t ee ee 29 10 4 CHANGING THE HEATER 20 6 02 0 0 00000 29 11 TROUBLESHOOTING ececetucccuius cc conto eoo nena
17. angeable blocks Automatically creates operating history and error logs Heated lid to prevent the formation of condensation Robust and attractive design 2 2 Product Overview The front panel of MultiGene thermal cycler includes a display panel and 22 key keypad which consists of function keys F1 F5 alphanumeric keys directional arrow keys and the ENTER key MultiGene Thermal Cycler CE Key sed to clear any entered information STOP Key sed to stop running methods Alphanumeric Keys Function Keys ENTER Key sed to make selections sed Tor con irming in screen display Arrow Keys sed to move cursor and entered information and move between pages selecting functions Figure 1 Front Panel Overview Name Function Function the function keys F1 to FS to activate the button displayed on F1 to F5 the screen above the key for example F1 for RUN Directional The four Directional Arrow keys can move the cursor into different Arrow Keys directions ENTER key The current selection is activated or accepted when the ENTER key is pressed Alphanumeric alphanumeric keys are usually used as number keys for the entry Keys 0 9 of time temperature password and cycle numbers When entering a user name or protocol name a small screen will pop out to display the letters after pressing the Alphanumeric keys Press the selected key until the desired letter is high
18. e settings Press F2 CLEAR to re enter the settings A capital letter and High temperature value G70 0 will appear on the Temperature Segment graph and Low temperature value will be shown on the Temp value of the section To remove the Gradient Temperature setting place your cursor in the same temperature segment with Gradient Temperature symbol Press F2 GRAD Again The screen will show the calculated temperature gradient and High and Low temperature settings Press F2 GR OFF to clear the High and Low temperature settings Press FS EXIT will delete the Gradient Temperature setting and its symbol The Gradient temperature ranges are shown below Temperature setting range 30 0 to 99 0 C Temperature difference 6 0 to 24 0 20 MultiGene Thermal Cycler Note The temperature values between 2 and 11 rows are calculated values and represent an algorithmic based on the heat distribution though out the block Mi ke gt PROTOCOL gt NEW gt GRAD 8 21 2006 11 00 Row 01 02 03 04 05 06 Temp 84 0 84 6 85 2 85 8 86 4 87 0 Row 07 08 09 10 12 87 5 88 0 88 5 89 0 89 5 90 0 Low Temp 84 0 High Temp 90 0 GRADIENT ON 6 4 2 Incremental Decremental Temperature and Time Settings To increase or decrease the dwell time or temperature after each repeat you must have more than one cycle repeat i e 55 C and a dwell time 40 seconds you could decrement the second step
19. e these changes to the original protocol settings Temp Segment 3 Temp Segments Temp Time Values ea Mike gt PROTOCOL gt NEW 8 21 2006 11 00 1 Temp 3Temp 25 Temp 1 Temp 94 0 55 0 72 0 72 00 Time 5 00 0 30 0 30 0 30 10 00 2 JE rs rs 6 1 Changing Temperature Time Values 1 Press directional arrow keys to move cursor to the temperature or time settings required to be changed 2 Enter your desired values and press ENTER or to move cursor to other values to save the settings 18 MultiGene Thermal Cycler The temperature and time ranges are shown below Temperature range 4 0 to 99 9 Time range minute second 00 01 to 99 59 99 99 6 2 Inserting Deleting Temperature Segment Temperature Segment can be inserted or deleted by selecting F2 INSERT F3 DELETE key The inserted or deleted temperature segment is dependent upon the location on the temperature segment where the cursor is positioned If the cursor is positioned inside the first multi temperature time segment pressing F2 F3 will insert delete the whole Multi Temperature Cycle If the cursor is located in any other Multi Temperature Time segment only the selected segment will be inserted deleted 6 3 Changing Temperature Segment Count Temp and Cycle Count Cyc The Temperature Segment Count Temp can be modified by entering a new value 1 to 9 in the
20. ed except by administerator 14 MultiGene Thermal Cycler 5 Protocol Management After entering the selected User Folder users can press F1 F4 keys to Run New Create Edit View and Delete a selected Protocol as shown below Mi ke gt PROTOCOL 8 21 2006 11 00 PROTOCOL DATE TIME Touchdown 07 25 06 09 10 20 Lab 07 28 06 03 25 30 Please select a protocol and press ENTER IT DELETE 5 1 Running a Protocol 1 Press FI RUN to run the selected Protocol under the User folder screen The screen will show as below Mike gt Lab gt RUN 8 21 2006 11 00 1 Temp 3 Temp 25 2 Temp 1 Temp 94 0 94 0 55 0 72 0 72 00 4 00 Time 5 00 0 30 0 30 0 30 10 00 Mode 1 1 2 Volume Efjui 5 100u1 1 Tube Control 2 Block Control START 15 2 Users are required to select a Temperature Control Mode before running the Protocol Mode 1 Tube Control Enable system operation with simulated temperature control If Mode 1 is selected users are required to input the sample volume 5 100ul In this mode temperature control is based on Block temperature and an algorithm which estimates sample temperature based on the block temperature and the sample volume Recommended for more accurate temperature control Mode 2 Block Control Enable system operation with maximum temperature control based on the actual block temperature Recommended for normal
21. lid to the rear 2 lift up the lid The first method is recommended for common operations and to prevent the skin burn from the heated lid The second method is used to clean up the heating plate of the lid Rotate Lid Knob in a clockwise direction to lower down the heating plate and press down on the cap of tubes tighten until you feel resistance This adjustment will provide better contact between the tube or plates and the block for better performance Rotate the Lid Knob in counter clockwise direction as shown in Figure 4 until the Lid pops out Only when the Lid pops out users can slide the Lid to the rear as shown in Figure 5 To lift up the lid for heating plate cleaning please refer Figure 6 8 Figure 4 Rotate Lid Knob to open the lid Figure 5 Slide the lid backward to access the block Figure 6 Slide the lid to align the lid hinge with notches of top case Figure 7 Lift the lid MultiGene Thermal Cycler Figure 8 Rest the lid in vertical position 3 5 Function Map The User Screen includes 5 function options 1 VIEW folders under selected User folder 2 NEW Create a User 3 EDIT a User 4 DELETE a User 5 SYSTEM Once a user folder is selected and the password is approved users can 1 RUN a Protocol 2 NEW Create a protocol 3 EDIT VIEW a Protocol and 4 DELETE a Protocol The function map can is shown in Figure 6 Various functions including Gradient can be added to the selected c
22. lighted Press the ENTER key to select the letter or wait 5 seconds CLEAR key Press CLEAR key will erase the entry in an input field CE STOP key By pressing STOP the Protocol current running will be terminated Sample Block Display Air Vents Front Lid Knob Key Pad Air Vents Left and Right Sides Figure 2 Front View Name Function Lid The lid with heating pad is designed to protect against evaporation resulting from heating of the reaction tube and reaction plate Lid Knob Rotate Lid Knob in clockwise will lower the heating plate to place pressure on the cap of tubes This forces the tubes to sit firmly in the block for better contact Rotate Lid Knob in counter clockwise direction until the Lid pops up Only after the Lid pops can users slide the Lid to the rear Sample Block Section of the unit in which sample tubes and plates are placed Key Pad Used to enter the various Protocols and settings Control Panel which includes Display and Key Pad Display Displays current status of the various system features and functions Air Vents Front for air output Air Vents Left and Right Sides Air Vents Bottom for air output for air intake MultiGene Thermal Cycler Power Cable Connector Power Switch Product Label Air Vents Rear Figure 3 Rear View Brightness Adjustme
23. nt Potentiometer USB Port iis RS 232 Port Name Function Power Cable Power cable socket and fuse Connector Power Switch Power On Off switch Brightness Control the brightness of LCD panel Please use Philip head screw Adjustment driver to adjust it Potentiometer USB Port For protocol or logs transfer to the computer RS 232 Port For protocol or logs transfer to the computer Air Vents Rear For air output Product Label Indicate the model name serial number power specification and other important information 3 Getting Started 3 1 Unpacking Open the MultiGene Gradient non Gradient thermal cycler package and confirm that all items below are included e MultiGene Gradient non Gradient e Operation manual thermal cycler unit e Electric fuses 7A amp 3 15A e AC power cord e Warranty card e 0 5 0 2 ml Labnet tubes If there are any items missing damaged or incorrect items please contact your distributor or sales representatives immediately Note Please use 7A fuse in power source 100 120 50 60Hz countries while 3 15A fuse is for power source 200 220V 50 60Hz countries 3 2 Initial Operation Place the machine on a steady flat table Please keep the machine at least 30cm from the wall or other machine Connect the power cord to the power socket in the rear of the machine Check the power source is compatible with your machine s fuse rating input Pre
24. nts of the User will require you to enter the 4 digit Personal Identification Number PIN as shown in the screen below After the PIN is entered press or ENTER key to confirm the PIN To run a Protocol please refer Section 5 1 USER FOLDERS gt Mike 8 21 2006 11 00 Please enter PI Enter PIN and press ACCEPT Press ACCEPT if not PIN protected ACCEPT 4 2 Creating a New User Folder Under User Folder screen press F2 NEW can create a new User folder After entering the new User name press F1 ACCEPT to accept the new User folder name The MultiGene will prompt you to enter a 4 digit PIN Pressing F1 ACCEPT will save the new folder name and PIN Max User folder name 12 characters Max User folder no 20 4 3 Editing a User Folder Under the User Folder screen use directional arrow keys to select a User folder Press F3 EDIT to edit the User name A PIN is required to edit the User folder name After editing the User folder name press F1 ACCEPT to save the edited User name 13 4 4 Deleting a User Folder Use directional arrow keys to select a User folder Press F4 DELETE to delete the selected User folder You are required to enter the PIN and press F1I ACCEPT or ENTER The screen will prompt Do you want to delete Press FI ACCEPT to confirm the deletion Press F5 EXIT to abort the deletion Note User folders which contain any protocols can t be delet
25. ommunication tab and click Click Hyper Terminal You will be prompted by default telnet program select NO Name the connection link Select the com port COM3 Port settings a Bits sec 9600 b Parity None c Stop bits 1 b Flow control None USB Driver Installation 1 DOS IND Place CD into computer select PL 2303 Driver on disc Follow install process once program is installed click finished tab Connect the MiltiGene to your computer by the USB cable Click the start tab and go to Control Panel and double click Go to System icon and double click In System select the Hardware tab In Hardware tab select Device manager Under ports select the Serial comport COM 3 36 MultiGene Thermal Cycler Appendix E MultiGene Recommended Consumables Consumables Pack Description Catalog Size Number Plates TC96 NS 10 10 96 Well Polypropylene Clear No Skirt TC96 NS 10 10 96Well Polypropylene Clear Elevated skirt 8 02 20 0 2 ml 1 x 8 Tube Clear TCF8 02 A 20 0 2 ml 1 x 8 Tube assorted colors TCD8 02 C 20 0 2ml 1x8 domed Tube s clear TCD8 02 A 20 0 2 ml 1 x 8 domed Tube assorted colors Consumables Pack Description Catalog Size Number Sealing Mats Tape TC 96 MAT 20 Sealing Mat 96 well Microplate TC96 AS 100 100 Thermal Tape PCR AS 200 100 Aluminum Sealing Tape Consumables Pack Description Catalog Size Number Compression Mat TC96 CM 10 10 Silicone Compression Ma
26. pressure on the plates Note To avoid deformation of the sample tubes or Strips it s important to load at least one tube in each of the four corners and at least four tubes in the center of the block this will distribute the pressure evenly throughout the surface 11 4 User Folder Management After the unit is turned on the initial screen which contains software version and manufacturer s name will show up for 10 seconds and then enter User Folder screen Pressing any key will go to User Folder screen immediately A sample screen is shown below Directory User Folder Name Status Bar Date amp Time USER FOLDER 8 21 2006 11 00 PUBLIC Lab Mike Susan Jeff Select a user folder OPEN NEW DI DELETE SYSTEM Users can use the function keys F1 F4 above to view create edit and delete the User folders FS SYSTEM is used for system settings Note 1 The PRESET and PUBLIC folders are pre registered folders which require no Personal Identification Number PIN to enter 2 The PRESET folder includes some frequently used Protocols defined by vendor while the PUBLIC folder is open to all users 4 1 Viewing a User Folder All Protocols are stored inside the User folders Use directional arrow keys to select a User folder Press 1 to view the names of all Protocols inside the selected folder 12 MultiGene Thermal Cycler To view the conte
27. reated Protocol User Folder OPEN NEW EDIT DELETE SYSTEM User User User User RUN NEW EDIT DELETE VIEW Protocol Protocol Protocol Lid Temp Administrator Insert Delete Advanced Gradient Increment Pause Save Figure 9 Operation Flowchart 10 MultiGene Thermal Cycler 3 6 Key Entry System Note that the alphanumeric keys are assigned multiple characters selectable by repeatedly pressing the key to cycle through the available characters The highlighted character or number will automatically be entered into the name of a newly created or edited Protocol or User after a very short period of time if users don t continue to press the key Key No Key Representation 0 space lt gt amp 90 C a b 2 D E F d f 3 G H I g h i 4 J K L j k 1 5 M N O m n 0 6 P Q R S 9 5 7 T U V t u v 8 RAJUIN W X Y Z W Z 9 3 7 Loading the Reaction Vessel For the optimal performance of the MultiGene we recommend Labnet tubes and plates The recommended sample volume for 0 2 ml tubes or 96 well plates is 5 100ul while the 0 5 ml tubes is 50 200ul If using the heated lid with 96 well plates it is highly recommended to use a Silicone Compression Mat Labnet TC96 CM 10 Axygen 521 01 601 to assist in placing even
28. rmal Cycler 2 Faulty LCD panel 2 Return the unit for service Display is too dark or light Backlight brightness is not Adjust Display Brightness adjusted properly Potentiometer Lid will not close 1 Foreign matter between Remove the foreign object or lid heater and sample matter block 2 Faulty lid lock mechanism Error message appears Refer to list of error Check the nature of the error messages below and take the suggested actions 11 1 Error Messages Message Cause Action HEATER ERROR Lid temperature sensor problem Reboot the unit e Lid temperature exceeds 132 C e Lid temperature can t reach the set temperature BLOCK ERROR Block temperature sensor or heating Cool down the room element problems temperature Reboot the unit e The unit can t reach the set temperature because the room temperature is too high e The block temperature exceeds 108 C ROOM ERROR Room temperature sensor problem Reboot the unit ROOM gt 30 C e The room temperature exceeds 30 Cool down the room e Two running units are too close to each other temperature Reboot the unit Remove any objects blocking the vents Keep two running units at least 30cm from each other 31 Appendix A Technical specifications Model Name TC9600 G TC9600 TC6000 G TC6000 Sample Capacity 0 2ml x 96 tubes 0 5ml x 60 tubes Temperature Ranges 4 99 9 4 99
29. source conforms to the required power supply specifications To avoid electric shock make sure the machine is plugged into a grounded electrical outlet Do not allow water or any foreign objects to enter the various openings of the machine Switch off the machine before cleaning or performing service on the machine such as replacing the fuses Repairs should be carried out by authorized service personnel only HOT N High Temperature Label Please be aware of the heated block AN Warning Label Please be aware of the danger of electric shock or other dangers 2 General Description The MultiGene Gradient thermal cycler is a powerful reliable and affordable thermal cycler It is equipped with a gradient function that enables the user to set a maximum 24 C temperature gradient between the left and right rows in the sample block at each temperature 2 1 Features Easy to operate slide and lever type lid which prevents accidental skin burn Large LCD panel enhances visibility and ease of operation Graphic display makes programming quick and simple Simple and easy to use graphical interface Extended Temperature return function that makes touchdown possible Gradient function enables optimization of annealing temperatures Preset protocols that make starting amplification easier Air intake vents on the front and sides and exhaust vents on the rear that reduce heat based interference with other equipment Interch
30. ss Power On Off Button to switch on the machine The screen will show the software version for 10 seconds and then enter User Folder screen Pressing any key will go to User Folder screen immediately 3 3 Heated Lid The Heated Lid heats the air in the upper part of the sample vessels at higher temperature than the reaction mixture This prevents condensation of the evaporated water vapor on the vessel walls keeping the concentration unchanged during the heating and cooling cycles The top also places pressure on the caps of tubes and film on titer plates to prevent vapor loss and cross contamination of samples When using plates it is highly recommended using a Silicone Compression Mat Labnet TC96 CM 10 to assist in placing even pressure on the plates Note 1 If MultiGene is started cold approximately two to three minutes will be required 6 MultiGene Thermal Cycler before heated lid is at operating temperature 2 To avoid heat dissipation the Heated Lid should be closed and tighten during a protocol run Keeping the heated lid open during a protocol run might cause HEATER ERROR because the heated lid fails to reach the set temperature 3 To evaporate the condensation water inside sample block and to avoid HEATER ERROR users should turn off the Lid Temperature under SYSTEM mode before heating the sample block and keeping the lid open 3 4 Lid Opening Closing There are two ways to open the lid 1 Slide the
31. t Sealing Tape Clear sealing tape will seal 96 and 384 well Plates when used with Labnets compression mat this polypropylene film eliminates well to well contamination and cross over in PCR applications Functional Temperature range 40 C to 104 C Cat TC96 TT Sealing Tape Aluminum sealing tape will seal 96 and 384 well Plates when used with Labnet s 37 compression mat this polypropylene film eliminates well to well contamination and cross over in amplification applications Ideal for light sensitive samples and its uniformly applied adhesive provides optimum well sealing Functional Temperature range 80 C to 104 C Cat TC AS 100 Silicone Compression Mat is used to seal 96 well plates when using the MultiGene Thermal Cycler heated lid function These silicone mats can be cleaned and sterilized by either 10 hypochlorite solution and ddi water rinse or by autoclaving at 121 C for 15 minutes Cat TC96 CM 100 38
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