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Agilent 1260 Infinity High Performance Degasser User Manual

1. gt Do not immerse tube end in waste liquid gt Do not intubate tubes in other tubes gt For correct tubing follow instructions on label attached to the module 26 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 Installation Information on Leak and V Warning Attention Warnung Attenzione Advertencia Disconnect power cord before moving D branchez le cordon secteur avant tout d placement gt Entfernen Sie das Netzkabel bevor Sie das Gerat bewegen g Scollegare il cavo di alimentazione prima di spostare lo strumento Desconectar el aparato de la red antes de moverlo b 2J l Figure 6 Warning label illustration for correct waste tubing 1260 Infinity High Performance Degasser User Manual 27 3 Installing the Degasser Installing the Vacuum Degasser Parts required Hardware required Preparations Description Power cord Remote Cable see Cable Overview on page 70 Degasser Locate bench space Provide power connections Unpack the vacuum degasser module WARNING Abnormal conditions In case of abnormal conditions during operation the instrument must be disconnected from line gt To disconnect the instrument from line unplug the power cord CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good co
2. aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides 1260 Infinity High Performance Degasser User Manual 45 4 46 Using the Degasser Stainless Steel ST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be corroded by acids below pH 2 3 It can also corrode in following solvents Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens e High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCly O2 gt 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying pr
3. ot Agilent Technologies Notices Agilent Technologies Inc 2011 2013 2014 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64225 90002 Edition 02 2014 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separa
4. e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable 1260 Infinity High Performance Degasser User Manual 89 10 Hardware Information 90 The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table5 RS 232C Con
5. for any other equipment 1260 Infinity High Performance Degasser User Manual 15 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 17 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 16 1260 Infinity High Performance Degasser User Manual Physical Specifications Site Requirements and Specifications 2 Table 1 Physical Specifications Type Specification Comments Weight 5 kg 11 Ibs Dimensions 80 x 345 x 435 mm height x width x depth 3 1 x 13 5 x 17 inches Line voltage 100 240 V 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 30 VA 30 W 102 BTU Maximum Ambient operating t
6. BNC Pin Agilent Signal Name module O Shield Shield Analog ete Ne Center Center Analog 72 1260 Infinity High Performance Degasser User Manual Agilent Module to General Purpose Identifying Cables 9 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog S e 1260 Infinity High Performance Degasser User Manual 73 9 Identifying Cables Remote Cables 74 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Infinity High Performance Degasser User Manual Identifying Cables 9 Agilent Module to 3396 Series III 3395B Integrat
7. Description Vacuum Degasser Power cord G4800 64005 LC HW User Information Utilities DVD G1379 68705 Accessory Kit for 1260 Infinity High Performance Degasser 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 Optimizing the Stack Configuration If your module is part of a complete Agilent Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume 1260 Infinity High Performance Degasser User Manual 21 3 Installing the Degasser Optimizing the Stack Configuration One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration See Figure 3 on page 22 and Figure 4 on page 23 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required Solvent cabinet Vacuum degasser Instant Pilot Autosampler Column compartment Detector Figure 3 Recommended Stack Configuration for 1260 Infinity Front View 22 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 Optimizing the Stack Configuration Remote cable CAN Bus cable to Instant Pilot AC power CAN Bus cable Analog detector signal 1 or 2 outputs _SSSS55 per detector LAN to LC ChemStati
8. Fuse 250V T 500 mA compatible to all supported line voltages The module power supply has wide ranging capability see Table 1 on page 17 It accepts any line voltage in the range mentioned in the table Consequently there is no voltage selector in the rear of the module There are two externally accessible fuses that protect the power supply These fuses are identical for all accepted line voltages Potential hazard of physical injury or death or damage to the instrument Using fuses other than the one specified for this module and purpose can lead to personal injury or damage of the electronic components gt Never use fuses other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1260 Infinity High Performance Degasser User Manual 63 7 64 Maintenance Switch off the power switch at the front of the instrument Remove the power cable from the power connector at the rear of the instrument Press down the clip of the fuse holder and pull out of the power socket Fuse holder 4 Remove the fuses from the fuse holder 5 Ensure the
9. filter is partly blocked no solvent or only very little solvent will drip out of the solvent tube Cleaning the Solvent Filters Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 85 for one hour Thoroughly flush the filter with bidistilled water remove all nitric acid e Replace the filter Never use the system without solvent filter installed 1260 Infinity High Performance Degasser User Manual Using the Degasser 4 Solvent Information Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see pump manuals e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Material Information Materials in Flow Path Following materials are used in the flow path of this module Table3 Materials in flow path Part Materials Internal tubings TFE PDD copolymer PFA Inlets PEEK Tubings FEP Fittings ETFE 1260 Infinity High Performance Degasser User Manual 43 4 44 Using the Degasser Material Information Materials in the flow path are carefully selected based on Agilent s
10. in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Infinity High Performance Degasser User Manual In This Guide In This Guide This manual covers the Agilent 1260 Infinity High Performance Degasser G4225A 1 Introduction This chapter gives an introduction to the module instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Degasser This chapter gives information about the preferred stack setup for your system and the installation of your module 4 Using the Degasser This chapter explains how to use the module 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces 7 Maintenance This chapter describes the maintenance of the module 1260 I
11. information about the Agilent 1260 Infinity High Performance Degasser G4225A The manual describes the following introduction e site requirements and specifications e installing the degasser using the degasser e troubleshooting and diagnostics e maintenance parts for maintenance e identifying cables e appendix Agilent Technologies 2011 2013 2014 Printed in Germany 02 2014 64225 90002 fe Agilent Technologies
12. operating temperature 17 number of channels 18 0 operating Altitude 17 operating temperature 1 7 optimization stack configuration 21 P packaging damaged 20 parts accessory kit 68 cover 66 damaged 20 missing 20 performance specifications 18 pHrange 18 physical specifications 17 power cable 55 power consideration 14 power consumption 17 power cords 15 power supply 63 power switch 29 pressure sensor 8 priming witha pump 35 with a syringe 35 principle of operation 8 radio interference 98 remote cable 55 remote 102 cable 74 RS 232C cable 81 S safety class safety W waste electrical and electronic equipment 97 WEEE directive 97 94 weight 17 general information 94 standards symbols lid 96 site requirements power cords 15 solenoid valve solvent filters checking cleaning prevent blo sound emission 63 42 42 cking 42 99 special interfaces 92 specification performance specifications 18 physical status indicator syringe adapter 17 04 56 56 56 36 system setup and installation optimizing stack configuration 21 T transportation troubleshooting 37 status indicators 53 V vacuum pump voltage range 8 17 voltage selector 63 volume per cha nnel 18 1260 Infinity High Performance Degasser User Manual Index 1260 Infinity High Performance Degasser User Manual 103 www agilent com In This Book This manual contains
13. solvent The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 5 L For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets a printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet The leak pan 2 individually designed in each module guides solvents to the front of the module The concept covers also leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached The leak pan s outlet port 8 A guides excessive overfill from one module to the next as the solvent flows into the next module s leak funnel 8 B and the connected corrugated waste tube 3 C The corrugated waste tube guides the solvent to the next lower positioned module s leak tray and sensor The waste tube of the sampler s needle wash port 4 guides solvents to waste The condense drain outlet of the autosampler cooler 5 guides condensate to waste The waste tube of the purge valve 6 guides solvents to waste The waste tube connected to the leak pan outlet on each of the bottom instruments 7 guides the solvent to a suitable waste container 1260 Infinity High Performance Degasser User Manual 11 1 introduction System Overview 12 1260 Infinity High Performance Degasser User Manual 1260 Infinity High
14. the main cover can be performed by the user 60 1260 Infinity High Performance Degasser User Manual Maintenance 7 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 1260 Infinity High Performance Degasser User Manual 61 7 Maintenance Assembling the Main Cover When If cover is broken Tools required Description None Parts required p n Description 1 5065 9989 Cover kit includes base top left and right CAUTION Wrong assembly Once installed wrongly you may not be able to remove the side from the top part gt Make sure to install the side parts in the correct orientation 1 Place the top part on the bench and insert the left and 2 Replace the cover right side into the top part Next Steps 3 Replace the vacuum degasser in the stack and reconnect the cables and capillaries 4 Turn on the vacuum degasser 62 1260 Infinity High Performance Degasser User Manual Maintenance 7 Exchanging the Fuses of the Power Inline Filter When Tools required Parts required When defective Description None p n Description 2 2110 0458
15. 3396 integrators Agilent module to general purpose 1260 Infinity High Performance Degasser User Manual CAN cables p n 5181 1516 5181 1519 LAN cables p n 5023 0203 5023 0202 Identifying Cables 9 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n G1103 61611 RS 232 cables p n G1530 60600 RS232 61601 5181 1561 Description General Purpose Cable Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m 1260 Infinity High Performance Degasser User Manual 71 9 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 7 oe 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin
16. 5 1260 Infinity High Performance Degasser User Manual Contents 7 Maintenance 57 Warnings and Cautions 58 Introduction to Maintenance 60 Cleaning the Module 61 Assembling the Main Cover 62 Exchanging the Fuses of the Power Inline Filter 63 Parts for Maintenance 65 Cover Parts 66 Fuse 67 Accessory Kit 68 Identifying Cables 69 Cable Overview 70 Analog Cables 72 Remote Cables 74 BCD Cables 77 CAN Cable 79 External Contact Cable 80 Agilent Module to PC 81 Agilent 1200 Module to Printer 82 10 Hardware Information 83 Operating Principle of the 1260 Infinity High Performance Degasser 84 Electrical Connections 85 Interfaces 86 11 Appendix 93 General Safety Information 94 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 97 Radio Interference 98 Sound Emission 99 Agilent Technologies on Internet 100 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual e Introduction Introduction to the Vacuum Degasser 8 System Overview 9 Leak and Waste Handling 9 This chapter gives an introduction to the module instrument overview and internal connectors if Agilent Technologies 1 introduction Introduction to the Vacuum Degasser The Agilent 1260 Infinity High Performance Degasser model G4225A comprises four separate vacuum chambers with semipermeable tubings a vacuum pump and control assembly When the vacuum d
17. B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes G4204A Quat Pump G1361A Prep Pump 2 Yes No Samplers G1329B ALS 2 Yes No G2260A Prep ALS G1364B FC PS 2 Yes No G1364C FC AS G1364D FC uS G1367E HiP ALS K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes Yes Yes Yes Yes Yes No No No No No Yes Yes Yes Yes Yes Yes CAN DC OUT for CAN slaves CAN DC OUT for CAN slaves THERMOSTAT for G1330B K1330B THERMOSTAT for G1330B K1330B CAN DC OUT for CAN slaves 86 1260 Infinity High Performance Degasser User Manual Hardware Information 10 Table4 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A
18. C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed 1260 Infinity High Performance Degasser User Manual 87 10 Hardware Information Table4 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 88 1260 Infinity High Performance Degasser User Manual Hardware Information 10 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card
19. Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING aia alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 96 1260 Infinity High Performance Degasser User Manual Appendix 11 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To ret
20. Performance Degasser User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 14 Physical Specifications 17 Performance Specifications 18 This chapter provides information on environmental requirements physical and performance specifications ott Agilent Technologies 13 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 17 Consequently there is no voltage selector in the rear of the module Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connecto
21. a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 94 1260 Infinity High Performance Degasser User Manual Appendix 11 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried ou
22. a pump see Priming the Degasser with the Pump on page 35 fails 36 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 Transporting the Vacuum Degasser WARNING Solvents leaking out Solvents remaining in the solvent channels may leak out during transport This can possibly cause personal damage gt Drain any remaining solvents from the degassing channels before transporting the degasser Pull the solvent inlet tubing out of the solvent bottle of channel A 2 Let the pump draw solvent and air through channel A of the degasser until the chamber of channel A is completely dry 3 Repeat these steps for the remaining solvent channels 1260 Infinity High Performance Degasser User Manual 37 3 Installing the Degasser Transporting the Vacuum Degasser 38 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual 00 ee 4 Oad e 07o Using the Degasser i Leak and Waste Handling 40 When to Use a Vacuum Degasser 41 Prevent Blocking of Solvent Filters 42 Solvent Information 43 Material Information 43 This chapter explains how to use the module Apg Agilent Technologies 39 4 Using the Degasser Leak and Waste Handling WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with thes
23. ains salt 34 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 Priming the Degasser with the Pump The vacuum degasser can be primed by drawing solvent through the degasser by pumping with the connected pump For priming the vacuum degasser using the pump the following is recommended 1 Connect all tubings to and from degasser and pump 2 Open the pump s purge valve to waste 3 Pump at a flow rate of about 5 ml min until the solvent reaches the pump Pumping at higher flow rates will stress the system and might result in a failure to prime the degasser with the pump 4 Prime each channel of the degasser and pump individually with at least 5 mL of solvent When the pump has been turned off for a certain time for example overnight oxygen will rediffuse into the solvent channels between the degasser and the pump Volatile fractions of a solvent mixture can evaporate from the solvent if left in the degasser with no flow for an extended time causing a composition change of the mixture Therefore priming of the vacuum degasser and the pumping system is required before starting an application In case priming with the pump should fail the degasser can be primed with a syringe This might happen because the used solvent is highly volatile or the degasser and pump inlet lines are completely dry and the pump therefore fails to draw the solvent from the solvent bottle through the degasser channels into the pu
24. amp is green and are recognized by the connected user interface module parameters can be set module specific screens appear and so on then do the following to determine the problem with the vacuum degasser V Ensure the power cable is connected to the degasser and the power cable is connected to line power V Ensure the power switch on the front of the module is ON V Ensure the power fuses are OK The fuse holders are located on the rear panel of the vacuum degasser and are part of the power socket Check the fuses see Exchanging the Fuses of the Power Inline Filter on page 63 and change if necessary V If the previous steps did not solve the problem contact your Agilent service representative 1260 Infinity High Performance Degasser User Manual 55 6 56 Troubleshooting and Diagnostics If the Status Indicator is Red Sufficient vacuum is normally built up after the initial start up and controlled by the pressure sensor If the vacuum cannot be reached the vacuum degasser will switch to the error state The error condition can be reset by turning the vacuum degasser off and on again If the Status Indicator is Yellow and the Vacuum Pump is not The status indicator is yellow during the startup phase of the degasser until the operating pressure is reached This may take several minutes Please contact your Agilent service representative Status Indicator becomes Red and Vacuum Pump was Running Suffic
25. ble in a wide pH range and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc 1260 Infinity High Performance Degasser User Manual Using the Degasser 4 Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic
26. cal shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 58 1260 Infinity High Performance Degasser User Manual Maintenance 7 WARNING Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 1260 Infinity High Performance Degasser User Manual 59 7 Maintenance Introduction to Maintenance The vacuum degasser is designed for easy repair The most frequent repairs such as exchanging power fuses and assembling
27. dissolved oxygen especially in reduction mode 1260 Infinity High Performance Degasser User Manual 41 4 Using the Degasser Prevent Blocking of Solvent Filters 42 Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the pump This is especially true for aqueous solvents or phosphate buffers 4 7 pH The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the pump e Use sterile if possible amber solvent bottles to slow down algae growth Filter solvents through filters or membranes that remove algae e Exchange solvents every two days or refilter e If the application permits add 0 0001 0 001 M sodium azide to the solvent Place a layer of argon on top of your solvent e Avoid exposure of the solvent bottles to direct sunlight Checking the Solvent Filters The solvent filters are on the low pressure side of the pumping system A blocked filter therefore does not affect the pressure readings of the pump The pressure readings cannot be used to identify blocked filters If the solvent cabinet is placed on top of the vacuum degasser the filter condition can be checked in the following way Remove the tubing at the inlet port of the vacuum degasser If the filter is in good condition the solvent will freely drip out of the solvent tube due to hydrostatic pressure If the solvent
28. e a Vacuum Degasser Unspecified Conditions Operating the instrumentation under conditions other than its intended use might result in a potential safety hazard or might damage the instrumentation gt Never operate your instrumentation under conditions other than those specified by the vendor For flow rates less than 10 mL min if your detector is used with maximum sensitivity in the low UV wavelength range if your application requires optimum injection precision if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 mL min if your sample or detection is sensitive to dissolved oxygen in the mobile phase degradation with a fluorescence detector with an LC MS detector For capillary LC application with our Agilent 1260 Infinity Capillary and Nano LC System Generally a degasser should be used when negative effects due to dissolved gas in the mobile phase exceed the limits that are acceptable for the user Negative effects that can be caused by dissolved gas are Unstable flow due to unstable pumping conditions This may result in an increased pressure ripple or increased standard deviations of peak retention times and peak areas especially at low flow rates Baseline noise on detectors that are sensitive to changes in the refractive index sample degradation fluorescence quenching due to dissolved oxygen baseline drift in electrochemical detectors due to
29. e substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet gt The residual free volume in the appropriate waste container must be large enough to collect the waste liquid gt Check the filling level of the waste container regularly gt To achieve maximal safety check the correct installation regularly 40 Recommendations for Solvent Cabinet For details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets For details on correct installation see Installation Information on Leak and Waste Handling on page 24 1260 Infinity High Performance Degasser User Manual Using the Degasser 4 When to Us
30. egasser is switched on the control assembly turns on the vacuum pump which generates a low pressure in the vacuum chambers The pressure is measured by a pressure sensor The vacuum degasser maintains the low pressure by a controlled leak in the air inlet filter and a regulation of the vacuum pump using the pressure sensor The LC pump draws the solvents from their bottles through the semipermeable tubes of the vacuum chambers When solvents pass through the vacuum chambers any dissolved gas in the solvents permeates through the tubings into the vacuum chambers The solvents will be degassed when leaving the outlets of the vacuum degasser Control Sensor or circuit 4 separate vacuum chambers Solvent Vacuum container Figure Overview only one of the four solvent channels is shown 8 1260 Infinity High Performance Degasser User Manual Introduction 1 System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed 1260 Infinity High Performance Degasser User Manual 9 1 Introduction System Overview Figure 2 Leak and waste handling concept overview typical stack configuration as an example 10 1260 Infinity High Performance Degasser User Manual Introduction 1 The solvent cabinet 1 is designed to store a maximum volume of 6 L
31. el is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 82 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual 10 Hardware Information Operating Principle of the 1260 Infinity High Performance Degasser 84 Electrical Connections 85 Rear view of the module 85 Interfaces 86 Overview Interfaces 89 This chapter describes the degasser in more detail on hardware and electronics ott Agilent Technologies 83 10 Hardware Information Operating Principle of the 1260 Infinity High Performance Degasser 84 During initialization the status indicator is amber and the degasser vacuum pump tries to reach a target pressure below 146 mbar hPa If this limit cannot be reached within 10 minutes an error is thrown and the status indicator gets red As soon as the limit has been reached which typically happens after about 2 minutes the pump goes to a continuous regulation mode where the pump tries to regulate to 100 mbar hPa target pressure If this pressure limit cannot be reached it is increased stepwise to a maximum of 146 mbar and the status indicator goes to amber If a stable pressure between 100 mbar and 146 mbar max can be kept the status indicator switches to off If the maximum limit of 146 mbar is exceeded the status indicator goes to red and an error is thrown 1260 Infinity High Performance Degass
32. emperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 0 55 C 32 131 F 40 70 C 40 158 F lt 95 r h at 40 C 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation category II Pollution degree 2 Non condensing For storing the module For indoor use only 1 This temperature range represents the technical specifications for this instrument The tempera tures mentioned may not be suitable for all applications and all types of solvent 1260 Infinity High Performance Degasser User Manual 17 2 Site Requirements and Specifications Performance Specifications 18 Table2 Performance Specifications Agilent 1260 Infinity High Performance Degasser Type Specification Number of solvent channels Flow range Internal volume per channel Materials in contact with solvent pH range 4 0 10 mL min per channel 0 45 mL per channel TFE PDD Copolymer FEP PEEK 1 14 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual 3 Installing the Degasser Unpacking the Vacuum Degasser 20 Optimizing the Stack Configuration 21 One Stack Configuration 22 Installation Information on Leak and Waste Handling 24 Installing the Vacuum Degasser 28 Flow Connections to the Vacuum Degasser 31 Operational Hints for the Vacuum Dega
33. er User Manual Hardware Information 10 Electrical Connections e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Rear view of the module Figure 15 Rear View of Degasser Electrical Connections and Label 1260 Infinity High Performance Degasser User Manual 85 10 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table4 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN optional on board RS 232 Analog APG Remote Special Pumps G1310B Iso Pump 2 Yes No G1311
34. experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is sta
35. fuse wires inside the fuses are not broken If a test meter is available check the resistance of each fuse A good fuse shows a low resistance typically less than 1 Ohm If a fuse is defective wire broken or high resistance insert a new fuse 7 Reinsert the fuse holder and the power cable Switch on the power switch 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual m Parts for Maintenance o J g Cover Parts 66 4 Fuse 67 Accessory Kit 68 This chapter provides information on parts for maintenance ee Agilent Technologies 65 8 Parts for Maintenance Cover Parts Cover Parts Item p n 1 5065 9989 2 5043 0207 3 5065 9990 4 5041 8365 5 5041 8387 Figure 13 Cover Parts Description Cover kit includes base top left and right Name plate 1260 Front cover Blank plug for unused channels Tube clip 1260 Infinity High Performance Degasser User Manual Parts for Maintenance 8 Fuse Fuse Item p n Description 1 2110 0458 Fuse 250V T 500 mA compatible to all supported line voltages Figure 14 Fuse 1260 Infinity High Performance Degasser User Manual 67 Parts for Maintenance Accessory Kit 68 Item p n G1379 68705 G1379 68706 G1322 67300 5062 2461 0100 1710 5061 3378 Description Accessory Kit for 1260 Infinity High Performance Degasser Connecting tubing to connect to chan
36. g the Degasser 3 Flow Connections to the Vacuum Degasser A Bottle head assembly Solvent cabinet Pressure sensor Static mixer Purge valve Pump head channel A Pump head channel B Active inlet valve B Active inlet valve Waste tubing Outlet capillary to autosampler Waste outlet Figure 10 Flow Connection to the Vacuum Degasser 1260 Infinity High Performance Degasser User Manual 33 3 Installing the Degasser Operational Hints for the Vacuum Degasser General Priming Instructions WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Before using a new degasser or new tubings for the first time 1 Prime all tubings with at least 5 ml of iso propanol no matter whether the channels will be used with organic mobile phase or with water If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows 2 Replace the current solvent with iso propanol if current solvent is organic or with water if current solvent is an inorganic buffer or cont
37. hooting and Diagnostics Overview of the Degasser s Indicators 52 Status Indicators 53 Module Status Indicator 54 Hardware Symptoms 55 All Lamps are Off 55 If the Status Indicator is Red 56 If the Status Indicator is Yellow and the Vacuum Pump is not Running 56 Status Indicator becomes Red and Vacuum Pump was Running 56 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces ott Agilent Technologies 51 Troubleshooting and Diagnostics Overview of the Degasser s Indicators 52 Status Indicators The vacuum degasser is provided with two status indicators which indicate the operational state ready busy and error states of the vacuum degasser The status indicators provide a quick visual check of the operation of the vacuum degasser see Figure 12 on page 53 Hardware Symptoms A red status lamp at the vacuum degasser indicates a problem with the vacuum system or with the electronic control The vacuum degasser generates an error output on the remote lines The following pages describe hardware symptoms which help you to isolate the cause of a hardware failure see Hardware Symptoms on page 55 1260 Infinity High Performance Degasser User Manual Troubleshooting and Diagnostics 6 Status Indicators Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicate
38. ides and concentrated acids like aqua regia Zirconium Oxide ZrO Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons TFE PDD copolymer tubings which are used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To ensure the longest possible life with HFIP it is best to dedicate a particular chamber to this solvent not to switch solvents and not to let dry out the chamber For optimizing the life of the pressure sensor do not leave HFIP in the chamber when the unit is off Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide Al Og are inert to almost all common acids bases and solvents There are no documented incompatibilities f
39. ient vacuum is normally built up after the initial start up and controlled by the pressure sensor If the vacuum cannot be reached the vacuum degasser will switch to the error state The error condition can be reset by turning the vacuum degasser off and on again The following parts can be responsible for an insufficient vacuum 1 Leaky tubing 2 leaky or defective internal parts Contact your Agilent service representative 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual 7 Maintenance Warnings and Cautions 58 Introduction to Maintenance 60 Cleaning the Module 61 Assembling the Main Cover 62 Exchanging the Fuses of the Power Inline Filter 63 This chapter describes the maintenance of the module ee Agilent Technologies 57 7 Maintenance Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electri
40. is followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 1260 Infinity High Performance Degasser User Manual 91 10 Hardware Information Table6 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or fi
41. main firmware A fast blinking indicator indicates that the module is in boot loader mode e g during update of main firmware In such a case try to re boot the module or try a cold start CAUTION Red status LED indicates error This indicates either an internal leak in the vacuum system or an electronic failure gt To prevent any damage switch off the vacuum degasser and remove the solvent bottles from the solvent cabinet to stop any gravity caused flow of solvent into the vacuum chamber gt Incase of an internal leak it is possible that solvent may enter the vacuum chamber and solvent may leak into the waste drain gt Apply troubleshhoting information in If the Status Indicator is Red on page 56 54 1260 Infinity High Performance Degasser User Manual Troubleshooting and Diagnostics 6 Hardware Symptoms In case of a problem with the vacuum system or the electronic control the vacuum degasser status lamp will be red The vacuum degasser will generate an error output on the remote lines This will shut down other system modules when connected via remote cable see Installing the Vacuum Degasser on page 28 The vacuum degasser itself is not able to generate any error messages in the Agilent 1260 Infinity system logbook The following pages describe hardware symptoms which help you to isolate the cause of a hardware failure All Lamps are Off If all other modules in the system are on power switch l
42. mp 1260 Infinity High Performance Degasser User Manual 35 3 Installing the Degasser Priming the Degasser with a Syringe only recommended if priming with the pump fails CAUTION Damage to degasser Drawing the solvent through the degasser very quickly might destroy the chambers gt Draw the solvent through the degasser channels with moderate speed in order to avoid damage to the chambers 1 Disconnect solvent outlet tube of the channel that is supposed to be primed from your pump 2 Connect syringe adapter to solvent outlet tube Push syringe adapter onto syringe 4 Pull syringe plunger to draw at least 5 mL of solvent through degasser and tubing 5 Replace the priming solvent with the new solvent of your choice 6 Pull syringe plunger to draw at least 5 mL of solvent through degasser and tubing 7 Disconnect syringe adapter from solvent tube 8 Connect solvent tube to your pump 9 Repeat step 1 on page 36 to step 8 on page 36 for the other solvent channels When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes faster compared to priming with a pump The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 5 minutes with your selected flow rate before starting any application This will allow the vacuum degasser to properly degas the solvent in the degasser tubes Priming the degasser with a syringe is only recommended if priming with
43. ndition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 28 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 1 Place the vacuum degasser on the bench 2 Ensure the power switch on the front of the vacuum degasser is OFF switch stands out 3 Connect the power cable to the power connector at the rear of the vacuum degasser 4 Connect the interface cable to the vacuum degasser The remote cable is a one way connection to send a not ready signal from the degasser to the other modules or to shut down down the whole system after an error condition of the degasser In an Agilent 1260 Infinity stack the individual modules are connected through a CAN cable The Agilent 1260 Infinity High Performance Degasser is an exception The degasser can optionally be connected via the remote cable to the other modules of the stack An Agilent 1260 Infinity Instant Pilot can be connected to the CAN bus at any of the modules in the system except for the degasser The control software can be connected to the system through a LAN cable via LAN Card to the detector or any other module except the degasser if no detector is present Power socket Remote cable socket RS 232 socket Fuse holder Figure 7 Rear of the Vacuum Degasser 1260 Infini
44. nection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 2 2 RX TX 3 XxX 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 A 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 16 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1260 Infinity High Performance Degasser User Manual Hardware Information 10 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analys
45. nels in series for increased performance 2x Kit of 4 solvent tubes including labels for connection degasser to MCGV Quaternary Pump Waste tube 5 m reorder pack Mounting Tool for Tubing Connections Remote Cable 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual 9 Identifying Cables Cable Overview 70 Analog Cables 72 Remote Cables 74 BCD Cables 77 CAN Cable 79 External Contact Cable 80 Agilent Module to PC 81 Agilent 1200 Module to Printer 82 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ott Agilent Technologies 69 9 Identifying Cables Cable Overview 70 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 74 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to
46. nfinity High Performance Degasser User Manual 3 In This Guide 8 Parts for Maintenance This chapter provides information on parts for maintenance 9 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 10 Hardware Information This chapter describes the degasser in more detail on hardware and electronics 11 Appendix This chapter provides addition information on safety legal and web 4 1260 Infinity High Performance Degasser User Manual Contents Contents 1 Introduction 7 Introduction to the Vacuum Degasser 8 System Overview 9 2 Site Requirements and Specifications 13 Site Requirements 14 Physical Specifications 17 Performance Specifications 18 3 Installing the Degasser 19 Unpacking the Vacuum Degasser 20 Optimizing the Stack Configuration 21 Installation Information on Leak and Waste Handling 24 Installing the Vacuum Degasser 28 Flow Connections to the Vacuum Degasser 31 Operational Hints for the Vacuum Degasser 34 Transporting the Vacuum Degasser 37 4 Usingthe Degasser 39 Leak and Waste Handling 40 When to Use a Vacuum Degasser 41 Prevent Blocking of Solvent Filters 42 Solvent Information 43 5 Optimizing Performance 49 Increasing the Degasser Performance and Degassing Level 50 6 Troubleshooting and Diagnostics 51 Overview of the Degasser s Indicators 52 Status Indicators 53 Module Status Indicator 54 Hardware Symptoms 5
47. nish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 92 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual 11 Appendix General Safety Information 94 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 97 Radio Interference 98 Sound Emission 99 Agilent Technologies on Internet 100 This chapter provides addition information on safety legal and web ee Agilent Technologies 93 11 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is
48. ocess removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si0 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended 1260 Infinity High Performance Degasser User Manual Using the Degasser 4 Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyan
49. on location depends on detector Figure 4 Recommended Stack Configuration for 1260 Infinity Rear View 1260 Infinity High Performance Degasser User Manual 23 3 Installing the Degasser Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security concepts are understood and instructions are carefully followed WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet gt Do not use bottles that exceed the maximum permissible volume as specified in the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets gt Arrange the bottles as specified in the usage guideline for the solvent cabinet gt A printed copy of the guideline has been shipped with the solvent cabinet electronic copies are available on the Internet Recommendations for Solvent Cabinet Fo
50. or HPLC applications 1260 Infinity High Performance Degasser User Manual 47 4 Using the Degasser Solvent Information 48 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual e Optimizing Performance J Increasing the Degasser Performance and Degassing Level 50 This chapter gives hints on how to optimize the performance or use additional devices a Agilent Technologies 49 5 Optimizing Performance Increasing the Degasser Performance and Degassing Level For some critical applications it might be useful to increase the degasser s performance and degassing level by using two channels of the degasser in series with only one solvent channel of the pump For doing so 1 Connect the solvent inlet tubing bottle head assembly Bottle head assembly G1311 60003 coming from the solvent bottle to the inlet line of the first channel you want to use 2 Connect the outlet of the first channel to the inlet if the 2nd solvent channel of the degasser with the help of the short connecting tubing p n G1379 68706 delivered with the accessory kit of the degasser 3 Connect to the outlet of the 2nd channel of the degasser and into the pump from bottle to pump Figure 11 Connecting two degasser channels in series 50 1260 Infinity High Performance Degasser User Manual 1260 Infinity High Performance Degasser User Manual 6 Troubles
51. ors p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground ae NC 2 Brown Prepare run Low o i 3 3 Gray Start Low z NC 4 Blue Shut down Low a NC 5 Pink Not Ls connected Se NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Infinity High Performance Degasser User Manua 75 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 1260 Infinity High Performance Degasser User Manual BCD Cables Identifying Cables 9 One end of these cables p
52. r details see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets 24 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 Installation Information on Leak and Waste Handling Figure 5 Leak and waste handling overview typical stack configuration as an example 1260 Infinity High Performance Degasser User Manual 25 3 Installing the Degasser 1 Solvent cabinet Leak pan 3 Leak pan s outlet port A leak funnel B and corrugated waste tube C 4 Waste tube of the sampler s needle wash 5 Condense drain outlet of the autosampler cooler 6 Waste tube of the purge valve 7 Waste tube 1 Stack the modules according to the adequate stack configuration The leak pan outlet of the upper module must be vertically positioned above the leak tray of the lower module see Figure 5 on page 25 2 Connect data and power cables to the modules see section Installing the Module below 3 Connect capillaries and tubes to the modules see section Flow Connections to the module below or the relevant system manual Toxic flammable and hazardous solvents samples and reagents gt Keep solvent path free from blockages gt Keep the flow path closed in case the pump in the system is equipped with a passive inlet valve solvent may leak out due to hydrostatic pressure even if your instrument is off gt Avoid loops gt Tubes must not sag gt Do not bend tubes
53. r of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 14 1260 Infinity High Performance Degasser User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument
54. rovides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1260 Infinity High Performance Degasser User Manual 77 9 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 78 1260 Infinity High Performance Degasser User Manual Identifying Cables 9 CAN Cable Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair net
55. s the module status Status indicator SS Power supply indicator Figure 12 Location of Status Indicators 1260 Infinity High Performance Degasser User Manual 53 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of
56. sser 34 General Priming Instructions 34 Priming the Degasser with the Pump 35 Priming the Degasser with a Syringe only recommended if priming with the pump fails 36 Transporting the Vacuum Degasser 37 This chapter gives information about the preferred stack setup for your system and the installation of your module Ee Agilent Technologies 19 3 Installing the Degasser Unpacking the Vacuum Degasser Damaged Packaging CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist 20 Ensure all parts and materials have been delivered with your module The delivery checklist is shown below For parts identification please check the illustrated parts breakdown in Parts for Maintenance on page 65 Please report any missing or damaged parts to your local Agilent Technologies sales and service office Vacuum Degasser Delivery Checklist p n
57. t by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Infinity High Performance Degasser User Manual 95 11 Appendix Safety Symbols Table 7 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal
58. t non operating temperature 17 ambient operating temperature 1 7 analog signal 90 analog cable 72 APG remote connector 29 apgremote 91 BCD cable 77 bench space 16 C cable analog 72 BCD 77 CAN 79 29 external contact 80 LAN 79 power 55 remote 74 55 RS 232 81 cables analog 70 BCD 70 CAN 71 external contact 71 LAN 71 overview 70 remote 70 RS 232 71 CAN cable 29 CAN cable 79 changing solvents 34 36 cleaning 61 condensation 16 configuration one stack 22 22 control assembly 8 cover 66 D defect on arrival 20 delivery checklist 20 dimensions 17 E electrical connections descriptions of 85 electronic waste 97 error state 56 56 exchanging solenoid valve 63 external contact cable 80 F flow connections 31 31 flowrate 18 frequency range 17 1260 Infinity High Performance Degasser User Manual Index fuse 55 63 hardware symptoms 55 highest injection precision 41 highest retention time reproducibility 41 humidity 17 information on sound emission 99 installation bench space 16 interfaces 86 internet 100 introduction to the degasser 8 L lamps off degasser appears dead 55 status indicator is red 56 56 status indicator is yellow vacuum pump notrunning 56 LAN cable 79 line frequency 17 line voltage 17 M main cover assembling 62 materials in contact with solvent 18 maximum flowrate 18 maximum sensitivity 41 101 Index non operating altitude 17 non
59. te written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result
60. th sides Figure 9 Removing the Front Cover If the vacuum degasser is not used with an Agilent 1260 Infinity pump connect the waste tube from the accessory kit to the waste outlet and place into your waste system Put the bottle head assemblies into solvent bottles containing your mobile phase 1260 Infinity High Performance Degasser User Manual 31 3 Installing the Degasser Flow Connections to the Vacuum Degasser 5 Connect the solvent tubes from the bottle head assemblies to the inlet connectors A through D typically the left connection of the channel of the vacuum degasser Use the mounting tool for simplified holding and fixing of the tubing fitting Fix the tubes in the clips of the vacuum degasser Inlet connectors M moe ae Outlet connectors SS T NA JI A ET m e E e A e seed 2 Siew ES FF e ae IS Clips T it AA p 5 5 a rr E K 6 Connect the outlet tubes to the output ports typically right connection of the channel of the vacuum degasser Use the mounting tool to fix the tube screw 7 Prime the degasser before first use see Priming the Degasser with the Pump on page 35 NOTE Atmospheric gases can diffuse through the tubing and dissolve in the mobile phase solvents For best chromatographic results keep the length of tubing between the vacuum degasser and your pump as short as possible 32 1260 Infinity High Performance Degasser User Manual Installin
61. ty High Performance Degasser User Manual 29 3 Installing the Degasser Installing the Vacuum Degasser Status indicator Ef Waste outlet Power switch Module type and serial number Figure 8 Front of the Vacuum Degasser 5 Press in the power switch to turn ON the vacuum degasser NOTE The power switch stays pressed in and a green indicator lamp in the power switch is ON when the vacuum degasser is turned ON When the line power switch stands out and the green light is OFF the vacuum degasser is turned OFF 30 1260 Infinity High Performance Degasser User Manual Installing the Degasser 3 Flow Connections to the Vacuum Degasser Parts required Hardware required Preparations Description Solvent cabinet including solvent bottles filled with solvent and bottle head assemblies Solvent outlet tubes Syringe with adapter Degasser Install the degasser When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Put the solvent cabinet with the bottle s on top of the vacuum degasser Remove the front cover by pressing the snap fasteners on bo
62. urn unwanted products contact your local Agilent office or see www agilent com for more information 1260 Infinity High Performance Degasser User Manual 97 11 Appendix Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 98 1260 Infinity High Performance Degasser User Manual Appendix 11 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1260 Infinity High Performance Degasser User Manual 99 11 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com 100 1260 Infinity High Performance Degasser User Manual Index A accessory kit 68 Agilent oninternet 100 algae growth 42 ambien
63. work cable shielded 7 m for point to point connection 1260 Infinity High Performance Degasser User Manual 79 9 Identifying Cables External Contact Cable 5555 1 10 6 15 41 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 80 1260 Infinity High Performance Degasser User Manual Agilent Module to PC p n G1530 60600 RS232 61601 5181 1561 Identifying Cables Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m 1260 Infinity High Performance Degasser User Manual 81 9 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parall

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