User Manual - Hudson Robotics
Contents
1. oleja a ejej 11 m e altue TIX a eel viz Change plate types GetBottom y 1of1 Step 1 of 1 Get Well Bottom Search Params 2 Z Speed 5 Z Force 5 Amn Number of Wells to Search On Each Pass 1 ALL 7 Distance Above Well to Start Search mm o Select Output File Full Path jesu sers Phil Farrelly Documents Hudson Ro Browse Plate Barcode None lt the 1 hannel SOLOs use ONL O Warning for Multi Increment Order i Row Order 4142 Column Order 41 B1 47 The user should also confirm the Search Params as percents of full speed and force at which the probe tip will descend to the surface being measured The user must then select the number of wells to search entering 1 to select all unblocked wells and the distance above the plate to begin the actual search The user would then select the order for incrementing the probe from one well to the next Row or Column order and may block out individual wells of entire rows or columns by clicking on the plate graphic at those wells or rows columns Those wells thus appearing in BLACK will not be measured It is then up to the user to actually use the file data this step produces such as in a special VB step in SoftLinx or another external software application The saved measurement is the detected height of the well bottom above th2. arranging the steps in the order desired by the user As each step is dropped into the white field the grey area to the right will provide a GUI specific to that type of step that allows the user to define parameters for that step A completed method will consist of a series of steps arranged in a single vertical column much like a flow chart Step types available to the user are described below 28 3 2 Aspirate Step t This step will move the pipettor to a selected aspirate position then aspirate the indicated volume of fluid The display shown below depicts an Aspirate step drawing 95 uL from well A3 of a 96 well plate located at Position on the SOLO s deck on its first execution then 75 uL from well C4 on its second execution see the LOOP step below 0 Hudson s ni 1 Mi Fie Edit Action View Tools Window Help Djela a x mej l el Slep 1 of 1 alot RIE Y El A F p 5 Pisng Aspirate IZ tirdBotlom tVessel 5 3 Foc hiap MinCyclox 3 amp 811 w yji lic vall nse Shit above Jotton iv Sab p nia FE Mu oluma f 30 uL E _ gt S at ay Emptying yee 4 E change plate types Corning 3367 15 Same obore BOTTON IC J 5 Syring gt Spose Pic Aspirate Delay Ater Dispense 1 0 Sec fo gt fo u 100 0 1 Dwell Ater Aspra e 2 sec Bapirato Z 4 6 b T 1 of I HRE Aspia e Shill anuants ae measued uu Pa ul Ihe loca icn ot the 14ULH 1 FUSIII
3. either to run it or to edit it The default storage folder for existing methods is C Program Files SOLOSoft however a browse window allows the user to for a method file that may be located elsewhere Multiple methods may be open at the same time with the currently active method being the one whose display is out front Close This will close the currently active method the one in front only Close All This will close ALL open methods 18 Save This will save the currently active method only using the path and name shown along the title bar of that method Save As This allows the user to select a different path and or name for the currently active method This new path name will then appear in its title bar Print This will send the currently active method to the user s printer in a text format Exit This selection will close SOLOSoft 2 3 View Menu Toolbar Checking this option displays the Toolbar buttons along the upper left area of the Main Screen immediately below the Menu Bar Unchecking this hides these Status Bar Checking this option displays the status bar across the bottom of the Main Screen Unchecking it hides this bar Enable Logging Checking this option will establish a Method Log File for each method that is run Every step executed by the method will have its date and time of completion logged along with process data applicable to that step if any The log
4. s well depth as listed in its definition see Define Plate Types above 1s the limit for this setting and the distance above the plate to begin the actual search The user would then select the order for incrementing the probe from one well to the next Row or Column order and may block out individual wells of entire rows or columns by clicking on the plate graphic at those wells or rows columns Those wells thus appearing in BLACK will not be measured It is then up to the user to actually use the file data this step produces such as in a special VB step in SoftLinx or another external software application One line of comma separated text data is written to the file per well as follows Format Date Time Plate Barcode Well ID Liquid Depth mm Example 9 28 2011 12 33 40 PM ABC123456 A03 7 03 46 3 13 GetBottom Step The GetBottom step allows the user to detect and record in a file the level of the bottoms of selected wells in a microplate located at one of the SOLO s six plate nests The user begins by selecting the plate nest and its type of plate where the microplate being measured will be located Next using the Browse button depicted in this step s display below the user selects the location and name of the file where the well bottom data will be saved OLO SOLOSoft CAUsers Phil Farrelly Documents Hudson Robotics JENA File Edit Action View Tools Window Help
5. GetLevel Step LI The GetLevel step allows the user to detect and record in a file the liquid level inside selected wells in a microplate located at one of the SOLO s six plate nests The user begins by selecting the plate nest and its type of plate where the microplate being measured will be located Next using the Browse button depicted in this step s display below the user selects the location and name of the file where the liquid level data will be saved e File Edit Action View Tools Window Help Carle S ejej liiimj e ajaja h T X A Step 1 of 1 O e II w M z Costar 96 Search Speeds Mi La Change plate types Z Axis 5 Syringe 2 Maa GetLevel 1 off Number of Wells to Search On Each Pass 1 ALL Eg Maximum Search Depth into Each Well mm 10 Distance Above Well to Start Level Search mm 1 Select Output File Full Path C Documents and Settings Phil Farrelly My D Browse Plate Barcode None r Increment Order Row Order 41 42 Column Order 41 B1 The user should also select the Search Speeds as percents of full speed at which the probe tip will descend to the liquid surface and the speed at which the syringe piston will expel air until the surface is reached The user must then 45 select the number of wells to search entering 1 to select all unblocked wells the depth to which the probe should travel before stopping that plate
6. Micro l OX A dei cli oh Arde as A o dd S 51 A SAA e Sosa odd dA 51 E Shaker NCSU eo a OB dsv oda r E dd go oti aida 52 5 154 Wait For Input seer a A T dg 52 BS A NI n a ad a i A 53 LP HOUE OI Nest Ai 53 Introduction The SOLO Robotic Pipettor is designed to easily automate complex pipetting tasks that would otherwise prove tedious or error prone for researchers to perform manually The SOLO can be controlled as a stand alone instrument using its easy to learn user software called SOLOSoft Or it can be integrated into larger automated systems using its own Active X control Hudson s SoftLinx scheduling and workcell control software offers such an integrated interface In its standard configuration the SOLO offers 4 plate nest positions for locating labware such as microplates disposable tip boxes fluid reservoirs or other SBS standard labware Two additional locations can be added to either the left or right side ofthe SOLO to allow a total of six nest locations Mounted on the side is a flush station with a drain upon which is mounted a forked fixture for removing disposable tips An emergency E Stop button is mounted on the front body ofthe SOLO Pressing this immediately stops all motion of the major X Y Z axes and causes the normally blue pilot light located on the upper arm to turn red While the E Stop button is pressed in arm power is removed from the major axes allowing them to be manually moved by the user We curr
7. aspiration will increment in the order Al A2 A12 Bl B12 etc Column Order disabled for multi tip option of P12 type syringe Al Bl H1 A2 H2 etc Reverse Order wells with non zero volumes are selected beginning at H12 for a 96 well plate instead of A1 Row or Column order rules apply but in reverse Note For multi tip P8 syringe all non zero volume wells in each row will be addressed before moving to the next row Note For multi tip P12 syringe all non zero volume wells in each column will be addressed before moving to the next column Any wells with a zero volume will be skipped by single tip units and not included in the increment count Syringe uL This is read from the SOLO s firmware and is selected from a dropdown list of the syringes actually on this unit Start by Emptying Syringe Pn syringe type only where n is 0 1 8 or 12 Checking this box will cause the needle or tip to move over the intended aspirate position then the syringe piston will move to its bottom empty position at the start of this step This is useful to ensure that the full capacity of the syringe is available for this aspiration Otherwise the aspiration will start at the last position of the syringe piston Find Bottom of Vessel or Well If this box is checked the tip will travel down under low power until it stalls on the bottom of the plate well or tube Then it will rise b
8. file is saved in the same folder as the SOLOSoft application and has the file name MethodName YYYY MM DD HH mm SS log where MethodName is without the hso extension and the date time in the name refers to the moment that method is started Refresh This refreshes the display of currently opened methods 19 2 4 Tools Menu Simulated Configuration This option is only active when the communications port see Communications below is set to lt none gt forcing SOLOSoft into Simulate Mode This allows the user to try out different SOLO configurations such as switching between P D P8 and P12 syringe configurations changing syringe volume and switching from Landscape to Portrait layout Type of Syringe C Pipettor C Pipettor Dispenser Volume Number of Positions 1000 uL 4 Nests Deck Alignment Landscape Portrait OK System Liquid not applicable to simulation mode a P Syringe Type Z axis mounted syringe This option allow the user to move the syringe piston Up Aspirate or Down Dispense where only the syringe piston moves not the robot axes Aspirate Volume uL FILL Speed 2 100 Syringe uL Start Aspirate the user can enter a volume uL or by leaving the volume at FILL run the syringe piston all the way to the top of its stroke Dispense the user can enter a volume uL or by leaving the volume at EMP
9. 26 134 142 55 63 71 79 87 95 103 111 119 127 135 143 56 64 72 80 88 96 104 112 120 128 136 144 57 65 73 81 89 97 105 113 121 129 137 145 31 The file for a 384 well plate would have 16 rows of 24 values each For multi tip operation only values for wells addressed by first tip of multi tip head are taking in consideration Tips on the head are counted from left to right See bellow file list for aspirating with 12 tip head P12 from 96 well plate 50 50 50 50 50 50 50 50 50 50 50 50 42 42 42 42 42 42 42 42 42 42 42 42 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 20 20 20 20 20 20 20 20 20 20 20 20 10 10 10 10 10 10 10 10 10 10 10 10 Well positions that have zero or negative volume values will simply be skipped The SOLO will aspirate from the number of non zero wells determined by the Multiple Wells setting then if inside a Loop will start at the well following the last one aspirated during the previous execution of this step The volumes to be aspirated will appear in their respective well locations in the plate image across the bottom of the display Aspirate from Named Point The volume to be aspirated from a point such as a vial location can also be imported from a comma separated text file but for this type of position the values can be in any number of ro
10. SOLO User Manual Version 6 0 Aludson ROBOTICS INC 10 Stern Avenue Springfield NJ 07081 Tel 973 376 7400 Fax 973 376 8265 Intradu non isisao aaa A e ka iaai 3 Section Hardware rie i aia 4 leUinpacking the SOLO o as a aj na 4 2 E SI ANA A S SN REGRESIJA 7 Dl ek Pump tia dci 7 2 2 Hamilton PUM A RA A EI ad RASU 8 2 3 Multichannel A A ETA 9 CCSS A GAR vases 11 Section 3 1 Heating Cooling NES azec osin ls volan cove nerve s adi 11 Section 3 3 SOLOP US aia das 12 4 Hardware Specifications hd A E aa 15 SECOND BOLEWATE i sosa sdait esasrastua 17 WS TiS tat AHO ass o e fon steals od dsl o AA DN odo o odd aE 17 Pa sta ED o o ter On ooo da SA das 17 I SRD 232 0 E Re ud KRA i da pei sj 17 2 BOLOSOTE Main SCT asa sisaala dt aii 18 puk ido g mn re mer 18 A O ANN 18 NO 19 ZA Tools MENU iia 20 2 9 Window Help Men s siii dict 27 3 Developing SOLOSoft Methods ii AA da ete 28 BS OVV E Waerea a O BA 28 3 2 A Spitate STEP iako oE E E a a o s 29 5 Dispense SED a aaa io ak a nd dlan 35 A O S SDA A Er 36 3 3 BMICE Tip stopira an e jeo akata 37 3 6 Loop End Loop EOS Ao 38 De Pause PI IA a a e vida 38 O MON AO a R 39 A o SED OD GE ER E RE ER SSS 40 510 Prime Sepia in 41 3 11 HitPick Step available ONLY for single tip operation modeS 42 De ee Ss ka A to ges o ka 45 Sel O teb abadi A EE Ones 47 Sel Chance Plate TP ds 49 3 15 Operate AGCESSOTY rt ia 50 S RSS
11. Syringe 868 il Aar u Priming Position Flush gt i gt Syringe Speed 100 Change Plate Types Dispenser Pipettor m Syringe uL Valve Port 1000 2 PRIME C EVACUATE Total Volume uL e TotalNo Strokes 1 mr S sheet ogether A SOLO can have multiple D type syringe pumps up to 10 and this step allows any individual syringe pump to be exrcised or all at once If all are exercised at once the Total Volume selection is greyed out and only the Strokes selection is allowed Also the valve port selection must be the same for all The display for a SOLO equipped with s type P syringe appears as follows 41 C Document 1 4 t j s Y X A Prime Syringe fon es il y u Priming Position Flush v Syringe Speed 100 gd Change Plate Types Pipettor FILL Syringe EMPTY Syringe Aspirate Volume Dispense Volume The user make elect to position the syringe piston at the very top of its stroke FILL or position it at the bottom of its stroke EMPTY Alternatively the syringe may be moved by a selected amount in uL either up Aspirate or down Dispense 3 11 HitPick Step available ONLY for single tip operation modes le The HitPick Step provides the user with a convenient way to consolidate the contents of selected wells from one or more source plates into one or more target plates This step can be use
12. TY completely empty the syringe by running the syringe piston all the way to the bottom of its stroke 20 b D Syringe Type Side mounted valved syringes This option allow the user to move liquid from any of the systems fluid reservoir vessels to or from the tip For both Aspirate and Dispense the reservoir is selected by selecting the appropriate Valve Port in the displayed input box Aspirate Volume ul 0 Speed 2 100 Syringe uL Valve Port la 125 y 2 Dperation 7 Continuous Start No Strokes EE Aspirate the user can enter a volume uL to move from the tip to the reservoir even volumes greater than the syringe s volume or by checking the Continuous operation box run the syringe piston continuously until the user clicks on the Halt button The No Strokes box can be checked and the desired number of aspiration strokes entered as another operation option If there are multiple syringe pumps on the SOLO the Syringe box will drop down to allow the user to select any individual syringe or at the bottom of the list ALL syringes operating together Dispense the user can enter a volume uL to move from a reservoir to the tip even volumes greater than the syringe s volume or by checking the Continuous operation box run the syringe piston continuously until the user clicks on the Halt button The No Strokes box can be checked and the des
13. UN Multpe wels lif a Plats 2 Shit is rom BOTTOM of WELL E E pas ve mi I Do Tip Touca SSM SEE ee pros lo Aspiae Sailt Inn Tip cuch Shit rem Aspirate fror Plate Fosition pia X Shit To X thit EE Aspirate frorr Named Poirt z voki Chft pi sm st F o ao EE nerement Orcer ZABE Je sl o RowCrde A1A2 Switch to File Data Cine lore Pont Celunn Order 41 81 Asprate Volancs u a wo u e The user has a number of parameters to select in an Aspirate step Select Type of Source Plate Position A Plate Position can be selected and identified by clicking on one of the 4 possible deck plate nest positions Position1 to Position4 in the deck image above the selection box Named Point if this is selected the screen will change to that shown at the end of this subsection below allowing the user to select ANY taught position whether or not associated with a plate nest The available points are selected from a drop down list shown near the center of the display and the user would enter the desired volume in the box immediately above this 29 Increment Order applies only to aspirating from a plate position for SOLO equipped with single tip pipettor head or multi tip pipettor head used in Single Tip Mode Row Order disabled for multi tip option of P8 type syringe Ifa multiple number of wells per execution is entered or if this step is within a Loop each
14. arcode None I arget Plate Barcode None 01234535 78 9101112151415161718132021222324 ASAS A O E A PREBRBERRSTEH mamajananjuniujm QOOOO000I00 aoran EN Ela Go mi omg maaaananananja 000 odo 000 O00 000 O00 non oog onn oog O00 O00 ooo Oooo 30 300000 OOOO 303000 wk OOOO AD IOOODOOO o im o O o LI O A oO m m j TOZTTACIOAMO os PODOJOJ0000l PODOJO 0000 EndLoop 7 of 7 COCON DOOM Increment Order oe 2 Stent by Emptying Syinge Row Order 41 42 Column Orcer 41 61 1 Syringe Speed PreAspiate Backlash Syinge Speed Blow off Bac lt lash y lie gt The user can select a Maximum Volume per Aspiration to allow the use of pipette tips that may be smaller than the amount needed to complete the transfer and may set the number of Target Wells per Source Well The SOLO will only aspirate up to the Max Volume each time If more than one Target Well is to be filled per Source Well the SOLO will divide that maximum volume by the number of 43 target wells and dispense equal amounts in each until they receive the full amount required In order to reduce any effect from pipetting residual volumes at the very lowest end of the pipette tip s range the next to last aspiration step will be limited to 50 of the total remaining aspiration volume so that the last aspiration is also at least 50
15. aspirations done in each execution of this step in the selected row or column order Any wells with a zero volume will be skipped and not counted in the number selected Note For multi tip P8 only column order is available and for P12 syringe only row order is available Switch to File Data Ifthe Switch to File Data option is selected the user will be shown a browse screen to browse for the file or to just enter its name The selected file and its path will be shown just below the button s location The text on the button will change to Switch to Entered Data to enable the user to go back to using the plate image table across the bottom of the display and manually enter volume values for each well or the named point The file structure will be determined by which type of position is to be aspirated from Aspirate from Plate Position The volume to be aspirated from each well can be imported from a comma separated text file with a row column format that matches the plate type selected for this position e g 8 rows of 12 values each for a 96 well plate This file lists each row on a separated line with each column s value separated by a comma resulting in a file similar to that shown below for a 96 well plate 50 58 66 74 82 90 98 106 114 122 130 138 51 59 67 75 83 91 99 107 115 123 131 139 52 60 68 76 84 92 100 108 116 124 132 140 53 61 69 77 85 93 101 109 117 125 133 141 54 62 70 78 86 94 102 110 118 1
16. d for cherry picking rearraying and re formatting from one size plate to another The HitPick step is setup much as if it were an Aspirate Step and a Dispense Step combined into one The user chooses the syringe selection syringe speed X Y Z offsets and Tip Touching are similar to those in the Aspirate and Dispense Steps described above and are not repeated here The user must select the Source plate s position out of the available plate nest positions 1 through 6 and the target plate s position These selections will also define the type of plate that will be present at each of these positions while running just as in the Aspirate or Dispense Steps above The major differences between the HitPick Step and the Aspirate or Dispense Steps are a The Source plate s data ALWAYS comes from a CSV file for which the user selects the folder where the source file s will be found b The Target plate s information is derived from a blocking pattern that is stored with the method c The user selects an Output file and file path where the transfer information is to be stored the same file for all plates in a run There must be a separate source file for each Source plate where that files name is SourcePlateBarcode csv For instance if that Source plate s barcode is 42 ABC123 then the file s name would be ABC123 csv The file contents are simply the well name of each source well followed by a comma then the am
17. e DeckHeight of that plate nest The data is written to the file per well as follows Format Date Time Plate Barcode Well ID BottomHeight mm Example 9 28 2011 12 33 40 PM ABC123456 A03 3 03 48 3 14 Change Plate Types This utility allows the user to select the type of microplate or tip box omni tray etc that is located in any of the 4 plate nest positions It also allows the operator to reset any used tip box count to zero which would have the SOLO start retrieving tips at the A1 tip position th Select Plate Type Select Deck Layout Position Posiion2 y Position1 Position Position2 Plate Type Position2H ositiond My384 s Identify the TYPE of PLATE at any of the late Positions To change a plate type at any of the 4 positions or to add a plate where the nest was empty click on the down arrow of the box for that position and a list of all the defined plate types in the SOLOSoft database will be shown Clicking on the desired plate will add it to that position The OK button will save the changes while the Cancel button will restore the original data These changes will take effect immediately and remain so while this method is loaded In order to preserve these changes the next time this method is loaded the method itself must be saved upon closing it 49 3 15 Operate Accessory A This utility allows the user to operate any of several o
18. een left on the mandrel from a prior activity then move to the next tip position in a tip box located at in this example Position1 6 File Edit Action View Tools Window Help plela a jajaj 0 m 2 aerfa 1 TX Al Step 1 of 1 TipBox 200uL Select Disposal Point 60 li de ik Bl TipDisposal Change plate types Syringe Type Single Channel Number of Tips to Use q 4600000000000 000000000000 000000900000 Refill TipB ox 0600000000000 000000000000 The location of the tip box is selected by clicking on the appropriate nest position in the displayed deck image At the start of this step the SOLO will attempt to shuck the tip or tips in case the tips were left on the mandrel from a prior operation Ifthe position selected in the Select Disposal Point box is the same as the plate nest where the tip box is located the tips will be shucked into the last box position emptied However if a method was just loaded if the tip box is full or if a multi tip unit is being used with fewer than its maximum tip count 1 e 8 or 12 then the tip shuck will occur at the position in the Select Disposal Point if it is different from the location of this tip box or at the Tip Disposal position by default For all syringe types SOLOSoft keeps track of tip count in tip boxes between methods and even if closed and powered down For single tip units the tip retrieved in this step will be the next tip i
19. ently carry three configurations of the SOLO chassis The standard configuration includes a either a 125 uL or 250 uL capacity syringe that is mounted on the robot s vertical axis offering individual pipetting volumes from 100 nL to 250 uL and a waste station and a tip shucking station to automatically remove disposable pipette tips The second configuration offers a syringe pump mounted on the rear vertical frame member with either 8 or 3 position valves The syringe is mounted on the pump body and may be easily changed by the user to sizes ranging from 25uL to 10mL per full stroke This configuration allows the SOLO to perform as both a Pipettor and Dispenser drawing fluid from any one of up to 7 fluid reservoir bottles The Third configuration offers either an 8 or 12 channel multitip pipettor The 8 and 12 channel heads come in either 10ul or 200ul syringe capacity per channel configurations The multitip head can be pick up anywhere from one to the maximum number of 8 or 12 tips The SOLO also offers an alternate configuration that allows it to become a component in Hudson s LabLinx track based stacker system providing automated feeding and removal of plates and or tip boxes for high throughput applications The LabLinx track mounts directly to the SOLO s deck taking the place of 2 or 4 plate nest positions or even all 6 if very high throughput is desired The SOLOPlus is a SOLO integrated with Hudson s Micro10x high speed bul
20. figure allows the user to simply move the robot arm to any taught position at a speed given as a percentage of the arm s full speed in the XYZ Speed box The X and Y axes move first followed by the Z axis 39 C Document 1 Sale sues Y XK AJ Move Arm top li Ar t Destination TipDisposal y Vv Move Z Axis to Travel Height at Start of Move Change Plate Types Move rm 1 of 1 XYZ Speed Foo As an option by checking the Move Z Axis box the tip will move up to its normal travel height prior to moving laterally to the selected destination position 3 9 Set Speed Step m The default robot arm speed is 100 of full speed This step shown in the next figure allows the user to set the movement speed for subsequent steps at the percentage of full speed depicted in the XYZ Speed box This will be overridden by the value set in any Move Arm step that may follow E Document 1 DER E LL or 13 Set Arm Speed Change Plate Types XYZ Speed Foo 2 Vv SetSpeed 1 of 1 40 3 10 Prime Step L om The Prime step allows a user to prime or evacuate D type syringe liquid lines or to position a P type syringe piston at a desired position at the start of a method or even at intermediate places in a method The display for a SOLO equipped with a D type syringe appears as follows FS Document 1 4 t j Y X Al Prime
21. hoose to use the included RS 232 cable to connect their computer to the SOLO If you are using RS 232 a 9 pin female RS 232 connector is located on the rear panel of the SOLO Connect the provided cable here and either to the serial port on your computer or to a USB to serial converter Selecting the serial port assignment and communication parameters is covered in another section of this manual 17 2 SOLOSoft Main Screen 2 1 Overview Upon Startup SOLOSoft presents the Log In display which shows the current Version and a Log In button Click on the button then enter your name and password On initial usage the default User Name is User with a blank password Under the Tools Menu discussion below are instructions about setting up actual user names and their passwords After entering a valid user name and password the Main Screen display is shown as below SoloSoft File view Tools Window Help olsa S lees 2 Clicking on any of the five options on the Menu Bar provides the menu features described in the following subsections 2 2 File Menu New This option creates a blank method whose default name is Document1 and the user can now start developing a new method as described in the Method Development section below The new method can then be saved under the Save As option described below Open This option allows the user to open an existing method
22. ill be taken sequentially from the first available position along rows starting with row H and progressing in direction from column 12 to 1 and then rows H to A In case of using multi tip option tips will be picked up from the first row with enough available tips starting with row H For guidance on programming tip pickup positions and using different length disposable tips in different methods see the subsection below entitled Programming with Disposable Tips 3 5 Shuck Tip Step X This step whose GUI is shown in the next figure allows the user to discard a disposable tip at the position indicated in the Select Disposal Point box This is usually used at the very end of a method to strip off a remaining tip Otherwise the Get Tip step is normally used to shuck a tip prior to getting another FS Document 1 alere Y XK a Shuck Disposable Tip CK I y v Select Disposal Point TipDisposal v Change Plate Types INE ShuckTip 1 of 1 37 3 6 Loop End Loop Steps 80 These steps shown in the next figure at the top and bottom of the method flow diagram allow the user to repeat sequences of steps the number of times indicated in the Enter NUMBER of Loops to Perform box After repeating every step between these two loop steps the method will continue with the next step after the End Loop step Loops can be nested within other loops If for instance a loop with 3 repea
23. inn or each paso X Shif KE X Shit i Aspi alef Newer Purl 1 shi Po Y Shrt co Incramea O der a 2 ze an gt aqaa Swilrh m Fila Mata Fostiva Z is Zaft from UE I tike nar amp gIrat Point E Cukir Oda A1 E1 When using liquid level detection the Z shift value in the Aspirate Shift box indicates the distance to move the tip below the detected liquid level prior to aspirating If the tip reaches the Max Depth position without detecting liquid the Aspirate step will end and SOLOSoft will perform its next action 34 3 3 Dispense Step el This step will move the pipettor to a selected dispense position then dispense the indicated volume of fluid The display shown below depicts a Dispense step expelling 95 uL into well C2 of a 384 well plate located at Position on the SOLO s deck on its first execution then 75 uL into well C6 on its second execution The parameter selections are identical to those of the Aspirate step except for the following Mixing Same as Aspirate step except in opposite order but ends with a dispense as well Blow off This amount will be expelled from the syringe after the last dispense amount in this step is released It is used to blow off a droplets that may be on the tip at the end of dispensing T tie Eat Acton ew Toos indow Hep Dla el sele ulaj 9 ES TAK Al E Mixing DO eu mispensas pr Maat Nit Cooles 3 J
24. ired number of dispensing strokes entered as another operation option 21 Calibrate Deck This option allows the user to create teach save or delete robot positions within the working area of the SOLO s 3 axis robot arm as well as to jog the arm within that work area Clicking this option will produce the following screen display Deck Calibration Select a Position to Calibrate Position Name Jog Robot Left Right Current Location steps Position1 XAris EE XAsis 0 e 0 ro Position Coordinates steps Y Axis 5 T a Tom 2 Speen X Axis DB Up Down Z Axis 0 osition a Position6 di lt TI Save Point TipDisposal YAris 0 at Flush Units Current Location Z Axis 0 gt 5 fe mm C steps Set Plate Skew Move To Positi inat Delete Selected Position A Home Change Speed Relax Motors Add New Position ton Positions should be programmed with the needle tip or disposable pipette tip at the very top surface of the labware being addressed The first 8 position names in the list are PERMANENT and cannot be deleted however the user can re locate them anywhere within the work area of the robot however they are intended to be located properly for use in the SOLO s plate nests and tip shucking flushing stations as follows Position the left hand rear plate nest location It should be programmed with a disposable tip pressed onto the syringe tip mandrel in the case of a SOLO with this option or usi
25. k dispenser The dispenser is attached to the side of the SOLO with provided hardware The Micro10x and SOLO share a nest position and SOLOSoft seamlessly controls both instruments through its interface Section 1 Hardware 1 Unpacking the SOLO e Remove all material from inside the crate except for the SOLO itself e Open the Black nylon strapping that secures the SOLO to the shipping form pagana so naa i STAKLENA NEE ss gt Y 4 y uma 9 d E Me e FA AAA zak MU Shipping Straps e Once the straps are set aside lift the SOLO by the aluminum bracket that the nest plate holders are secured to DO NOT LIFT BY THE ARM as this will damage the unit See Picture on next page 5 Lifting Points on Solo e Once the unit is out of the crate remove all the plastic wrapping that secures the arm to the SOLO Remove Plastic Wrap PEE e Once the plastic wrap is removed install the nest plates on the deck of the SOLO Align the holes in the bottom of the nest plate with the metal dowel pins on the deck assembly and push the nest plates onto the deck e Remove the Power cable and communication cable from their shipping packages and plug them into the correct locations on the SOLO s rear panel e Install the SOLOSoft on the computer you are going to use with the SOLO e The unit is now ready to startup 2 SOLO Setup Depending on the version of SOLO that you purchased
26. l Sarge tt Finish Mix Volume 10 ul yrnge ul Y rt A Aspirate Height Change plate types Eicon 33224 15 lt z above BOTTOM 2 al 1 Eg 5 Syringe Speed r Blow off Delay After Aspirate 1 0 sec well After Dispense 0 sec hoz FO puetatebi e Aspirate 2 4 6 Backlash 1of2 Dispense Shift amounts are measured from 0 ul the location of the TAUGHT POSITION Multipl e Wells if a Plate Z Shift is from BOTTOM of WELL Enter the E Do Tip Touch Dispense Select Type of Target number of Dispense Shift mm s Tip Touch Shift mm 2 of 2 ini M dispenses to A Dispense to Plate Position pain on X Shift o X Shift 1 0 E y oe each pass Dispense to Named Point Y Shift ra Y Shift a Increment Order Z Shift 9 Z Shift o C Row Order A1 A2 1 3 7 Positive Z is Shift from Column Order 41 81 Switch to File Data Upwards Dispense Point Reverse Order Dispense Volumes uL ocooocoocooocooooooooo ococoocoocococomnoo ococoocoococoocomYoo ococoococoocoocooooo ocoocoocoocoocoocoooo ococoocooocooocooooooo ocoocoocoocoocooooooo ococoocooocooooooooo 20000000000000000o0 20000000000000000o0 2000050000000000000o0 20000000000000000o0 2000000000000000o00o0 olo0ooooooooooooool 20000000000000000o0 20000000000000000o0 20005005000000000000o0 35 3 4 Get Tip Step T This step whose GUI is shown in the next figure will start by shucking any tip that may have b
27. mn enter the depth of the bottom of the plate s wells measured from its top surface This Depth will be limit below which the SOLO needle or disposable tip will not travel when aspirating or dispensing within a SOLOSoft method See image below When defining disposable tip box parameters the Height value should be the height of the top of the disposable tip as it rests in the tip box above the surface of the plate nest The 4th column will contain the Tip Length that is the length mm of the disposable tip itself See image below Measurements in mm Tip Box Height Plate Height Max Depth This data is saved in an ACCESS database table C Program Files SOLOSoft SOLOSoft mdb This must be opened directly if the user wishes to delete a plate type 24 Communications This enables the user to select the serial port to be used by the computer to control the SOLO A drop down list of available serial ports will be displayed by clicking on the down arrow in the list box shown below Communication Setup 10 Way Valve 4 Way Valve Terminal Window SOLO Port Communications Port ICOM 1 y Test Cancel Apply The Terminal Window button provides a HyperTerminal window which will use the Serial port selected in the Communications Port list box to enable the user to use the SOLO s ASCII Command Set see Appendix A The user can then issue commands directly to the SOLO f
28. mperature sec 53 The user would enter the desired temperature to be achieved the SetPoint in the appropriate box The range is 10 0 to 110 0 Deg C When a Method is running the Actual box will display the actual temperature of the Nest The user has several optional ways to execute this step e Turn Nest OFF at End of Method un checking this will allow the user to keep the heating cooling action running after the running Method ends If this remains checked it will turn OFF at the end of a run e Don t Wait this will send the SetPoint to the Nest starting heating cooling action but then will immediately continue to the next step in this method e Wait Until Actual SetPoint this will cause this step to hold here until the Nest s actual temperature reaches the targeted SetPoint then will immediately move on to the next method step e Wait Until Temperature is Stable this will cause this step to hold here until the actual temperature stops changing even though SetPoint may have been reached before this e Turn Nest OFF Now checking this will simply use this method step to turn the heating cooling action OFF then the step will exit e Turn Nest OFF at Temperature this will turn the nest OFF immediately upon satisfying either of the previous two selections then advance to the next method step e Log Temperature checking this box will cause this step to log the ACTUAL temperature to the SOLOSoft log file at i
29. n the box counted across the box in row order starting from the H12 or P24 well position For multi tip syringe types P8 and P12 SOLOSoft keeps track of tips available in tip box for both single tip and multi tip operations 36 The tip box will be reset to full if the type of box at that location is changed if the identity of that tip box is changed e g if operated using Hudson s SoftLinx scheduling software and a new tip box is moved into that position or 1f the tip box is reset in the Change Plate Types utility see that subsection below In the step s graphic plate image available tips are shown in GREEN For a single tip syringe type each tip will be taken sequentially from the first available position along rows starting with row H and progressing in direction from column 12 to 1 and then rows H to A for 384 well boxes substitute P for H and 24 for 12 For multi tip syringe type P8 utilizing single tip option the single tip will be taken sequentially from the first available position along columns starting with column 12 and row H progressing in direction from row H to A and then columns from 12 to 1 In case of using multi tip option tips will be picked up from first column with enough tips to meet the Number of Tips to Use entry starting with column 12 For a multi tip syringe type P12 utilizing single tip option the single tip w
30. ng the fixed tip It is intended to be programmed with a 384 well plate Align the tip between locations A1 A2 B1 and B2 This simulates the center of well Al ina 96 well plate located in that nest position A1 being located in the rear left corner of the plate nest Upon clicking the Save Point screen button SOLOSoft will save this point name with the current X and Y axis coordinates and set the Z coordinate equal to the Z axis current travel height setting This Z axis setting applies to all 4 or 6 for Portrait layout of the plate nest positions listed here Position2 the lefthand front plate nest position Position3 the middle rear plate nest position Position4 the middle front plate nest position Position5 the righthand rear plate nest position Position6 the righthand front plate nest position Flush located with the tip in or above the waste drain fixture 22 TipDisposal located where the syringe tip is within the tip stripping fixture with the forks of the fixture surrounding the syringe tip mandrel and just above the top of a disposable tip so that an upward movement of the robot will strip off the disposable tip The coordinates of any position selected from the list along the left will be shown in the box entitled Position Coordinates steps The user can directly modify these coordinates then move to the modified coordinates However the actual saved coordinate
31. nt bracket that is attaches the Micro10x to the SOLO chassis This alignment bracket can be mounted in the front position of the SOLO so that the Micro10x nest is located at the SOLO deck position 6 or in the back position so that the Micro10x nest is located at the SOLO deck position 5 This bracket contains two alignment pins which locate the Micro10x into its proper position During shipping the SOLO and Micro10x are shipped in different boxes To install the Micro10x onto the SOLO you will slide the Micro10x next to the SOLO where the alignment bracket is installed Slowly lift the Micro10x up so the bottom of the plastic deck is above the alignment pins Move the Micro10x into position so that the alignment holes in the Micro10x plastic deck are aligned with the alignment pins in the SOLO bracket Slowly move the Micro10x down until the Micro10x deck is located on the pins and that the bottom the Micro10x deck is sitting flush on the alignment bracket You may have to adjust the feet on Micro10x to re level the unit to the SOLO deck See Figure 3 4 112 MC10X Plastic Deck with Alignment holes SoloPlus Alignment Bracket Figure 3 3 13 i 07 Figure 3 4 4 Hardware Specifications SOLO Pipettor SOLO Pipettor Dispenser Hardware features Arm configuration One liquid handling arm Tip configuration 1 disposable tip Idisposable tip Pipetting Needle Tip variety Disposable tips
32. ntervals given by the entry in the accompanying box in seconds This logging will continue even after this step exits until the method ends 54
33. of the Max Volume setting The user can browse the computer for the appropriate folder where Source plate files will be found and can browse or simply enter the full path and name of the output file Then before saving the method can select those Target plate rows columns or individual wells which will be blocked from receiving transfers from the Source plate These will be colored black as in the above graphic The user can also select whether to fill the Target plate in Row across the top then down or Column order Pending but un aspirated hit wells on the Source plate are displayed in yellow while completed transfers are shown in green on both plates If the Source Plate s hits have all been transferred or if the Target Plate is full the HitPick Step will end and if this step is imbedded inside a Loop that Loop will end and the Method will proceed with the next step beyond that Loop s End Loop step Usually the Method will have been designed to end here to enable replacing either of the finished plates Not shown in the graphic above are the aspiration and dispensing settings that the HitPick Step has in common with the Aspirate and Dispense Steps which are in the lower portion of the HitPick Step s graphic Also a Mix option is provided for both the Source and Target plate wells which functions identically to those in the Aspirate and Dispense Steps described in those sections 44 3 12
34. ommunications utility All other SOLOSoft functions are available to Level 2 users User Level 3 All SOLOSoft functions are available to Level 3 users 23 Define Plate Types This enables the user to define the properties of the various microplates that will be used in the SOLO The following screen is displayed Plate Type No Plate Depth Tip Comments Wells Height Length OmniTray 96 15 15 PCR Plate 96 21 14 Demo Plate for PCR Serial Dilution Phenix Res RRI 3035 384 19 13 Narrow 16 Compartment Reservoir Reservoir NCI 96 44 38 Demo Plate for NCI Seahorse 50051 96 45 41 Reservoir 4 Compartment Tall SeaHorse 530018 96 19 3 148 Reservoir 96 Pyramidal low walls SeaHorse 530019 96 44 39 Reservoir 12 Compartment Tall Shallow Plate 96 20 5 TempPlate 96 15 12 TipBox 10uL 96 54 31 5 Axygen for Ivek TipBox 20uL 96 56 40 Axygen for Ivek TipBox 250uL 96 56 55 Axygen for Ivek TipBox 50uL 96 55 5 57 25 Axygen for Ivek TioBox FX250uL 96 56 52 Beckman FX Compatible Note For TipBoxes MaxDepth is Tip Length All Measurements are Millimeters Delete Plate Add New Plate Save and Exit The user creates a new plate by placing the mouse cursor in the last row of the Name column then typing in the name of the new plate The user must also enter the number of Wells the SOLO will calculate the center of each well based upon this the Height of the top of the plate from the nest surface then in the 4th colu
35. oning Precision X axis 0 025mm Y axis 0 025mm Z axis 0 004mm Motion Range X axis 594mm 23 3 8 Y axis 244mm 9 5 8 Z axis 101mm 4 Liquid handling features Pipetting M Bulk Dispensing N A No of fluid sources N A Syringe size 10 or 200 uL Wash Pump N A Safety features Password protection Emergency Stop E stop Button located on the front of the dispenser chassis Dimensions W 556mm 30 x D 483mm 19 x H 610mm 24 Weight 27 kg 60 Ib Power requirements Power 60VA Voltage 100 240VAC Frequency 50 60HZ Operating conditions Temperature 15 32 C 59 90 F Relative humidity 30 80 non condensing Regulatory compliance Computer requirements PC with Microsoft Windows XP or newer Quad Core 2 5 Ghz 3 Gigabytes free hard drive space RAM 4 Gigabytes 1 USB port 16 Section 2 Software 1 Installation 1 1 Installation CD Place the SOLOSoft Installation CD in your CD drive It will automatically begin the Installation process It will create a folder C Program Files SOLOSoft where the SOLOSoft executable files the SOLOSoft Active X control plate definition Access database and support files are loaded This folder is also the default folder for all SOLOSoft methods which have the file extension hso Several sample methods are loaded from the CD as well 1 2 RS 232 Most users will c
36. ount uL to be transferred into each target well The format would looks as follows A01 50 C09 75 each well volume entry is on a separate line H11 55 The user can use a header line if desired of Source Well for clarity This line is ignored by SOLOSoft if present The HitPick Step allows the user to fill multiple target wells from each source well and the amount aspirated from the source well will be that multiple of the amount in the source file That is if the user elects to fill 3 target wells from each source well the amount aspirated from well A01 above would be 150 uL with SOLOSoft multiplying the amount in the source file by 3 Each line in the output file has the format Date Time Source Plate Barcode Source Well Target Plate Barcode Target Well Amount uL with no header line A typical entry would appear as follows 6 23 2008 2 40 24 PM ABC123 A03 DEF456 A01 53 The picture below shows the HitPick Step s graphic image E C HudsonSOLOWocument 1 hso DER letjele TK 69 Source Plate Syringe u Target Plate es ae ee 125 8 li la Costar 96 Change Plate Types 3 5 a Shaker 2 4 5 3 a 7 Max Volume per No of Target Wells Agspiralion per Source Well 50 pul 2 e met Source Plate Aspiration Target Plate Dispensing Select Source File Folder Select Output File Full Path 4 PAHudsarS ALMA Browse PAHuds SA N HitRekNutnit ese Browse TP Gees Source Plate B
37. ptional accessories that are available for the SOLO Drag down the Operate Accessory button to select this type of step Then select the individual accessory by clicking on its icon button in the display shown below C HudsonSOLO SOLOSoft Document 1 6 File Edit Action View Tools Window Help oela S sele slm 9 AGIGEA YX al Step 1 of 1 BONI Operate Accessory Device Change plate types Select Device 2 Micro10x lA Accessory 1of1 E 10 Way Valve ENE Shaker Nest Wait For Input Write To Output os Hot Cold Nest EEE The individual accessory selected will be shown as described below 50 3 15 1 Micro10x Selecting the Micro10x action for this step will change this step s display to appear as follows als ll y XI A Step 1 of 1 BO x Operate Micro10x Change plate types Micro10x Dispense C Prime Empty A Accessory 1 of 1 Program 7 y This option allows a SOLOPlus to perform rapid dispensing from an attached Microl0x The user can select to run a dispense or prime program or to empty the fluid back into its source Dispense and prime programs are configured ahead of time in the Micro10x itself Consult the Micro10x manual for more information on creating and editing dispense and prime programs 3 15 2 Ten WayValve Selecting the Ten Way Valve action for this step will change this step s display to appear as follows als j 1X Al Step 1 of 1 Se vil
38. rations For the 12 channel the deck will be placed all the way forward towards the operator Figure 2 5 For the 8 channel head the deck will be placed back in the furthest position away from the operator Figure 2 6 Nests all the way back Nests all the way forward 10 3 Accessories The SOLO has several options that can be purchased in additional to the SOLO they are the following 1 Heating Cooling Nest 2 Shaker Nest 3 SOLOPlus SOLO w attached Micro10x Section 3 1 Heating Cooling Nest The Heating Cooling Nest requires the SOLO Accessory Platform Your SOLO will arrive with the SAP already attached Unpack the H C nest and place in the desired location deck location See Figure 3 1 Attach provided cables Figure 3 1 Section 3 2 Shaker Nest The Shaker Nest comes shipped separately from the SOLO The Shaker Nest has the same alignment holes that are located in the standard nest plates To install the Shaker Nest remove one the nest location and install the Shaker Nest in that location See Figure3 2 Plug in the supplied power communication cable into the Shaker Nest Plug 11 the communication cable into an available comport on the USB to serial converter Figure 3 2 Section 3 3 SOLOPlus The SOLOPlus option is the addition of a Hudson Robotics Inc Micro10x reagent dispenser that is attached to the side of the SOLO See Figure3 3 The SOLO is equipped with an alignme
39. rom the computer keyboard 25 Accessory Devices If the SOLO has the Shaker Nest Micro10 Hot Cold Nest 10 Way or 4 Way Valve Accessories the user would setup a serial port to control each accessory in the same manner as for the SOLO itself by clicking on the appropriate tab in the form shown above and selecting the desired COM port Each device also has 1ts own Terminal Window to allow the user to issue individual commands to it using its own ASCII command set 26 2 5 Window Help Menus The Window menu enables the user to manipulate the window display patterns familiar to users of MicroSoft Windows products The Help menu s About option will show the current version of SOLOSoft 27 3 Developing SOLOSoft Methods 3 1 Overview SOLOSoft enables the user to create save and run pipettor processes called methods A method consists of a series of sequential actions called steps that the SOLO will execute when that method is run Methods are created and modified using a drag and drop editor that is started when the user opens a new method or an existing one using the File Menu described above Using the File Menu to select the New option will display the following screen afere R all Gew lej E Lhange plate types The user adds each desired method step by dragging any of the Step buttons in the toolbar at the upper left into the white field immediately below
40. s of that position will NOT be overwritten unless the user clicks on the Save Point at Current Location button on the right The screen button Set Skew allows the user to adjust the calculated locations Of plate wells in case an individual nest is not in perfect alignment with the X or Y axes Pressing this button when teaching a plate nest position will provide the user with instructions on how to make this adjustment This is important particularly when dealing with 1536 well plates The screen button Relax Motors can be used to remove power from all 3 robot axes allowing the user to manually maneuver the tip to the desired location Clicking on that button again will restore motor power Change Password This allows the current user to change his her password which will then apply to any subsequent log ins Users This allows a user of Level 3 to add or delete users from the systems database Login This allows the user to login using his her User Name and Password The User Level will become that of the newly logged in user Notes on User Level Restrictions User Level 1 Intended for users who can operate the SOLO but are not allowed to open change save or close methods or use the Communication utility Level 1 users can run already loaded methods teach modify deck positions and change their own passwords User Level 2 Level 2 users cannot create new methods add or delete users or use the C
41. sage Please Press the GREEN Button when ready to proceed This option allows a suitably configured SOLO to pause here until a digital input signal with the value of Target Value 1 or 0 is received from another device such as a robot or a user operated pushbutton A Accessory 1of1 52 3 15 4 Write To Output Selecting the Write To Output action for this step will change this step s display to appear as follows C Fie Edit Action View Tools Window Help Disluj a e plimi uot 4 UX a Step 1 of 1 801 Send Output Signal Change plate types Write To Output Output 33 Target Value fi y Current Value lo A Accessory 1of1 This option allows a suitably configured SOLO to set a digital output signal to the Target Value 1 or 0 to provide a signal to device such as a robot or a mechanical system 3 15 5 Hot Cold Nest If the SOLO is equipped with the Hot Cold Nest accessory the user can select the temperature setpoint as well as several optional ways to use this temperature controlled nest ey et 44 TX A Step 1 of 1 90 11 Operate Hot Cold Nest Change plate types Operate Heating Cooling Nest 7 IZ Tum Nest OFF at End of Method Actual A Accessory Set Desired Temperature 37 9 1of1 Don t Wait C Wait Until Actual SetPoint C Wait Until Temperature is Stable Tum Nest OFF Now M Turn Nest OFF at Temperature Log Te
42. there will be different hardware setups before you start using your instrument We currently carry three types of SOLOs 1 Ivek Pump 125ul or 250ul 2 Hamilton Pump syringe size optional 3 MultiChannel 8 or 12 Channel configuration 2 1 Ivek Pump Setup The Ivek Pump SOLO comes ready to run from the factory The Mandrel is shipped on the unit so there is no need to setup anything on the unit See Figure 2 1 Figure 2 1 2 2 Hamilton Pump The Hamilton pump SOLO is disassembled during shipping to prevent damage The glass syringe from the pump is removed before shipping The mandrel for a disposable tip or the fixed cannula tip is also removed before shipping You may need to home the unit first before you can reinstall the Glass syringe The cannula tip mandrel is installed by pulling out the plunger that locks the mandrel into the Z axis assembly See Figure 2 2 Plunger for mandrel Figure 2 2 2 3 Multichannel The Multichannel SOLO is disassembled during shipping to prevent damage The Multichannel head is removed for shipping To set up the Multichannel head is pressed into the SOLO s z axis Align the Multichannel head assembly with the Pipettor Drive assembly locking stud See Figure 2 3 You will then press the Pipettor head firmly into place until you hear the locking mechanism snap in place See Figure 2 4 Lock Engaged Figure 2 3 Figure 2 4 The multichannel SOLO has two deck configu
43. ts is nested inside a loop with 2 repeats the steps inside the inner loop will be executed a total of 6 times while those within only the outer loop will be executed 2 times SOLOSoft will pair each Loop step with the very next End Loop that appears in the method Any Loop without a matching End Loop will be ignored Fi C HudsonSOLOWDocument 1 hso Enter the NUMBER of Loops to Perform 3 1 for Continuous Loop Aspirate 2 of 4 Dispense 3 of 4 EndLoop End 4 of 4 3 7 Pause Step ITI This step shown in the next figure allows the user to pause the run and present i 38 a message to the operator It also allows the user to permit the operator to end the method at the time of the pause 1f for instance no liquid was aspirated The user types the desired message into the Enter pause message box and allows the operator to end the method by checking the Allow user to end run at this step box D C HudsonSOLOWocument 1 hso Pause Options aletle Y XK A AL md Enter pause message Sen iu a Change Plate Types The SOLO is PAUSED Allow user to end run at this step F Automatically continue after seconds Aspirate 3 of 5 The user may also simply set a timer in seconds to pause the method here then automatically resume after the time has elapsed 3 8 Move Arm Step E This step shown in the next
44. u Operate Ten Way Valve Change plate types Ten Way Valve Set Valve Number f4 y A Accessory 1 of 1 This option allows a SOLOPlus with an attached Ten Way Valve input for the Micro10x to modify the fluid being dispensed by the Micro10x Execution of the step results in a change in the input valve port on the Ten Way Valve It is recommended to re prime the Micro10x after switching fluids Optionally the user may want to empty the Micro10x before changing fluid sources to conserve liquids 51 3 15 3 Shaker Nest Selecting the Shaker Nest for this step will change this step s display to appear as follows apnjsjsj TK a E BONI x Operate Shaker Nest Change plate types Shaker Settings Number of Shaker Nests on SOLO Deck 3 A Accesson Speed Duration Accel 1of1 Unit No RPM sec sec Pattern M 1 500 120 25 NWSEw Vv 2 300 360 15 NS X V 3 750 240 25 Ev M 4 100 1 25 NWSE This enables the user to set the speed duration acceleration and mode of shaking action for up to 4 nests in one step if it is so equipped 3 15 4 Wait For Input Selecting the Wait For Input action for this step will change this step s display to appear as follows 6 File Edit Action View Tools Window Help Disiaj S ejej m 2 IES YX al Step 1 of 1 SO w vilu Wait For Input Signal Change plate types Wait For Input ES Input 2 Target Value fi y Current Value lo Mes
45. with or without filters 10 20 50 100 250 uL Disposable tips with or without filters 10 20 50 100 250 uL or Stainless Steel Pipetting Cannula various gages and length are available Positioning Precision X axis 0 025mm Y axis 0 025mm Z axis 0 004mm Motion Range X axis 594mm 23 3 8 Y axis 244mm 9 5 8 Z axis 101mm 4 Liquid handling features Pipetting M v Bulk Dispensing N A v No of fluid sources N A 1 3 5 7 Syringe size 125 250uL 25 10 000 uL Wash Pump N A Delivery of wash solution by peristaltic pump Flow 7 14 21 28 mL Software adjustable Safety features Password protection Emergency Stop E stop Button located on the front of the dispenser chassis Dimensions W 556mm 30 x D 483mm 19 x H 610mm 24 Weight 27 kg 60 1b Power requirements Power 60VA Voltage 100 240VAC Frequency 50 60HZ Operating conditions Temperature 15 32 C 59 90 F Relative humidity 30 80 non condensing Regulatory compliance Computer requirements PC with Microsoft Windows XP or newer Quad Core 2 5 Ghz 3 Gigabytes free hard drive space RAM 4 Gigabytes 1 USB port 15 SOLO Multichannel Hardware features Arm configuration Multiple liquid handling arm Tip configuration 12 or 8 Channel Disposable tip Tip variety Disposable tips with or without filters 20 or 200 uL Positi
46. with the Liquid Level Sensing option allow the user to check the box Liquid Level Detect box which will cause the pipette tip to seek the surface of the liquid in the well or vessel being aspirated If this is checked a text box labeled Max Depth mm is shown The user should enter the maximum depth of the tip below the 33 upper rim of the well or vessel below which it will not proceed if no liquid is detected If aspirating from a defined microplate and the entered Max Depth value exceeds the Plate Depth value that plate s Plate Depth value will be used so as not to drive the tip beyond the bottom of the well A typical display showing the Liquid Level Detect feature is shown below C HudsonSOLOWOLOSaft C Dacuments and Settings Phil Farrelly My Dacumenis Hudson Robotics SOLOSoftiDocument 1 hso 5 Sle For atan view Tare whdnw Heh nsw el me liijm Step 1 of 1 MEN Htjeli 1X AI kopirate Bone x Synge fal Siathy Faire Surge Mica Mena Change plate types fiz gt Stat My volume 79 ul Syrnor Spare Pressprate Disgens gt leiaht 4 mn 100 v r ul fabcve DOTTIM srat 6 1 ict a 9 Y Asprate Shif amaunis are mees rec from the o ul TAUG IT POSITION Z Shfl is fror the ligad level MativeWwell Max Leptalnnj 0 Ente Ihe Ju cuid eval ietzct Do Tip Touza Select Tyo of Suave number ct wells Aspiicle Shll hmi Te uuch Sd fri f c be asprated Aspiate firer Plate asi
47. ws columns and are read in left to right top to bottom order As in the case of aspirating from a plate position above any zero or negative volume values will simply be skipped The SOLO will aspirate the next non zero volume in the file Ifinside a Loop it will use the value following the last one aspirated during the previous execution of this step A complete list of the values to be aspirated can be viewed in the dropdown list inside the Aspirate Volume uL box 32 C Program Files SOLOSoft SOLOSoft Document 1 e File dit Action View Tools Window Help plal S mal gt 2 htop TX a Step 1 of 1 Aspirate IF Find Bottom of Vessel or Well Hido Mix Cycles 3 amp ma li us alts Tip will rise Z Shift above Dottom iv Mix at si Start Mix Volume so ul Syringe ul m Start by Emptying Dispense Height zu Change plate types 125 tinge POINT Te mm e Aspirate Backlash 101 p ul Syringe Speed Pre Aspiate Delay After Dispense 1 0 sec a e ul 3 1o y Dwell after Aspirate 2 C sec amp spirat2 Shift amounts are measured from the location of the TAUGHT POSITION Ea Plate Z Shift iz from JOTTOM of WELL Aspirate Volume uL ja I Dn Tip Touch Selec Type of Source oo Aspirate Shift mn Tip Touch Shift mm C Aspirat gt trom Plate Position E Po ift o r l Aspirate Point oR o 5 0 Aspiral hun Nanzu Puirt Postini v Y Shit 0s Y Shitt Po 2 Shit 6 ZShitt
48. y E A Positive Z is Shift from Switch to File Data Upwards Aspirate Point Aspirate Shift This enables the user to adjust the actual aspiration location from the taught position selected for aspiration This enables the user to go to a non standard location or to move in other than well spacing increments For plates the Z Shift value is the distance above the well bottom that aspiration will occur with a maximum value of the plate s well depth For named points it is the distance above or below that taught point s height Do Tip Touch Tip Touch Shift This enables the user to do a tip touch to clean a droplet off the tip and to specify where that tip touch will be relative to the actual aspiration location Any shift done in the Aspirate Shift selection above will affect this aspirate location This will cause the tip to touch twice on opposite sides of the well or vessel Aspirate Volumes table This graphic shows an image of the plate type selected for this step and enables the user to enter distinct volumes for each well in the plate Important be sure to pres lt ENTER gt after entering the last volume into any well location as it will not be saved if you don t Asa convenience you may enter a value in the first element of any row or column then click on the row column number to set every well in that line to that value Liquid Level Detect Units equipped
49. y the distance in the Z Shift box under the Aspirate Shift parameters Mixing If the box is checked immediately after either dispence or aspiration the SOLO will dispense then aspirate the amount in Mix Volume for the number of times given by the Mix Cycles value ending with a final dispense to leave the mixed sample in the well or vessel The aspirating action of the mixing will take place at the height indicated by the Z Shift entry while the dispense action will occur at the Dispense Height distance from the well bottom for plates or from the taught point Valve Port D syringe type only not shown The user must select the appropriate fluid reservoir by choosing the correct valve from a drop down list for the selected syringe pump 30 Syringe Speed Scroll up or down to accelerate or slow the syringe piston s movement Pre Aspirate Enter this volume to aspirate air before each aspiration event in this step This allows air pockets to separate each individual aspirated sample and also allows the dispense volume to blowoff with an air pocket to forcibly expel tip droplets at the end Backlash Enter the number the default is 0 of uL that you wish to have the syringe piston move in reverse at the end of its stroke This is used to control droplets or end of tip air gaps also used in a Dispense step for a given commanded volume Multiple Wells The number of
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