User instructions Compound laboratory microscope KERN OBF, OBL
Contents
1. eese eee ieee ense eee enne nnn nnne nn nnn 12 6 SEO AC i NEED EE IE IE 14 7 Malntena tt i iii iew aa Edo Gee 14 7 1 Ghan ing the DUD socio ssc rn 14 7 2 Ghang in the FSC i i a 14 8 Trouble SHOOTING iii Eod Dn E be E gue enu e Use sR URL S LENS 15 9 SEVICE i cR 18 10 EUS OSA i G GG nn nnne 18 11 Further INTO MATION sassi 18 OBF OBL BA e 1510 1 Before use 1 1 General notes You must open the packaging carefully to make sure that none of the accessories in the packaging fall on the floor and get broken In general microscopes should always be handled carefully because they are sensitive precision instruments When using or transporting the Microscope it is particularly important to avoid abrupt movements as this may damage the optical components You should also avoid getting dirt or finger prints on the lens surface because in most cases this will reduce image clarity To maintain the performance of the microscope it must never be disassembled So components such as lenses and other optical elements should be left as they were before use Also the electrical parts in the base of the device must not be tampered with as in this area there is an additional risk of triggering an electric shock 1 2 Notes on the electrical system Before connecting to a mains power supply you must make sure that you are using the correct input voltage The information to select the correct mains2. 4 Eyepieces For each eye use an eyepiece with the same magnification Remove the plastic protective caps and insert both eyepieces They are not fixed with a screw but are just inserted Please make sure that you do not touch the lenses with your fingers and that no dust enters the apertures OBF OBL BA e 1510 10 5 Operation 5 1 Getting started Plug the mains plug into the socket and plug the cold device plug into the device Set the dimmer to the lowest level Switch on the main switch 5 2 Adjust the gap between the eyes Different users have different gaps between the eyes So each time a different person uses the microscope the gap between the two eyepieces must be re adjusted While you are looking through the eyepieces use one hand to hold the right or left lens barrel firmly By pulling them apart or pushing them together you can either increase or reduce the gap between the eyes As soon as the left and right visual fields fully overlap each other only one circular image is visible this is the correct gap between the eyes 5 3 Adjusting the magnification select the lens with the lowest magnification and make sure that the lens revolving unit is properly clicked into place Place the test object on the specimen stage and fix it with the object holder if necessary Use the control dial for the X Y axis specimen stage to position the sample in the correct place central under the middle of the lens Carefully
3. A 1354 OBB A 1349 OBB A 1350 OBB A 1352 OBB A1111 OBB A1108 OBB A1112 OBB A1109 OBB A1110 60x 0 80 spring OBB A1113 OBB A 1255 4x 0 10 10x 0 25 40x 0 65 spring Plan objectives OBB A 1238 OBB A 1256 OBB A 1239 100x 1 25 oil spring 60x 0 80 spring Siedentopf 30 inclined 360 rotatable Binocular tube Interpupillary distance 50 75 mm for non infinity system With diopter adjustment one sided OBB A 1249 OBB A 1269 OBB A1129 Siedentopf 30 inclined 360 rotatable Interpupillarv distance 50 75 mm Light distribution 20 80 for non infinity system With diopter adjustment one sided Stage size WxD 145x140 mm Travel 76x52 mm Coaxial coarse and fine focusing knobs scale 2 um Two slide holder OBB A 1345 Trinocular tube Mechanical stage Condenser Abbe N A 1 25 precentered aperture diaphragm OBB A1103 OBB A 1307 6V 20W Halogen transmitting Illumination 3W LED illumination system transmitting non rechargeable Field diaphragm Field diaphragm N A 0 9 Dry Darkfield unit Usable for 4x 40x objectives OBB A1149 OBB A 1277 100W HBO Epi Fluorescence unit three hole slide B G including centering objective Fluorescence unit 3W LED Epi Fluorescence unit three hole slide B G including centering objective 0 47x focus adjustable B FO ef 1x e Standard config
4. cable is located on each device on the rear of the product directly above the connection socket You must comply with this information If you do not comply with these specifications then fires or other damage to the device could occur The main switch must also be switched off before the mains cable is connected In this way you will avoid triggering an electric shock 3 OBF OBL BA e 1510 If you are using an extension cable then the mains cable you use must be earthed If the original fuse should blow it must only be replaced by an appropriate fuse ouitable replacement fuses are included with the delivery When carrying out any procedures whereby you come into contact with the electrical system of the device such as for example changing the bulb or fuse only carry out these procedures when the power is disconnected Under no circumstances should you touch the integrated halogen bulbs or housings either during operation or directly after use These bulbs produce significant heat and therefore there is a risk that the user could be severely burnt So before handling the bulbs you must check that they have cooled down 1 3 Storage You should ensure that the device is not exposed to direct sunlight temperatures which are too high or too low vibrations dust or a high level of humidity The ideal temperature range is between 0 and 40 C and a relative humidity of 8596 should not be exceeded The device should always be locate
5. the field diaphragm adjusting ring Open the field diaphragm until it just disappears out of the visual field For each slide use the aperture diaphragm of the condenser to find the very best compromise between contrast and brightness for the microscopic image The scale on the condenser is to be regarded as a recommendation for each lens It is possible to alter the brightness of the bulb using the dimmer The brightness is always controlled by the bulb brightness and not by the field diaphragm It is possible to re adjust the focus and x y axis Observe the object 5 5 Dioptre compensation and focussing For models with dioptre setting Keep the eye on the fixed non adjustable lens barrel open and close the eye which is on the adjustable lens barrel Then adjust the focus for the open eye using the fine adjustment knob Then open the other eye and shut the eye which had been open Then adjust the focus for the second eye on the dioptre setting When doing this do not touch the fine adjustment knob If necessary alter the brake action for the control knobs using the locking ring The rotary Knobs on the lefthand side are connected to the rotary knobs on the righthand side Please only rotate on one side at a time do not rotate right and left at the same time in opposite directions otherwise this will damage the drive Using eye cups If eyepieces with a high eye point particularly suitable for those who wear glasses are used th
6. Disposal The packaging is made of environmentally friendly materials which you can dispose of at your local recycling centre Disposal of the storage box and device must be carried out by the operator in accordance with all national or regional laws in force in the location of use 11 Further information The illustrations may differ slightly from the product The descriptions and illustrations in this user manual are subject to change without notice Further developments on the device may lead to these changes Al language versions contain a non binding translation The original German document is the binding version 17 OBF OBL BA e 1510 NOTES OBF OBL BA e 1510 18
7. K E Fe N l Aeria wn 9933 0 D 72336 Balingen Fax 49 0 7433 9933 149 f e OPTICS E mail info kern sohn com Internet www kern sohn com User instructions Compound laboratory microscope KERN OBF OBL OBF121 OBF122 OBF123 OBL125 OBL127 Version 1 0 01 2015 OBF OBL BA e 1510 KERN OBF OBL Version 1 0 01 2015 User instructions Compound microscopes Table of contents 1 Belore USE pasa 3 1 1 General Not Sisic aE a EAE DAEAR A RARE 3 1 2 Notes on the electrical system uiid iste d ad la 3 1 3 SFO il GE e M te 4 1 4 Maintenance and eleanifig ENE le sladrede ees 5 2 Nomernclatule mese ika a dave cure dud e dataset nu CU dune CUNG ot tUU duds ER 6 3 Technical data model outfit 8 4 trie 10 4 1 EI 10 4 2 Condenset ee ln 10 4 3 api 10 4 4 pls nte 10 5 OPC Trig ee 11 5 1 Gettin State d i eo ERE eo PUn EE repete Enero ccrte adr ev ec Eon Veo ud uu EER Sce EES NE 11 5 2 Adjust the gap between the eyes eeliieeeeee ee ieeeeeene eese nennen nnn nnne n anna anna n nnns 11 5 3 Adjustin the magnifications siccus duse ouo su pax Lc cd teens coUa ud a Deu Urdu ce aa a a uM e Dumas cer Uno dels 11 5 4 Adjust ie DrIgh liess ioni cuta ise med a cues n Cela sa caue veu clon a a GRE CuCudu secu uiv ceu Dra Roda 12 5 5 Dioptre compensation and FOCUSSING
8. d on a rigid smooth horizontal surface When the microscope is not being used you should fit the lens cap and cover the microscope with the enclosed dust protective cover If the eyepieces are being stored separately the protective caps must be fitted to the lens barrels In most cases if dust and dirt gets inside the optical unit of a microscope this can cause irreversible errors or damage The best way to store accessories which consist of optical elements such as for example eyepieces and lenses is in a dry box with desiccant OBF OBL BA e 1510 1 4 Maintenance and cleaning In any event the device must be kept clean and dusted regularly If any moisture should be occur before you wipe down the device you must ensure that the mains power is switched off When glass components become dirty the best way to clean them is to wipe them gently with a gauze cloth To wipe oil stains or finger prints off the lens surface moisten the gauze cloth with a mixture of ether and alcohol 70 30 ratio and use this to clean the lens You must be careful when handling ether and alcohol as these are highly flammable substances You must therefore keep it away from naked flames and electrical devices which can be switched on and off and only use it in well ventilated rooms However organic solutions of this type should not be used to clean other components of the device This could lead to damage to the paint finish To do this it is suff
9. en it may also be useful for users who don t wear glasses to fit eye cups to the eyepieces These special eyepieces are also called High Eye Point eyepieces They can be identified by the glasses symbol on the side OBF OBL BA e 1510 12 Eye cups We 16x113 6 High Eye Point eyepieces identified by the glasses symbol When doing this make sure that the dioptre setting is not moved We would therefore advise that you hold the dioptre compensation ring on an eyepiece with one hand while you fit the eye cup with the other Before using the microscope users who wear glasses must remove the eye cups which you may find on High Eye Point eyepieces Make sure that you maintain the hygienic cleanliness of the eye cups Turning the lens revolving unit will bring the lens with the next magnification level into position Make sure that the lens clicks properly into place When rotating the lenses check from the side to make sure that the glass holder does not get damaged by the lens Under normal conditions you should be able to select the next lens immediately and the focus should be roughly at the same level Make additional adjustments with the fine adjustment knob If when you rotate the lens it hits the object holder you must first lower the specimen stage and re adjust it For lenses which work with oil immersion these are marked with the word oil first put the oil drops on the slide bring the lens into position and move it closer
10. icient to use a neutral cleaning product You could also use the following cleaning products to clean the optical components e Special cleaner for optical lenses e Special optical cleaning cloths e Bellows e Brush When handled correctly and checked regularly the microscope should give many years of efficient service Should repairs still be necessary please contact your KERN dealer or our Technical Department 5 OBF OBL BA e 1510 2 Nomenclature Lens barrel connectors Eyepiece Head Adjusting screw Head Lens revolving uni Lens Object holder Control dial X Y axis opecimen stage opecimen stage Condenser Coarse Aperture adjustment adjustment Condenser knob Fine Field lens with adjustment Field diaphragm knob Dimmer OBF OBL BA e 1510 6 Rear view Carrying handle d Adjusting ring A torque Fine p a adjustment knob Main switch 8 Coarse A adjustment knob Mains connection Adjusting ring torque Condenser Focus dial 7 OBF OBL BA e 1510 3 Technical data model outfit Standard configuration Optical system Tube Illumination inocular 6V 20W Halogen transmitting OBF121 B LOBF 122 Achromatic Binocular 6V 20W Halogen transmitting OBF 123 Achromatic Binocular 3W LED transmitting OBL 125 6V 20W Halogen transmitting ity inocular 3W LED transmitting Order number Model outfit Model KERN OBB A 1347 OBB
11. ith PH Plan objective 100x o o0 OBBAIZI3 OBB A1213 A1213 100W HBO Epi Fluorescence unit three hole slide B G Fluorescence unit 3W LED Epi Fluorescence unit three hole slide B G 0 47x focus adjustable 9 OBB A1135 C Mount e Standard configuration Option 9 OBF_OBL BA e 1510 4 Assembly 4 1 Lenses The specimen stage must be in its lowest position so that the lenses can be screwed into the lens revolving unit The lenses are screwed in so that when you turn the revolving unit the next lens for stronger magnification moves into position Please make sure that you do not touch the lenses with your fingers and that no dust enters the apertures For lenses which are marked OIL you must use an immersion oil with the lowest level of inherent fluorescence 4 2 Condenser Move the specimen stage to the upper position Use the focus dial of the condenser to move the condenser holder to the central position Fit the condenser into the condenser holder from below and fix with the adjusting screw When doing this you should be able to read the scale from the front Please do not touch the optical lenses with your fingers 4 3 Head Undo fixing screw Insert the round dovetail bracket on the head into the round dovetail bracket in the housing and tighten with the fixing screw Please make sure that you do not touch the lenses with your fingers and that no dust enters the apertures 4
12. plug is not correctly plugged The bulb does not light There is no power at the socket Defective bulb Defective fuse The specified bulb or fuse has not been used The aperture diaphragm and or field diaphragm are not opened wide enough The selector switch for the beam path is set to Camera The brightness control has been set incorrectly The condenser has not been correctly centred The bulb blows immediately The visual field is dark You cannot adjust the brightness The condenser is too low The visual field is dark or is not correctly The lens has not been correctly adjusted illuminated The selector switch for the beam path is between two settings The object revolving unit is not correctly fitted The condenser is not correctly fitted A lens is being used which doesnt match the lighting area of the condenser The field diaphragm is closed too tightly The bulb is not correctly fitted The visual field of one eye does not The gap between the eyes is not match that of the other eye correctly adjusted Dioptre setting has not been carried out correctly Different eyepieces are used for the right and left side The eyes are not used to using a microscope 15 OBF OBL BA e 1510 The aperture diaphragm is not Blurred details opened wide enough Bad image The condenser is too low The lens does not belong to this microscope The front lens of the lens unit is dirty An immer
13. raise the specimen stage using the coarse adjustment knob until the slide is almost touching the lens observe from the side The lens must not touch the object holder risk of glass breakage Look through the eyepieces and use the coarse adjustment knob to lower it slowly until the object is visible If necessary adjust the brightness by adjusting the bulb potentiometer Avoid overexposure Now use the fine adjustment knob to adjust the focus For slides with low contrast or a small number of objects for example individual cells we recommend that you use a high contrast standard slide to help you to make the adjustment to find the correct level Once you have found a point which must be observed more closely this must be adjusted to be in the middle of the visual field x y adjustment of the stage To be able to view the required section of the object this section must be positioned centrally under the lens 11 OBF_OBL BA e 1510 5 4 Adjust the brightness Adjust the condenser aperture Close the field diaphragm in the base either completely or almost completely When you look in the microscope a blurred image appears in the aperture Adjust the height of the condenser until the image from the field diaphragm appears clearly in the visual field For some microscopes there is a risk that you will lift the condenser up so high that it collides with the object holder Care is needed when doing this Adjust the field diaphragm using
14. sion object has been used without immersion oil Bad contrast Vignetted visual field The immersion oil contains air bubbles The recommended immersion oil has not been used Dirt dust on the lens Dirt dust on the front lens of the condenser Dirt or dust in the visual field Dirt dust on the eyepieces Dirt dust on the front lens of the condenser Dirt dust on the object The stage has not been assembled correctly The lens is not positioned correctly on the beam path The lens revolving unit is not correctly fitted The upper side of the object is facing down The lens revolving unit is not correctly fitted The lens is not positioned correctly on the beam path The coarse adjustment knob is difficultto The rotational resistance brake is turn overtightened The angle table is blocked by a solid body The rotational resistance brake is not tight enough One side of the image is blurred The image flickers The stage moves down on its own The fine adjustment knob moves on its own When you move the table the image The stage was not correctly fitted becomes blurred OBF_OBL BA e 1510 16 9 Service If after studying the user manual you still have questions about commissioning or using the microscope or if unforeseen problems should arise please get in touch with your dealer The device may only be opened by trained service engineers who have been authorised by KERN 10
15. to the object holder The link between the object holder and lens is the oil After use or before changing the slide remove the oil using a dust free cloth 13 OBF OBL BA e 1510 6 Storage It is important that the microscope is covered with the enclosed protective cover after each use In the long term this is the only way to protect it from getting dirty Ideally the microscope will be used in an air conditioned room If the microscope is not used for several days then we would recommend that it is stored in a cupboard with its protective cover Dust and moisture are the greatest enemies of the microscope 7 Maintenance In general these microscopes are maintenance free Service procedures should be limited to replacing bulbs light sources and electrical fuses Should a defect occur please contact your dealer or KERN Service 7 1 Changing the bulb owitch the device off and unplug it Unscrew the cover on the rear of the device and open it up Wait until the bulb has cooled down halogen bulb Remove old bulb Please use cloth gloves to hold and fit the new bulb otherwise grease and dust on the surface of the bulb could affect its brightness and service life 7 2 Changing the fuse owitch the device off and unplug it Pull out the fuse housing under the mains cable socket remove the old fuse and fit a new fuse Push the fuse housing back in OBF OBL BA e 1510 14 8 Trouble shooting Problem Possible causes The mains
16. uration Q Option OBB A1154 OBB A1157 OBB A1178 OBB A1194 OBB A1203 OBB A1135 OBB A1142 Co OBF OBL BA e 1510 Model outfit Model KERN 00 0 rm wie i s ema WF 1ox 970 mm er mi shat e 95A wow Dem id meme e s mo Psom e 9 one Siedentopf 30 inclined 360 rotatable Binocular tube Interpupillary distance 50 75 mm for infinity system OBB A1130 With diopter adjustment one sided Siedentopf 30 inclined 360 rotatable i Interpupillary distance 50 75 mm Jonooular tube Light distribution 20 80 for infinity system SIDES IIS With diopter adjustment one sided T D Stage size WxD 145x140 mm Travel 76x52 mm Mechanical stage Coaxial coarse and fine focusing knobs scale 2 um Two slide holder Abbe N A 1 25 precentered aperture diaphragm OBB A1103 A1103 6V 20W Halogen transmitting E OBB A 1370 3W LED illumination system transmitting non rechargeable Field Field diaphragm Field diaphragm NA 0 9 Ory Polarisingunit unit Analyser Polariser o 9o oOBAZ OBB A1277 A1277 Independent slot with PH Plan objective 10x 9 amp OBB A1215 Independent phase contrast unit Independent slot with co PH Plan objective 20x amp OBB A1217 including PH condenser and Independent slot with PH Plan objective 40x aa S OBB A 1219 PH slides Independent slot w
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