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IEF, SDS-PAGE & 2–D Electrophoresis

1. NovaBlot Kit components A NovaBlot electrode cathode B NovaBlot electrode anode C Electrode paper NovaBlot 200 x 250 mm D Cellophane sheets 210 x 320 mm Product Quantity Code Number General Accessories Film remover 1 18 1013 75 FlexiClamps 6 18 1013 73 Roller 1 80 1106 79 Cooling Plates and Accessories Cooling plate ceramic 210 x 270 mm 1 18 1103 46 Insulation for cooling tubing 14 mm i d 27 mm o d 1 8m 80 1116 11 Glass Plates and Trays Glass plate 84 x 94 x 1 mm 50 80 1106 69 Glass plate 260 x 125 x 3 mm 2 80 1106 99 Glass plate 260 x 125 x 1 mm 15 80 1106 29 Glass plate 200 x 260 x 4 mm 2 18 1102 47 Glass plate 125 x 260 x 0 5 mm U frame 2 80 1106 89 Glass plate 125 x 260 x 1 0 mm U frame 2 80 1106 91 Glass plate 125 x 260 x 2 0 mm U frame 2 80 1106 92 Glass plate 125 x 260 x 0 5 mm U frame Reswelling 1 80 1106 95 cassette Glass plate 125 x 240 mm 0 5 mm spacer 3 80 1106 94 Glass plate 200 x 260 x 0 5 mm U frame 2 80 1106 87 Glass plate 200 x 260 x 1 0 mm U frame 2 80 1106 88 Glass plate 125 x 270 x 6 mm UltroMold 1 80 1115 81 Glass plate 125 x 490 x 6 mm UltroMold 1 80 1115 80 For pricing information visit www gelifesciences com orderonline GE Healthcare SDS PAGE IEF Standard Flatbed Units ORDERING INFORMATION Product Quantity Code Number NovaBlot Kit 1 18 1016 86 Includes NovaBlot electrode cathode 1 18 1019 86 NovaBlot electrode ano
2. Quantity Code Number 1 80 6442 90 For pricing information visit www gelifesciences com orderonline Multiphor II Buffer Strip Positioner is designed only for use with the current Multiphor II cooling plate see Multiphor II Electrophoresis Unit see page 291 image D It cannot be used with earlier models of the cooling plate For more information visit www gelifesciences com udo1125 3 O me OD T 295 p T QO a O ma O O ad jad Q O LLJ 296 SDS PAGE IEF Standard Flatbed Units CleanGel for IEF e Perform sensitive procedures using precast gels that are carefully washed and dried after casting to remove catalysts and unpolymerized material e Customize IEF separations by reswelling in a suitable mixture of carrier ampholytes and other additives such as detergents or denaturants e Handle mechanically stable plastic backed gels with confidence CleanGel IEF precast gels are dried empty gels ready for reswelling in q carrier ampholyte solution of choice CleanGel IEF gels offer improved gel performance with faster and more stable gradients fewer artifacts and easier zymogram assays Before use CleanGel IEF gels are rehydrated using the convenient GelPool accessory Swelling returns the gel to its original thickness of 0 5 mm Immobiline DryPlate for IEF e Obtain reproducible IEF separations with dry precast gels containing immobilized pH gradients e Choos
3. see page 291 Multiphor Il Electrophoresis System miniVE Vertical SE 600 Ruby Electrophoresis System see page 291 see page 287 see page 283 284 24cm Electrophoresis Resolution throughput and loading capacity 09 CO A A A 3 3 3 Choices for second dimension SDS PAGE 7 cm Blotting see page 347 Automated gel staining and blot p rocessing see page 370 Gel and blot imaging and analysis see chapter 10 260 For more information visit www gelifesciences com GE Healthcare 2 D Electrophoresis The choice of q sustem for performing second dimension SDS PAGE depends on several factors These include e Length of IPG strip used in the first dimension seporqtion e Throughput with regards to the number of gels per run and the number of 2 D maps per gel e Separation time These factors are summarized for each system in the selection guide below SELECTION GUIDE Second Dimension SDS PAGE Systems Do you want to run SDS gels horizontally or vertically Horizontally m with any size Vertically D IPG strip O O What is the size of your IPG strip O OD 17 24 cm a N How many SDS gels do you What is your maximum SDS gel size need to run simultaneously 10 x 10 5 cm 1 gel 1 2 gels 1 2 gels 1 2 gels 1 4 gels 1 6 gels 1 12 gels Multiphor II any size miniVE SE 250 SE 260 strip Ettan Ettan DALTsix DALTtwelvet To use four gels requires access
4. Full Range Rainbow Molecular Weight Markers IMPROVED 250ul RPN800E High Range Rainbow Molecular Weight Markers 250 ul RPN756E IMPROVED Low Range Rainbow Molecular Weight Markers 250 ul RPN755E IMPROVED For pricing information visit www gelifesciences com orderonline Store at 15 C to 30 C Related Products Refer To Amersham ECL Plex Fluorescent Rainbow Markers page 355 Amersham ECL DualVue Western Blotting Markers page 317 miniVE Vertical Electrophoresis System page 283 SE 600 Ruby and SE 660 Dual Cooled Vertical Units page 287 ImageQuant Imagers page 384 Full Range High Range Low Range RPN8OOE RN756E RNP755E M x 103 Color M x 103 Color M x 103 Color 225 Blue 225 Blue 38 Blue 150 fm Red 31 Orange 24 Green 102 E Green 76 Yellow 76 WN yellow i Blue 52 Purple 12 Red 8 5 Yellow Purple 38 Blue 38 Blue 31 Orange 3 5 Blue 24 E Green 31 SE Orange 24 WR Green 17 Blue 12 Red 17 Blue 12 m Red Rainbow Molecular Weight Markers are available in three size ranges for use with SDS PAGE GE Healthcare IEF SDS PAGE amp 2 D Electrophoresis Protein Molecular Weight Markers and pl Markers Amersham ECL DualVue Western Blotting Markers e Detect proteins on gel and membrane as well as on film ORDERING INFORMATION e Prestained markers indicate efficiency of protein transfer to Product Quantity Code Number membrane and blot orientation ECL DualVue Western Blot
5. 12 min Noise level at 1 m in front of the unit lt 63 dBIA Line voltage 95 132 VAC 60 Hz 95 125 VAC 50 Hz 198 253 VAC 50 Hz Power consumption 1200 W Maximum operating ambient humidity 80 at 31 C 50 at 40 C Operating temperature range 4 C to 40 C Maximum operating altitude 2000 m Dimensions W x D x H 22 x 42 x 48 cm Weight 28 kg Safety certifications For 230 VAC CE 89 336 EEC EMC directive CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 UL3101 1 CSA22 2 1010 1 Specifications are for 20 C ambient temperature See trademark information at back of catalog For more information visit www gelifesciences com GE Healthcare 2 D Electrophoresis Second Dimension Ettan DALTtwelve Large Vertical System DIG E approved Product Quantity Code Number Ettan DALTtwelve Separation Unit and Power 1 80 6466 46 Supply Control Unit 115 V With buffer circulation pump and Peltier cooling for up to twelve 26 x 20 cm gels Ettan DALTtwelve Separation Unit and Power 1 80 6466 27 Supply Control Unit 230 V With buffer circulation pump and Peltier cooling for up to twelve 26 x 20 cm gels DALT Gel 12 5 26 x 20 cm 6 17 6002 36 DALT Buffer Kit for 12 precast gels 1 17 6002 50 DALT Precast Gel Cassette 1 80 6466 65 DALT Gel Casting Cassette 1 0 mm 1 80 6466 84 DALT Gel Casting Cassette 1 5 mm 1 80 6488 69 Low fluorescence Glass Plates 27 x 21 cm for Ettan DALT 1 80 6475 58 m includi
6. DIGE approved ORDERING INFORMATION Product Quantity Code Number PlusOne Glycine 500g 17 1323 01 For pricing information visit www gelifesciences com orderonline Store at room temperature TECHNICAL SPECIFICATIONS M 75 07 Minimum assay 99 7 DIGE approved ORDERING INFORMATION Product Quantity Code Number Immobiline DryStrip Cover Fluid 11 17 1335 01 For pricing information visit www gelifesciences com orderonline DIGE approved Quantity Code Number 25g RPN6302 Store at room temperature ORDERING INFORMATION Product lodoacetamide For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product Quantity Code Number PlusOne Mercaptoethanol 25ml 17 1317 01 For pricing information visit www gelifesciences com orderonline Store dry at room temperature ORDERING INFORMATION Product Quantity Code Number PlusOne N N Methylene bisacrylamide 25g 17 1304 01 PlusOne N N Methylene bisacrylamide 100g 17 1304 02 PlusOne N N Methylene bisacrylamide 2 Solution 11 17 1306 01 For pricing information visit www gelifesciences com orderonline For more information visit www gelifesciences com udo1125 3 O x D T 311 n QO O S O O Q LLJ 312 Electrophoresis Standards and Reagents PlusOne Repel Silane ES Repel Silane ES is a 2 solution of dimethyldichlorosilane
7. Dept of Clinical Veterinary Science University of Bristol UK 10 Koide T and Ikenaka T Eur J Biochem 32 401 407 1973 Unpublished data 11 Brew K et al J Biol Chem 242 3747 3749 1967 2 Nakamura S et al Hoppe Seylor s Z Physiol Chem 356 677 692 1975 12 Spiro M J J Biol Chem 248 4446 4460 1973 3 Fowler A V and Zabin Proc Natl Acad Sci USA 74 1507 1510 1977 13 Bryce C F A and Crichton R R J Biol Chem 246 4198 4204 1972 4 Roberts R C et al J Biol Chem 241 4907 4913 1966 14 Takeda A et al J Biochem 78 911 924 1975 5 Eisenberg H and Tomkins G M J Mol Biol 31 37 49 1968 15 Huston S J et al Biochem 11 1609 1612 1972 6 Titani K et al Proc Natl Acad Sci USA 74 4762 4766 1977 16 Canfield R E J Biol Chem 238 2698 2707 7 Hirayama K et al Biochem Biophys Res Comm 173 639 646 1990 17 Molecular weight based on peptide sequence 8 Yao J et al Anal Chem 67 3638 3642 1995 18 Trautschold et al Biochem Pharmacol 16 59 72 1966 9 Reynaud J et al Biochimie 53 1095 1098 1971 GE Healthcare For more information visit www gelifesciences com eis n QO O O i jad Q O LLJ 316 IEF SDS PAGE amp 2 D Electrophoresis Protein Molecular Weight Markers and pl Markers Amersham ECL Plex Fluorescent Rainbow Markers For main product entry see page 355 Rainbow
8. EN 61010 1 IEC1010 1 UL3101 1 CSA22 2 1010 1 PhastTransfer Transfer technique Semi dry electrophoretic blotting Capacity 1 or 2 gels Transfer time 10 30 min Buffer required 5 ml gel PBTP thermoplastic polyester containing glass fiber Graphite Aqueous solvents and diluted alcohols in the pH range 2 11 20 x 15 x 3 cm 17x9x6cm For more information visit www gelifesciences com GE Healthcare SDS PAGE IEF PhastGel e Select from homogeneous and gradient polyacrylamide gels e Separate proteins and peptides on denaturing native and isoelectric focusing gels e Save time and improve reproducibility with precast gels buffer strips and staining kits e Achieve detection sensitivity comparable to that of radioisotopes with ready to use silver staining kits PhastGel media are polyacrylamide gels 43 x 50 x 0 45 mm W x Lx T ona polyester film backing With a wide variety of gel formulations available the small format has proven flexibility documented by dozens of application notes and hundreds of published references A dedicated PhastGel Kit is available for detection of oligoclonal IgG in unconcentrated cerebrospinal fluid CSF Analysis Kit includes gels and reagents for 40 analyses PhastGel Blue R PhastGel Blue R tablets are a readily soluble pre measured means of preparing Coomassie Blue R 350 solutions PhastGel Blue R 350 is more sensitive than the commercially available R 150 and R
9. Glass plate club sandwich divider notched 1 80 6179 18 O 18 x 24 cm x Glass plates 18 x 24 cm 2 80 6190 01 OD Glass plates 18 x 24 cm low fluorescence 2 80 6442 33 N Glass plate club sandwich divider notched 1 80 6190 20 T For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To EPS 601 Power Supplu 18 1130 02 poge 304 Rainbow Molecular Weight Markers IMPROVED page 316 SE 100 Plate Washer and Storage Unit 80 6116 29 page 290 Electrophoresis Reagents page 306 MultiTemp III Thermostatic Circulator page 276 ImageQuant Imagers page 384 Divider plates double gel capacity Run up to four gels up to 112 samples in a single run Divider plates convert single gel sandwiches into two gel club sandwiches which seal and fit into the casting stand exactly like single gels Run up to 24 cm long gels with SE 660 If gels longer than 16 cm are required GE Healthcare offers the SE 660 which runs gels up to 24 cm Except for gel length the SE 660 has identical features to those of SE 600 Ruby Immobiline DryStrip 7 cm gels Request Application Note 80 6445 94 for details For more information visit www gelifesciences com 287 SDS PAGE IEF Standard Format Vertical Units SE 600 Ruby SE 600 and SE 660 Components and Accessories Product Quantity Code Number SE 600 Ruby SE 600 and SE 660 Replacement Parts and Accessories Wonder Wedge plate separa
10. rapid screening of protein and nucleic acid samples Gels are efficiently cooled using the heat conductive alumina gel plate and built in heat exchanger with or without connection to an external circulator The alumina back plate of each gel sandwich is in contact with buffer in one of the two buffer chambers which act as heat sinks The alumina plate conducts heat away from the gel sandwich into the upper buffer at a rate 40 times faster than glass of comparable thickness In addition a serpentine channel through which coolant can be pumped is permanently sealed between the two upper buffer chambers GE Healthcare Mini Format Vertical Units ORDERING INFORMATION Product Quantity Code Number SE 250 Mini Vertical Unit for two slab gels complete 1 80 6147 45 Includes Basic unit 10 glass plates two alumina notched plates well locating decal SE 245 Dual Gel Caster two each 0 75 mm thick 10 well combs and 0 75 mm thick spacer set SE 260 Mini Vertical Unit for two slab gels complete 1 80 6149 35 Includes Basic unit 10 glass plates two alumina notched plates well locating decal SE 245 Dual Gel Caster two each 0 75 mm thick 10 well combs and 0 75 mm thick spacer set For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To EPS 301 Power Supply 18 1130 01 page 304 Rainbow Molecular Weight Markers IMPROVED page 316 SE 100 Plate Washer and Storage Unit 80 6116 29
11. 250 forms and can detect most proteins at 50 100 ng band One tablet makes 400 ml of a 0 1 staining solution Silver staining kit protein For PlusOne Silver Staining Kit Protein 17 1150 01 see page 368 This product can be used for rapid sensitive reproducible staining of proteins in polyacrylamide gels including PhastGel media using the PhastSystem Development Unit Note the PlusOne Silver Staining Kit Protein replaces the original PhastGel Protein Silver Staining Kit 17 0617 01 PhastGel DNA Silver Staining Kit PhastGel DNA Silver Staining Kit supplies the reagents required to detect as little as 20 pg DNA per band using PhastSystem Development Unit After only 30 minutes developing time the stained gel can be dried for photography or storage Dried silver stained DNA gels also constitute an archive from which bands can be cut out and amplified Reference Heukeshoven J and Dernick R Electrophoresis 6 103 1985 GE Healthcare Automated Flatbed Units ORDERING INFORMATION Product Quantity Code Number PhastGel Buffer Strips DNA 10 pairs 17 1599 01 PhastGel Buffer Strips SDS 10 pairs 17 0516 01 PhastGel Buffer Strips Native 10 pairs 17 0517 01 PhastGel Gradient 4 15 10 17 0678 01 PhastGel Gradient 10 15 10 17 0540 01 PhastGel Gradient 8 25 10 17 0542 01 PhastGel Homogeneous 7 5 10 17 0622 01 PhastGel Homogeneous 12 5 10 17 0623 01 PhastGel Homogeneous 20 10 17 0624 01 PhastGe
12. 368 gel module blot module lower buffer chamber safety lid Amersham Blotting Labeling amp Detection Chapter 9 e Run two mini gels or four mini blots at once using 10 x ECT As epee SE 250 and SE 260 Accessories page 286 10 5 cm gel cassettes for increased separation distances e Complete semi wet blotting in 45 min with only 300 ml buffer e Choose precast gels from a variety of manufacturers e Perform two second dimension separations using 7 cm IPG strips in 1 5 h Amersham ECL Plex Western Blotting Combination Packs page 354 SE 100 Plate Washer and Storage Unit page 290 GE Healthcare For more information visit www gelifesciences com 283 n Q O S O O Q LL 284 SDS PAGE IEF Mini Format Vertical Units miniVE Vertical Electrophoresis System continued Convenient Design The simple modular design of miniVE Vertical Electrophoresis System consolidates all the small parts for electrophoresis and blotting into one convenient unit Uniform gel temperature is maintained with the tank style lower buffer chamber A full tank stabilizes the temperature so that lanes are straight and bands are flat The Blot Module will accept two gel membrane stacks simultaneously The lower buffer chamber is designed to hold two Blot Modules allowing four gels to be blotted at once Casting gels for miniVE System is as easy as load clamp Extra Length and Compatible Dimensions The miniVE
13. 40 3 296 Es pH 5 0 6 0 strip 26 52 1 40 3 296 a pH 5 6 6 6 strip 26 52 1 40 3 296 a Immobiline DryStrip 2 D First Dimension IEF 269 m IPG Buffer 269 m ExcelGel SDS 2 D Second Dimension 297 Buffers A Tris acetic acid pH 6 4 C Tris acetic acid pH 5 6 D Tris tricine pH 7 1 f Product to be discontinued by year end 2008 For an alternative to Ampholine PAGplate IEF please contact your local sales office 294 For more information visit www gelifesciences com GE Healthcare SDS PAGE IEF ExcelGel for SDS PAGE Sample application is quick and accurate with preformed sample wells e Save time with ready to use mechanically stable gels cast on plastic supports e Apply samples quickly and easily using preformed sample wells e Avoid making running buffer by using precast buffer strips TECHNICAL SPECIFICATIONS Standard Flatbed Units ORDERING INFORMATION Product Quantity Code Number ExcelGel SDS Gradient 8 18 6 80 1255 53 ExcelGel SDS Homogeneous 7 5 6 80 1260 01 ExcelGel SDS Homogeneous 12 5 6 80 1261 01 ExcelGel SDS Homogeneous 15 6 80 1262 01 ExcelGel SDS Buffer Strips anode and cathode Accessories Multiphor II Buffer Strip Positioner complete 1 80 6442 90 6 pairs 17 1342 01 For pricing information visit www gelifesciences com orderonline ExcelGel precast gels for SDS PAGE are available as homogeneous or gradient gels The homogeneous gels have 25 preformed sample wells
14. Caster 1 or 2 gels for up to 4 gels 18 x 16 cm plates Gel Caster 4 gels separately for up to 10 gels 18 x 16 cm plates Multiple Gel Caster 10 gels Includes 20 glass plates space saver plate combs and spacers separately 1h 1 1 80 6175 00 Includes 2 blank gaskets One included with each SE 600 Ruby unit 80 6191 34 Includes 8 glass plates 3 space saver plates 5 filler sheets 100 sheets of wax paper Spacer Mate alignment template and filler plugs Order combs and spacers 80 6182 79 5 filler sheets 100 sheets of wax paper and Spacer Mate alignment template Order Casting Accessories and Replacement Parts Short red clamps for gel casters Spacer Mate template for aligning spacers Polycarbonate filler sheets 18 x 16 cm Wax paper precut sheets 18 x 16 cm Acrylic space saver block 1 2 cm thick 18 x 16 cm 4 3 1 5 100 80 6148 02 80 6181 65 80 6181 84 80 6182 41 80 6182 60 For pricing information visit www gelifesciences com orderonline For more information visit www gelifesciences com udo1125 3 O me OD T 289 SDS PAGE IEF Related Vertical Equipment SG Gradient Makers t l gt k 3 3 l W j x SG Gradient Makers are durable and accurate e Form gradients for acrylamide gel electrophoresis liquid ORDERING INFORMATION Product Quantity Code Number SG 15 Gradient maker 15 ml total volume 1
15. DIBJONN e sju Bp y UOSN d e sion 4 3910 940 Q e S NI 13qv NOLWLILNVNO nAOUuDN e oid o01 32 dso4 n e ZT 191dpu2 s 1a aW0 0UdO1 DadS NOILYLILNYNO NOILWdVdsdd JIdWVS uonosodaid S CAWUDS PIDD D3I O9nN e UO O1Ndaid duups u 1Oid e S 1 1dpu2 s uol pipd id duips NOIWadVdsdd JIdWVS sis 1oudo115 3 JGIND NO 12313S GE Healthcare For more information visit www gelifesciences com 258 2 D Electrophoresis Introduction to Electrophoresis Tools for Proteome Studies e Identify proteins faster using q comprehensive range of overlapping Immobiline DruStrip gels that cover narrow 1 pH unit medium 3 5 pH units and wide 8 pH units pH ranges e Choose from two instrument platforms for first dimension isoelectric focusing IEF e Choose from five instrument platforms for second dimension SDS PAGE to meet any format resolution or throughput requirement e Choose Ettan DALTtwelve or Ettan DALTsix for use with 24 cm IPG strips and run up to 12 large format precast gels at once e Tap into extensive 2 D electrophoresis knowledge and technical support through seminars workshops application notes posters a 2 D handbook a video a dedicated Web site and other support materials Immobiline DryStrip gels for reproducible first dimension IEF with high resolution and loading capacity Immobiline DryStrip precast gels with matching IPG buffers simplify pe
16. DeCyder 2 D Differential Analysis Software DeCyder 2 D v6 5 has been specifically developed for 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE This software significantly increases throughput by accurately addressing measurement of protein differences with statistical confidence and reduces hands on time from days to minutes with minimal user to user variation The software automatically detects matches and analyzes protein spots in multiplexed fluorescent images and is able to detect small differences between protein spots with high statistical confidence DeCyder 2 D v6 5 consists of several modules e Differential In gel Analysis DIA co detection background subtraction normalization and quantitation of spots in images from a single 2 D DIGE gel e Biological Variation Analysis BVA matching multiple 2 D DIGE gels for comparison and statistical analysis of protein abundance changes e Extended Data Analysis EDA providing advanced statistical analysis for characterization and classification of biological samples based on protein expression data e Batch Processor automated detection quantitation matching and comparison of multiple 2 D DIGE gels e Image Loader importing images into an Oracle database e Administration Tools database administration maintenance and user access control e XML Toolbox exporting spot data from DIA or BVA modules for further downstream analysis GE Healthcare 6
17. DiGE approved ORDERING INFORMATION Immobiline DruStrip Gels e Maximize resolution loading capacity and reproducibility of first dimension IEF Product Quantity Code Number e Choose from a comprehensive range of overlapping Immobiline DryStrip gels 24 cm Immobiline DryStrip gels that cover narrow 1 pH unit Immobiline DryStrip pH 3 5 6 NL 24 cm La SE Di medium 3 5 pH units and broad 8 pH units pH ranges meg line DA Ope a a e Select the broad pH 3 11 NL gradient Immobiline DryStrip gel mobilne Pru ih PREE s m nes for fast and efficient screening to gain a broad overview of HA 5 ea O i eect i g g Immobiline DryStrip pH 3 11 NL 24 cm 12 17 6003 77 total protein disribution Immobiline DruStrip pH 3 5 4 5 24 cm 12 17 6002 38 e For more detailed studies or when using greater protein Immobiline DryStrip pH 4 0 5 0 24 cm 12 17 6002 39 loads choose from four new overlapping strips covering the Immobiline DryStrip pH 4 5 5 5 24cm 12 17 6002 40 OH 3 to 11 range Immobiline DryStrip pH 5 0 6 0 24cm 12 17 6002 41 e Higher resolution can be obtained using narrow range 1 pH Immobiline DryStrip pH 5 5 6 7 24 cm 12 17 6002 42 unit Immobiline DruStrip gels Immobiline DryStrip pH 3 7 NL 24cm 12 17 6002 43 m e Use matching IPG Buffers for optimized running conditions mmepl ine ipp aT Eem oa oO e Use IPG strips directly after reswelling without further Immobiline DrgStrip pe 9 2 cm i O ma nipu lation mm ins D
18. Dimension Immobiline DruStrip Gels continued TECHNICAL SPECIFICATIONS Related Products Code Number Refer To Etton IPGphor 3 Isoelectric Focusing Unit 11 0033 64 page 272 Multiphor I 18 1018 06 page 291 Immobiline DryStrip Kit 18 1004 30 page 292 IPG Buffers poge 271 IPGbox NEW 28 9334 65 page 271 IPGbox Kit NEW 28 9334 92 page 271 Introduction to Trap Kits for Protein Sample Preparation page 190 DeStreak Rehydration Solution 17 6003 19 page 307 DeStreak Reagent 17 6003 18 page 307 SELECTION GUIDE Immobiline DryStrip Gels Wy Strip length wh pHrange 2 4 6 8 10 12 24cm 18 cm 13 cm 11 cm 7 cm 5 5 0 NL e o o o 5 3 6 5 e o o e e O 53 2 ANE e e e e e O 3 11 NL a E e o e 3 5 4 5 a ae e LLJ 4 0 5 0 4 5 5 5 a e 5 0 6 0 uwi 5 5 6 7 3 7 NL mamana sind Sa e e 6 9 6 11 r 5 10 a a e 3 10 NL a E E Guidelines for choosing Immobiline DruStrip gels e Use shorter strips for fast screening or when onlu the most abundant proteins are of interest e Use longer strips for maximal resolution and loading capacity e To overview total protein distribution use pH 3 11 NL strips e For increased resolution between pH 5 7 use a nonlinear gradient pH 3 10 strip to distribute the proteins more evenly over the strip e Combine pH 3 7 NL and pH 6 11
19. E Comb various P Laminated rubber gasket 80 6174 62 F Cams 80 6174 24 Q Slotted rubber gasket 80 6174 43 SE 600 SE 400 Combs and Spacers All combs listed can be used in all SE 600 series and SE 400 e units Wells e Preparative combs with single or dual reference lanes Vol of sample per i No Thickness Width 1mm depth e Multichannel pipette compatible combs available x f e Spocers 16 cm and most SE 600 accessories con be used s allt p si E ith SE 400 Preparative Combs for SE 400 and SE 600 Series wi 1 1 0 75 121 6 90 4 1 80 6164 17 1 1 1 00 121 6 120 6 l 80 6164 36 Comb Accessory 1 2 0 75 113 6 85 4 1 80 6163 41 Adjustable comb back 1 80 6163 22 1 2 1 00 113 6 112 6 1 80 6165 60 Convert any 25 mm deep comb to 1 2 1 50 113 6 171 9 1 80 6163 79 10 or 15 mm depth Teflon Combs for SE 400 and SE 600 Series Length Thickness Width 10 0 75 83 6 2 80 6159 99 cm mm cm Quantity Code Number 10 1 00 a 8 3 1 80 6160 18 Spacers for Vertical Gel Units 10 1 50 83 12 4 1 80 6160 37 8 oms 2 2 80 6187 73 15 0 75 5 7 4 3 1 80 6161 13 8 1 00 2 2 80 6187 92 15 1 00 57 5 7 1 80 6161 32 8 1 50 2 2 80 6188 11 15 1 50 5 7 8 6 1 80 6161 51 16 0 75 2 80 6180 51 20 0 75 4 1 3 1 1 80 6161 70 16 1 00 1 2 80 6179 94 20 1 00 4 1 4 1 1 80 6161 89 16 1 00 2 2 80 6180 70 20 1 50 4 1 6 2 1 80 6162 08 16 1 50 1 2 80 6180 13 28t 0 75 27 2 0 1 80 6162 27 16 1 50 Z 2 80 6180 89 28t 1 00 2 7 2 7 1 80 6162 4
20. Lid for Gel Tray 1 18 1143 32 Syringe 1ml 1 18 1143 41 Waste Bottle and Tubing 1 18 1143 44 Tubing and Fitting 1 18 1143 63 Inlet Tubing Package 1 18 1143 65 Rinse Station 1 18 1143 31 Camera Calibration Foot 5 18 1143 33 White Sheet for Camera Calibration and Click and Pick 2 18 1143 51 Function Microplate Rack 18 1143 43 RS232 Cable 1 18 1143 45 Protective Hood 1 18 1149 17 For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To Ettan DALTtwelve Large Vertical System page 277 DALT Gel 12 5 26 x 20 cm 17 6002 36 page 277 DALT Buffer Kit for 12 precast gels 17 6002 50 page 277 Immobiline DryStrip Gels page 269 ImageMaster 2D Platinum v6 0 page 268 DeCyder 2 D Differential Analysis Software v6 5 page 265 ImageScanner III 28 9076 07 page 387 Typhoon Multislide Tray page 378 Ettan Digester page 281 Ettan Spotter page 282 Ettan Spot Picker Nonbacked Gel Kit page 280 TECHNICAL SPECIFICATIONS Sample source Polyacrylamide gels cast on glass GelBond PAGfilm backing or nonbacked gels Max gel size 280 x 250 mm 1 1 5 mm gel thickness compatibility Picker head diameter 1 4 2 0 mm gt 99 9 of spots transferred from gel to microplate Picking capacity 9600 protein spots pick list Process capacity 96 samples in less than 30 min Detection system compatibility Silver Coomassie fluorescence Picking efficiency Operating temperature 18 C to 30 C Max relative humid
21. Notes Multiple Mini Format 2 D Electrophoresis Using Precast ExcelGel and Multiphor lI Flatbed Electrophoresis System 80 6443 47 Multiple Mini Format 2 D Electrophoresis Using SE 600 Standard Vertical Electrophoresis System 80 6445 94 Poster 2 D Electrophoresis with Narrow Range Immobiline DryStrip Gels 18 1138 14 Video A Practical Introduction to 2 D Electrophoresis please inquire References 1 Gorg A et al Electrophoresis 9 531 546 1988 2 Corbett J et al Electrophoresis 15 1205 1211 1994 3 Blomberg A et al Electrophoresis 16 1935 1945 1995 For more information visit www gelifesciences com udo1125 3 O me OD T 259 2 D Electrophoresis SELECTION GUIDE 2 D Electrophoresis Tools for Proteome Studies Sample Preparation Kits and PlusOne Reagents see page 191 195 Ettan IPGphor 3 IEF System see page 272 273 Multiphor II Electrophoresis System 24cm u ss 18 cm Immobiline DryStrip Gels and IPG Buffer Saaz 13cm Speed and convenience see page 269 271 11cm 7 cm Choices for first dimension IEF Ettan DALTsix Large Vertical System Ettan DALT twelve Large Vertical System see page 274 275 see page 277 270 Ettan DALTsix Large Vertical System Multiphor Il System Ettan DALT twelve Large Vertical System see page 274 275 see page 291 see page 277 270 SE 600 Ruby Dual Cooled Vertical Unit see page 287 Multiphor Il Electrophoresis System
22. Quantity Code Number miniVE Vertical Electrophoresis Systems miniVE Complete 1 80 6418 77 Includes 3 rectangular glass plates 3 notched plates 2 gel modules lid lower buffer chamber 2 each 1 0 mm thick 10 well combs and 1 0 mm thick spacer sets Blot module 1 80 6418 96 Includes 3 Dacron sponges 1 4 thick 25 sheets of blotter paper 4 Gel Caster Complete 1 80 6146 12 Includes 5 rectangular glass plates 4 notched alumina plates 100 sheets wax paper spacesaver plate 5 filler sheets and set of filler plugs Rectangular glass plates 10 x 10 5 cm 5 80 6150 87 Notched glass plates 10 x 10 5 cm 5 80 6150 49 For pricing information visit www gelifesciences com orderonline Order combs and spacers separately miniVE Vertical Electrophoresis System is compatible with teflon combs spacers and glass and alumina plates for SE 260 Mini Vertical Units see page 286 Perform electrophoresis and electrotransfer using the compact miniVE Vertical Related Products Code Number Refer To anid aaa System shown here with two gel modules and optional blot EPS 301 Power Supply 18 113001 nage 304 EPS 2A200 Power Supply 80 6406 99 page 305 e Perform 1 D and 2 D electrophoresis and electrotransfer in Electrophoresis Reagents page 306 q single compact unit Rainbow Molecular Weight Markers IMPROVED page 316 e Cast run and blot mini gels in four streamlined components PlusOne Silver Staining Kit Protein 17 1150 01 page
23. SSCP DNA fragment t et GenePhor Multiphor II analysis Isoelectric focusing Multiphor II carrier ampholyte Adapter 18 1129 59 required for connecting 4 mm high voltage leads on gel units to power supplies with 2 mm output jacks t Adapter 18 1129 58 required for connecting 2 mm high voltage leads on gel units to power supplies with 4 mm output jacks t See Chapter 6 e Recommended e Possible but not recommended may exceed safety limits of the instrument Has limited voltage range for some applications e Has limited current range for some applications EPS 301 300 V 400 mA 80 W maximum Constant voltage or constant current modes Single unit increments in settings and read outs for precision and reproducibility Suitable for submarine mini vertical and standard vertical gel electrophoresis as well as semidry and mini tank blotting applications EPS 601 600 V 400 mA 100 W maximum Constant voltage constant current and constant power modes Single unit increments in settings and read outs for precision and reproducibility Stores and recalls three protocols Suitable for general applications including mini and standard sized vertical gels and many blotting applications EPS 1001 1000 V 400 mA 100 W maximum Constant voltage constant current and constant power modes Single unit increments in settings and read outs for precision and reproducibility Stores and recalls thr
24. Tris pH 8 8 t Reswell in choice of carrier ampholyte composition using PhastGel cassette All PhastGel IEF gels contain Pharmalyte carrier ampholytes Blotting Products For main product entries see Chapter 9 GenePhor DNA Separation System and Precast Gels For main product entries page 238 Introduction to Nucleic Acid Electrophoresis Units SELECTION GUIDE BY TECHNICAL OVERVIEW Nucleic Acid Electrophoresis Units Separation Distance cm Maximum Maximum at Maximum Separation Gel Size Buffer Volume Model Key Application Features Samples Gel Samples Distance cm W x L cm ml Mini Format HE 33 Coolant filled base for fast large sample 32 4 10 7x 10 150 250 number runs HE 99X Basic large format unit for large sample 1000 1200 Gel Tray numbers 60 4 10 15x10 Gel Tray ll 60 6 15 15x15 Gel Tray III 60 8 20 15 x 20 For general information concerning electrophoresis of nucleic acids see Technical Appendix 20 well combs offer 9 mm spacing for multichannel pipette loading For more information visit www gelifesciences com GE Healthcare Nucleic Acid Electrophoresis HE 33 Mini Submarine Unit HE 33 Mini Submarine Unit gives enhanced passive cooling for faster runs e Coolant insulated buffer chamber runs 7 x10 cm agarose gels in less than 20 min e Convenient leak proof casting trays mean fast gel casting without tape e UV transparent gel tray offers convenient gel ha
25. a pooled internal standard Proteomics 3 36 44 2003 2 Yan J X et al Fluorescence 2 D Difference Gel Electrophoresis and mass spectrometry based proteomic analysis of Escherichia coli Proteomics 2 1682 1698 2002 3 Unlu M et al Difference gel electrophoresis a single method for detecting changes in protein extracts Electrophoresis 18 2071 2077 1997 4 Tonge R P et al Validation and development of fluorescence two dimensional difference gel electrophoresis proteomics technology Proteomics 1 377 396 2001 For more information visit www gelifesciences com ydoj 9a 3 O x OD T 265 n Q O O O i jad Q LL 266 2 D Electrophoresis 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE DeCyder Extended Data Analysis EDA Software v1 0 Importing gt qnd linking BVA workspaces BVA WS from the DeCyder database Setup Calculations Results or Filtering Interpretation and possibly normalization Calculations or Interpretation Creating new sets and combining sets optional Calculations or Interpretation Importing MS data Analysis workflow in DeCyder EDA e Offers advanced statistical analysis of proteomics data sets in a simple to use format e Employs multivariate analysis and sophisticated clustering methods to uncover patterns and relationships in expression data e Identifies proteins that discriminate betwee
26. a 100 bp fragment resolves to 20 or more readily distinguishable bands each separated by k fe a tsat k a Lil a L L ki ee L ws h 0 eesssim uma j e 4 100 Base Pair Ladder visualized by 100 bp increments when run on a 1 5 agarose gel m HUOT CENCE WiN EINAN Drome e Can be visualized by ethidium bromide staining or by A and autoradiography B Panel A oad hy aft dielabelingl 2 ug of Ladder was analyzed on a autora lograp y tatter ra lo al ING O 1 5 agarose gel with TBE e Bands stain uniformly with ethidium bromide except for the or containing ethidium bromide as 800 bp band which is approximately twice as intense as the running buffer Ponel B 2 ug of other bands 100 Ladder was labeled with a 32P dCTP ee ee df tatina DNA O using a fill in reaction e Not designed tor quantitating content In a sample Approximately 0 5 ug of labeled O Ladder was analyzed on q 1 5 agarose gel oO N N KiloBase DNA Marker 1 2 3 4 5 6 ORDERING INFORMATION Product Quantity Code Number KiloBase DNA Marker 50ug 27 4004 01 For pricing information visit www gelifesciences com orderonline Related Products Refer To Nucleic Acid Electrophoresis page 300 e For quick and precise sizing of double stranded DNA fragments from 500 bp to 10 0 kb on agarose gels e Supplied at 0 5 ug ul in TE buffer pH 8 0 in sufficient quantit
27. allow the labeling of 5 ug protein per labeling reaction compared to 50 ug with the minimal dyes The maleimide reactive group of the saturation dyes covalently bonds to the thiol group of cysteine residues of proteins via a thioether linkage For more information visit www gelifesciences com DIGE approved ORDERING INFORMATION Product Quantity Code Number CyDye DIGE Fluor Cy2 minimal dye 5nmol 25 8010 82 CyDye DIGE Fluor Cy3 minimal dye 5nmol 25 8010 83 CyDye DIGE Fluor Cy5 minimal dye 5nmol 25 8010 85 CyDye DIGE Fluor Cy2 minimal dye 10 nmol 25 8008 60 CyDye DIGE Fluor Cy3 minimal dye 10 nmol 25 8008 61 CyDye DIGE Fluor Cy5 minimal dye 10 nmol 25 8008 62 CyDye DIGE Fluor Cy2 minimal dye 25 nmol RPK0272 CyDye DIGE Fluor Cy3 minimal dye 25 nmol RPK0273 CyDye DIGE Fluor Cy5 minimal dye 25 nmol RPK0275 CyDye DIGE Fluor minimal dye labeling 2nmol 28 9345 30 kit NEW DIGE Trial Pack incl CyDye DIGE Fluor 1 28 9373 73 minimal dye labeling kit 2 nmol 1 month free DeCyder 2 D trial license NEW CyDye DIGE Fluor minimal labeling kit 5nmol 25 8010 65 CyDye DIGE Fluor Labeling Kit for Scarce 1 25 8009 83 Samples CyDye DIGE Fluor Labeling Kit for Scarce 1 25 8009 84 Samples and Preparative Gel Labeling CyDye DIGE Fluor Preparative Gel Labeling for Scarce Samples Cy3 saturation dye NEW 400 nmol 28 9366 83 For pricing information visit www gelifesciences com orderonline Related Products Code Number
28. ceramic cooling plate for improved resolution and speed at high voltages e Adjustable electrodes allow use of different sized gels The modular design of Multiphor II gives it the flexibility to handle virtually any flatbed electrophoretic technique It is particularly well suited for ultra thin gels 0 1 0 5 mm on glass or plastic supports up to 20 x 26 cm In addition to accommodating gels of different sizes the electrodes make secure uniform contact with various types of buffer strips eliminating the need for large volumes of liquid buffers Buffer strips can be positioned and held in place using Multiphor I Buffer Strip Positioner ExcelGel and Ampholine Precast Polyacrylamide Gels These gels are available in formulations for denaturing and native protein separations isoelectric focusing and high resolution DNA separations For a complete listing of precast gels and buffer kits available for Multiphor IlI System see page 294 Multiphor II Electrophoresis Unit The unit is supplied with a ceramic cooling plate strip electrodes and an electrode holder The basic unit runs a variety of precast gels For casting special application gels a casting kit and accessories are required see page 292 For performing first dimension IEF separations use Immobiline DryStrip Gels see page 269 which require the use of Immobiline DryStrip Kit see page 292 For performing semi dry blotting use NovaBlot Kit see page 293 GE Healthc
29. for sample volumes from 5 17 ul The gradient gels use sample application strips 26 samples up to 40 ul 52 samples up to 20 ul paper pieces or sample droplets All ExcelGel SDS Homogeneous and 8 18 gradient gels have a 33 mm stacking zone and a 77 mm separating zone ExcelGel gradient gels are recommended for use with Immobiline DryStrip gels see page 269 For information on ExcelGel for 2 D electrophoresis see page 297 Gel description Dimensions mm W x L x T Gradient Homogeneous Homogeneous Homogeneous 8 18 7 5 12 5 15 Separation range M x 103 15 200 35 200 15 40 3 8 245 x 110 x 0 5 250 x 110 x 0 5 250 x 110 x 0 5 250 x 110 x 0 5 A Tris HAC pH 6 4 B As in A plus 30 ethulene glucol Stacking zone T C 5 3 5 3 6 3 7 5 3 Separating zone T C 8 18 3 75 3 12 5 2 15 3 Buffer A A A B Storage temperature C 20 30 4 8 4 8 4 8 Buffers oas II Buffer Strip Positioner for ExcelGel Z buffer strip IPG strip C Anode buffer strip 24x 11 cm gel o Anode buffer strip 24x 18 cm gel GE Healthcare Multiphor II Buffer Strip Positioner is a frame with slots that sits on top of ExcelGel precast gel after the gel is placed on Multiphor II cooling plate The slots in the positioner facilitate placement of the buffer strips for electrophoresis and hold the strips securely in place during the run ORDERING INFORMATION Product Multiphor II Buffer Strip Positioner complete
30. fragment sizes can be found in the Selection Guide see page 320 GE Healthcare For more information visit www gelifesciences com 321
31. free highly purified proteins The markers may be run in parallel with the experimental samples on isoelectric focusing gels These markers are not prestained The precisely defined pl of the markers allows a permanent and accurate calibration of sample components pH gradients and pl can be measured to within 0 01 0 05 pH unit The progress of the focusing experiment can be monitored visually by the focusing of methyl red or Cytochrome c Carbamylyte Calibration Kit is intended for use with urea denaturing IEF gels and is particularly suitable as an internal standard in 2 D electrophoresis Carbamylyte standards resolve into a chain of spots all of the same molecular weight The kit contains three different molecular weight standards TECHNICAL SPECIFICATIONS Carbamylyte Calibration Kit Protein pl Range No of Vials Creatine phosphokinase 34 spots 4 9 7 1 4 Carbonic anhydrase 20 spots 4 8 6 7 4 Glyceraldehyde 3 phosphate 4 7 8 3 2 dehydrogenase 34 spots For more information visit www gelifesciences com udo1125 3 O me D T 319 IEF SDS PAGE amp 2 D Electrophoresis DNA Molecular Weight Markers SELECTION GUIDE DNA Markers D l l l l D l l l 5 88 SELECTION GUIDE DNA Markers Precise Sizing Digest of Natural DNAs KiloBase 50 Base Pair Ladder 100 Base Pair Ladder DNA Marker DRigest III 27 4005 01 27 4007 01 27 4004 01 27 40
32. from 15000 to 150000 Each pack contains ECL DualVue markers 125 ul and D S protein HRP conjugate 50 ul sufficient for 25 standard gel N loadings N TECHNICAL SPECIFICATIONS A Membrane ECL DualVue Western Blotting Markers after electrophoresis on a 4 20 SDS PAGE slab gel and transfer onto Hybond ECL nitrocellulose membrane Prestained indicator proteins are shown as a photograph of the membrane A together with tagged recombinant proteins after detection with ECL Western Blotting Detection Reagents as a photograph of the film B Electrophoresis performed for 45 min at 200 V 1 min film exposure GE Healthcare For more information visit www gelifesciences com 317 IEF SDS PAGE amp 2 D Electrophoresis Protein Molecular Weight Markers qnd pl Markers Amersham ECL Western Blotting Molecular Weight Markers e Biotinylated marker proteins with defined molecular weights in the range 14400 to 97000 enable molecular weight determination in Western blots e Markers when incubated with Streptavidin HRP are visualized using a luminol based light producing reaction generated with ECL detection reagents e The protein mix total concentration 1 mg ml is supplied in 50 glycerol and requires dilution in SDS PAGE loading buffer Storage Is at 15 C to 30 C TECHNICAL SPECIFICATIONS n Q O Z or O gt Q LL Protein Molecular Weight Standards e Protein molecular weight standards in
33. handles gel cassettes up to 10 x 10 5 cm W x H which increases the separation length by more than 30 over conventional 10 x 8 cm gels In addition miniVE is compatible with the glass plates combs and spacers of SE 260 Mini Vertical units For gradient gels mini Vertical Gel Casters can produce up to four identical gels at one time The Right Power Supplies EPS 301 is the power supply of choice for the miniVE System With ratings of 300 V and 400 mA it is capable of both running and blotting gels For faster transfers when blotting four gels at a time use the EPS 2A200 for its higher current capability Load Clamp and Cast The gel module is simultaneously a casting stand and an upper buffer chamber with integrated clamps Simply assemble the gel sandwich of plates and spacers clamp it onto the upper buffer chamber then pivot the sealing gasket and cast the gel After polymerization simply release the bottom seal and drop the assembly into the tank for running No extra parts are needed and no unclamping until the run is finished Ld Mm gt UE l H d i For more information visit www gelifesciences com miniVE System Blot Module can accept two gel membrane stacks simultaneously and cast Detection of E coli GroEL following Western transfer in miniVE Blot Module Two fold serial dilutions of a crude protein extract we
34. life Precast gel cassettes improve handling efficiency and throughput Gels can be quickly loaded and unloaded with minimal hands on time GE Healthcare For more information visit www gelifesciences com 277 n QO O Z O O i gt Q LL 278 2 D Electrophoresis Second Dimension Ettan DALTtwelve Large Vertical System continued TECHNICAL SPECIFICATIONS Precast Ta load easily into Ettan DALT Precast Gel Cassettes no glass plate assembly required After a precast gel is loaded the IPG strip is added sealed into position and the cassette is placed into the Ettan DALTtwelve Separation Unit DALTtwelve Gel Caster For added flexibility DALTtwelve Gel Caster is available for casting up to 14 gels simultaneously Regular and low fluorescence glass plates are available for use with the DALT Gel Casting Cassette Low fluorescence glass plates ensure high sensitivity when using fluorescent dyes Gradient gels can be prepared using DALT Gradient Maker Multiple Gel Staining with Ettan Staining Tray Set After the second dimension run is complete the large format gels can be stained using DALT Staining Tray Set and PlusOne Silver Staining Kit Protein This combination ensures reliable detection with high sensitivity Cast gels using DALTtwelve Gel Caster and choose between low fluorescence or regular glass plates DALT Staining Tray Set and PlusOne Silver Staining Kit Protein deliver rel
35. off or phase end based on time volt hour milliampere hour e Current rating of up to 400 mA to handle both electrophoresis and basic blotting applications e Current resolution of 1 uA for Immobiline DryStrip and other IPG separations EPS 2A200 EPS 2A200 Power Supply high current for all types of electrophoretic blotting Power Supply Adapters 4 mm sheathed output jacks 2 mm high voltage output jack ao found on all current found on all current a Amersham Biosciences Amersham Biosciences power supplies rated at power supplies rated at S voltages less than 1000V voltages of 1000 V Y Q and more NY x o z Instrument Leads X S Y k Sheathed 4 mm male plug installed on all current 18 1129 59 Amersham Biosciences electrophoresis units rated at voltages less than 1000V no adapter necessary _ j A Sheathed 2 mm male plug included with all current 18 1129 58 Amersham Biosciences electrophoresis units rated 1000V and more no adapter necessary B GE Healthcare ORDERING INFORMATION Product Quantity Code Number EPS 3501 XL Power Supply 1 18 1130 05 For pricing information visit www gelifesciences com orderonline Related Products Multiphor II Refer To page 291 Code Number 18 1018 06 Supported by ORDERING INFORMATION Product Quantity Code Number EPS 2A200 Power Supply 1 80 6406 99 For pricin
36. the range 6500 205000 e Sufficient material for 200 gel lanes e Bands can be visualized by using Deep Purple Total Protein Stain Coomassie Brilliant Blue or silver staining e The protein mix total concentration 60 ug ml is supplied in storage solution containing 50 v v glycerol Store at 15 C to 30 C ORDERING INFORMATION Product Quantity Code Number ECL Western Blotting Molecular Weight Markers1 pack 25 loadings RPN2107 ECL Protein Molecular Weight Markers with 1pack RPN2280 Strep HRP For pricing information visit www gelifesciences com orderonline Related Products Refer To Streptavidin Enzyme Conjugates page 360 Amersham Hybond ECL page 352 Amersham Hybond P page 352 Amersham Hyperfilm ECL page 390 Amersham ECL Western Blotting Detection Reagents page 357 Amersham ECL Plus Western Blotting Detection Reagents page 356 miniVE Vertical Electrophoresis System page 283 Quantity Code Number 400 ul RPN5800 For pricing information visit www gelifesciences com orderonline TECHNICAL SPECIFICATIONS 318 For more information visit www gelifesciences com GE Healthcare IEF SDS PAGE amp 2 D Electrophoresis Protein Molecular Weight Markers and pl Markers 14C Protein Molecular Weight Markers e Carbon 14 radiolabeled marker proteins enable molecular weight determination of the sample on Western blots e The Rainbow labeled version also allows visual monitoring of band migration in the
37. up to six lab cast or precast gels 26 x 20 cm simultaneously e Use with 18 or 24 cm Immobiline DryStrip gels e Simple to use 1 0 mm or 1 5 mm pre assembled hinged gel cassettes e Innovative design simplifies loading and unloading e Built in ceramic heat exchanger and buffer circulation pump ensure efficient cooling e Improved Upper Buffer Chamber in combination with updated protocols will optimize results under the widest variety of run conditions Ettan DALTsix Electrophoresis Unit runs up to six large format 26 x 20 cm second dimension polyacrylamide gels in just 4 5 h The gels accept first dimension 18 or 24 cm Immobiline DryStrip gels IPG strips from both Ettan IPGphor and Multiphor Il isoelectric focusing systems Ettan DALTsix system is modular in design The system components include Ettan DALTsix Electrophoresis Unit Gel Caster and Gradient Maker an EPS 601 Power Supply and MultiTemp IIl Thermostatic Circulator All Ettan DALTtwelve Electrophoresis System accessories including blank cassette insert separator sheets filler sheets cassette rack and staining tray set are compatible with Ettan DALTsix units The DALTsix Gradient Maker is designed to produce linear convex and concave exponential gradients of solutions ranging in volume from 200 to 1000 ml A sliding valve at the bottom quickly connects or disconnects the chambers For more information visit www gelifesciences com OGE approved ORDERIN
38. 0 lor2 Dual Gel Caster Dual Gel Caster 80 6175 00 80 6175 00 2 4 Gel Caster 4 gels 80 6191 34 2 10 Multiple Gel Caster 80 6182 79 GE Healthcare Standard Format Vertical Units ORDERING INFORMATION Product Quantity Code Number Sturdier Vertical Slab Electrophoresis Units for 18 x 16 cm plates SE 400 Vertical Unit Complete comb and 2 spacers 1 5 mm thick 1 Includes One set of glass plates 18 x16 cm 2 clamp assemblies and 2 cams 15 well 80 6155 24 Accessories SE 600 SE 400 Gel Casters see this page those for the SE 600 series see page 288 Replacement Parts Blank silicone rubber gasket for casting stand Lower buffer chamber casting stand Upper buffer chamber Lid with electrodes for SE 400 16 cm High voltage safety lead set Slotted silicone rubber gasket for upper buffer chamber Combs spacers glass plates and accessories for SE 400 16 cm unit are the same as 1 1 1 1 1 1 80 6155 43 80 6155 62 80 6156 76 80 6156 95 80 6157 14 80 6177 09 For pricing information visit www gelifesciences com orderonline Related Products EPS 601 Power Supply Rainbow Molecular Weight Markers IMPROVED SE 100 Plate Washer and Storage Unit Electrophoresis Reagents Code Number 18 1130 02 80 6116 29 Refer To page 304 page 316 page 290 page 306 ORDERING INFORMATION Product Quantity Code Number Gel Casters for 1 or 2 gels Dual Gel
39. 0 6498 76 Lid adaptor for IPGphor 3 1 28 4008 41 Cleaning brush 1 80 6505 98 Spirit level 1 80 6194 19 Forceps SS 1 80 6506 17 Ettan IPGphor Manifold Light Ettan IPGphor Manifold Light Complete 1 11 0026 88 Ettan IPGphor Manifold Polymeric Tray 1 11 0025 80 Strip holders 24 cm Strip Holder Complete 6 80 6469 88 18 cm Strip Holder Complete 6 80 6416 68 13 cm Strip Holder Complete 6 80 6416 49 11 cm Strip Holder Complete 6 80 6416 30 7 cm Strip Holder Complete 6 80 6416 11 24 cm Strip Holder Complete 1 80 6470 07 18 cm Strip Holder Complete 1 80 6417 44 13 cm Strip Holder Complete 1 80 6417 25 11 cm Strip Holder Complete 1 80 6417 06 Sample Cup Cup Loading Strip Holder 50 80 6459 81 Cleaning Solution Strip Holder 950ml 80 6452 78 Strip holder covers Strip Holder Cover 7 cm 2 80 6455 44 Strip Holder Cover 11 cm 2 80 6455 63 Strip Holder Cover 13 cm 2 80 6455 82 Strip Holder Cover 18 cm 2 80 6456 01 Strip Holder Cover 24 cm 2 80 6479 76 Accessory Equilibration Tube Set 12 80 6467 79 For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To Immobiline DryStrip Gels page 269 CyDye DIGE Fluors page 264 Introduction to Trap Kits for Protein Sample Preparation page 190 DeStreak Rehydration Solution 17 6003 19 page 307 DeStreak Reagent 17 6003 18 page 307 Ettan IPGphor 3 Control Software enables creation storage and editing of protocols Recommended run
40. 03 19 DeStreak Reagent 1ml 17 6003 18 For pricing information visit www gelifesciences com orderonline Related Products Refer To Introduction to Trap Kits for Protein Sample Preparation page 190 Immobiline DryStrip Gels page 269 Mouse liver proteins 80 ug run on a 24 cm long Immobiline DryStrip gel pH 6 9 The IPG strip was rehydrated in 1 IPG Buffer pH 6 11 8 M urea 0 5 CHAPS 10 mM DTT without DeStreak Rehydration Solution Poor resolution and horizontal streaking are evident on the resulting 2 D gel Same sample sample load and Immobiline DryStrip gel but with rehydration of the IPG strip in DeStreak Rehydration Solution with 1 IPG Buffer Horizontal streaking is reduced improving the overall quality of the resulting 2 D pattern DeStreak Rehydration Solution contains optimized concentrations of urea thiourea CHAPS and DeStreak Reagent The solution is ready for use after addition of the appropriate IPG Buffer DeStreak Reagent is also available separately ina 1 ml pack size For more information visit www gelifesciences com udo1125 3 O me OD T 307 p T e a O a an O O ad jd Q O LLJ 308 Electrophoresis Standards and Reagents PlusOne Acrylamide PlusOne Acrylamide PAGE contains extremely low concentrations of impurities that can interfere with the polymerization reaction It is especially suitable for SDS and native PAGE techniques PlusOne Acry
41. 20 pl 5 2 10 3 Carbonic anhydrase B bovine 5 85 Carbonic anhydrase B human 6 55 Muoglobin acidic 6 85 Myoglobin basic 135 Lentil lectin acidic 8 15 Lentil lectin middle 8 45 Lentil lectin basic 8 65 Trypsinogen 9 30 Cytochrome c 10 25 Measured at 24 C GE Healthcare ORDERING INFORMATION Product Quantity Code Number 14C Methylated Protein Molecular Weight Markers 37 kBq 1 uCi CFA626 1UCI 14C Methylated Protein Molecular Weight Markers 185 kBq 5 uCi CFA626 5UCI 14C Methylated Protein Molecular Weight Markers 37 kBq 1 uCi CFA645 1UCI Rainbow 14C Methylated Protein Markers 37 kBq 1 uCi CFA755 1UCI Rainbow 14C Methylated Protein Markers 37 kBq 1 uCi CFA756 1UCI For pricing information visit www gelifesciences com orderonline Store at 20 C Related Products Refer To Amersham Hybond ECL page 352 Amersham Hybond P page 352 ORDERING INFORMATION Product Quantity Code Number Broad pl Kit pH 3 10 10 vials 325 ug vial 17 0471 01 Low pl Kit pH 2 5 6 5 10 vials 280 ug vial 17 0472 01 High pl Kit pH 5 10 5 10 vials 280 ug vial 17 0473 01 Carbamylyte Calibration Kit 1kit 17 0582 01 For pricing information visit www gelifesciences com orderonline Broad Low and High pl kits are not for use in denaturing urea systems Store at 4 C Refer To page 291 Code Number 18 1018 06 Related Products Multiphor II pl markers are lyophilized mixtures of stable salt
42. 250g 17 1300 01 PlusOne Acrylamide IEF 40 Solution 11 17 1301 01 PlusOne ReadySol IEF 40 T 3 C 11 17 1310 01 For pricing information visit www gelifesciences com orderonline Solution Solutions of acrylamide are convenient virtually eliminating the risk of acrylamide dust in the air ReadySol ReadySol a solution of acrylamide and bisacrylamide is convenient and fast with minimal preparation and handling ReadySol ensures a reproducible solution composition ORDERING INFORMATION Product Quantity Code Number Agarose IEF 10g 17 0468 01 Agarose NA 100g 17 0554 02 Agarose NA 1kg 17 0554 03 Agarose Prep 50g 80 1130 07 For pricing information visit www gelifesciences com orderonline TECHNICAL SPECIFICATIONS Agarose Type Electroendosmosis Gel Strength Gelling Temp Melting Temp M Kobe 1 5 C Agarose IEF 0 00 gt 1000 40 43 Agarose NA 0 10 2400 34 37 Agarose Prep lt 0 10 gt 200 lt 30 65 1 agarose PlusOne Amberlite IRN 150L Amberlite IRN 150L is a homogeneous mixture of strong cationic and strong anionic resins It is used to remove trace ionic impurities from nonionic chemicals such as urea For more information visit www gelifesciences com ORDERING INFORMATION Product PlusOne Amberlite IRN 150L Quantity Code Number 500g 17 1326 01 For pricing information visit www gelifesciences com orderonline Store at room temperature GE Healt
43. 4 8 tee 1 80 6139 09 15 0 75 2 9 2 2 1 80 6139 47 15 1 00 2 2 9 1 80 6139 66 15 1 50 2 9 4 4 1 80 6139 85 18 1 00 2 9 29 1 80 6140 04 1 1t 0 75 68 5 50 3 5 1 80 6141 56 1 1t 1 00 68 5 65 5 1 80 6141 75 1 1t 1 50 68 5 100 7 5 1 80 6141 94 Thickness Length mm cm Quantity Code Number Spacers 0 75 8 2 80 6137 95 1 00 8 2 80 6138 14 1 50 8 2 80 6138 33 0 75 10 5 2 80 6149 92 1 00 10 5 2 80 6150 11 1 50 10 5 2 80 6150 30 Product Quantity Code Number Glass and Alumina Plates 10 x 8 cm W x L for SE 250 Notched alumina plates 10 80 6136 43 Rectangular glass plates 10 80 6136 81 Glass and Alumina Plates 10 x 10 5 cm W x L for SE 260 Notched alumina plates 5 80 6150 68 Rectangular glass plates 5 80 6150 87 For pricing information visit www gelifesciences com orderonline Microplate spacing t Preparative reference wells SELECTION GUIDE Gel Casting Options Mini Vertical unit No of gels miniVE SE 250 SE 260 lor2 Dual Gel Caster Dual Gel Caster Dual Gel Caster 80 6146 50 80 6146 50 80 6146 50 2 4 4 Gel Caster 4 Gel Caster 4 Gel Caster 80 6146 12 80 6151 06 80 6146 12 4 10 Multiple Gel Caster 80 6142 51 ORDERING INFORMATION Product Quantity Code Number Caster Accessories and Replacement Parts Wax paper sheets for 10 x 10 5 cm 100 80 6145 55 Wax paper sheets for 10 x 8 cm 100 80 6135 67 Filler sheets for 10 x 10 5 cm 5 80 6145 93 Foam gasket 4 5 mm o d x 61 cm long 1 80 613
44. 5 OGE approved ORDERING INFORMATION Product Quantity Code Number DeCyder 2 D Differential Analysis Software v6 5 1 28 4012 01 preinstalled network including PC and single concurrent network user license DeCyder 2 D Differential Analysis Software v6 5 one 1 11 0035 82 network user license DeCyder 2 D Differential Analysis Software v6 5 four 1 11 0035 81 additional network user licenses Other packages available on request For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To Typhoon Variable Mode Imager page 376 Typhoon Trio 63 0055 87 page 376 DeCuder Extended Data Analysis EDA Software v1 0 page 266 Ettan DIGE Imager including installation kit 63 0056 42 page 383 Ettan IPGphor 3 Isoelectric Focusing Unit 11 0033 64 page 383 ImageMaster 2D Platinum v6 0 page 268 CyDye DIGE Fluors page 264 SCF Gta X Q T Q ta Anaan W DeF emi aj ea er eee Grk ipa a w J T h GY Aen il lt E r A s j A A EEFIN EARLE Peay hae fare BELE sa lam M Hei mna He sie TL kin Fe kos il hat J lal i _ oo wes Pir F Bak gt za sa Ge asss eee al E E BVA user interface is used to detect and statistically quantitate differences between samples References 1 Alban A et al A novel experimental design for comparative two dimensional gel analysis two dimensional difference gel electrophoresis incorporating
45. 5 VAC 50 60 Hz 120 W 50 60 Hz 140 W 50 60 Hz 140 W 50 60 Hz 260 W 47 63 Hz 325 W Safety features overload short circuit protection floating output overload short circuit protection ground leakage detection no load overload short circuit protection ground leakage detection no load overload short circuit protection ground leakage detection no load overload short circuit detection no load detection detection detection detection Dimensions mm 250 x 215 x 95 250 x 310 x 95 250 x 310 x 95 250 x 310 x 95 110 x 270 x 220 weight kg 3 0 3 8 3 8 3 8 2 2 Page 304 304 304 305 305 Power Supplu Adapters see page 305 GE Healthcare For more information visit www gelifesciences com 303 n Q O S O O Q LL 304 Power Supplies EPS Power Supplies continued SELECTION GUIDE BY APPLICATION Power Supplies Application EPS EPS EPS EPS EPS Electrophoresis 301 601 1001 3501 XL 2A200 Equipment Ultrathin flatbed PAGE et Multiphor II SDS or Native Standard SDS PAGE e e e SE 400 600 Series 16 cm long Mini SDS PAGE e e e miniVE SE 260 High volume Ettan DALTsix SDS PAGE Submarine o e x HE Series DNA sequencing Western blotting e a TE 22 mini tank Western blotting e e TE 62 standard tank Western blotting o e o TE 70 Series semi dry
46. 6 24 0 75 2 2 80 6190 58 28t 1 50 2 7 4 1 1 80 6162 65 24 1 00 2 2 80 6190 77 s ae I oa Aen OGO Oe For pricing information visit www gelifesciences com orderonline These combs are 25 mm deep adjustable to 10 or 15 mm For pricing information visit www gelifesciences com orderonline t Comb depth 15 mm all others 25 mm 288 For more information visit www gelifesciences com GE Healthcare SDS PAGE IEF SE 400 Vertical Unit SE 400 Vertical Unit is a durable and economical unit for standard 16 cm long slab gels e Economical safe vertical slab electrophoresis units with many of the conveniences of SE 600 series units e Rugged injection molded construction for easy assembly and durability in high use labs e Built in casting stand seals sandwiches leak free without tape e Plates clamps spacers and combs are interchangeable with SE 600 series units SE 400 Vertical Units run one 16 cm gel or two gels with an accessory divider plate Its low cost makes the SE 400 appropriate for labs requiring multiple units when temperature control is not crucial The unit base is also a convenient casting stand for single gels To cast multiple gels for one or more units use the multiple gel casters SE 600 SE 400 Gel Casters m q Cost multiple gels qt once using Mini Verticol Gel Casters SELECTION GUIDE Gel Casters SE 600 SE 400 Vertical unit No of gels SE 600 Rubu SE 660 or SE 40
47. 60 01 page this page page 321 page 321 page 321 500 2000 10000 231301 450 1900 8000 9416 400 1800 6000 6557 350 1700 5000 43611 300 1600 4000 2322 250 1500 3000 2027 200 1400 2500 1353 150 1300 2000 1078 100 1200 1500 872 VN 50 1100 1000 603 ET 1000 500 564 t D 900 310 800 281 O 792 271 pa aa 700 234 600 194 O 500 1254 400 118 Y 300 72 O 200 uJ 100 Not necessarily the largest size fragment possible only the largest that is readily distinguishable t Cos ends located on these bands Very faint band SELECTION GUIDE DNA Markers Marker Code Number Recommended Gel Loading Amount ug lane Heat Before Loading 27 4005 01 2 agarose 6 polyacrylamide 2 0 No 27 4007 01 1 5 agarose 2 0 No 27 4004 01 0 8 agarose 0 5 No 27 4060 01 1 agarose 0 5 1 0 60 C to 65 C for 2 min 50 Base Pair Ladder ORDERING INFORMATION Product Quantity Code Number 50 Base Pair Ladder 200ug 27 4005 01 _ _ _ w k k k ku k T 320 50 Base Pair Ladder and four DNA samples were analyzed on a 2 agarose gel with TAE as the running buffer Bands were visualized under UV light after staining with ethidium bromide Lane 1 50 Base Pair Ladder lane 2 137 bp PCR product lane 3 plasmid digested to completion with Aval to release a 250 bp fragment lane 4 504 bp PCR product lane 5 887 bp PCR product lane 6 50 Base Pair Ladder Fo
48. 7 19 Space saver plate for 10 x 10 5 cm 1 80 6146 31 Sealing gasket set for Dual Gel Caster 2 80 6146 69 Casting clamp assembly for Dual Gel Caster 1 80 6147 07 1 Silicone rubber filler plug set for 4 Gel Caster 10 x 8 cm 80 6151 44 and 10 x 10 5 cm For pricing information visit www gelifesciences com orderonline GE Healthcare SDS PAGE IEF SE 600 Ruby and SE 660 Dual Cooled Vertical Units An En Z NE Ta SE 600 Ruby for running 8 or 16 cm long gels e Run denaturing and native polyacrylamide 2 D electrophoresis agarose and nucleic acid gel electrophoresis e Maintain temperature between 1 and 45 C with built in heat exchanger for faster runs with no sacrifice in quality e Run two gels simultaneously or run four gels using accessory divider plates e Cast single or multiple gels in the two place casting stand or in the four gel or ten gel accessory casters e Perform second dimension separations using two 7 cm IPG strips side by side allowing eight second dimension separations at once e Cast and run wide mini gels using 8 cm glass plates and clamps The key to outstanding reproducible electrophoresis results is temperature control Gels unequally cooled on their two faces produce slanted bands appearing as broad or double bands in stained gels SE 600 Ruby ensures uniform heat in gels because firstly the gels are completely immersed in the lower buffer for uniform heat transfe
49. 80 6197 61 SG 30 Gradient maker 30 ml total volume 1 80 6197 80 SG 50 Gradient maker 50 ml total volume 1 80 6197 99 SG 100 Gradient maker 100 ml total volume 1 80 6196 09 SG 500 Gradient maker 500 ml total volume 1 80 6198 18 Accessories and Replacement Parts White 3 mm outlet fitting for SG 15 SG 30 SG 50 and 1 80 6196 85 SG 100 Valve salt gradient push pull for SG 500 1 80 6198 56 Outlet fitting red 4 mm for SG 500 4 80 6226 30 For pricing information visit www gelifesciences com orderonline N chromatography elution and sucrose cesium and For gradients up to 2 2 use to DALT Gradient Maker see T preparative zonal centrifugation page 277 Q e Choose from five sizes between 15 ml and 500 ml total gt volume O Ring stand support rod included with the SG 15 SG 30 SG 50 a an d and SG 100 O u SELECTION GUIDE Gradient Volumes for Gel Casters D Approximate Gradient Volumes Electrophoresis No of Gel Caster Gel Thickness Unit Gels 0 75 mm 1 0 mm 1 5 mm LLJ SE 600 SE 400 10 Multiple Gel Coster 18 x 16 cm 80 6182 79 222 ml 278 ml 390 ml SE 600 SE 400 4 Gel Caster Kit 4 Gels 18 x 16 cm 80 6191 34 63 ml 85 ml 130 ml SE 260 miniVE 4 4 Gel Caster 10 x 10 5 cm 80 6146 12 29 ml 37 ml 54 ml SE 100 Plate Washer and Storage Unit SE 100 Plate Washer and Storage Unit simplifies glass plate handling Soak rinse and store multiple gel plates with convenient rack and covered ta
50. Backing 34 x 44 cm Quantity Code Number 1 11 0002 93 50 sheets 80 6117 81 For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To Ettan Spot Picker 18 1145 28 page 279 Picker Head 1 4mm for 1 mm thick gels 18 1147 03 page 279 Picker Head 1 4 mm for 1 5 mm thick gels 18 1147 05 page 279 Picker Head 2 0 mm for 1 mm thick gels 18 1143 64 page 279 Picker Head 2 0 mm for 1 5 mm thick gels 18 1143 37 page 279 Reference Markers 18 1143 34 page 279 GE Healthcare 2 D Electrophoresis Ettan Digester 2 G 4 is m K Z mSS AA U Ettan Digester enables robust in gel digestion of proteins prior to MALDI analysis e Forin gel digestion of proteins from 2 D electrophoresis gel plugs e Improved design allows robust and reproducible digestion sensitive down to the 100 fmol range e Special needle design minimizes plug loss e Preprogrammed optimized protocols e No need for C18 cleanup salt level controlled by preinstalled methods e Processing of up to 384 4 x 96 samples simultaneously in less than 8 h Ettan Digester is a highly versatile instrument designed to perform in gel digestion of proteins captured in 2 D gel electrophoresis spots The instrument acts directly on Ettan Spot Picker output files automatically tracking samples from spot picking to completed digestion using simple plug and play routines Default digestion protocols ar
51. Electrophoresis 2003 Dr Lei Wang Expansion of the genetic code Proteins the molecules that carry out most of the functions required for life are built from 20 genetically encoded amino acids In his grand prize winning essay Dr Wang describes how he engineered the bacteria Escherichia coli to make unnatural amino acids The biosynthesis strategy mimics the way that the common 20 amino acids are encoded These additional building blocks allow the design of proteins with new properties Dr Wang received his bachelor s degree in organic chemistry in 1994 and his master s degree in physical chemistry in 1997 At Peking University he did research in Dr Zhongfan Liu s laboratory where he used scanning probe microscopy to investigate the properties of nanoparticles He went to the United States to pursue graduate studies at the University of California at Berkeley Under the guidance of Dr Peter G Schultz Dr Wang developed a general method for genetically encoding unnatural amino acids into proteins in live cells GE amp Science Prize for Young Life Scientists is supported by GE Healthcare and the journal Science which is published by AAAS the nonprofit society Used with permission of AAAS 2007 Further information on how to enter plus past winners and their essays can be found at www gelifesciences com science Chapter 8 2 D Electrophoresis 259 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE 262
52. First Dimension 269 NEW IPGBox and IPG Kit 271 Second Dimension 274 Automated Gel Handling 279 SDS PAGE IEF 283 Mini Format Vertical Units 284 Standard Format Vertical Units 287 Related Vertical Equipment 290 Standard Flatbed Units 291 Automated Flatbed Units 298 Nucleic Acid Electrophoresis 300 Standard Format Submarine Units 301 Pulsed Field Gel Electrophoresis 302 Power Supplies 303 Electrophoresis Standards and Reagents 306 IEF SDS PAGE amp 2 D Electrophoresis 314 Immiobiline for IEF and Carrier Ampholytes 314 Protein Molecular Weight Markers and pl Markers 315 IMPROVED Rainbow Molecular Weight Markers 316 DNA Molecular Weight Markers 320 made2measure Products in this chapter can be customized to your precise requirements including small scale packs concentrations special blends specific QC testing and scale up In addition we offer a Room Temperature Stable reagent development and manufacturing service where we develop your 20 C reagents and kits for storage and shipping at room temperature Our manufacturing standards are certified to ISO 9001 2000 with Six Sigma implementation throughout the manufacturing process Contact us for more information at made2measure ge com and visit us on the web at www gelifesciences com custom To ensure that we handle your inquiry efficiently please provide the product name catalog code and volume requirements Supported by All instruments in this chapter are CPF E
53. For pricing information visit www gelifesciences com orderonline Mini Vertical Gel Casters e Cast up to 10 gels at once e Use with SE 250 SE 260 and miniVE Vertical Units ORDERING INFORMATION Product Quantity Code Number Gel Casters for 1 or 2 gels 10 x 10 5 cm plates Dual Gel Caster 1 80 6146 50 for 2 4 gels 10 x 10 5 cm plates 4 Gel Caster Complete 1 80 6146 12 for 2 4 gels 10 x 8 cm plates Mighty Small 4 Gel Caster Complete for SE 250 1 80 6151 06 Includes 5 rectangular glass plates 4 notched alumina plates 100 sheets wax paper space saver plate 5 filler sheets and set of filler plugs for 4 10 gels 10 x 8 cm plates Mighty Small Multiple Gel Caster Complete 1 80 6142 51 Includes 10 notched alumina plates 20 rectangular glass plates 2 red clamps 100 sheets wax paper space saver plate 5 filler sheets set of filler plugs and Spacer Mate assembly template Order combs and spacers separately For pricing information visit www gelifesciences com orderonline Order combs and spacers separately For more information visit www gelifesciences com ORDERING INFORMATION Wells No Thickness Width Vol of Quantity Code Number mm mm sample per 1 mm depth ul Teflon combs 5 0 75 13 0 95 1 80 6140 23 5 1 00 13 0 12 7 1 80 6140 42 5 150 13 0 1941 1 80 6140 61 9 1 00 S 5 8 1 80 6140 80 10 0 75 4 8 3 6 1 80 6138 71 10 1 00 4 8 4 8 1 80 6138 90 10 150
54. G INFORMATION Product Quantity Code Number Ettan DALTsix Electrophoresis Unit 115 V 1 80 6485 08 Includes Two pieces vinyl tubing two complete male female Quickfit connectors and cassette removal tool Ettan DALTsix Electrophoresis Unit 220 V 1 80 6485 27 Includes Two pieces vinyl tubing two complete male female Quickfit connectors and cassette removal tool Ettan DALTsix Upper Buffer Chamber NEW 1 DALT Precast Gel Cassette 1 80 6466 65 DALT Gel 12 5 26 x 20 cm 6 17 6002 36 DALT Buffer Kit for 12 precast gels 1 17 6002 50 1 1 1 28 9057 86 DALT Gel Casting Cassette 1 0 mm 80 6466 84 DALT Gel Casting Cassette 1 5 mm 80 6488 69 Low fluorescence Glass Plates 27 x 21 cm for Ettan DALT 80 6475 58 including spacers low fluorescence glass for use with fluorescent dues DALT Glass Plate Set including spacers 1 80 6475 39 regular glass plates for spot picking DALT Blank Cassette Insert 1 80 6467 03 DALT Separator Sheets 0 5 mm 16 80 6467 41 DALT Filler Sheets 1 0 mm 6 80 6467 60 DALTsix Gel Caster 1 80 6485 46 Includes 7 Separator Sheets 0 5 mm and 6 Filler Sheets 1 0 mm Order cassettes separately DALTsix Gradient Maker 1 80 6487 36 Equilibration Tube Set 12 80 6467 79 DALT Cassette Removal Tool 2 80 6474 82 DALT Cassette Rack 2 80 6467 98 Staining Tray Set 1 80 6468 17 Wonder Wedge plate separation tool 1 80 6127 88 Roller 1 80 1106 79 For pricing information visit www gelifesciences co
55. ICAL SPECIFICATIONS Nucleic Acid Electrophoresis Units Safety Certifications CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 UL3101 1 CSA22 2 1010 1 Supported by Crew Accessories for Gene Navigator Pulsed Field System Hexagonal Electrode Kit 1kit 18 1019 20 Includes Hexagonal electrode gel support tray 15 x 15 cm gel casting frame 15 x15 cm 1 mm thick 12 or 23 well comb 2 mm thick 12 or 23 well comb 2 mm thick preparative comb making 1 or 2 wells Point Electrode Kit 1kit 18 1019 23 Includes 6 cathode electrodes 2 anode electrodes gel support tray 20 x 20 cm gel casting tray 20 x 20 cm 2mm thick 22 well comb 302 For more information visit www gelifesciences com GE Healthcare Power Supplies EPS Power Supplies TECHNICAL SPECIFICATIONS Sqfetu certificotions EPS Power Supplies cover the wide range of electrophoresis and blotting applications Designed for performance EPS Power Supplies can be relied on for safety and reproducibility All EPS Power Supplies have the following safety certifications CE 89 336 EEC EMC directive All EPS power supplies offer CE 73 23 EEC LV directive e Easy to read digital display Timed or continuous runs with end of run alarm if desired Automatic parameter limit crossover to prevent overheating and protect experiments and equipment Automatic recovery after power failure Membrane keypads for easy programming Tw
56. Molecular Weight Markers IMPROVED e Monitor progress of protein electrophoresis and assess transfer efficiency and molecular weight of blotted proteins without staining e High intensity prestained multicolored bands allow distinction of closely related proteins e Full Range Rainbow Markers M 12000 to 225000 use ten separate proteins with six different colors e High Range Rainbow Markers M 12000 to 225000 use eight separate proteins with six different colors e Low Range Rainbow Markers M 3500 to 38000 use seven separate proteins with five different colors e For use on SDS PAGE Rainbow Molecular Weight Markers have been enhanced to enable faster and simpler identification of proteins on SDS polyacrylamide gels The ready to load markers provide sharper more intense bands on gels and blots and have improved band spacing for more accurate molecular weight determination The bright distinctive colors of the markers allow easier confirmation of transfer to blotting membranes and orientation Rainbow Molecular Weight Markers comprise a mixture of individually colored proteins that are combined to produce bands of equal color intensity The approximate protein concentration is 1 to 2 mg ml depending on the product Each 250 ul pack size is sufficient for use with 50 minigels 10 x 8 cm or 25 large gels 20 x 20 cm For more information visit www gelifesciences com ORDERING INFORMATION Product Quantity Code Number
57. N i 14C Protein MW Markers PAGE 319 i Persulfate ie s ee ol Markers IEF 319 See Carbamuylyte Calibration Kit IEF 2 D IEF 319 op Buffers DNA Molecular Weight Markers pole mele PAGE UU f on 50 Base Pair Ladder Agarose PAGE 320 DeStreak Reagents IEF rehydration solution 307 100 Bose Pair Ladder Agarose PAGE 321 EDTA Disodium Sqlt PAGE Sequencino 310 s BNA Parkar Agarose al Glycine PAGE 311 ieee Digests Agarose 321 usOne Reagents PlusOne Additives and Sample Treatment Ursa 9 IEF PAGE Sequencin 190 Formomide PAGE Sequencing 310 ABS ce SAGE isi Mercaptoethanol IEF PAGE 311 See ee SAGE re riton X Glycerol 87 IEF PAGE Sequencing 310 Tris PAGE Sequencing 190 PlusOne Detergents Sodium Dodeculsulfate PAGE 190 a ee La ee Dithiothreitol IEF PAGE 190 PlusOne Stains and Staining Kits Bromophenol Blue IEF PAGE Sequencing 190 Coomassie Blue R Tablets PAGE IEF 369 iodoacetamide IEF PAGE 311 DNA Silver Staining Kit PAGE 335 Thiourea IEF 2 D PAGE 312 Silver Staining Kit Protein IEF PAGE 368 Related Products Refer To ImageQuant Imagers page 384 For more information visit www gelifesciences com GE Healthcare Electrophoresis Standards and Reagents DeStreak Rehydration Solution DeStreak Rehydration Solution contains DeStreak Reagent which reduces nonspecific oxidation of proteins and streaking in 2 D gels and improves reproducibility significantly e Improves reproducibility and quality o
58. Refer To Ettan DIGE Imager 63 0056 42 page 383 DeCyder 2 D Differential Analysis Software v6 5 page 265 DeCyder Extended Data Analysis EDA Software v1 0 page 266 ImageMaster 2D Platinum v6 0 page 268 Ettan DALTtwelve Large Vertical System page 277 Ettan DALTsix Large Vertical System page 274 Multiphor II Electrophoresis System page 291 Ettan IPGphor 3 Isoelectric Focusing Unit 11 0033 64 page 272 Deep Purple Total Protein Stain page 367 The NHS ester reactive group of CyDye DIGE Fluor minimal dyes covalently attaches to the epsilon amino group of lysine of proteins via an amide linkage The ratio of dye to protein has been specifically designed to ensure the dyes are limiting in the reaction As a result approximately 3 of the available proteins are labeled and then only ona single lysine per protein i e one dye per protein or minimal labeling The amino acid lysine in proteins carries an intrinsic single positive charge at neutral or acidic pH CyDye DIGE Fluor minimal dyes also carry a single positive charge which when coupled to the lysine replaces the single positive charge of the lysine with its own ensuring that the pl of the protein does not significantly alter compared with the same unlabeled protein To achieve maximal labeling of cysteine residues the protocol uses a high fluor to protein labeling ratio This type of labeling method labels all available cysteines on each protein under the conditions used resulting in
59. W supported by Labcrew your GE Healthcare equipment professionals We provide Service Agreements and Preventive Maintenance to keep your instrument running in peak condition Additional services include equipment de installation re installation and software hardware upgrades Visit www delifesciences com labcrew for more information NOLLWATIVAS S NOLLVLNIWNDOG J 1SsD jAJ DBDuu e J p SO d e 1LOl 1DA31JOS III JQUUDDSeHDW e yuonO Dpulil e 1J Dpuul 39Id UD113 e LUJO1S e uooud e OT 4 1dpu2 s sw s s bulbow NOILYN TIVAJ S NOILLVLNAWNDOG NOILOJ3L3Q 6 1 dpy9 s 6Bun1ojg 6uijpupu jab p 1puuo1nv e S Dp SUID1S e 6 191dpu2 s Buluip1s NO 123130 sis 1oudo1J12 3 SISSYOHdOYLDI 14 x66 JH SS JH esouDby e q OT 0 dq COT winipa 9 1 d0y9 aas JOud u o BPILUD ALDDA Od e dq 00001 gt IIpuus TVOILATVNV plod 3 J9nN uj 1s s spud p 1Duio1nv e lI 4oudn ni J Puopudjs e p q1bl3 007 JS 099 3S Aqny 009 JS pippuD1S e 092 JS 0SZ JS FAIUIW IUIN o 091 43A 33 39Yd SAS AJ3WQ vag UONI IS WG UOJ 26107 e Aqny 009 3S pJopunis e FAIUILU IUIW UOISUSWWIP puo5 s sj 6 diu1sS iq ul iqouuul e 1oudodl U01133 e uolsu uiip 1S1 3 a z 1oudodl UD e anjam vq UONI xIST1VG UD e 3910 g 2 ul 9101d sloadOHdOdl ala S NI 13qv OT 4 1dpuo s Bui qp DIDD
60. al design The major source of error in a 2 D experiment is gel to gel variation To eliminate this an internal standard is run on all gels within an experiment Multiplexing using Ettan DIGE system is the only easy way in which an internal standard can be used Labeling and internal standardization for real 2 D differences Using Ettan DIGE system up to three protein samples labeled with different CyDye DIGE fluors can be run on the same 2 D gel simultaneously One of these the internal standard results from the pooling of aliquots of all biological samples in the experiment Two sets of dyes are available the minimal dyes and the saturation dyes from the scarce sample labeling kit With the minimal dyes the internal standard is labeled with one CyDye DIGE Fluor minimal dye e g Cy2 and is run together with individual samples labeled with other CyDye DIGE Fluor minimal dyes e g Cy3 and Cy5 With the saturation dyes from the CyDye DIGE Fluor Labeling Kit for Scarce Samples the internal standard is labeled with one dye e g Cy3 and is run together with individual samples labeled with the Cy5 due This means that every protein from all samples will be represented in the internal standard and each protein can therefore be compared to itself within the internal standard to generate a ratio of relative abundance The same internal standard is run on all gels within an experimental series thereby creating an intrinsic link across all gel
61. arations The system increases productivity while delivering high throughput resolution and reproducibility When used in combination with 24 cm Immobiline DryStrip gels Ettan DALTtwelve maximizes the number of discrete quantifiable protein spots obtainable per large format gel Ettan DALTtwelve System comprises a 12 gel separation unit programmable power supply control unit precast large format gels hinged gel cassettes and 14 gel capacity gel caster The separation unit requires only 10 I of buffer which is circulated by a pump located in the lower chassis of the unit The Peltier cooling unit at the bottom of the tank precisely controls the temperature over a range of 15 C to 50 C Up to 12 gels can be run simultaneously with separations complete in 4 6 h DALT Gel 12 5 26 x 20 cm and DALT Buffer Kit use a proprietary buffer formulation DALT Gel 12 5 and Buffer Kit for long shelf life and high reproducibility DALT Gel 12 5 26 x 20 cm is a precast polyacrylamide gel bound to a plastic support film which provides ease of handling and dimensional stability The gels are barcoded for convenient sample and batch tracking DALT Gel 12 5 is used together with DALT Buffer Kit which comprises concentrated buffers for running the gel Gel Buffer for inserting the gel into DALT Precast Gel Cassette and Sealing Solution for application of the IPG strip The buffer in the gel is part of a unique buffer system that gives longer shelf
62. are Standard Flatbed Units Dice approved ORDERING INFORMATION Product Quantity Code Number Multiphor II 1 18 1018 06 Includes Buffer tank 1 18 1122 25 Leveling feet 4 18 1026 40 Safety lid with high voltage leads 1 18 1122 26 Cooling plate ceramic 210 x 270 mm 1 18 1103 46 Grommets 2 80 1106 58 Hose clamps 10 18 1104 27 Cooling tubing 8 mm i d 12 mm o d 4m 80 1106 56 Tubing connector set female and male 4 18 1104 26 Electrode holder 1 80 1106 55 EPH IEF electrode anode 1 18 1121 53 EPH IEF electrode cathode 1 18 1121 52 For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To EPS 3501 XL Power Supply 18 1130 05 page 305 MultiTemp III Thermostatic Circulator page 276 Processor Plus page 370 TECHNICAL SPECIFICATIONS Safety certifications CE 89 336 EEC EMC directive CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 UL3101 1 CSA22 2 1010 1 A Buffer tank B Leveling feet C Safety lid with high voltage leads D Cooling plate ceramic li 210 x 270 mm N a 1 E Grommets l t J F Hose clomps a G Cooling tubing 8 mm i d 12 mm o d H Tubing connector female and male I Electrode holder J EPH IEF electrode anode K EPH IEF electrode cathode For more information visit www gelifesciences com udo1125 3 SIS 1O 291 n w O O O jad Q LLJ 292 SDS PAGE IEF Standard F
63. astGel Sample Applicators 8 x 1 ul 50 18 1618 01 PhastGel Sample Applicators 6 x 4 pl 50 18 0012 29 Sample well stamp 1 18 0097 01 PhastTransfer Kit 1 18 1001 23 For pricing information visit www gelifesciences com orderonline Related Products PhastGel Refer To page 299 The stainless steel development unit is capable of processing two gels simultaneously at temperatures from ambient to 50 C Up to nine different reagents can be used ina single processing program The combination of PhastSystem with PhastGels and buffer strips provides an integrated system of unsurpassed quality flexibility and reproducibility with a minimum of manual involvement A substantial list of applications testifies to the utility of PhastSystem Visit www gelifesciences com to access application notes and a reference list on the use of PhastSystem TECHNICAL SPECIFICATIONS Development Unit Development Programmable parameters Development temp range Dimensions W x D x H Weight Safety certifications Electrode cassette Electrodes Chemical resistance Dimensions W x D x H Gel backing remover Capacity 1 or 2 gels Volume 80 ml solution Number of ports 9 plus 1 for waste Agitation Rotation Solutions automatically pumped Inlet port outlet port duration temperature and an alarm for 9 programs of 20 steps Ambient to 50 C 30 x 50 x 14 cm 4 8 kg CE 89 336 EEC EMC directive CE 73 23 EEC LV directive
64. ate pH 5 6 6 6 3 80 1128 32 Accessories Immobiline sample application strips 52 samples 5 20 ul 5 18 1002 76 Sample application pieces 200 80 1129 46 For pricing information visit www gelifesciences com orderonline TECHNICAL SPECIFICATIONS Gel dimensions after rehydration 245 x 110 x 0 5 mm W x L x T Gel composition T C 4 3 Storage temperature C 20 GE Healthcare SDS PAGE IEF Standard Flatbed Units Multiphor Il and ExcelGel System for second dimension e Perform second dimension separations using convenient precast gels and 7 11 18 or 24 cm IPG strips P e Choose from ExcelGel precast SDS polyacrylamide gels Imm available as 12 5 homogeneous and 12 14 gradient gels Exce e Prepare multiple 2 D maps on a single gel using three 7 cm or two 11 cm IPG strips side by side e Configure Multiphor II with MultiTemp III Thermostatic Circulator and the programmable EPS 1001 Power Supply for reproducible running conditions M utfer Strin Docitinner camnilete Mu 10 uTrer Strip rositioner compiete For pricing information visit www gelifesciences com orderonline i acs Related Products Refer To The new Multiphor Il Buffer Strip Positioner facilitates placement Malitrenapilthemnoctaite Cieuletor ERETT of ExcelGel Buffer Strips and holds the strips securely in place EPS 1001 Power Supply page 304 during the run For additional information on Multiphor Il ExcelGel precast g
65. cale are nonlinear to distribute the Immobiline DryStrip pH 3 10 NL 18 cm 12 17 1235 01 proteins evenly over the gel length to obtain maximal Immobiline DryStrip pH 3 10 18 cm 12 17 1234 01 resolution Immobiline DryStrip gels 13 cm o Immobiline DruStrip pH 3 5 6 NL 13 cm 12 17 6003 55 Immobiline DruStrip gels are rehydrated with a matching IPG Immobiline DryStrip pH 5 3 6 5 13 cm io E Buffer prior to use Samples can be included directly in the Immobiline DryStrip pH 6 2 7 5 13 cm 12 17 6003 65 rehydration solution for efficient loading of dilute samples or for Immobiline DryStrip pH 7 11 NL 13 cm 12 17 6003 70 loading larger amounts of protein Samples can also be loaded Immobiline DryStrip pH 3 11 NL 13 cm 12 17 6003 75 after rehydration The first dimension separation is carried out Immobiline DryStrip pH 4 7 13 cm 12 17 6001 13 using Ettan IPGphor 3 IEF system or Multiphor II flatbed system Immobiline DryStrip pH 6 11 13 cm 12 17 6001 96 i _ Immobiline DruStrip pH 3 10 NL 13 cm 12 17 6001 15 Immobiline DruStrip pH 3 10 13 cm 12 17 6001 14 WER Immobiline DryStrip gels 11 cm a Immobiline DryStrip pH 3 5 6 NL 11 cm 12 17 6003 54 sa gt Immobiline DryStrip pH 5 3 6 5 11 cm 12 17 6003 59 3 i4 e Immobiline DryStrip pH 6 2 7 5 11 cm 12 17 6003 64 ia Immobiline DryStrip pH 7 11 NL 11 cm 12 17 6003 69 o Immobiline DryStrip pH 3 11 NL 11 cm 12 17 6003 74 i Immobiline DryStrip pH 4 7 11 cm 12 18 1016 60 nt Immob
66. can also be used for flatbed second dimension separations see page 291 Five choices for second dimension SDS PAGE Choose from five formats for performing second dimension SDS PAGE depending on your throughput and resolution requirements For large format 26 x 20 cm separations with high throughput resolution and reproducibility choose Ettan DALTtwelve see page 277 or Ettan DALTsix Large Vertical Systems see page 274 When used in combination with 24 cm Immobiline DryStrip gels Ettan DALT systems maximize the number of discrete quantifiable protein spots obtainable per large format gel DALT Gel 12 5 Buffer Kit and Precast Gel Cassettes deliver reproducible results with high throughput and convenience Typical separation time is 4 5 h for six gels GE Healthcare The combination of Multiphor II Flatbed Electrophoresis Unit and ExcelGel precast SDS acrylamide gels see page 297 offers q convenient and reproducible method for performing second dimension separations IPG strips up to 24 cm can be used with ExcelGel SDS gels For comparative studies three 7 cm or two 11 cm IPG strips can be run side by side on one gel Separation time is less than 4 h SE 600 Ruby Vertical Unit see page 287 enables the analysis of several samples under identical conditions The SE 600 can use 7 or 13 cm IPG strips When using two 7 cm IPG strips side by side up to eight second dimension separations can be performed at once because t
67. cast polyacrylamide gels make a wide range of ExcelGel SDS and Ampholine PAGplate products are supplied electrophoretic techniques readily available with minimum fully hydrated and ready to use CleanGel Immobiline DryPlate preparation The manufacturing quality and reproducibility of and DryStrip products are supplied dry and must be rehydrated these gels translate into highly reliable and reproducible results in the appropriate buffer before use Prepared swelling solutions with minimal training are available for the most common procedures All precast gel recipes are proprietary However buffer formulations for a variety of other applications are included in the instructions SELECTION GUIDE BY TECHNICAL OVERVIEW Precast Gels and Buffer Kits for Multiphor II System Application Sample Sample Protein DNA Product Application No Gel Vol ul Buffer Quantity Page Ei m ExcelGel SDS Grodient 8 18 strip 26 1 40 A 6 295 VN ExcelGel SDS Homogeneous ET m m 7 5 well 25 5 17 A 6 295 q m m 12 5 well 25 5 17 A 6 295 E E 15 well 25 5 17 A 6 295 O El Ei ExcelGel SDS Buffer Strips anode and cathode C D 6 pairs 295 Ampholine PAGplate IEF El pH 3 5 9 5t strip 26 52 1 40 5 O a pH 4 0 6 5t strip 26 52 1 40 5 mi pH 4 0 5 0t strip 26 52 1 40 5 UO m CleanGel IEF strip 26 52 1 40 5 296 K Immobiline DryPlate IEF LL m pH 4 0 7 0 strip 26 52 1 40 3 296 E pH 4 2 4 9 strip 26 52 1 40 3 296 m pH 4 5 5 4 strip 26 52 1
68. collaboration with GeneBio and GE Healthcare It is powered by the innovative and widely used Melanie gel analysis software The flexible and intuitive nature of ImageMaster 2D Platinum v6 0 allows users to perform analyses specific to their individual requirements For more information visit www gelifesciences com DIGE approved ORDERING INFORMATION Product Quantity Code Number Image Master 2D Platinum 7 0 1 license NEW 1 28 9380 91 Image Master 2D Platinum 7 0 DIGE 1 license NEW 1 28 9380 55 Image Master 2D Platinum 7 0 upgrade to DIGE 1 1 28 9398 10 license NEW Image Master 2D Platinum 6 0 upgrade to 7 0 1 1 28 9399 70 license NEW Image Master 2D Platinum 6 0 DIGEtupgrade to 7 0 DIGE 1 28 9380 80 1 license NEW For pricing information visit www gelifesciences com orderonline Other packages available Please contact your sales representative Note An e license is required for access to ImageMaster 2D Platinum v7 0 Related Products Code Number Refer To ImageScanner III 28 9076 07 page 387 Ettan IPGphor 3 Isoelectric Focusing Unit 11 0033 64 page 272 Ettan DIGE Imager 63 0056 42 page 383 Typhoon 9210 63 0055 76 page 376 Ettan Spot Picker 18 1145 28 page 279 Workspace project folders Operation ua Gels Detecting Matching Classes Analyzing Ld Reports Exporting Documents Image analysis from start to finish in the workspace GE Healthcare 2 D Electrophoresis First Dimension
69. d by reductive methylation to decrease autolysis of the enzyme and increase stability The contents of each vial are reconstituted with 6 5 ml of 20 mM ammonium bicarbonate and then diluted with a further 6 ml of ammonium bicarbonate before use For more information visit www gelifesciences com udo112 3 O x OD T 313 n w O O O Q LL 514 IEF SDS PAGE amp 2 D Electrophoresis Immobiline for IEF and Carrier Ampholytes Carrier Ampholytes and Immobiline for IEF e Linear stable pH gradients with even conductivity across the gradient e High buffering capacity e Reproducible pl determination e Broad and narrow pH ranges available Immobiline Il Reagents Immobiline II Reagents are buffering acrylamide derivatives with different dissociation constants When different pK values of Immobiline are mixed in appropriate proportions in a gradient maker together with acrylamide bisacrylamide TEMED and ammonium persulfate a pH gradient is formed that is covalently attached to the polyacrylamide gel Immobiline II reagents can form any narrow or wide pH gradient in the 3 8 10 range without gradient drift and conductivity gaps Because the pH gradient is completely immobilized and stable reproducible linear gradients with a slope as low as 0 01 pH units cm can separate proteins with pl differences of 0 001 pH units Pharmalyte Pharmalyte carrier ampholytes prepared by t
70. de 1 80 1257 87 Electrode paper NovaBlot 200 x 250 mm 500 80 1106 19 Cellophane sheets 210 x 320 mm 50 80 1129 38 For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product Quantity Code Number Preserving Cellophane sheets 210 x 320 mm 50 80 1129 38 Gel Support GelBond PAGfilm 124 x 258 mm 50 80 1129 36 GelBond PAGfilm 203 x 260 mm 50 80 1129 37 GelBond film agarose 84 x 94 mm 50 80 1129 33 GelBond film agarose 124 x 258 mm 50 80 1129 32 Electrodes Paper Electrode Strip and Wicks IEF electrode strips 100 18 1004 40 EPH electrode anode 1 18 1122 20 EPH electrode cathode 1 18 1122 19 EPH electrode wicks 82 x 130 mm 500 80 1129 53 EPH electrode wicks paper 104 x 253 mm 500 80 1129 52 Electrode paper NovaBlot 200 x 250 mm 500 80 1106 19 Sample Application SDS sample application strips 26 samples 40 ul 5 18 1002 74 IEF SDS sample application strips 52 samples 5 20 pl 5 18 1002 26 Immobiline sample application strips 52 samples 5 20 ul 5 18 1002 76 Sample application pieces 200 80 1129 46 EPH IEF sample application foil agarose 24 samples 5 80 1129 47 2 4 ul Sample cups Immobiline DryStrip Kit 60 18 1004 35 For more information visit www gelifesciences com For pricing information visit www gelifesciences com orderonline udo1125 3 O k OD T 293 SDS PAGE IEF Standard Flatbed Units Introduction to Multiphor II Precast Gels Pre
71. dissolved in octamethylcyclooctasilane a low toxicity environmentally safe solvent It is used to inhibit sticking of polyacrylamide gels agarose gels and nucleic acids to glass surfaces Sodium Dodecylsulfate SDS SDS is an ionic detergent used to solubilize proteins The PlusOne SDS reagent is carefully controlled for carbon chain length and overall quality PlusOne TEMED TEMED is a catalyst for the polymerization of acrylamide when used with ammonium persulfate TECHNICAL SPECIFICATIONS M 116 21 Minimum assay 99 Thiourea Thiourea is a chaotrope that is used together with urea in the IPG strip rehydration solution to solubilize and denature proteins unfolding them to expose internal ionizable amino acids Thiourea increases the solubility of membrane proteins Tris Qualified for 2 D electrophoresis Tris tris hydroxymethul aminoethane is suitable for preparing electrophoresis buffer in the pH range 7 2 to 9 0 Triton X 100 A nonionic or zwitterionic detergent is included in the sample solution to ensure complete sample solubilization and to prevent aggregation through hydrophobic interactions Triton X 100 is a nonionic detergent commonly used in IEF PlusOne Tween 20 Tween 20 polyoxyethylene 20 sorbitan monolaurate is a mild detergent for solubilization of native proteins It can be used in polyacrylamide and agarose gels for electrophoresis and IEF Density of Tween 20 at 20 C is 1 1 g ml Fo
72. e broad range pH 3 10 25ml 17 0456 01 Pharmalyte narrow range pH 4 2 4 9 25ml 17 0562 01 Pharmalyte narrow range pH 4 5 5 4 25ml 17 0563 01 Pharmalyte narrow range pH 5 6 25ml 17 0564 01 Pharmalyte narrow range pH 6 7 7 7 25ml 17 0566 01 For pricing information visit www gelifesciences com orderonline Store at 4 C to 8 C For new products in this area please inquire at your local sales office GE Healthcare IEF SDS PAGE amp 2 D Electrophoresis Protein Molecular Weight Markers and pl Markers Protein and Peptide Molecular Weight Markers LMW HMW SDS HMW Native Product Quantity Code Number 97 0 50 Peptide Marker Kit 1 vial 2 mg vial 80 1129 83 66 0 LMW SDS Marker Kit 10 vials 575 ug vial 17 0446 01 45 0 116 669 HMW SDS Marker Kit 10 vials 175 ug vial 17 0615 01 30 0 76 440 HMW Native Marker Kit 10 vials 250 ug vial 17 0445 01 232 For pricing information visit www gelifesciences com orderonline FF _ 53 0 140 Unopened vials should be stored at 4 C Related Products Refer To miniVE Verticol Electrophoresis Sustem page 283 66 SE 600 Ruby and SE 660 Dual Cooled Vertical Units page 287 e Determine molecular weight of uncharacterized protein samples with precision by comparison with carefully m selected standards O e Get clear sharp bands for easy measurement of migration O LMW A 3 ul aliquot of a two fold dilution of LMW Marker Kit was separated on a dis
73. e O S a Dg DALTsix Gel Caster for casting 1 0 and 1 5 mm large format gels and DALTsix Gradient Maker OD N N a P Exploded view of Ettan DALTsix Electrophoresis Unit a lid with leads and safety interlock b upper buffer chamber c gel cassette d anode assembly cassette carrier e lower buffer chamber with ceramic heat exchanger and buffer circulation pump GE Healthcare For more information visit www gelifesciences com 275 n w O O O Q LL 276 2 D Electrophoresis Second Dimension MultiTemp III Thermostatic Circulator MultiTemp III is a compact and reliable temperature control unit for electrophoresis equipment The connection to Multiphor II cooling plate is shown above Controls coolant temperature from 10 C to 90 C 0 1 C Coolant circulates at 12 l min with internal pump CFC free refrigerant protects the environment MultiTemp III refrigerated bath circulator provides reliable and reproducible temperature control by circulating constant temperature liquid to external electrophoresis equipment The working temperature range spans requirements from heat sensitive enzyme separations to highly denaturing DNA sequencing The generous 200 W cooling capacity handles two Multiphor II units An opening in the reservoir top allows MultiTemp III to be used as a small high precision constant temperature water bath for bottles and tubes MultiTemp Ill op
74. e from narrow 1 pH unit and medium 3 pH units pH ranges e Handle mechanically stable plastic backed gels with confidence Immobiline DryPlate precast gels contain preformed immobilized non drifting pH gradients The gels are rehydrated to a thickness of 0 5 mm in the reswelling cassette accessory before use Immobiline technology produces an absolutely stable pH gradient allowing extremely high voltages and high resolution over narrow pH ranges even for low mobility proteins that require long focusing times For more information visit www gelifesciences com ORDERING INFORMATION Product Quantity Code Number CleanGel IEF 5 18 1035 32 Accessories CleanGel IEF electrode strips 12 18 1035 33 GelPool 1 18 1031 58 Sample application pieces 200 80 1129 46 IEF SDS sample application strips 52 samples 5 20 ul 5 18 1002 26 SDS sample application strips 26 samples 40 ul 5 18 1002 74 For pricing information visit www gelifesciences com orderonline Refer To page 314 Related Products Pharmalyte TECHNICAL SPECIFICATIONS Gel dimensions after rehydration 250 x 110 x 0 5 W x L x T mm Gel composition T C 5 3 Storage temperature C 20 ORDERING INFORMATION Product Quantity Code Number Immobiline DryPlate pH 4 0 7 0 3 80 1128 28 Immobiline DryPlate pH 4 2 4 9 3 80 1128 29 Immobiline DryPlate pH 4 5 5 4 3 80 1128 30 Immobiline DryPlate pH 5 0 6 0 3 80 1128 31 Immobiline DryPl
75. e improved easy to use Ettan IPGphor 3 system overcomes the most common hurdles of existing technology to consistently deliver speed reproducibility and handle increased protein loads Ettan IPGphor 3 unit has undergone a complete redesign to provide an even higher level of quality and reproducibility with improved sample handling and PC control Efan IMaphor T Se Lle L Jarti Fai Er Fun sabbzyga B dakad Sennen eg Strip ingia Bl range iam 47 4 i Four ol uripi Erg nrni defali i Instri Step 4 4 Mared Seipolri MOD V LOCK V TA p T paii hrat 31506 Vh 06 27 10 Emad Do go ma a do moo da gago Gie Gai G okoa oee tra Fieapared riage Y W ease oaren A Tatal time ee Graphical display of voltage current and volt hours For more information visit www gelifesciences com Supported by E approved ORDERING INFORMATION Product Quantity Code Number Ettan IPGphor 3 Isoelectric Focusing Unit 1 11 0033 64 Basic unit includes cable Ettan IPGphor 3 Control Software and Ettan IPGphor 3 Tutorial CD Cable Serial 9 Pin F F with Null Modem Adapter 1 80 6506 36 Ettan IPGphor 3 Control Software 1 11 0036 23 USB to RS232 Adapter 1 28 4001 45 Ettan IPGphor Manifold Ettan IPGphor Manifold Complete 1 80 6498 38 IPGphor Manifold ceramic tray 1 80 6498 57 Sample cups 120 80 6498 95 Paper electrode 240 80 6499 14 Paper bridge pads 120 80 6499 33 Electrode set 1 8
76. e optimized and compatible with Coomassie silver and fluorescence staining systems A method editor also allows customization of methods Standard microplates further increase ease of use as do low salt digestion buffers which eliminate the need for desalting Eight samples are handled in parallel and 384 samples can be processed in less than 8 h Ettan Digester is supplied with computer and monitor control software 500 ul syringe needles enzyme tube holder enzyme tubes calibration plate calibration tool tubing kit tubings and fittings and waste bottle A protective hood which minimizes risk of contamination is optional 100 fmol of phosphorylase B or BSA in an SDS gel plug could be identified by loading 10 of the resulting digested peptide mix onto the MALDI target GE Healthcare Automated Gel Handling Supported by OGE approved ORDERING INFORMATION Product Quantity Code Number Ettan Digester 1 18 1142 68 Including computer and monitor Accessories Enzyme container 48 1 18 1157 75 Enzyme tube 1000 18 1175 81 Enzyme tube holder 1 18 1175 82 Syringe 500 ul 1 18 1143 56 Needle Digester 2 18 1143 57 Tubing and fitting Digester 1 18 1143 62 Tubing kit 1 18 1152 64 Waste Bottle and Tubing 1 18 1143 44 Screw cap with Hole 1 18 1155 36 Calibration plate 1 18 1156 66 Calibration tool 1 18 1175 79 Sealing foil 100 18 1143 54 Protective Hood 1 18 1149 17 For pricing information visit w
77. e platform Ettan Spotter is supplied with computer and monitor control software 100 ul syringe needle vial with cap and tubings and fittings A protective hood which minimizes the risk of contamination is optional For more information visit www gelifesciences com Supported by OGE approved ORDERING INFORMATION Product Quantity Code Number Ettan Spotter 1 18 1142 67 Includes computer and monitor Accessories MS sample tray Bruker without target 1 18 1155 99 MS sample tray Micromass without target 1 18 1156 00 MS sample tray Voyager without target 1 18 1156 02 MS sample tray Shimadzu without target 1 11 0006 79 Target Layout Upgrade Software 1 11 0002 94 Vial with cap 1 ml 100 18 1152 75 Needle 1 18 1149 56 Tubing and Fittings 1 18 1156 30 Ettan Sample Slide Kit 2 18 1147 18 Syringe 100 ul 1 18 1143 55 Screw cap with Hole 1 18 1155 36 Waste Bottle and Tubing 1 18 1143 44 Protective Hood 1 18 1149 17 For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To Ettan Spot Picker 18 1145 28 page 279 Ettan Digester 18 1142 68 page 281 TECHNICAL SPECIFICATIONS Sample source Microplates with peptide or protein samples dried or in solution 384 samples batch mode 96 samples manual mode Depends on method used 96 samples in less than 45 min if Dissolve in matrix first is used 18 C to 30 C 65 F to 86 F 80 noncondens
78. ectrophoresis buffers to inhibit metal requiring nucleases PlusOne Formamide A mild denaturant for nucleic acids similar to urea formamide is used in denaturing nucleic acid electrophoresis and hybridization TECHNICAL SPECIFICATIONS M 45 04 Minimum assay 99 DNases exo and endonucleases Not detected RNases Not detected PlusOne Glycerol Glycerol is a stabilizing medium for casting polyacrylamide gradients In IEF the pH gradient can be stabilized by introducing 10 15 glycerol It also stabilizes polyacrylamide gels for preservation TECHNICAL SPECIFICATIONS M 92 09 Minimum assay 86 to 88 Density at 20 C 1 2 g ml For more information visit www gelifesciences com DIGE approved ORDERING INFORMATION Product Quantity Code Number Dithiothreitol 1g 17 1318 01 For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product PlusOne EDTA disodium salt Quantity Code Number 100g 17 1324 01 For pricing information visit www gelifesciences com orderonline Store at room temperature TECHNICAL SPECIFICATIONS M 372 24 Minimum assay 99 5 ORDERING INFORMATION Product PlusOne Formamide Quantity Code Number 250ml 17 1320 01 For pricing information visit www gelifesciences com orderonline Store dry at room temperature OGE approved Quantity Code Number 11 17 1325 01 ORDERING INFORMATION Product Plu
79. ee protocols Outstanding choice for non lEF ultrathin flatbed gels on Multiphor Il systems For more information visit www gelifesciences com ORDERING INFORMATION Product Quantity Code Number 1 18 1130 01 EPS 301 Power Supply For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To miniVE Vertical Electrophoresis System page 283 Nucleic Acid Electrophoresis page 300 ImageQuant Imagers page 384 ORDERING INFORMATION Product Quantity Code Number 1 18 1130 02 EPS 601 Power Supply For pricing information visit www gelifesciences com orderonline Related Products Refer To SE 600 Ruby and SE 660 Dual Cooled Vertical Units page 287 Nucleic Acid Electrophoresis page 300 ORDERING INFORMATION Product EPS 1001 Power Supply Quantity Code Number 1 18 1130 03 For pricing information visit www gelifesciences com orderonline GE Healthcare Power Supplies EPS 3501 XL e 3500 V 400 mA 200 W maximum e Constant voltage constant current constant power and linear voltage ramp modes e Linear voltage ramp mode brings proteins to focused equilibrium in IPG strips quickly without danger of overheating e Volt hour and milliampere hour modes compensate for variations in gel thickness sample ionic strength and temperature e Stores and recalls up to nine protocols each with nine phases and phase end alarms e Program automatic shut
80. el casting kit with casting tray and running tray 1 80 6053 78 7 x 10 cm Foam gasket 80 6053 97 Electrode replacement kit 1 80 6053 21 Wells Thickness Width No mm mm Quantity Code Number HE 33 Combs 1 21 1 0 44 6 1 80 6051 88 1 21 15 44 6 1 80 6052 07 8 1 0 6 5 1 80 6051 50 8 15 6 5 1 80 6051 69 12 ig 39 1 80 6050 74 12 15 3 9 1 80 6050 93 16 1 0 2 6 1 80 6051 12 16 15 2 6 1 80 6051 31 For pricing information visit www gelifesciences com orderonline Without back Order 80 6050 36 comb back separately t Preparative reference wells Related Products Code Number Refer To EPS 301 Power Supply 18 1130 01 page 304 Agaroses page 308 DNA Molecular Weight Markers page 320 TECHNICAL SPECIFICATIONS Nucleic Acid Electrophoresis Units CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 UL3101 1 CSA22 2 1010 1 Safety Certifications For more information visit www gelifesciences com udo112 3 O x D T 301 Nucleic Acid Electrophoresis Standard Format Submarine Units Pulsed Field Gel Electrophoresis HE 99X Max Submarine Unit Product Quantity Code Number HE 99X Max Submarine Unit Basic 1 80 6061 38 Order gel casting kit comb and comb back separately HE 99X Max Submarine Unit Complete 1 80 6061 57 i i Includes Basic unit 15 x20 cm gel casting kit one 1 5 mm thick 15 well comb and comb M lt back Boor or Accessories and Replace
81. electric focusing gel in the first dimension and separated by SDS PAGE in the second dimension Electrophoresis is simplified with Ettan IPGphor 3 or Multiphor II with Immobiline DryStrip gels in the first dimension and Ettan DALTtwelve or Ettan DALTsix electrophoresis systems in the second dimension The ability to multiplex different CyDye DIGE Fluor minimal dye labeled samples on the same gel means that the different samples will be subject to exactly the same first and second dimension running conditions Consequently the same protein labeled with any of the CyDye DIGE Fluor minimal dyes and separated on the same gel will migrate to the same position on the 2 D gel and overlay This limits experimental variation and ensures accurate within gel matching CyDye DIGE Fluor Labeling Kits for Scarce Samples Two labeling kits for scarce samples are available one contains the Cy3 and Cy5 saturation dyes the other contains those and an additional vial of Cy3 dye to label a preparative gel Each kit contains sufficient dye for at least 12 labeling reactions and allows labeling of as little as 5 ug protein per labeling reaction The CyDye DIGE Fluor Labeling Kit for Scarce Samples and Preparative Gel Labeling contains an additional vial of Cy3 dye for labeling up to 500 ug of protein The saturation dyes Cy3 and Cy5 from the Labeling Kit for Scarce Samples retain the advantages described for the minimal dyes The saturation dyes in the two kits
82. els m and accessories see page 294 OD x O Oo Three 2 D maps can be prepared on one gel gt g using Multiphor Il in conjunction with O Immobiline DruStrip 7 cm gels and ExcelGel 2 D Homogeneous 12 5 Request OD Application Note 80 6443 47 for details VN TECHNICAL SPECIFICATIONS ExcelGel Precast Gels N GE Healthcare For more information visit www gelifesciences com 297 T u O a O lt Q LLJ 298 SDS PAGE IEF Automated Flatbed Units PhastSystem PhastSystem provides automated protein and nucleic acid electrophoresis with ready to use gels buffer strips stain kits and proven protocols e Automatically separate and stain proteins under controlled power and temperature conditions e Program and store nine separation protocols of nine steps each and nine staining protocols of 20 steps each e Regulate gel temperature through Peltier controlled support bed e Assure reproducibility with quality tested precast gels buffer strips and staining kits PhastSystem consists of a Separation Control unit for system control and electrophoresis and a Development Unit for gel staining The optional PhastTransfer Kit quickly converts the system into a semi dry blotting unit Electrophoresis is performed on a thermostatic plate capable of maintaining temperatures accurately from 0 C to 70 C Multistep programming capability allows gels to be prerun loaded and run automatically Con
83. ence Difference Gel Electrophoresis 2 D DIGE ImageMaster 2D Platinum v6 0 e New DIGE enabled version uses the DeCyder 2 D co detection algorithm and supports the internal standard approach e Workspace feature combines all gel matching and analysis data and controls all operations e No arbitrary reference gel is necessary Compares gel populations directly thus adding significance to your analysis results e Updated display features such as multiple interlinked images tables and graphs allow you to interact with your results easily access all analytical data and work with a virtually unlimited number of gels e Dockable report windows let you customize the application layout according to your personal preferences e E licensing allows easy access to the software with added security l w a a n Merre Un Vous Ger Ga D3 Crj omi Gel Oo Cot Treated ik 1 far L doth ula wlej a a 2 n J ajel ImageMaster 2D Platinum v6 0 user interface with dockable windows simplifies the process of 2 D gel image analysis DIGE enabled version shown ImageMaster 2D Platinum v6 0 combines the superior spot detection and reliable matching of ImageMaster 2D Platinum v5 0 with the unique spot co detection of DeCyder 2 D Differential Analysis Software v6 5 Version 6 0 offers one common interface for both DIGE and non DIGE gels ImageMaster 2D Platinum v6 0 was developed by the Swiss Institute of Bioinformatics in
84. er 25ml 17 1330 01 ORDERING INFORMATION Product PlusOne Bind Silane For pricing information visit www gelifesciences com orderonline Store dark and dry at room temperature ORDERING INFORMATION Product Quantity Code Number PlusOne Boric Acid 500g 17 1322 01 For pricing information visit www gelifesciences com orderonline Store at room temperature TECHNICAL SPECIFICATIONS M 61 83 Minimum assay 99 8 ORDERING INFORMATION Product Quantity Code Number Bromophenol Blue 10g 17 1329 01 For pricing information visit www gelifesciences com orderonline DIGE approved ORDERING INFORMATION Product Quantity Code Number CHAPS 1g 17 1314 01 For pricing information visit www gelifesciences com orderonline For more information visit www gelifesciences com udo112 3 O x OD T 309 n oD a O Ma O O a Q O LLJ 310 Electrophoresis Standards and Reagents Dithiothreitol DTT Reducing agents are frequently included in the sample solution to break any disulfide bonds present and to maintain all proteins in their fully reduced state The most commonly used reductant is dithiothreitol DTT PlusOne EDTA disodium salt a t m mn plus EDTA sodium salt Cote Ne 17 1220601 r PlusOne EDTA disodium salt EDTA ethylenediaminetetraacetic acid disodium salt dihydrate a cation chelator is used in DNA el
85. erating parameters are easily set with membrane keys on the control panel The clear LED display shows set or actual temperature The stainless steel reservoir Is easy to clean and can be quickly drained through a convenient outlet at the front of the unit Supported by OGE approved Laba ORDERING INFORMATION Product Quantity Code Number MultiTemp lll Thermostatic Circulator 115 VAC MultiTemp lll Thermostatic Circulator 230 VAC Includes Insulation tubing for cooling and 3 way valve set 1 18 1102 77 1 18 1102 78 Accessories and Replacement Parts Cooling tubing 8 mm i d 12 mm o d 4m 80 1106 56 Tubing connector set female and male 4 18 1104 26 Insulation for cooling tubing 14 mm i d 27 mm o d 1 8m 80 1116 11 3 way valve set 1 18 1106 39 QuickFit Connector female 3 8 2 80 6115 15 QuickFit Connectors male 3 8 2 80 6115 53 Attached equipment cools quickly with 3 I reservoir capacity For pricing information visit www gelifesciences com orderonline TECHNICAL SPECIFICATIONS Working temperature range 10 C to 90 C Temperature control constancy lt 0 1 C at 20 C Range of temperature limit switch 20 C to 95 C Heater capacity 800 W Cooling capacity 115 VAC 240 W at 20 C 200 W at 5 C Cooling capacity 230 VAC 265 W at 20 C 215 W at 5 C Compressor coolant KLEA 134a Bath volume 3 Bath opening depth 13 x 10 10 cm Pump capacity pressure 0 32 bar Pump capacity flow rate
86. es from multiple LC MS experiments and is able to detect small differences between peptides with high statistical confidence Statistical analysis is carried out on all peptide peaks within minutes GE Healthcare ORDERING INFORMATION Product Quantity Code Number DeCyder MS 2 0 1 28 9078 84 including ONE additional concurrent networked e license DeCyder MS 2 0 1 28 9083 59 demo license no user manual amp low cost packaging including 8 week single node locked e license to be used for demo purposes only DeCyder MS 2 0 1 28 9083 60 single user concurrent networked e license DeCyder MS 2 0 1 28 9083 61 single user concurrent networked e license and including PC DeCyder MS 2 0 1 28 9083 62 FIVE additional concurrent networked e licenses DeCyder MS 2 0 1 28 9083 64 TEN additional concurrent networked e licenses DeCyder MS 2 0 1 28 9086 31 upgrade from 1 0 ONE concurrent networked e license For pricing information visit www gelifesciences com orderonline DeCyder MS Differential Analysis Software consists of two analysis features Peptide Detection with the PepDetect module and Run to Run Matching with the PepMatch module PepDetect module The detection module provides consistent and accurate peptide detection background subtraction isotope and charge state deconvolution as well as peak volume calculations using novel imaging algorithms The software s visualization tools offer the user ne
87. es on a single 2 D gel providing accurate quantitation reproducibility ease of use and increased throughput Reduced system variability enables accurate study of protein abundance differences against a baseline of inherent biological variation The system consists of three main components e Protein visualization CyDye DIGE fluors e Image capture Typhoon Variable Mode Imager or Ettan DIGE Imager e Image analysis DeCuder 2 D Differential Analysis Software or ImageMaster 2D Platinum DIGE Enabled software For more information visit www gelifesciences com Ettan DIGE system brings statistical confidence and reliability to 2 D electrophoresis Combining novel proprietary technologies in fluorescence multiplexing and image analysis Ettan DIGE system is a fully integrated system offering significant benefits over classical 2 D electrophoresis Ettan DIGE system uses a defined procedure that includes an internal standard to generate the most accurate results Using the system up to three protein samples are labeled with novel size and charge matched CyDye DIGE fluors and co separated on the same 2 D electrophoresis gel Imaging is performed using the Typhoon Variable Mode Imager or Ettan DIGE Imager Image analysis is provided by DeCyder 2 D Differential Analysis Software with or without DeCyder Extended Data Analysis Software module or with ImageMaster 2D Platinum v6 0 DIGE Enabled software Internal standard and experiment
88. f 2 D gels by preventing streaking e Eliminates extra spots caused by nonspecific oxidation of proteins e Stabilizes proteins throughout entire 2 D runs ensuring simplified well resolved protein patterns e Ideal for both analytical and preparative gels The appearance of streaks that distort 2 D electrophoresis maps is q common problem occurring most frequently when running gels that contain regions greater than pH 7 0 Increased sample load increased length of the IPG strip or using a narrower pH gradient worsen the problem Extra spots on 2 D gels caused by nonspecific oxidation of proteins is another difficulty encountered when running gels containing basic regions Both streaking and nonspecific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining protein thiol groups in a single oxidation state irrespective of sample load pH range or run length Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis GE Healthcare DIGE approved ORDERING INFORMATION Product Quantity Code Number DeStreak Rehydration Solution 5x3ml 17 60
89. folds can handle 12 IPG strips simultaneously Sample Ettan IPGphor Manifolds are high throughput accessories for equilibration can also be performed in the same tray saving first dimension isoelectric focusing of proteins using IPG strips substantial time These manifolds meet the increasingly stringent demands of proteomics research by ensuring high resolution micropreparative protein analysis Ettan IPGphor Manifold Light is constructed from a new leading edge polymeric material and is ideal for routine general screening applications It is easy to handle and is extremely Ettan IPGphor Manifold and Manifold Light simplify isoelectric robust focusing of multiple samples and reduce first dimension hands on time to a minimum They significantly speed up the processing of micropreparative loads in first dimension analysis The ceramic Ettan IPGphor Manifold exhibits exceptionally uniform thermal characteristics and is designed for the most stringent applications The high heat conductivity of the ceramic material prevents any hot spots that can distort 2 D electrophoresis maps GE Healthcare For more information visit www gelifesciences com 215 n QO O Z O O i ad Q LL 274 2 D Electrophoresis Second Dimension Ettan DALTsix Large Vertical System The compact six gel capacity Ettan DALTsix Electrophoresis Unit e Modular system for large format and medium throughput 2 D electrophoresis e Run
90. g information visit www gelifesciences com orderonline e 200 V 2000 mA 200 W maximum e Constant voltage constant current or constant power modes e Single unit increments in settings and read outs for precision and reproducibility e Print log of run parameters via RS232 port e Designed for high current applications such as tank blotting e Stores and recalls three protocols Related Products Protein Blotting Refer To Chapter 9 ORDERING INFORMATION Product Quantity Code Number Adapter set 2 mm 4 mm for use with 2 mm HV leads to 1 18 1129 58 4 mm power supply output jacks Adapter set 4 mm 2 mm for use with 4 mm HV leads to 1 18 1129 59 2 mm power supply output jacks For pricing information visit www gelifesciences com orderonline e Choose adapters for connecting 4 mm or 2 mm leads to 2 mm or 4 mm output jacks respectively e Use Adapter Set 4 mm 2 mm for connecting sheathed 4 mm male plug instrument leads to 2 mm high voltage output jacks e Use Adapter Set 2 mm 4 mm for connecting sheathed 2 mm male plug instrument leads to 4 mm sheathed output jacks To use GE Healthcare electrophoresis units rated less than 1000 V with GE Healthcare power supplies rated at more than 1000 V use Adapter Set 4 mm 2 mm To use GE Healthcare electrophoresis units rated 1000 V and higher with GE Healthcare power supplies rated at less than 1000 V use Adapter Set 2 mm 4 mm For more information v
91. gel and on the blot e The specific activity of the 14C version without Rainbow labeling is 0 37 3 7 MBq mg 10 100 uCi mg or 185 kBq ml 5 uCi ml supplied in aqueous buffer e The specific activity of the Rainbow labeled version is 0 37 3 7 MBq mg 10 100 uCi mg or 148 kBo ml 4 uCi ml supplied in 50 glycerol SELECTION GUIDE 14C Protein Molecular Weight Markers Protein M CFA626 CFA645 CFA755 CFA756 Myosin 220000 g Phosphorylase b 97000 e BSA 66000 Ovalbumin 45000 e e e Carbonic anhydrase 30000 e e Trypsin inhibitor 20100 e e Lusozume 14300 e e e Cutochrome c 12500 Aprotinin 6500 Insulin B choin 3500 g Insulin A chain 2500 e Under non denaturing conditions the 2500 and 3500 will not be observed pl Markers Components of pl Calibration Kits Kit Protein pl Native Broad pl Kit Amulglucosidase 3 50 pl 3 5 9 3 Methyl red dye 3 75 Trypsin inhibitor 4 55 B Lactoglobulin A 5 20 Carbonic anhudrase B bovine 5 85 Carbonic anhydrase B human 6 55 Myoglobin acidic band 6 85 Myoglobin basic band 7 35 Lentil lectin acidic 8 15 Lentil lectin middle 8 45 Lentil lectin basic 8 65 Trypsinogen 9 30 Low pl Kit Pepsinogen 2 80 pl 2 8 6 5 Amulglucosidase 3 50 Methul red dye 375 Glucose oxidase 4 25 Trypsin inhibitor 4 55 B Lactoglobulin A 5 20 Carbonic anhydrase B bovine 5 85 Carbonic anhydrase B human 6 55 High pl Kit B Lactoglobulin A 5
92. hcare Electrophoresis Standards and Reagents Pulsed Field Gel Electrophoresis Immobiline for IEF and Carrier Ampholytes PlusOne Ammonium Persulfate Ammonium persulfate is the initiator of choice for the polymerization of acrylamide The high quality of PlusOne Ammonium Persulfate supports efficient polymerization at concentrations as low as 1 mM TECHNICAL SPECIFICATIONS M 228 20 Minimum assay gt 98 PlusOne Bind Silane Bind Silane y methacryloxypropultrimethoxysilane is used to covalently attach polyacrylamide gels to a glass surface The gel stays firmly attached to the glass during staining and drying procedures PlusOne Boric Acid TF Sms plusone Moric Acid Shuga gamp w 2 PlusOne Boric Acid Boric acid is for the preparation of TBE buffer the most frequently used buffer for DNA RNA electrophoresis Bromophenol Blue Bromphenol Blue is an electrophorisis tracking dye CHAPS A nonionic or zwitterionic detergent is included in the sample solution to ensure complete sample solubilization and to prevent aggregation through hydrophobic interactions CHAPS is a zwitterionic detergent commonly used in IEF GE Healthcare DIGE approved ORDERING INFORMATION Product Quantity Code Number PlusOne Ammonium Persulfate 25g 17 1311 01 For pricing information visit www gelifesciences com orderonline Store dark and dry at room temperature OGE approved Quantity Code Numb
93. he co polymerization of glycine glycylglycine amines and epichlorohydrin are available in five broad range and four narrow range pH intervals Each interval contains numerous ampholytes with a high buffering capacity per pH unit Pharmalyte carrier ampholytes form an extremely stable linear pH gradient exhibiting even conductivity across the gel For precast PhastGel gels for PhastSystem see page 299 For more information visit www gelifesciences com The Immobiline System is recommended when extremely high resolution and time stable linear pH gradients are required e g 2 D electrophoresis Pharmalyte Carrier Ampholytes are excellent for both analytical and preparative isoelectric focusing ORDERING INFORMATION Product Quantity Code Number Immobiline Il pK 3 6 10ml_ 80 1255 70 Immobiline Il pK 4 6 10ml_ 80 1255 71 Immobiline II pK 6 2 10ml_ 80 1255 72 Immobiline Il pK 7 0 10 ml 80 1255 73 Immobiline II pK 8 5 10m 80 1255 74 Immobiline Il pK 9 3 10ml 80 1255 75 For pricing information visit www gelifesciences com orderonline Each bottle contains a ready to use 0 2 M solution Store at 4 8 C For Immobiline DryStrip Gels and IPG Buffers see page 269 ORDERING INFORMATION Product Quantity Code Number Pharmalyte broad range pH 2 5 5 25ml 17 0451 01 Pharmalyte broad range pH 4 6 5 25ml 17 0452 01 Pharmalyte broad range pH 5 8 25ml 17 0453 01 Pharmalyte broad range pH 8 10 5 25ml 17 0455 01 Pharmalyt
94. he SE 600 Ruby can handle up to four gels 14 or 16 x 16 cm up to 1 5 mm thick simultaneously The average separation time is 4 5 h miniVE Vertical Electrophoresis System offers speed and economy for second dimension separations With a 90 min run time and the ability to run two 8 x 9 5 cm gels simultaneously miniVE enables rapid screening using 7 cm IPG strips see page 283 Related products for proteome studies A wide variety of related products are available for sample preparation subsequent handling and analysis of 2 D gels Sample preparation is simplified by the Ettan and PlusOne ranges of kits and reagents See Chapter 5 designed to provide consistent 2 D results quickly and reliably For protein labeling and detection products blotting membranes transfer units and automated gel staining and blot processing using Processor Plus see Chapter 9 For gel and blot imaging and analysis see Chapter 10 Tap into 2 D knowledge In addition to sponsoring seminars workshops and conferences around the world on 2 D electrophoresis a wide variety of 2 D support material is available from GE Healthcare code numbers listed in parentheses Handbooks 2 D Electrophoresis Using Immobilized pH Gradients Principles and Methods 80 6429 60 is q comprehensive lab handbook containing information on sample preparation detailed running conditions for first and second dimension systems troubleshooting and references Application
95. iable detection and high sensitivity For more information visit www gelifesciences com GE Healthcare 2 D Electrophoresis Ettan Spot Picker Ettan Spot Picker performs accurate and reliable picking of gel electrophoresis spots e Automatically picks selected protein spots from stained or destained gels with greater than 99 9 efficiency e Designed for use with backed and nonbacked gels e Compatible with common detection systems and scanners e Seamless integration with DeCuder 2 D Differential Analysis Software Ettan Spot Picker is a robotic system designed to accurately pick protein spots from 2 D gels and transfer the picked proteins into microplate wells in preparation for further analyses such as digestion and protein identification by MALDI mass spectrometry Polyacrylamide 2 D gels stained with common detection stains such as silver Coomassie or fluorescent dyes are scanned and data can be analyzed using ImageMaster 2D Platinum Software or DeCyder 2 D Differential Analysis Software A protein picklist is generated either automatically using predefined selection criteria or manually The protein picklist is transferred to Ettan Spot Picker which then automatically picks the protein spots according to the list Attaching self adhesive reference markers to the gel backing prior to scanning the gel ensures a precise link between electronic image and actual gel A wash step is performed after each picking
96. iline DryStrip pH 6 11 11 cm 12 17 6001 95 Immobiline DryStrip pH 3 10 11 cm 12 18 1016 61 Immobiline DryStrip gels were rehydrated in DeStreak Rehydration Solution with Immobiline DryStrip gels 7 cm PG Buffer solution cs indicated Second dimension Eton DALToweNvevsing ALT GA 12 arson or ovals of the same color ee on rs broad pH gradient 311 NL and the Immobiline 5 Tip Bee ce cepa elas medium range 7 11 NL indicate the same protein groups Yellow ring alkylated Immobiline DryStrip pH 6 2 7 5 7 cm 1G IEEE lysozyme alkylated lysozyme pl 10 5 calculated according to SwissProt Immobiline DryStrip pH 7 11 NL 7 cm 12 17 6003 68 A Broad range Immobiline DryStrip pH 3 11 NL 24cm using 0 5 Buffer 3 11 NL Immobiline DryStrip pH 3 11 NL 7 cm 12 17 6003 73 run for 42 kVh Sample 100 ug mouse liver extract 7 5 ug alkylated lysozyme Immobiline DryStrip pH 4 7 7 cm 12 17 6001 10 cup application at the anode Immobiline DryStrip pH 6 11 7 cm 12 17 6001 94 B Medium range Immobiline DryStrip pH 7 11 NL 24 cm using 0 5 IPG Buffer Immobiline DryStrip pH 3 10 NL 7 cm 12 17 6001 12 7 11 NL run for kVh Sample 100 ug of mouse liver extract 5 ug alkylated me beet es 12 17 6001 11 lysozyme cup application at the anode For pricing information visit www gelifesciences com orderonline Increased resolution between pH 5 and pH 7 GE Healthcare For more information visit www gelifesciences com 269 2 D Electrophoresis First
97. ing 75 105 kPa up to 2000 m altitude 100 120 V or 220 240 V 10 50 60Hz single phase Spotting capacity Process capacity Operating temperature Maximum relative humidity Air pressure Mains voltage Mains fuse 2A 110V or 1A 220 V Power consumption Mox 200 VA Safety UL 1260 CSA C22 2 No 151 IEC 1010 1 EMC EN 50082 1 FCC Class A EMI EN 50081 1 Dimensions W x D x H 91 x 61 x 56 cm Weight 41 kg GE Healthcare SDS PAGE IEF Introduction to Vertical Electrophoresis Units SELECTION GUIDE BY TECHNICAL OVERVIEW Vertical Electrophoresis Units Gel Size Glass Plate Size Heat Maximum Model W x L cm W x L cm No of Gels Exchanger Samples Gels Mini format miniVE 8 x 9 5 10 x 10 5 1 2 N 18 SE 250 8x7 10x8 1 2 Y 18 SE 260 8 x 7 or 9 5 10 x 8 or 10 5 1 2 Y 18 Standard 16 cm long format SE 400 14 x 16 18 x 16 1 2 N 28 SE 600 Ruby 14x 16 or 8 18x 160r8 1 4 Y 28 Standard 24 cm long format SE 660 14x 24 16 0r8 18 x 24 16 or 8 1 4 Y 28 Optional divider plate doubles capacity For general information concerning electrophoresis of proteins see Technical Appendix m D TECHNICAL SPECIFICATIONS re Safety certifications All Vertical Units have the following safety Fr certifications CE 73 23 EEC LV directive JR EN 61010 1 IEC1010 1 UL3101 1 O CSA22 2 1010 1 O gt me miniVE Vertical Electrophoresis System O N ORDERING INFORMATION 2 Product
98. ips H IEF sample application pieces ORDERING INFORMATION Product Quantity Code Number SDS and Native PAGE IEF Casting Kit 1 18 1102 45 Includes Glass plate 125 x 260 x 0 5 mm U frame 2 80 1106 89 Glass plate 260 x 125 x 1mm 15 80 1106 29 Glass plate 260 x 125 x 3 mm 2 80 1106 99 FlexiClamps 6 18 1013 73 IEF electrode strips 100 18 1004 40 EPH electrode wicks paper 104 x 253 mm 500 80 1129 52 Sample application pieces 200 80 1129 46 IEF SDS sample application strips 52 samples 5 20 ul 5 18 1002 26 Cellophane sheets 210 x 320 mm 50 80 1129 38 For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product Quantity Code Number Immobiline DryStrip Kit 1 18 1004 30 Includes Electrode anode 1 18 1018 66 Electrode cathode 1 18 1018 67 Sample cup bar 1 18 1004 33 Tray and electrode holder 1 18 1004 31 Sample cups Immobiline DryStrip Kit 60 18 1004 35 DryStrip aligners 4 18 1004 34 IEF electrode strips 100 18 1004 40 Sample application pieces 200 80 1129 46 For pricing information visit www gelifesciences com orderonline Related Products IPGbox NEW IPGbox Kit NEW Immobiline DryStrip Gels Immobiline DryStrip Cover Fluid Code Number 28 93 34 65 28 93 34 92 17 1335 01 Refer To page 271 page 271 page 269 page 311 For more information visit www gelifesciences com GE Healthcare NovaBlot Kit General Accessories ORDERING INFORMATION
99. isit www gelifesciences com udo112 3 O x O T 305 n Q O O O i jad Q LL 306 Electrophoresis Standards and Reagents Electrophoresis Reagents e Optimized quality for each electrophoresis technique SELECTION GUIDE Standards and Reagents continued e Strict specification control Product Use Page e Batch testing to ensure reproducibility PlusOne Glass Plate Treatment High Quality Electrophoresis Chemicals a complete range of aa a ies Segoe chemicals for electrophoresis a a Other PlusOne Reagents SELECTION GUIDE Standard dR i Immobiline DryStrip Cover Fluid 2 D Immobiline DryStrip 311 Bee OE ie eee Amberlite IRN 150L Deionizing Solutions 308 Product Use Page Agaroses DNA RNA IEF 308 rise Gel Casting Chemicals Carrier Ampholutes and Immobiline for IEF Acrylamide IEF EF PAGE Sequencing eve Pharmalyte Carrier Ampholytes IEF 314 Acrylamide IEF 40 Solution IEF PAGE 308 Immobiline II System lee 314 0 0 A ni 40 T 3 C IEF ane Protein Molecular Weight and pl Markers mney a Brae f ass ve Protein MW Markers PAGE 315 Acrylamide PAGE 40 Solution PAGE 308 Rambow MN MOr PAGE 316 ReadySol DNA PAGE 40 T 5 C PAGE Sequencing 308 ECL Western Blotting MW Markers PAGE 318 N N Methylenebisacrylamide IEF PAGE Sequencing 311 Protein Moleculor Weight PAGE 318 N N Methylenebisacrylamide 2 IEF PAGE Sequencing 311 Standards POO
100. ity 80 noncondensing Air pressure 75 105 kPa up to 2000 m altitude 110 120 VAC 10 50 60 Hz or 220 240 VAC 10 50 Hz single phase installation category ll Mains voltage Mains fuse 2 A 110 VAC or 1 A 220 VAC Power comsumption Max 200 VA Safety UL 1260 UL 1492 camera CSA C22 2 No 151 IEC 1010 1 EMC EN 50082 1 EN 50022 framegrabber FCC Class A EMI EN 50081 1 EN 50024 framegrabber Dimensions W x D x H 91 x 61 x 56 cm Weight 41 kg For more information visit www gelifesciences com udo1125 3 O x OD T 279 n Q O S O Q LL 280 2 D Electrophoresis Automated Gel Handling Ettan Spot Picker Nonbacked Gel Kit w Automated spot picking using the Etton Spot Picker Nonbacked Gel Kit e Allows quick and accurate protein spot picking from nonbacked 2 D gels increasing the versatility of Ettan Spot Picker e Compatible with common staining methods such as silver Coomassie Blue fluorescence and labeling with Ettan DIGE Fluor dyes e High precision picking with reference markers using the Gel Frame a nonbacked gel slab is attached to a temporary cellophane backing supporting the reference markers e Improves nonbacked gel handling during scanning and the Glass Lid protects the moist gel For more information visit www gelifesciences com ORDERING INFORMATION Product Ettan Spot Picker Nonbacked Gel Kit Porous Cellophane
101. l Homogeneous High density 10 17 0679 01 PhastGel IEF 3 9 10 17 0543 01 PhastGel IEF 4 6 5 10 17 0544 01 PhastGel IEF 5 8 10 17 0545 01 PhastGel Dry IEF 10 17 0677 01 PhastGel cassette 1 18 1001 01 Application Kits CSF Analysis Kit 1 18 1039 14 PhastGel Staining Kits PlusOne Coomassie Tablets PhastGel Blue R 350 40 tablets 17 0518 01 PlusOne Silver Staining Kit Protein 1 17 1150 01 PhastGel DNA Silver Staining Kit 1 17 1596 01 PhastGel Sample Applicators PhastGel Sample Applicators 12 x 0 3 ul 50 18 1614 01 PhastGel Sample Applicators 8 x 0 5 pl 50 18 1617 01 PhastGel Sample Applicators 8 x 1 ul 50 18 1618 01 PhastGel Sample Applicators 6 x 4 ul 50 18 0012 29 Sample well stamp 1 18 0097 01 For pricing information visit www gelifesciences com orderonline PhastGel media buffer strips and Protein Silver Staining Kit should be stored at 4 C to 8 C DNA Silver Staining Kit should be stored at room temperature Lane 1 Low Molecular Weight Calibration Kit LMW Lane 2 Starting material cytoplasmic extract dil 1 20 Lane 3 Flow through dil 1 10 Lane 4 Wash Lane 5 Eluted GST His M 94 000 dil 1 20 67 000 Lane 6 Eluted GS I His 43 000 dil 1 10 Lane 7 GST standard 30 000 0 5 mg ml Lane 8 LMW 20 100 14 400 Lane 1 2 3 45 6 T 8 SDS PAGE of histidine tagged glutathione S transferase purified from a cytoplasmic extract using HisTrap Kit and FPLC Analysis of sample f
102. lamide IEF is outstanding for use in isoelectric focusing and DNA sequencing In addition to the absence of polymerization interfering impurities Acrylamide IEF is essentially free of charged acrylamide derivatives lt 20 ppm for very stable pH gradients PlusOne Acrylamide is available as a powder a solution and premixed solutions ReadySol Powder Dry powder is the most stable form of acrylamide with a shelf life of more than three years TECHNICAL SPECIFICATIONS Acrylamide IEF PAGE M 71 08 71 08 Minimum assay gt 99 9 Acrylic acid lt 0 002 lt 0 05 Agaroses DNA RNA Techniques Agarose with low electroendosmosis EEO is recommended for most DNA RNA applications If separated nucleic acids are to be used as substrates for enzymes e g ligase and restriction endonucleases a special highly purified agarose such as Agarose NA should be used For preparative work an agarose with low gelling temperature should be used Agarose Prep has both low melting and gelling points IEF Techniques Agarose IEF is suitable for isoelectric focusing of large molecules and is the gel of choice for many IEF applications and immunochemical detection methods ORDERING INFORMATION Product Quantity Code Number PlusOne Acrylamide PAGE 250g 17 1302 01 PlusOne Acrylamide PAGE 1kg 17 1302 02 Acrylamide PAGE 40 Solution 11 17 1303 01 PlusOne ReadySol DNA PAGE 40 T 5 C 11 17 1308 01 PlusOne Acrylamide IEF
103. latbed Units SELECTION GUIDE BY TECHNICAL OVERVIEW Multiphor II Application Kits Components and Accessories Technique Size Accessory Kit Page Cast gradient gels 100 ml total volume Gradient maker 293 Cast IEF acrylamide gels 258 x 124 x 0 5 mm SDS and Native PAGE IEF Casting Kit this page Position and hold ExcelGel Buffer Strips 24x 11 cm and 24x 18cm Multiphor Il Buffer Strip Positioner 295 Rehydrate run Immobiline DryStrip for IPG 2 D 70 110 130 180 or 240 mm strips Immobiline DryStrip Kit and Reswelling Tray this page Semi dry blotting 20 x 25 cm NovaBlot Kit and Film Remover 293 For general information concerning electrophoresis of proteins see Technical Appendix SDS amp Native PAGE IEF Kit F H G SDS and Native PAGE IEF Casting Kit components A Glass plates 0 5 mm thick U frame E IEF electrode strips B Glass plates 1 mm thick F EPH electrode wicks C Glass plates 3 mm thick G IEF sample application pieces D FlexiClamps H IEF SDS sample application strips I Cellophane sheets Immobiline DryStrip Kit H F T ke ie ly E a D G ia k z e For use of Immobiline DruStrip gels with Multiphor II Electrophoresis Sustem Immobiline DruStrip Kit components A Electrode anode B Electrode cathode C Sample cup bar D Tray and electrode holder Please note that this kit can only be used with Multiphor Il E Sample cups F DryStrip aligners G IEF electrode str
104. m orderonline Related Products Refer To EPS 601 Power Supply page 304 MultiTemp III Thermostatic Circulator page 276 2 D Fluorescence Difference Gel Electrophoresis page 262 Immobiline DryStrip Gels page 269 Ettan IPGphor 3 IEF System page 272 Ettan DALTtwelve Large Vertical System page 277 Ettan DALTsix uses the same convenient pre assembled gel cassette designs with hinged plates and permanent spacers as the original DALT and Ettan DALTtwelve systems For lab cast gels choose either a 1 0 mm or 1 5 mm gel casting cassette and high quality PlusOne gel casting chemicals For added convenience choose precast DALT Gel 12 5 and the 1 0 mm precast gel cassette Precast DALT gels are barcoded for convenient sample and batch processing Brussel sprout Brassica oleracea vor Gemnifera extract separated in the first dimension on an Immobiline DryStrip pH 3 10 NL 24 cm The second dimension was run on Ettan DALTsix with a 12 5 T 2 7 C polyacrylamide gel using the Laemmli buffer gt ee See sustem GE Healthcare 2 D Electrophoresis Second Dimension Ettan DALTsix Large Vertical System continued DALTsix Gel Caster and Gradient Maker TECHNICAL SPECIFICATIONS DALTsix Gel Caster holds up to six 1 0 mm or 1 5 mm cassettes with separator sheets for casting homogeneous or gradient gels If fewer gels are to be cast the unused volume is filled with blank cassette inserts and filler sheets m D O et x aaa r
105. ment Parts Comb back for HE 99X combs with 2 screws 1 80 6054 35 z D Foam sealing gaskets 4 80 6061 19 HE 99X Gel Casting Kits N Kit 15 x 10 cm 1 80 6060 62 ada z Kit 15 x 15 cm 1 80 6060 81 Kit 15 x 20 cm 1 80 6061 00 HE 99X Max Submarine Unit fir multiple gel lengths and durable service Wells D e Versatile submarine unit is ideal for any molecular biology Thickness Width 0 lab No mm mm Quantity Code Number O e Run as many as 60 samples at once ere 10 1 5 oe 1 80 6055 68 C Costing kits require no tope or dams E 7 uo O Multichannel pipettor compatible combs available ia a r 55 eee HE 99X Max Submarine Unit offers the best value for general 1 Ta i u use ee par applications use in a standard sized format n oe it 9 61 20 1 0 4 7 1 80 6056 63 UO 20 LS 4 7 1 80 6056 82 K 20 3 0 4 7 1 80 6057 01 LLJ 30 1 0 3 0 1 80 6057 20 1 21 1 5 113 10 1 80 6057 58 1 21 60 113 10 1 80 6057 77 Multichannel Pipette Compatible Combs for HE 99X 14 1 0 6 0 1 80 6460 38 14 1 5 6 0 1 80 6460 57 N For pricing information visit www gelifesciences com orderonline Without back Order 80 6054 35 comb back separately f Preparative reference wells w Related Products Code Number Refer To EPS 301 Power Supplu 18 1130 01 page 304 Agaroses page 308 DNA Molecular Weight Markers page 320 ImageQuant Imagers page 384 Gel casting kits for HE 99X require no tape or dams TECHN
106. n disease stages tumor types or other sample subtypes e Integrates data from multiple Biological Variation Analysis BVA workspaces Helps you understand regulatory pathwaus Integrates data from external databases DeCyder Extended Data Analysis Software DeCyder EDA is high performance informatics software for the analysis of large and combined proteomics data sets DeCyder EDA was developed specifically for 2 D DIGE methodology and extends the statistical options offered in DeCyder 2 D Differential Analysis Software DeCyder 2 D This software enables the seamless integration of DeCyder EDA statistical results with DeCuder 2 D visualization data thereby placing the results into biological context by linking to internal and external databases For more information visit www gelifesciences com OGE approved ORDERING INFORMATION Product Quantity Code Number DeCyder Extended Data Analysis Software one network 1 28 4012 03 user license DeCyder Extended Data Analysis Software one additional 1 28 4027 11 network user license DeCyder Extended Data Analysis Software four 1 28 4012 05 additional network user licenses DeCyder Extended Data Analysis Software 1 month trial 1 28 4017 86 license DeCyder Extended Data Analysis Software 6 month trial 1 28 4020 87 license For pricing information visit www gelifesciences com orderonline Related Products Code Number Refer To DeCyder 2 D Differential Analysi
107. ndling and viewing e Combs allow up to 32 samples per run HE 33 Mini Submarine Unit has an enhanced passive cooling system allowing you to run gels at high voltages for unparalleled speed and resolution in a mini gel submarine unit The novel molded base is designed to be filled with q common coolant such as 50 ethylene glycol so that it readily maintains short term cooling capacity after chilling in a freezer This allows for highly efficient heat exchange 1 2 3 4 5 6 ri 8 23 130 bp Separation of DNA size markers in HE 33 Submarine Unit ar Lanes 1 4 7 X 174 RF DNA Hindll digest lanes 2 5 8 lambda 1 078 bp DNA Hindlll digest lanes 3 6 dX 174 RF DNA Haelll digest Markers were separated for 30 min at 200 Vina1 agarose gel GE Healthcare Standard Format Submarine Units ORDERING INFORMATION Product Quantity Code Number HE 33 Mini Submarine Unit Basic 1 80 6052 64 Includes Gel running tray gel casting tray and bubble level Order comb and comb back separately HE 33 Mini Submarine Unit Complete 1 80 6052 45 Includes Basic unit 1 5 mm thick 8 well comb and comb back Accessories and Replacement Parts Buffer chamber assembly 1 80 6050 17 Comb back with 2 screws for HE 33 combs 1 80 6050 36 Lid with power cables 1 80 6052 83 Filling plug top 4 80 6412 31 Filling plug bottom 1 80 6053 02 Gel running tray UVT 7 x 10 cm 1 80 6053 40 Casting tray 7 x 10 cm 1 80 6053 59 G
108. ng spacers pedi low fluorescence glass for use with fluoroscent dyes OD DALT Glass Plate Set including spacers 1 80 6475 39 Q regular glass plates for spot picking or DALT Blank Cassette Insert 1 80 6467 03 JR DALTtwelve Gel Caster complete 1 80 6467 22 O DALT Separator Sheets 0 5 mm 16 80 6467 41 O DALT Filler Sheets 1 0 mm 6 80 6467 60 gt Ettan DALTtwelve System can run simultaneously up to 12 large format gels in DALT Cassette Removal Tool 2 80 6474 82 O ato6h DALT Buffer Seal Removal Tool 2 80 6474 63 e Highest capacity electrophoresis unit with the largest DALT Cassette Rack TO I precast gels available 26 x 20 cm DI emmy gt x aa sm Stable discontinuous buffer system ina precast DALT Gradient maker with Peristaltic Pump 115 VAC 1 80 6067 65 Tp DALT Gradient maker with Peristaltic Pump 230 VAC 1 80 6067 84 homogeneous gel features high batch to batch reproducibility For pricing information visit www gelifesciences com orderonline e Simple to use gel cassettes for gel loading unloading and Related Products Refer To sto rage 2 D Fluorescence Difference Gel Electrophoresis this chapter e Programmable power supply and temperature control Ettan DALTsix Large Vertical System pee ensure high reproducibility among runs a a a o page ww s hoe od mmobiline DruStrip Gels page e Sustem design minimizes buffer consumption sns uo mh a Ettan DALTtwelve System streamlines performing second dimension SDS PAGE sep
109. nk Protects gel plates from chipping and cracking during handling and storage Adjusts for plates from 8 x 10 cm to 18 x 16 cm Holds ten 10 18 cm plates or twenty 8 cm plates SE 100 Plate Washer is an ideal accessory for vertical units with glass plates up to 18 x 16 cm 290 For more information visit www gelifesciences com ORDERING INFORMATION Product Quantity Code Number SE 100 Plate Washer and Storage Unit 1 80 6116 29 Includes 2 molded plate holders with handles 2 long plate adapters 2 dust covers and 1 polypropylene washing tank with lid Long plate adapter for 10 cm and 12 cm long plates Molded plate holder with handle and dust cover 2 80 6116 48 1 80 6116 67 For pricing information visit www gelifesciences com orderonline GE Healthcare SDS PAGE IEF Multiphor II Electrophoresis System Multiphor II Flatbed Electrophoresis System is designed for 1 D or 2 D electrophoresis The system shown here is comprised of from left to right EPS 3501 XL Power Supply Multiphor II Electrophoresis Unit center front MultiTemp III Thermostatic Circulator center rear and Processor Plus e Configure the system for 1 D or 2 D protein electrophoresis or DNA fragment analysis e Run up to 12 IPG strips 7 24 cm simultaneously e Select from a wide range of precast gels or cast your own gels for unique applications e Allows cooling of ultra thin gels efficiently and uniformly through the
110. o pairs of power outlets for duplicate parallel runs General guidelines for using power supplies EN 61010 1 e The constant current mode is recommended for blotting to m prevent overheating OD e The constont voltqge mode is recommended for other e applications such as nucleic acid submarine and mini ma vertical gel electrophoresis O e When connecting two electrophoresis units to the same O power supply twice as much current is required at the same e voltage O e To maintain a constant temperature without using a thermostatic circulator use the constant power mode D EPS Power supplies are the perfect tools for electrophoresis and blotting N applications Ta SELECTION GUIDE BY TECHNICAL OVERVIEW Power Supplies by Performance EPS 301 EPS 601 EPS 1001 EPS 3501 XL EPS 2A200 Output range Voltage V 5 300 6 600 5 1000 35 3500 0 200 5 V increments Current mA 10 400 1 400 1 400 1 400 0 2000 Power W 80 max 1 100 1 100 1 200 1 200 Timer run end 1 min 24 h 1 min 500 h 1 min 500 h 1 min 500 h 0 999 min continuous continuous continuous continuous continuous 1 Vh 500 kVh 1 Vh 500 kVh 1 Vh 500 kVh 1 mAhr 25 Ahr Output connector type 4mm 4mm 2mm 2mm 4mm Programs stored 3 protocols 1 phase 3 protocols 1 phase 9 protocols 9 phases each 3 protocols 1 phase AC power 100 120 V 100 120 V 100 120 V 100 120 V 90 132 requirement mains 220 240 V 220 240 V 220 240 V 220 240 V 180 26
111. ory divider plate s 1 When using 13 cm IPG strips you will need 1 cm spacers t For 2 D DIGE work use low fluorescence glass plates GE Healthcare For more information visit www gelifesciences com 261 n Q O S O Q LL 262 2 D Electrophoresis 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE Introduction to 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE using Ettan DIGE System Internal standard label with Cy2 v v gt gt Mix labeled samples lt q 4 Protein sample 1 eg control label with Cy3 Protein sample 2 e g treated label with Cy5 Separate by 2 D PAGE a ew Sa u a p ml Gea 7 P p Excitation wavelength 1 Excitation wavelength 2 Excitation wavelength 3 v a Analysis of differences with lt lt DeCyder gt gt ss 5 Differential Analysis Software Image analysis data quantitation Image analysis overlay images Workflow for Ettan DIGE system showing co separation of three fluorescently labeled samples separated on a single 2 D gel One sample is an internal standard e Detect an increased number of real differences in protein expression e Identify the smallest possible expression differences e Guarantee statistical confidence e Eliminate gel to gel variation Ettan DIGE 2 D Fluorescence Difference Gel Electrophoresis system enables the prelabeling and separation of up to three sampl
112. pH 3 7 NL and pH 6 9 or select from 3 5 6 NL 5 3 6 5 6 2 7 5 and 7 11 NL when more detail is required e Use narrow pH range strips 1 pH unit to closely study proteins in the regions of interest 2 70 For more information visit www gelifesciences com GE Healthcare IPG Buffers e For use with Immobiline DruStrip gels to improve protein solubilitu e Ensures optimal conditions for IEF separation e Eliminates background staining IPG Buffers are ampholyte containing buffer concentrates specifically formulated for use with Immobiline DryStrip gels Each IPG Buffer type produces more uniform conductivity along the Immobiline DryStrip during focusing resulting in wider latitude in run times IPG Buffers also eliminate high background staining IPG Buffers are supplied in 1 ml aliquots of water The buffer is diluted 50 or 200 fold in the rehydration solution depending on the first dimension system and pH range of the strip SELECTION GUIDE pH Ranges of Immobiline DryStrip Gels qnd Recommended IPG Buffers pH range IPG Buffer pH range Immobiline DruStrip Gel Sl 4 l 3 5 5 0 4 5 5 5 5 0 6 0 5 5 6 7 7 11 NL 3 10 NL 3 10 3 11 NL ji 3 5 6 NL 5 3 0 5 6 2 7 5 e 7 11 NL e 3 11 NL e 3 5 4 5 e 4 0 5 0 e 4 5 5 5 5 0 6 0 e 5 5 6 7 e 3 7 NL e 4 7 e 6 9 e 6 11 3 10 e 3 10 NL IPGbox and IPGbox Kit NEW ay v IPGbox is designed for simultaneous rehyd
113. page 290 TE 22 Mini Tank Transfer Unit 80 6204 26 page 347 Electrophoresis Reagents page 306 Amersham ECL Plex Western Blotting Combination page 354 Packs Coolant Out Coolant In Cooling Core cut away A serpentine channel passes between the two upper buffer chambers of SE 260 Mini Vertical Unit ensuring efficient cooling Blotting For efficient electrotransfer of up to four gels at once with the TE 22 Mini Tank Transfer Unit see page 347 For more information visit www gelifesciences com udo1125 3 O me OD T 285 n Q O O ad Q LL 286 SDS PAGE IEF Mini Format Vertical Units SE 250 and SE 260 Accessories al ai J Se SE 250 and SE 260 components A Deep lower buffer chamber for 10 5 cm gels B Upper buffer chamber C Lid assembly E Glass plates F Gel seal G Spacers various H Combs various D Mini Vertical Gel Caster I Clamps ORDERING INFORMATION Product Quantity Code Number Clamps Red clamps for SE 260 and gel casters 4 80 6147 83 Electrophoresis Unit Replacement Parts Foam gasket 4 5 mm o d x 61 cm long 1 80 6137 19 Upper buffer chamber for SE 250 and SE 260 1 80 6148 40 Lower buffer chamber for SE 250 1 80 6148 59 Deep lower buffer chamber for SE 260 1 80 6148 78 Lid with cables for SE 250 and SE 260 1 80 6149 16 Wonder Wedge plate separation tool 1 80 6127 88 Gel Seal 1 80 6421 43
114. parameters are suggested by merely entering data relating to the number and type of Immobiline DryStrips being used GE Healthcare 2 D Electrophoresis First Dimension Ettan IPGphor 3 IEF System continued Ettan IPGphor 3 Control Software TECHNICAL SPECIFICATIONS Ettan IPGphor 3 Control Software generates professional graphical reports with a large user interface for programming and editing The user can create save and edit protocols and also pause during a run to edit a protocol in real time The software can be used to control up to four Ettan IPGphor 3 IEF units simultaneously each running a different set of run parameters The units can be started stopped or paused using the PC control unit The software enables web browser remote monitoring of the PC control unit Stored log files of previous runs can be opened and viewed and status reports can be generated along with instantaneous run condition reports on request Log files can be exported for example to Microsoft Excel The software monitors voltage current and volt hours of m the run and generates a graphical display as the run proceeds OD Reports can be created saved printed and exported O O Oo gt O 3 OD N Ettan IPGphor Manifold is a cup loading accessory for Ettan IPGphor IEF unit allowing electrophoresis of large sample loads on IPG strip lengths from 7 24 cm Designed for maximum performance and flexibility the Ettan IPGphor Manifolds mani
115. r and secondly a standard magnetic stirrer circulates buffer around the vertically suspended heat exchanger to maintain homogeneous lower buffer temperature The ergonomically designed SE 600 Ruby provides two built in side handles which facilitates carrying and handling GE Healthcare Standard Format Vertical Units OGE approved ORDERING INFORMATION Product Quantity Code Number Dual Cooled Vertical Gel Units for 18 x 16 cm plates SE 600 Ruby Standard Dual Cooled Vertical Unit Complete 1 80 6479 57 Includes 3 sets of glass plates four 16 cm clamp assemblies 6 cams dual gel casting stand with levelling base and level buffer dam Spacer Mate alignment template Wonder Wedge plate separation tool two 15 well combs and two sets of 1 5 mm thick spacers for 18 x 24 cm plates SE 660 Dual Cooled Vertical Unit Basic 1 80 6189 82 Includes 3 sets of glass plates four 16 cm and four 8 cm clamp assemblies 6 cams dual gel casting stand with leveling base and level buffer dam Spacer Mate alignment template and WonderWedge plate separation tool Order 2 combs and 2 sets of 24 cm spacers separately Glass Plates m 18 x 8 cm g Glass plates 18 x 8 cm 2 80 6186 59 O Glass plates 18 x 8 cm low fluorescence 2 80 6475 77 FF Glass plate club sandwich divider notched 1 80 6186 78 18x 16cm O Glass plates 18 x 16 cm 2 80 6178 99 O Low fluorescence Glass Plates for SE600 18 x 16 cm 2 80 6442 14 3
116. r more information visit www gelifesciences com For pricing information visit www gelifesciences com orderonline Related Products Refer To Nucleic Acid Electrophoresis page 300 e For precise sizing of average sized PCR products and other DNAs from 50 to 500 bp on agarose gels e Supplied at 1 ug ul in TE buffer pH 7 5 in sufficient quantity for 100 loadings using 2 ul per loading after mixing with tracking due e Consists of multiple repeats of a 50 bp fragment resolves to at least 10 distinguishable bands each separated by 50 bp increments when run on a 2 agarose gel e Can be visualized by ethidium bromide staining or by autoradiography after radiolabeling e Bands stain uniformly with ethidium bromide except for the 250 bp band which is approximately twice as intense as the other bands e Not designed for quantitating DNA content in q sample GE Healthcare IEF SDS PAGE amp 2 D Electrophoresis DNA Molecular Weight Markers 100 Base Pair Ladder ORDERING INFORMATION Product Quantity Code Number 100 Base Pair Ladder 100 ul 27 4007 01 For pricing information visit www gelifesciences com orderonline Related Products Refer To Nucleic Acid Electrophoresis page 300 e For precise sizing of double stranded DNA fragments from 100 to 2000 bp on agarose gels Supplied in sufficient quantity for 50 loadings using 2 ul per loading after mixing with tracking dye e Consists of multiple repeats of
117. r more information visit www gelifesciences com ORDERING INFORMATION Product PlusOne Repel Silane ES Quantity Code Number 500ml 17 1332 01 For pricing information visit www gelifesciences com orderonline Store dark and dry at room temperature ORDERING INFORMATION Product Sodium Dodecyl Sulfate m AS z O lt D Q Quantity Code Number 100g 17 1313 01 For pricing information visit www gelifesciences com orderonline m w AS z O lt D Q ORDERING INFORMATION Product PlusOne TEMED Quantity Code Number 25ml 17 1312 01 For pricing information visit www gelifesciences com orderonline Store well sealed dry and dark at room temperature m a EF oO z O lt D Q ORDERING INFORMATION Product Thiourea Quantity Code Number 100g RPN6301 For pricing information visit www gelifesciences com orderonline m D z O lt D Q ORDERING INFORMATION Product Quantity Code Number Tris 500g 17 1321 01 For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product Triton X 100 m D D z O lt D Q Quantity Code Number 500ml 17 1315 01 For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product PlusOne Tween 20 Quantity Code Number 500ml 17 1316 01 For pricing information visit www gelifescience
118. ractions performed on PhastSystem using PhastGel 10 15 and PlusOne Silver Staining Kit Protein For more information visit www gelifesciences com udo1125 3 O x OD T 299 Nn N Y L O Pa O O mur O a Lu 300 SDS PAGE IEF Nucleic Acid Electrophoresis Automated Flatbed Units PhastGel continued PhastGel Gradient Gradient Gradient Homogeneous Homogeneous Homogeneous Homogeneous 4 15 10 15 8 25 7 5 12 5 20 High Densitu Separation range Native M x 103 70 700 80 440 50 300 230 700 80 230 50 100 20 60 SDS M x 103 30 200 15 150 6 150 40 200 20 45 3 25 2 15 Gel description Stacking zone 13 mm 4 5 3 6 3 6 3 5 3 6 3 7 5 2 75 2 Composition T C Separating zone 32 mm 5 15 2 10 15 2 8 25 2 7 5 2 12 5 2 20 2 20 2 Composition T C Buffer A A A A A A B Buffers A 112 mM acetate 112 mM Tris pH 6 4 B 112 mM acetate 112 mM Tris 30 ethylene glycol pH 6 4 Store at 4 C to 8 C TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS PhastGel Buffer Strips PhastGel for IEF Dimensions W x L x H 10 x 41 x 6 mm PhastGel IEF PhastGel Gel moterial 3 agarose IEF 3 9 4 6 5 5 8 Dry IEF Buffer Separation range pl 3 9 4 6 5 5 8 Optionalt SDS 17 0516 01 0 20 u 0 20 M Tris Gel composition T C 5 3 5 3 5 3 i oe PhastGel IEF gels should be stored at 4 8 C Native 17 0517 01 0 88 M L alanine 0 25 M PhastGel Dry IEF should be stored at 20 to 80 C
119. ration of up to 12 Immobiline DruStrip gels e Rehydrate up to 12 Immobiline DryStrip gels or other IPG strips independently and at the same time e Lid protects the IPG strips from dust and contamination e Oil free rehydration e Load samples for IEF during rehydration by including them in the rehydration buffer alternatively load sample using sample cups GE Healthcare 2 D Electrophoresis First Dimension DiGE approved ORDERING INFORMATION Product Quantity Code Number IPG Buffer pH 3 5 5 0 1ml 17 6002 02 IPG Buffer pH 4 5 5 5 1ml 17 6002 04 IPG Buffer pH 5 0 6 0 1ml 17 6002 05 IPG Buffer pH 5 5 6 7 1ml 17 6002 06 IPG Buffer pH 4 7 1ml 17 6000 86 IPG Buffer pH 6 11 1ml 17 6001 78 IPG Buffer pH 7 11 NL 1ml 17 6004 39 IPG Buffer pH 3 10 NL 1ml 17 6000 88 IPG Buffer pH 3 10 1ml 17 6000 87 IPG Buffer pH 3 11 NL 1ml 17 6004 40 For pricing information visit www gelifesciences com orderonline Related Products Refer To Immobiline DryStrip Gels page 269 DeStreak Rehydration Solution page 307 DeStreak Reagent page 307 DIGE approved ORDERING INFORMATION Product Quantity Code Number IPGbox NEW 1 28 9334 65 Includes 1 IPGbox and 1 IPGbox Kit IPGbox Kit NEW 1 28 9334 92 Includes 10 Reswell Trays and 1 IPGbox Insert For pricing information visit www gelifesciences com orderonline Related Products Refer To Immobiline DryStrip Gels page 269 Ettan IPGphor 3 IEF System page 272 IPGbo
120. re separated ona 12 polyacrylamide SDS gel run on miniVE Proteins were transferred to Hybond P membrane using miniVE Blot Module GroEL was detected using an anti GroEL antibody ECL Plus Western Blotting Detection System and Hyperfilm ECL Two fold serial dilutions of a crude E coli protein extract and samples of purified recombinant green fluorescent protein were separated on q 12 polyacrylamide SDS gel run on miniVE Gel was stained with Coomassie Blue GE Healthcare SDS PAGE IEF Mini Vertical Units SE 250 and SE 260 SE 250 left and SE 260 right Mini Vertical Units provide fast electrophoresis results in less than 45 min e Premium multi option mini vertical electrophoresis unit e Choose the SE 250 for fast results using a 10 x 8 cm mini gel format or the SE 260 for higher resolution using a 10 x 10 5 cm gel format e Run two gels at once in a single unit and rely on the efficient cooling system to deliver flat side by side sample lanes with no smiling e Assemble gel sandwiches and electrophoresis units quickly and easily using T shaped spacers and specially designed clamps with integral springs e Cast up to four gels at once using a variety of casting stands or use precast gels from other manufacturers e Trust the safety lid interlocks to prevent accidental operator contact SE 260 Mini Vertical Unit offers the combined advantages of small format and efficient cooling an ideal combination for
121. rforming the IEF step of 2 D electrophoresis The use of IPG gels delivers high resolution 1 and improved interlaboratory reproducibility 2 3 compared with classical 2 D electrophoresis using carrier ampholytes Immobiline DryStrip gels are available in a variety of overlapping pH ranges seven narrow five medium and three broad and five lengths The narrow pH range 1 pH unit 24 cm IPG strips offer the highest resolution and loading capacity available see page 269 Two instrument choices for performing first dimension IEF Both the new Ettan IPGphor 3 Isoelectric Focusing System and the Multiphor II Flatbed Electrophoresis Unit are designed to handle simultaneously as many as 12 IPG strips up to 24 cm long Ettan IPGphor 3 uses innovative strip holders regular and cup loading and offers a fast and simple solution for IEF Ettan IPGphor 3 is a dedicated instrument for Immobiline DryStrip gels The combination of high voltage efficient cooling and ceramic strip holders can reduce IPG strip focusing time to a few hours Ettan IPGphor 3 accommodates IPG strip lengths of 7 11 13 18 and 24 cm see page 272 Multiphor II provides a flexible and economical alternative for performing first dimension IEF When equipped with Immobiline DryStrip Kit which features movable electrodes Multiphor II can handle essentially any length IPG strip IEF run times are longer on Multiphor Il as compared with Ettan IPGphor 3 but Multiphor II
122. ryer tnp pH 3 10 NL 24 cm 12 17 6002 45 mes Immobiline DruStrip pH 3 10 24 cm 12 17 6002 44 a e Barcoded strips for convenient sample and batch tracking _ f O Immobiline DruStrip gels 18 cm Immobiline DruStrip gels are available in fourteen overlapping Immobiline DryStrip pH 3 5 6 NL 18 cm 12 17 6003 56 O pH ranges and five strip lengths 7 11 13 18 and 24 cm and Immobiline Drystrip pH 5 3 6 5 18 cm a gt y represent the most comprehensive selection of narrow non ine 7 Pir S T ar18 eM I e O medium and wide range IPG strips available see Ordering A a a P cal _ Immobiline DruStrip pH 3 11 NL 18 cm 12 17 6003 76 OD Information The five narrow pH range 1 pH unit 24 cm IPG ee ee Sei ey i I yStrip pH 3 5 4 5 18 cm 12 17 6001 83 n strips deliver the highest resolution and protein loading Immobiline DryStrip pH 4 0 5 0 18 cm 5 capacity available which results in well separated spots with Immobiline DryStrip pH 4 5 5 5 18 cm 12 17 6001 85 N high protein content after the second dimension separation Immobiline DryStrip pH 5 0 6 0 18 cm 12 17 6001 86 The pH 3 11 NL strip covers most proteins found in prokaryotic E E Pa aaa f i uStrip pH 4 7 18 cm 12 17 1233 01 and eukaryotic cells while the narrow range strips can be used Irmmobiline Drustrip pH 6 9 18 cm 12 17 6001 88 when higher resolution is required The pH gradients at the Immobiline DryStrip pH 6 11 18 cm 12 17 6001 97 extreme ends of the pH s
123. s Normalization of the internal standard across gels allows the ratio of relative abundance of the same protein across gels to be compared directly separating gel to gel variation from biological variation Even small differences in expression levels can then be determined by comparing the ratio obtained from one fluorescently labeled sample directly with another As a result it is possible to see 10 differences in protein abundance between samples with greater than 95 statistical confidence GE Healthcare 2 D Electrophoresis 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE Introduction to 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE using Ettan DIGE System continued Experimental design for CyDye DIGE Fluor minimal dyes An example of a recommended experimental design to derive statistical data on any differences between control and treated samples labeled with CyDye DIGE Fluor minimal dyes Cy2 Cy3 or Cy5 is outlined below Six control and six treated samples are examined each sample having its own internal standard according to the table a pool is created by adding equal amounts of each sample together Gel number Cy2 Cy3 Cy5 1 Pooled standard Control 1 Treated 3 2 Pooled standard Control 2 Treated 2 3 Pooled standard Control 3 Treated 1 4 Pooled standard Treated 6 Control 4 5 Pooled standard Treated 5 Control 5 6 Pooled standard Treated 4 Control 6 Experimental variation is very low with Et
124. s Software v6 5 page 265 DeCyser IDA AJga Me Edt Took bisp fs apanan Expres A Arap er Prove og n ie hapit E Tem deals oes rer krh i Teli pr a i E FA Ta al EM mpy Finer Set sz len ujal Priben bebe EA map palega 5 Tube O O Results from Pattern Analysis calculations with Hierarchical Cluster Analysis tab shown GE Healthcare 2 D Electrophoresis 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE DeCyder MS Differential Analysis Software MONO mol ptes O pies PeryOeteca DeC p er S E Example of results from the PepMatch module e Provides a completely new level of software support to MS based differential analysis without the need for any mass tag chemistry e Reduces hands on time and minimizes user to user variation by automatic detection matching and analysis of peptides from multiple LC MS raw data files e Automatically presents quantitative information and matching statistics from complex experiments such as time dose series e Visualizes LC MS data in a novel way to enable effective navigation and presentation of large data sets DeCyder MS Differential Analysis Software significantly increases analysis efficiency by automating measurements of peptides from large data sets with statistical confidence This proprietary software based on DeCuder 2 D Differential Analysis Software automatically detects matches and analyzes peptid
125. s com orderonline Store dark at room temperature GE Healthcare Electrophoresis Standards and Reagents Urea Urea solubilizes and unfolds most proteins to their fully random conformation with all ionizable groups exposed to solution Urea is included in the 2 D sample solution at a concentration of at least 8 M Ettan Chemicals e Convenient and reliable designed specifically for use with Ettan Spot Handling Workstation e Guaranteed high and uniform reagent quality e Formats adapted for batch processing With the large number of stages during the processing of 2 D gels from the gel to protein identification the quality and uniformity of the reagents used is paramount At each stage of spot picking digestion spotting and MALDI ToF analysis the quality of the chemicals used is essential to produce an accurate and reliable result Ettan Chemicals have been designed to minimize the variations within runs and between runs that can be caused by the processing chemicals The chemicals are supplied in convenient batch aliquots to minimize oxidation and contamination of stock bottles Trifluoroacetic Acid TFA TFA has two functions when used with Ettan Spot Handling Workstation The pH reduction caused by TFA assists in the extraction of the digest from the gel plugs in the Digester TFA is also used to dilute the ACH Cinnamic Acid mixture where it functions as an acidifier to maintain protonation in the matrix The purit
126. sOne Glucerol 87 For pricing information visit www gelifesciences com orderonline Store at room temperature GE Healthcare Electrophoresis Standards and Reagents PlusOne Glycine PlusOne Glycine Glycine is frequently used as a component of electrophoresis buffers especially in the Laemmli discontinuous buffer system for SDS PAGE Immobiline DryStrip Cover Fluid This specially purified fraction of mineral oil protects Immobiline DryStrip gels from drying and from the atmosphere during the first dimension of 2 D electrophoresis The fluid is also suitable as a contact between cooling plate and gel backing film in horizontal electrophoresis lodoacetamide Ultrapure proteomic grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples PlusOne Mercaptoethanol 2 Mercaptoethanol is used to reduce disulfide bonds for electrophoretic analysis of protein At low concentrations mercaptoethanol prevents the oxidation of proteins TECHNICAL SPECIFICATIONS M 78 13 Minimum assay 99 Weight per ml qt 20 C 1 12 g PlusOne N N Methulenebisacrulamide PlusOne N N Methulenebisacrulamide is a general cross linker used with acrylamide for PAGE techniques It is available as a dry powder and a premixed solution TECHNICAL SPECIFICATIONS M 154 17 gt 99 9 lt 0 02 Minimum assay Acrylic acid HPLC GE Healthcare
127. session and the location of each picked protein in its respective microplate well is automatically logged to facilitate sample tracking When picking a limited number of protein spots or for pooling of samples the software also includes a click and pick function whereby proteins can be picked without an ImageMaster generated picklist Ettan Spot Picker is compatible with large format DALT precast gels self cast gels and nonbacked gels Gels cast on backing provide stable gel dimensions during spot picking During the picking process gels are covered by liquid to eliminate gel drying Nonbacked gels are also easily handled using the Ettan Spot Picker Nonbacked Gel Kit Ettan Spot Picker is supplied with 1 4 mm and 2 0 mm picker heads reference markers gel holders gel tray and microplate racks A Protective Hood which minimizes risk of gel contamination is optional GE Healthcare Automated Gel Handling Supported by OGE approved ORDERING INFORMATION Product Quantity Code Number Ettan Spot Picker 1 18 1145 28 Including computer and monitor Accessories Target Layout Upgrade Software 1 11 0002 94 Picker Head 1 4 mm for 1 mm thick gels 1 18 1147 03 Picker Head 1 4 mm for 1 5 mm thick gels 1 18 1147 05 Picker Head 2 0 mm for 1 mm thick gels 1 18 1143 64 Picker Head 2 0 mm for 1 5 mm thick gels 1 18 1143 37 Reference Markers 560 sheet 2 18 1143 34 Gel Holder 2 18 1143 50 Gel Tray 1 18 1143 38 Glass
128. tan DIGE system and we therefore recommend biological replicates and not replicates of the same sample If the same experiment is carried out using conventional 2 D electrophoresis using one sample per gel at least 12 gels would be required To gain any meaningful statistical data using this approach would require a minimum of three replicates of each making 36 gels in total GE Healthcare Experimental design for CyDye DIGE Fluor saturation dyes from the Scarce Sample Labeling Kit An example of a recommended experimental design to derive Statistical data on any differences between control and treated samples labeled with CyDye DIGE Fluor saturation dyes Cy3 or Cy5 is outlined here Six control and six treated samples are examined each sample having its own internal standard Gel number Cy3 Cy5 1 Pooled standard Control 1 2 Pooled standard Control 2 3 Pooled standard Control 3 4 Pooled standard Control 4 5 Pooled standard Control 5 6 Pooled standard Control 6 7 Pooled standard Treated 1 8 Pooled standard Treated 2 9 Pooled standard Treated 3 10 Pooled standard Treated 4 11 Pooled standard Treated 5 12 Pooled standard Treated 6 For more information visit www gelifesciences com udo1125 3 O me OD T 263 n Q O O O i jad Q O LLJ 264 2 D Electrophoresis 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE CyDye DIGE Fluors e Allow detection of up to three prelabeled pro
129. tances 15 T 2 7 C polyacrylamide gel l HMW SDS A 10 ul aliquot of a two fold dilution of HMW SDS Marker Kit was e Bands can be visualized by using Deep Purple Total Protein O separated on 7 5 T 2 7 C polyacrylamide gel Stain Coomassie Brilliant Blue or silver staining HMW Native A 10 ul aliquot of a two fold dilution of HMW Marker Kit was separated on 5 12 5 polyacrylamide gradient gel All gels were stained with gt PhastGel Blue R Numbers represent molecular weights M in thousands O D O Protein M Source Reference Amount ug T Peptide Marker Kit 2512 16949 Horse myoglobin peptides 16949 equine 14404 equine 10700 equine 8159 equine 6214 equine 2512 equine HMW SDS 53000 220000 Myosin 220000 rabbit muscle 1 25 a 2 Macroglobulin 170000 bovine plasma 2 100 B Galactosidase 116000 E coli 3 16 Transferrin 76000 human 4 17 Glutamate dehydrogenase 53000 bovine liver 5 18 LMW 14000 97000 Phosphorylase b 97000 rabbit muscle 6 67 Albumin 66000 bovine serum 7 83 Ovalbumin 45000 chicken egg white 8 147 Carbonic anhydrase 30000 bovine erythrocyte 9 83 Trypsin inhibitor 20100 soybean 10 80 a Lactalbumin 14400 bovine milk 11 116 HMW Native 66000 669 000 Thyroglobulin 669000 porcine thyroid l2 76 Ferritin 440000 equine spleen 13 50 Catalase 232000 bovine liver 14 36 Lactate dehydrogenase 140000 bovine heart 15 48 Albumin 66000 bovine serum 7 40 References for technical specifications 1 Starr R
130. tein samples and standards on the same 2 D electrophoresis gel e Size and charge matched dyes enable co migration of labeled samples within the gel e Bright and highly sensitive dyes allow the use of the minimal labeling technique e Minimal loss of signal during labeling separation and scanning e No change in signal over wide pH range used during first dimension IEF separation e Discrete signal from each fluor with minimal cross talk contributes to high accuracy CyDye DIGE fluors are available as minimal and saturation labeling dyes The minimal dyes are intended for general 2 D application use where sufficient amounts of sample are available The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available for example in Laser Capture Microdissection In total three minimal dyes and two saturation dyes are available CyDye DIGE fluors are exceptional dyes for multicolor analysis offering bright and intense colors with narrow excitation and emission bands The fluors are spectrally distinct making them ideal for multicolor detection CyDye DIGE fluors utilize these benefits but are also size and charge matched specifically for 2 D DIGE using Ettan DIGE system CyDye DIGE Fluor Minimal Dyes Protein samples and the internal standard are each labeled with one CyDye DIGE Fluor minimal dye These labeled samples are then combined run on an iso
131. the majority of cysteine groups in a protein from a sample being labeled For this reason the method has been called saturation labeling The CyDye DIGE Fluor saturation dyes covalently label cysteine residues They have a net neutral charge and therefore do not alter the charge on a protein after labeling They are also matched for molecular weight ensuring that the same protein from a standard and test sample labeled with the different dyes will overlay when separated on a single gel GE Healthcare 2 D Electrophoresis 2 D Fluorescence Difference Gel Electrophoresis 2 D DIGE Typhoon For main product entry see page 376 Ettan DIGE Imager For main product entry see page 383 DeCuder 2 D Differential Analysis Software v alaia BeCyder 2D Biological Variation Analysis Differentiallln get Analysis y r r Image Lender Bazh Pracecose XML Toolbox aSo AF q DeCuder 2 D v6 5 startup screen showing analysis workflow e Dedicated software for 2 D DIGE analysis e Automated analysis significantly reducing hands on time e Internal standard approach increasing accuracy and simplifying gel to gel matching e No spot matching within gels eliminating matching errors e Automatic Oracle client and server installation offering easy project handling and data security e Optional DeCyder Extended Data Analysis EDA a multivariate statistics module for easier and faster biomarker discovery
132. ting Markers 1 pack 25 loadings RPN810 e Tagged recombinant protein markers allow accurate For pricing information visit www gelifesciences com orderonline calculation of molecular weight of target protein s Contains ECL DualVue Markers 125 ul and S protein HRP 50 ul e Product is compatible with any HRP substrate as well as both PVDF and nitrocellulose membrane types Related Products A Nm RYU ECL Western Blotting Detection Reagents RPN2106 page 357 Amersham ECL DualVue Western Blotting Markers perform two ECL Plus Western Blotting Detection Reagents RPN2132 page 356 key functions First the prestained marker proteins confirm ECL Advance Western Blotting Detection Kit RPN2135 page 355 protein transfer in addition to clearly defining blot orientation Amersham ECL HRP Linked Secondary Antibodies page 360 Second the recombinant tagged proteins are detected on film palate U s poge sie or by CCD imaging in conjunction with the target protein s of interest enabling highly accurate molecular mass m determination of the target protein s The tagged markers are detected easily and specifically by means of a specific hi conjugate that eliminates any cross contamination between or target protein s and markers O Amersham ECL DualVue Western Blotting Markers contain a O mixture of three prestained colored markers of defined molecular weight from 15000 to 100000 and seven tagged O recombinant proteins molecular weights
133. tion tool 1 80 6127 88 Slotted silicone rubber gaskets for upper buffer chamber 2 80 6174 43 Laminated silicone rubber gaskets for casting stand 2 80 6174 62 Buffer dam 1 80 6175 19 Upper buffer chamber for SE600 SE600 Ruby and SE 660 1 80 6176 33 Lid with power cables for SE 600 Ruby 1 80 6476 34 Lid with power cables for SE 660 1 80 6176 52 High voltage safety lead set 1 80 6177 09 Lower buffer chamber for SE 600 Ruby 1 80 6476 15 Heat exchanger for SE 660 1 80 6183 74 Lower buffer chamber for SE 660 1 80 6191 15 N Gel Seal 1 80 6421 43 ET Spirit level 1 80 6194 19 Clamps and Cams L Clamp assemblies 16 cm 2 80 6173 29 O Clamp assemblies 8 cm 2 80 6187 35 K a SE 600 Ruby Standard Vertical Unit Components Clamp and cam kit four 16 cm clamps and 8 black cams 1 80 6173 67 A Lid assembly for SE 600 Ruby G Spacer various Cams black for new style clamps with cam holes 4 80 6174 24 80 6476 34 H Clamp 16 cm 80 6173 29 ar O Lid assembly for SE 660 I Glass plate 18 x 16 cm 80 6178 99 For pricing information visit www gelifesciences com orderonline Phar 80 6176 52 is not shown J Casting stand 80 6175 00 O B Lower buffer chamber for SE 600 K Heat exchanger 80 6183 74 Rubu 80 6476 15 L Level 80 6194 19 Lower buffer chamber for SE 660 M Wonder Wedge separator LL 80 6191 15 is not shown 80 6127 88 C Upper buffer chamber 80 6176 33 N Spacer Mate 80 6181 65 D Gel seal 80 6421 43 O Buffer dam 80 6175 19
134. trolled parameters include temperature voltage current power and volt hours TECHNICAL SPECIFICATIONS Separation and Control Unit Capacity 1 or 2 gels Keyboard 31 keys with audible tone Display 40 digit alphanumeric liquid crystal LEDs 4 LEDs for status information Programmable parameters Voltage current power temperature duration in volt hours sample application and an extra alarm for 9 programs of 9 steps Voltage range 10 2000 V Current range 0 1 50 0 mA Power range 0 1 7 0 W Volt hour integrator range 1 9999 Vh step Separation temperature range 0 C to 70 C Alarm Audible alarm at end of separation Dimensions W x D x H 46 x 30 x 14 cm Weight 6 2 kg Lithium battery Short circuit protection protected against power disturbances and static discharges lid power interlock and alarm CE 89 336 EEC EMC directive CE 73 23 EEC LV directive EN 61010 1 IEC1010 1 UL3101 1 CSA22 2 1010 1 Battery back up Safety features Safety certifications Supported by ORDERING INFORMATION Product Quantity Code Number PhastSystem Separation Control and Development Units 1 18 1018 23 120 VAC PhastSystem Separation Control and Development Units 1 18 1018 24 220 VAC Separation Control Unit 120 VAC 1 18 1200 00 Separation Control Unit 220 VAC 1 18 1200 10 Accessories PhastGel Sample Applicators 12 x 0 3 ul 50 18 1614 01 PhastGel Sample Applicators 8 x 0 5 pl 50 18 1617 01 Ph
135. utomatically mix and spot digested proteins and matrix onto any MALDI MS targets e Easy to set up and run acts directly on Ettan Digester output files e Special needle design with hydrophobic coating to eliminate cross contamination e Automatically spots 384 samples batch in less than 3 h Ettan Spotter is designed to reproducibly spot protein samples and matrix on multiple MALDI MS targets for subsequent analysis by mass spectrometry Samples are presented to the Ettan Spotter in 96 well microplates The instrument acts directly on Ettan Digester output files which provides automated sample tracking from spot picking through to digestion and completion of spotting The easy to use software allows the selection of default or customizable spotting methods Ettan Spotter uses a single needle for spotting which is overlaid with a hydrophobic coating for eliminating cross contamination between samples as well as any carryover of liquid The needle is automatically washed after spotting a sample There is no need for a desalting step prior to spotting since salt levels are controlled during the digestion methods used in Ettan Digester The software enables the sample to be spotted twice on the MALDI target in order to allow sample concentration Up to 384 samples can be spotted in automated mode Throughput is determined by the number of microplates used up to four microplates can be used with digested material that can be fitted onto th
136. w capabilities to optimize chromatography and MS MS settings In addition where MS MS data is available the module provides the researcher with an option of submitting all or a selected subset of peptides for protein identification The Batch Processor enables the PepDetect module to process several hundred LC MS raw data files without user interaction PepMatch module Peptides from different runs are aligned using this module PepMatch detects small quantitative differences between peptides across multiple runs with high statistical confidence Various normalization techniques can be applied to further improve results The module supports a wide range of experimental designs such as control treated experiments as well as time dose studies In addition when MS MS data is available deviating peptides can be submitted for protein identification and once identification information is available this can also be used to look at co variation of peptides from the same protein Automated Statistical Analysis DeCyder MS Differential Analysis Software enables internal standardization of protein abundance differences The software features fully automated statistical analysis and a list of statistically significant differences is generated automatically For more information visit www gelifesciences com udo1125 3 O me OD T 267 n Q O S O O Q LLJ 268 2 D Electrophoresis 2 D Fluoresc
137. ww gelifesciences com orderonline Related Products Code Number Refer To Ettan Spot Picker 18 1145 28 page 279 Ettan Spotter page 282 TECHNICAL SPECIFICATIONS Sample source Polyacrylamide gel plugs in microplates Sample handling 8 samples simultaneously Detection system compatibility Silver Coomassie or fluorescence Digestion capacity 384 samples per batch Process capacity 384 samples in less than 8 h digestion 37 C 2 h Coomassie SYPRO stained gel 100 fmol of phosphorylase B or BSA in an SDS gel plug could be identified by loading 10 of the resulting digested peptide mix onto the MALDI ToF target 18 C to 30 C 65 F to 86 F 80 noncondensing 75 105 kPa up to 2000 m altitude 100 120 V or 220 240 V 10 50 60Hz single phase Mains fuse Digester MultiValve 1x T2 A 110 V or 2 x T2 A 220 V Sensitivitu Operating temperature Maximum relative humidity Air pressure Mains voltage Power consumption Max 200 VA Safety UL 1260 CSA C222 No 151 IEC 1010 1 EMC EN 50082 1 FCC Class A EMI EN 50081 1 Dimensions W x H x D 91 x 56x 61 cm Weight 53 kg For more information visit www gelifesciences com ydoj 9a 3 O x O T 281 n w O O i mur Q LL 282 2 D Electrophoresis Automated Gel Handling Ettan Spotter Ettan Spotter automatically spots protein samples and matrix onto MALDI MS targets e Robotic instrument designed to a
138. x is used together with the IPGbox Kit to rehydrate 7 to 24 cm Immobiline DryStrip gels The IPGbox Kit contains 10 disposable Reswell Trays and one disposable IPGbox Insert The IPGbox comes with the complete set of accessories to rehydrate 10 full rounds of 12 IPG strips each For more information visit www gelifesciences com udo1125 3 O me OD T 2 1 n O O O i mur Q LLJ 272 2 D Electrophoresis First Dimension Ettan IPGphor 3 IEF System Ettan IPGphor 3 IEF System is easy to use capable of generating fast reproducible runs and is designed to work as stand alone or in conjunction with PC control e Ettan IPGphor 3 IEF System is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2 D electrophoresis e Run upto 12 IPG strips 7 11 13 18 or 24 cm simultaneously using Ettan IPGphor Manifold Ettan IPGphor Manifold Light or ceramic strip holders e Use sample cups with Ettan IPGphor Manifold or Ettan IPGphor Manifold Light to significantly increase protein loads e Ettan IPGphor 3 Control Software allows management of up to four Ettan IPGphor 3 IEF units simultaneously each running a different set of run parameters e Lid design allows optimum running with light sensitive protein samples e The system is fully compatible with the earlier Ettan IPGphor IEF unit and is ideal for 2 D DIGE applications Th
139. y for 100 loadings using 1 ul per loading after mixing with tracking dye e Consists of 11 easily identifiable bands 0 5 1 0 1 5 2 0 2 5 3 0 4 0 5 0 6 0 8 0 and 10 0 kb when run on a 0 8 agarose gel e Bands stain with approximately the same intensity except jaa DAM aderana ourDNi for the 1 0 kb band which is three times the intensity of the samples were analyzed on a 0 8 agarose other bands gel with TAE containing ethidium bromide e Not designed for quantitating DNA content in a sample as running buffer Bands were visualized under UV light Lane 1 0 5 ug KiloBase DNA Marker lane 2 100 ng Kan GenBlock 1282 bp lane 3 100 ng pUC18 Smal BAP 2686 bp 27 4860 01 lane 4 100 ng pGEX 4T 3 4900 bp 27 4583 01 lane 5 100 ng pMSG CAT 8405 bp 27 4505 01 lane 6 0 5 ug KiloBase DNA Marker Restriction Digests of Naturally Occurring DNAs e For sizing DNA fragments from 72 to 23130 bp on agarose gels Product Quantity Code Number e Contains either DNA oX 174 RF DNA or a combination of DRigest III DNA Hind IIl X 174 RF DNA Hae III Digest 25g 27 4060 01 the two substrates which have been digested with restriction oX 174 RF DNA Hae III Digest 100g 27 4044 02 enzymes For pricing information visit www gelifesciences com orderonline e DRigest III 27 4060 01 is supplied in a convenient dried form eee re ee to allow the user to adjust the concentration a e a eee A complete list of
140. y of Ettan Chemicals TFA is optimized with regard to the demands of MALDI ToF analusis The contents of each bottle are diluted before use GE Healthcare DIGE approved ORDERING INFORMATION Product QuqntitU Code Number Urea 500g 17 1319 01 For pricing information visit www gelifesciences com orderonline ORDERING INFORMATION Product Trifluoroacetic Acid 50 ml Trypsin sequencing grade 10 x 250 ug Quantity Code Number 1 17 6002 76 1 17 6002 75 For pricing information visit www gelifesciences com orderonline TECHNICAL SPECIFICATIONS Trypsin M 24000 Specific activity 5000 units mg protein Trifluoracetic acid M 11505 Trypsin sequencing grade Trypsin specifically hydrolyzes peptide bonds at the carboxylic sites of lysine and arginine residues A high purity trypsin preparation is essential to achieve good protein identification results Trypsin sequencing grade is purified from porcine pancreas which has been proven to be superior for protein sequencing compared to trypsin derived from other sources The reagent has been further treated with TPCK L 1 chloro 3 4 tosyl amido 4 phenyl 2 butanone which irreversibly inhibits chymotrypsin and many other serine and cysteine proteases such as bromelain ficin and papain A further purification by affinity chromatography removes pseudotrypsin and results in a highly active and stable preparation Trypsin sequencing grade is modifie

IEF, SDS-PAGE & 2–D Electrophoresis

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IEF, SDS-PAGE &amp; 2&ndash;D Electrophoresis

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IEF, SDS-PAGE & 2–D Electrophoresis