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ArmedXpert™ User`s Manual

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1. 1 222 25 9 12 22 22 2 24 25 9 12 22 22 2 24 2 14 15 17 X Y 9 12 22 22 2 24 25 1 1 0851179 021511 D7S820 5 D3s1358 THO1 D13S317 D16s539 D25 1338 D195433 vWA TPOX D18S51 Amel D5 8 9 10 FALSE 24 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 8 9 15 16 17 9 10 1 12 11 12 13 6 7 7 9 10 8 9 8 9 10 24 24 2 6 7 8 6 7 8 9 12 13 45 8 9 10 5 89 15 16 17 9 10 11 12 11 12 13 6 7 8 9 10 24 242 6 7 8 6 7 8 9 12 13 45 8 9 10 5 8 9 15 16 17 9 10 11 12 11 12 13 6 7 7 8 9 17 18 19 20 912 18 912 13 1925 14182 15 18 12 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 8 1 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18215 17 18 19 8 11 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 182115 17 18 19 B 11 Ianue 3 m g Im 22 26 pee 22 esum 9 EE ae mmn 12 13 28 29 30 9 10 10 11 12 15 16 6 93 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 x 22 22 2 24 2 12 2931 912 910 14 793 1214 912 17520 1315 1416 81 1618 XY 19 23 FIGURE 3 14 Column widths are changed to
2. Select a reference to apply Apply Reference Select a reference to apply Apply Reference Select a reference to apply z Apply Reference Select areference to apply z Apply Reference Select a reference to apply Apply Reference Select a reference to apply Apply Reference Auto call references z List foreign FIGURE 7 7 Reference Selection Box All selected references will be highlighted blue on the sample table The reference s will also be indicated as such i e Ref 1 Ref 2 in the Mixture Interpretation area of the Mixture Interpretation Window NOTE Only combinations including the references will be shown in the Mixture information area Click on the Auto call references button to automatically fill in the Reference 1 as Profile 1 and Reference 2 as Profile 2 This feature will allow you to only have to select a call for the unreferenced profile s Note The Auto call Reference feature is only auto populating the profile with the chosen reference It is not taking into account any calculations or making any kind of interpretation The List Foreign option will list all alleles that are foreign to the reference on the View Call Report Click on this to enable it before you click on Auto call references button 68 ARMEDXPERT USER S MANUAL Views Data QC Checks Match amp Comparison Interpretation Reporting VY Highlight 4 amp S amp Y
3. RFLl s RFU s RFL s Base Pairs Base Pairs Base Pairs Base Pairs Base Pairs Base Pairs ocu Locus Locus Locus 4 31 Allele s Allele s Allele s Allele s Allele s Allele s RFUl s RFU s RFL s RFUf s RFL s RFLl s Base Pairs Base Pairs Base Pairs Base Pairs Base Pairs Base Pairs LOCUS amp Allele s Allele s Allele s Allele s Add Locus RFU s RFU s RFUl s RFU l s Base Pairs Base Pairs Base Pairs Base Pairs FIGURE 4 4 New Sample Window 42 ARMEDXPERT USER S MANUAL To edit any sample on your sample table click the Edit Sample button The select pick via mouse window will appear as seen below in figure 4 5 You can use your mouse to select the sample you wish to edit or click on the VW arrow and select the one you would like to edit from the drop down list Views Data QCcChecks Match amp Comparison Interpretation Reporting a y Ss 28 3 Change Combine Commit Samples Load Samples Connect Disconnect Sort Kit Sources toDataSource From Data Source Server Pick via mouse FIGURE 45 Select Pick via mouse Window A window similar to the Enter new sample window will appear At each locus the allele s RFU s and Base Pairs can be added to or changed All fields that cannot be changed are grayed out Once you complete your edits click on the Edit button c3 s 3 Set Sample Source Type identifiler Send To 5
4. Begin Mixture Single Likelihood Probability Mixture Interpretation Source Ratio of Inclusion Mixture Interpretation Frequency Calculations DEMO 2 Component 4 Vict Mix Stains Analyst txt Mixture Interpretation DDA Interpretation ob LLLI Setup 8 9 10 24 24 2 6 7 8 6 7 8 Contibutorit2 7 43 28 10 101 Pick via mouse 0156 1 935 9333 1 15 2F1 Shirt Vict 06 06 20 D01 Operations 7 99XX999901 15 2F1 Shirt Vict 06 06 20 01 12 13 28 29 30 9 10 110 11 in 12 13 28 29 30 29 30 9 10 10 11 i s c 13 10 10 16 Locus D21911 3 7 gt Highestto Profile 03 Victim 29 30 P Avg PH 0 00 5 Relerence 12 13 28 29 30 9 10 10 11 R7 a rt ERU eu E 13 14 28 311 2 12 10 41 28 29 30 P 0 00 poem M E RFUs 318 1144 994 Lock locus y 0157 99 9999K1 11 Blooc BPs 200 204 208 i li Popout 12 13 28 29 30 10 11 E cals C R12 29 31 9 12 910 Select a reference to apply Apply Reference Select a reference to apply Apply Reference ge 1144 s94 ly m Stutter Add Profle v y Select a reference to apply 7 Apply Reference Select a reference to apply 7 Apply Reference Auto callreferences 7 V List foreign For a 2 contributor 3 allele mixture of types AA amp
5. References ls oni ee tiie 1213 PROS 5 li Foerrerve Ateler E h Profile 2 na y mee 150 Ww 1 sus 5 L 5 8 Locus DESTIVS2I BP 13955 144 25 Popout emcatrenat Gow Selection i 1 For a 2 contributor 2 allele mixture of types AA amp AB 2 2 combination s 11 051 196 Ret 1 12 p 1 IF 181 Ref 1 13 0 19 2 For a 2 contributor 2 allete mixture of types AB amp AB 1 1 combination s 12 13 p 67 Ret 1 12 13 phr 0 87 Ret 1 FIGURE 2 28 Mixture Interpretation Imported Click on OSIRIS Plot file to import in an OSIRIS plot file When you select this option the OSIRIS Plot window will open as shown below in Figure 2 29 Click the Add button to add the OSIRIS plt files Add them in the order you want them to appear in the final report Double click on a listed plt file to edit the name Click on any of the OSIRIS plt files listed and click the Remove button to remove them from the list Once finished with adding click the Plot button OSIRIS Plot c3 5 2s Add OSIRIS plt files in the order that you want them to appear in the final report Double click a listed plt file to edit the name that appears on the report Plot file 9 Add Remove Plot Print LI p Down FIGURE 2 29 OSIRIS Plot Window 27 ARMEDXPERT USER S MANUAL Export The Export command allows you to export your Page
6. As shown In Figures 7 12 and 7 13 the RFU amount under loci 12 was refigured from 1580 to 1392 once stutter was considered and the amount is now highlighted in pink to indicate stutter was applied This also changed the calculations in the Mixture Information section If the Apply Globally option is checked it will apply stutter to all applicable alleles at each Locus ae 100 Sz 1580 2345 Profile 1 Y Apply Globally Appl Stutter_ 0 08 Mixture Information Only combinations including the following reference profiles are included 12 13 All combinations have PHr gt 0 3 mPH 175 mP gt 0 1 For a 2 contributor 2 allele mixture of types AA amp AB 2 2 combination s 12 13 phr 0 60 p 0 96 Ref 1 12 12 p 0 04 12 13tphr 1 00 p 0 81 Ref 1 13 13 p 0 19 2 For a 2 contributor 2 allele mixture of types AB AB 1 1 combination s 12 13 phr 0 67 Ref 1 12 13 phr 0 67 Ref 1 FIGURE 7 12 Mixture Information before stutter applied As shown below in Figure 7 13 once Stutter has been applied the Apply Stutter button changes to Remove Stutter Also in the Mixture Information portion of the window a note has been made to indicate that the RFU s have been recalculated for stutter To remove Stutter to a specific locus click the Remove Stutter button If you want to turn the Apply Globally feature off click the box left of it and the check mark will be removed
7. 14 1 13 0 Ladder Identifiler 06 06 20 A03 4 5 9 10 11 12 14 1 13 0 Ladder Identifiler 06 06 20 F03 14 1 13 0 XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 H02 16 16 0 0 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 001 16 4 12 0 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 E01 16 4 12 0 XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 FO1 13 14 15 l 16 4 12 0 XX0161 1 QAO03F1 06 06 20 H01 1 12 10 1418 6 89 11 13 1724 1314 1417 4 1 3 0 ee py ERE DR XX0162 1 Reag BlankF1 06 06 20 B02 XX0162 2 Reag BlankF2 06 06 20 C02 XX0164 99XX9999Q1 2 LFS Vict 06 06 20 D02 XX0165 QA004 QA 06 06 20 E02 9 12 910 14 7 9 3 12 14 ee ae a 416 16 18 2 1 0 XX0166 Reag Blank 06 06 20 F02 es 10 12 12 13 20 24 13 14 2 17 19 1435 X ae 2224 4 1 3 0 XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 XX0169 QA026 QA 06 06 20 B03 XX0170 Reag Blank 06 06 20 C03 XX0171 Pos Con 06 06 20 D03 XX0172 Neg Con 06 06 20 E03 8 13 2 2 0 0 a ee ee ee ees ee ee eee 8 93 11 11 22 1923 1415 1718 8 1519 11 2324 2 1 1 0 d ee ee es ee es ee ee ee eee Eee eee FIGURE 6 4 Find Where Reference is Included Example ARMEDXPERT USER S MANUAL Find Included in the Reference is used to find loci t
8. USER S MANUAL Or add a new connection name by clicking on the box next to the Connection Name The box will appear in white and you can type in a new name Login s Login Connection Properties 4b Connection Name Local PC v FIGURE 1 5 New Connection Name Change the connection type by clicking on the arrow next to the Connect Type box This will bring up a drop down list of available connection Select the appropriate Connection type Connection Name Local PC Connection Type SOL Server ig SQL Serv C n chess SQL Server Remoting onnection String SQLite FIGURE 1 6 Changing Connection Type Type in an appropriate Connection String The Connection String for SQLite connection can be changed to any user defined name Just highlight the current name shown and enter the new name It should read data source userdefinedname db The current name shown in the example below is ArmedXpett The db file is created in the same folder as the ArmedXpert application Once finished click on the Save this Configuration button If the Save this Configuration button is not clicked the connection configurations will be not used the next time you open the program Login c3 5 Login Connection Properties 4 Connection Name Local PC Connection Type SQLite x Connection String data sources rmedpert db Save this Configuration Delete this Configuration Ok Cancel J Options lt
9. 1 994999901 15 2F1 Shirt Vict 06 06 20 D01 Edit Lacus D851173 Locus D 21511 Locus D 75820 Locus Locus D 351358 Locus THOI Allele s 12 13 Allele s 28 23 30 Allele s 3 10 Allele s 10 11 12 Allele s 15 18 Allele s 5 3 3 RFU s 1580 2345 RFU s 916 1144 994 RFU s 594 2480 RFUl s 2001 609 1157 RFU s 1548 747 RFU s 1337 1465 Base Pairs 139 55 144 26 Base Pairs 200 36 204 36 20 Base Pairs 267 42 271 48 Base Pairs 321 95 326 12 33 Base Pairs 123 82 127 87 Base Pairs 171 18 186 17 Locus 5135317 Locus D165539 Locus 0251338 Locus D195433 Locus A Locus a O m LLL il Allele s 8 11 12 Allele s 3 12 13 Allele s 13 20 24 25 Allele s 13 14 14 2 18 2 Allele s 15 17 18 19 Allele s 8 11 RFU s 677 444 378 RFU s 409 1355 944 RFU s 456 1141 1053 3 RFU s 787 410 886 292 RFU s 332 934 423 903 RFU s 970 2010 Base Pairs 215 85 228 77 23 Base Pairs 268 4 280 49 284 Base Pairs 324 47 328 61 34 Base Pairs 117 36 121 33 12 Base Pairs 171 04 178 38 18 Base Pairs 223 34 241 83 Locus D13551 Locus Amel Locus D55816 Locus FGA l U Allele s 14 15 17 Allele s gt Y Allele s 3 12 Allele s 22 22 2 24 25 RFU s 1144 883 360 RFU s 1707 258 RFUl s 376 2105 RFU s 441 168 550 256 Base Pairs 290 93 295 08 30 Base Pairs 106 69 112 28 Ba
10. 13 14 14 amp 11 XX0162 1 Reag BlenkF1 06 06 20 002 ERE CE xx0162 2 Reag BlankF2 06 06 20 C02 suu XX0165 QA004 QA 06 06 20 E 14 7 93 00166 Reag Blank 06 06 20 F02 3X0170 T Blank 06 06 20 C03 XX0171 Pos Con 06 06 20 D03 XX0172 Neg Con 06 06 20 E03 Ladders Controls and Profiles are within established Jparaffeters FIGURE 5 3 Example of a Check Control screen Check Allele Ordering confirms that the alleles are all in correct order Any allele not in correct order will be highlighted pink M ArmedXpert C Program Files NicheVision Inc ArmedX pert data Saved_Profiles Page_Data Mix file complete Tb xls p i Views _ Data QC Checks Match amp Comparison Interpretation Reporting FWEZA 0 Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Alleles Count X multi Review Checks Standard Checks CODIS QC Qe Review Clear ID 2 GH LOng 1 1 1 009 1 ID 2 GH LOng RL4 1 005 1 ID 2 GH 1018 RL9 VL2 003 1 aus jee 1017 piir n5 us man 9 29 31 2 30 Suis us mms 93 83 eu ID 3 EG 20ng RL 12 1 1 006 1 1 628 BEN SEE 55 15 73 93 510 11 12 ID 3 EG 30ng 23 1 VL 1 010 111 PTI oT DEC DEEETENRD 3p 15161718 9 93 1112 10 EE ID Ladder 014 1 FIGURE 5 A Example of a Check Control screen 53 ARMEDXPERT USER S MANUAL Stutter checks for potential stutter It confirms that the
11. 1418 66 89 11 13 17 24 1314 1417 8 amp 11 121 RSS SPOT ee 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 119 20 24 25 13 14 14 2 18 215 17 18 19 8 11 114 15 29 31 9 12 9 10 1414 7 93 12 14 912 1720 1315 1416 181 161 DU MENSEM NM ECHTE NEM eee ERN 12 13 29 30 10 10 10 12 15 16 6 93 812 1213 2024 13142 1719 181 141 13 13 28 28 9 10 10 11 15 15 693 1 12 912 1925 14182 1518 11 11 141 12 15 30 30 11 12 11 11 1416 9 3 12 13 12 13 18 22 1215 1518 88 13 1 TERRIER NEN IEEE Es ees eee NET 13 13 30 30 10 111 10 12 1415 8 93 1 11 1 12 1923 1415 1718 88 151 ES a ESSET pecu eq RFUs are off scale above 6000 FIGURE 5 13 OSD Result Example ARMEDXPERT USER S MANUAL The PHR command evaluates peak height ratios to see that they meet the threshold The PHR settings are set up under the Quality Control Section in ArmedXpert Options Menu Any sample s locus that has a PHR imbalance is highlighted pink Any sample s locus that is not valid for this check is highlighted dark grey Qo gt ArmedXpert views Data QC Checks Match amp Comparison Interpretation Reporting amp y See SO a AZ a ERS wy Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Al
12. TPOX D18551 b Victim 12 13 29 30 10 1012 1516 amp 93 amp 12 12 13 20 24 12 142 17 19 amp 11 14 15 N Pick via mouse 0156 1 954X999901 15 2F1 Shit Vict 06 06 2001 ERT Highest to z3 L D351358 2 r7 Victim 15 16 P Avg 0 70 PHr 0 40 ocus 2 Le Lowest tt Suspect 15 P Avg 0 30 genere Alleles yy TER Wto i Alleles 15 16 Pelor men Eu XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 E L res mP 0 00 5 06 20 D01 RFUs 1548 747 Lock locus BPs 1238212787 tutte Suspect gj B Victim E Suspect 15 16 phr 1 00 p 0 65 Victim 1 15 p 15 16 phr 0 42 p 0 96 Ref 1 16 p 0 04 D8S1179 D3S1358 D19S433 For a 2 contributor 2 allele LLL MixInerp Proprtions FIGURE 7 24 cai report Example The Call Report includes additional worksheets to document each of your interpretation parameters and results locus by locus If you click on the Mix Interp Worksheet tab at the bottom of the report you will see a Mixture Interpretation Detail Report NOTE The report only shows the Loci that you have went to using the Mixture Interpretation Window Mixture Interpretation Details for XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 Only combinations including the following reference profiles are included 12 13 All combin
13. 0 8000 Average PHR 0 8031 0 8583 0 7260 0 8461 0 9295 wowv oi wpiv ol piv ol 0 9154 wDiv ol 0 7885 0 6404 0 4000 0 2000 Min 0 6719 07668 0 0000 0 5904 0 7211 98543 0 0000 0 0000 0 0000 0 8519 0 0000 0 6947 0 5204 0 8025 0 6647 0 6633 ages ae ao wagons A Mac 0 9716 0 9185 0 0000 0 9693 0 9539 1 0000 0 0000 0 0000 0 0000 0 9561 0 0000 0 8536 0 8348 0 9604 0 9604 0 9639 ESSEC ES RS SEC ESL std Error 0 0885 0 0465 pivot 0 1219 0 0678 0 0421 Div o wpiv o wpv or 0 0322 wprv or 0 0480 0 0981 0 0478 0 0854 0 0928 28552 lt jii 3 FIGURE 8 19 Example of a PHR Report Any Locus that does not contain data because there was only one allele at that Locus can be hidden from the report to make the report easier to be read hide a Locus click on the Locus title multiple Loci can be selected by holding down the Ctrl key and clicking on each Locus Then press the F5 button All selected Loci will be hidden from the report To redisplay the hidden Loci press the F6 button PHR Report D851179 21511 CSFIPO D351358 THO1 D195433 TPOX D18551 Amel D5S818 FGA 15Dilution oo OSO PHR Average 1 5 Dilution AveragePHR 0 9276 0 9028 0 9070 0 8630 0 8880 0 9247 0 9279 0 7175 0 9351 0 9492 0 9387 Std Deviation 0 0568 0 0611 0 0707 0 0770 0
14. 100 ESI 9 Default stutter 7 100 Appl e Globally Sort loci by allele count 0 08 FIGURE 7 9 mixture Interpretation Settings 69 ARMEDXPERT USER S MANUAL The mixture information area will only show combinations that are equal to or greater than the settings and include the reference profiles if applied The proportions p can be displayed as a dilution instead of a percentage To do this go to the Mixture Interpretation subsection under the Interpretation section in the ArmedXpert Options Window Click on the box next to List proportions as dilutions 1 X This can be turned on and off while in the Mixture Interpretation Window Only combinations including the following reference profiles are included 12 13 _ All combinations have PHr gt 0 3 MPH gt 175 mP gt 0 1 For a 2 contributor 2 allele mixture of types AA amp AB 2 2 combination s fi 3 i ph r5 B6 B EI Y QE jJ R ef 1 B 2 1 2 D Q4 12 13 phr 1 00 p 0 81 Ref 1 13 13 p 2 0 18 2 For a 2 contributor 2 allele mixture of types AB amp AB 1 1 combination s 12 1 S phr 0 6 Ref 1 12 TS phr 0 67 Ref 1 FIGURE 7 10 Mixture Information Section The mixture interpretation tool also has a feature to removes alleles that are below the mPH setting If you click the box next to Remove Alleles Below mPH a check mark will appear in the box Click on it again to remove the check mark and dis
15. 12 13 45 67 8 9 10 5 8 9 1516 9 10 11 11 12 6789 7 910 XY 78 9 17 18 Ladder ide 8 9 10 24 24 2 6 7 8 9 6 7 8 9 12 13 4 5 6 7 89 10 5 8 9 15 16 9 1 1 11 12 67 89 7 930 XY 7 89 17 18 Ladder ide 8 9 10 16 7 8 9 6 7 8 9 1213 4 5 6 7 910 5 89 1516 9 10 11 11 12 167 89 7910 xv 7 8 9 17 18 Ladder 6 7 8 9 6 7 8 9 12 13 4 5 6 7 89 10 589 15 16 9 10 11 11 12 67 89 7 9 10 Xv 789 17 18 5614 12 13 28 29 30 9 10 110 11 12 1516 6 93 8 11 12 912 13 19 20 13 14 15 17 amp 11 14 151 Xv 912 22 22 2 5614 12 13 28 29 30 9 10 10 1112 1516 693 811 12 912 13 19 20 13 14 1517 811 141517 Xv 912 22 22 2 0156 34 12 13 28 29 30 9 10 10 12 12 1516 693 8 12 12 9 12 13 1920 1314 15 17 8 11 14 1517 xv 912 22 22 2 5634 12 13 28 29 30 9 10 10 11 12 15 16 6 93 812 12 932 23 1920 13 14 15 17 amp 11 151517 XY 912 22 22 2 59 14 12 13 28 29 30 9 10 110 1112 6 93 8 11 12 9 12 13 1920 1314 1517 amp 11 14 15 17 XY 9 12 22 22 2 X 01591 12 13 28 29 30 9 10 10 11 12 93 8 1112 9 12 13 1920 13 14 1517 8 1 14 15 11 XY 912 22 222 XX0161 16 13 14 11 12 1910 1418 66 89 11 13 1724 1314 1417 8 11 13212 xy 12 13 2226 Xx0161 14 13 14 11 12 1010 1
16. 1752 1881 853 967 10 ios 2em i iss 1091997 622560 717 679 751 637 57 708 827 795 1052 114 802 1489 1352 1456 637 727 748 2 7403 6117 2224 2344 2584 2804 4063 2468 1997 2155 1827 2560 2265 2316 2427 23842157 __2833 265 787 410 886 292 332 934 423 602 1632 1025 278 243 629 1264 992 669 438 1309 308 927 951 178 360 692 550 900 363 542 358 338 371 246 402 332 328 377 227 443 1 729 5216 3296 522 308 1613 2253 2379 1564 467 3127 428 2301 2167 377 1146 1409 1679 2011 415 1653 413 571 1531 373 1284 455 1483 1640 511 192 11463 1498 1057 983 1531 1281 1411 1183 1104 2333 1517 1622 12121001 1363106 110410277 1263120 Ed PEN a SUE aS Secr Sea scu icm a Ree eer re Se capt NN 3711 4982 925 2307 2315 505 4032 2418 542 2356 2776 1886 2548 2738 1519 395 1716 358 2295 1936 704 2445 2058 378 2051 370 1653 359 397 2085 675 8761 48864163 3028 3146 3462 3751 5470 7758 6758 2441 1948 3743 3440 4068 3903 3528 3185 4532 403 IPSE SS Se I EEG NSEINMSGSA CEN AI SM ELEC Eee ee 3915 4443 3448 3618 4644 2698 2497 2333 2426 2489 2309 2299 2202 2328 2316 2449 2410 27342721 2968 330 7403 6117 2224 2344 2584 2804 4063 2468 1997 2155 1827 25602265 23162427 23842157 2833265 5s06 4931 3394 2582 2439 3317 5361 5561
17. 83 ARMEDXPERT USER S MANUAL Likelihood Ratio XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 1 XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 Locus Locus Profile Allele 1 a Allele 2 b Allele 3 c Allele 4 d Likelihood 0 0308 0 3539 0 14799409 0 0139 0 3556 013653025 0 0542 0 2906 0 11888704 PR FGA 22 22 2 24 25 22 222 24 3 j a ee ee PO o 22 22 2 24 25 P1 o 22 22 2 24 25 0 1888 0 0128 0 1378 0 0689 600 0 16670889 0 225 0 0139 0 1861 0 275625 0 1773 0 0123 0 1256 0 1379 0 20529961 LU 1 Caucasian 3 9534E47 39 5 Million Black 1 8501E 9 1 85 Billion Hispanic 2 9496E 8 294 Million 1Px 0 2Px 1 Known Sources FBI i Mil ir dx Numerator Reset Knowns jo 2Px 1 set 9 Denominator Reset Unknowns Pick via mouse 28016 38 553333K1 11 Blood Vict 06 06 20 G02 FIGURE 7 41 Bottom of Likelihood Ratio Page To aid in the Likelihood Ratio calculations there are options at the bottom of the screen 1 Px is the number of unknown contributors for the prosecution and in this example is zero 2 Px is the number of unknown contributors for the defense and in this example is one Enter the correct numbers for each and click the Set button Note The top tow of alleles at each Locus is the Numerator or Prosecution and the bottom row of alleles at each Locus is the Denominator or Defense The calculati
18. ArmedXpert to open the main menu The left portion of the menu lists the following commands Import Export View and Print On the bottom right hand corner is the ArmedXpert Options and Exit ArmedXpert buttons Green ArmedXpert Icon i ArmedXpert Basic Commands ArmedXpert Options and Exit ArmedXpert Armedxpert Options Exit Armedxpert FIGURE 2 1 ArmedXpert Menu ARMEDXPERT USER S MANUAL ArmedXpert Options Click on the ArmedXpert Options button to open the options or defaults for the program There are six main sections General Data Interpretation Quality Control Reporting and Admin Console On Administrator copies only They are listed on the left side of the ArmedXpert Option Window The options for each section are shown on the right hand side of the window The section highlighted indicates which section s options ate being shown For example in Figure 2 2 below General 1s highlighted on the left hand side so the General view options are shown on the right hand side of the window Once the options are set they will change for all current or subsequently opened files in ArmedXpert You can go back into option window and the options can be changed as necessary Click Ok to save the options you have selected Click Cancel to close the Option window without changing the settings To obtain the software version information click on the About
19. Frequency Calculations FIGURE 7 34 Frequency Calculations The first type of frequency calculations is a Single Source calculation To obtain Single Source Frequency Calculations click on the Single Source button The Select Pick via mouse window will appear Select the single source sample you wish to perform the calculations on with your mouse or click on the Warrow and select from the drop down list ick via mouse s FIGURE 7 35 Select Pick Via Mouse Window The Frequency Single Source page will appear as in Figure 7 37 shown on the following page All Loci and their corresponding alleles are imported into the page On the bottom of the screen you can select the relationship types to include in your calculations including Unrelated Full Siblings Parents and Offsprings Half Sibs Uncles and Neph s and First Cousins Unrelated by default is checked The system will automatically calculate the frequency for each Locus and for each selected relationship type by ethnicity At the bottom of the page are the calculations by ethnicity for the entire profile The calculations are shown in both exponential and alphanumeric form Examples of the Frequency Single Source Page are shown in Figures 7 37 and 7 38 on the following page J Unrelated Full Siblings Parents amp Offspring Half Siblings Uncles Nephews First Cousins FIGURE 7 36 Relationship Type Selections 81 ARMEDXPERT USER S MANUAL Frequency Singl
20. Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt c s Einpeealerejeres ua es esm ias pien vmi ien e pzem xvj 17 18 19 20 8 9 10 24 24 2 67 8 67 8 9 12 13 45 8 9 10 5 8 9 15 1617 9 101112 1 1218 67 7 9 10 XY 17 18 19 20 55 19 24 24 2 6 7 8 6 7 8 9 12 13 4 amp 3 10 9 15 1617 9101 12 1 1213 6 7 79 10 XY 7 89 17 18 19 20 EMEN MEETING ee 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 142 18215 17 18 19 8 11 114 15 17 X Y 22 22 2 24 25 01 12 13 28 29 30 9 10 110 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 X Y 22 22 2 24 25 12 13 126 29 30 9 10 10 11 12 15 16 6 93 8 11 12 9 12 13 19 20 24 25 13 14 142 1832 15 17 18 19 8 11 14 15 7 X Y 9 12 22 222 24 25 isi 28 211 12 10 1 amp 18 6 amp 9 inis i527 nua 1417 12 XY izi 2229 eee RE er em mentem pen n Se ee em mt EMO MEN NEN NEN 12 13 28 29 30 9 10 10 11 12 15 16 6 93 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 X Y 9 12 22 22 2 24 25 12 29 31 912 910 14 793 1214 9 12 1720 1315 1416 8 11 1618 XY 1113 1923 PETERET ET
21. XX0164 99XX9999Q1 2 LFS Vict 06 06 20 D02 6 9 3 0 0 0 XX0165 QA004 QA 06 06 20 E02 0 0 0 XX0166 Reag Blank 06 06 20 F02 a aa ae EDEN SS Eee eee ee Ee eee XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 6 9 3 X 13 0 0 0 9 10 10 11 15 1693 11 12 9 12 1925 14 182 1518 11 1417 XY 9 12 22225 XX0169 QA026 QA 06 06 20 B03 2508 X Y 0 0 0 XX0170 Reag Blank 06 06 20 C03 XX0171 Con 06 06 20 D03 0 0 o XX0172 Neg Con 06 06 20 E03 FIGURE 6 6 Find Foreign to Reference Example 63 15 15 15 12 12 12 13 12 14 12 13 13 ARMEDXPERT USER S MANUAL Find Foreign to Reference is used to find loci that do not contain the reference All loci that do not contain the reference are highlighted red DEMO 1 2 Component 4 Vict Mix Stains Analyst txt c re 075820 CSF1PO D351358 THO1 D135317 D165539 251338 D195433 vwa TPOX 018551 Amel bss818 Fea Ladder Identifiler 06 06 20 A01 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 8 9 15 16 17 Y 7 8 9 0 0 2 Ladder Identifiler 06 06 20 A03 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 8 9 15 16 17 0 0 0 2 Ladder Identifiler 06 06 20 F03 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 8 9 15 16 17 0 0 0 2 XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 02 XX0156
22. lt FIGURE 1 7 Connection Properties Window 4 ARMEDXPERT USER S MANUAL Finally click the Ok button to connect to the server Registering ArmedXpert When you start ArmedXpert before you register the Registration Dialog window shown below in Figure 1 8 will appeat Click the Copy ID button and then paste the ID in an email ot text document and email it to support nichevision com You will be sent a Serial Number by email Copy the Serial Number then click on the Paste serial button You can also just directly enter the number into the boxes Click button and your file is registered If you would like to use the program before you get the Serial Number just click the Start Trial Version button for full access until your registration is complete Hegistration Dialog Thank you for using the trial version of this software After purchasing a license send the registration ID listed below to sales nichevision com to recieve your serial number Registration Info ID 2 TEXFE OFMFR RODBO BF8DF Serial K Phone 330 252 2712 Email support nichevision com httpFwww nichevision com Trial Running Days to end trial period Finished Run times left Finished FIGURE 1 8 Registration Dialog window ARMEDXPERT USER S MANUAL ArmedXpert Menu rmedXpert has a main command menu Click on the green icon on upper left hand corner of
23. 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 X Y 9 12 122 222 24 25 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 E01 6 9 3 19 20 24 25 8 11 XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 FO1 9 10 110 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 x Y 9 12 122 22 2 24 25 XX0161 1 QA003F1 06 06 20 H01 8 11 0 0 0 12 XX0162 1 Reag BlankF1 06 06 20 B02 ERES pause Pe ae ee ae a Sa a CS ee ae 0 0 0 1 XX0162 2 Reag BlankF2 06 06 20 CO2 i a ee ee ee eee ee ee XX0164 99XX9999Q1 2 LFS Vict 06 06 20 D02 9 10 10 11 12 15 16 6 9 3 B 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 XY 9 12 22 22 2 24 25 XX0165 QA004 QA 06 06 20 E02 x Y 0 0 13 XX0166 Reag Blank 06 06 20 02 XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 15 16 6 9 3 0 0 0 12 9 10 10 11 15 16 953 11 12 9 12 19 25 14 182 1518 11 14 17 XY 9 12 222325 XX0169 QA026 QA 06 06 20 B03 0 o 14 XX0170 Reag Blank 06 06 20 C03 S E XX0171 Pos Con 06 06 20 D03 0 0 0 13 XX0172 Neg Con 06 06 20 03 S 0 0 1 FIGURE 6 7 Find Where Reference Includes Foreign Example Sources The Sources section allows you to compare one table with another Select the table using t
24. 2 24 25 XX0165 QA004 QA 06 06 20 E02 14 14 7 93 12 14 9 12 1720 1315 1416 811 1618 XY 1 113 19 23 XX0166 Reag Blank 06 06 20 02 Ea meas a as 4 E t amid a a XX0167 99XX9999K1 11 Blood Vict 06 06 20 602 6 9 3 3 8 11 1415 XX 1212 2224 14 17 ha s ETE XX0168 99XX9999K2 Blood Susp 06 06 20 H02 m Euar em om 36m oss paal iaa xv sz 222 XX0169 QA026 QA 06 06 20 803 12 15 3030 11 12 11 11 14 16 93 1213 1513 18 22 1215 15 18 8 8 13 13 XYv i u 2920 XX0170 Reag Blank 06 06 20 CO3 pee XX0171 Pos Con 06 06 20 D03 13 13 30 30 10 111 11 11 11 12 8 8 15 19 uu 11 11 RFUs are off scale above 6000 FIGURE 5 6 Example of a RFUs Check screen 54 ARMEDXPERT USER S MANUAL CODIS QC There are six types of CODIS Quality Control checks They are CODIS Alleles Allele Count Check X OSD PHR and Check multi These quality control checks will evaluate the samples on the active sample table to allow you to know if they are ready to be accepted by CODIS QPAR CODIS Allele Check OSD PHR Check Alleles Count x multi CODIS QC FIGURE 5 7 CODIS QC Commands To perform the checks click on each type of CODIS quality control check you wish to perform After clicking each command the check will be performed and your page will display the results of the check All errors will be highlighted A key will be displayed on the page to explain what the
25. 3 4 or 5 ungreedy Special Characters Escape Character n New line Wr Carriage return x Tab Ww Vertical tab 4 i Form feed a Alarm 3b Backspace ie Escape Nii name Named Character String Replacement Backreferences n nth non passive group 52 xyz In abce xyz 1 xyz in abc xyz g Before matched string After matched string Last matched string 5 Entire matched string 4 Entire input string Literal FIGURE 2 17 List of Regular Expressions 18 Any character except new line Xn alb aorb LJ Group Passive Group abc Range a or b or c abc Not a or b or c a q Letter between a and q A Q Upper case letter between A and Q 0 7 Digit between O and 7 in nth group subpattern Note Ranges are inclusive Pattern Modifiers g Global match i Case Insensitive Multiple lines Treat string as single line x Allow comments and white space in pattern e Evaluate replacement u Ungreedy pattern gt A 5 Available free from AddedBytes com ARMEDXPERT USER S MANUAL The bottom section allows you to change the table to use when checking controls If you would like to change the default table click on the Select Table button Click on the appropriate table on the left and click on the gt button The table will move to the box on the right Click on the Load button to l
26. 9350 0 7603 0 7393 0 8511 0 7123 0 9806 0 9160 0 9858 0 9390 0 9722 0 9535 0 9924 0 9781 0 9168 0 9894 0 8335 StdError 0 0105 0 0851 0 0490 0 0083 0 0529 0 0441 0 0189 0 0631 0 0528 0 0403 0 0394 1 5Dilution 0 8171 AveragePHR 0 8031 0 8583 0 7260 0 8461 0 9295 0 9154 0 7885 0 6404 0 8671 0 8119 0 7817_ Std Deviation 0 1533 0 0805 0 2111 0 1174 0 0730 0 0558 0 0832 0 1699 0 0827 0 1478 0 1601 Min 0 6719 0 7668 0 5904 0 7211 0 8543 0 8519 0 6947 0 5204 0 8025 0 6647 0 6633 Max 0 9716 0 9185 0 9693 0 9539 1 0000 0 9561 0 8536 0 8348 0 9604 0 9604 0 9639 StdError 0 0885 0 0465 0 1219 0 0678 0 0421 0 0322 0 0480 0 0981 0 0478 0 0854 0 0924 FIGURE 8 21 Example of the left hand side of the PHR Report 103 ARMEDXPERT USER S MANUAL A graph appears to the right of the Loci that displays the average PHR for each Locus by Dilution table 1 2000 1 0000 0 8000 0 6000 0 4000 0 2000 0 0000 D8S1179 D21811 PHR Average 1 5 Dilution CSF1PO 0351358 0198433 D18S51 Amel D5S818 FGA PHR Average 1 0 dilution 0851179 0351358 0199433 PHR Average 25 Dilution TemplateReport eo ue o co O FIGURE 8 22 Example of the right hand side of the PHR Report 0219511 CSF1PO 03
27. 995678 K3 Susp 1 Ref 06 07 14 F07 Contributors 3 5 FIGURE 7 52 Example of a 3 Person Mixture with a Sample added automatically As with all of the pages the calculation for the each ethnicity for the entire profile is located at the bottom of the page The calculations are shown in both exponential and alphanumeric form 90 ARMEDXPERT USER S MANUAL Tab Six Reporting he Reporting tab is used to run the available reports It has six types of reports C D Form Total Data Sample Comparison CMF PHR Report and Template Report This chapter explains each report and sample views of each Q y oT LE C D Total Samples Templete Form Data Comparison Report Report Reports Views Data QC Checks Match amp Comparison Interpretation Reporting FIGURE 8 1 Reporting Tab C D Form The C D Form report is the CODIS Disposition Form for CODIS Administrator Click on C D Form and the window shown in Figure 8 2 will appear CODIS Disposition Form for CODIS Administrator cy 51 4 oDiDispositionFormorCODISAdminiswator Category _ vo PROFILE ter conis Reason Ee EVIDENCE PROFILE for the FORENSIC INDEX This profile is from crime scene evidence is attributable to a putative perpetrator and is not from an item owned used by the suspect 7 rese oft EVIDENCE PROFLE Tor the FORENSIC INDEK has beor dete Fre source ofthe EVIDENCE PROFILE Tor t
28. ArmedXpert button on the bottom right hand corner of the window The first main section General provides flexibility for how you can view your data The first option allows double homozygotes to be displayed The second option allows ArmedXpert to remember window sizes and locations when reopening When checked all windows that are reopened will open in the location and size that it was when closed previously Nera am General view options Data Double homozygotes Interpretation PAS J Remember window sizes and locations Quality Control Match amp Compare Reporting List match amp compare references at top Admin Console Do not sort automatically Sort by total matches exact included Sort by exact matches Sort by included matches About Ok Cancel FIGURE 2 2 General view options ARMEDXPERT USER S MANUAL The third option is how the Match amp Compare feature is viewed move the references used during the match and compare feature from the bottom of the page to the top of the page as shown in Figures 2 4 amp 2 5 below click on the List match amp compare references at top box The four choice listed on the bottom of the Match Compare section allow you to choose sorting options for your Match Compare data Click on the circle next to the option of your choice Match amp Compare List match amp compare references at top Do not sort a
29. BC 1 3 combination s 29 30 phr 0 87 p 0 70 Ref 1 28 p 0 30 For a 2 contributor 3 allele mixture of types AB amp BC 2 3 combination s 29 30 phr 0 60 p 0 52 Ref 1 28 29 phr 0 60 p 0 48 29 30 phr 0 48 p 0 56 Ref 1 28 30 phr 0 48 p 0 44 FIGURE d 8 Reference Selection Box with Reference Selected Settings The Peak Height Ratio PHr Minimum Peak Height mPH Minimum Proportion mP Homozygote threshold HT and Stutter are defaulted from the Mixture Interpretation Settings discussed in Chapter 2 You can change these values on the arrows next to each box to appropriate value in accordance with your individual lab validated thresholds The changed value will remain active as long as the Mixture Interpretation Window stays open or you manually change it to another value The default values set in your Mixture Interpretation Settings will appear when a new Mixture Interpretation Window is opened The Lock locus option allows you to lock in the settings for the current locus allowing settings to be changed without altering the final report Mixture Interpretation Quality Control Frequencies Frequency H Mixture interpretation settings Allel m Default peak height ratio 0 3 V BelowmeH PH 175 HT 100 5 mP 0 00 3 4 Default minimum peak height mPH 175 Lock locus dM a s Default homozygote threshold HT 100 Default minimum proporition mP 0
30. Chapter 7 Tab Five Interpretation 65 Mixture nterpretatiOfi outset arie tin esatta E eee potu uve utabus Ec tUe usen tuae Un no 65 Mt OPI pt o1 Chapter s Tab Six RODOELUTHD os oco oeconomiae 91 MM E 91 TO BC t 94 Samples COMPA SOD reniris renen na eE A EEUE IREE AE EE ONORE 95 One A M f PHRRE DON mE cm 99 sailor 104 f 105 ARMEDXPERT USER S MANUAL Introduction ArmedX pert Developed by analysts for analysts rmedXpert allows the user to check ladders check controls check for possible stutter perform matching between evidence samples and references check for potential contamination with staff profiles do Mixture Interpretation with two to three biostatistical analyses for single and multiple soutce samples print and save data ArmedXpert consists of six tabs and an ArmedXpert menu The ArmedXpert Menu is used to set default options and perform basic functions such as importing exporting and printing The tabs are used to navigate through the different functions of ArmedXpert Chapters 2 through 8 go through each of these in detail Q z ArmedXpert aJ Data QC Checks Views Match amp Comparison Interpretation Reporting FIGURE 1 1 ArmedXpert Tabs Import Export View b Print d SETI Armedxp
31. Data Frequency analysis or Mixture interpretation if you wish to save it and resume it later You also have an option to save any page in a workbook friendly format so that you can open it in Excel or as an Image When you click on Export the options below will appear They will be shown in color once you have selected a table on the screen However if you do not they will show as gray as seen in Figure 2 30 To save a page data click on Page Data A Save As Window will appear You can name the page anything you wish The default folder to save a page data is in the Page Data folder located in ArmedXpert Folder However you can change the folder to save it to Once you have chosen the name and folder click the Save button Page Data Save in the page data Format Excel Workbook Save as a workbook or in a workbook Friendly Format Image Save the entire worksheet or selected View gt range as an image Frequency Analysis Save a frequency analysis page so that it Print an be resumed later Mixture Interpretation Save mixture interpretation so that it can be resumed later Armedxpert Options Exit Armedxpert FIGURE 2 30 Export Menu 28 ARMEDXPERT USER S MANUAL Save in 6 Page Data Iv amp 5 BHEGHI Mixture Data xls mix file complete Tb a xls My Recent mix file complete Tb analyzed xls Documents B mix file complete Tb xls B mix file complete TB
32. Datasource Commit Table window with filter being used The table name and Type kit will be filled in you can change these if wanted Once finished choosing samples and all info is filled in correctly click the Commit button When finished with the window click the Done button to close the window Comma tem s Table Individual Proliles i gt Table Name Ladder and Controls Type kit Iderhfie Comme Done FIGURE 4 13 Datasource Commit Table bottom portion of window 47 ARMEDXPERT USER S MANUAL You can commit individual profiles such as staff profiles as shown in Figure 4 14 below Click on the appropriate sample table so it is open and active on the screen Then click the Commit Sample to Data Source button All the individual samples will show on the right side of the window our staff profile example is shown as alphanumeric as opposed to names Click on the sample you would like to commit and click the gt button to move it to the left side of the window or click the gt gt button and all the samples will move to the left side of the window Q Views Data QCChecks Match amp cComparison Interpretation Reporting New Edit Change Combine Commit Samples Load Samples Connect Disconnect Sort Sample Sample Kit Sources to Data Source From Data Source Server Server Samples Data Source Sort C Program FilesWlicheVision Inc Arme dX pert data GMID_Tables Identif
33. QA 06 06 20 E02 XX0166 Reag Blank 06 06 20 F02 XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 XX0168 99XX9999K2 Blood Susp 06 06 20 H02 XX0169 QA026 QA 06 06 20 B03 XX0170 Reag Blank 06 06 20 C03 XX0171 Pos Con 06 06 20 D03 XX0172 Neg Con 06 06 20 E03 XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 H02 14 E FIGURE 8 10 Samples Comparison Report example 96 ARMEDXPERT USER S MANUAL CMF The CMF form is the CODIS upload file When you are ready to upload your data to CODIS click on CMF and the CMF window will open as seen in Figure 8 11 below Click the Next to the Sample Table to expand to a detailed list of all samples Choose the Samples you would like to upload by clicking on them and clicking the gt button The samples will move to the box on the right as seen in Figure 8 11 below You can also use the Selection Filter box to enter in a specific name and all samples containing that name will be highlighted This is helpful in finding certain samples in a large sample table Click the gt button and all samples highlighted will move to the right hand box o C M F m Samples amp sources Selected samples amp sources DEMO 2 Component 4 Vict Mix Stains amp nalysttxt A Ladder Identifiler 06 06 20 F03 Ladder Identifiler 06 06 20 403 Ladder Identifiler 06 06 20 401 1 99999901 15 2F1 Shirt Vict 06 06 20 2 lt 0156 3 994 lt 999901 15 2F3 Shirt Vict 0
34. THO1 6 9 3 6 93 i Add Combo v 49 10 49 Any 310 10 410 Any 4 2p for 3 4 2p for 10 Unrestricted RMP Modified RMP lv Pick via mouse Contributors 2 FIGURE 7 47 Adding a combination to the Mixture Frequency Page ARMEDXPERT USER S MANUAL To remove a combination place your mouse over the combination The combination will appear gray and will have a red line through it as shown below in Figure 7 48 Double click on the box and it will be removed It can be added back at any time kd FIGURE 7 48 Removal of Combination When either one of the RMP combos or the 2p combos are chosen the math used will appear in blue next to the combo when you have your mouse over the combo as shown below in Figure 7 49 Click on any of the left of the ethnicity and the math for that type will be shown Add Combo v 28 29 30 Add Combo V 28 29 307 E Cau casian 0 1658 0 1811 0 2321 EH Black Hispanic Add Combo M FIGURE 7 49 Math shown You can also see the math calculations for all combinations by clicking on the calculations tab on the bottom of the page Mixture Frequency XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 c s X XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 D851179 ip q 12 13 0 1454 0 3393 0 1083 40 2222 0 1207 4
35. User View Ft Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt LII 262 27 262 27 123 127 185 187 255 305 309 112 6 166 217 221 252 264 309 312 316 102 106 109 154 159 163 222 262 27 1 200 268272 322 320 124 171 186 229 233 268 281 325348 121139 17 183 242 291 30 FI40 144 200 204 208 267 271 322 326 330 124 128 171 186 217 229 233 268 280 285 324 329 344 348 117 121 123 139 171 179 183 187 230 242 291 295 140 144 200 204 208 267 271 322 326 330 124 128 171 186 217 229 233 268 280 284 325 329 344 348 117 121 123 139 171 179 183 187 230 242 291 295 1 140 144 200 204 208 267 271 322 326 330 124 128 171 186 217 229 233 268 280 284 325 329 344 348 117 121 123 139 171 179 183 187 230 242291 295 244 149 204 210 272 276 3 22 i24ize 171 229 233 276280 312 325 uin 16519 moce 29 SER NEP LONIBIERONCRNE NY ERN T40 144 200 204 208 267 271 322 326 330 124 128 171 186 217 229 2331208 280 284 324 329 344 348 117 121 123 139 171 179 183 187 230 242 291 295 149 200 212 265 271 318 322 120 175 176 233237 268 276 316 324 113 117 185187 230242 299 30 140 144 204 208 272 322 330 124
36. by allele count FIGURE 2 7 Mixture Interpretation settings This section also has two save options available for the Mixture Interpretation Window The Prompt to save on window close option will bring up a confirmation window asking you to save or not when you close the Mixture Interpretation Window The Prompt to save on application close option will do the same thing if you close ArmedXpert and the Mixture Interpretation Window 15 open Confirmation wali J Save interpretation of lt 72 2 9925679 Q1 2 F2 Vag Swab 06 07 14 D02 Yes No Cancel FIGURE 2 8 Confitmation Window 10 ARMEDXPERT USER S MANUAL The bottom section is the Profile naming scheme section It allows you to set up names for the profiles in the Mixture Interpretation Window The left hand side of the section is to set up default names Add a check mark next to each item that you would like to have appear in the name The Preview section will show you an example of what your profile name will look like The text can be changed to any text that you wish Profile is shown as an example Auto number will add another number to last number used during the time the software is opened So the numbers will start at 01 and proceed up from there The Date and Time field add the current date or time to the profile name at the time the mixture interpretation window was opened The Sample Name will add the sample name used for the mixture interpr
37. highlighted colors mean for the check being done If no errors are found on the check a message will appear at the bottom of the page that states Check completed with no errors as shown below in example 5 8 Click on the Clear Checks button after each check to clear the check results on the page z ArmedXpert Views Data QC Checks Match amp Comparison Interpretation Reporting amp WOPR a Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begi Controls Ordering Alleles Count X multi Revie Standard Checks CODIS QC QCRe C Program Files NicheVision Inc ArmedXpert data Saved_Profiles Page_Data MT Test Genotypes Table txt Pp 82179 1021911 D7S820 CSFIPO D351358 D135317 D165539 D251338 D195433 vwa TPOXIDISSS 01 Identifiler Kit Standard 10 10 04 01_ Mixture 0 Neat 10 10 04 Mix 10 11 14 29 30 8 9 10 12 910 11 1617 8 9 93 11 12 1 12 17 18 20 26 12142 151617 18 19 8 8 1 18 XY Mixture M F Neat O 10 10 04 Mix 11 14 2929 9 10 910 16 16 89 1 11 1 12 1718 14215 18 19 188114 xy i Check completed with no errors Mixture M 1 1 10 10 04 Mix Mixture M F 1 10 10 10 04 2 Mix Mixture M F 1 100 10 10 04 2 Mix Mixture M F 1 4 10 10 04 Mix Mixture M F 10 1 10 10 04 2 Mix Mixture M F 100 1 10 10 04 2 Mix Mixture M F 4 1 10 10 04 Mix FI GURE 5 3 Example of a Check completed with no errors s
38. resumed later Pick via mouse Ml 172 2 3395678 Q1 2 F2 Vag Swab 06 07 14 D02 Locus D8S1173 6 e Protiie 41 918 P Avg 0 39 PH 0 60 gt E Profile 42 12 14 P Avg 0 28 jgjlmeeAeles ur agg t Alleles 9 11 12 13 14 18 Profle 43 11 13 P Avg Ud4 mM mtr U RFUs 2344 2078 1439 1756 1151 2030 I Loeklocus BPs 127 135 140 144 149 157 ai T Popout View call report cals Add Comment 3 11 12 13 14 18 0 08 Mixture Information For a 3 contributor 6 allele mixture of types AF amp BD amp CE 1 15 combination s 9 16 phr 0 87 p 0 41 Profile 41 Ref 1 12 14 phr 0 80 p 0 24 Profile 42 Ref 2 11 1 FIGURE 2 33 Mixture Interpretation page with Export Menu 30 ARMEDXPERT USER S MANUAL View Click on View to view your stored profiles To initially add the profiles see the instructions under the Data Source section in chapter 4 Click on Profiles and a Chose value window will open Select the type of profile you would like to view Click the Ok button The profile type table will open Export Armedipert Options Exit Armedxpert FIGURE 2 34 View Menu Choose value cs Staff m Ok Cancel FIGURE 2 35 Chose value Window 31 ARMEDXPERT USER S MANUAL Print To print a page click on the print option The print window wil
39. subsection Options contains the settings that are used during certain RFU and CODIS Quality Control Checks The top section allows you to set the RFU quality control options You can set the Low scale data figure and the Off scale data settings by entering the required amount in each box The bottom section allows you to set the CODIS quality control options You can set the OSD off scale data and the PHR Imbalance settings by entering the required amounts in each box ArmedXpert Options n x General Options Ladders and Controls Data RFU quality control options Interpretation Low scale data Juality Contro Reporting Off scale data CODIS quality control options Admin Console OSD off scale data PHA Imbalance Ok Cancel FIGURE 2 15 Quality Control Options window 16 ARMEDXPERT USER S MANUAL The second subsection Ladder and Controls contains the settings that are used during the Check Controls Quality Control Check The top portion has the Control qualifiers These can be changed by clicking on the Expression column in the row that you would like to change and then change to the correct expression The expressions are using regular expressions This allows for all controls to be picked up during the quality control check For example Ladder will pick up any control that contains the word Ladder no matter what characters are before or after the word ArmedXpert Op
40. that name will be highlighted This is helpful in finding certain samples in a large sample table Click the gt button and all samples highlighted will move to the right hand box As shown below in Figure 4 12 the word Vict was added to the Selection Filter box and all samples that include the word Vict are highlighted The Ignore Case option allows all forms of the filter to be highlighted regardless of lower or upper case The Also select tables option allows for tables to also be highlighted In the example shown below the Also select table option is not checked so even though the table contains the word Vict it is not highlighted 1 Datasource Commit Select items to commit to the datasource a DEMO_I_2 Component_4 Vict Mix_Stains_Analyst txt Ladder Identifiler 06 06 20 F03 Ladder Identifiler 06 06 20 403 Ladder Identifiler 06 06 20 401 220156 1 999990 1 15 2F1 Shirt Vict 06 06 20 L 220156 3 99699990 1 15 2F3 Shirt Vict 06 06 20 E 220159 1 99699990 1 15 8F1 Shirt Vict 06 06 20 F 220161 1 GA00SF1 06 06 20 H01 0162 1 Reag BlankF1 06 06 20 B02 0162 2 Reag BlankF2 06 06 20 C02 2080164 996999901 2 LFS Vict 06 06 20 D02 20165 QA004 QA 06 06 20 E02 gt x 0166 Reag Blank 06 06 20 F02 ond A157 33 3333K1 11 Blood Vict 06 06 20 402 y E wv IVA Ia 8 AV ie ee oe 00 j lt Vict Ignore Case Also select tables It items as FIGURE 4 12
41. the Mixture Interpretation Window It will be added in a column next to the evidence information as shown in Figure 8 5 ARMEDXPERT USER S MANUAL 9 8973999 RAS P Reo he Correct results were obtained for all controls Evidence XX72 2 99XX5678 Q1 2 F2 Vag Swab 06 07 14 D02 2344 2078 1439 1756 1151 2030 30 30 2 8 9 10 12 CSF1PO D351358 6 7 8 9 9 3 9 10 11 12 1218 1736 2292 1034 0251338 20 25 D195433 14 14 2 15 15 2 3226 1520 751 937 W 14 16 17 18 2365 1805 1211 912 TPOX D18551 13 Amel KT 4261 1602 X Y Sl O Evidence Victim _ Define Alleles for O Suspect Elim Evidence zii D135317 P lt gJ oloo t J M Co o un eju un co in un Nie e UJ wW wo Is FIGURE 8 5 Define Alleles for Evidence On the bottom of the form is a place for the analyst to initial and date as well as a place for the tech reviewer to initial and date coos D jon Form for CODIS Administrator fee uo 5 esu l Cee NO PROFILE for CODIS Reason EVIDENCE PROFILE for the FORENSIC INDEX This profile is from crime scene evidence is attributable to a putative perpetrator and is mot from aa item g 0O 5 Fhe source of the EVIDENCE PROFRE for the FORENSIC INDEX has been identified L The source of the EVIDENCE PROFILE for the FORENSIC INDEX is unknown
42. threshold is met Locus by Locus If the threshold is not met it will indicate that there is potential stutter The stutters settings are set up under the quality control subsection of the Interpretation section in the ArmedXpert Options Menu All alleles that should be evaluated for stutter are highlighted in pink Q ArmedXpert C Program Files NicheVision Inc ArmedXpert data Saved_Profiles Page_Data l0A 2904 xls Views Data QC Checks Match amp Comparison Interpretation Reporting 8 MNACPJSES a Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Clear Controls Ordering Alleles Count x multi vedi Checks Standard Checks CODIS QC QCReview Clear eus T orso esr messe os v ror os na FA 052504 Known standard Tama aa Tra fon ee hel aaf re fes es paralea wn etn aa 92904 Known standard of victim LE r en ee eu hu ee mal sa enfeeble aa 092904 stutter fiter 930 3 sna 17 WISIS 5 1 12 13 a EEIE 1QS2904_Stutter fiterappled 15 4 253536 73102 27 15 37 9 9 an 12 33 B 1819 13 14 15 6 amp 17 8 XX 11 3 2 2 24 FIGURE 5 5 Example of a Check Control screen Finally RFUs indicates all RFU s that are either below scale or off scale The RFU s settings are set up under the Quality Control Section in ArmedXpert Options Menu The alleles with RFUs below scale are highlighted pink and all that are off scale are highligh
43. txt Zun Usermame admin LS FIGURE 2 37 Print Options ARMEDXPERT USER S MANUAL Tab One Views he first tab in ArmedXpert is Views There are five main sections of the View tab View Fit Zoom Show Hide and Windows This tab s commands allow you to choose how the data appears on your screen views Data Oc Checks Match amp Comparison Interpretation Reporting Alleles Samples R Headings Controls Base Pairs All 3 Indicate clipped data Zoom 100 Organize J Samples RFUs User View Fit Zoom Show Hide Windows FIGURE 3 1 Views Tab View The View section gives you the option of what type of data is displayed The left side of the view section allows you to choose between viewing Controls Samples or All Controls and Samples The right side allows you to choose whether Alleles Base Pairs or RFU s are shown Click on the circle to the left of the desired choice Examples of all different view combinations are shown below ai All Alleles Controls C Base Pairs Samples C RFUs view FIGURE 3 2 view Section 33 ARMEDXPERT USER S MANUAL Qo J ArmedXpert Views Data QC Checks Match amp Comparison Interpretation Reporting Alleles Samples R E Headings m Controls C Base Pairs C All A Indicate clipped data 2 Zoom 100 Organize _ Samples _ RFUs User L View Ft Zoom
44. were combined into a new sample table XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 XX0159 1 99XX999901 15 8F1 Shirt Vict 06 06 20 F01 XX0156 3 99XX999901 15 2F3 Shirt Vict 06 06 20 E01 15h10m48s D851179 Dnsh osse csripo 0351358 m A ees 7 D165539 D251338 D195433 vwa TPOX D18S51 Amel D5S818 FGA 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 142 18215 17 18 19 8 11 14 15 17 X Y 9 12 22 22 2 24 2 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 X Y 9 12 22 22 2 24 2 12 13 28 29 30 9 10 10 11 12 15 16 17 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 X Y 9 12 22 22 2 24 2 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 E01 XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 F01 FIGURE 4 9 Combined Sample table Example 45 ARMEDXPERT USER S MANUAL Data Source The four commands in the Data Source Section are Commit Samples to Data Source Load Samples From Data Source Connect Server and Disconnect Server The Commit Samples to Data Source allows you to save ot commit a sample to your database and the Load Samples to Data Source allows you to retrieve it Examples of uses for this are to commit your sta
45. 0 3251 D21511 p q r 28 29 30 0 1658 0 1811 0 2321 0 2151 0 1899 0 1788 0 069 0 2044 0 3301 D75820 2pq p p 1 p Theta 2 9310 10 10 1 10 Theta 0 08590136 0 0865098764 0 10173796 0 1070359756 0 02933396 0 0958828556 CSF1PO p p 1 p Theta 2p 1 p p p 1 p Theta oss w Pick via mouse Contributors 2 FIGURE 7 50 Calculations Page 89 ARMEDXPERT USER S MANUAL You can automatically add a sample to the Mixture Page Go the bottom of the window either click on the Pick via mouse button and select your reference or click on the Warrow and select the sample from the drop down list of samples This will automatically fill in the allele combinations for that sample and all calculations will be done automatically This section is also where you can indicate whether this is a two or three person mixture Use the up and down arrow to alternate from 2 to 3 v a Pick via mouse Contributors 2 FIGURE 7 51 Bottom Portion of Mixture Window Mixture Frequency XX72 2 99XX5678 Q1 2 F2 Vag Swab 06 07 14 D02 c f S Locus d a e Amel dE dE l Add Combo Y FGA Add Combo lin 1 0912E 18 1 09 Quintillion lin 6 918E 20 691 Quintillion lin 1 4551E 18 1 45 Quintillion Theta 0 01 Contributors 3 M 4 MJ Frequencies Calculations lt ji i Pick via mouse 95
46. 0273 sorv or 0 0316 0 0344 0 0405 wpiv o orv or 0 0149 siv o 0 0308 0 0568 0 0352 0 0087 0 0061 BRERR 2285 ESTR PHR Average 1 0 dilution 158110 sco osaa osan 0333 osm osz2 oss 0 9928 0 7603 0 7393 09378 07186 1 82 Long 4802 0 9806 _0 8708 ossss osso osas osss ossso osseo 0 9168 0 9894 0 8335 10 83 10ng 1 A03 0 9607 osio os osu oz osws 0 9363 0 7893 ossu 07123 4 Std Deviation 0 0183 01474 oiv o 0 0849 0 0143 0 0915 oiv o sprv o 0 0764 0 0 0328 0 1093 0 0915 0 0699 0 0682 in 0 9441 0 6411 0 0000 0 8333 0 9112 0 7898 0 0000 0 0000 0 0000 0 8045 0 0000 0 9350 0 7603 0 7393 0 8511 07123 i SG DEORE i Max 0 9806 0 9160 0 0000 0 9858 0 9390 0 9722 0 0000 0 0000 0 0000 0 9535 0 0000 99024 0 9781 0 9168 0 9894 0 8335 RES stdtmor 0 0105 0 0851 sprv or 0 0490 0 0083 0 0529 spiv oi prv or 0 0aa1 0 0 0189 0 0631 0 0528 i 25 Dilution 551 0253 9toi 0 9716 0 8896 ose osa ose 0 8348 08925 see 3 025ng 7003 0 6719 0 9185 0 5904 osss ossa oss 0 8536 _0 5661 osssa ossoa 0 9630 12909 10 02 025g 8 002 0 7659 _0 7668 0 9693 0 7211 1 00001 ossm 0 6947 0 5204 9604 oso7 o 7181
47. 0906 0 0334 0 0679 0 1269 0 0788 0 0194 0 0136 Min 08652 0 8369 0 8438 0 7841 0 7827 0 9067 0 8093 0 6037 0 7957 0 9246 0 9232 eo e Max 09716 0 9629 0 9861 0 9570 0 9667 0 9840 0 9799 0 8561 0 9862 0 9710 0 9592 RESP std Error 0 0254 0 0273 0 0316 0 0344 0 0405 0 0149 0 030 0 0568 0 0352 0 0087 0 0061 BR 8 PHR Average 1 0 dilution 10 82 10ng 4 802 09806 0 8708 0 9858 osseo 08943 0 9535 _0 9350 osseo _0 9168 0 9894 0 8335 10 83 10ng 1 A03 0 9607 0 9160 0 8447 _0 9112 07898 0 8045 0 9363 _0 9781 0 7893 0 8511 07123 Average PHR 0 9618 0 8093 0 8879 0 9231 09855 0 8602 0 9546 0 8748 0 8151 0 9261 07548 0 1474 0 0849 0 0143 0 0915 0 0764 0 0328 01093 0 0915 0 0699 0 0682 mm 0 9441 0 6411 0 8333 0 9112 0 7898 0 8045 0 9350 0 7603 0 7393 0 8511 07123 Max 0 9806 0 9160 0 9858 0 9390 0 9722 0 9535 0 9924 0 9781 0 9168 0 9898 08335 2 5 Std Error 0 0105 0 0851 0 0490 0 0083 0 0529 90441 0 0189 0 0631 0 0528 0 0403 0 0394 BRR RTE w ID D 3 0 25ng 7 00 0 9639 AveragePHR 0 8031 0 8583 0 7260 0 8461 0 9295 0 9154 0 7885 0 6404 0 8671 0 8119 0 7817 Std Deviation 0 1533 0 0805 0 2111 0 1174 0 0730 0 0558 0 0832 0 1699 0 0827 0 1
48. 1 2324 XX0172 Neg Con 06 06 20 E03 ppp eee eem pee ree eene een ee Se FIGURE 2 27 File opened in ArmedXpert 26 ARMEDXPERT USER S MANUAL Click on Frequency analysis to import in a previously exported Frequency analysis calculation page All exported Frequency analysis will have an axf extension The Frequency analysis calculation page will import in with all the information that was exported out You can now continue using the Frequency analysis calculations Click on Mixture Interpretation to import in a previously exported Mixture Interpretation All exported Mixture Interpretations will have an axm extension The Mixture Interpretation window will open along with a copy of the sample table which contains the sample that the mixture interpretation window was using All calls made before the Mixture Interpretation was exported will appear along with the settings used and references applied You can now continue using the Mixture Interpretation tool ArmedX port Q Data QCQOeds Match Companson Interpretebon Reporting a gt 14182 5 15 16 6 931 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 XY 9 12 22 22 2 24 25 zr EE 9 12 22 222 24 25 15 17 X Y 9 12 22 _12 Xxv 1213 2 26 meg 515 17 XY 9 12 22 22 2 24 25 18 xv 1113 19 23 Ez remm XWDI56 9330 99999 105 2F She Viet 0606 2000 Contatti
49. 121510 xls 5 5 P File name My Network Save as type 5 xls FIGURE 2 31 save As Window To save a Frequency Analysis so that it can be resumed later in ArmedXpert click on Frequency Analysis The Save As Window will appear You can change file name to whatever name you would like and Save it in whichever folder you would like Click Save once you have the file name and folder chosen You also have an option to save a frequency analysis as an excel workbook by clicking on Excel Workbook when you have the Frequency page open You can then open the Frequency analysis in Excel Finally you can save the report as an image by clicking on Image Page Data EP Import Save in the page data Format Excel Workbook Save as a workbook or in a workbook friendly Format Image Ex Save the entire worksheet or selected View gt range as an image Print gt Mixture Interpretation Save mixture interpretation so that it can amp be resumed later Armedxpert Options Exit Armedxpert ArmedXpert Reporting urce XX94 99XX5678 K1 5 Vict Ref 06 07 14 E07 c3 5 E X5678 1 5 Vict Ref 06 07 14 E07 13 Frequency 2 Frequency Frequency ae Parents an 2 Unrelated Full Siblings 1 Allele 1 Allele 2 Offspring 2 ha 9 16 L 1 2 0 0128 0 0128 0 00032768 0 25
50. 128 171 186 217 23 280 285 329 344 115 13 179187 2022 29129 144 200 259272 322 326 124 171 186 229 233 26528 325348 121 139 171 183 242 29130 140 144 208 275 279 322 132132 179186 229 237 230237 316 328 117 121 179 187 234 29 me Des cdam as OE spon POLEN por Eom er ee 2 ZL275 325330 uo imus 186 229 276280 32430 121125 179183 230 353 pem Se ee ce nc PRC FIGURE 3 6 View All Samples amp Controls by Base Pair Qo 9 ArmedXpert Views Data QC Checks Match amp Comparison Interpretation Reporting Alleles Samples Headings oa zl ote kee 5 ei Controls 5 Base Pairs All Indicate clipped data Zoom 100 Organize _ Samples C RFUs _ User View Fit Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt D TPOX E 18551 mel 102 106 155 222 262 107 134 215 102 2 Bl 307 312 102 106 154 222 262 107 134 215 154 222 262 107 134 215 167 230 291 107 1 156 1235 N 52 264 N 76 280 312 325 117 121 268 276 316 324 113 117 316 328 179 18 w 280 23 eee 121 125 179 NNEZR N 76 28 FIGURE 3 7 View All Controls by Base Pair D ArmedXpert Views Data QC Checks Match amp Comparison Interpretat
51. 135433 4 Jl Alleles 13 14 14 2 18 2 DLrili 707 Ai oOoC 70 FIGURE 7 22 Highest to Lowest Feature To assist with your interpretation you can choose to visualize the proportions of your calls in a graphical format by clicking on the View call report button A report will open that will show each Locus for each profile and as you make your calls the alleles will auto populate the report A graph showing the proportion of each profile by locus is generated as shown in Figure 7 24 shown below You can customize how the report appears by clicking the arrow next to View call report button It will bring up a drop down list as shown below in Figure 7 22 NOTE The report only shows the Loci that you have completed using the Mixture Interpretation Window The graph only shows the proportions for the loci that have completed combinations called Helerences PHr 0 3 e mPH 175 mP 0 00 327 12 View call report 5 Chart ms Sort loci to show degredation 20 FIGURE 7 23 view call report button V I column Clustered 4s Column Stacked Column Stacked 100 29 Calls 28 iv Sort loci in default order 32 Sort loci by allele count 16 Sort loci to show degredation 76 ARMEDXPERT USER S MANUAL ArmedXpert Interpretation DEMO 2 Component 4 Vict Mix Stains Analyst txt 1ps8s1179 p21511 p75820 csF1PO D351358 THO1 D135317 D165539 D251338 D19S433 vwa
52. 2 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 01 aimee ate End tte tet E D dnd E d dnd d m dd oco e ooo ao as on en ol aan in su aa as as EEDECIOESEDEC EOROETEETEECEOEDETIEERECECEU ERE sas 1 QQ 9999N1 15 RF1 Shirt Viet n65 n6 20 Fn1 12 13 JR 29 an ain 10 11 12 15 16 amp Qala 11 12 q 12 13 13 20 24 25 13 14 142 1R2 18 17 19 A 11 17 x Y 9 17 122 222 24 FIGURE 3 19 Clipped Data Shown 39 ARMEDXPERT Organize The Organize section allows you to move a window to the top of all other windows This is a useful tool if you have several windows open at the same time Click on the Organize button and the window shown in Figure 3 21 will appear All opened windows including tables mixture interpretation windows frequency calculations and reports will appear Choose from the list the one you want to bring to the front and click on the Bring to Front button Your selected window will now be brought to the front of all other windows USER S MANUAL Click on the red X on the upper right hand corner of the Organize window to close it Organize Windows FIGURE 3 20 Organize Window Windows F jalx 5 Remove Group CULA DEMO I 3 Cemponent No Controls V 51 52 E tst DEMO_ _2 Component_4_Vict_Mix_Stains_Analyst txt B AM m Mixture Interpretation DDA Interpretation omg To ee Front
53. 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 XY 9 12 22 22 2 24 25 XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 F01 12 13 28 29 30 9 10 10 11 12 15 16 6 9 38 11 12 9 12 13 19 20 24 25 13 14 14 2 182 15 17 18 19 8 11 14 15 17 XY 9 12 22 22 2 24 25 XX016L1QA003F1 060620 80i iyu 76311 1 1010 1418 66 89 1 13 i252 1514 1 amp 9 fen 1212 xyz 2226 XX0162 1 Reag BlankF1 0606 20 902 ee 622 Reag BlankF2 0606 20 02 a a a a ee a a a IR SEES a a a XX0164 99XX9999Q1 2 LFS Viet 06062000 121 28 29 30 5 10 10 13 12 15 16 6 93 8 41 12 9 12 13 19 20 24 25 13 14 142 192115 17 18 19 11 14 1517 XY 9 12 22 222 28 25 90165 QA004 QA 0606 20 02 D166 seg ric 06 06 20 02 puce eee ee M NEN CES MEN EIE XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 12 13 29 30 10 10 10 12 15 16 6 93 812 1213 2024 13142 1719 8 amp 11 1415 XX 15 12 2224 XX0168 99XX9999K2 Blood Susp 06 06 20 H02 13 13 2828 9 10 10 11 15 15 693 1 12 9 12 1925 14182 1518 11 11 1417 XY 912 22225 XX0169 QA026 QA 06 06 20 803 12 15 3030 11 12 1 11 1416 193 12 13 1213 1822 1215 1518 88 1513 XY 1L 11 20 20 XX0170 Reag Blank 06 06 20 C03 ppp eec XX0171 Pos Con 06 06 20 D03 13 13 3030 10 111 10 12 1415 8 93 1 11 1 12 1923 1415 1718 88 1519 1L1
54. 2776 2916 21622181 53732181 3694 3181 32604 3926 35231 TEE ee ee mM EU 3811 aso 1814 17971 23162138 1473 1662 1319 1318 35786 19232310 13061299 15771483 1652 1621 mna adl NEN Birra acd E CN NM MCN Pct RC FIGURE 2 0 view Samples amp Controls by RFUs Q ArmedXpert l views Data QCChecks Match amp Comparison Interpretation Reporting C All Alleles Samples Headings x i ae lie ei Controls Base Pairs Indicate clipped data E Zoom 10095 Organize L Samples RFUs _ User View Rie Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt A Lim amo ns 1274 ee 0 03 1806 1454 1083 976 1428 1887 853 976 3416 1989 811 610 1565 2601 1315 560 717 791 637 706 827 1032 1489 637 730 2491 1549 608 449 1463 1057 1531 1411 1183 2333 1517 1212 1363 1104 1263 1137 1186 1066 1182 816 1 RR pepe P vau a mnm a 8761 4886 3028 3462 5470 17758 2441 LIII Sa a 5396 3394 2582 3317 5361 2776 sme opus 3811 4250 1814 2316 1473 1319 3578 1923 1306 1577 1652 2685 ee e FIGURE 3 10 view All Controls by RFUs Q p ArmedXpert views Data QCChecks Match Compariso
55. 3 233 9 237 8 241 8 245 73 249 75 D18S51 7 9 10 10 2 11 12 13 13 2 14 14 2 15 16 17 18 2491 1483 1948 1006 1680 2404 1651 778 1128 261 9 270 06 274 16 276 18 278 27 282 4 286 47 19 20 21 22 23 24 25 26 27 731 902 1039 1051 835 762 661 774 1011 1038 288 5 290 62 292 66 294 79 298 97 303 35 307 77 871 934 1091 1147 312 22 316 53 320 8 325 1 329 19 333 33 337 42 341 34 344 99 D5S818 7 8 9 10 11 12 13 14 15 16 608 658 573 576 568 488 524 433 440 467 133 99 138 11 142 53 147 02 151 4 155 65 159 77 16333 16799 1724 FIGURE 8 7 Total Data Report 94 ARMEDXPERT USER S MANUAL Samples Comparison The Samples Comparison compares one table against another This report is useful in comparing your staff profile table to a sample table to confirm that there was no contamination This is the same report that runs automatically if you have Compare tables to staff profiles upon opening checked in the Reporting section of the ArmedXpert Options menu To run the report manually click on the Samples Comparison command and the window seen in Figure 8 8 below will open In the box marked reference click on the W arrow and a drop down list of all your open sample tables will appear Choose the sample table you would like to compare to In the next box choose the table you would like to compare against Enter the minimum number of exact matches you would like
56. 3 803 ID B 1 1 0ng 5 CO1 ID C 2 0 5ng amp C02 ID C 3 0 5ng 5 C03 ID C 1 O 5ng 7 D01 ID D 2 0 25ng 8 D02 ID D 3 0 25ng 7 D03 ID D 1 0 25ng 3 ID F 2 0 1ng 0 E02 ID F 3 9 E03 ID F 1 1 ID 4 3 1 5ng 6 2 ID Ladder 5 HO3 wv NA 3 1 lgnore Case Also select tables Title 1 0 dilutior Combine eon ia FIGURE 8 14 Combine Sources Window In the example above 1 0ng was entered in the selection filter box All samples containing 1 0ng was highlighted in the box on the left hand side They were then moved to the right hand box using the gt button 1 0 dilution was added as the Title Repeat the steps above to create the different dilution tables for each dilution amount you would like to see on the PHR report Once finished click the X on the upper right hand of the window to close the Combine Sources Window Note Remove the selected samples from the right hand side using the lt button before creating each new sample table 99 ARMEDXPERT USER S MANUAL To run the PHR Report Click on PHR Report and the PHR Report Window will open Click on Add a table button PHR Report O s es Description Table Remove Double click an item to change Add a table selected its description Ok Lancel FIGURE 8 15 PHR Report Window The Select a table Window will open You can either select an entire table or expand the table and select individual sa
57. 334 04 338 05 341 83 D351358 12 13 14 15 16 17 18 19 622 560 592 1278 1177 617 542 490 111 61 115 75 119 66 123 61 127 75 131 95 136 04 140 2 THO1 4 5 6 7 8 9 9 3 10 11 13 3 717 679 816 800 739 638 508 561 480 541 166 04 167 11 171 16 175 2 179 16 183 18 186 24 187 11 191 03 201 83 D135317 8 9 10 11 12 13 14 15 791 637 571 715 564 692 567 681 216 68 220 69 224 63 228 73 232 62 236 52 240 52 244 52 D165539 5 8 9 10 11 12 13 14 15 706 827 795 1350 694 664 573 592 551 252 26 264 2 268 28 272 22 276 33 280 3 284 35 288 42 292 51 D251338 15 16 17 18 19 20 21 22 23 24 25 26 27 28 1032 1144 802 1146 919 987 846 889 766 696 831 309 26 311 64 315 87 320 06 324 29 328 39 332 46 800 639 63 336 48 340 49 344 08 347 64 351 24 354 92 358 7 D195433 9 10 11 12 12 2 13 13 2 14 14 2 15 15 2 16 16 2 1489 1352 1456 1109 1246 1078 1110 929 1081 101 73 105 62 109 49 113 4 115 4 117 35 119 38 911 972 827 856 802 803 121 48 123 34 125 25 927532 129 27 131 29 133 53 135 38 11 12 13 14 15 16 17 18 19 20 21 22 23 24 637 727 748 932 744 1012 1037 868 518 580 629 154 42 158 63 162 74 166 96 170 87 174 91 178 94 918 745 701 182 89 186 82 190 74 194 66 198 49 202 34 206 59 TPOX 6 7 8 9 10 11 12 13 730 732 628 686 620 638 670 463 222 02 225 97 229 9
58. 4 4 below will appear This allows you to manually enter in a sample to your sample table or a new table To enter in a new sample first select source 41 ARMEDXPERT USER S MANUAL type by clicking on arrow next to the Set Sample Source Type box then choose the correct type from the drop down list Set Sample Source Type Identifiler 7 Y filer Profiler COfiler Profiler Plus SEfiler Plus SGM Plus GT Profiler COfiler Identfler Allele s PowerPlex 15 PowerPlex ESI 17 HFU s PowerPlex ESX 17 PowerPlex Y Base Pairs Size Std FIGURE 4 3 set Sample Source Type drop down list Next choose the sample table you would like to add the sample to Click on the V arrow next to the Send to box and choose from the available sample tables opened You also have the option to send it to a new blank table Note The sample table has to be opened to add a new sample to it Enter a sample name Under each Locus type in the allele s RFU s and Base Pairs Use a comma to separate more than one number in each box If you need to enter a new locus click the Add Locus button Once you are finished click the Ok button in the upper right corner of the window The rj button refreshes the list of destination tables i Set Sample Source Type Identifier SendTo Add Table LS jJ Add Lo Loc Locus Loi Allele s Allele s Allele s Allele s Allele s Allele s
59. 418 66 89 1 113 1724 13514 1417 8 11 1212 XY 1213 2226 FIGURE 6 9 Example of a Compare Table Report 64 ARMEDXPERT USER S MANUAL Tab Five Interpretation he Interpretation tab is used to perform interpretation of your samples It has two main sections Mixture Interpretations and Frequency Calculations views Data Oc Checks Match amp Comparison Interpretation Reporting Highlight Lj all ts LE Y Begin Mixture Single Likelihood Probability Mixture Interpretation Source Ratio of Inclusion Mixture Interpretation Frequency Calculations FIGURE 7 1 Interpretation Tab Mixture Interpretation The Mixture Interpretation section is used to interpret mixtures for any selected sample It gives you the information to aide you in making the appropriate calls It lists all available combinations at each Locus based on your settings It keeps track of the proportions for each profile as well as gives you a graphical visualization of the proportions as you choose each combination The Mixture Interpretation tool can be used for a 2 or 3 contributor mixture It allows you to set references and apply stutter when appropriate To start using the Mixture Interpretation Tool click on the Begin Mixture Interpretation command The Mixture Interpretation Window will appear as shown in Figure 7 2 below Shortcut You can also open the Mixture Interpretation window by hol
60. 478 0 1601 0 6719 0 7668 0 5904 0 7211 0 8543 0 8519 0 6947 0 5204 0 8025 0 6647 0 6633 Max 0 9716 0 9185 0 9693 0 9539 1 0000 0 9561 0 8536 0 8348 0 9604 0 9604 0 9639 StdError 0 0885 0 0465 0 1219 0 0678 0 0421 0 0322 0 0480 0 0981 0 0478 0 0854 0 0924 D8S1179 CSF1PO 0351358 0199435 FIGURE 8 20 Example of a PHR Report with hidden Locus 102 ARMEDXPERT USER S MANUAL On the left hand side of the report is the PHR at each Locus The average PHR can be seen at the end of each table as well as the standard deviation the minimum PHR the maximum PHR and the standard error PHR Report D8S1179 D21511 csriPO D351358 0195433 TPOX 018551 Amel 055818 FGA 1 o o o ou o uA e e aae Std Deviation 0 0568 0 0611 0 0707 0 0770 0 0906 0 0334 0 0679 0 1269 0 0788 0 0194 0 0136 Min 0 8652 0 8369 0 8438 0 7841 0 7827 0 9067 0 8093 0 6037 0 7957 0 9246 0 9232 Max 0 9716 0 9629 0 9861 0 9570 0 9667 0 9840 0 9799 0 8561 0 9862 0 9710 0 9592 StdError 0 0254 0 0273 0 0316 0 0344 0 0405 0 0149 0 0304 0 0568 0 0352 0 0087 0 0061_ Std Deviation 0 0183 0 1474 0 0849 0 0143 0 0915 0 0764 0 0328 0 1093 0 0915 0 0699 0 0682 Mi 0 9441 0 6411 0 8333 0 9112 0 7898 0 8045 0
61. 51358 0195433 D18S51 Amel D5S818 FGA The Template Report allows you to create your own customized template report It allows you to create a template in Excel and then incorporate data from ArmedXpert into that customized template 104 ARMEDXPERT USER S MANUAL Hot Keys Function Keys Alt Keys Misc Keys Appendix pu V F2 Clear Match ps D F5 Hide selected Rows or Columns ps nN p5 7 Show Controls FT oo F10 ps M2 Show Alleles F11 Show Base Pairs Alt then right click mouse Increase Column Width Increase Row Height Ctrl Moves to First Row and First Locus Position Home Ctrl End Clears pick via mouse option Shift Moves to Previous Cell in Row Tab 105
62. 6 06 20 E 220159 1 99 lt 999901 15 8F1 Shirt Vict 06 06 20 F gt gt lt 0161 1 G4003F1 06 06 20 H01 cm 2 1 Reag BlankF1 06 06 20 B02 0162 2 Reag BlankF2 06 06 20 C02 20164 99 lt 999901 2 LFS Vict 06 06 20 D02 lt xx0165 04004 04 06 06 20 E02 0166 Reag Blank 06 06 20 F02 lt lt 0167 994 lt 9999K1 11 Blood Vict 06 06 20 G02 20168 39 53333K 2 Blood Susp 06 06 20 H02 0169 QA026 QA 06 06 20 B03 0170 Reag Blank 06 06 20 C03 mw m A A IP ON A CMF Version E gt EMF AS Ignore Case Also select tables CMF Attributes Packet Attributes SOURCELAB DESTINATIONDRI SUBMITBYUSERID SUBMITDATETIME 2011 04 27T1 7 00 41 BATCHID KIT V Save to file Ok Cancel FIGURE 8 11 CMF Window Once you finished selecting the files Choose the CMF Version that is appropriate from the drop down list on the lower left hand side of the CMF window There are four versions The CMF Attributes on the left hand bottom of the window will change based on the version selected Fill in the CMF Attributes CMF Version CMF 3 2 ICMF10 ICMF30 CMF31 B CMF32 DESTITIT FIGURE 8 12 CMF Version Drop Down List 97 ARMEDXPERT USER S MANUAL Click on one of your selected samples The Packet Attributes section on the right hand bottom of the CMF window will appear Click on the arrow next to the SPECIMENCATEGORY box to bring up a list of available categories Select the appropriat
63. 648192 0 012 3 0 0139 0 00123432 0 26488358 0 0291 2 0 0123 0 0246 0 00060516 0 25937629 0 0184 2 29 30 29 30 0 20 0 1843 0 13494488 0 2672 0 1626 0 2906 0 09450312 0 38692578 0 22 0 1738 0 3238 0 11255288 0 40253822 0 248 0 0981 0 3062 0 06007644 0 36609411 0 2021 8 10 0 3005 0 0714 0 0429114 0 35370285 0 185 0 0548 0 02244608 0 32051152 0 125 0 03431552 0 3311 2888 0 165 Unrelated V Full Siblings Parents amp Offspring VY Half Siblings Uncles Nephews VY First Cousins FIGURE 2 32 Frequency Analysis page with Export Menu 29 ARMEDXPERT USER S MANUAL To save a Mixture Interpretation page so that it can be resumed later in ArmedXpert click on Mixture Interpretation The Save As Window will appear You can change file name to whatever name you would like and Save it in whichever folder you would like Click Save once you have the file name and folder chosen mI ArmedX pert Reporting Save in the page data format rem Page Data Import amp Excel Workbook Save as a workbook or in a workbook friendly Format Image Save the entire worksheet or selected range as an image m Frequency Analysis Mixture Interpretation DDA Interpretation Save a frequency analysis page so that it can be resumed later Setup Mixture Interpretation Save mixture interpretation so that it can be
64. 98627 5 2224 22 24 0 1888 0 1378 0 05203328 0 34465832 0 1633 0 10766664 0 07984996 0 225 0 1861 0 083745 0 37371125 0 20555 0 1446475 0 11419625 0 1773 0 1256 0 04453776 0 33685944 0 15145 0 09799388 0 07126582 Caucasian lin 6 2505E 16 1 4661E 6 5 8512E 9 1 7116E 12 1 0258E 14 Black lin 5 2802E418 2 9528E 6 5 5722E 10 2 8928E 13 3 0922E 15 Hispanic T In 2 3643E417 1 8021E 6 1 695E 10 5 4585E 12 3 7263E 14 Caucasian Lin 62 5 Quadrillion 1 46 Million 5 85 Billion 1 71 Trillion 102 Trillion Black Lin 5 28 Quintillion 2 95 Million 55 7 Billion 28 9 Trillion 3 09 Quadrillion Hispanic Lin 236 Quadrilion 1 8 Million 16 9 Billion 5 45 Trillion 372 Trillion 3 Database Size 1 Theta 0 01 Sources o Bl 4 lil gt V Unrelated Y Full Siblings V Parents amp Offspring Y Half Siblings Uncles Nephews First Cousins FIGURE 7 38 Bottom Portion of the Single Source Calculation Page 82 ARMEDXPERT USER S MANUAL The second type of frequency calculations is a Likelihood Ratio calculation It is used to calculate likelihood ratios for mixture samples To obtain the Likelihood Ratio Calculations click on the Likelihood Ratio Button The Select Pick via mouse window will appear Select the sample you wish to perform the calculations on with your mouse ot click on the V arrow and select from the drop down list M ICA via MUUST FIGURE 7 39 Select Pick Via Mouse Window All Loci and the
65. All Stutter applied to all loci will be removed 12 13 1392 2345 n Profile 1 5 Globally tt ronle Mixture Information Only combinations the reference PI are included 12 13 1 For a 2 contributor 2 allele mixture of types AA amp AB 2 2 combination s 12 13 phr 0 52 p 2 0 95 Ref 1 12 12 p 0 05 12 13 phr 1 00 p 0 74 Ref 1 15 13 p 0 26 2 For a 2 contributor 2 allele mixture of types AB amp AB 1 1 combination s 12 13 phr 0 59 Ref 1 12 13 phr 0 59 Ref 1 FIGURE 7 13 Mixture Information after stutter applied 71 ARMEDXPERT USER S MANUAL Making Calls The Mixture Interpretation Window has are three different ways to make profile calls Mixture Interpretation DDA Interpretation Setup Contributor 2 Fick via mouse 0156 1 99 999901115 2F 1 Shirt Vict 06 06 20 D01 Operations References Locus D85173 lt Jo Victim 12 13 P Avg 0 71 PHr 0 40 2 suspect n a P Avg 0 29 ignore Alleles OT 100 Alleles 12 13 Below mPH x 0 00 RFUs 1580 2345 Lock locus BPs 139 55 144 26 hee 12345 1 580 Add Profle 7 100 App ei aba AbD Stuer 0 08 Mixture Information 19 5 Only combinations including the following reference profiles are included 12 13 All combinations have PHr gt 0 4 MPh gt 100 mP gt 0 For a 2 cont
66. E ropes PV ROPA ES EET 12 13 29 30 10 10 12 15 16 6 93 812 12 13 2024 13142 1719 811 1415 X 12 2224 13 28 910 1011 15 693 1 12 9 12 1925 14182 1518 11 1417 XY 912 22225 L1215 30 1412 11 1416 93 1213 12 13 1522 1215 1518 8 13 xv u 20 E MM cJ M ET NN Tc e 13 30 j 10111 1012 1415 8 93 11 1 12 1923 1415 1718 8 1519 n 2324 pee queri FIGURE 3 3 view Samples amp Controls by Allele Q 5 ArmedXpert views Data QCChecks Match amp Comparison Interpretation Reporting yall C Alleles Samples Headings M E M rti ae e e Controls C Base Pairs Indicate clipped data ES Zoom 100 Organize Samples CJ RFUs _ User View Fit Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt ossia onssi orsa csrieo ossis res pass osesse assa possa vun rox onus mit era ere 1218 On Coe E CAO 7 8 6 7 8 22 13 45 8 9 10 5 8 9 15 16 9 10 12 12 6 7 79 19 X Y 7 8 9 17 7 sns 1 s5 ua1 So 116 sinu Teron xv zoo v e amp inn izz 13 14 14 eu 12 XY 12 13 22 puer SS SS EE TE EU 793 12 14 12 118 XY 5 a a a ee eee n ann eee eee izis 39 iniz ii i amp 16 53 1 123 1 amp 22 Tests vem bmi tee emer 3 39 Bonui
67. Locus Allele 1 Allele 2 Allele 1 Allele 1 D851173 12 13 D21511 28 23 30 D75820 3 10 CSFIPO 11 12 maraacn 40 J Save to file Ok Cancel FIGURE 8 13 CMF Window Example Click Ok when you ate ready to save the file The Save As Window will appear In the File Name box type in the appropriate file name Then save it in the appropriate folder Click on the Save button 98 ARMEDXPERT USER S MANUAL PHR Report The PHR Report shows all the peak height ratios PHR as well as the average PHR for selected table s or sample s This is useful to determine thresholds The first step in running your PHR Report is to create dilution tables for the report Import your dilution sample table into ArmedXpert Once your table is imported click on Combine Sources under the Data tab The Combine Sources window will open Type in the selection filter box the dilution you would like to create a table for Click the gt button to move all highlighted samples to the right hand box Type in the title of the table you are creating Click on the Combine button Your new combined sample table will open Combine Sources el Select items to combine Samples amp sources Selected samples amp sources 23 DilutionSeries xls ID B 1 1 0ng 5 0 ID Ladder 1 401 ID B 2 1 0ng 4 B02 ID amp 2 1 5ng 2 402 ID B 3 1 1 403 ID B 3 1 0ng 1 403 ID A 1 15 3 801 ID B 2 1 0ng 4 B02 ID Ladder
68. MT Mixture Interpretation of 0156 1 99999901 15 2F1 Shirt Bring To v Vict 06 06 20 D01 Front Mixture Frequency 0156 1 99 999901 15 2F1 Shirt Vict Bring To 06 06 20 D01 Front v Add Group FIGURE 3 21 Organize Window 40 ARMEDXPERT USER S MANUAL Tab Two Data he Data tab is used to add new samples edit samples combine samples commit samples load samples and sort samples There are three main sections Samples Data Source and Sort Views Data QC Checks Match amp Comparison Interpretation Reporting f zin 5 L Bal o Mew Edit Change Combine Commit Samples Load Samples Connect Disconnect Sort Sample Sample Sources to Data Source From Data Source Server Server Samples Data Source Sort FIGURE 4 1 Data Tab Samples There are four commands in the Samples Section New Sample Edit Sample Change Kit and Combine Sources The New Sample option allows you to enter manually a new sample onto your current sample table ot to a new table The Edit Sample option allows you to edit any sample on your sample table The Change Kit option allows you to change the kit that is shown for the sample Finally the Combine Sources option allows you to combine specific samples or entire tables into one new sample table 3x vp um New Edit Change Combine Sample Sample Sources Samples FIGURE 4 2 Sample Section When you click on New Sample the window shown in Figure
69. N e o o co eq a a D851179 021511 D 7S820 D351358 D251338 195433 vWA Victim 0 81 0 70 0 61 0 65 0 73 0 70 0 71 1 Suspect 0 19 0 30 0 39 0 35 0 27 0 30 0 29 Victim Avg 0 70 Suspect Avg 0 30 d NIN NIN NJ m fpa fee tee eee Calls Mix Int rp s FIGURE 7 26 Proportion Worksheet Tab These reports can be printed and or saved using the print and export features explained in Chapter 2 You can add a comment s to the call report Click on Add Comment button Add a comment to the interpretation report window will open Type the comment you would like to appear on the report Click OK This will add a comment to the Call report below the profiles If you would like to delete the comments click on the arrow next to Add Comment and a Remove Comment button will appear Click on Remove Comment and all comments will be deleted from the report View call report d Add Comment FIGURE 7 27 Add Comment button 78 ARMEDXPERT USER S MANUAL a comment to the interpretation report Ok Cancel FIGURE 7 28 Add Comment to the interpretation report window B M Mixture Interpretation of XX0156 1 99XX999901 15 2F1 Shirt Vict D851179 21511 D75820 CSF1PO 351 12 13 29 30 10 10 10 12 15 10 11 15 Comment made by admin Example of Comment Added Yyndee 4 aayvyvyaqaaani 4e amp FIGURE 7 29 Added Comment Example Popout View call report a calls ce
70. NicheVision Forensics LLC NICHEVISION FORENSICS LLC ArmedXpert User s Manual NicheVision Forensics LLC 526 S Main Street Suite 714G Akron OH 44211 Phone 330 252 2711 Fax 330 252 2713 Table of Contents Chapter 1 Boh Corel oh eid o oet epan itp tere nn ere UNUM pad Mur terre 1 ND EEL 2 LOMO AE E P adaa 3 FRE OTS Ceti SGP idea E N 5 Chapter 2 Armed Xpert Mn p bte Ni 6 A PEE T 7 fuel 25 DOE EE E E E EE 25 s cL 31 gs 32 Chapter 3 Tab One VieWwSs ssssssssisssessssssssssrresesessssssssstrteeeeesssssssstrttteeesssssssssrrreeens 33 N EEE E ET 33 E E E E 37 AOO E 38 lavo a a sm RP 39 QI P e HE 40 Chapter 4 Tab hwo Data tbt 41 O DIE P EET 41 B r Ao tn Rrra oer Tre ern ere 46 91 Chapter 5 Tab Three QC ChecES ooo tetris denen 52 DCA CSC eG e 52 ger 55 OG REVIO N G 59 Chapter 6 Tab Four Match Compatison sss 61 Sam Ie I MI uM 61 T E TET TUE TREE OC 64
71. ODIS QC QC Review Clear FIGURE 5 1 QC Checks Tab Standard Checks There are four types of standard quality control checks They are Check Controls Check Allele Ordering Stutter and RFUs The standard checks will evaluate the samples on your active sample table for quality control issues Click on each type of standard check you wish to perform After clicking each command the check will be performed and your page will display the results of the check All errors will be highlighted A key will be displayed on the page to explain what the highlighted colors mean for the check being done If no errors are found on the check a message will appear at the bottom of the page that states Check completed with no errors as shown below in figure 5 2 Click on the Clear Checks button to clear the check results on the page XX0168 99XX9999K2 Blood Susp 06 06 20 H02 13 13 28 28 9 10 10 11 15 15 6 93 11 12 19 25 14 18 2 15 18 22 2 25 3X0169 QA026 QA 06 06 20 803 130170 Reag Blank 06 06 20 C08 ee 330171 Pos Con 06 06 20 003 1513 3039 joma 1912 iis amp 93 inii iniz 193 mi aie Jas si aul aa 30172 Neg Con 06 06 20 03 pee pe peers pem eere pepe a eee ee Check completed with no errors FIGURE 52 Example of a Check completed with no errors screen 52 ARMEDXPERT USER S MANUAL Description of each standard check and examples of each check results are shown below The ke
72. Stains Review Ext My Recent DEMO_I_2 Component_Vict_and_5 Susps_D3_Extra_Allele txt Documents DEMO_I_3 Component_No_Controls_ _51_52_E txt My Documents My Computer File name My Network Files of type FIGURE 2 26 Open Window Q pF ArmedXpert CJ Views Data QC Checks Match amp Comparison Interpretation Reporting All Alleles 2 Samples 4 F Headings Controls C Base Pairs C All 3N Indicate clipped data Es Zoom 100 _ Samples _ RFUs User DEMO_I_2 Component_4_Vict_Mix_Stains_Analyst txt Ladder Identifiler 06 06 20 A01 819 10 24 25 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 8 9 15 16 17 9 10 11 12 11 12 13 6 7 7 9 10 Ladder Identifiler 06 06 20 A03 Ladder Identifiler 06 06 20 F03 8 9 10 24 24 2 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 8 9 15 16 17 9 10 11 12 11 12 13 6 7 7 9 10 XY 7 89 17 18 19 20 XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 H02 13 13 28 28 9 10 10 11 15 15 6 93 1 12 9 12 1925 14182 1518 1 1 1417 XY 912 22225 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 12 13 128 29 30 9 10 110 11 12 15 16 6 9 3 8 11 12 9 12 13119 20 24 25 13 14 142 18 215 17 18 19 8 11 14 15 17 XY 9 12 22 222 24 25 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 01 12 13 28 29 30 9 10 110 11 12 15 16 6 9 3 8 11 1219 12 13 19
73. able the feature Once checked all alleles below the prescribed mPH s will not be considered in the mixture combinations and show as grayed out As shown in the example below Allele 16 has a value of 171 which is below the 175 setting for mPH Allele 16 has been grayed out and all combinations including Allele 16 are excluded from the possible mathematical options shown in the mixture interpretation results Locus 1178 5 gt Profile 1 n a Profile 2 n a 5 Alleles 3 11 12 13 16 Profile 3 n a RFUs 458 3002 277 2381 171 BPs 126 95 135 2 133 47 143 97 157 13 View call report x Add Comment Popout calls 100 5 Ja 12981 Appl Globally Apply Stutter Profle 1 0 08 Mixture Information All combinations have PHr gt 0 3 MPH gt 175 mP gt 0 1 For a 3 contributor 4 allele mixture of types AA AC amp BD 3 12 combination s pp E ue S n Me ha v 11 1 7 f nhr A SRS 2M 1 nhre NAA nm 6011 211 n fi FIGURE 7 11 Remove Alleles Below mPH Feature 70 ARMEDXPERT USER S MANUAL Apply Stutter The Mixture Interpretation tool also includes features to apply stutter You can either apply stutter to an individual locus or apply it globally to all loci To apply stutter to just the individual locus click on the Apply Stutter button If you wish to apply stutter to all loci click on the box next to Apply Globally
74. ag BlankF2 06 06 20 C02 SS eene ien d EEE ee si tr esum eus ruens XX0164 99XX9999Q1 2 LFS Vict 06 06 20 D02 a 4 0 XX0165 QA004 QA 06 06 20 E02 912 9 30 14 298 12 14 17 20 2 2 0 XX0166 Reag Blank 06 06 20 F02 Be ee Bea E Be os BS ee RET A eS ee eee XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 8 12 1213 2024 13 142 1719 8 11 1415 X 22 24 4 1 3 16 16 0 XX0169 QA026 QA 06 06 20 B03 11 12 8 13 NEUE 911 2 3 2 1 XX0170 Reag Blank 06 06 20 003 Zz ie E eee a ee ees eee XX0171 Pos Con 06 06 20 D03 10 11 1 10 12 14 15 8 9 3 3933 312538 8 1819 11 2834 2 1 1 XX0172 Neg Con 06 06 20 E03 U GERE Saas 1 0 1 oooo oo 0c oa DEMO 1 2 Component 4 Vict Mix Stains Analyst txt 75820 CSFIPO D351358 THO1 D135317 D165539 D251338 D195433 vWA TPOX D18S51 AmellDSS818 FGA Ix Y Y Ladder Identifiler 06 06 20 A01 0 9 Ladder Identifiler 06 06 20 A03 0 0 0 Ladder Identifiler 06 06 20 F03 0 0 o XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 H02 9 10 10 11 15 16 93 11 12 9 12 19 25 14 182 15 18 5 11 14 17 XY 9 12 222 25 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 001 6 9 3 0 0 0 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 E01 0 0 0 XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 FO1 6 9 3 0 0 0 XX0161 1 QA003F1 06 06 20 H01 0 0 0 XX0162 1 Reag BlankF1 06 06 20 B02 0 0 XX0162 2 Reag BlankF2 06 06 20 602
75. ailable if you have administration capabilities It has two tabs Users and Database a x ArmedXpert Administration Users Database New Edit Delete User User User User Administration User Name First Name Last Name DateCreated admin 10 22 2010 1 38 PM FIGURE 2 21 ArmedXpert Administration User Window The first tab Users allows you to enter a new user edit a user and delete a user Enter a New User click on the New User command The New User box will open Type in the appropriate information Only the User Name and Role are required to be filled in There is a drop down list for the Role options Click on the arrow and select the appropriate role Click on Ok once finished 22 ARMEDXPERT USER S MANUAL Hew User ca 5 User Name First N ame Last Name Password Confirm Password Role User M Ok Cancel FIGURE 2 22 New User window To Edit a User click on the user name you would like to edit Then click on the Edit User command The user information will be filled in Change or add any information that is appropriate for that user To change a passwotd enter the current login password and click on Reset password Type in New Password and confirm it in the next box Click on Ok dit User aj LAJ User Name First Name Last Name Password Reset password New Password Confirm passw
76. all of the information from a sample table It lists the alleles RFU values and Pair Pairs values for each sample on the sample table by Locus To run the report have the sample table that you want to see the total data for open and in front of all open windows and click on the Total Data button DEMO_I_2 Component_4_Vict_Mix_Stains_Analyst txt c3 5 x 4 Vict Mix Stains Analyst Locus Alleles Base Pairs Ladder Identifiler 06 06 20 F03 D851179 8 9 10 11 12 13 14 15 16 17 18 19 1505 1372 1682 1830 1043 955 960 1370 931 887 123 04 127 11 131 15 135 31 139 48 144 13 148 63 1043 781 153 03 157 26 161 48 165 56 169 62 021511 24 24 2 25 26 27 28 28 2 29 29 2 30 30 2 31 31 2 2601 1111 1946 1448 1698 2501 1792 1890 1211 184 64 186 68 188 64 192 56 196 46 200 37 202 27 32 32 2 33 33 2 34 34 2 35 35 2 36 37 38 1162 1224 1150 1194 1574 1114 1199 1436 1082 204 24 206 22 208 2 210 19 212 18 214 1 216 09 1415 1743 1132 1626 2386 867 218 09 220 09 222 02 224 11 225 97 228 06 230 232 02 236 239 91 D7S820 6 7 8 9 10 11 12 13 14 15 1315 1115 1257 1310 1198 845 1002 1142 1103 255 16 259 14 263 2 267 2 271 3 275 32 279 44 1017 283 49 287 56 291 64 CSF1PO 6 7 8 9 10 11 12 13 14 15 1066 1657 1479 1568 1030 1690 1083 727 1209 304 72 309 04 313 3 317 6 321 78 325 91 329 99 1448
77. ations have PHr gt 0 3 mPH gt 175 mP gt 0 12 1580 13 2345 Only combinations including the following reference profiles are included 29 30 All combinations have PHr gt 0 3 mPH gt 175 mP gt 0 28 916 29 1144 30 994 For a 2 contributor 3 allele mixture of types AA amp BC 1 3 combination s 29 0 87 B D 0 2 f For a 2 contributor 3 allele mixture of types AB amp BC 2 3 combination s Only combinations including the following reference profiles are included 13 14 2 All combinations have PHr gt 0 3 mPH gt 175 mP gt 0 13 787 14 410 14 2 886 18 2 292 For a 2 contributor 4 allele mixture of types AC amp BD 1 3 combination s 13 14 2 phr 0 89 p 0 70 14 18 2 phr 0 71 p 0 30 LE 37 Only combinations including the following reference profiles are included 10 10 38 combinations have PHr gt 0 3 mPH gt 175 mP gt 0 4 Calls Mix Interp Proportions FIGURE 7 25 Mix Interp Worksheet Tab 77 ARMEDXPERT USER S MANUAL The Proportions worksheet tab shows the proportion percentages by Locus and the overall average for each Profile As well the same graph that appears on the Calls tab is shown on this tab NOTE Only the Loci that has been called will appear in this view Mixture Interpretation of XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 c3 es B Victim B Suspect o oo
78. bsection is Frequencies options The top half of the screen allows you to choose which frequency tables are enabled for frequency calculations A check indicates that table is enabled for the frequency reports Click on the box next to each option to add or remove a check in the box The Theta amount can be changed here by clicking in cell next to each race in the Theta column and typing a new Theta value The bottom half of the screen allows you to choose the source of frequency ratios the system will be use Arme dX pert Options c3 5 General Mixture Interpretation Quality Control Frequencies Frequency Options Data Frequency options intem etatior Quality Control Enabled Caucasian Black Admin Console Hispanic Reporting Description Enabled Delete Races Delete Sources Add Frequencies Review Frequencies Ok Cancel FIGURE 2 12 Frequency Options Clicking on Delete Races or Delete Sources will permanently delete a Race or Source Make sure you actually would like to delete them before clicking on either of these buttons 13 ARMEDXPERT USER S MANUAL Click on the Add Frequencies button to add Frequencies Begin by typing in the source name Then manually enter in new frequencies paste in frequencies or import frequencies from a file To manually enter in frequencies Start at the first box under the Race column Enter in the correct
79. cally saved and opened without manual typing There is no limit on number of population frequencies that can be stored Automated Sample vs Database results Total Data and CODIS Reports Disposition Forms Checks Samples and Statistics fit single page format Mixture Interpretation ARMEDXPERT USER S MANUAL Signing into ArmedXpert When you start ArmedXpert the Login window will open Enter your User name and password Default user name is admin with no password Click the Ok button Note When you initially start ArmedXpert the first time and until you register the Registration Dialog window will open before the Login window To clear your login in history click on the Clear login history button Login User Name Password Clear login history Cancel Options gt gt FIGURE 1 3 Login Window To change the database that ArmedXpert is connected to click on the Options gt gt button The Connection Properties tab opens Change from the available databases by clicking on the W next to Connection name box to choose between the available Connection Names Login Connection N ame Connection Type Connection String Login Connection Properties SQLite SQLite data source Armedpert db Save this Configuration Delete this Configuration Ok Cancel Options lt lt FIGURE 1 4 Connection Properties ARMEDXPERT
80. ce you have selected the correct sample s or table click on the Delete button A confirmation window will appear to confirm you want to delete Click Ok to confirm the delete 49 ARMEDXPERT USER S MANUAL Datasource Load css Selected samples amp sources V Ignore Case Also select tables Search Search by name V Search Tables Search Profiles Delete Load FIGURE 4 17 Deleting a Datasource sample To connect or disconnect from the server click on the appropriate command When you click on the Connect Server button the Login window appears This enables you to change between databases while in ArmedXpert The Login window instructions are on page 3 Connect Disconnect Server Server FIGURE 4 18 Connect Disconnect Server buttons 50 ARMEDXPERT USER S MANUAL Sort The final section is the Sort section Click on the Sort button and the Sort Window opens as shown in Figure 4 20 below Select the key s to sort by Then click on the gt button to bring over one key keys columns on the spreadsheet The gt gt button is used if selecting all the keys The keys selected will then appear in the box to the right You can change to Sort Order from Descending to Ascending by clicking on the Sort Order box and choosing from the drop down list the applicable choice You can also sort normally or text as numbers by choosing under the drop down list in the Sort As column Once f
81. ces c3 s Select items to combine Samples amp sources Selected samples amp sources H DEMO 2 Component 4 Vict Mix Stains Analyst txt 220156 1 33 5583831 15 2F1 Shirt Vict 06 06 20 D01 DEMO_ _2 Component_4 Vict Mix Stains Review txt E 220159 1 99 lt 999901 15 8F1 Shirt Vict 06 06 20 F01 Ladder Identifiler 06 06 20 F03 Fs 240156 3 99 999901 15 2F3 Shirt Vict 06 06 20 E01 Ladder Identifiler 06 06 20 403 p Ladder Identifiler 06 06 20 401 e 220156 1 996999901 15 2F1 Shirt Vict 06 06 20 0 7 280156 3 99299990 1 15 2F3 Shirt Vict 06 06 20 E gt gt 220159 1 3326433335 1 15 8 1 Shirt Vict 06 06 20 F _ 220161 1 QA003F1 06 06 20 H01 220162 1 Reag BlankF1 06 06 20 B02 XX0162 2 Reag BlankF2 06 06 20 C02 XX0164 93 54999301 2 LFS Vict 06 06 20 D02 XX0165 Q OD4 QA 06 05 20 E02 lt lt 20166 Reag Blank 06 06 20 F02 7 99 9999K1 11 Blood Vict 06 06 20 G02 20168 99 6 lt 9999K2 Blood Susp 06 06 20 H02 280169 04026 QA 06 06 20 B03 lt 0170 Reag Blank 06 06 20 C03 S liil E 4 lgnore Case Also select tables Title 156 1 33 253333 1 15 2F1 Shirt Vict 06 06 20 D01 lt 0159 1 33433330 1 15 SF1 Shirt Vict 06 06 20 FUT x Combine FIGURE 4 8 Combine Sources Window A new combined sample table will appear with the default or user defined title An example is shown in Figure 4 9 below In this example the three samples selected above
82. creen 55 ARMEDXPERT USER S MANUAL The CODIS Alleles command checks the alleles at each locus against CODIS formatting rules It highlights in pink any sample s locus that contains alleles not accepted by CODIS Q Us ArmedXpert eM Views Data QC Checks Match amp Comparison Interpretation Reporting amp ll ATE x Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Alleles Count X muli Review Checks Standard Checks CODISQC QCReview Clear C Program Files NicheVision Inc ArmedXpert data Saved_Profiles Page_Data MT Test D T txt c 01 Identifiler Kit Standard 10 10 0401 la ola Mixture 0 Nest 10 10 04 Mix Mixture 1 1 10 10 04 Mix 10 1 12 1 12 17 18 20 25 26 12 143 15 16 17 18 19 M 18 1 12 19 20 24 Mixture M F 1 10 10 10 04 2 Mix 19 11 12 11 12 17 18 20 25 26 12 14 16 17 18 19 14 18 10 11 12 Mixture 1 100 10 10 04 2 Mix ost aziz ms masse masus sn Mixture M F 1 4 10 10 04 Mix 1 12 11 12 17 18 20 26 12 142 15 16 14 18 11 12 19 20 24 Mixture_M_F_10_1 10 10 04 2 Mix Mixture_M_F_100_1 10 10 04 2 Mix Mixture M F 4 1 10 10 04 Mix 19 FIGURE 5 9 CODIS Alleles Result Example The Allele Count command highlights in pink any sample s locus that contains a number of alleles above the user defined amount An Allele Count window appears aft
83. ding down the alt button and clicking on any of the Loci or the sample name for the sample you wish to interpret The Mixture Interpretation window for that select sample at that locus will open If you are on the sample name the first locus is displayed This enables you to skip the select sample step below 65 ARMEDXPERT USER S MANUAL Mixture Interpretation DDA Interpretation Oo 5 ey Setup Pick via mouse Operations References Highest to Locus s gt PH 0 40 gt ial ES Lowest gt Ignore Alleles en Alleles Below mPH m k 0 00 RFUs Lock locus BPs Popout View call report calls Add Comment TRIE Apply IT Globally PPY stutte No sample selected 0 0 Mixture Information 12 3 FIGURE 7 2 Mixture Interpretation Window Select Sample perform mixture interpretations you must fitst select the sample you would like to interpret The pick via mouse option will allow you to select the sample using your mouse Clicking the V arrow will provide a drop down list of all available samples from which you can select the appropriate sample Setup Fuck via mouse T FIGURE 7 3 Select Pick via mouse section 66 ARMEDXPERT USER S MANUAL Once the sample is selected the information will fill into the Mixture Interpretation Window The selected sample and current Locus will be highlighted on the sample
84. dow Note All profile names can be manually renamed in the Mixture Interpretation Window regardless of these settings 11 ARMEDXPERT USER S MANUAL The second subsection is the Interpretation Quality Control options This section displays the thresholds used during the check controls These can be changed by each locus Click in the cell you would like to change and type a new amount You can also add a MPH minimum peak height or PHR Range peak height ratio for each separate locus This will supersede the default for each set in previous Mixture Interpretation tab You can do that by just typing in the appropriate number for each If no amount is filled in the MPH or PHR Ranges the default values will be used You can add Loci from a sample table by clicking on the Add Loci from Table button The sample table needs to be opened in ArmedXpert The Delete Selected button is used to delete the item selected ArmedXpert Options c I s General _ Mixture Interpretation Quality Control Frequencies Frequency Options Interpretation quality control options Quality Control Identifier Stutter PHR Ranges 0 08 D21511 0 03 D75820 0 08 CSFIPO 0 09 D351358 THOT 0 05 0138317 0 08 D165539 01 251338 011 D195433 013 AMA 013 TPOX 0 06 D18551 017 Reporting Admin Console FIGURE 2 11 Interpretation Quality Control Options 12 ARMEDXPERT USER S MANUAL The third su
85. e Remove Comments FIGURE 7 30 Remove Comments Button As you make each call the proportion average will be shown on the Mixture Interpretation Window It is located next to the Highest to Lowest feature Mixture Interpretation Interpretation Setup Pick via mouse 220156 1 994699990 1 15 2F1 Shirt Vict 06 06 20 D01 i Operations z Contributor 2 t References 7 Locus D351358 2 a Jo Highestto sus 15 16 P Avg 0 69 PHr03 5 Lowest 5 Poe Profile 2 15 P Avg 0 31 k Remove Mleles 175 7 HT 100 2 Alleles 15 15 elow m E m RFUs 1548 747 FIGURE 7 31 Proportion Average Section 79 ARMEDXPERT USER S MANUAL There is an Operations button to the right of the selected sample This opens a mixture frequency report for this sample or allows you to open an OSIRIS Plot File or Virtual Electropherogram Click on the Operations button and a drop down list will open open the Mixture frequency report click on Mixture Frequency The Locus you currently are on will be shown You can choose between Modified RMP and Restricted RMP If you would like to have the report shown all Loci with Restricted RMP click on the box next to Apply Restricted Globally If you do not all Loci will have the Modified RMP applied Make sure the Contributor number is correct Finally click the Open Frequency Report button A mixture frequency report will then open to aide you with making your cal
86. e Once set the mixture interpretation window will only show allele possibilities equal or greater than the value set For example if the minimum peak height mPH is set at 75 as shown below the Mixture Interpretation Window will only include allele possibilities equal to or greater than 75 mPH The sort loci by allele count option displays the loci with the largest number of alleles first in the Mixture Interpretation Window It is the same as checking Highest to Lowest in the Mixture Interpretation Window The List proportions as dilutions 1 X option displays the proportion as dilutions instead of percentages in the mixture interpretation window Click on the box next to each to turn these features on or off Note The mixture interpretations settings can be changed in the Mixture Interpretation Window for a specific sample This does not change the default settings for the software The default settings can only be set through this window ArmedXpert Options ca 5 General Mixture Interpretation Quality Control Frequencies Frequency Options Data Mixture interpretation settings nterpretatior 5 Quality Control Default peak height ratio PHr I TO aii as dilutions rad Reporting Default minimum peak height mPH Admin Console Default homozygote threshold HT Default minimum proporition mF 0 Prompt to save on window close Default stutter 7z 100 7 Prompt to save on application close Sort loci
87. e Source XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 _ Frequency Frequency ian packing Heddency Parents and Half Sibs diss eie Unrelated Full Siblings First Cousins Locus Locus Profile Allele 1 Allele 2 Offspring Uncles Neph s D851179 1 12 3 12 13 0 1811 0 2321 0 08406662 0 374316655 0 2066 0 14533331 0 114699965 0 1899 0 1788 0 06790824 0 35915206 0 18435 0 12612912 0 09701868 0 3301 0 13494488 0 41736122 0 26725 0 20109744 0 16802116 0 2906 0 2906 0 086509876 0 41641209 0 2906 0 18752418 0 13598627 0 3238 0 3238 0 107035976 0 43811161 0 3238 0 21432322 0 15958483 0 3062 0 3062 0 095882856 0 42653961 0 3062 0 19997922 0 14686883 0 2537 0 3251 0 16495574 0 435938935 0 2894 0 22717787 0 196066805 0 2714 0 16284 0 43356 0 2857 0 22427 0 193555 0 2536 0 3923 0 19897456 0 46121864 0 32295 0 26096228 22996842 551355 1 1 1 1 15 6 15 16 n n a n 12240772 n A176n1 R71 n l n 2022034 n1 7nansa l V Unrelated Full Siblings Parents amp Offspring Half Siblings Uncles Nephews V First Cousins FIGURE 7 37 Single Source Calculation Page U 3539 5555 VU L2Z 3531 55 V 495Z015ULZ U 3539 U 2Z3595 Z0U5 U 10524005 9Ud 0 3556 0 3556 0 128742846 0 45941284 0 3556 0 24102568 18373852 0 2906 0 2906 0 086509876 0 41641209 0 2906 0 18752418 135
88. e appropriate allele and across from the right profile a drop down list will appear as seen in Figure 7 20 below You can then click on the appropriate choice You can also delete an allele using the drop down list Once chosen you can move to a different Locus using the lt or gt arrows next to the Locus to move forward ot backward or click on the V arrow on the Locus box to move to a specific locus You can close this window by clicking on the X in the upper right hand corner Mixture Interpretation of XX0156 1 99XX999901 15 2F1 gt D851179 2 Reset 12 13 1580 2245 Profile 1 J J 3 Profile 2 FIGURE 7 19 Calls made from Popout Calls Window 74 ARMEDXPERT USER S MANUAL D851178 2 Lej ba Ree 12 13 1580 2345 Profile 1 J Profile 2 y Remo ve Allele FIGURE 7 20 Choosing Any in the Popout Calls Window NOTE Once you make a call with any of the methods it will be recorded in all the methods Therefore you can change methods throughout the mixture interpretation without losing any calls already made using one of the other methods For example once 12 13 were called for Profile 1 it was highlighted blue in Method One and check marks were recorded marking alleles 12 13 in Methods 2 and 3 Navigation There are a few different ways to navigate to different Locus in the Mixture Interpretation Window You can use the method explained above in the Popout ca
89. e are three different ways You can click on the Prev or Next buttons you can type a number directly in the number box to go directly to a certain number or click the up and down arrow buttons next to the number box Review I mew 1 ilz FIGURE 5 17 QC Review Moving Tools Move to each highlighted result to obtain information about it Each highlighted result is at a specific sample s locus which will be outlined on the sample table when reviewing the result Some of results have fixes that can be done such as remove an allele during the stutter check review as shown below in Figure 5 18 Click on any fix that you would like to have done Once you ate finished with QC review window click on the Done button 59 ARMEDXPERT Review s 2s OC Review USER S MANUAL S7 Ged lie n oin D13531 Alleles RFUs 10 200 11 4688 6 00 10 200 is 4 266 of 11 4688 10 could be stutter Remove allele FIGURE 5 18 Example of QC Review Window for the Stutter Check CA 5 Prev Next 1 i 486 D 75820 Alleles AFUs 8 362 3 201 10 253 12 334 a 3 201 is below scale 10 253 is below scale T FIGURE 5 19 Example of QC Review Window for the RFU Check OC Review c3 51 3 Prev Next 3j eee The peak height ratio of 10 200 to 11 4588 is 0 04 which is less than 0 5 Done FIGURE 5 20 Example of QC Review W
90. e category and fill in the rest of the appropriate information for the sample If you scroll down through the Packet Attributes you will see all of the Loci and the Allele s at each Locus All checked Loci will be included Proceed to the next sample until finished with all Samples CMF Samples amp sources Ladder Identifiler 06 06 20 F03 Ladder Identifiler 06 06 20 403 Ladder Identifier 06 06 20 401 280156 1 99999901 15 2F1 Shirt Vict 06 06 20 7 290156 3 99 lt 99990 1 15 2F3 Shirt Vict 06 06 20 E 153 1 99 lt 999901 15 8F1 Shirt Vict 06 06 20 F 220161 1 GA003F1 06 06 20 H01 1 Reag BlankF1 06 06 20 B02 201622 Reag BlankF2 06 06 20 C02 20164 99 999901 2 LFS Vict 06 06 20 D02 220165 O4004 QA 06 06 20 E02 0166 Reag Blank 06 06 20 F02 0157 994 lt 9999K1 11 Blood Vict 06 06 20 G02 20168 33 543333K2 Blood Susp 06 06 20 H02 280169 04026 QA 06 06 20 B03 CMF Version ii gt CMF 3 2 CMF Attributes SOURCELAB DESTINATIONORI SUBMITBYUSERID SUBMITDATETIME 2011 12 09T15 02 41 BATCHID KIT DEMO_I_2 Component_4_Vict_Mix_Stains_Analyst txt XX0170 Reag Blank 06 06 20 C03 al Selected samples amp sources 2 0156 1 99 999901 15 2F1 Shirt Vict 06 06 20 D01 4 Ignore Case Also select tables acket Attributes SPECIMENCATEGORY Convicted Offender SOURCEID CASEID PARTIAL SPECIMENCOMMENT SPECIMENID 0156 1
91. ection in ArmedXpert Options Menu It highlights in yellow any sample s locus that contains RFUs that are off scale Q ArmedXpert Views Data QC Checks Match amp Comparison Interpretation Reporting J Er m PE Y od Ae 7 05H99 aw 9 Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Alleles Count x multi Review Checks Standard Checks ji CODIS QC QCReview Clear DEMO 2 Component 4 Vict Mix Stains Analyst txt ee es A91 7 9 1 D851179 D21511 75820 CSF1PO D351358 THO1 D135317 8 9 10 24 25 67 8 67 89 12 13 4 5 8 9 10 eau 15 16 17 9 10 11 12 11 12 13 6 7 15 16 17 9 10 11 12 11 13 13 8 9 10 24 242 6 7 8 6 7 8 9 12 13 4 5 8 9 10 6 7 7 9 1 402 13 13 28 28 9 10 10 11 15 15 6 93 11 12 11 11 14 1 DO1 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 114 15 5 D165539 5 8 9 5 89 5 8 9 12 13 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 1 12 13 28 29 30 9 10 10 11 12 15 16 6 93 8 11 12 9 12 13 8 11 14 15 13 14 28 311 11 12 10 10
92. elect from the drop down list The Mixture Frequency page will display All Loci and their corresponding alleles are imported into the page On the left half of the screen 1s each locus with each corresponding allele s with the calculations for each allele by ethnicity On the right half of the screen you can see the calculation of any allele combination At the first allele click the arrow next to Add Combo box to bring down the available allele combination drop down list Select the appropriate combination and ArmedXpert will automatically fill in the calculations for that allele combination The drop down list gives you all possible combinations as well as RMP Random Match Probability and 2p options as well as a remove locus option Note If a RMP or 2p option is chosen no other combinations can be added for that Locus When completed the Add Combo box will appear in the next column Add all combinations that are necessary to perform the calculation Continue this process for each locus listed Note The Sum of column will change with each added combination uixture Frequency XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 T XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 Locus Locus Profile Allele 1 Allele 3 Allele 4 H D851179 12 13 12 13 T D21511 28 29 30 28 24 539 D75820 Low CSF1PO 10 11 12 ao 11 am 0351358 15 16 _as T
93. er clicking on the Allele Count command Enter the maximum number of alleles that is accepted and click Ok Allele Count c3 5 Maximum number of alleles Ok Cancel FIGURE 5 10 Allele Count Window Qo hd ArmedX pert Views Data QC Checks Match amp Comparison Interpretation Reporting amp x amp HR B z amp W a x Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Alleles Count x multi Review Checks Standard Checks CODIS QC QCReview Clear DEMO 2 Component 4 Vict Mix Stains Analyst txt D1G95 9 DISISS 9198433 vwA MM EMEN E XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 H02 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 060620001 12 13 MM 12 15 16 6 9 3 8 1 5 17 18 19 8 11 14 XY 2 15 16 18 931 32 1 28 293 1213 i 8 20 12 13 29 30 10 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 01 XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 F01 XX0164 99XX9999Q1 2 LFS Vict 06 06 20 D02 XX0167 99XX9999K1 11 Blood Vict 06 06 20 G02 8 12 12 13 13 14 2 17 19 8 11 14 9 12 5 25 14 18 2 15 18 11 11 14 17 FIGURE 5 11 Allele Count Result Example ARMEDXPERT USER S MANUAL The Check X command checks for samples that only have a single X dis
94. ert Options Exit Armedxpert FIGURE 1 2 ArmedXpert Menu ARMEDXPERT USER S MANUAL ArmedXpert Specifications Compatibility Supports Windows 7 Vista XP 32bit or 64bit Imports ABI Genemapper ID or ID X Genemapper or Data Files GeneScan Tables Imports OSIRIS oer projects Excel CSV or XML formats Passwotd Protected with User roles in Microsoft SQL 32bit or Database 64bit Easily manage multiple databases Combine tables quickly with advanced sorting QC Checks and Matching Gea adage Cheda Control Checks Allele Ordering Max Stutter Low Scale and Off Scale RFUs CODIS QC Allele formatting Allele count Check X Off Scale Data Peak Height Ratio Find Where Reference is Included Find Included to the Reference Find Foreign to Reference Find Where Reference Includes Foreign List Foreign Alleles Combine References and compare tables instantly Interpretation Displays all possible combinations for 2 or 2 contributor scenarios Flags possible stochastic effects Allows for peak height ratio thresholds minimum RFUs minimum proportions and max stutter per locus Audit trail real time proportion charting and sort for possible DNA degradation Random Match Probability RMP Modified Restricted amp Unrestricted Likelihood Ratios Restricted and Unrestricted Statist CPE CPI RMNB and Single Source with relatedness All stats tatistics A can be generated automati
95. etation to the profile name With the Sample Name you can customize when the sample name starts and how many characters are used in the profile name The right hand side is for custom naming To enable Custom Naming place a check mark in the box next to Custom Naming and turn off all check marks on the left hand side Enter a name in each profile box These profile names will appear each time the mixture interpretation window is opened The Swap 3P order allows the third profile to swap to the second position in a three person mixture This enables an elimination profile to move ahead of the suspect profile when applying references see note below Note References are added in order of profiles when using the auto call reference feature in the Mixture Interpretation Window For example the first reference is added to the first profile and the second reference is added to the second profile So make sure you keep this in mind when assigning custom naming ee c9 c9 1 9 c9 9 c9 9o 9 Profile naming scheme J Text Profile Custom Naming Auto number Sample Name 1 gt Date Time Preview Profile 01 Custom Naming 1 Victim Ok Cancel 2 Suspect 3 Elimination FIGURE 2 9 Profile naming scheme Window Profile 38 9 16 Profile 38 12 14 Profile 40 11 13 ictim 9 16 Elimination 12 14 Suspect 11 13 FI GURE EN 0 Examples of what the Profiles look like in the Mrxture Interpretation Win
96. ew Frequencies window Just type in the appropriate cell the change Once you have made your changes click on the Commit button 14 ARMEDXPERT USER S MANUAL The fourth subsection is additional Frequency Options The top section allows you to set the single source frequency calculation database size and gives you a choice to either use Theta or not The middle section allows you the option to use Theta or not for the mixture frequency calculation It also gives you an option to include Locus Detail in the Mixture Frequency calculation report The bottom section allows you set the number of significant digits displayed in the frequency calculations ArmedX pert Options 2 pl x General Mixture Interpretation Quality Control Frequencies Frequency Options Data Single source frequency calculation options Juality Control Database Size 1 J Use theta Reporting Admin Console Mixture frequency calculation options J Use theta Include Locus Details in Report General frequency options No significant digits No significant digits English Ok Cancel FIGURE 2 14 Frequency Options window 15 ARMEDXPERT USER S MANUAL The fourth main section is the Quality Control section This section has two subsections Options and Ladder and Controls The Quality Control settings are used during the Quality Control Checks The Quality Control Checks are discussed in Chapter 5 The first
97. ff profiles to the Database so they can be retrieved for use later to compate against or commit a ladder and control check table to the Database Any sample or table can be saved or committed to your database Connect and Disconnect Server commands allow you to connect or disconnect to the server 9 39 Commit Samples Load Samples Connect Disconnect to Data Source From Data Source Server Server Data Source FIGURE 4 10 Data Source Section To commit a sample from a sample table as a table click on the sample table so it is open and active on the screen Click on the Commit Samples to Data Source button The Datasource Commit window will appear Click on the next to the Sample Table and all the samples on the sample table will appear on the left hand side of the window Click on the sample or table you wish to commit click the gt button and the sample or table will be move to the box on the right Repeat this for as many samples as you wish to commit QQ Datasource Commit Select items to commit to the datasource 8 Ladder and Cortos EFE E Ladder and Controls 4 Ignore Case Also select tables Commit items as Table Individual Profiles Table Name Ladder and Controls Type kit Identifiler Commit Done FIGURE 4 11 Datasource Commit Table Window 46 ARMEDXPERT USER S MANUAL You can also use the Selection Filter box to enter in a specific name and all samples containing
98. fit just the Sample amounts 37 ARMEDXPERT USER S MANUAL Zoom The Zoom section allows you to change to size the page is displayed in Click the Zoom button to bring up the Zoom window which allows you to change to a specific size Click on 100 to change it back to original size S od Zoom 100 Zoom FIGURE 3 15 Zoom Section 200 100 752 50 25 Lusto EA Type in user defined percentage Ok Cancel FIGURE 3 16 Zoom Window 38 ARMEDXPERT USER S MANUAL Show Hide The Show Hide section allows headings and clipped data to either be shown or hidden The Headings option is for viewing the headings for each column You can change the size of the column to a user defined width by displaying the headers and using your mouse to change the size of a specific column The Indicate clipped data option outlines in red all cells that are not shown completely so you know there is more information in that cell If a check mark is in the box next to either option it means headings and or clipped data will be shown If no check mark is shown headings and or clipped data will be not be shown Headings Indicate clipped data Show Hide FIGURE 3 17 Show Hide Section Q gt ArmedXpert views Data ma QC Checks Match amp Compa Reporting a All Alleles Samples R V Headings um Controls C Base Pairs All Indicate clipped da Z Zoo
99. hat are included in the reference All loci that contain an exact match to the reference are highlighted orange All loci that contain the part of the reference and no other allele s are highlighted yellow FIGURE 6 5 Find Included in the Reference Example Find Foreign to Reference is used to find loci that contain allele s that do not match the reference All loci that contain allele s that do match the reference are highlighted red DEMO_I_2 Component_4_Vict_Mix_Stains_Analyst txt 075820 CSF1PO D351358 THO1 D135317 D165539 D251338 D195433 vwa TPOX 18551 Amel D5S818 Ladder Identifiler 06 06 20 A01 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 89 15 16 17 9 10 11 12 11 12 13 6 7 1 0 Ladder Identifiler 06 06 20 A03 4 5 8 9 10 5 8 9 15 16 17 9 10 11 12 11 12 13 6 7 1 1 0 Ladder Identifiler 06 06 20 F03 4 5 5 8 9 7 7 9 10 1 1 0 XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 02 16 16 0 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 4 4 0 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 E01 4 4 0 XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 F01 4 4 0 XX0161 1 QA003F1 06 06 20 H01 11 12 1418 6 89 11 13 17524 1514 1417 811 12 3 1 2 XX0162 1 Reag BlankF1 06 06 20 B02 icon ee ee XX0162 2 Re
100. he FORENSIEINDEK E urnon moemoe momoo en exansesounceiowoves tewen ED j C Correct results were obtained for all controls lt Evidence Victim Define Alleles for Pick via mouse m Suspect Elim Y Evidence cw FIGURE 8 2 CODIS Disposition Form Window 91 ARMEDXPERT USER S MANUAL Fill in the top portion of the screen by filling in all necessary boxes If the information filled in at the top portion of the screen will be used more than once click on the duplicate button found at the bottom of the page This will open up another CODIS Disposition Form Window with the top portion of the screen fill in exactly like the original one CODIS Disposition Form for CODIS Administrator c E 2s CODIS Disposition Form for CODIS Administrator Po Po Category E EVIDENCE PROFILE for the FORENSIC INDEX This profile is from crime scene evidence is attributable to a putative perpetrator and is not from an item owned used by the suspect fhe source of the EVIDENCE PROFLE forthe FORENSICINDEKasbecnidentifed fhe source of the EVIDENCE PROFUE forthe FORENSICINDEX unknown Correct results were obtained for all controls FIGURE 8 3 T
101. he V arrow keys next to A box and another table using the V arrow keys next to the B box Once tables are selected click the Compare Tables button The two tables will be compared and a screen will appear as shown in Figure 6 9 below Any differences will be highlighted red Table Reference A 1 Table Reference B Compare Sources FIGURE 6 8 Sources Section Qo AtmedXpert D amp as QCCheds Match amp Compareson Interpretation Reporting E ga 5 ine hs U QU Ai DEMO 1 2 Component 4 Wt Mi stare gt 797 Sat Find Where Fed Incdodedin Find Foreign Find WhereSeferince Clear B DEMO I 2 Cowponent 4 v t Mix Stans Compare Reference Qelerence is Included th Reference to Reference Indudes Foreign Match Tables I Samples Sources DEMO_I_2 Component_4_Vict_Mix_Stains_Anatyst txt vs DEMO 1 2 Component 4 Vict Mix Stains Review bxt 0851179 21511 075820 CSF1PO 0351358 0135317 0165539 0251338 01954331 vwa 018551 Amel 055818 FGA Ladder idd 8 9 10 24 24 2 6 7 8 8 6 7 8 9 12 13 4 5 6 7 8 9 10 5 8 9 15 16 9 10 11 11 12 6 7 8 9 7 9 30 XY 7 89 17 18 Ladder ide 8 5 10 24 24 2 6 7 8 9 6 7 8 9 12 13 4 5 6 7 8 9 10 5 8 9 15 16 9 10 11 11 12 6 7 8 9 7 9 10 XY 7 8 9 17 18 ladder ide 8 9 10 24 24 2 6 7 8 9 6 7 8 9
102. ick on the highlighted cell and detailed information about that Locus displays Click on the detailed information to make it disappear For Example in Figure 7 4 above 21511 is highlighted By clicking on the highlighted cell the details for D21S11 for the selected sample appear as shown below in Figure 5 28 29 30 RFUs 916 1144 994 BPs 200 204 208 FIGURE T 5 Detailed information displayed for highlighted Locus 67 ARMEDXPERT USER S MANUAL Select number of Contributors Select the number of contributors using the Contributor selection box in the upper right corner The default number of contributor is 2 Click on the V arrow to change the number Contributor 2 M FIGURE 7 6 Number of contributors section Apply Reference s optional Next select any available references as permitted by your individual lab s mixture interpretation protocol Click on the Reference button under the Contributor selection box and the References Selection box will appear Click the Warrow in box 1 A drop down list of all your available samples will appear Click the one you would like to set as Reference 1 Once you select a reference the button next to the reference will change from Apply Reference to Remove Reference If you would like to remove a reference click on the Remove Reference button To set more than one reference proceed to Box 2 etc and repeat the steps above to apply reference References
103. iler patasource Commit D x 10891179 021511 pzsszo csr1Po 0351358 01353 041 Select items to commit to the datasource sp043 ae 50044 SD041 SD045 ded soose 1415 39 322 111 1112 1417 68 12 50047 S046 spots sboes n sposo soo 50051 SD051 50052 50052 soos m s0054 am soos X SD056 Ignore Case Also select tables 50057 058 SD059 SD060 SD061 5062 14 15 30 322 10 11 11 12 14 17 6 8 SD063 SD064 50065 50066 5067 11 14 28 9 10 1012 16 17 8 9 SNNAR 15 16 29 30 9 13 14 15 7 9 q 12 Table Individual Profiles Type Source Optional Commit 142 1592115 17 FIGURE 4 14 Datasource Commit Individual Profiles Window Click on the Individual Profiles tab Choose type of profile you are adding from the drop down menu next to the Type box The options are shown below in Figure 4 15 Source is optional to be filled in Click Commit button When finished with the window click the Done button to close the window Table Individual Profiles Type Staff Source QA SRM Unknown New profile type e 3 FIGURE 4 15 Drop down list 48 ARMEDXPERT USER S MANUAL To Load a sample table or sample that you committed from your database click on the Load Samples From Da
104. indow for the PHR Check 60 ARMEDXPERT USER S MANUAL Tab Four Match amp Comparison he Match amp Comparison tab is used to perform a match of a reference sample to other samples on your sample table or to compare one table to another table There are two sections Samples and Sources Q gt ArmedXpert i Views Data QC Checks Match amp Comparison Interpretation Reporting ow ben EY 7 G a A 1 RE a nal Set Find Where Find Included in Find Foreign Find Where Reference Clear B eference Compare Reference Reference is Included the Reference toReference Includes Foreign Match Tables Samples Sources FIGURE 6 1 March amp Comparison Tab The Samples section is used to compare a reference sample to other samples on a sample table To start matching you must first set a reference or references You can set any sample or samples as reference s Click on the Set Reference button The select pick via mouse window will appear as seen below in figure 6 2 The pick via mouse option will allow you to select the sample using your mouse Clicking the W arrow will provide a drop down list of all available samples from which you can select the appropriate sample To select more than one sample as references click on the Pick via mouse button and press the Ctrl button on your keyboard Then select samples with your mouse while keeping the Ctrl button pressed uy Views Data QC Checks Match amp Compar
105. inished click the Ok button Sort Sort FIGURE 4 19 Sort Section Sort Select the keys to sort by Sort will occur in the order that the keys appear in the second box Key Sort Order Sort As Key Sort Order Sort As Sort As A 0 Descending Normal E C 2 Descending Normal r Normal B 1 Descending Normal D 3 Descending Normal Normal 4 Descending Normal 1 gt gt Text As Numbers F 5 Descending Normal z G 5 Descending Normal H 7 Descending Normal 8 Descending Normal J 9 Descending Normal 3 lt K 10 Descending Normal L 11 Descending Normal lt lt M 12 Descending Normal 3ett Order N 13 Descendina Normal iN Descending w 4 IT Be Sort Used Range Sort Selected Range Ok Cancel FIGURE 4 20 sort Window 51 ARMEDXPERT USER S MANUAL Tab Three QC Checks he QC Checks tab is used to do quality control checks on your data There are four sections under the QC Checks They are Standard Checks CODIS QC QC Review and Clear The quality control settings for the checks are set up using the ArmedXpert Options Menu discussed in Chapter 2 views Data Oc Checks Match Comparison Interpretation Reporting ei Ke Y DN E Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Alleles Count A multi Review Checks Standard Checks C
106. ioiz i amp i5 R93 ii aya 1923 14 15 tamus FIGURE 3 4 View All Controls by Allele Q ps7 ArmedXpert SS views Data QC Checks Match amp Comparison Interpretation Reporting Alleles A M Headings 53 C Controls BasePairs Al Indicate clipped data Zoom 100 Organize e Samples _ RFUs C User li View Fit Il Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt cx E urs peoss poi 1 19 ess s 129 13 2024 25 2 14 12 82D Diss e pis m Xv 1 33 2811435 1 12 13 28 29 30 9 10 110 11 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18215 17 18 19 8 11 14 15 17 XY 9 12 22 22 2 24 25 _ 1213 28 29 30 9 10 10 11 12 15 16 6 9 38 11 12 9 12 13 19 20 24 25 13 14 14 2 182 15 17 18 19 8 11 14 15 17 XY 9 12 22 22 1 24 25 1213 29 30 10 1012 1516 693 812 1213 2024 15142 1719 8 11 1415 X 12 2224 13 910 1011 15 6 93 1 12 9 12 1925 14182 1518 11 1417 XY 912 22225 FIGURE 3 5 view All Samples by Allele 34 ARMEDXPERT USER S MANUAL Q p ArmedXpert CEU Views Data QC Checks Match amp Comparison Interpretation Reporting All Alleles Samples M Headings ai a J Controls Base Pairs lt All M Indicate clipped data 2 Zoom 100 Organize _ Samples _ RFUs _
107. ion Reporting CO All C Alleles Samples gt Headings i 2 4 o0 Controls BasePairs All M Indicate clipped data z Zoom 100 Organize 9 Samples RFUs 2 User g View Fit Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt c3 E 3 D851179 D21511 075820 CSFIPO D351358 THO1 D135317 D165539 D251338 D195433 TPOX 2 144 200 268272 322 326 124 171186 229 233 268 281 325 348 121139 171183 242 140 144 204 208 272 322 330 124 128 171 186 217 233 280 285 329 344 117123 179 187 230 242 144 200 268 272 322 326 124 171 186 229 233 268 281 325 348 12 139 17 183 242 FIGURE 3 8 view Samples by Base Pair 35 ARMEDXPERT USER S MANUAL Qo D ArmedXpert Views Data QCChecks Match amp Comparison Interpretation Reporting All Alleles Samples Headings z Controls Base Pairs F Indicate clipped data Zoom 100 Organize _ Samples RFUs _ User View Lo me Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt cx GUN CNET 0 01 E oore 755 879 921 1950 3472 1486 1806 1454 2288 839 758 961 904 1083 861 773 976 1115 1428 1597 1101 1887
108. ir corresponding alleles are imported into the page and ArmedXpert will automatically perform the calculations The combined likelihood ratio for each ethnicity is listed under the Likelihood column The prosecution s results are listed under the C1 column and the defense s results are listed under the C2 column Likelihood Ratio XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 c e XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 Locus Locus Profile Allele 1 a Allele 2 b Allele 3 c Allele 4 d Likelihood D851179 12 13 Ey Ea DEED PO g 12 13 1 12 13 0 1454 0 3393 0 23493409 0 1083 02222 0 10923025 01207 0 3251 0 19873764 D21511 28 29 30 PO g 28 29 30 P1 o 28 29 30 01658 0 1811 02321 0 335241 0 2151 0 1899 0 1788 0 34082244 0 069 0 2044 0 3301 0 36421225 D75820 9 310 Ee 30 PO g 9 10 1 6 9 10 0 1478 0 2906 0 19219456 0 1571 0 3238 0 23126481 0 0479 0 3062 0 12538681 CSF1PO 10 11 12 PO p 10 11 12 P1 9 10 11 12 0 2537 0 3005 03251 0 77316849 0 2714 0 2048 0 60248644 0 2536 0 2656 0 3923 0 83083225 0351358 15 16 32s 16 Ew Ea PO 9 15 16 P1 p 15 16 0 2463 0 2315 0 22829284 0 2905 03071 0 35712576 0 4258 0 2656 0 47803396 lt li X 1Px 0 EDT Set pner Reset Knowns Pick via mouse Denominator Reset Unknowns FIGURE 7 40 Likelihood Ratio Page
109. ison Interpretation amp 7 D tg UR Find Where Find Included in Find Foreign Find Where Referei Reference is Included the Reference toReference Includes Foreign gt Pick via mouse C DR FIGURE 6 2 set Reference Button 61 ARMEDXPERT USER S MANUAL Once you are finished selecting your reference s click on the sample table that you would like to match and compare This does not have to be the same sample table as the selected reference s Then click on one the four compare buttons Find Where Reference is Included Find Included in the Reference Find Foreign to Reference or Find Where References Includes Foreign To clear the match from the page click Clear Match button This also clears the reference from the page All matches are highlighted in a different color based on type of match There is a key on the bottom of the page In addition shown highlighted in blue on the bottom of the page is the selected reference unless the List match amp compare reference at top is selected as an option If selected the reference s is displayed at the top of the page instead of the bottom To the right of the samples are total columns These columns give a total number of each type of matches You can also do automatic sorts for these columns by enabling the correct default sort These options are selected by going to the Armed Xpert options menu as explained in Chapter 2 DEMO 2 Compo
110. ixture Information Section The second way is to manually select alleles in the middle section of the Mixture Interpretation Window Click on the Profile you would like to make a call for and then click on the boxes under the allele s you would like to select A check mark will appear in the boxes selected If you would like to make one of the alleles as an Any ot an Obligate right click on the checkmark box and a drop down list will appear You can also use this feature to remove or ignore an allele Select the appropriate option As shown in Figure 7 17 the word Any will appear left of the chosen allele if any is selected and a will appear left of the chosen allele if an obligate is selected 2 13 580 205 Victim M J J FIGURE 7 16 Calls made from Middle Section 12 13 1560 2345 vi 12 pd ignore Allele 2 1 Remove Allele FIGURE 7 17 Cals made from Middle Section 73 ARMEDXPERT USER S MANUAL l2 13 127 3 1580 2345 Mseg 2345 FIGURE 7 18 Examples of an 12 Any called and a 12 Obligate Called The final way is to click on the Popout calls button A window will appear as shown in Figure 7 19 below You can click on the box under the allele you would like to select for each Profile A check mark will show on all selected alleles By reclicking on a box that contains a checkmark it will be removed If you would like to call an Any or an Obligate right click on the box under th
111. l open In the print window you can change between orientations change sizes enter headers and print preview The More Option button will allow you to make additional changes to the way the page is printed Once you have made any changes click Print You can also set up saved page setups Make all necessary changes to the print setup Then click on the Save Setup button A Give the setup a name window will open Type in a user defined name Then click the Ok button When you want to use a saved set up select from the drop down list All saved changes will appear Then proceed to print or preview If you have an OSIRIS Plot file open the Plot tab will open instead for the Workbook tab Q EP Import 8 Print v Export gt 55 View b D FIGURE 2 36 Print Menu Print 5 Workbook Plot 4b Orientation Print Portrait Landscape Print Preview Print What Sheet Selection Workbook Scaling Zoom 100 Fittopages 1 Wide 1 lt Tal Options More Options Header Footer Sheet eader Footer ee TESTEN Left Header Select saved page setup Save as default Right Header d2 M3 y4 L Save Setup Armedxpert allows you to use special formatting flags help make your printing process easier CLT DeleteSetup Flag Meaning Example Product version Armedxpert v3 0 7 773 Data source file name if applicable MyDNAData
112. leles Count x multi Review Checks Standard Checks E CODIS QC QC Review Clear C Program Files NicheVision ee Insen Peen Test Genotypes Table txt D195433 04 01 13 43 30 30 1011 10 12 3415 553 aot 10 x olo ola oia Y ani mm is un partes ongorsI miu rr Dus fashi 10 11 13 24 29 30 8 9 10 12 9 10 11 12 16 17 8 9 9 3 10 11 12 11 12 17 18 20 25 26 12 14 2 15 1617 18 19 8 34 18 XY honaxiupso sz sung i 7 rss salon a Fame mule ae ae ae x Y mms ms iu nu aw ssa zu mae usu nose x 10 11 13 14 29 30 8 9 10 12 9 10 11 12 16 17 8 9 9 3 11 12 11 12 17 18 20 26 12 14 2 15 16 17 18 19 8 8 14 18 XY 9 30 5 9 10 1219 10 11 12 16 zjessponspus wem ETE COE T r 3 in onu 8 9 9 3 10 11 12 11 12 17 1820 12 14 2 15 16 18 19 8 8 14 18 Deus 9 30 8 9 10 12 9 101 1617 5 9 93 14 18 Mi on T 5 EN e EEG De T nae os BRA Ar items was na diha for si check FIGURE 5 14 PHR Result Example The Check Multi command checks samples with identical names and confirms that they are identical at each Locus Any Locus that is not identical is highlighted pink For example in Figure 5 15 below at D3S1358 alleles 15 16 are showing for XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 F01 Sample but alleles 15 16 17 are showing for the identical sample right below i
113. lls Window You can click on the V arrow next to Locus Box for a drop down list of all loci and then individually select the locus Finally you can click the lt or gt arrows to move either forward or backward to a different locus Setup 0156 1 3892 338361 15 2F1 Shit Vict O6 0b 20 D01 Operations Contributor E References Locus D85117313 12 13 P Avg 0 71 PH 0 40 2 Suspect n a P Avg 0 29 J WnoreAlleles ur 00 3 Alleles 12 13 Below mPH x 3 RFUs 1580 2345 Lock locus BPs 139 55 144 26 Popout View call View call report cah a gt Add Comment 12 13 100 15 1580 2345 Apply i J Globally Apply Stutter Add Profile 0 08 Mixture Information FIGURE 7 21 Moving to a different Locus 75 ARMEDXPERT USER S MANUAL Additional Features There are four additional features to help you make calls with the Mixture Interpretation Window The first is Highest to Lowest option By clicking on the box next to Highest to Lowest the Loci will be arranged for selection from the locus with the most alleles to the ones with the least Note Changes made to the alleles while making your interpretation like deleting an allele or adding stutter can change the order of the Loci when using the Highest to Lowest option Mixture Interpretation DDA Interpretation Setup 7 Highest to Lowest Locus D
114. ls LOMUDULOT Operations 7 1 15 2F 1 Shirt Vict 06 06 20 D01 Operations Mixture Frequency i 5 Electropherogram Hinhest tn CEA OPS Open OSIRIS Plot File FIGURE 7 32 Operations Button Virtual Electropherogram V Use single plot Use multiple plots Mixture Interpretation Freque gt RMP Method Modified RMP Modified RMP Apply Restricted Globally _Fiestricted AMP nml Contributors 2 gt Open Frequency Report Clear FIGURE 7 33 Mixture Interpretation Frequency Window NOTE Do not close out of the Mixture Interpretation unless you have exported saved or printed the reports you require to document your interpretation If not once you close out of the Mixture Interpretation tool all information will be lost 80 ARMEDXPERT USER S MANUAL Frequency Calculations The Frequency Calculation section allows you to perform different frequency calculations ArmedXpert includes frequency calculations for Single Source Likelihood Ratio Probability of Inclusion and Mixture It can perform statistics on both single source samples and a two person mixture It can calculate both probability of inclusion and probability of exclusion for single source and mixture samples As well as calculate likelihood ratios for mixture samples e gt m ZN 3 x amp Y Single Likelihood Probability Mixture Source Ratio of Inclusion
115. m 100 Organize Samples J RFUs _ User View Fit Zoom Show Hide Windows esta asst orum sei Sco pasase muor prasot7 pressas pase mur 8551 5581 EZALATT ATILLA 1 Ladder Identifiler 06 06 20 A03 3 9 10 25 242 67 5 67 55 42 13 45 8 9 10 85 151617 191 12 1 1213 67 7 9 10 XY 7 8 Ladder Identifiler 06 06 20 F03 7 8 5 XX0153 99Xx9999Q1 15 12F1 Shirt Vict 06 06 20 02 X Y 9 12 FIGURE 3 18 Headings Shown Qo gt ArmedXpert views Data QC Checks Match amp Compare porting All Alleles Samples R Headings E Controls e 3 Base Pairs All 3 V Indicate clipped data Zoom 100 prganize Samples _ RFUs _ User View Fit Zoom Show Hide indows DEMO 2 Component 4 Vict Mix Stains Analyst txt Ladder Identifiler 06 06 20 A01 Ladder Identifiler 06 06 20 A03 Ladder Identifiler 06 06 20 F03 ossius onis ovszo cerro Toastase mon sssz paessse bosse sass wa reox meussti FA 3 9 10 FALSE 24 6 7 8 6 7 8 9 12 13 4 5 8 9 10 5 8 9 15 1617 9 10 11 12 11 12 13 6 7 7 9 19 X Y 17 18 19 2 CAEN ELIE CRAM RAED iei CEN CS EA sem NTT nus ez AON EAT 7 8 9 17 18 19 2 78 9 17 18 19 2 xx0153 99xx999901 15 12F1 Shirt Viet 06 06 20 H02 15 24 10 10 11 is ess 11 12 912 1925 14182 151 11 1417 XY 9
116. move a check that is shown or add a check to an empty box ARMEDXPERT USER S MANUAL The second main section is Data This section allows you to assign allele exclusions to what is imported into ArmedXpert from your sample data Any artifact that is listed and has a check mark will not be imported into ArmedXpert There are a few default items already assigned in Armed Xpett You can add new exclusions by clicking the Add Exclusion button You can turn off an exclusion listed by clicking on the box next to each title and removing the check mark Allele exclusions can be deleted from the list by clicking on the exclusion so it is highlighted then clicking on the Delete Exclusion button 5 vy X ArmedXpert Options ca General Sample source import options Allele exclusions Interpretation Exclusion Title Artifact Pull up pu Minus ma V Spike sp Stutter st Quality Control Reporting Admin Console Add Exclusion Delete Exclusion Ok Cancel FIGURE 2 6 Data options ARMEDXPERT USER S MANUAL The third main section is Interpretation This section has four subsections Mixture Interpretation Quality Control Frequencies and Frequency Options The first subsection Mixture Interpretation is made up of two sections The top section Mixture interpretation settings allows you set the default mixture interpretation settings by adding default values into the box right of each typ
117. move pnorut snow coos Remon Tid CHANGE SOURCE ID to YES Reason ct results were obtained for all controls Evidence Victim 3X72 2 995678 Q1 2 F2 Vag Swab 06 07 14 002 Dossssoezskis Vetmet 007 1449 o o 0851179 11 12 13 14 2344 2078 1439 1756 1151 2030 021511 29 30 30 2 32 22225 2268 930 838 D75820 980 905 2409 569 CSF1PO 10 11 12 13 1177 2505 923 1047 0351358 16 1969 1434 1713 THO1 1194 2697 1115 1382 1140 D135317 632 751 854 1376 D165539 1218 1736 2292 1034 D251338 18 20 22 23 25 905 776 1456 1004 620 0195431 14 14 2 15 15 2 3226 1520 751 937 VWA 2365 1805 1211 912 TPOX 2467 4483 018551 524 773 2009 494 Amel 4261 1602 055818 3348 633 1186 FGA 20 21 22 23 321 450 1336 513 Suspect XX95 9910 5678 K9 Susp 1 Ref 06 07 14 F07 0851179 1 13 45474746 021511 30 30 2 3725 3575 075820 9 10 2943 2891 CSF1PO 10 12 3043 2797 D351358 15 17 3192 3051 THOL 7 93 4031 4198 0135317 3213 2604 0165539 5592 0165539 0251338 20 25 2889 2772 0251338 VWA 17 18 3018 2803 D18551 16 17 2608 2186 13 18 1761 1372 Amel v 2362339 Y 5 5 33620431 D55818 u 67 7 FGA 2003 1744 Analyst nitials d te FIGURE 8 6 CODIS Disposition Form filled out 93 ARMEDXPERT USER S MANUAL Total Data The Total Data report is a report that gives you
118. mple s Click the gt button to move the selected table or sample to the left right side LI a A s _ Select a table c3 e Samples amp sources Selected samples amp sources DilutionS eries xls 1 5 Dilution 1 5 Dilution 1 0 dilution 25 Dilution 25 Dilution 1 0 dilution gt Ignore Case Also select tables Ok Cancel FIGURE 8 16 Select a table Window Once all tables or samples have been selected and moved to the right side click on the Ok button 100 ARMEDXPERT USER S MANUAL The PHR Report will open back up Your chosen selections will appear Q PHR Report c3 e Description Table 1 5 Dilution 1 5 Dilution 1 0 dilution 1 0 dilution 25 Dilution 25 Dilution Remove Double click an item to change Add a table selected its description Dk Langel FIGURE 8 17 PHR Report Window with selections If you would like to change any of the table s description names before you run the report click on the table from the list shown An Enter a description Window will open Type in the new description and click on the Ok button PHR Report Description 1 5 Dilution 1 0 dilution 25 Dilution Table 1 5 Dilution 1 0 dilution 25 Dilution Enter a description X Ok Came Remove Add a table absint Double click an item
119. mum number of included to 1 as shown below In the Profile Type box add a checkmark to all the profile types you would like the tables to be compared against To disable this reporting option click on the box with the check mark and it will be removed Note If this option is not checked you can manually check a staff profile against any sample table in ArmedXpert The sample comparison reporting directions can be found in Chapter 8 ArmedX pert Options TI General Reporting Options CODIS Data Interpretation Reporting options Quality Control Compare tables to profile types selected below upon opening Reporting Values are inclusive Use 1 Admin Console to match any amount Profile Type Staff SRM Unknown Ok Conrad FIGURE 2 19 Reporting Options window 20 ARMEDXPERT USER S MANUAL The second subsection CODIS allows you to add defaults to the CMF Report in ArmedXpert All data filled in here will be displayed with you open the CMF report X tal ArmedX pert Options c3 5 General Reporting Options CODIS Data Common Messaging Format CMF Default CMF Attributes Interpretation Quality Control Source Lab Destination Lab User CODIS ID Admin Console Imaging System Organization Imaging System FIGURE 2 20 CODIS Option Window 21 ARMEDXPERT USER S MANUAL The final section is Admin Console This section will only be av
120. n Interpretation Reporting Alleles 4 Samples M Headi 2 Mama XJ _ Controls Base Pairs All Indicate clipped data Zoom 100 Organize 4 Samples 5 View Ft Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt cx s ETONETONNUITRENCI ONE C OGOMUMENE NEN ONE Diss ips eny emere meae ams 5 215s 1827 2500 2265 aos 7 e 57 1 246 402 332 328 1 6 LA 9 3915 4443 3448 3618 2698 2497 2333 2426 2489 2309 2299 2202 2328 2316 2449 2410 2734 2721 7403 6117 2224 2344 2584 2804 4063 2468 1997 2155 1827 2560 2265 2316 2427 2384 2157 2968 3302 2833 2655 FIGURE 3 11 view Samples by RFUs 36 ARMEDXPERT USER S MANUAL Fit The Fit section allows the user to determine the width of the columns Samples change the column width to fit all sample data only All changes the column width to show all the data for both the samples and the controls User will keep the columns the user defined width when changing the views The column width remains the same no matter what is being viewed Click on the circle to the left of the desired choice Samples I User Fit FIGURE 3 12 Fit Section Q gt ArmedXpert hs Views Data Qc checks Match amp Comparison Interpretation Reporting All Alleles C Samples 3 E Headi
121. nent 4 Vict Mix Stains Analyst txt jD7S820 CSFIPO D351358 THO1 D13S317 D165539 D251338 D195433 vWA TPOX D18S51 AmelDSSSIS 2 1Referd 9 10 10 11 15 693 11 12 9 12 1925 14182 1518 11 1417 XY 9 12 XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 H02 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 FIGURE 6 3 Reference displayed at the top of the page option Note There ate two additional quick ways to choose your reference You can also click on the appropriate sample that you want to set as a reference and press the F1 key This will automatically highlight the sample you are on and start the Find Where Reference is Included comparison You can also just select with your mouse the sample s that you would like to set as the reference then click one of the compare buttons These two only work if the reference is on the same sample table as the one that you want to match and compare to Find Where Reference is Included is used to find loci that include the reference All loci that contain an exact match to the reference are highlighted orange All loci that contain the reference and additional allele s are highlighted yellow DEMO 2 Component 4 Vict Mix Stains Analyst txt c s ES D75820 csrimo 0381358 THo1 0135317 0168539 251358 0198033 vwa TPOX 018951 Wmel DSSBIB FGA Ladder Identifiler 06 06 20 A01 4 5
122. ngs Controls C Base Pairs All 3 M Indicate clipped data Zoom 100 Organize C Samples J RFUs _ User View FP Zoom Show Hide Windows DEMO_I_2 Component_4_Vict_Mix_Stains_Analyst txt 0851179 075820 8 9 10 11 12 13 14 15 16 17 18 19 FALSE 24 25 26 27 28 28 2 29 29 2 30 30 2 31 31 2 32 32 2 33 33 2 34 34 2 35 35 2 36 37 38 6 7 8 9 10 11 12 8 9 10 11 12 13 14 15 16 17 18 19 24 24 2 25 26 27 28 28 2 29 29 2 30 30 2 31 31 2 32 32 2 33 33 2 34 34 2 35 35 2 36 37 38 6 7 8 9 10 11 12 8 9 10 11 12 13 14 15 16 17 18 19 24 24 2 25 26 27 28 28 2 29 29 2 30 30 2 31 31 2 32 32 2 33 33 2 34 34 2 35 35 2 36 37 38 6 7 8 9 10 11 12 28 1 12 13 28 29 30 Al 12 13 28 29 30 9 10 13 14 28 31 1 11 12 1 12 13 28 29 30 12 13 28 29 30 9 10 A E 9 12 FIGURE 3 13 Column widths ate widen to show All Q E ArmedX pert views Data QC Checks Match amp Comparison Interpretation Reporting e All Alleles Samples 7 3 Headin SOS om a a Controls _ Base Pairs All F Indicate clipped data Zoom 100 Organize _ Samples _ RFUs I User View Fit Zoom Show Hide Windows DEMO 2 Component 4 Vict Mix Stains Analyst txt EET
123. oad the table Note The table has to be committed to the database to appear in this window If you have not committed the table you would like to use go to the instruction on page 46 of this manual for instructions on how to Commit Samples to Data Source Datasource Load 0 X Samples amp sources Selected samples amp sources LADDERSANDCONTROLSCHECK_TABLE 22 3 8 Ignore Case Also select tables Search J Search Tables Search Profiles Delete Load FIGURE 2 18 New Table Selection Window 19 ARMEDXPERT USER S MANUAL The fifth section is Reporting This section has two subsections Reporting Options and Templates The first subsection Reporting Option allows you to set ArmedXpert to automatically check all samples against the loaded selected profiles as soon as you import the table into ArmedXpert When checked ArmedXpert will generate a report when a table is imported into ArmedXpert It lists all the samples included on the imported table and indicate which ones are at equal to or above the minimum number set for exact and included matches compared to the selected profile types To enable automatic table and staff profile comparison check the box to the left of Compare table to profile types selected below upon opening Then enter the minimum number of exact matches you would like to show on the report and the minimum number of included matches To show all included set the mini
124. ons change based on the values set You can add a reference contributor to either Numerator prosecution or Denominator defense Choose either Numerator or Denominator by clicking on the circle next to the wanted choice The circle will be filled in as shown below to indicate the chosen one Then either click on the Pick via mouse button and select your reference or click on the V arrow and select the reference from the drop down list of samples The reference s alleles will appear in green at each Locus as shown in figure 7 42 on the next page Numerator ResetKnowns 1Px 2Px 1 Set Pick via mouse A157 33 663333 1 11 Blood Vict 06 06 20 G02 5 Denominator Reset Unknowns _ nen rr FIGURE 7 42 Bottom of Screen of the Likelihood Ratio Page The Reset Knowns button resets all unknowns red back to known green for either the Numerator or Denominator depending on which is chosen The Reset Unknowns resets all knowns green to unknown red for either the Numerator or Denominator depending on which is chosen 84 ARMEDXPERT USER S MANUAL Double click on any allele to change from Green Known to Red unknown or vice versa All calculations will automatically recalculate with all changes done to the page At the bottom of the page are the calculations by ethnicity for the entire profile The calculations are shown in both exponential and alphanumeric form An exam
125. op Portion of the CODIS Disposition Form Window Four different samples can be added to the report Evidence Victim Suspect and Elim Suspect2 this can be changed to either type by clicking the arrow next to Elim and choosing from the drop down list add a type first click on the circle next to the type you would like to add This will fill in the circle The select pick via mouse window is next to the type selections You can use your mouse to select the sample you wish to choose or click on the VW arrow and select the one you would like to add from the drop down list This will fill in the information from the sample table Continue with the next type until you finished adding all the necessary types Note The different types of samples fill in the report in the order you add them So if you want the Evidence to appear first make sure you add the evidence first to the report and so on il lt gt par a Evidence Victim Define p Alleles for Pick via mouse Duplicate Suspect Elim Evidence I FIGURE 8 4 Bottom of the CODIS Disposition Form Window There is also a define alleles for evidence option This adds a column of alleles next to the evidence that you want to send to codis So add your evidence first and then click on the circle next to Define Alleles for Evidence Choose the sample you want to add from the Mixture Interpretation Call View Report you created when using
126. ord Role DefaultAdmin M Review Settings Ok Cancel FIGURE 2 23 Edit User window To delete a user click on the user name you would like to delete Then click on the Delete User command The User name will be deleted 23 ARMEDXPERT USER S MANUAL The second tab is Database When you upgrade ArmedXpett it is may be necessary to run the SQL updater This will update your SQL database to your new upgraded ArmedXpert version Click on Run SQL updater command This will update SQL Arme dX pert Administration Users Database Run SQL Updater Database User Name First Name Last Name DateCreated admin 10 22 2010 1 35 PM FIGURE 2 24 Database Window 24 ARMEDXPERT USER S MANUAL Import The Import command allows you to load a Tab delimited table files such as GMIDIdntifilier GMIDPowerplex 16 Profiler Plus and others It also allows you to load other table types such as previously exported page data Osiris Analysis Report and CODIS Index file Finally you can load other files such as previously exported frequency analysis calculations previously exported mixture interpretations and OSIRIS Plot files Click on Import and the list below will appear Select the appropriate type of file you wish to import Once in Armed Xpert you can export the sample tables as Page Data You can then import it back into Armed Xpert using the Page Data option The method is designed to give use
127. played at amelogenin It will highlight in pink a Sample s Locus that contains a single X Q z ArmedXpert views Data QCChecks Match amp Comparison Interpretation Reporting 4 P X H E 1 amp WINZEN Check Check Allele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Aleles Count X multi Review Checks i Standard Checks le CODIS QC Review Clear DEMO 2 Component 4 Vict Mix Stains Analyst txt e D8 1179 21511 D7S820 CSFIPO D351358 THO1 D135317 D165539 D251338 D199433 vwa TPOX 18551 Amel DSSB8 FGA 12 13 28 29 30 9 10 10 14 12 15 16 6 9 3 8 11 12 9 12 13 19 20 24 25 13 14 14 2 182 15 17 18 19 8 1114 15 17 XY 9 12 22 22 2 24 25 J1 12 13 28 29 30 9 10 10 11 12 15 16 6 93 8 11 12 9 12 1319 20 24 25 13 14 14 2 18 2 15 17 18 19 8 11 14 15 17 XY 9 12 22 22 2 24 25 12 13 28 29 30 9 10 10 14 12 15 16 693 8 11 12 9 12 13 19 20 24 25 13 14 14 2 18215 17 18 19 8 11 114 15 17 XY 9 12 122 22 2 24 25 11213 29 30 10 1012 15 16 693 812 1213 12024 13142 1719 jauj 1415 X 12 1224 3 si 15 693 112 912 1925 14182 1518 aa 1417 xv 912 2225 FIGURE 5 12 Check X Result Example The OSD off scale data command evaluates the RFU s for off scale values The OSDs settings are set up under the Quality Control S
128. ple of the bottom of a Likelihood report can be seen in Figure 7 41 on the previous page Likelihood Ratio XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 c XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 Locus Locus Profile Allele 1 a Allele 2 b Allele 3 c Allele 4 d D851179 2 15 a2 f 021511 28 2 so D75820 CHEN 0 1478 0 2906 0 1571 0 3238 0 0479 0 3062 CSF1PO 10 11 12 n 2537 n 3200S n 3251 aa lt 1 gt CON t 1Px 0 2P 1 245 e Reset Knowns_ XX0167 99 X9999K1 11 Blood Vict 06 06 20 802 9 Denominator Reset Unknowns FIGURE 7 43 Likelihood Ratio Page with Reference Applied 85 ARMEDXPERT USER S MANUAL The third type of frequency calculations is a Probability of Inclusion calculation It calculates both probability of inclusion PI and probability of exclusion PE for single source and mixture samples To obtain the Probability of Inclusion Calculation page click on the Probability of Inclusion button The Select Pick via mouse window will appear Select the sample you wish to perform the calculations on with your mouse or click on the V arrow and select from the drop down list FIGURE 7 44 Select Pick Via Mouse Window The Probability of Inclusion Calculation page will appear All Loci and their corresponding alleles are imported into the page and ArmedXpert will automatically perform the calculations The freq
129. race Then proceed by clicking on the tab key to each next column Type in the locus the Allele and the Frequency amount in the proper columns Clicking on the tab key will take you to the next line Continue manually filling in all the data Note Make sure you enter in all the data correctly The Race and Locus cannot be edited later So if either are incorrect they cannot be changed once you commit the frequencies to ArmedXpert To paste in frequencies first open the data into excel It should be in the same order Race Locus Allele and Frequency Highlight all the data you want to paste into ArmedXpert Click the Copy button in Excel Go back to ArmedXpert and click on the Paste button All the data will show up in the Text Import Window Click the OK button All the data will show up in the Add Frequency window To import frequencies from a file click on the Import from file button Select the appropriate txt or csv file from the Open window The Text Import window will open Click the Ok button All the frequency data will appear in the Add Frequencies window Once all frequencies have been added click on the Commit button Add Frequencies Source Description Optional Comrnit Paste Import from file Allele Frequency FIGURE 2 13 Add frequency window Click on the Review Frequencies button to review Frequencies You can also make changes to the Frequencies from the revi
130. ributor 2 allele mixture of types AA amp AB 2 2 combination s 7 7 Ref 1 1 81 Ref 1 1 2 13 phr 0 63 2 3 phr p 0 9 2 p 0 03 3 p 0 19 1 00 O For a 2 contributor 2 allele mixture of types AB amp AB 1 1 combination s ME 67 Ref 1 12 13 pl ar 0 6 7 FIGURE 7 14 Three different ways to make calls The first way is to right click the combination you would like to call The Send to Profile 1 and Send to Profile 2 buttons will appear If you are doing a 3 contributor mixture interpretation a Send to Profile 3 button will appear also Right click on the genotype combination in the combination row you would like to call and select the particular profile by clicking the appropriate Send to Profile 1 2 or 3 button Then repeat for the next profile until all profiles have been chosen Once selected the information will be appear in blue as show in the example below in Figure 7 15 Genotype 12 13 1s applied to Profile 1 Victim and Genotype 13 13 15 in process of being applied to Profile 2 Suspect 72 ARMEDXPERT USER S MANUAL Mixture Information f a 2 contributor 2 allele mixture of types AA amp AB 2 2 combination s 12 13 phr 0 63 p 0 97 Ref 1 12 p 0 03 12 13 phr 1 00 p 0 81 victim Ref 1 41 Sir For a 2 contributor 2 allele mixture of types AB amp AB 12 13 phr 0 67 Ref 1 12 13 phr 0 67 FIGURE T 1 5 Calls made from M
131. rs the ability to return to the original files or open working copies stored in the Page Data format Tab delimited table files GMIDIdentifiler Ww Export gt GMIDPowerplex16 GMIDProfilerCOfiler View gt 5 Profiler Plus GMIDSizeStd Print b GMID filer GTProfilerCOfiler 066600000 Other Other table types Page Data Import samples that were previously exported as page data Osiris Analysis Report E Import report generated by the Open US Source Indep Review Interp System CODIS Index 1 Import a CODIS Index File Other Files Frequency Analysis Load a previously exported frequency analysis sheet to resume interpretation Mixture Interpretation Resume an exported mixture interpretation OSIRIS Plot File Load a plot File generated by OSIRIS FIGURE 2 25 Import Menu 25 ARMEDXPERT USER S MANUAL Once you select the type of data you are importing the open window appears shown below in Figure 2 26 Select from the list the file you wish to import Then click the Open button Note If the file is located in another folder change the folder by clicking on the arrow next to the Look in box The table files will then open in Armed Xpert as shown in Figure 2 27 below The file name will appear on the upper left hand corner of the page E DEMO_I_2 Component_4_Vict_Mix_Stains_Analyst txt DEMO I 2 Component 4 Vick Mix
132. s on the sample table Click on the entire sample table or the specific sample you wish to combine and then the arrow gt button The sample table or sample then will move to the Selected samples amp sources box on the right Repeat for the next sample table or sample The gt gt button will move all samples at once You can also use the Selection Filter box to enter in a specific name and all samples containing that name will be highlighted This is helpful in finding certain samples in a large sample table Click the gt button and all samples highlighted will move to the Selected samples amp sources box The Ignore Case next to the selection filter box should be checked if you would like the selection filter choice to include all samples containing the filter regardless of upper or lower case The Also select tables option should be checked if you would like the selection filter to choose tables as well as samples that contain the filter 44 ARMEDXPERT USER S MANUAL If you wish to remove a sample table or sample from the Selected samples amp sources box click on the sample table or sample and then click on the lt button By default the names of all selected samples will automatically fill in as the title of the combined report You can keep the default title or you can type in a new name for the combined report that is being created Click on the Combine button to create the combined report Combine Sour
133. se Pairs 142 61 155 75 Base Pairs 234 84 236 89 24 FIGURE 4 6 Edit Sample Window 43 ARMEDXPERT USER S MANUAL To combine sample table s or sample s into one new combined sample table click on the Combine Source button This will open the Combine Sources window Note Each of the sample tables must be open to use this feature Combine Sources c3 E Select items to combine Samples amp sources Selected samples amp sources DEMO_ _2 Component_4_Vict_Mix_Stains_Analpst tet A DEMO 2 Component 4 Vict Mix Stains Review t t Ladder Identifiler 06 06 20 F03 Ladder Identifiler 06 06 20 403 Ladder Identifiler 06 06 20 401 0155 1 99 lt 999901 15 2F1 Shirt Vict 06 06 20 veu 220156 3 9999990 1 15 2F3 Shirt Vict 06 06 20 E 2040159 1 99499990 1 15 8F1 Shirt Vict 06 06 20 F xc 0151 1 QAO03F1 06 06 20 H01 0162 1 Reag BlankF1 06 06 20 B02 0162 2 Reag BlankF2 06 06 20 C02 240164 33 24338301 2 LFS Vict 06 06 20 D02 280165 gADO4 QA 06 06 20 E02 lt lt gt lt 0166 Reag Blank 06 06 20 F02 m XXD157 9949999K1 11 Blood Vict 06 06 20 G02 20168 99XX9999K2 Blood Susp 06 06 20 H02 260169 G4026 QA 06 06 20 B03 20170 Reag Blank 06 06 20 CO3 v 3 J Ignore Case Also select tables Title Combine FIGURE 4 7 Combine Sources Window The Sample amp sources box displays all open sample tables By clicking on the next to the Sample Table name the list will expand to show all available sample
134. t z ArmedXpert DEMO 2 Component 4 Vict Mix Stains A Views Data QC Checks Match amp Comparison Interpretation Reporting ls A a SHOVES x Check Check Allele Stutter RFUs CODIS Allele Check ae PHR Check Begin Clear Controls Ordering Alleles Count x multi Review Checks Standard Checks CODIS QC QC Review Clear passat UN TRE D13531 156 3999 2F3 06 20 E 01 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 1 5062 E01 2 28 29 30 9 10 10 1 1 EISE 8 11 1 12 13 178 29 30 9 10 sag 15 16 9 3 8 11 1 1 2 EDERES 29 01 8 10 1 15 16 17 6 9 3 8 11 1 28 29 30 9 10 10 11 e 9 3 8 11 1 28 29 30 9 10 10 11 12 15 16 6 9 3 8 11 1 1213 29 30 10 10 10 12 15 16 6 93 8 12 12 13 29 30 10 10 10 12 15 16 6 9 3 8 12 13 13 28 28 9 10 10 11 15 15 6 9 3 11 12 FIGURE 5 15 Check Multi Result Example 58 ARMEDXPERT USER S MANUAL QC Review The QC Review section only has one command It is Begin Review This command gives you detailed information of all the highlighted results shown during each of the checks It explains why the locus was highlighted an Begin Review Qc Review FIGURE 5 16 QC Review Section After performing a check click on the Begin Review command This must be done before you click on the Clear Checks command To move to the next or previous highlighted Sample s Locus ther
135. t Tl or l 7 gt PEJ 7 Upper case letters Lower case letters All letters Digits and letters Digits Hexadecimal digits Punctuation Space and tab Blank characters Control characters Printed characters Printed characters and spaces Digits letters and underscore Lookahead assertion Negative lookahead Lookbehind assertion Negative lookbehind Once only Subexpression Condition if then Condition if then else Comment Items marked should work in most Note regular expression implementations USER S MANUAL A Za z0 9 d41 2 Ad 1 2 Vd 4 s 251 palagif pna 2 1 9141 1 4 14 1 0 9 11 50 amp A Fa f0 9 3 A Fa f0 9 43 2 Xd 2 a z 2 A 2 8 15 wrib a zA Z 4 X a 2A 2 12 6 sU A A Letters numbers and hyphens Date e g 21 3 2006 jpg aif or png image Any number from 1 to 50 inclusive Valid hexadecimal colour code 8 to 15 character string with at least one upper case letter one lower case letter and one digit useful for passwords Email addresses HTML Tags Please use with caution and test tharaughly before use Note These patterns are intended for reference purposes and have not been extensively tested Quantifiers or mare nii 0 or more ungreedy 1 or more 7 1 or more ungreedy 1 Ti or 1 ungreedy 3 Exactly 3 4 13 3 or more 3 5 3 4or5 3 5
136. ta Source button The Datasource Load window will appear All committed tables and individual samples will show up in the left hand side box The individual profiles are shown highlighted green below in Figure 4 16 and the table is highlighted tan Click on the next to the Sample Table if you wish to select a specific sample from the sample table Click on the sample or the whole sample table you wish to commit click the gt button and your selection will be move to the Selected samples amp soutces box on the right Once finished with your selection click the Load button The Data will load automatically on its own page Datasource Load c3 Samples amp sources Selected samples amp sources LADDERSANDCONTROLSCHECK_TABLE LADDERSA amp ANDCONTROLSCHECK TABLE w Oo ce fa wo c ce 4 NA en ue SD053 lt lt Ignore Case Also select tables Search Search Tables Search Profiles Delete Load FIGURE 4 16 Datasource Load Window If you would like to delete a sample table or a specific sample from a committed Datasource you do it from this window Move the table or sample you want to delete to the right side of the window as described above To have the individual profiles to show like the Staff Profile click on the box next to Search Profiles In the example shown in Figure 4 17 below an individual staff sample was deleted from the staff profile On
137. table page If you prefer for the selected sample to not be highlighted uncheck the box next to Highlight in Mixture Interpretation Section as shown below in figure 7 4 Qo M ArmedXpert 5 5 pc Checks Match amp Comparison Interpretation Reporting amp 5 8 Y Single Likelihood Probability Mixture Source Ratio of Inclusion Begin Mixtu Interpretation Mixture Interpretation Frequency Calculations DEMO 2 Component 4 Vict Mix Stains Analyst txt E 2 Mixture Interpretation DDA Interpretation E Setup xc Pick via mouse I ex0156 1 99 lt 9999011 15 2F1 Shirt Vict 06 06 20 D01 Contributor 200 5 r Highest to A gm Hires Profile 03 Victim 29 30 RAW Profile 04 Suspect n a P Avg y mPH SO HI i 5 1 Poo t RFUs 318 1144 394 P Look toous pif ov BPs 200 204 208 Zi ET Popout Vier callrepot cals Add Comment 7 3 94 100 Appl lAddPofle O wm m 0 09 Iri rf Mixture Information 2 5 combinations have PHr 2 0 MPh gt IRSE i LLCCECELCCZCELLELCCOC CLY TPTIS2S 72 SSC18 28 CEILIGCLELUEIt1 i For a 2 contributor 3 allele mixture of types AA amp BC 3 3 combination s T j 29 30 phr 0 87 p 0 70 28 p 0 30 28 29 phr 0 80 p 0 67 30 p 0 33 28 30 phr 0 92 p 0 63 29 p 0 37 FIGURE 7 4 Example of screen once sample is selected Note You can cl
138. ted yellow 5 ArmedXpert DEMO 2 Component 4 Vict Mix Stains Analyst txt Views Data Checks Match amp Comparison Interpretation Reparting amp M Ke CY D x Check Check Allele Stutter RFUs CODIS Allele Check a PHR Check Begin Clear Controls Ordering Alleles Count x multi Review Checks Standard Checks CODIS QC QC Review Clear Ladder Identifiler 06 06 20 A01 pre Ee Oe ee 7 9 10 SEE 9 17 38 19 20 Ladder Identifiler 06 06 20 A03 ss pemilsns eras nes sem sas T 1816 17 snm nois ez zen xv zas 1290 20 Ladder Identifiler 06 06 20 F03 310 2602187816789 1218 PES 83910 3089 118 07 910 H E 111213 67 17 9 19 E R9 1 181920 XX0153 99XX9999Q1 15 12F1 Shirt Vict 06 06 20 02 Lui roo sso sos ss esaf uo fini ur vl ota as XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 13 14 inis inisis 8 11 XY 9 12 22222 2425 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 E01 8 3 uec SEEN XX0159 1 99XX9999Q1 15 8F1 Shirt Vict 06 06 20 01 46 ta tal Ge ae eo EDEDE E 2 15 17 18 19 8 11 14 15 17 x Y 9 12 22 21 XX0161 1QA003F1 06 06 20 H01 151 28311 1 12 2930 i amp is amp e amp 9 iis ma nu mi jaul i kyni 2225 XX0162 1Reag BlankF1 06 06 20 802 BERE ERES ERE T RET aia E XX0162 2Reag BlankF2 06 06 20 CO2 SS XX0164 99XX9999Q1 2 LFS Vict 06 06 20 D02 1 14 15 17 X Y 9 12 22 22
139. tions General Options Ladders and Controls Ladders and controls options Control qualifiers Qualifier Description Expression All Controls Pos ConlNeg ConlReag Blank LadderlQ AQ Positive Control Pos Con POSCON Negative Control Nea Con HEGCON Reagant Blank Reag Blank II REAGBLANK Ladder Ladder Quality Assurance Sample QAH Reporting Admin Console s Select the table to use when checking controls LADDERSANDCONTROLSCHECK TABLE Select Table i Ok Cancel FIGURE 2 1 Ladder and Controls Subsection On the next page is a list of regular expressions These should only be changed if necessary This is an advanced user form and should only be changed by those who understand regular expressions 17 ARMEDXPERT Jmm regularexpressions VA 5 Lea b B Va Character Classes Ac 5 MS Ad AD Mw WW xhh Start of line Start of string End of line End of string Word boundary Not word boundary Start of word End of word Control character White space Not white space Digit Not digit Word Not word Hexadecimal character hh Octal character xxx POSIX Character Classes upper lawer alpha alnum digit xdigit punct blank 5pace entrl graph print word Assertions Ta 7 l
140. to change its description Ok Cancel FIGURE 8 18 Enter a description Window Once finished click the Ok button to run the PHR report 101 ARMEDXPERT USER S MANUAL The PHR report shows the PHR at each Locus for each sample chosen PHR Report o ES pss1179 p21511 75820 csriPo 0351358 135317 0165539 251338 195433 vwa 18551 Amel 055818 Fea 1 5 Dilution PHR Average 1 5 Dilution ID A 3 l 5ng 6 HO2 0 8652 0 8369 0 8669 0 7897 0 78277 oss 0 9622 0 6037 0 9862 0 9246 09320 ID A2 15ng 2 A02 0 9716 0 9629 099861 0 9570 0 9609 0 9429 0852 0 9716 0 9710 0 9232 S 8000 iD A2 15 3 801 99652 0 9595 ossr 99004 ose osos 0 9452 08561 0 9530 osseo 09353 25999 iD A3 15ng cox osese _0 8422 ose ozsa 0 7971 ose osz9 0 6117 oseso 0 9333 0592 ouooe Pus std Deviation 0 0568 0 0611 sbrv o 0 0707 0 0770 0 0906 soiv o spiv o sorv o 0 0334 oiv o 0 0679 01269 0 0788 0 0194 0 0136 00000 Min 0 8652 0 8369 0 0000 0 8438 07841 0 7827 0 0000 0 0000 0 0000 0 9067 0 0000 o s093 0 6037 0 7957 0 9246 0 9232 Max 0 9716 0 9629 0 0000 0 9861 0 9570 0 9667 0 0000 0 0000 0 0000 0 9840 0 0000 0 9799 0 8561 0 9862 0 9710 0 9592 std Error 0 0254 0
141. to show on the report and the minimum number of included matches To show all included set the minimum number of included to 1 as shown below Click the Match button to run the report A report like the one in Figure 8 8 will appear Min no exact matches 6 Values are inclusive Use 1 to match any amount Min no included 1 Match Cancel FIGURE 8 8 Samples Comparison command window c3 s 25 DEM 2 Component 4 Vict Mix Stains Analyst txt M Staff Min no exact matches 6 Values are inclusive Use 1 to match any amount Min no included 1 y Match Cancel FIGURE 8 9 Samples Compatison command window example 95 ARMEDXPERT USER S MANUAL The Report lists the Reference Samples in the first column The second column list all matched comparison samples The third column lists the number of exact matches equal to or greater than the user defined amount The final column lists the number of included matches Reference Sample Comparison Sample No Exact Matches gt 6 No Included 06 06 20 F0 Ladder Identifiler 06 06 20 A03 Ladder Identifiler 06 06 20 A01 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 D01 XX0156 3 99XX9999Q1 15 2F3 Shirt Vict 06 06 20 E01 XX0159 1 99XX999901 15 8F1 Shirt Vict 06 06 20 F01 XX0161 1 QA003F1 06 06 20 H01 XX0162 1 Reag BlankF1 06 06 20 B02 XX0162 2 Reag BlankF2 06 06 20 C02 XX0164 99XX9999Q1 2 LFS Vict 06 06 20 D02 XX0165 QA004
142. uency calculations separated by Caucasian African American and Hispanic respectively will be listed under each allele The probability of exclusion and probability of inclusion will be listed to the right of the screen also separated by ethnic group Probability of Inclusion XX0156 1 99XX999901 15 2F1 Shirt Vict 06 06 20 D01 c 5 55 XX0156 1 99XX9999Q1 15 2F1 Shirt Vict 06 06 20 DO1 amp Locus D85179 25 O 021511 28 29 30 D75820 9 10 9 CSF1PO 0351358 15 16 6 93 0 9541 0 0459 v FIGURE 7 45 Probability of Inclusion Page 86 ARMEDXPERT USER S MANUAL The calculations by ethnicity for the entire profile will appear at the bottom of the page The calculations are shown in both exponential and alphanumeric form 2225 21 222 24 25 Caucasian Lin 3 9534E 7 39 5 Million Black lin 1 8501E 9 1 85 Billion Hispanic Lin 2 9496E 8 294 Million Sources FBI E aia FIGURE 7 46 Bottom of the Probability of Inclusion Page 87 ARMEDXPERT USER S MANUAL The fourth type of frequency calculations is a Mixture calculation It is used to perform frequency calculations on a two or three person mixture sample To obtain the Mixture Calculation page click on the Mixture button The Select Pick via mouse window will appear Select the sample you wish to perform the calculations on with your mouse ot click on the V arrow and s
143. utomatically Sort by total matches exact included C Sort by exact matches C Sort by included matches FIGURE 2 3 Match amp Compare Section of the General View options D851179 D21511 D7S820 CSF1PO D351358 THO1 D135317 Dit XX1747 99XX5678 K10 Susp 2 Ref 06 08 21 H03 XX1748 99XX5678 K11 Elim Ref 06 08 21 A04 XX72 1 99XX5678 Q1 2 F1 Vag Swab 06 07 14 C02 XX72 2 99XX5678 Q1 2 F2 Vag Swab 06 07 14 D02 XX74 1 99XX5678 Q1 F1 Gen Swab 06 07 14 A03 XX74 2 99XX5678 Q1 F2 Gen Swab 06 07 14 B03 m E 3913 7 iim L iu l 2932 nuj i11 136 w 11 10 11 12 1113 30302 910 10 12 XX95 99XX5678 K9 Susp 1 Ref 06 07 14 F07 FIGURE 2 4 Example of Reference at top option view Isun _o2isi1_ _p7sez0_ _cstapo mHor DING pie as Ba oa on u Jij a XX1747 99XX5678 K10 Susp 2 Ref 06 08 21 H03 XX1748 99XX5678 K11 Elim Ref 06 08 21 04 XX72 1 99XX5678 Q1 2 F1 Vag Swab 06 07 14 C02 XX72 2 99XX5678 Q1 2 F2 Vag Swab 06 07 14 D02 XX74 1 99XX5678 Q1 F1 Gen Swab 06 07 14 A03 XX74 2 99XX5678 Q1 F2 Gen Swab 06 07 14 B03 11 10 11 12 11 13 30 30 2 10 12 XX95 99XX5678 K9 Susp 1 Ref 06 07 14 F07 FIGURE 2 5 Example of the Reference at the bottom if option is not checked When a check appears next to each option that option is active Click on the box next to each option to re
144. y for each check is shown circled in red in each example Check Controls evaluates ladders controls and QA profiles to determine if they are within established parameters The ladders controls and QA profiles that are within established parameters are highlighted green The ones that are not are highlighted pink Once you click on the Check Controls button only the controls will appear on the table To bring the samples back in view either press the F8 button once and just the samples will be shown twice and both samples and controls will be shown or go back to the View tab and click on All or Samples depending on what you want to view The check control settings are set up under the Ladders and Controls subsection of the Quality Control section in the ArmedXpert Options Menu 5 ArmedXpert DEMO 2 Component 4 Vict Mix Stains Analyst txt Views Data QC Checks _ Match amp Comparison Interpretation Reporting 8 Ma Rie Y SMS x O Check Check dllele Stutter RFUs CODIS Allele Check OSD PHR Check Begin Clear Controls Ordering Alleles Count X multi Review Checks Standard Checks CODIS QC QC Review Clear Ladder Identifiler 06 06 20 A01 FALSE aa asas sas 1 19 Daie 87 7 Ladder Identifier 06 06 20 A03 Szafera 1213 es som sas miss foto un 6 7 fz Ladder identifier 06 06 2003 _ 585 19 85 15 16 9 10 11 11 67 7 06 20 H0 6 amp 9 1113 17 24

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