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User`s Manual YSI 1500 Sport

1. Laag GI CH CH CA Lei 5 io 5 S S ER D CH Qa i Lei Wei ER E Lei Zi CH Qa Inject the 5 mmol L standard When you remove the injection device you should see RUNNING STIR ON RUNNING REF ON WASHING If the probe current is outside the expected limits you will see ERROR MEMB SENS INJECT 5 mmol CALIBRATOR CHECK Inject the 5 mmol L standard to verify a successful calibration Now you should ee Dn RUNNING STIR ON RUNNING REF ON WASHING CAL CHECK XX XXmmol press MENU SAMPLE 0 CAL 1 PRIME 2 6 4 Press ENTER the display vvill read ENTER ID XXX VAIT INJECT SAMPLE Z ie ki J te pe Lei CH Kel fer CH Qa Lei io CH Lei Lei ti Z wel ti pe est D CH echt Lei ks S Lei pi CH Go echt Inject the sample When you remove the Syringepet or Injector you will see RUNNING STIR ON RUNNING REF ON XXX XX XXmmol L WASHING MM DD YY HH MM XXX XX XXmmol L The sample result will be displayed along with the ID in this case 000 date and time Linearity failure means that the instrument is not performing to specifications at the upper limit of the dynamic range This could be due to poor membrane performance check the date the membrane was installed to verify that it has not expired also verify that the membrane has not been subjected to
2. DISPLA Y 0 DELETE ALL 1 To select viewing the results on the display press ENTER LIST ALL 0 BY DATE 1 ID 2 6 19 6 20 The results that need to be deleted can be displayed by date by ID or all the results may be displayed To delete the displayed result press the DELETE key and the result will be deleted from the memory The display will then step to the next result If you do not wish to delete this result press ENTER and the next result will be displayed Deleting All Results RUN 0 RECALL 1 SETUP 2 DIAG 3 Q Geh ie D E S CH 5 S Lei io CH Lei Lei ae 5 oa ti zZ C th e Lei ba Nn Ch CH Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 To select vievving the results on the display press ENTER DISPLAY 0 DELETE ALL 1 To delete all of the stored results press 1 then ENTER ARE YOU SURE NO 0 YES 1 To delete all results press 1 then ENTER The display will read RESULTS DELETED PRESS MENU If you chose not to delete all the results pressing MENU will return you to the previous menu Pressing ENTER will display RESULTS OK PRESS MENU Press MENU to return to the Main menu 6 7 Daily Operational Checks To assure that your SPORT is operating properly check the membrane integrity and the linearity every day We recommend that you keep an instrument logbook in which dates and lot numbers of reagents are recorded along wi
3. 300 BAUD 0 1200 BAUD 1 Select the desired setting by number then ENTER The display vvill return to the previous menu Lei CH GH Ch Q S on so ct CH o Lei ue Ki CH Lei Lei Fa 5 gq a gt CH 5 tH zZ ti Ke DATA 0 BAUD 1 STOP 2 PARITY 3 Select stop bits by pressing 2 then ENTER STOP BITS 1 02 1 Select the desired setting by number then ENTER The display vvill return to the previous menu DATA 0 BAUD 1 STOP 2 PARITY 3 Select parity by pressing 3 then ENTER NONE 0 EVEN 1 ODD 2 HI 3 LO 4 Select the desired setting by number then ENTER The display vvill return to the previous menu Setting the LCD Contrast RUN 0 RECALL 1 SETUP 2 DIAG 3 H DATE 0 TIME 1 RS232 2 MORE 3 A LCD 0 RADIX 1 STIR 2 MORE 3 n n n Q Ei CH CH CH 2 amp Ka O S z e z J z 5 5 ER o Z E T ge O Km a e a S 2 Zo K lt 5 5 5 ko oa me be paj z s 2 SI da ti Pa z 5 lt S D tri ti Z Z Z c ti i e 5 5 5 Lei e for Lei CH CH LCD DARKER 0 LIGHTER 1 The contrast can be adjusted up and down by pressing the appropriate number and then the ENTER key The adjustment vvill increment vvith each pressing of the ENTER key Press MENU until the Main menu reappears Displaying Numerical Values in RADIX The default setting shows a period separating the integer part of the number fr
4. CORRECTIVE ACTION Clean probe surface SECTION 8 5 POSSIBLE CAUSE Temperature probe auxiliary electrode surface fouled CORRECTIVE ACTION Clean probe surface SECTION 8 5 POSSIBLE CAUSE Main power disruption CORRECTIVE ACTION Enter PROBE diagnostics and check probe current SECTION 7 1 ERROR MEMB SENS PRESS MENU This fault is generated only during the Calibration cycle Because a sample of a known concentration 5 mmol L Lactate Standard has been injected the current generated should fall within an expected range 5 45 nA If the actual current is higher or lower than expected the above message will be displayed POSSIBLE CAUSE Improper sample injection CORRECTIVE ACTION Repeat procedure SECTION 4 1 POSSIBLE CAUSE Failing enzyme membrane CORRECTIVE ACTION Perform daily operational checks and replace if necessary SECTION 6 7 POSSIBLE CAUSE Pinched plugged leaking or disconnected tubing CORRECTIVE ACTION Fix or replace tubing SECTION 8 6 POSSIBLE CAUSE Calibrator solution out of spec contaminated or in use for more than 30 days CORRECTIVE ACTION Replace Calibrator solution and repeat procedure POSSIBLE CAUSE Enzyme probe surface fouled CORRECTIVE ACTION Clean probe surface SECTION 8 5 POSSIBLE CAUSE Temperature probe auxiliary electrode surface fouled CORRECTIVE ACTION Clean probe surface SECTION 8 5 POSSIBLE CAUSE Stir bar not present CORRECTIVE ACTION Disassemb
5. We recommend at this point that you contact YSI Customer Service as this is a system failure that may require returning the instrument The second option is to select YES which will reformat the SPORT memory The software will attempt to correct itself but in doing so ALL stored data will be lost If YES was selected the stir speed date and time must also be reset Refer to Section 3 Instrument Setup for more information if necessary IMPORTANT Any previously stored sample results will be permanently erased by selecting YES BAD RAM DATA PRESS ENTER A check is done of all stored data after each sample cycle if there is a discrepancy this message will be displayed OK TO LOSE DATA NO 0 YES 1 Two options exist at this point one is to select NO and save your data and decide not to continue Choosing this option will display the following POWER DOWN CONSULT MANUAL We recommend at this point that you contact YSI Customer Service as this is a system failure that may require returning the instrument The second option is to select YES which will reformat the SPORT memory The software will attempt to correct itself but in doing so ALL stored data will be lost If YES was selected the stir speed date and time must also be reset Refer to Section 3 Instrument Setup for more information if necessary IMPORTANT Any previously stored sample results will be permanently erased by selecting YES INJECT TIMEOUT PRESS MENU Thi
6. e Printer A print test form is sent over the infrared data link e Keypad Each key can be verified for performance e Display A diagnostic routine checks every LCD segment for failure e Battery Battery voltage can be monitored on the display 7 1 Checking Probe Current Go to Main menu by pressing MENU until you see RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Diagnostics by pressing 3 then ENTER PROBE 0 TEMP 1 INJ SW 2 MORE 3 Select Probe by pressing ENTER PROBE 0 REF 1 BUF 2 To display the probe current press ENTER The display vvill read 7 1 WAIT PROBE X XX nA The display will update every few seconds To leave this condition press MENU The display will read PROBE 0 REF 1 BUF 2 You can also run either the reference or buffer pumps and monitor the change in probe current To do this press the desired numerical key and then ENTER the display vvill read PROBE X XX nA The display will update every few seconds To leave this mode press MENU PROBE 0 REF 1 BUF 2 as CH Lei Lei ti zZ C be i et F S i i CH i 5 S Oo s 7 2 Checking Sample Chamber Temperature RUN 0 RECALL 1 SETUP 2 DIAG 3 Q eri D 5 si CH 5 S Lei io CH Lei Lei ae 5 oa ti zZ C cr e Lei S Lei Ch CH 2 N CH CH Q lt lt J job 09 O n E Q n ST Ne ke CH n n i gq LA ot D CH j ti
7. A 5 S CH 5 c o Ka us CH Lei Lei Se 5 ga ti zZ C ci or S e c Lei CH CH Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 H LCD 0 RADIX 1 STIR 2 MORE 3 N CH GH Ch Q O CH o Lei ke CH Lei Lei 4 gq LA ot D CH tr Z m ai UNITS 0 MODE 1 SET DEFAULT 2 Lei CH SZ Ch O of O CH S Lei io CH Lei Lei 2 i oa LA am D CH 5 ti Z wel ti a Select Units by pressing ENTER UNITS mg dL 0 mmol L 1 Select mg dL by pressing ENTER or select mmol L by pressing 1 then ENTER Press MENU until the Main menu appears Selecting the Operational Mode Go to Main menu by pressing MENU until you see RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 Select More by pressing 3 then ENTER LCD 0 RADIX 1 STIR 2 MORE 3 H UNITS 0 MODE 1 SET DEFAULT 2 Lei CH Ku Ch O cr O CH S sn io CH Lei Lei Ge i oa LA yk D CH 5 ti Z wel ti a Lei CH Ch QO ech ie Q CH o Lei io CH Lei Lei pmi 09 a gt CH 5 tH Z ti Ke 3 3 15 SET CAL MODE AUTO 0 MANUAL 1 Select Auto mode by pressing ENTER or select Manual mode by pressing 1 then ENTER IMPORTANT After pressing 1 then ENTER to select Manual mode the cursor returns to its default location under the 0 however
8. ROM IS NEW PRESS ENTER J S e D Loi lt a ao CH B PTj S c gt a An internal test of the most recent and current EPROM checksum data is done and if there is a discrepancy this message is displayed This fault is generated when the software has been replaced with a different version Pressing ENTER will display the following message OK TO LOSE DATA NO 0 YES 1 Two options exist at this point one is to select NO save your data and decide not to continue Choosing this option will display the following POWER DOWN CONSULT MANUAL We recommend at this point that you contact YSI Customer Service as this is a system failure that may require returning the instrument The second option is to select YES which will reformat the SPORT memory The software will attempt to correct itself but in doing so ALL stored data will be lost If YES was selected the stir speed date and time must also be reset Refer to Section 3 Instrument Setup for more information if necessary IMPORTANT Any previously stored sample results will be permanently erased by selecting YES BAD SYS VARS PRESS ENTER A check is done of all data system variables after each sample cycle if there is a discrepancy this message will be displayed OK TO LOSE DATA NO 0 YES 1 Two options exist at this point one is to select NO and save your data and decide not to continue Choosing this option will display the following POWER DOWN CONSULT MANUAL
9. STIR 0 PUMPS 1 PRINT 2 MORE 3 3 TEST PRINT 0 TEST RS232 1 n n n EE Di CH CH CH Q Q Q et et 7 3 E 5 SA CR 2 ke fe 6 CH e Lei Pa 3 Co A S CN Si Z ga 09 N Ww Q ot Kai S E 5 5 tr B L z z o Z gt vd si tri 5 ti Paj S el ti ei Press 1 then ENTER to send a test data string over the RS232 data link XMITTING XXXXXXXXXXXXXX TEST PRINT 0 TEST RS232 1 gol CH Lei Lei ti zZ C ct ie CH ce E 5 5 ct O GE D CH e i CH i 7 8 Testing the Keypad 7 6 RUN 0 RECALL 1 SETUP 2 DIAG 3 H PROBE 0 TEMP 1 INJ SW 2 MORE 3 Ee Q e Q aj J J O 5 Le CH a 5 Q gt Z ka i a CH Ce Lei Lei SE 5 5 tic 09 5 z Z 5 C E D 5 Z SI tri lt re o Lei CH CH Lei CH Ch e O CH S lt gr 3 CH Lei Lei e Do LA 5 CH 5 ti Z wel ti Ke STIR 0 PUMPS 1 PRINT 2 MORE 3 H KEYS 0 DISPLAY 1 VER 2 MORE 3 Lei CH Ch QO et ie CH ST Lei Wei CH Lei Lei 4 5 gq LA at eg CH 5 ti Z ti a Select Keys by pressing ENTER The display will be empty except for the cursor in the upper left hand corner The display will now respond to each key pressed by showing the description of the key on the display Press MENU to return to the Main menu 7 9 Testing the Display Go to Main menu by pressing MENU until you see RUN 0 RE
10. The loaded voltage will typically be about 0 06 volt less RUN 0 RECALL 1 SETUP 2 DIAG 3 7 8 Select Diagnostics by pressing 3 then ENTER PROBE 0 TEMP 1 INJ SW 2 MORE 3 Select More by pressing 3 then ENTER STIR 0 PUMPS 1 PRINT 2 MORE 3 Select More by pressing 3 then ENTER KEYS 0 DISPLAY 1 VER 2 MORE 3 Select More by pressing 3 then ENTER BATTERY 0 Press ENTER to display the non loaded battery voltage BATTERY XX XX XX The display will update every few seconds Press MENU to return to the Main menu The number on the bottom line is the number of times the battery voltage reading is updated and will clear automatically when leaving this menu Note Typically the loaded voltage at which the actual battery checks are done is lower by 0 10 volts 7 9 7 10 8 Maintenance Turn the instrument povver off during maintenance This vvill prevent any accidental activation of the fluid pumps which could cause spills or air in the tubing 8 1 Daily Maintenance Certain procedures are to be followed each day that the SPORT is in use Empty the Waste Bottles Sh WARNING The waste contents of the SPORT are considered a biohazard and must be disposed of accordingly The waste bottle should be cleaned with a disinfecting agent The reagents used in the instrument are non toxic and consist of a phosphate salt buffer lactate standards and the detergent Triton X 100 Check Buffer and Reference Bottle Level
11. isopropyl alcohol and then reagent grade water 3 Prepare a small amount of 0 5 sodium hypochlorite solution 4 Immerse the sensor in the solution for about 45 seconds 5 Remove the sensor and immediately rinse it with reagent grade water for at least 2 minutes 6 Install anew membrane and return the probe to service NOTE Several hours may be required for the sensor to stabilize after cleaning 8 6 Tubing Replacement The buffer and reference solutions are moved through the SPORT with peristaltic tubing pumps Tubing life depends on how much the instrument is used Replace all of the SPORT tubing at least every 6 months Any tube that becomes loose at the fittings should be replaced immediately Remove the screws holding the buffer and calibrator pump to the case panel Disconnect and remove all the instrument tubing New tubing is supplied in the Preventive Maintenance Kit Connect the new tubing as shown in Figure 8 1 When installing new pump tubing first thread the tube through the pump head and install the roller assembly see Figure 8 2 It will help to twist the roller assembly as you install it It should snap into place Remount the pump to the case panel Remember to install the tube bushings on both the inlet and outlet side of the pumps Figure 8 1 SPORT Tubing Routing A WARNING It is extremely important that the correct size tubing be used for the supply and waste pumps Switching the two will result in sampl
12. of battery only testing the next day The SPORT may be turned back on immediately or left with the power off The battery will be recharged in either condition Turning on the power will allow immediate continuation once the probe has restabilized ZN WARNING Always make sure the sport power is turned off prior to connecting the battery charger Failure to do this could result in stored data corruption if the battery has been discharged to a very low level Refer to Appendix F Temperature Effect on Operation for additional information 8 9 Storing the SPORT Electrical System We recommend that while storing the SPORT you leave the unit on charge This will guarantee that the next time the SPORT is needed the battery will be fully charged and ready for a full day of use if necessary Failure to do this could result in a battery that is incapable of retaining a charge sufficient enough to guarantee 8 hours of use This is because even though the battery is hardware disconnected at a level of 11 5 volts it is possible due to the normal discharging 8 6 of the battery itself that the capacity of the battery vvill be permanently compromised if the unit remains off charge for several months YSI recommends that prior to storing the battery should be fully charged 8 hours minimum and the power turned off Fluid System If the SPORT is to be inactive for longer than a week we recommend that the whole system be flushe
13. s surface and rotate the probe back and forth 4 Rinse the sensor with reagent grade water and return the sensor to normal service Sensor Cleaning and Reconditioning The following procedure is recommended to be used only when the enzyme sensor stops operating normally and the above maintenance procedure is ineffective This is not a routine maintenance procedure 1 With the membrane removed immerse the sensor in a 14 solution of ammonia for 3 minutes 2 Immediately after soaking rinse the sensor with reagent grade water for 3 5 minutes It is important that all the residual ammonia is removed 8 3 3 Prepare a small amount of 0 5 sodium hypochlorite solution 4 Immerse the sensor in the solution for 30 45 seconds Check periodically sensor should darken but do not allow it to become black 5 Remove the sensor and immediately rinse it with reagent water for at least 2 minutes 6 Install anew membrane and return the probe to service The procedure below is only recommended as a last resort for revitalizing a sensor after the above cleaning procedure has been unsuccessfully attempted Z WARNING The following procedure may cause permanent damage to the enzyme sensor 1 Using a clean new pencil eraser carefully rub the center electrode platinum of the sensor Do not rub the epoxy between the two electrodes or the outer silver electrode 2 To remove any eraser dust or residual rinse sensor with 70
14. the instrument is now in Manual mode Press MENU until the Main menu appears Resetting System Default Parameters If the system is reset all stored results will be erased time date and stir speed will have to be manually reset to correct settings and the analyzer will require recalibration The system will be reset to a series of default settings which are listed below RS232 Communications Baud rate 1200 Data 8 bits Stop bits 1 Parity None Mode Automatic Calibration Stir speed Maximum Memory Cleared All stored results deleted Radix Period Units mg dL Go to Main menu by pressing MENU until you see RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 H LCD 0 RADIX 1 STIR 2 MORE 3 gt UNITS 0 MODE 1 SET DEFAULT 2 Lo Lo CH CH CH CH Q Q O O CH CH o S s lt lt ge e CH CH n n n n ue juar je je gq gq LA LA ot ES D D CH CH 5 D D D Z Z el el ti ti SS SS Select Default by pressing 2 then ENTER ARE YOU SURE NO 0 YES 1 To set all system parameters to the default settings press 1 then ENTER The A FEW SECONDS DELAY UNITS 0 MODE 1 SET DEFAULT 2 et eg Lei se i lt z m CH a Press MENU to return to the Main menu 3 17 3 7 Preparing the Fluid System Once you have setup the electronics of the instrument filled both supply bottles and empt
15. zZ C E D cr Z e far Lei Ch CH Select Diagnostics by pressing 3 then ENTER PROBE 0 TEMP 1 INJ SW 2 MORE 3 H STIR 0 PUMPS 1 PRINT 2 MORE 3 Lei CH SZ Ch O E O Kei CH S Lei io CH Lei Lei can i Do LA et D CH 5 ti Z wel ti SS Select Pumps by pressing 1 then ENTER The display will read BUF OFF 0 ON 1 REF OFF 2 ON 3 Turn the pump motors on or off by pressing the appropriate number key and then ENTER Press MENU to return to the Main menu 7 6 Sending a Test Print String Over the Infrared Data Link RUN 0 RECALL 1 SETUP 2 DIAG 3 H PROBE 0 TEMP 1 INJ SW 2 MORE 3 S STIR 0 PUMPS 1 PRINT 2 MORE 3 Lei Lei Q o o et si S z S s 5 i o 8 t 2 S B O CH K i o A ei lt lt E lt 5 ce DT E E pl e LA 2 d Si E e f a w z Z g D S z tri B el D z SI ri Lei O x E Lei CH CH Select Print by pressing 2 then ENTER TEST PRINT 0 TEST RS232 1 Press ENTER to send a test form over the infrared data link PRINTING TEST PRINT 0 TEST RS232 1 I CH Lei Lei ti zZ C ct CH p 5 D et esh D CH D z CH i 7 7 Sending a Test Data String Over the RS232 Data Link RUN 0 RECALL 1 SETUP 2 DIAG 3 Q et ie e 5 S CH 5 S Lei io CH Lei Lei Ai 5 oa ti E C be Z Lei Lei Ch CH 7 5 H PROBE 0 TEMP 1 INJ SW 2 MORE 3 3
16. 5 Power If the SPORT is being powered up for the first time or it has been unused for some period of time the first thing you should do is connect the battery charger AN CAUTION Make sure the SPORT povver is turned off before plugging in the battery charger 3 6 In normal use you can leave the power on as long as you like provided the battery charger is connected Leaving the charger connected and the povver on vvill prevent the battery from discharging and requiring charging prior to the next usage If the SPORT is stored with the power on and the charger disconnected the battery will discharge to a level requiring a recharge within 3 days YSI recommends that the SPORT be used with the battery charger whenever AC power is available IMPORTANT Probe polarization will be disrupted when power is turned off Time for restabilization will be needed when power is restored Either of the following displays confirm correct power up RUN 0 RECALL 1 SETUP 2 DIAG 3 CONNECT CHARGER PRESS ON If the display prompts you to connect the charger you may do so at this time without turning off the power switch The software will prohibit running until the battery has been sufficiently recharged Refer to Section 9 Troubleshooting for more information If neither of these displays become active press the Reset pushbutton located on the side of the unit If the SPORT fails to respond turn off all power and call YSI Technical Supp
17. 60 10 62 07 180 16 30 03 34 02 149 21 137 14 69 00 160 11 176 12 180 16 180 16 215 64 282 12 168 11 153 14 124 22 124 14 130 12 110 11 69 49 137 14 78 13 181 07 65 12 166 01 97 18 205 64 240 18 128 15 76 12 285 22 107 16 Lactate Interfering Level mg dL 15 2606 1 1 4376 12 108 1800 8 0 3 1 13 4 3 1 6 12 1 0 2 9 209 107 192 3 2 3 60 mmol L 1 0 868 0 32 293 0 88 15 102 0 52 0 25 1 0 0 39 0 23 0 88 0 13 0 165 12 11 9 3 0 04 0 42 34 14 5 14 6 15 Appendix F Temperature Effect on Operation Both the measurement chemistry and the electrical performance have very predictable reactions to changes in ambient temperatures To correct for these changes a temperature compensation algorithm has been included in the result calculation however to guarantee accurate results we compensate only over a limited range It may be possible that in some situations the 1500 SPORT may appear to be overly sensitive to TEMPERATURE STABILITY ERRORS If that is the case there are simple precautions that should be taken to insure the quality of the results Whenever possible the 1500 SPORT and the fluid supplies should be equilibrated to the ambient temperature of the actual testing site and then calibrated This is quite easily done when testing indoors but requires special effort if done outdoors When testing outdoors the 1500 SPORT should be kept out of direct sunli
18. Appendix D Sample Preservation It is possible to collect samples for later analysis if the situation dictates it For this application the YSI 2315 Preservative Tube Kit is available The kit consists of re sealable tubes containing a preservative powder and packets of YSI 1515 Cell Lysing Agent ZN WARNING You must use the YSI Cell Lysing Agent in your SPORT when running samples preserved in YSI preservative tubes You cannot use the YSI preservative tubes unless the lysing agent is used in the instrument when the actual measurement takes place This is due to the fact that the preservative tubes contain a detergent This detergent when introduced into a system without the cell lysing agent can affect the membrane response and cause incorrect readings While this detergent is also present in the cell lysing agent the response effect is compensated for during normal calibration and the sudden introduction of a sample containing the detergent will have no adverse effect Refer to Appendix E Effects Of Selected Substances A sample size of 100 microliters minimum is required for each tube Samples preserved in YSI preservative tubes can be stored for up to 48 hours 25 C and for up to 7 days 5 C 13 1 13 2 14 Appendix E Effects Of Selected Substances Paracetamol Tylenol Acetaminophen can be a significant interference in overdose situations Effective therapeutic levels of Paracetamol in blood 0 07 0 14 mmol L are
19. Buffer Pump then flushes the chamber for approximately 33 seconds and the sensor response decreases to the normal baseline value During each subsequent sample cycle the reference solution is again measured and then internally compared to the reference solution concentration measured during the initial calibration If a variation exists the calculation of the sample concentration will be compensated accordingly The profile of probe current response is shown in Figure 5 3 Auto Mode and Figure 5 4 Manual mode AUTOMATIC CAL MODE i LINEARITY BOs rea ey E ide abet e DEE e SUBSTRATE CONCENTRATION TIME Injection prompted measure baseline current Inject 25 microliters of calibration standard Measure calibrator current and deduct baseline current Measure reference solution current and deduct baseline current Calculate calibrator to reference solution ratio and save Flush chamber with fresh buffer solution 5 minute window allowed for making cal check injection Injection prompted measure baseline current Inject 25 microliters of calibration standard Measure sample calibrator current and deduct baseline current Measure reference solution current and deduct baseline current Calculate sample calibrator to reference solution ratio Divide by original calibrator to reference ratio multiply by unit constant 5 Display result and flush chamber with fresh buffer No fixed requirement for checking lineari
20. ENTER TIME HH MM SS XX XX XX The first and second digits that you press define the desired hour 06 is 6 am and 18 is 6 pm The third and fourth digits you press define the minute you can use anything between 00 and 59 The fifth and sixth digits define the seconds If the time you see on the display is what you want press ENTER Example Nine thirty in the morning would be entered as 09 30 00 ENTER TIME HH MM SS 09 30 00 If you have entered an impossible time 99 00 00 for example the display will read TIME PRESS ENTER 99 00 00 Press ENTER to return to the previous menu for another attempt to enter the time If the time of day you have entered is accepted press MENU until you return to the Main menu RS232 Communication It is possible to send all of the stored sample data to a personal computer but in order to do so a communication software package is required to be installed in the PC Once the communication package is installed in the PC itis simply a matter of making sure the settings of the PC and the SPORT are the same The SPORT is flexible and allows many choices RUN 0 RECALL 1 SETUP 2 DIAG 3 Q O ct D G 5 5 CH 5 E o te us CH Lei Lei 2 5 ga tH zZ C ci cr S lt c Lei CH CH Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 Select RS232 by pressing 2 then ENTER DATA 0 BAUD 1 STOP 2 PARITY 3 DATA 0 BAUD 1 STOP 2 PARITY 3
21. MM DD YY HH MM XXX X XXmmol L Every time you press ENTER the next value stored will be displayed You can cycle step by step through all of the results stored in memory The date time ID number will change as necessary to match each sample When all of the results have been displayed you will see this display NO SAMPLES PRESS MENU Z OTE It is possible to end the result recall at any point by pressing MENU Recalling Today s Sample Results Go to the Main menu by pressing MENU until the display reads RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Recall by pressing 1 then ENTER 6 11 6 12 SAMPLES LCD 0 PRINT 1 XMIT 2 To select viewing the results on the display press ENTER DISPLAY 0 DELETE ALL 1 To select viewing the results on the display press ENTER LIST ALL 0 BY DATE 1 ID 2 H DATE MM DD YY XX XX XX Lei CH CH el A J CO CH o Lei We ki CH n n pm i 09 E D CH 5 ti Z wel ti ai NOTE The current date is what will appear on the display Press ENTER The first sample result stored today vvill appear MM DD YY HH MM XXX X XXmmol L Every time you press ENTER the next value stored will be displayed You can cycle step by step through all of the results on this date The ID number will change as necessary to match each sample When all of the results have been displayed you will see this display NO SAMPLES PRESS MENU NOTE It is possible to end the resul
22. Manual Mode Probe Current Profile 5 4 Baseline Stability The SPORT checks the probe baseline current for stability whenever it enters the Sample mode The baseline current must be within an acceptable range less than 6 nanoamps in order for the instrument to perform to specification If the baseline current is greater than 6 nA an error message will be displayed see Section 9 Troubleshooting 5 5 Calibration The SPORT uses one lactate standard YSI 2327 5 mmol L for calibration and two lactate standards are available for checking linearity YSI 2328 15mmol L and YSI 1530 30 mmol L The following is the recommended calibration frequency Auto Mode e Every day at the beginning of testing e Whenever you change the membrane or install new reagent fluids e Whenever you turn the instrument power on e Every two hours while testing e Whenever you change injection devices Manual Mode e Every day at the beginning of testing e Whenever you change the membrane or install new reagent fluids e Whenever you turn the instrument power on e For maximum accuracy after every fifth sample e Whenever you change injection devices MANUAL CALIBRATION METHOD CALIBRATION USING REFERENCE SOLUTION Ah x K X which is equivelent to BC A Ci BY Vaj Net Sample Current Net Calibrator Current Unit Constant S i Sample Concentration w Kee A j Net Sample Current Net Calibrator Current Ja Net Refere
23. POSSIBLE CAUSE Tubing worn or stretched at connections CORRECTIVE ACTION Replace tubing SECTION 8 6 POSSIBLE CAUSE Air bubble stuck to stir bar CORRECTIVE ACTION Go to stir bar setup and increase speed until the stir bar tumbles See below Reset spin speed SECTION 3 6 The stir bar is driven by a spinning magnetic coupling if the speed is increased the coupling will be interrupted and the stir bar will tumble and vibrate fairly violently If bubble will still not release take a small diameter pin or other device paper clip works well and stick it into the sample chamber through the injection port and interrupt the stir bar motion The bubble should release itself at this point POSSIBLE CAUSE Buffer and or Reference tubing line in pumps crimped shut CORRECTIVE ACTION Disassemble pumps and open tubing or replace if more than 6 months since last replaced SECTION 8 6 Buffer and or Reference pump fail to pump fluid POSSIBLE CAUSE Tubing line in pumps crimped or stuck shut CORRECTIVE ACTION Disassemble pumps and open tubing or replace if more than 6 months since last replaced SECTION 8 6 POSSIBLE CAUSE Supply line blocked CORRECTIVE ACTION Clear tubing or replace SECTION 8 6 POSSIBLE CAUSE Supply line check valve stuck open CORRECTIVE ACTION Flush check valve or replace POSSIBLE CAUSE Bottle vent plugged causing negative pressure in the supply bottle CORRECTIVE ACTION Loosen
24. TX 100 LACTATE De et YA we J x AA gee a al oa K n qe S Ai A Ee K a Ae AH ae l 20 6 0 10 0 14 0 18 0 22 0 26 0 30 0 YSI 2300 mmol l Y 0 9893X 1559 DATA Plasma Lactate Without YSI 1515 cell lysing agent added to the buffer solution of either instrument Concentration 0 10 mmol L 10 20 20 30 Pooled Est Std Dev Mean Bias 0 1 mmol L 0 1 mmol L 0 2 0 1 0 3 0 1 11 Appendix B Sample Collection and Handling The YSI 1500 SPORT is not intended for home use Proper sample collection and preparation by a professional are vital factors in obtaining reliable results vvith the YSI SPORT This section covers the basic guidelines for collecting and preparing whole blood plasma and serum samples Much of the Blood Plasma and Blood Serum material is abstracted from Workshop Manual of Methods for the Determination of Glucose by Gerald R Cooper M D Ph D published by the Commission on Continuing Education Council on Clinical Chemistry American Society of Clinical Pathologists 1966 Blood Plasma Be sure that all collection equipment is sterile and chemically clean Use aseptic techniques in handling the sample from collection through assay During or immediately after collection mix the specimen with an anticoagulant and preservative to prevent coagulation inhibit enzyme activity and prevent bacterial growth Separate the red blood cells by centrifugation within 3
25. contacts the immobilized enzyme lactate oxidase it is rapidly oxidized producing hydrogen peroxide Reaction 1 The hydrogen peroxide H O is in turn oxidized at the platinum anode producing electrons Reaction 2 A dynamic equilibrium is achieved when the rate of H O production and the rate at which H O leaves the immobilized enzyme layer become constant Equilibrium is indicated by a steady state response The electron flow is linearly proportional to the steady state H O concentration and therefore to the concentration of lactate 5 1 5 2 Reaction 1 lactate Lactate O gt gt Pyruvate H O Reaction 2 HO gt 2H O 4 2e Pt anode The platinum electrode is held at an anodic potential and is capable of oxidizing many substances other than H O To prevent these reducing agents from contributing to sensor current the membrane contains an inner layer consisting of a very thin film of cellulose acetate This film readily passes H O but excludes chemical compounds with molecular weights above approximately 200 The cellulose acetate film also protects the platinum surface from proteins detergents and other substances that could foul it However the cellulose acetate film can be penetrated by such compounds as hydrogen sulfide low molecular weight mercaptans hydroxylamines hydrazines phenols and anilines Certain detergents endogenous and exogenous substances preservatives and reducing agents can in
26. instrument must be returned to the YSI Repair Center Field service is not available on this instrument We strongly recommend that you use the original shipping container for this purpose as it was designed to protect the instrument during transport Reagents and injection devices for the SPORT analyzer are not packaged in the same carton as the instrument Reagents come in a starter kit YSI 1504 and injection devices YSI 1501 or 1502 must be ordered separately ZA WARNING Always make sure the SPORT power is turned off prior to connecting the battery charger Failure to do this could result in stored data corruption if the battery has been discharged to a very low level 3 1 3 2 Component ldentification This section names the principal parts and describes their use Figure 3 1 and Figure 3 2 identify each part i BUFFER BOTTLE Pa DISPLAY INTERFACE PANEL LOCATED THIS SIDE BUFFER PUMP KEYPAD _ INJECTION POR ENZYME PROBI AND MEMBRANE SAMPLE CHAMBER E REFERENCE BOT EMPERATURE PROBE Figure 3 1 SPORT 3 2 The Buffer Reference and VVaste Bottles in the rear of the instrument are used to hold the solutions needed for sample analysis The Buffer Pump is a two channel peristaltic pump driven by a gearmotor One channel the lower body draws buffer from the Buffer Bottle and pumps it through the Sample Chamber The second channel the upper body draws fluid from the waste chamber and pump
27. not a problem The pharmacokinetic literature suggests that one would have to injest more than 3 times the recommended therapeutic dose in order for the plasma levels to be greater than 0 17mmol L Paracetamol interferees with the SPORT measurement because it can penetrate the cellulose acetate portion of the enzyme membrane and is oxidizable at the platinum eletrode at the applied polarization voltage The presence of elevated levels of Paracetamol in a sample results not only in an elevated lactate reading but also a ramping signal in the time region in which a steady state signal is normally observed ZN VVarning The follovving preservatives interfere vvith the measurement and should not be used Phenol Benzalkonium Chloride Methyl Paraben Per chloric Acid Sodium Azide Thymol Trichloracetic Acid Several classes of chemicals can damage the YSI sensor system or cause erroneous readings Most of these have no place in diagnostic medicines but may be of scientific interest Some substances such as triglycerides which are interferences for photometric sensor systems do not interfere with the SPORT electrochemical sensor system Reducing Agents Many reducing agents would give rise to a false signal current and falsely elevated reading if they succeeded in reaching the sensing anode of the YSI probe Most of these are excluded from the probe by the cellulose acetate layers of the membrane However thymol phenols anilines hyd
28. the cause of an instrument failure The first step is to accumulate as much pertinent information as possible e Review the printed reports for trends in data and errors This is only possible if the optional printer is being used while running samples Refer to Section 3 8 Printer Option for an explanation of the printed format e Check reagent and membrane installation dates Compare the elapsed times with the recommended times e Look and listen for problems unusually noisy components loose connections fluid leaks air bubbles in the sample chamber salt build ups etc e Review Section 7 Instrument Diagnostics to become familiar with testing individual components of the SPORT e Use the troubleshooting chart in this section to assist you in identifying the problem then use the chart to guide you to a corrective action If the problem cannot be resolved contact YSI Customer Service address and phone number in Appendix I Warranty and Shipping Information When communicating with service personnel please indicate the serial number of the instrument and if necessary the accessory products If writing or transmitting a FAX for assistance please include a thorough description of the problem s and copies of the printed reports if possible If it becomes necessary to return the instrument to YSI for servicing please refer to Appendix I VVarranty and Shipping Information for instructions 9 1 9 1 Fault Messages 9 2
29. 0 minutes of collection If delays between collection and assay are unavoidable follow one of these procedures 1 Refrigerate the whole blood at 4 C This is acceptable for periods up to four hours 2 Or refrigerate the plasma with preservatives at 4 C for up to three days Plasma with preservative left at room temperature may keep up to four hours 3 Or freeze the cell free plasma with preservative at 10 C or colder Plasma will keep indefinitely at this temperature Thaw the frozen plasma rapidly at 25 C and analyze promptly The freeze thaw cycle should not be repeated Tubes containing cells should never be frozen NOTE Chilling or freezing may precipitate aggluten or fibrinogen masses which may not redissolve In such cases remove them by centrifugation before assay Several common anticoagulants are suitable for use with specimens being prepared for assay These include Potassium oxalate in a concentration of 2 mg ml of blood Sodium fluoride in a concentration of 5 10 mg ml of blood EDTA in a concentration of 1 mg ml of blood Sodium fluoride and oxalate in combination in a total concentration of 2 mg ml of blood Heparin sodium as commonly supplied in Vacutainer collection tubes ZN WARNING The YSI SPORT is not suitable for use with samples containing thymol or other phenol preservatives These are interfering substances Oxalate anticoagulants and particularly the combination of sodium fluoride a
30. 0 G 140 L ag ei 12 0 3 ye 10 0 Ba 8 0 ai H 60 BD T F 40 E H K 20 bai 00 I I I I I I 20 6 0 10 0 14 0 18 0 22 0 26 0 30 0 YSI 2300 mmol l Y 1 0078X 0714 DATA Total Blood Lactate YSI 1515 cell lysing agent added to the buffer solution in both instruments Concentration Pooled Est 0 10 mmol L 0 1 mmol L 10 20 0 3 20 30 0 5 Std Dev Mean Bias 0 1 mmol L 0 3 0 2 10 3 10 4 30 0 28 0 26 0 24 0 22 0 1500 SPORT mmol l 20 0 Concentration 0 10 mmol L 10 20 YSI 1500 vs 2300 WHOLE BLOOD SYRINGEPET NO TX 100 LACTATE P KA Pa S P ag BO E i ra I I I f I I I I I I YSI 2300 mmol l Y 1 0076X 1302 DATA Whole Blood Lactate Extracellular ONLY Pooled Est Std Dev Mean Bias 0 1 mmol L 0 1 mmol L 0 3 0 2 0 4 0 3 20 30 1500 SPORT mmol l 30 0 28 0 26 0 24 0 6 0 40 20 0 0 YSI 1500 vs 2300 PLASMA SYRINGEPET 0 25 TX 100 LACTATE li 14 0 18 0 YSI 2300 mmol l Y 0 9793X 1345 22 0 26 0 30 0 DATA Plasma Lactate YSI 1515 cell lysing agent added to the buffer solution in both instruments Concentration 0 10 mmol L 10 20 20 30 Pooled Est Std Dev Mean Bias 0 0 mmol L 0 1 0 4 10 5 10 6 30 0 28 0 26 0 24 0 1500 SPORT mmol l 20 0 80 6 0 40 20 0 0 YSI 1500 vs 2300 PLASMA SYRINGEPET NO
31. 1 Sal Sensor Technology inic enini hh eds 5 1 5 2 OT e BEE 5 3 5 3 Measurement Methodology ni dar hg canine EE 5 4 54 Base lime SADI EE 5 6 T S Galibrallon skal ie E siete eae ee awed tee tea dete ahaa eed 5 7 9 0 EMG arity nd 5 8 Sa Temperatire Re EE EE 5 9 5 9 SoftWare EEN 5 9 Microprocessor EK E 5 9 Electrode Cheeks gc ciei tied cece ccegued ses sinisiin eiieeii EEE E ESS Ea 5 9 Temperature Error E 5 10 Battery Level CSR dare mend cca ac tou cu putts uc tasle ozs aus 5 10 1500 SPORT Menu Plow Chat cist sshivesse dit ve sdin rand 5 11 Instrument Operation since sesoiroi soon ne cante dE 6 1 DI Calibration Cycle kind ins imin dek sis den 6 1 Calibration Automatic Mode sajesa jav s do eisai deed 6 1 Calibration Manual EE ENEE 6 5 02 Sample EE 6 8 6 3 Recalling Stored Results iniscan e EEE AS 6 10 Recalling All Sample Results isen ioanen e nat vetoes a E ER 6 10 Recalling Today s Sample Results js sms ie shtekasdt visse Ee duo bin huas she duke kredo keste beat 6 11 Recalling a Result From Any Previous Date sauna aaa aaa aaa na aaa anen even even eee vene 6 12 Recalling a Result By ID tree Aere 6 14 6 4 Primtin EE 6 15 Printing All Sampl N SIS qish one den to gh e 6 15 Printime Todays EE 6 16 Printing Previous Days Jesus ieugbegt at nssdsjersekdsr khnsh 6 17 Printing Results By ID Number cist sdi tes dd nd 6 17 6 5 Transmitting Results Over the RS232 Communication Link a uuuua uuaa aaa aa
32. 1505 1506 1515 1530 015117 015118 2315 2327 2328 2329 2357 2363 2392 2751 Description 25 microliter Blunt Needle Syringepet 25 microliter Capillary Tube Injection Device 1500 Preventative Maintenance Kit 1500 L Lactate Starter Kit 2327 Lactate Standard 5 mmol L 1530 Lactate Standard 30 mmol L 2329 Lactate Membranes 2363 Potassium Ferrocyanide 2392 NaCl Solution 2357 Buffer Concentrate Kit 1505 Capillary Tubes 1515 Cell Lysing Agent Syringe 3cc 25 microliter Capillary Tube Package 100 count Hewlet Packard Infrared Printer HP 82240 Cell Lysing Agent 8 packs Lactate Standard 30 mmol L 125mL 120 VAC 60 Hz Battery Charger 220 VAC 50 Hz Battery Charger Preservative Tube Kit 1515 Cell Lysing Agent Preservative Tube Lactate Standard 5 mmol L 125mL Lactate Standard 15 mmol L 125mL Lactate Membranes 4 count Buffer Concentrate Kit 8 packs Potassium Ferrocyanide 125mL NaCl Solution 30mL Printer Paper 5 rolls box KE lt Qty 1 50 16 1 16 2 17 Appendix H Cleaning Disinfecting and Decontamination Sh Proper precautionary lab practices should be followed when handling biological hazards Suggested cleaning and disinfecting solutions e Isopropanol 70 e Sodium hypochlorite 5000 ppm free available chlorine 1 10 solution of house hold bleach Disassemble parts that require cleaning per instructions in Section 8 Maintenance Thoroughly clean with the disi
33. 4 10 Appendix A Typical Performance Data Testing of the YSI Model 1500 SPORT vs the YSI Model 2300 STAT suggests the follovving typical performance Capillary Tube Injector YSI Model 1502 30 0 28 0 26 0 24 0 22 0 1500 SPORT mmol l 20 YSI 1500 vs 2300 WHOLE BLOOD CAPILLARY 0 25 TX 100 LACTATE A MT EE p p t Es A a ef A 2 Ze A AH a K Di E K A A l l l l 20 6 0 10 0 14 0 18 0 22 0 26 0 30 0 YSI 2300 mmol l Y 0 9973X 0705 DATA Total Blood Lactate YSI 1515 cell lysing agent added to the buffer solution in both instruments Concentration 0 10 mmol L 10 20 20 30 Pooled Est Std Dev Mean Bias 0 1 mmol L 0 0 mmol L 0 2 0 1 0 4 0 0 10 1 10 2 30 0 28 0 26 0 24 0 1500 SPORT mmol l 8 0 6 0 4 0 2 0 0 0 Concentration 0 10 mmol L 10 20 YSI 1500 vs 2300 WHOLE BLOOD CAPILLARY 7 NO TX 100 LACTATE i de pA a Da t S pa a a JA Q p I I I I I i I I I I I YSI 2300 mmol l Y 1 0095X 0224 DATA Whole Blood Lactate Extracellular ONLY Pooled Est Std Dev Mean Bias 0 1 mmol L 0 1 mmol L 0 3 0 2 0 4 0 3 20 30 Blunt Needle Syringepet YSI Model 1501 YSI 1500 vs 2300 WHOLE BLOOD SYRINGEPET 1 0 25 TX 100 LACTATE 30 0 E ye 28 0 at eo 26 0 Es KE 24 0 at Es 22 0 E 1500 SPORT Be mmol 20 ye A 18 0 H vi AT 16
34. CALL 1 SETUP 2 DIAG 3 Select Diagnostics by pressing 3 then ENTER PROBE 0 TEMP 1 INJ SW 2 MORE 3 Select More by pressing 3 then ENTER STIR 0 PUMPS 1 PRINT 2 MORE 3 H KEYS 0 DISPLAY 1 VER 2 MORE 3 N CH GH Ch QO SH O Ki CH o Lei ke CH Lei Lei 4 5 ga LA m SE CH 5 ti Z wel ti e Press 1 then ENTER to initiate the display test routine Pressing CANCEL vvill halt the test at any time The test routine consists of displaying multiple character sets at three different contrast settings and also lighting every LCD segment Press MENU to return to the Main menu 7 1 7 10 Checking the Software Version Number It will be necessary to communicate the software version number to a service technician if a call is required Press MENU until you return to the Main menu RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Diagnostics by pressing 3 then ENTER PROBE 0 TEMP 1 INJ SW 2 MORE 3 F STIR 0 PUMPS 1 PRINT 2 MORE 3 Lei CH L Ch O er O CH o lt gr CH Lei Lei SCH i Do LA SE D CH 5 i Z wel ti e Select More by pressing 3 then ENTER KEYS 0 DISPLAY 1 VER 2 MORE 3 Select Version by pressing 2 then ENTER The softyvare version number vvill be displayed VERSION X XX LAC PRESS MENU Press MENU to return to the Main menu 7 11 Checking the Battery Voltage To display the non loaded battery voltage press MENU until you return to the Main menu
35. Disodium 216 4 DL Glyceric Acid hemi calcium 286 25 1276 9 6 Glycolic Acid 76 05 177 23 B Hydroxypyruvic Acid Lithium salt 110 DL B Hydroxybutyric Acid Sodium salt 126 4 Hydroxybutyric Acid Sodium salt 126 09 DL Malic Acid 134 09 DL a Hydroxybutyric Acid 126 09 84 6 7 L B Phenyllactic Acid 166 18 24 1 4 B Chlorolactic Acid 124 52 93 2 4 Oxalacetic Acid 132 07 Glycine 75 07 DL Alanine 89 09 Sdan B Alanine 89 09 SE DL a Hydroxycaproic Acid 132 16 45 3 4 Pyruvic Acid Sodium Salt 110 04 DL a Hydroxyisovaleric Acid 118 13 L a Hydroxyisocaprioc Acid 132 16 75 5 7 a Hydroxyisobutyric Acid 104 11 Glycerol 92 09 14 4 Substance Drugs Poisons and Miscellaneous Exogenous Substances Acetaminophen Metaphosphoric Acid Ethanol Methanol Iso Propanol Ethylene Glycol Acetylsalicylic Acid Formaldehyde Hydrogen Peroxide D Penicillamine Salicylamide Sodium Nitrite Sodium Salicylate Ascorbic Acid D Galactose D Glucose D Glucosamine HC1 Glucose 6 phosphate Monosodium Uric Acid p Aminosalicylic Acid 2 3 Dimercapto propanol Guaiacol Hydrazine Sulfate Hydroquinone Hydroxylamine Hydrochloride Isoniazid 2 Mercaptoethanol p Phenylenediamine HC1 Potassium Cyanide Potassium Iodide Potassium Thiocyanate Pyridoxine Hydro chloride Sodium Sulfide Nonhydrate 2 Thiouracil Thiourea o Tolidine Dihydro chloride o Toluidine Weight 151 17 46 07 32 04
36. E K 5 E S Lei Kei E S 2 D pj DO m LA oO Z si rd a LA Q m E Ke E B gt yp a ss L 2 D Q pri ko CT i 2 Kei E oO lt Ka 4 e S E E d S E a O er o MM DD YY HH MM XXX XX XXmmol L The sample result will be displayed along with the ID date and time Linearity failure means that the instrument is not performing to specifications at the upper limit of the dynamic range This could be due to poor membrane performance check the date the membrane was installed to verify that it has not expired also verify that the membrane has not been subjected to drying out as a result of no fluid in the sample chamber The acceptable ranges are 15 mmol 14 30 to 15 70 mmol 128 3 to 141 7 mg dL 30 mmol 28 50 to 31 50 mmol 256 5 to 283 5 mg dL If the result is not within these ranges replace the membrane allow time for it to break in and repeat the calibration process If the result is acceptable press MENU and the display will read SAMPLE 0 CAL 1 PRIME 2 You are now ready to measure samples 6 2 Sample Cycle With the SPORT calibrated as described you can proceed to measure lactate values in blood samples or plasma AN WARNING If you calibrate with the Syringepet measure with the Syringepet If you calibrate with the injector measure with the injector Otherwise you may get incorrect values REMEMBER The Reference solution is only used in Auto mode The f
37. Format Memory erase Set RS232 Default t Start Micro Clock j Stir Speed Maximum mg dL Figure 5 7 Menu Flow Chart 5 11 5 12 6 Instrument Operation IMPORTANT You should check the probe current before beginning tests See section 7 Instrument Diagnostics If there are any problems you don t want to discover them after you have begun Step by step instructions in this chapter will help you learn the operation of the SPORT Instructions for each step are followed by an illustration of the display as it should respond If the display does not respond correctly press MENU and try again It is not uncommon for an instrument to be unstable for the first hour or so once it has been initially setup and calibrated This is a result of the membrane requiring time to break in and also because the surface of the enzyme probe requires time to condition itself with respect to the new membrane and fluids As a result of this YSI recommends that in the first hour of use after initial setup the instrument should be checked several times for accuracy use YSI standards and calibrated as often as required 6 1 Calibration Cycle There are two modes of operation allowed on the SPORT Automatic and Manual mode Each have their own calibration process which are described in the sections that follow Calibration Automatic Mode In Automatic mode you should calibrate the SPORT on these occasions Every day at the beginning
38. H EN Inject the sample When you remove the Syringepet or Injector you will see RUNNING STIR ON RUNNING REF ON 6 9 If the concentration of the sample exceeds the measurable limits of the instrument the following message will appear ERROR OVERRANGE WASHING If you see this display no result will be stored or displayed press MENU and refer to Section 9 Troubleshooting If there is no problem the display will read XXX XX XXmmol L WASHING MM DD YY HH MM XXX XX XXmmol L The sample result will be displayed along with the ID date and time Press MENU to return to the Run menu The previous result ID date and time will be stored in memory until it is deleted SAMPLE 0 CAL 1 PRIME 2 6 3 Recalling Stored Results 6 10 The SPORT allows you to recall any stored result Results may be recalled by ID by date or all results may be recalled in chronological order The following sections identify the different vvays to do this Recalling All Sample Results Go to the Main menu by pressing MENU until the display reads RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 To select vievving the results on the display press ENTER DISPLAY 0 DELETE ALL 1 To select viewing the results on the display press ENTER LIST ALL 0 BY DATE 1 ID 2 Select List All by pressing ENTER Press ENTER The first sample result stored will appear
39. ION Enter PROBE diagnostics and cycle the buffer pump SECTION 7 1 POSSIBLE CAUSE Tubing routed incorrectly through peristaltic pumps CORRECTIVE ACTION Check tubing routing and verify fluid flow path SECTION 8 6 POSSIBLE CAUSE A sample has been injected into the sample chamber at the improper time CORRECTIVE ACTION Enter PROBE diagnostics and cycle the buffer pump SECTION 7 1 POSSIBLE CAUSE Pinched plugged leaking or disconnected tubing CORRECTIVE ACTION Fix or replace tubing SECTION 8 6 POSSIBLE CAUSE Buffer pump not running CORRECTIVE ACTION Enter PUMP diagnostics and verify buffer pump performance SECTION 7 5 POSSIBLE CAUSE Buffer pump not performing properly CORRECTIVE ACTION Replace tubing SECTION 8 6 POSSIBLE CAUSE Stir bar not present CORRECTIVE ACTION Disassemble chamber and reinstall stir bar SECTION 8 3 POSSIBLE CAUSE Stir bar not spinning CORRECTIVE ACTION Enter STIR diagnostics and check motor performance SECTION 7 4 POSSIBLE CAUSE Failing enzyme membrane CORRECTIVE ACTION Perform daily operational checks and replace if necessary SECTION 6 7 9 5 9 6 POSSIBLE CAUSE Newly installed enzyme membrane CORRECTIVE ACTION Enter PROBE diagnostics and check probe current SECTION 7 1 POSSIBLE CAUSE Newly installed enzyme probe CORRECTIVE ACTION Enter PROBE diagnostics and check probe current SECTION 7 1 POSSIBLE CAUSE Enzyme probe surface fouled
40. Ke SAMPLES LCD 0 PRINT 1 XMIT 2 To select vievving the results on the display press ENTER DISPLAY 0 DELETE ALL 1 To select viewing the results on the display press ENTER LIST ALL 0 BY DATE 1 ID 2 lt ei ga Z Lei CH Ch O E ke J S CH Lei Lei SC 5 N et ES CH 5 tH ti SS H ENTER ID XXX Enter the desired ID as a three digit number from left to right then press ENTER The first sample result stored in memory that has the number you ve entered will be displayed M DD YY HH MM XXX X XXmmol L Every time you press ENTER the next result stored will be displayed You can cycle step by step through all of the results with this ID The date and time will change as necessary to match each sample When all of the results have been displayed you will see this display NO SAMPLES PRESS MENU NOTE It is possible to end the result recall at any point by pressing MENU When you are finished press MENU until the Main menu appears 6 4 Printing Results It is possible to print out results as you are running samples This is possible because the SPORT sends all of the result data over the infrared data link automatically at the end of each sample cycle All that is necessary is that the printer be positioned correctly and turned on Refer to Figure 3 6 to determine printer positioning and alignment The following sections describe the actions required to print stored results Print
41. O Gabi 8 3 G20 Bin Replace Me bimor hesape ka kesi Ee e 8 4 8 7 Injection Port Seal Replac m nN La kgas vdriytonhi tue is dt kr din 8 6 8 9 4 R charsing he Batlery E EE 8 6 ee SOTEM M SO E 8 6 Troubleshooting neni does 9 1 9 Fault MESA S pas e sa 9 2 Dala System Fa liS ej sh eene e E AS 9 2 Battery Faults x ppm e 9 4 Enzyme Sensor PAWNS ehn eet dh se e qent 9 4 Temperature Tales pen kt 9 8 Fluid System AU 9 9 9 2 Possible Farl re Conditions sju parodi s e ar eea S 9 10 Appendix A Typical Performance Data naaaee ce vec co SE cc EE ce esese 10 1 Capillary Tube Injector YSI Model 1502 pun n e ht 10 1 Blunt Needle Syringepet YSI Model 1501 ng des ed kn 10 3 Appendix B Sample Collection and Handling cccscssscssscssscees 11 1 Blood Plasma oni ot id e eni dt N E N N T EE 11 1 Blood Seren ngas nenin 11 2 Whole Blood psh he 11 3 Appendix C Cell Lysing ssssessoseesesseosessessessossesesoeosessessessossesessessose 12 1 Using the YSI 1515 Cell Lysing A Sens eeh td des saadenccgeugiocva ecg batedadestecaandenevgnates 12 2 Buiter Solution uk 12 2 R terence Solution ane shine Da 12 2 Appendix D Sample Preservation scccccccscssscssscsesccssccssccsscessoees 13 1 Appendix E Effects Of Selected Substances due ce ce ce co SE ece eee ece seen 14 1 iii 15 16 17 18 19 R ducina AGED S ss 14 1 Calibration Shift by De tere emis roerei Eeer 14 2 Endogenous and Exogenous S
42. Partner4U BV Esdoornlaan 13 3951DB Maarn The Netherlands 31 0 6 516 536 26 2003 YSI Incorporated 015162 A15162H 2003 12
43. Preparation The blood from a finger or earlobe prick should be collected in a capillary tube If you use one of the YSI tubes which are preset to 25 microliters you must inject it with the capillary tube injector You can also draw venapuncture blood from a vial vacutainer or YSI Preservative Tube with the preset tube If the capillary tube has a capacity greater than 25 microliters the blood sample must be transferred to the YSI 1501 Syringepet for injection You can also use the Syringepet to sample and inject blood from a venapuncture vial or vacutainer The following sections instruct you in the use of both injection devices YSI Model 1501 25 microliter Syringepet The Syringepet can be used to extract a 25 microliter sample from either a vacutainer or a capillary tube of greater volume than 25 microliters see Figure 4 1 Begin vvith a sample volume greater than 75 microliters To remove any contaminants from the Syringepet you should rinse it three times with buffer or reagent quality water For maximum accuracy you should rinse it with buffer or reagent water at least twice and then with the sample twice before loading it for injection AN WARNING Precision and accuracy are dependent on sample loading technique Any air in the sample column will cause incorrect results Figure 4 1 1501 Syringepet Using the Syringepet may feel awkward at first but you must master the technique to get accurate results Practice injec
44. Y SI Life Sciences User s Manual YSI 1500 Sport L Lactate Analyzer Data SS life www YSI com Table of Contents 1 GOP h parane e A E TA 1 1 LI e EE 1 1 Introduction 2 au s k dedikon made ng Eed 2 1 EE d ka i ante BG aa dd da dt dt ENS A Ls Se a Seat 2 1 e E Te ET 2 2 INSTRUMENT Best ee ENEE u e is osasse 3 1 SS BT a 3 1 3 2 Compo ent demi E NEE 3 2 3 3 Reagent Preparation is tee 3 4 Aulomatic Medercher dag hs 3 4 AEN EI e e E ayssa iee 3 5 3 4 Membrane Installation EE 3 6 Did POWER EE 3 6 3 6 System Setups ye sea side EEN 3 7 EE 3 9 I e E ee elt ER OSPR ToT 3 10 RS232 Comimunicali on nai 2128s se sht ede dd eee 3 11 Selimi the LED Contrasts paa dre e i 3 12 Displaying Numerical Values in RADIX A 3 13 Setting the Shine Speed sc were hcg tag cess cv gn tg aces peed a eae 3 13 selecting the Displayed D ann 3 14 selecting the Operational Mode sic iso soon sedi n je sd rasen edin kand 3 15 Resetting System Default Parameters asi sdu Ee e s de 3 16 9 0 Preparing the Fluld Systemene te i eea e A e i o Ta ga a 3 18 Io G TE RODONIT d 3 19 Sample Collection Preparation and Measurement nc cc ece cecocececececese 4 1 AT Sample Preparation enet usd de be ket 4 1 YSI Model 1501 25 microliter Zanter 4 2 YSI Model 1502 Capillary Tube Injector cee aaaan ne anen eee e eee vene e vene v eee eee eee 4 3 Principles of Operation genin 5
45. Z wel i pe PROBE 0 TEMP 1 INJ SW 2 MORE 3 7 2 Select Temperature by pressing 1 then ENTER The display will show the current chamber temperature CHAMBER TEMP XX XX Press MENU to return to the Main menu 7 3 Checking the Sample Chamber Injection Switch Status Go to Main menu by pressing MENU until you see RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Diagnostics by pressing 3 then ENTER PROBE 0 TEMP 1 INJ SW 2 MORE 3 Select Injection Switch by pressing 2 then ENTER INJECTION SWITCH OFF Inserting an injection device will trigger the switch and the change in state will be indicated ON OFF on the display Press MENU to return to the Main menu 7 4 Turning the Stir Motor On and Off RUN 0 RECALL 1 SETUP 2 DIAG 3 Q ak ie E 5 5 5 S Lei io CH Lei Lei v 5 oa ti zZ C D E Z Lei ie Lei Ch CH Select Diagnostics by pressing 3 then ENTER PROBE 0 TEMP 1 INJ SW 2 MORE 3 7 3 9 STIR 0 PUMPS 1 PRINT 2 MORE 3 Lei CH Ka Ch O O CH S lt gr CH Lei Lei Ge i va LA et GI CH 5 ti Z wel ti ze Select Stir by pressing ENTER STIR OFF 0 ON 1 Turn the stir motor on or off by pressing the appropriate number key and then ENTER Press MENU to return to the Main menu 7 5 Turning Pump Motors On and Off RUN 0 RECALL 1 SETUP 2 DIAG 3 Q ct 5 si CH 5 S Lei io CH Lei Lei ae 5 oa ti
46. alibrate the instrument replace the Reference solution and repeat the calibration process UNSTABLE REFCUR PRESS MENU This fault can be generated in two different scenarios One during the calibration process the second during a sampling cycle 9 9 If it occurs during calibration it is because the measured reference current after the calibrator injection is different from the measured reference current after the linearity injection by more than 5 If it occurs during sampling it is because the measured reference current after the sample injection is different from the measured reference current after the original calibration injection by more than 20 POSSIBLE CAUSE Probe and membrane not stabilized as a result of recent power up or power interruption CORRECTIVE ACTION Enter PROBE Diagnostics and check to see if current is stable or falling Allow to stabilize then re calibrate SECTION 7 1 POSSIBLE CAUSE Supply tubing crimped prohibiting reference solution flow to the chamber CORRECTIVE ACTION Visually inspect all pump tubing 9 2 Possible Failure Conditions The following are possible failures that may go undetected unless confirmed visually but can affect instrument performance Air bubble in the sample chamber 9 10 POSSIBLE CAUSE Loose fittings on supply bottles CORRECTIVE ACTION Tighten fittings POSSIBLE CAUSE Supply line check valves stuck open CORRECTIVE ACTION Flush or replace check valves
47. amber as described in Section 8 3 8 2 Preventative Maintenance The YSI 1503 Preventative Maintenance Kit contains all the supplies necessary to keep your SPORT operating properly Perform all the maintenance procedures in Section 8 3 through 8 7 every 6 months or as required IMPORTANT The Waste bottles both reagent bottles the 5 00mL mixing bottle and the 3cc Syringe provided in the YSI 1504 Starter Kit should be cleaned every week with a disinfecting agent The bottles and Syringe should be rinsed completely with distilled water after cleaning to remove any disinfecting residue which would cause membrane failure if it got into the system 8 3 Cleaning the Sample Chamber 8 2 Clean the sample chamber every month Unscrew the probes from their housings If the membrane has passed the tests in Section 6 7 you don t have to replace it but you shouldn t let it dry out If the chamber is off the instrument for more than 15 minutes the membrane should be replaced Disconnect the supply and waste line tubing from the fittings on the sample chamber manifold Remove the two screws securing the injection block to the top of the sample chamber and move it to one side Remove the 2 screws securing the sample chamber to the case panel Remove the screw holding the manifold to the chamber Be careful not to lose the two O rings that seal the connection Replace them if necessary Be careful not to discard the small magnetic stir bar A
48. asma lactate value Hemolysis can cause disagreement between readings on the Model 1500 and some reference methods Cell metabolism will result in increased lactate concentrations in the specimen through glycolysis which will result in erroneously high assay values Glycolysis is particularly rapid in whole blood specimens and these should be analyzed within an hour of drawing Refrigeration at 4 C will retard glycolysis and extend the acceptable delay to four hours Whole blood specimens must not be frozen Some authorities recommend the use of chemical preservatives to minimize glycolysis Fluoride in concentrations of 7 5 mg ml of blood Boric acid in concentrations of 8 mg ml of blood Sodium iodoacetate if sodium iodoacetate is used as the antiglycolytic it must be free of iodide and iodine YSI has not evaluated the effects of these antiglycolytics ZN WARNING The YSI SPORT is not suitable for use with samples containing thymol or other phenol preservatives These are interfering substances Before taking a sample from a whole blood specimen any cells which may have settled out must be resuspended Invert the collection tube three times gently enough not to cause frothing just before sampling Samples which have settled for more than an hour particularly if they have been refrigerated should be placed on a blood rotator for five minutes to break up clumps of cells Air bubbles or froth in the sample drawn into the si
49. ata only and does not have the ability to acknowledge the reception of the data Please refer to Figure 3 6 Printer Placement for positioning details INFRARED DATA SIGNAL 3 07 MAXIMUM Nw HP 82240 PRIN Figure 3 6 Printer Placement The instrument has the capability to print the data while running the samples or you may print the results later selectively by date or sample identification number Refer to Section 6 3 Recalling Stored Results Printing the results as you run samples also allows you access to information that is only available during this cycle Along with the actual result you will also have a record of the temperature initial baseline current sample current and reference current The exact sample format is shown in Figure 3 7 Print Format 3 19 A similar printout is generated when calibrating and checking linearity We recommend that the printer be used in this manner whenever possible for two reasons One if there were a complete electrical failure or accidental re formatting of the memory it could be possible that the sample results in memory may be lost or irretrievable Two the additional information on the printout is extremely helpful in detecting performance trends should the need arise for troubleshooting SAMPLE TEMP 22 44 C BASCUR 1 28 nA SAMCUR 21 73 nA REFCURI 11 12nA 04 08 98 09 08 001 10 07 mmol L EE E HAE EE PH E Figure 3 7 Print Format The exact format for a calibratio
50. ation of the plasma lactate and the cell lactate will not have taken place yet and the plasma lactate concentration will be higher Conversely if the sampling was done much later the lactate concentrations will be much closer or equal In a non lysing system the blood cell walls create a barrier that the intracellular lactate can hide behind When a blood sample is injected into the sample chamber the extracellular lactate dilutes into the buffer solution immediately The intracellular lactate does not however due to the time required to diffuse through the cell walls Given sufficient time the lactate would diffuse through the cell walls and then dilute into the buffer solution allowing all of the the lactate to be measured This is not practical however as the rate at which this diffusion would take place would severely lengthen the duration of each measurement cycle Therefore the only lactate that can be measured on a SPORT in a non lysing system is extracellular What this means quite simply is that without a way of assuring that the cell walls have been removed when we do a sample measurement we can never expect to get a true TOTAL LACTATE reading on the SPORT This can be done by introducing certain chemicals into the buffer solution inside the sample chamber that effectively tear apart the cell walls of the blood sample immediately upon contact This allows both the intracellular and extracelleular lactate to dilute immediately into the
51. buffer solution and because preservatives now can access the contents of the cell any additional lactate production is prevented within the sample Another variable that plays a significant role in the lactate equation is how the sample is cared for prior to measurement If the sample is exposed to elevated temperatures before measurement it is quite likely that additional lactate production will continue within the unlysed blood cells This can add to the 12 1 12 2 inaccuracy of determining an athletes actual lactate level which can affect the appraisal of the athletes performance IMPORTANT YSI strongly recommends that you use the YSI 1515 Cell Lysing Agent Without total cell lysis breaking apart of cell walls results obtained on whole blood can vary significantly depending on individual hematocrit levels on whether unintended partial cell lysis takes place on temperature changes and the amount of time that elapses from the time of sample collection until the analysis is completed The presence of the lysing agent in the buffer solutions eliminates these problems and also allows the results to be compared directly to more traditional total blood lactate laboratory techniques Using the YSI 1515 Cell Lysing Agent If your sample measuring protocol requires the total lysis of blood cells or if you are going to preserve the samples with the YSI 2315 Preservative Tube Kit for later analysis another step is required in the reage
52. d completely out with either deionized or distilled water This will prevent any of the salts that are present in the buffer and reference solutions from crystallizing and blocking the fluid path This can be done quite simply by first emptying all of the fluid bottles and then rinsing and filling the supply bottles with distilled or deionized water The system can then be either cycled using the priming function available in RUN mode or it may be done through DIAGNOSTICS using the individual functions Storage of the unit for less than a week can be done with the buffer and reference fluids in place NOTE It is possible that after a period of storage depending on the tubing condition the tubing inside the pump may stick closed preventing fluid flow Depending on whether it happens to the waste or supply tubing the chamber may overflow or it simply may not fill correctly when attempting to run the unit for the first time Should either of these things happen refer to Section 9 Troubleshooting for instructions on how to correct the problem 8 7 9 Troubleshooting This section deals vvith identifying and correcting problems that interfere vvith the performance of the SPORT The fault messages that the instrument may display are listed then a brief explanation of the fault is given Follovving each fault is a list of possible causes vvith recommended corrective actions It is important that a systematic approach is taken to identifying
53. drying out as a result of no fluid in the sample chamber The acceptable ranges are 15 mmol 14 30 to 15 70 mmol 128 3 to 141 7 mg dL 30 mmol 28 50 to 31 50 mmol 256 5 to 283 5 mg dL If the result is not within these ranges replace the membrane allow time for it to break in and repeat the calibration process If the result is acceptable press MENU and the display will read SAMPLE 0 CAL 1 PRIME 2 You are now ready to measure samples Calibration Manual Mode In Manual Mode you should calibrate the SPORT on these occasions e Every day at the beginning of testing e Whenever you change the membrane or install new reagent fluids e Whenever you turn the instrument on automatically prompted e After every fifth sample e Whenever you change injection devices A 3 C temperature change since the last calibration automatically prompted Prepare buffer and solution and have the 5 mmol L 15 mmol L and 30 mmol L calibration standards provided in the 1504 Starter Kit at hand With the SPORT set up as described in Section 3 3 proceed as follows Load the Syringepet or injector with 5 mmol L standard AN WARNING If you calibrate with the Syringepet measure with the Syringepet If you calibrate with the injector measure with the injector Otherwise you may get incorrect values Press MENU until the following display appears RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Run by pressing ENTER SAMPLE 0 CAL 1 PRIME 2 S
54. e chamber overflow Supply pump tubing size 5 32 OD x 1 32 wall Waste pump tubing size 3 16 OD x 1 32 wall Upper Body Sat eee Roller Body Make sure tubing is not twisted inside body before assembly Figure 8 2 SPORT Pump Assembly 8 7 Injection Port Seal Replacement An injection port seal is included with your instrument It is used during transportation and long term storage Replace the plug and O ring on it every six months because they can deteriorate and allow chamber fluid leakage Extra O rings and plugs are supplied in the Preventative Maintenance Kit 8 8 Recharging the Battery A fully charged battery will provide a minimum of 8 hours operating time At this time or shortly thereafter a warning will be displayed after each sample cycle alerting you to a low battery condition To recharge the battery switch the power off plug the wall charging unit into an available AC outlet and connect the other end to the instrument interface panel at the Power Receptacle which is labeled CHARGE The charger is designed to fully charge the SPORT battery over a period of 8 hours disconnecting the charger prior to this will impact the amount of time available for battery only operation Once the battery is fully charged the red indicator lamp on the charger will flicker on and off rapidly This is the indication that the charger is floating An overnight charge should be more than adequate to insure a full day
55. elect the Calibration cycle by pressing 1 then ENTER VAIT 6 5 ERROR BASELINE PRESS MENU If you see this display press MENU then see Section 9 Troubleshooting If H INJECT 5mmol L STANDARD mt ez CH CH it n 5 io bei O S S est D CH echt Lei Wei ER lt 2 et CH Qa Inject the 5 mmol L standard When you remove the injection device you should see RUNNING STIR ON WASHING If the probe current is outside the expected limits you will see ERROR MEMB SENS If you see this display press MENU then see Section 9 Troubleshooting 9 WAIT INJECT 5 mmol CALIBRATOR CHECK ki Lag GI CH CH m Lei i io bei S 5 Pr D CH echt zy Lei Wei ER Lei pa CH gt Inject the 5 mmol L standard to verify a successful calibration Now you should see RUNNING STIR ON WASHING CAL CHECK XX XXmmol SAMPLE 0 CAL 1 PRIME 2 Q a gt CH O pe et eg CH 5 CH z lt ie Fh SY a gt CH 5 S S D CH o lt Z CH Q ga Co Lei 5 us CH ie CH eg CH e D CH lt N ENTER ID XXX H WAIT INJECT SAMPLE lt O Lei Q a 3 RUNNING STIR ON XXX XX XXmmol L WASHING E Z y X o 9 gd N O A oo 2 CH gt a n S el S g w 8 z 2 v e SCH Er E O E a S 8 SE z a R be S
56. f this does not help call YSI Technical Support for repair assistance Also at power up and prior to each sample cycle the processor does a fast self test of the electronics to see if any stored data has been corrupted or if the software has been physically changed If changes are detected warning messages will be displayed Refer to Section 9 Troubleshooting for a listing of the error messages and the possible causes and corrective actions Electrode Checks The background current and noise levels of the electrode are monitored prior to and during both the calibration and sampling cycles If the error limits are exceeded an appropriate error message will be displayed Refer to Section 9 Troubleshooting for a listing of the error messages and the possible causes and corrective actions 5 9 Temperature Error Checks The sample chamber temperature is checked at several different times during sampling to assure that the rate of change is not adversely affecting result reliability If the error limits are exceeded an appropriate error message will be displayed Refer to Section 9 Troubleshooting for a listing of the error messages and the possible causes and corrective actions Battery Level Checks The battery voltage is checked at several different times during sampling to alert you to conditions that may require reconnecting the battery charger The software will not allow continuation below certain levels as the stored data can be cor
57. ght Direct sunlight can cause rapid warming of the sample chamber warming the contents much faster than the larger fluid supply bottles If this happens it is quite possible that the 1500 SPORT will detect TEMPERATURE STABILITY ERRORS Simply keeping the unit in the shade regardless of ambient temperature will assure that the warming or cooling effects are consistent and therefore the results accurate Temperature Effect On Battery Life The length of available sampling time is affected by the ambient temperature while testing The colder the temperature the more battery life is affected Typically the results of our testing indicate the following estimated guidelines Ambient Temperature Sampling Duration Samples Ran 45 C 8 Hours 90 25 C 8 Hours 90 5 C 7 Hours 75 IMPORTANT 1 All testing done on units that were precharged for 8 hours using YSI Model 1550 wall mounted chargers 2 These tests were conducted running samples continuously 1 every 2 minutes It is quite clear that if fewer samples are being run the actual sampling duration will increase 3 Sampling duration is defined as starting point to software lockout Refer to Section 5 8 Software Structure Battery Level Checks for more information relating to battery voltage monitoring 15 1 15 2 16 Appendix G Accessory Producis The follovving products are available from YSI or your sales representative YSI Catalog Number 1501 1502 1503 1504
58. ght and allow several minutes for stabilization TEMP CHANGE FLUSHING This display will appear prior to any sample or calibrator injection if the sample chamber temperature has changed more than 1 C since the last wash cycle A full wash cycle will be automatically initiated to insure that the contents of the chamber are not artificially warmed because of direct sunlight or other phenomena Once the wash cycle is complete the chamber will be ready for injection Fluid System Faults ERROR REF CONCENTRATION LO This warning is generated when the Reference solution concentration is calculated to be less than 80 of the injected Calibration standard YSI 2327 5 mmol L Lactate Std It is possible that significantly high or low Reference solution concentrations can have a negative effect on the performance of the SPORT at the upper most part of the dynamic range If this fault is encountered during an attempt to calibrate the instrument replace the Reference solution and repeat the calibration process ERROR REF CONCENTRATION HI This warning is generated when the Reference solution concentration is calculated to be greater than 120 of the injected Calibration standard YSI 2327 5 mmol L Lactate Std It is possible that significantly high or low Reference solution concentrations can have a negative effect on the performance of the SPORT at the upper most part of the dynamic range If this fault is encountered during an attempt to c
59. hich bathes it In normal operation such changes are quite gradual and are corrected in the periodic recalibration of the instrument However concentrated detergent solutions may have a much more sudden effect Concentrated anionic detergents can cause readings to be abruptly elevated or depressed concentrated nonionic detergents generally depress readings For this reason concentrated detergent solutions should not be sampled No problem has been observed from the naturally occurring detergency of specimens Endogenous and Exogenous Substances YSI has tested hundreds of substances to determine whether they have any effect on the sensor system used in the SPORT YSI has also verified that the addition of Triton X 100 to the SPORT instrument buffer does not significantly alter the extent to which these materials interfere with the instrument The endogenous substances listed were all tested at levels far higher than can be found in the body and all were found to be noninterfering at the highest naturally occurring levels The column headed Interfering Level indicates the concentration at which each substance might be expected to cause an error of 1 mmol l in the lactate reading Certain exogenous substances can interfere with measurements and nothing should be added to the specimens except those anticoagulants and antiglycolytics recommended in Appendix B Sample Collection and Handling Recent information indicates that some of the ex
60. ied the waste bottle you are ready to prime the fluid system You must return to the Main menu RUN 0 RECALL 1 SETUP 2 DIAG 3 Press ENTER to get to the Run mode You will see this display SAMPLE 0 CAL 1 PRIME 2 Select PRIME by pressing 2 then ENTER You vvill see this display PRIMING REF ON STIR ON The instrument will run the reference fluid pump for approximately 30 seconds in order to fill the tubing lines Once the cycle has ended you will see this display NOTE The reference pump will only cycle if the selected mode is Automatic if Manual has been selected only the wash cycle will be called WASHING The instrument will run the buffer fluid pump for approximately 30 seconds in order to fill the tubing lines Once the cycle has ended you will see PRESS MENU Press MENU to return to the previous display you are now ready to calibrate and run samples on your instrument 3 8 Printer Option While there is not a printer built into the instrument there is a optional printer available either through YSI Model 1506 or your local Hewlett Packard dealer This printer HP Model 82240 is a battery powered unit controlled by an infrared signal that is generated from the SPORT The signal originates from the recessed interface panel located on the right hand side of the instrument It is important that the printer be positioned in such a way as to not interrupt this signal This is important because the SPORT sends d
61. ing All Sample Results Go to Main menu by pressing the MENU key until the display reads RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 Select Print by pressing 1 then ENTER PRINT ALL 0 BY DATE 1 ID 2 To print all results in memory press ENTER The display will be active during the printing process showing each result as it is printed It is not possible to halt the printing once it has started Once finished the display will return to the RECALL menu SAMPLES LCD 0 PRINT 1 XMIT 2 Use the MENU key to return to the Main menu 6 15 6 16 NOTE The printer must be placed in the correct position and turned on in order to receive the data Refer to Figure 3 6 for the printer placement if you are unsure Printing Today s Results Go to Main menu by pressing the MENU key until the display reads RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 Select Print by pressing 1 then ENTER PRINT ALL 0 BY DATE 1 ID 2 Select Date by pressing 1 then ENTER DATE MM DD YY XX XX XX NOTE The current date is what will appear on the display Press ENTER The display will be active during the printing process showing each result as it is printed It is not possible to halt the printing once it has started Once finished the display will return to the RECALL menu SAMPLES LCD 0 PRINT 1 XMIT 2 Use the MENU ke
62. ittsburgh PA e 15238 e Phone 800 395 5442 Cleaning Instructions IMPORTANT Before they can be serviced equipment exposed to biological radioactive or toxic materials must be cleaned and disinfected Biological contamination is presumed for any instrument probe or other device that has been used with body fluids or tissues or with wastewater Radioactive contamination is presumed for any instrument probe or other device that has been used near any radioactive source If an instrument probe or other part is returned or presented for service without a Cleaning Certificate and if in our opinion it represents a potential biological or radioactive hazard our service personnel reserve the right to withhold service until appropriate cleaning decontamination and certification has been completed We will contact the sender for instructions as to the disposition of the equipment Disposition costs will be the responsibility of the sender When service is required either at the user s facility or at YSI the following steps must be taken to insure the safety of our service personnel 1 In a manner appropriate to each device decontaminate all exposed surfaces including any containers 70 isopropyl alcohol or a solution of Va cup bleach to 1 gallon tap water are suitable for most disinfecting Instruments used with wastewater may be disinfected with 0 5 Lysol if this is more convenient to the user 2 The user shall take normal precauti
63. le chamber and reinstall stir bar SECTION 8 3 POSSIBLE CAUSE Stir bar not spinning CORRECTIVE ACTION Enter STIR diagnostics and check motor performance SECTION 7 4 ERROR OVER RANGE PRESS MENU 9 7 9 8 This fault is generated when the sample concentration exceeds the upper limit of the dynamic range 30 mmol No result will be stored in memory POSSIBLE CAUSE Sample concentration is too high resulting in high probe current CORRECTIVE ACTION Dilute sample 1 1 with the same buffer solution being used in the instrument and repeat SECTION 6 POSSIBLE CAUSE Excessive sample carryover on the outside of the Syringepet needle or capillary tube CORRECTIVE ACTION Repeat sampling procedure making sure to wipe excess sample from the outside of the injection device SECTION 6 Temperature Faults ERR BAD TEMP PRESS MENU The instrument will work only over the specified temperature range 5 45 C POSSIBLE CAUSE Ambient temperature too cold or hot CORRECTIVE ACTION Operate at ambient temperatures between 5 and 45 C TEMP UNSTABLE PRESS MENU POSSIBLE CAUSE If this fault appears after a sample cycle and no result has been displayed the sample chamber temperature is changing too rapidly more than 3 C from the sample cycle to the reference cycle CORRECTIVE ACTION Allow the instrument supply fluids to equilibrate at the ambient temperature CORRECTIVE ACTION Place the SPORT out of direct sunli
64. n infrared data link RS 232 capability to log data to a computer 2 2 Specifications Sample size 25 microliters Sample Medium Whole Blood Plasma Sampling Speed Manual Mode 60 seconds Auto Mode 96 seconds Detection range 0 to 30 mmol L 0 to 270 mg dL Resolution 0 01 mmol L 0 2 mg dL Calibration point 5 mmol L 45 mg dL Typical performance See Appendix A for typical performance data for whole blood and plasma Operating Temperature Range 5 to 45 C 10 to 90 RH non condensing Storage Temperature Range 0 to 60 C 10 to 90 RH non condensing Rechargeable at 95 to 130 VAC with the 115 volt charger or at 190 to 240 VAC with the 230 volt charger 47 to 63 Hz Battery Charger Output 13 8 VDC 300 milliamps 3 Instrument Setup We urge you to read through the entire Operations Manual before starting to use the SPORT Though the SPORT was designed for ease of operation prompting you through each operational step it is still helpful to know as much as possible about the instrument in order to get the most out of it 3 1 Unpacking Remove the instrument from its shipping container Be careful not to discard any parts or supplies Check off all items on the packing list and inspect all assemblies and components for damage If any parts are damaged or missing contact YSI Customer Service or your Dealer Representative immediately Do not discard the shipping container If servicing is required the
65. n concentrations of 1 mg ml of blood or boric acid in concentrations of 8 mg ml of blood will substantially halt glycolysis in most but not all specimens If sodium iodoacetate is used as an antiglycolytic it must be free of iodide and iodine ZN WARNING The YSI Model 1500 is not suitable for use with samples containing thymol or other phenol preservatives These are interfering substances ZN warning Frozen blood bank blood which contains a high percentage of glycerine is not a suitable specimen for diagnostic work with the Model 1500 Whole Blood If more than five minutes will elapse between the time you draw a specimen and analyze it you should mix the blood with an anticoagulant during or immediately after collection Analysis of whole blood without an anticoagulant is not recommended The preferred anticoagulants are EDTA in a concentration of 1 mg ml of blood or heparin sodium as commonly supplied in Vacutainer collection tubes Other effective anticoagulants which may be used are potassium oxalate in a concentration of 2 mg ml of blood sodium fluoride in a concentration of 5 10 mg ml of blood or sodium fluoride and EDTA in a concentration of 2 5mg fluoride and 1 mg EDTA per ml of blood These are not know to cause any error in the measurement of whole blood lactate but they may cause a reduction of the sample s hematocrit value with or without accompanying hemolysis This would lead to a slight error in the calculated pl
66. n extra one is supplied in your maintenance kit Clean the chamber with the appropriate disinfecting agent then flush it with copious amounts of warm water followed by a distilled water rinse Reassemble the chamber and manifold and attach the assembly to the case panel Reattach the injection block to the top of the sample chamber Remember to return the stir bar to the chamber Install new O rings or a new membrane if necessary and install the probes Extra O rings are supplied in your Preventative Maintenance Kit 8 4 Membrane Replacement To assure proper performance and prevent unexpected downtime we recommend that you replace lactate membranes every 14 days See Section 3 4 for membrane replacement procedure 8 5 Probe Cleaning With normal use enzyme sensors may become fouled and cease to operate in a normal mode A fouled sensor s output current will decrease and calibration may become unstable Since the severity of fouling will vary listed below are methods of cleaning which should be matched to the cleaning needs Follow the steps carefully and in order Sensor Maintenance It is necessary to maintenance the enzyme sensor when the 1503 PM kit is installed and periodically as needed 1 Remove the enzyme membrane and hold the probe with the electrodes facing up 2 Wad a small portion of a lint free tissue and wet it with 70 isopropyl alcohol 3 Using your thumb press the alcohol soaked wad against the probe
67. n or linearity printout provides the same relevant information but appears slightly different 4 Sample Collection Preparation and Measurement The SPORT is a versatile instrument that allows you to manipulate the sample in a number of different vvays e You can collect the blood sample using a lancette and capillary tube or by venapuncture into a tube or other collection device such as a vacutainer e The SPORT can be used with 2 injection devices The YSI 1501 25 microliter syringepet ideal for drawing samples from a blood collection tube and the YSI 1502 25 microliter capillary tube injector designed for injection directly from a YSI capillary tube e The sample can be prepared for immediate assay or for later analysis If you are going to preserve your sample for later analysis there are specific instructions that must be followed in order to insure the most accurate results Please refer to Appendix D Sample Preservation For more information on obtaining blood samples we refer you to two publications Approved Standard Procedures for the Collection of Diagnostic Blood Specimens by Skin Puncture edited by Jean M Slockbower National Committee for Clinical Laboratory Standards Volume 2 Number 5 pages 132 149 1982 Collection and Handling of Laboratory Specimens A Practical Guide edited by Jean Slockbower and Thomas A Blumenfield J B Lippincott Company Philadelphia 1983 ISBN 0 397 50520 5 4 1 Sample
68. nal use in sports medicine applications The SPORT is not intended for home use It is designed to provide quick measurement of lactate in whole blood or plasma Samples are injected with either a YSI 1501 blunt needle syringepet or a YSI 1502 capillary tube holder injector The SPORT automatically senses either of these injection devices and from that point it completes the sample analysis displaying the result and readying itself for any subsequent samples Each sample is stored in memory identified by date and time and also by athlete sample ID number if desired You can recall and display up to 150 sample values or you can transmit all of the stored data to a personal computer via the RS232 communication port An optional battery powered printer is also available to make hard copy records of the results Features Size 13 4 W x 14 8 L x 7 0 H inches 10 lb 33 0 x 27 9 x 17 8 cm 4 53 kg Water resistant splash proof Rechargeable 12 VDC battery w AC adapter Minimum of 8 hours of use between charges See Appendix F Temperature Effect on Operation Microprocessor control Alpha numeric liquid crystal display 16 character by 2 line with adjustable contrast Menu driven interface Automatic recognition of each sample injection Automatic calibration Athlete sample ID entry Sample result recall up to 150 stored in memory Complete diagnostic software Temperature compensation over a limited range Printer optio
69. nce Solution Current Sample Cycle p Net Reference Solution Currrent Calibration Cycle Unit Constant Sample Concentration Figure 5 5 Calibration Algorithms Calibrating the SPORT in Manual Mode with the 5 mmol L standard basically compares the relative concentration of the standard with the relative concentration of the unknown sample Calibrating in Automatic Mode compares the relationships of the calibration standard to the reference solution with the relationship of the unknown sample concentration to the reference solution this 5 7 relationship is then used in calculating the final concentration of the sample that is being measured Refer to Figure 5 5 for the algorithms used Z WARNING Running samples with an injection device other than the device used to calibrate the instrument may result in erroneous data 5 6 Linearity The sensor consists of an electrode and an enzyme membrane As the membrane ages it becomes underlinear or less sensitive see Figure 5 6 Linear Response Analyzer Ms Membrane Response ST Response A Calibration Substrate Concentration Figure 5 6 Aging Membrane Response You can check linearity with YSI 1530 30 mmol L standard after every calibration You can also check it at mid range with YSI 2328 15 mmol L standard Both standards should be injected as if it were a sample Both standards however can be measured as samples any time you want to check the li
70. nd oxalate may produce hemolysis which can cause disagreement between results from the SPORT and some reference methods ZN warning Frozen blood bank blood which contains a high percentage of glycerine is not a suitable specimen for diagnostic work with the SPORT Blood Serum Draw blood for serum preparation into a dry tube without additives Silicone coated tubes and stoppers lubricated with glycerine or silicone are acceptable After the specimen stands 15 minutes at room temperature loosen the clot from the wall of the tube with a sterile glass rod or applicator Add sterile beads if desired Centrifuge the specimen promptly to separate the serum from the clot If delays are unavoidable follow one these procedures 1 Refrigerate the whole blood at 4 C This is acceptable for periods of up to 4 hours Or prolonged storage isolate the serum from the clot within 30 minutes of collection and freeze it at 10 C or colder Serum will keep indefinitely at this temperature Thaw the frozen serum rapidly at 25 C and analyze promptly The freeze thaw cycle should not be repeated Tubes containing cells should never be frozen NOTE Freezing may precipitate aggluten or fibrinogen masses which may not redissolve You can remove these by centrifugation before assay Or by adding a chemical preservative Preservatives are not commonly used with serum however sodium fluoride in concentrations of 7 5 mg ml of blood EDTA i
71. ne packet YSI 2357 Buffer Concentrate One packet YSI 1515 Cell Lysing Agent One packet YSI 1515 Cell Lysing Agent H MIT ll BUFFER Figure 3 3 Reagent Preparation 3 5 3 4 Membrane Installation The starter kit contains a pack of four YSI 2329 lactate membranes Figure 3 4 Enzyme Membrane Installation To install a membrane first unscrew the probe retainer and pull out the probe Remove the membrane assembly that s on it You can use a toothpick or pointed tool to unseat the old membrane Be careful not to scratch the probe face Open one compartment of the plastic membrane pack and rinse the membrane inside with a few drops of salt solution YSI 2392 is provided in the 1504 starter kit Place one drop of salt solution on the probe face Then using the plastic membrane pack as an applicator press the O ring membrane assembly gently onto the probe face Figure 3 4 Be sure the membrane is seated on the probe face The top of the O ring will be flush with the shoulder Wipe excess salt solution from the probe body then return the probe to the sample chamber Finger tighten the probe retainer so that the O ring makes the necessary seal Return the membrane holder to the foil pouch and refrigerate it Note the expiration date on the membrane package We recommend that you keep a logbook in which the dates and lot numbers of reagents are recorded along with data from daily operational checks and other relevant information 3
72. nearity manually Acceptable Linearity Ranges For YSI 2328 Lactate Standard 14 30 to 15 70 mmol L 128 70 to 141 30 mg dL For YSI 1530 Lactate Standard 28 50 to 31 50 mmol L 256 50 to 283 50 mg dL For the very highest accuracy you should check linearity every hour of use 5 8 5 7 Temperature Compensation The sensitivity of the sensor varies with temperature changes The higher the temperature the more sensitive the sensor becomes A temperature compensating algorithm has been implemented to adjust for a maximum change of 3 degree Celsius within each sample cycle in auto mode Sample temp vs Reference temp or from the most recent calibration in manual mode If a greater change than this is occurring the rate of change is too fast to guarantee reliable results Error checking routines will display an error message if this is the case It is reasonable to expect that using the SPORT outside may result in some instances where the temperature rate of change rising and falling is quite fast Please refer to Appendix F Temperature Effect on Operation for further information about this subject 5 8 Software Structure The SPORT incorporates self test and error detection capabilities to prevent reporting erroneous results Microprocessor Self Test If at initial power up the display does not become active press the reset button If this does not correct the problem try turning the main power switch off and on again I
73. nents or parts in servicing or repairing the product or vi modification of the product in any way not expressly authorized by YSI THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY IN NO EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL INDIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY YSI Factory Service Centers United States ual YSI Incorporated e Repair Center e 1725 Brannum Lane e Yellow Springs OH e 45387 e USA Phone 937 767 7241 e Fax 937 767 9353 Europe Analytical Technologies e Lynchford House e Lynchford Lane e Farnborough Hampshire e GU14 GLT e England Phone 441 252 514711 e Fax 441 252 511855 YSI Authorized Service Centers California Fisher Scientific ISD e 2822 Walnut Avenue Suite E e Tustin CA e 92681 e Phone 800 395 5442 Georgia Fisher Scientific ISD e 2775 Horizon Ridge Court e Suwanee GA e 30174 e Phone 800 395 5442 Illinois Fisher e 1600 West Gleenlake Avenue e Itasca Ill e 60143 e Phone 800 395 5442 New Jersey Fisher Scientific ISD e 52 Fadem Road e Springfield NJ e 07081 e Phone 800 395 5442 18 2 Pennsylvania Fisher Scientific ISD e 585 Alpa Drive e P
74. nfecting agent Rinse with warm water AN WARNING Clean probes with isopropanol only and rinse with warm water Clean up all spills then reassemble 17 1 17 2 18 Appendix I Varranty and Shipping Information The YSI Model 1500 SPORT Analyzer is vvarranted for one year from date of purchase by the end user against defects in materials and vorkmanship exclusive of batteries Within the warranty period YSI will repair or replace at its sole discretion free of charge any product that YSI determines to be covered by this warranty To exercise this warranty write or call your local YSI representative or contact YSI Customer Service in Yellow Springs Ohio Send the product and proof of purchase transportation prepaid to the Authorized Service Center selected by YSI Repair or replacement will be made and the product returned transportation prepaid Repaired or replaced products are warranted for the balance of the original warranty period or at least 90 days from date of repair or replacement Limitation of Warranty This Warranty does not apply to any YSI product damage or failure caused by i failure to install operate or use the product in accordance with YSI s written instructions ii abuse or misuse of the product iii failure to maintain the product in accordance with YSI s written instructions or standard industry procedure iv any improper repairs to the product v use by you of defective or improper compo
75. ng once it has started Once finished the display will return to the RECALL menu SAMPLES LCD 0 PRINT 1 XMIT 2 Use the MENU key to return to the Main menu 6 5 Transmitting Results Over the RS232 Communication Link 6 18 NOTE Be sure all the communication parameters have been set correctly Refer to Section 3 Instrument Setup RUN 0 RECALL 1 SETUP 2 DIAG 3 Q et S Lem gt 5 5 z e 5 E Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 Select XMIT by pressing 2 then ENTER The display vvill be active during the transmitting process flashing each result as it is transmitted All stored data will be transmitted and it is not possible to halt the process once it has started Once finished the display vvill return to the RECALL menu SAMPLES LCD 0 PRINT 1 XMIT 2 Use the MENU key to return to the Main menu 6 6 Deleting Stored Results There are two ways to delete results that have been stored in memory The first method described edits out results no longer required This method requires that the result that is to be deleted be recalled on the display to make sure that the correct result is deleted The second method deletes all results Editing Results Go to the Main menu by pressing MENU until the display reads RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 To select viewing the results on the display press ENTER
76. nt preparation process explained in Section 3 3 Reagent Preparation Buffer Solution Fill the 500mL mixing bottle with reagent water Reagent water must be very pure Use high quality deionized water or distilled water Add one package of YSI 2357 Buffer Concentrate and stir waiting long enough to be certain that the buffer chemicals have completely dissolved Add one packet of YSI 1515 Cell Lysing Agent and stir Pour the solution into the instrument Buffer Bottle Reference Solution Fill the 500mL mixing bottle with reagent water Reagent water must be very pure Use high quality deionized water or distilled water Add one package of YSI 2357 Buffer Concentrate and stir waiting long enough to be certain that the buffer chemicals have completely dissolved Add 4 5 mL of YSI 1530 30 mmol L Calibration Standard and stir as necessary This should be measured out with the graduated syringe supplied in the 1504 Starter Kit Replace the bottle lid and shake the bottle for 15 seconds to insure complete mixing It is important for reliable results that the reference solution is completely mixed Add one packet of YSI 1515 Cell Lysing Agent and stir Pour this solution into the Reference Bottle PN WARNING If you switch from running with to running without the lysing agent or vice versa you cannot get reliable results unless you allow sufficient time for complete removal of the agent from the fluid system 12 3 12 4 13
77. of testing Whenever you change the membrane or install new reagent fluids Whenever you turn the instrument on automatically prompted Whenever you change injection devices It is recommended that a sample of the 5 mmol calibration standard should be ran at least once every two hours while testing in order to check calibration status If the result is outside the specified range then recalibration is necessary Prepare buffer and reference solutions and have the 5 mmol L 15 mmol L and 30 mmol L calibration standards provided in the 1504 Starter Kit at hand With the SPORT set up as described in Section 3 3 proceed as follows Load the Syringepet or injector with 5 mmol L standard AN WARNING If you calibrate with the Syringepet measure with the Syringepet If you calibrate with the injector measure with the injector Otherwise you may get incorrect values RUN 0 RECALL 1 SETUP 2 DIAG 3 as CH Lei Lei ti zZ C pk Z Lens D CH eh O 5 oa on Lei Wei E Lei ke POH CH E Lei Select Run by pressing ENTER SAMPLE 0 CAL 1 PRIME 2 Select the Calibration cycle by pressing 1 then ENTER WAIT ERROR BASELINE PRESS MENU Fb vet Cc Lei CH CH et Es z Lei O 2 Lei ko O D Lei DT S CH Lei Lei ii Z c GI CH 5 Co Ch CH Lei Ch Q T O 5 NO c S CH n S O jm ya H 3 INJECT 5mmol L STANDARD ki
78. ogenous substances listed are now drugs that are abused that is ingested at levels much higher than the usually recommended therapeutic doses In patients with higher than therapeutic levels there is hazard of gross masking of lactate concentration by the interfering substance The SPORT should not be used to analyze specimens containing any of these substances at or above the listed Interfering Level Physical Damage Never inject concentrated mineral acids concentrated bases or strong organic solvents into the YSI SPORT as these may permanently damage the enzyme membranes or the plastic parts of the probe and sample chamber Do not inject water insoluble oils or greases because it may be difficult to clear them from the sample chamber Blood specimens with excessive content of fats are not a problem Substance Weight Lactate Interfering Level mg dL mmol L Anticoagulants Sodium Oxalate 134 00 Heparin Sodium Dipotassium EDTA 404 46 Sodium Citrate 294 10 Preservatives Sodium Flouride 42 00 Iodoacetic Acid 185 96 24 346 1309 2 Iodoacetamide 185 95 3 356 180 Sodium Tetraborate 10 H2O 381 37 54 000 1416 Benzalkonium Chloride 396 11 1 200 30 Cetylpyridinium Chloride 357 99 Sodium Azide 65 01 588 90 Methylparaben 152 15 168 11 Phenol 94 11 6 7 0 71 Thymol 150 22 600 40 14 3 Substance Weight Lactate Interfering Level mg dL mmol L Lactic Acid Analogues DL a Glycerophosphate
79. ollecting the sample or you may collect the sample first then load the tube AN WARNING Precision and accuracy depend on sample loading technique Any air in the sample column will cause incorrect results Plunger Tube clamp Ps Tube Clamp Window Black band e Double white band YSI1505 Capillary Tube Figure 4 2 1502 Capillary Tube Injector The end of the tube with the double white band goes into the injector first The collar around the front part of the injector barrel is the tube clamp Each time you insert or withdraw a capillary tube you loosen or tighten the tube clamp so that the capillary tube can be inserted or withdrawn easily without sticking Just a small adjustment of the tube clamp one way or the other is necessary Slide the tube all the way in until it stops The tube should be sticking out about 1 2 an inch 12 mm sample will be visible in the section of the tube that is protruding and the black band should be visible in the tube clamp window 4 4 When you adjust the clamp it is not necessary to unscrew it completely Never should more than one full turn be necessary AN WARNING Any sample carryover on the outside of the tube could affect the measurement You should wipe off the tube or Syringepet needle with a sterile wipe like a Kimwipe Hold the injector directly above the injection port and slide it into the port until you feel it stop In one motion press the plunger all the way d
80. ollowing sample cycle is illustrated as if the reference solution were indeed being used If you are operating in Manual mode ignore the messages relative to the Reference solution Press the MENU key until the Main menu appears RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Run by pressing ENTER SAMPLE 0 CAL 1 PRIME 2 Select the Sample cycle by pressing ENTER The memory is limited to 150 results You can continue to run samples while the memory is full but the results vvill not be saved Stored results vvill have to be deleted in order to make room available The procedure for deleting stored results is described later in this section If the memory is full the display vvill read MEMORY FULL STOP 0 PROCEED 1 Selecting Proceed will allow you to run the sample but remember that the result will not be stored If you continue the display will read ENTER ID XXX When the ID display appears enter the ID as a three digit number example one is entered as 001 two hundred and thirteen as 213 then press ENTER and WAIT ct E n QO Lei is 2 fas lt lt CO us 5 Oo o e If the probe current is not vvithin the expected range you will see this display ERROR BASELINE PRESS MENU If you see this display press MENU then see Section 9 Troubleshooting INJECT SAMPLE mi D CH CH Ei Lei i gr Ki S S ct D CH echt ag Lei se S Lei C
81. om the decimal fraction If you turn on the RADIX mode a comma will appear instead of a period Go to Main menu by pressing MENU until you see RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 Select MORE by pressing 3 then ENTER LCD 0 RADIX 1 STIR 2 MORE 3 Select the RADIX mode by pressing 1 then ENTER RADIX PERIOD 0 COMMA 1 Turn the RADIX mode on by pressing 1 then ENTER Press MENU until the Main menu reappears Setting the Stirring Speed The stirring speed can be adjusted to assure consistent stirring When making this adjustment speed up the stirring until the stir bar tumbles inside the sample chamber Once you have done this slowly reduce the stirring speed until the stir bar settles into a uniform spinning motion Go to Main menu by pressing MENU until you see 3 13 RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 H LCD 0 RADIX 1 STIR 2 MORE 3 N CH GH Ch Q E O Ki CH o Lei ke CH Lei Lei 4 gq LA zb D CH 5 ti Z m a Select Stir by pressing 2 then ENTER STIR SPEED UP 0 DN 1 Pressing the ENTER key will speed up the stirring by a preset increment Pressing 1 then ENTER will slow down the stirring by a preset increment Press MENU until the Main menu reappears Selecting the Displayed Units RUN 0 RECALL 1 SETUP 2 DIAG 3 Q ot D
82. on It has been type tested and found to comply with the limits for a Class A or Class B computing device in accordance with the specification in Subpart J of Part 15 of FCC Rules which are designed to provide reasonable protection against such interference in a residential installation However there is no guarantee that interference will not occur in a particular installation If this equipment does cause interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e reorient the receiving antenna e relocate the computer with respect to the receiver e move the computer away from the receiver e plug the computer into a different outlet so that the computer and receiver are on different branch circuits If necessary the user should consult the dealer or an experienced radio television technician for additional suggestions The user may find the following booklet prepared by the Federal Communications Commission helpful How to Identify and Resolve Radio TV Interference Problems This booklet is available from the U S Government Printing Office Washington D C 20402 Stock No 0004 000 00345 4 19 1 IVD Y SI Life Sciences sw YSI Incorporated 1725 Brannum Lane Yellovv Springs Ohio 45387 USA 937 767 7241 e 800 659 8895 Fax 937 767 8058 Info ysi com www YSI com Er CE
83. ons to prevent radioactive contamination and must use appropriate decontamination procedures should exposure occur 3 If exposure has occurred the customer must certify that decontamination has been accomplished and that no radioactivity is detectable by survey equipment 4 Any product being returned to the YSI Repair Center should be packed securely to prevent damage 5 Cleaning must be completed and certified on any product before returning it to YSI Packing Instructions 1 2 Clean and decontaminate items to insure the safety of the handler Complete and include the Cleaning Certificate Place the product in a plastic bag to keep out dirt and packing material Use a large carton preferably the original and surround the product completely vvith packing material Insure for the replacement value of the product Cleaning Certificate Organization Department Address City State Country Phone Model No of Device Lot Number Contaminant if known Cleaning Agent s used Radioactive Decontamination Certified Answer only if there has been radioactive exposure Yes No Cleaning Certified By 18 4 19 Appendix J Required Notice The Federal Communications Commission defines this product as a computing device and requires the following notice This equipment generates and uses radio frequency energy and if not installed and used properly may cause interference to radio and television recepti
84. ort 3 6 System Setup You use menus to set up the SPORT system Once set up the system parameters are maintained in memory until they are changed purposely IMPORTANT The SPORT allows you to go through each menu step by step by selecting options on the display Each menu has a number of options and each option is identified by a number Pressing the number that corresponds to the desired option moves the display cursor to that option However the selection will not be made until you press ENTER Whenever a new menu is displayed the cursor defaults to the option identified by the digit 0 Refer to Figure 3 5 for the keypad layout and cursor location 3 7 3 8 Figure 3 5 SPORT Keypad How the five function keys and the numeric keypad are used ON ON is used as a user acknowledgement of a low battery condition ENTER ENTER commits the data you ve keyed in with the numeric keypad It does this from any menu at any level MENU Each time you press MENU you back up one level to the previous menu CANCEL CANCEL stops the current operation starts a wash cycle and brings up the Main menu DELETE DELETE is active only when the display is showing recalled sample values It is used to delete the value showing at the moment NUMERIC KEYPAD The keys numbered from 0 to 9 allow you to enter time date ID numbers menu selections and other data The following sections in this chapter show you how to set up
85. own with your thumb Maintaining pressure on the plunger after it has bottomed out remove the injector from the port Release the tube clamp and slide the used tube out of the injector S WARNING The used capillary tube is a biohazard and should be disposed of in a manner consistent with safety and the laws in your area 5 Principles of Operation 5 1 Sensor Technology The sensor technology of the YSI SPORT is based on the principles conceived by Dr Leland Clark formerly of Children s Hospital Foundation Cincinnati Ohio The immobilized enzyme membrane was invented by YSI and is covered by U S Patent 4 073 713 This sensor technology has been used successfully since 1975 in the YSI 23A Blood Glucose Analyzer then later in the YSI 27 Industrial Analyzer the YSI 23L Blood Lactate Analyzer the YSI 2300 STAT Glucose L Lactate Analyzer and the YSI 2700 SELECT Chemistry Analyzer REACTION 2 SILVER PLATINUM es a eg Be s Q ACETATE MEMBRANE E OXIDASE SUBSTRATE on MEMBRANE Figure 5 1 Sensor Probe and Enzyme Membrane The probe is fitted with a three layer membrane containing immobilized lactate oxidase in the middle layer Figure 5 1 shows an exploded view of the membrane and its relationship to the face of the probe The face of the probe covered by the membrane is situated in a buffer filled sample chamber into which a sample is injected Some of the substrate diffuses through the membrane When it
86. pper can lead to significant errors in the form of low lactate readings Because it is difficult to detect bubbles visually in whole blood specimens you should exercise great care Any unexpectedly low lactate readings may indicate a bubble if possible repeat the determination ZN warning Frozen blood bank blood which contains a high percentage of glycerine is not a suitable specimen for diagnostic work with the SPORT 11 4 12 Appendix C Cell Lysing Obtaining reliable results that have value requires a basic understanding of what is going on inside the whole blood sample at least as it relates to lactate Lactate is always present in the blood plasma at some level due to minor anaerobic activity As the consumption of free oxygen increases during exercise anaerobic activity increases and so does the lactate concentration Lactate production starts inside the muscle cell and the lactate then diffuses through the cell wall into the blood cell carrier fluid plasma The plasma lactate will then in turn diffuse through the red blood cell walls in order to achieve an equilibrated state It takes several minutes for this to happen As a result depending at which point in an exercise the whole blood sample is taken the concentration of plasma lactate extracellular may be significantly different than the concentration of lactate in the red blood cell intracellular For example if the sampling was done very early the equilibr
87. ra zines and hydrazides hydroxylamines oximes and a few other compounds of molecular weight below 150 which are cationic or uncharged in neutral solution can interfere Homologues and isomers may be expected to behave similarly except that relative response generally declines with increasing molecular or ionic bulkiness Hydrogen Sulfide Hydrazine Methylhydrazine Phenylhydrazine Oxamic Hydrazide Hydroxyet hylhydrazine Acetone Oxime Hydroxylamine and Sodium Borohydride are also known to give significant relative response Relative response to reducing agents may vary from membrane lot to membrane lot and may depend on the service history of the probe and membrane 14 1 14 2 The follovving reducing agents may result in an elevated background current when present at low levels only a few milligrams per liter The Aniline Phenol Thiocarboyhdrazide Thiourea Resorcinol Guaiacol 2 Mercaptoethanol Ethyl Carbazate Formic Acid Hydrazide Catechol Acetaminophen following materials may cause trouble at higher concentrations 2 Amino 4 nitrophenol Hydroquinone p Cresol p Phenylenediame Isoniazid o Toluidine Methimazole 2 Hydroxybenzyl Alcohol 4 Ethylphenol p Aminophenol Pyrogallol Oxalyl Dihydrazide N N Dimethylhydroxylamine Sodium Azide Calibration Shift by Detergents The sensitivity of the platinum anode of the sensor is affected somewhat by absorption or desorption of material from the buffer w
88. rared Printer Port is used to transmit data to the optional battery powered printer The Power Receptacle is a DC power inlet The battery charger supplied with the SPORT is connected here to recharge the instrument battery IMPORTANT Probe polarization will be disrupted when power is turned off Time for restabilization will be needed when power is restored 3 3 Reagent Preparation If you are going to use only the manual mode of operation the only solution you will need to prepare is the Buffer solution as the Reference solution is only used as part of the Automatic mode You can prepare the reagent solutions for the SPORT yourself These reagents along with a mixing bottle and a graduated syringe 3cc are provided in the YSI Model 1504 Starter Kit which must be ordered separately Please note that the reagents in the starter kit may also be ordered separately IMPORTANT The mixing bottle and Syringe must be cleaned every week See Section 8 2 Preventative Maintenance If your sample measuring protocol requires the total lysis of blood cells or if you are going to preserve the samples in YSI 2372 Preservative Tubes for later analysis you must refer to Appendix D Sample Preservation for further information before proceeding Automatic Mode Buffer Solution Fill the 500mL mixing bottle with reagent water Reagent water must be very pure Use high quality deionized or distilled water Add one package of YSI 2357 Buffer Concent
89. rate and stir waiting long enough to be certain that the buffer chemicals have completely dissolved Pour buffer solution into the instrument Buffer Bottle Put the cap back on the Buffer bottle and place it back into the instrument Reference Solution Prepare 500 mL of Buffer solution pour it into the Reference Bottle and add to it4 5 cc of YSI 1530 30 mmol L Calibration Standard This should be measured out with the graduated syringe supplied in the 1504 Starter Kit Replace the bottle lid and shake the bottle for 15 seconds to insure complete mixing It is important for reliable results that the reference solution is completely mixed Rinse the mixing bottle with deionized or distilled water IMPORTANT Screw the bottle lids on tightly before putting them back into the instrument If you don t fluid may leak out when you pick up the SPORT by its handle Manual Mode Buffer Solution Fill the 500mL mixing bottle with reagent water Reagent water must be very pure Use high quality deionized or distilled water Add one package of YSI 2357 Buffer Concentrate and stir waiting long enough to be certain that the buffer chemicals have completely dissolved Pour the buffer solution into the instrument Buffer Bottle The remaining fluid may be saved inside the mixing bottle until it is needed later Reagent Preparation 500 mL Reagent Water One packet YSI 2357 Buffer Concentrate 500 mL Reagent Water 4 5 cc YSI 1530 Lactate Standard O
90. rupted if the battery gets to very low levels Warnings will be displayed at 12 1 volts and a software lockout will occur at 12 0 volts Once the software lockout is enabled the battery charger must be connected This lockout will remain in effect until the battery is recharged to 12 8 volts A separate electrical hardware shut off will occur at 11 5 volts to prevent any accidental complete discharging of the battery if the SPORT sits unattended for long periods of time For more information refer to Section 8 8 Recharging the Battery and Section 9 Troubleshooting for a complete description of the warning messages and corrective actions 1500 SPORT Menu Flow Chart Main Menu SETUP 2 DIAG 3 JE JI Run Mode Recall Mode Setup Mode Diagnostic Mode H Probe Current Run Ref Cycle Ka RECALL 1 Date SES Sample Display Time ID H View Results RS232 Calibrate Run Wash Cycle All Date Cal Check ID Delete Results Baud Rate Chamber Temp Data Bits Injection Switch Prime Pumps Stop Bits Stir Motor All Individually Parity Pumps Print Results LCD Contrast Reference Pump Radix Mode Buffer Pump Stir Speed Print Xmit Results Units Measure Infrared Link RS232 Link perational Mode Keypad mg dL mmol L Auto Display Manual Version System Default Battery Voltage
91. s The SPORT does not sense fluid levels automatically so you have to look to see that you have enough If you run out and don t realize it you ll get wrong data and you may lose samples Replace the Buffer and Reference solutions if they are low or have been in the instrument longer than a week Follow the instructions in Section 3 3 Reagent Preparation After a solution change prime the fluid system Select RUN O from the Main menu Selecting PRIME 2 will run predetermined amounts of reference and buffer solutions in order to fill all the supply lines completely NOTE There is usually air in the waste tubing This does not affect performance Check for Air Bubbles in Chamber Examine the sample chamber for any air bubbles that may have stuck to the membrane and or stir bar Bubbles that do not flush out of the chamber can give you artificially high lactate readings Refer to Section 9 Troubleshooting for directions in removing air bubbles 8 1 Check for Leaks Examine the tubing for leaks and check the sample chamber for large air bubbles which may be caused by either loose connections or worn pump tubing Refer to Section 8 6 Tubing Replacement if the tubing needs to be replaced Clean up spills Spills should be cleaned up immediately to prevent corrosion and biohazard buildup Blood spills must be carefully and safely removed If there is any evidence of salt build up around the sample chamber disassemble and clean the ch
92. s it into the Waste Bottle The Reference Pump is identical in operation to the Buffer Pump It is used to pump the reference solution The Sample Chamber is made of clear acrylic plastic White and black probe holders are attached to the sides The enzyme probe is in the white holder The membrane is mounted on an O ring that serves as a fluid seal between the chamber and the probe The temperature probe is in the black holder A small black O ring on the tip of the temperature probe serves as a fluid seal The Injection Port is on the top of the Sample Chamber Inside the port is a sensor that detects the insertion of a YSI injection device A removable cap is threaded into the top of the Injection Port This cap must be removed and replaced with the supplied threaded plug before moving or storing the instrument The Keypad is a 15 key membrane switch with numeric keys from 0 to 9 and 5 function keys ENTER MENU CANCEL DELETE and ON The Display is a 2 line by 16 character liquid crystal display Figure 3 2 SPORT Interface Panel The following switches and connectors are located in a recessed area on the side of the instrument The Reset Switch is used for a hard reset of the operating system 3 3 The Remote Communication Port is a 25 pin RS232C serial port It is used to interface vvith personal computers The Power Switch is a rocker switch that allows you to control power to the instrument The Inf
93. s message will be displayed if after being prompted to inject either a calibrator or a sample 5 minutes passes Pressing MENU will move you to the previous menu from where you will be allowed to continue once more 9 3 Battery Faults LOW BATTERY PRESS ON A check of the battery voltage is done during the sample cycle At 12 1 volts this message is displayed Sampling will not be prohibited at this point typically there is enough battery reserve at this point to continue for at least 1 hour of continuous testing minimum of 40 samples Press the ON key to clear the message and allow operation of the instrument CONNECT CHARGER PRESS ON This message is displayed when the level drops below 12 0 volts At this point in order to continue you must connect the battery charger The software will not allow you to continue sampling though until the battery has been recharged to a level of 12 8 volts This is done to protect the discharge recharge capacity of the battery and insure the maximum amount of good usable battery life Enzyme Sensor Faults ERROR BASELINE PRESS MENU This fault is generated because the probe baseline current is above the allowable starting point of 6 nanoamps It is common to see this fault if you try to run samples too soon after turning the instrument on or after putting on a new membrane Remember that new membranes need time to break in POSSIBLE CAUSE Reference solution in sample chamber CORRECTIVE ACT
94. supply bottle cap to verify clear blockage or replace vent POSSIBLE CAUSE Sample chamber inlet blocked CORRECTIVE ACTION Disassemble chamber check for blockage in inlet port lower SECTION 8 3 Sample chamber overflows through the injection port POSSIBLE CAUSE Waste tubing line in pumps crimped or stuck shut CORRECTIVE ACTION Disassemble pumps and open tubing or replace if more than 6 months since last replaced SECTION 8 6 POSSIBLE CAUSE Waste line blocked CORRECTIVE ACTION Clear tubing or replace SECTION 8 6 POSSIBLE CAUSE Waste line check valve stuck open CORRECTIVE ACTION Flush check valve or replace POSSIBLE CAUSE Bottle vent plugged causing positive pressure in the waste bottle CORRECTIVE ACTION Loosen waste bottle cap to verify clear blockage or replace vent POSSIBLE CAUSE Capillary tube plug broken loose from tube and stuck in waste chamber port CORRECTIVE ACTION Disassemble chamber check for blockage in outlet port upper SECTION 8 3 POSSIBLE CAUSE Sample chamber outlet blocked CORRECTIVE ACTION Disassemble chamber check for blockage in outlet tube due to salt buildup and clean if necessary SECTION 8 3 Stir bar fails to spin 9 12 POSSIBLE CAUSE Stir bar stuck to chamber CORRECTIVE ACTION Clean the sample chamber SECTION 8 3 POSSIBLE CAUSE Stir motor failure CORRECTIVE ACTION Go to stir motor diagnostics and verify SECTION 7 4 9 13 9 1
95. t recall at any point by pressing MENU Recalling a Result From Any Previous Date Go to the Main menu RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 To select viewing the results on the display press ENTER DISPLAY 0 DELETE ALL 1 To select viewing the results on the display press ENTER LIST ALL 0 BY DATE 1 ID 2 9 DATE MM DD YY XX XX XX Lei CH CH el E J CO CH o lt ke CH n n pm i 09 D CH 5 ti Z wel ti SS NOTE Today s date will appear Key in a date as described in Section 3 6 System Setup then press ENTER Example January 17 2003 is entered as 01 17 03 ENTER The first sample result stored in memory on that date will be displayed MM DD YY HH MM XXX X XXmmol L Every time you press ENTER the next result stored will be displayed You can cycle step by step through all of the results on this date The ID number will change as necessary to match each sample When all of the results have been displayed you will see this display NO SAMPLES PRESS MENU NOTE It is possible to end the result recall at any point by pressing MENU 6 13 6 14 Recalling a Result By ID Number Go to Main menu by pressing the MENU key until the display reads RUN 0 RECALL 1 SETUP 2 DIAG 3 5 Lei CH Ch QO et Gei CH QO poo o Lei io CH Lei Lei he 5 oa a E CH 5 tH Z wei ti
96. terfere with the SPORT sensor system resulting in erroneous readings See Appendix E Effects Of Selected Substances 5 2 Fluid System INJECTION DEVIC SS Ne SH SAMPLE CHAMBER S1 Enzyme Probe MI Reference Pump Motor S2 Temperature Probe Me Buffer Pump Motor S3 Injection Switch SBI Magnetic Stir Bar Figure 5 2 SPORT Fluid System 5 3 5 3 Measurement Methodology The algorithm used to calculate results allows the SPORT to process samples as quickly as possible while maintaining high accuracy and reliability The result is calculated from a group of four readings depending on the chosen operational mode e Initial baseline current e Sample Calibrator plateau current e Reference plateau current Auto mode only e Calibrator concentration value current For initial calibration 25 microliters of calibration standard is dispensed into the chamber which already contains approximately 500 microliters of buffer The sensor response increases until it reaches a plateau approximately 30 seconds The reference pump then flushes the chamber for approximately 33 seconds and the sensor response changes to a different value At this point the instrument calculates the relationship of the known standard to the reference solution and stores this information until another calibration injection is performed The
97. th a record of daily operational checks and other relevant information Membrane Integrity Test Use YSI 2363 Potassium Ferrocyanide FCN to determine if your membranes are intact This solution is packaged in your starter kit and may also be ordered separately With the instrument in SAMPLE mode inject 25 microliters of the solution with whichever injection device was used for calibration The reading should be below 0 3 mmol L 2 7 mg dL If it is greater repeat the test If it s still too high change the membrane Linearity Test Use YSI 1530 30 mmol L or YSI 2328 15 mmol L Lactate Standard to check the linearity of the sensor These standards are packaged in your starter kit and also may be ordered separately Acceptable Ranges YSI 2328 14 30 to 15 70 mmol L 128 3 to 141 7 mg dL YSI 1530 28 50 to 31 50 mmol L 256 5 to 283 5 mg dL 6 21 6 22 7 Instrument Diagnostics Diagnostic routines in the software provide troubleshooting capability not only in manufacturing but also in field service and user maintenance These routines check the following elements of the electronic and fluid systems e Probe current Buffer and reference fluid cycles are independently accessible to monitor probe current change e Sample chamber temperature e Injection switch status e Motors The pump motors and stir motor can be run separately or simultaneously e RS232 A test data string is sent over the serial communications link
98. the instrument The correct display for each step is shown If the display does not respond as shown press MENU and try again Instructions are given starting each time from the Main menu As you become more familiar with the menu structure this will not be necessary Date Go to Main menu by pressing MENU until you see RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 Select Date setup by pressing ENTER DATE MM DD YY XXIXXIXX DAY The first and second digits you press define the desired month 01 is January and 12 is December The third and fourth digits you press define the date you can enter any pair of digits from 01 to 31 The fifth and sixth digits define the last two digits of the desired year When you see the date you want on the screen press ENTER Example January 15 2003 is entered as 01 15 03 ENTER DATE MM DD YY 01 15 03 WED If you have entered an impossible date January 99 2003 for example the display will read DATE PRESS ENTER 01 99 03 Press ENTER to get back to the previous menu for another attempt to set the date If the date you have entered is acceptable press MENU until you return to the Main menu 3 9 Time The SPORT incorporates a 24 hour clock Go to Main menu by pressing MENU until you see RUN 0 RECALL 1 SETUP 2 DIAG 3 Select Setup by pressing 2 then ENTER DATE 0 TIME 1 RS232 2 MORE 3 Select Time by pressing 1 then
99. ting Calibration standards into the instrument This will allow you to develop the necessary technique while also letting you visually assess how well you are doing by checking the result on the display Depress the plunger all the way with your thumb Lower the Syringepet needle into the sample then slowly using light thumb pressure let the plunger come all the way back Examine the glass barrel to see if there are any air bubbles in the sample If there are any dispense the sample back into the container you drew it from and try again If there are no air bubbles present you are ready to inject the sample into the instrument Once you are sure the sample is ready for injection hold the Syringepet directly above the injection port and slide the needle in until you feel it stop Press in the plunger all the way with your thumb Keeping thumb pressure on the plunger remove the Syringepet from the port AN WARNING Any sample carryover on the outside of the needle could affect the measurement You should wipe off the tube or Syringe pet needle with a sterile wipe like a Kimwipe prior to each sample injection YSI Model 1502 Capillary Tube Injector YSI 1505 capillary tubes are provided in the Starter Kit These tubes which can be ordered separately are preset to a volume of 25 microliters There is a double white band on the tube opposite the end that you put into the sample You may load the capillary tube into the injector before c
100. ty run as a sample Injection prompted measure baseline current Inject 25 microliters of linearity standard Measure sample linearity check current and deduct baseline current Measure reference solution current and deduct baseline current Calculate sample linearity check to reference solution ratio Divide by original calibrator to reference ratio multiply by unit constant 5 Display result and flush chamber with fresh buffer Ready for next sample Figure 5 3 Automatic Mode Probe Current Profile 5 5 MANUAL CAL MODE CHECK LINEARITY SUBSTRATE CONCENTRATION 15 TIME Injection prompted measure baseline current Inject 25 microliters of calibration standard Measure calibrator current deduct baseline and save value Flush chamber with fresh buffer solution 5 minute window allowed for cal check injection Injection prompted measure baseline current Inject 25 microliters of calibration standard Measure sample calibrator current and deduct baseline current Flush chamber with fresh buffer solution Caleulate sample to calibrator ratio and display result No fixed requirement for liearity check run as a sample Injection prompted measure baseline current Inject 25 microliters of linearity standard Measure sample linearity check current and deduct baseline current Calculate sample to calibrator ratio and display result Flush chamber with fresh buffer solution Ready for next sample Figure 5 4
101. ua aso cc co oeceee 6 18 GG Deleting SOTE ERES Sip e eines ale ees 6 19 PUT ET 6 19 Deleting All ResultSet do de da 6 20 BS Daily Operalon AC Beek sy ices dat kd eti 6 21 Instrument Diagnostics E 7 1 71 Checking Probe Curr nti cessed sh 7 1 7 2 Checking Sample Chamber Temperature sesiuiissocciosn sis Skadon d dreri dron 7 2 7 3 Checking the Sample Chamber Injection Switch Stage 7 3 7 4 Turning the Stir Motor On and OI gadi r dan dk 7 3 19 Turning Pump Motors On and QIT na csesonnassci sh red d sin resh reth dos 7 4 7 6 Sending a Test Print String Over the Infrared Data Lin Ko aauuaua aaa ann aa nana an nn en ne even ce eee eoee 7 5 7 1 Sending a Test Data String Over the RS232 Data Lmk aaa na nana nne vr ee vereni 7 5 KE shte Tie Keypads es e i nd e 7 6 7 97 Testing then TE 7 1 10 11 12 13 14 7 10 Checking the Software Version Number 7 8 7 11 Checking the Battery Voltage iacizesoscca kas s ironin ae 7 8 Maintenance dini so ne eee dd n dd ua e u 8 1 L Daly Main DANCE vra ege e e aa EATS 8 1 Empty th Waste Bottles saa shend coadee us sdtaanscnanesoeeummaaenacceahes 8 1 Check Buffer and Reference Bottle Levels Geteste eet edu ege ease 8 1 Check for Air Bubbles in Chamber ksi nt he 8 1 CG EE EE 8 2 Clean Up spilserien eles aer ss 8 2 8 2 viPr veniative ENEE 8 2 8 3 Cleaning the Sample Chamber Lis iesyshcesciar vdiste skadon dho dd 8 2 8 4 Membrane Replacement sai savesusnz s ise 8 3 Ga U
102. ubstances ccesscccsssceeseececeeececseeeecseeeeseeesneeecseeeeenaes 14 3 Physical Damage qind Eden 14 3 Appendix F Temperature Effect on Operation ccccsscssssceesees 15 1 Temperature Effect On Battery Lil s tvec arsi dendi ad du dh den 15 1 Appendix G Accessory Products ees cenon ece ce sene c ooo core ce EE ER KEE 16 1 Appendix H Cleaning Disinfecting and Decontamination 17 1 Appendix I Varranty and Shipping Information cccccccccsee 18 1 enger Tee 18 2 Pacino Instructions nenea Eeer u e E TE E ER 18 3 Appendix J Required Notice seosesseseessoscesecsessoscesessessecseseessoseeseso 19 1 1 Safety 1 1 Explanation of Symbols Warning indicates that misuse of the instrument could result WARNING SE in death or serious injury to a person Caution consult accompanying documents Caution CAUTION indicates that misuse of the instrument could result in mild or serious injury to a person and or damage to equipment gt Sh Biological Risks ual Manufacturer m Authorized Representative in the European Union IVD In Vitro Diagnostic Medical Device REF 2747 Catalog number LOT 03A6549 Lot number YEAR MO Date of manufacture YEAR MO Use by Date Temperature Limitation SH 1 1 2 Introduction 2 1 The YSI 1500 SPORT is a portable battery povvered blood lactate analyzer intended for professio
103. y to return to the Main menu Printing Previous Days Results RUN 0 RECALL 1 SETUP 2 DIAG 3 oO S 5 Lg gt DO E 5 z Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 H PRINT ALL 0 BY DATE 1 ID 2 gt DATE MM DD YY XX XX XX Loi Loi CH CH CH CH O Q J av S af a o Z Z Ke 5 paj paj Lei Lei ke da Lem qt gt gt CH S S ti ti Z Z ti ti za za NOTE Today s date will appear Key in a date as described in Section 3 6 System Setup then press ENTER Example January 17 1998 is entered as 01 17 98 ENTER The display will be active during the printing process showing each result as it is printed It is not possible to halt the printing once it has started Once finished the display will return to the RECALL menu SAMPLES LCD 0 PRINT 1 XMIT 2 Use the MENU key to return to the Main menu Printing Results By ID Number RUN 0 RECALL 1 SETUP 2 DIAG 3 Q sa aa S aa gt e 5 5 z e 5 E 6 17 Select Recall by pressing 1 then ENTER SAMPLES LCD 0 PRINT 1 XMIT 2 Select Print by pressing 1 then ENTER PRINT ALL 0 BY DATE 1 ID 2 Select ID by pressing 2 then ENTER ENTER ID XXX Enter the desired ID as a three digit number from left to right then press ENTER The display will be active during the printing process showing each result as it is printed It is not possible to halt the printi

User`s Manual YSI 1500 Sport

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