EPIMAX™ AUTOMATED PUMP USER MANUAL
Contents
1. Press Selection Key Function Setting Key twice Display will show 01 Using either Fast Setting Keys or Fine Setting Keys adjust the volume until desired volume is reached Press Selection Key to go next setting or press START to initiate the pump volume will flash three times on the display Note e Syringe volume does not need to be changed unless the different volume size syringe is used 7 6 Displaying Total Volume a b EpiMAX Automated Pump To display total volume of buffer pumped into the column press Syringe Volume Key gt at anytime To reset the displayed Total Volume simultaneously press Syringe Volume Key and Mute Key at anytime www epitomics com 6 7 7 System Pressure Selection a b C Press Selection Key twice Display will show OCCL Using Fine Setting Keys select either High H or Low L pressure setting Press Selection Key to go next setting or press START to initiate the pump volume will flash three times on the display 7 8 Automated Control Setup EpiMAX Automated Pump includes a standard RS232 interface For automated control of the pump connect a compatible device Standard IEC6090 1999 through the RS232 interface 8 0 Maintenance Clean Pump Surface Replace Fuse Clean outside surface of pump with soft cloth Use cleaning detergent if necessary Daily Weekly Replacement of Fuse Located rear cover of the pump Loosen screws and remove the fu2. Syringe Holding Clamp clock wise direction to lock the syringe in place Press FAST Key once Press and hold FAST Key again until reagent is coming out of the syringe tip Connect the prepared column to the syringe EpiMAx Automated Pump www epitomics com 5 7 4 Setting Flow Rate Press STOP key to enter STOP Mode Flow rate can be only set during STOP mode Press Selection Key until you enter Flow Rate Setting Mode ml h sign will light up Current flow rate will be displayed on the LED Digital display Using either Fast Setting Keys or Fine Setting Keys adjust the flow rate until desired flow rate is reached Press Selection Key to go next setting or press START to initiate the pump a b C d d 7 5 Setting Display Volume 7 5 1 Setting Reagent Volume in the Syringe a b C Press STOP key to enter STOP Mode Press Selection Key until you enter Volume Setting Mode gt ml sign will light up Current reagent volume will be displayed on the LED Digital display Using either Fast Setting Keys or Fine Setting Keys adjust the volume until desired volume is reached Press Selection Key to go next setting or press START to initiate the pump Note e Volume is locked during START Mode and pressing Selection Key will display the current set volume e To clear preset volume at anytime simultaneously press Mute Key and Function Setting Key Selection Key 7 5 2 Setting Syringe Volume a b C
3. post 7 0 PUMP OPERATION Please read through entire procedure before beginning 7 1 Charging the Battery a Connect the pump to an AC power source The AC power indicator will light and pump will go into charge mode Before the first use allow the battery to charge at least 16 hours 7 2 Power On and System Self Check a b C Press and hold Power Key for 2 seconds to turn on the pump Pump will enter Self Check Mode Check LED display for any error messages If there is no error message displayed the pump is fuctional and ready to be used If there is any error message please refer to the Alarm Reference Troubleshooting Section Section 9 for apporiate action 7 3 Syringe Installation groove Fixed groove syringe stool push rod Figure 3 Fixed groove for Syringe Body and Plunger j Pull the syringe plunger and allow apporiate amount of liquid reagent to enter the syringe Remove air bubbles in the syringe by dislodging the bubbles with gentle tapping and pushing the excess air with syringe plunger Turn the Syringe Holding Clamp couterclock wise direction to expose the Syringe Bed While pushing the Rocking Plate on the Pump Head push the Pump Head all the way to the right Insert the reagent loaded syringe into the Syringe Bed and secure it into the Fixture Groove for Syringe Body and Syringe Plunger Head Figure 3 Place the Pump Head on top of Syringe Plunger Head Turn the
4. syringe bed EpiMAX Automated Pump will automatically detect the syringe volume and adjust the flow rate and volume range to optimal level Enter desired output volume and flow rate EpiMAX Automated Pump will start to deliver desired volume at preset constant flow rate As a preventative feature the following alarm settings are built in to EpiMAX Automated Pump to maintain optimal performance e When remaining buffer in the syringe is less than 1 5ml the Near Empty Alarm Indicator alarm will be activated e After the last 1 5ml buffer is delivered Empty Volume will be activated and the pump will automatically stop e When pump pressure builds up as a result of column clogging tubing closure Clogging over pressure alarm will be activated and the pump will automatically release the pressure and stop e During loss of power e g low or dead battery lost of power source either Low Battery Voltage Alarm or Power Alarm will activate automatically saving previous settings e g flow rate volume level and shuts down EpiMAxX Automated Pump www epitomics com 3 4 0 PACKAGE CONTENTS The following components are included in the box Automated Pump e Power Cord e 5 0 TECHNICAL SPECIFICATIONS Description Syringe Adaptability Specification Standard 20 30 50 ml Syringes Clamp Hold Set Clamper Holder Screws Mini Wrench Pump Document User Manual Quick Guide Quality Certification Notes Volume
5. 2622 support epitomics com Catalog Product Name Size N A EpiMAX Affinity Purification Kit 10 0 RELATED PRODUCTS 6000 1 11 0 CONTACT Epitomics Inc 863 Mitten Road Suite 103 Burlingame CA 94010 1303 U S A Phone 650 583 6688 Fax EpiMAx Automated Pump 650 583 6680 www epitomics com 877 772 2622 Toll Free North America 13 0 DISCLAMINER WARRANTY Disclaimer Epitomics Inc products are warranted to meet the applicable product specifications described Notice of non conforming products should be made to Epitomics Inc within 21 days of receipt of the product Epitomics Inc disclaims any and all responsibility for any injury or damage which may be caused by the fault of the user or the buyer in accordance with the terms and conditions herein WARRANTY COVERAGE Epitomics warranty obligations are limited to the terms set forth below Epitomics Inc Epitomics warrants this hardware product against defects in materials and workmanship for a period of ONE 1 YEAR from the date of original retail purchase If a defect exists at its option Epitomics will 1 repair the product at no charge using new or refurbished replacement parts 2 exchange the product with a product that is new or which has been manufactured from new or serviceable used parts and is at least functionally equivalent to the original product or 3 refund the purchase price of the product A replacement product part a
6. EPITUMICS The Rabbit Monoclonal Company EPIMAX AUTOMATED PUMP USER MANUAL Rev 2 0 CAT ASP D100NA ASP D100EA Published August 1 2007 EPITOMICS EPIMAX AUTOMATED PUMP USER MANUAL 1 0 2 0 3 0 TABLE OF CONTENTS 2 0 Introduction p 2 3 0 Pump Features p 2 4 0 Packing List p 3 5 0 Technical Specification p 4 6 0 Pump Installation p 5 7 0 Pump Operation 7 1 Charging the Battery p 5 7 2 Power On and System Self Check p 6 7 3 Syringe Installation p 8 7 4 Setting Flow Rate p 6 7 5 Setting Displaying Syringe Reagent Volume p 6 7 6 Displaying Total Volume p 6 7 7 Setting System Pressure Level p 6 7 8 Automated Control Setup p 7 8 0 Maintenance p 8 9 0 Alarm Reference and Troubleshooting Guide p 9 10 0 Related Products p 9 12 0 Contact Info p 9 13 0 Disclaimer Warranty p 10 INTRODUCTION EpiMAX Automated Pump is a digital fixed volume pump featuring high precision control and timing for consistent and wide allowance range of flow rates ideal in protein chromatography and related applications EpiMAX Automated Pump is compatible with 3 syringe volume settings 20ml 30ml and 50ml and it is optimized to be used with EpiIMAX Affinity Purification Kit Cat 6000 1 for a complete automated antibody purification system PUMP FEATURES EpiMAX Automated Pump features micro propulsion system for precise pump volume and consistent flow rate EpiMAX Automated Pump is a fixed volume pump and unlike constant pr
7. Limit 0 9999 ml Below 1000ml 0 1 ml increments Above 1000ml 1ml increments Accuracy lt 2 Drive accuracy 1 Clogging Over Pressure Low Pressure L 40N 10N High Pressure H 60N 10N Alarms Power Near Empty Volume NEA EMPTY Empty Volume Improper syringe clamping Wrong clamping of syringe plunger Malfunction or missing operation after initiation Clogging over pressure Output volume equal to preset volume Low battery Power failure AC230V 50 60HZ For Europe amp Asia ASP D100NEA AC110V 50 60 For North America ASP D100NA DC12V The pump can run continuously for over 4 hours at the rate of 5ml h with internal battery after 16 hours of charging Operation Condition Temperature 5 to 40 C RH 20 to 90 Transportation and 40 to 55 C RH lt 95 Storage Dimensions 307 L x135 D x128 H mm Pump Rate Syringe Volume 20ml 30ml 50ml Flow Rate 0 1 399 9ml h 0 1 399 9ml h 0 1 399 9ml h Rapid Rate 399ml h 600 0ml h 1200 0ml h EpiMAx Automated Pump www epitomics com 6 0 PUMP INSTALLATION EpiMAX Automated Pump can be installed horizontally or vertically to a supporting pole by using the clamp located on the back of the pump Figure 2 Figure 2 Clamp Setup for EpiMAX Automated Pump i i Fixed clamping figure of Fixed clamping figure clamp hold i ping 1g of vertical post horizontal
8. essure pump e g creeping pump the flow rate and set volume is not affected by column pressure When the maximum pressure set level reached EpiMAX Automated Pump will automatically stop and notify the user to release the column pressure preventing alteration of column flow rate and set volume EpiMAx Automated Pump www epitomics com 2 PE es ae Rs nem ra rg mays est gh ment gig ym emi pe lg key Si new ln re any pT yn any oS mdi ae Alarm indicator Clogging over pressure Display of pressure LED digital display Alarm indication for empty Selection key 1 Indication for Function setting key Alarm indication near empty Improper syringe clampling Mute key Syringe volume Pump running indicator 5 inquiry key ITWMIC indication for syringe size i 20 s 0 Indication for bat tery weve U O Indication for DC Indication for AC Affinity Purification System h 0 Fast advance key Pump head c Rocking plate Fixture groove for syringe Fast setting keys plunger head on pump head Fine setting keys Syringe plunger head Power switch Syringe holding clamp Syringe plunger Syringe bed Fixture groove for syringe body Fixture groove for syringe body Figure 1 EpiMAX Automated Pump Setting up in EpiMAX Automated Pump is fast and simple with Auto Syringe Detection When a syringe with specified volume 20 30 or 50 ml is attached to the
9. or less of operation time Recharge battery No Power from Battery Light On No Battery Power Recharge Battery Alarm Beeps Twice after initial start up No operation of the pump initiated after 2 minutes No Action Needed EpiMAx Automated Pump www epitomics com Problem Solution Inaccurate rate The edge of syringe body is not properly connected to the groove fixture on the pump Follow correct procedure Unrecognized syringe Use standard disposable syringeswith volume setting 20 30 or 50 mls Alarm for the Low Battery upon startup The battery is charged after previous Use or placed for a long time after charging Recharge battery immediately Improper use of build in battery the battery is damaged Replace the battery Buffer back flow not flow upon Start Before inserted needle tip into vein mechanism gap is not cancelled by pressing FAST key Confirm no air is tapped in the tubing Press FAST key and force buffer intoTubing The edge of syringe body is not properly connected to the groove fixture on the pump Follow correct procedure Tubing clogging Replace tubing Advancing Head Unsmooth movement of Clamp block is stuck Clean clamp block with alcohol e g 10 Isophrophol For additional information and technical inquiries please visit Epitomics website or contact technical services at 877 772
10. se holder Replace with 0 5A 5x20 Fuse As Required Replace Battery Contact Epitomics As Required EpiMAx Automated Pump www epitomics com J Alarm NEA EMPTY Near Empty Light On Cause Reagent volume in the Syringe is less than 1 5 ml 9 0 ALARM REFERENCE TROUBLESHOOTING GUIDE Solution Press STOP Key and refill the syringe with reagent if necessary EMPTY Light On Reagent volume in the Syringe is at zero Press STOP Key and refill the syringe with reagent if necessary Clogging Over Pressure Light On Column Clogged Preset pressure level reached Note with the increased output volume the pressure of the system will increase gradually When system pressure of the reaches preset pressure value Alarm for Clogging Over Pressure will be triggered Press STOP key and release the pressure change column or unclog column Press MUTE Key to reset the pump Improper Syringe Clamping Syringe Holding Clamp is not properly set Syringe is not securely set into the Syringe Bed and or Fixture Groove Body or Head Re secure Syringe onto the Syringe Bed Fixture Groove Recheck Syringe Holding Clamp AC Power Failure On Lost of AC Power to the Pump Check if the power cord is securely connected to the pump and to the power source Check if power source is active Low Battery Light On Low Battery Level battery level is good for only 30 minutes
11. sonal use so long as you do not remove any copyright or other notice as may be contained in information as downloaded Trademark Notices Epitomics Epitomics symbol logo are the worldwide registered trademarks and service marks of Epitomics Inc Other featured words or symbols used to identify the source of goods and services may be the trademarks of their respective owners EpiMAx Automated Pump www epitomics com 10
12. ssumes the remaining warranty of the original product or ninety 90 days from the date of replacement or repair whichever provides longer coverage Any shipping and handling charge incurred for any replacement repair and or exchange will be the responsibility of the customer When a product or part is exchanged any replacement item becomes the customer s property and the replaced item becomes Epitomics property When a refund is given the product becomes Epitomics property All components of the pump except for the battery is covered by this warranty Technical documents listed in IEC60601 1 term 6 8 3C as upon customer s request A 0197 Rheinland Product Safety Copyright Notices The works of authorship contained in Epitomics Website including but not limited to all design text images and scientific data are owned except as otherwise expressly stated by Epitomics Inc and its subsidiaries Except as otherwise expressly stated herein they may not be copied transmitted displayed performed distributed for compensation or otherwise licensed altered framed stored for subsequent use or otherwise used in whole or in part in any manner without Epitomics Inc s prior written consent except to the extent permitted by the Copyright Act of 1976 17 U S C Section 107 as amended and then only with notices of Epitomics Inc s proprietary rights provided that you may download information and print out hard copies for your per
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