Flow Controller Users Manual - Flexcell International Corp.
Contents
1. OsciFlow Setup and Assembly cont Figure 1 OsciFlow front with tubing connector in place Connecting the OsciFlow to your computer Follow the software installation instructions on Page 7 to install the StreamSoft software Insert the USB cable into an available USB slot on the side of your computer Connect the other end of the USB cable to the back of the OsciFlow Plug the OsciFlow in and turn the power on HP p YP Once the system is fully connected clean it by pumping deionized water through all components Note Any of the tubing lengths above can be shorted or extended according to your setup needs STREAMSOFT V4 2 SOFTWARE 6 OSCIFLOW USER MANUAL StreamSoft V4 2 Software Installation Instructions 1 2 3 4 Insert the StreamSoft V4 2 DVD into the DVD ROM drive on the computer Double click My Computer Windows XP or Computer Windows Vista Windows 7 Double click the DVD ROM drive Double click the Setup installer The installer will now open and run 5 6 7 8 9 On the Product Notification screen click Next On the Destination Directory screen click Next On the License Agreement screen click I accept the License Agreement and then click Next On the next License Agreement screen click I accept the above 2 License Agreement s and then click Next On the Start Installation screen click Next Installation of the required National Instruments and StreamSo2. Cancel Cancel any new selections and use the previously configured setup for the experiment Online help not currently available Pull down Menus This section summarizes the function of each item in the three pull down menus Operate Manual Mode Help View Data About LabVIEW Users Configure Regime Configure Apparatus Configure System Reinitialize Hardware File Print Allows user to print a copy of the current panel This system is configured such that printing sends a copy of the panel being viewed to a printer or to an html file If there is no printer connected to the computer an error message from the Windows default printer queue will appear when the user tries to print Exit Allows user to close the program If the pump is operating at the time of exit it will continue running The keyboard short cut is Ctrl Q STREAMSOFT V4 2 SOFTWARE 11 OSCIFLOW USER MANUAL Operate Manual Mode Manually control the pump without setting up an experimental regimen View Data View shear stress data from a previous experiment Users Add and remove user names Configure Regime Create an experimental protocol Configure Apparatus Configure parameters of the flow device so that the software can assign the flow rates corresponding to the desired shear stress These parameters must be set correctly to ensure that the proper shear stress values are shown See the manual of y
3. pump speed either by entering a number in the box or using the mouse to drag the dial to the desired level 2 Adjust the seconds between readings to a number between 0 and 5 This is the time between each update of the pump data on the graph Click on Press to Start 4 Click on Press to Stop when ready to stop 5 Click on Return when done STREAMSOFT V4 2 SOFTWARE 13 OSCIFLOW USER MANUAL View Data Function This panel allows the user to view previously collected experimental data in a table format gt View Data File h C Program Files Streamer rev 2 2 data testdata Test 1 Streamer L S 17 6 000000 2 340000 aaa Viscosity dyne s cm 2 0 010000 Data Updates s 11 0 Delay between File updates s 5 0 1 22 2003 4 16 11 PM Expected Shear From Pump 0 09 0 00 10 00 9 99 10 00 9 99 Export File Print Open Return Help Buttons and Fields The complete file path to the data file being viewed Contents of the experimental data log file Export File Export data to a spreadsheet compatible format Print a copy of this panel to the Windows default printer or write a copy to an HTML file Open a data file Close this panel and return to the Main panel Online help not currently available STREAMSOFT V4 2 SOFTWARE 14 OSCIFLOW USER MANUAL Configure Users Fu
4. Box Weight 14lbs 6 4kg e Power Requirements 120V 0 15A e Tubing sizes accepted MasterFlex L S 16 L S 25 L S 17 e Interface Connection to computer USB OsciFlow Components e OsciFlow Device e USB cable e OsciFlow Tubing includes quick disconnect fittings e Additional tubing lengths necessary for connecting to flow system e StreamSoft software V4 2 e Dell Inspiron notebook computer optional OsciFlow Setup and Assembly The following instructions are for integrating the OsciFlow into the Streamer and or FlexFlow systems When using the OsciFlow with either system the box should not be placed in the incubator with the flow device and its other components The OsciFlow should be placed on top or beside the incubator as near as possible to the port through which the tubing enters the incubator This will minimize the amount of fluid that is exposed to atmospheric temperatures STREAMSOFT V4 2 SOFTWARE 4 OSCIFLOW USER MANUAL OsciFlow Setup and Assembly cont The OsciFlow is very easily integrated into the Streamer and FlexFlow systems A special tubing connector is provided with the OsciFlow that will connect directly in line with the Streamer or FlexFlow system with the addition of two more tubing lengths provided with the OsciFlow 1 Connect the OsciFlow tubing connector to the front of the OsciFlow as shown below Push the tubing all the way into the
5. FWD mode and the second in REV mode Each step should be 2 minutes at a shear stress level Y2 that of which your device is capable This will give sufficient time for fluid to fill all of the tubing As you notice air bubbles in the silicone tubing at different locations shake the tubing to release the air bubbles STREAMSOFT V4 2 SOFTWARE 25 OSCIFLOW USER MANUAL OsciFlow Troubleshooting When the OsciFlow valves are not responding When the OsciFlow valves do not respond the following troubleshooting steps should be taken e Check that the OsciFlow is powered on The red light on the front of the box should be illuminated e Check all connections to be sure that they are intact one end of the USB cable should be fully inserted into the USB slot on the side of the computer and the end should be connected to the back of the OsciFlow e Check that tubing has been inserted into the OsciFlow pinch valves If there is no tubing in the valves they will not return to their off position and the device will appear to be malfunctioning e Stop and restart the software The OsciFlow valves should click as the software is initializing If the valves do not click open the Operate menu in the software and select the Configure System option Make sure that the Valves in System option is selected If not select this option and click Return e Ifthe valves do not click at this point proceed with the following s
6. the Testing Apparatus being used for the experiment or enter a name for a new apparatus in the Name box 2 Enter the correct flow factor for your device This specifies the number of parallel flow chambers in your device 3 Select the correct Hose Size for the type of Masterflex tubing being used in the experiment 4 Enter the proper b and A values for your device Enter the Viscosity of the perfusate fluid used in the experiment The default value is 0 01 dynes s cm 6 Click Save Apparatus button then click Return to exit this screen Configure System Variables Function This panel is used to select three system parameters Communications port data saving and the presence of valves in the flow system Streamer Pulsed Flow Version 2 2 0 a File Operate Help General Information System 1 ystem2 Systen System 4 Status uu a User Demo Rosine Test SYSTEM 1 Save Data x Valves In System a lt S E E D 74 0 00 16 16 37 21 16 16 37 50 16 16 38 00 16 16 38 50 l 46 16 39 21 01 22 2003 01 22 2003 01 22 2003 01 22 2003 01 22 2003 Time y ES LA Elapsed Time mi Saja im Loo Pulse Ste Direction Shear dynes cm 2 al fey 1 14 1 REV STREAMSOFT V4 2 SOFTWARE e 21 OSCIFLOW USER MANUAL Buttons and Fields Pump Serial Com 1 is the default port This should be changed only if there is a conflict Port with this port on your computer Selec
7. 100 Delete Step Delete Regime TT Check Shear STREAMSOFT V4 2 SOFTWARE 16 OSCIFLOW USER MANUAL Buttons and Fields Existing Users List of all users select a user from the list using the mouse Regimes for List of regimens created by the current user Selecting from this list will Selected Users load that regimen and allow the user to view and or modify that regime Name of the current regimen if creating a new regimen enter a name in Regime Name this field aime Between Time elapsed between computer updates of the pump parameters default is 1 second Pump Updates Time interval between each computer sampling of the experimental flow data Default value is 10 seconds For an extremely long test increase this interval to reduce the size of the data file Note this function only applies when the data saving option is selected in the Configure System Variables window See Pages 21 and 22 Time Between Data Log to File This is an estimate of how large the data file would be given the total test Estimated file length and the time between data Note this function only applies when size the data saving option is selected in the Configure System Variables window See Pages 21 to 22 Current step number selected or being modified Name of the currently selected step Specifies the direction or type of flow for this step forward reverse Flow Type ee pulsed square wave oscillation When using pu
8. 4 USA only Fax 919 732 5196 Email info flexcellint com Web www flexcellint com Europe Dunn Labortechnik GmbH Thelenberg 6 56567 Asbach Germany Phone 49 2683 43094 Fax 49 2683 42776 Email info dunnlab de Web www dunnlab de Japan LMS CO LTD 3 6 7 Hongo Bunkyo ku Tokyo 113 0033 Japan Phone 81 3 5842 4171 Fax 81 3 5842 4180 Email intldpt Ims co jp Taiwan Nature Opera Biotechnology Inc 9F 2 No 70 Sec 4 Cheng Kung Rd Nei Hu Dist Taipei Taiwan Phone 886 2 27905097 Fax 886 2 2793 1322 Email nobio seed net tw Brazil Sellex Inc 5225 Wisconsin Ave NW Suite 306 Washington DC 20015 Phone 5506 4646 Fax 5505 7433 Web www sellex com China Hong Kong Malaysia Bio Excellence International Tech Co Ltd Email slby800 yahoo com Web www bio goods com STREAMSOFT V4 2 SOFTWARE 31
9. Devices and Interface Digital 1 0 1 Select Port Port Name porto X Counter 1 0 2 Select Direction Port Line Direction pl 5 port0 Direction 11111111 7 0 3 Select State Port tine State port0 State 00000000 7 0 gt Start ae Y portO lineO 7 Input 1 Output 0 7 Gime Gamma portO lineO 7 High 1 WA EA 7 2 2 Show Help 0 o 0 In 2 Select Direction mouse click Output 0 numbers 7 6 5 and 4 then click Start At this point two of the valves should click STREAMSOFT V4 2 SOFTWARE e 27 OSCIFLOW USER MANUAL OsciFlow Troubleshooting x NI USB 6501 Devi onfi ti amp My System Data Neighborhood a amp Devices and Interface 4 NI DAQmx Devic YA NIUSB 6501 PX PXI System Unidi 44 Scales Sd Software Remote Systems H Test Panels NI USB 6501 Dev1 Digital 1 0 Counter 1 0 1 Select Port Port Name portO 2 Select Direction Port Line Direction port0 Direction 00001111 vA 0 3 Select State PortAine State portofined 7 gt port0O line0 7 Input 1 530 00 o Output 0 E DE 7 0 _ allinput _Al output portO lineO 7 mo ana 7 0 AHigh alltow portO State 00001111 7 0 Show Help 8 In 2 Select Direction mouse click All Input to release the valves to their de energized state 9 Once th
10. FLOW USER MANUAL Resume Resume a paused experiment Graph This graph shows the expected and actual shear stresses during the P experiment Elapsed Time h m s Elapsed time in the current experiment Current loop in the current step or series of steps Pulse Total number of pulsations square wave or oscillations e FWD REV produced by the valves in this regime Current active step in regime Current flow direction FWD REV Pre Test Configuration Function This panel allows the user to configure the parameters of an experiment It appears when the user presses the Configure button on the System panel The information selected here is transferred to the User Regime and Apparatus fields on the System panel gt Pre Test Configuration O A Apparatus FlexFlow Streamer Print Cancel Help STREAMSOFT V4 2 SOFTWARE 10 OSCIFLOW USER MANUAL Buttons and Fields List of all users Select users with the mouse List of regimes created by the previously selected user Select from list by Regimes using the mouse to highlight the desired regime List of configured flow devices Select the device that will be used for the experiment Apparatus Important Be sure that all parameters have been properly set for your device in the Configure Testing Apparatus window See Pages 19 to 21 Print the current panel to a printer or HTML file Use the current selections to run the experiment
11. Osci Flow Flow Controller Users Manual FLEXCELL INTERNATIONAL CORPORATION 2730 TUCKER STREET SUITE 200 BURLINGTON NC 27215 USA 800 728 3714 USA ONLY OR 919 732 1591 WWW FLEXCELLINT COM DECEMBER 2014 Copyright O 2004 Flexcell International Corporation OSCIFLOW USER MANUAL Table of Contents TABEE OF CONTENTS ct Oia ce naci Ro tadas oReaoidciaiioiossses 2 GETTING STARTED 00 incuseessaChaedesosssvvacosssdsednsotebssesesouieussesesesasusesenscesesceecnsecntscbesssietoceees 3 INTRODUCTION ait 4 OSCIFLOW SYSTEM SPECIFICATIONS cssccssecssesssscsssecsseccsuccsuccssecssscssucssucssscssscesuccsneesuscssecssecssucssuesssecssucesucsssecssesstessssssecesseees 4 OSCIFLOW COMPONENTS ecsssesssecssuccsnccssecssecssucssuesssecesucssucssnccsnscsnecssucssucssscssecssucssucssuecssecsuecssusssucsssssssecssucsasesssecsuesssesssuessseees 4 OSCIFLOW SETUP AND ASSEMBLY cccssccssccsseccsscssuccssecssscssuccsuecssscssscssucssucssscssscesucssusessscssecesucssucssucsssucssecssucesaesssecstesstesstecssseees 4 System Taby in per a E E E N E EE dative tava EE Ea SEPE T Ea EE EEEE EE ESEESE EE KEEFE EEEE SE 9 Pre Test CONSI A E NO EETA N E 10 Pulledo wn Menis erone iin e EE TORE EAE EE E E A AWA AAA 11 OPERATE MENU sw ea erreen eaae T Naa r ORe WANAE Eao Ae E PEES Ka AE NERE EAEN Aaaa 13 Manual A LO S AEREE REE aer id 13 RATANA DA AAA WA AUA E E E 14 Conf sure Users Ai 15 Configure Regimes Setup Parameters A A 16 Configure Tes
12. abs will automatically become highlighted according to the AA number of pumps connected to the computer 1 4 General Information Current date and time STREAMSOFT V4 2 SOFTWARE 8 OSCIFLOW USER MANUAL System Tab Function This panel is used to run the experiments Each System tab is identical File Operate Help Status all a al a General Information System 1 User Demo P Regime Test 1 SYSTE M 1 Resume Apparatus Streamer Pause Expected Pump Feedback Data File testdata Shear dynes cm 2 0 00 16 16 37 21 16 16 37 50 16 16 38 00 16 16 38 50 01 22 2003 01 22 2003 01 22 2003 01 22 2003 01 22 2003 Time Time ajali Elapsed Time 4158 0 E h m s Loop Pulse Step Direction Shear dynes cm 2 l sjey 28 a JJ pu E REY 16 16 39 21 Buttons and Fields Current user in currently configured regime Regime currently configured Device being used with the software Streamer or FlexFlow Name of file to which data is being saved if appropriate Configure load the experiment The Pre Test Configuration onfigure Cong window will appear Start Start the experiment Sto Terminate the experiment This button is only active when an p experiment is running Paue Suspend the experiment The pump will stop but the test regimen is kept in memory STREAMSOFT V4 2 SOFTWARE 9 OSCI
13. at the pump or valve timing could be interrupted This effect may be noticeable when using the OsciFlow at a higher frequency than 2 seconds on 2 seconds off or when oscillating the pump speed to create pulsatile flow e When the Select Pump to Use window appears when opening the StreamSoft V4 2 software select the pump named MasterFlex Peristaltic Pump to ensure correct function of the equipment STREAMSOFT V4 2 SOFTWARE 23 OSCIFLOW USER MANUAL Doing an experiment Creating a Regime 1 2 3 A 5 From the main panel select the Configure Regimes item in the Operate menu Click on an existing user name To create a new regimen click on New Regime Enter a name in the Regime Name field Click Insert Step give this step a name in the Step Name field Click on Save Regime The regime name should appear in the Regimes for Selected Users field at the top Specify the Flow Type FWD REV PULSED OSCILLATION ON HI amp OFF LO times only when using the pulsed or oscillation functions see Page 17 for more details Shear and Duration for this step Click on Save Regime to save all information entered up to this point Note If you are using the OSCILLATION function create a first step in the regime in FWD mode This step is required to push out the air that remains in the top of the Streamer after the top is opened for slide insertion The first step will push this air through to the outlet in the mediu
14. atus is selected this field and the parameters will pani be updated A factor that accounts for any parallel paths in the flow stream This Pion Bantur number is 6 for the Streamer and 1 for the FlekFlow Hose size determines how fast the pump must move to achieve the desired flow rate and shear stress level The sizes listed are standard for Masterflex tubing Select the hose size that you are using with your system Hose Size Width of the flow area cm in a single chamber of the Streamer or FlexFlow device This number is found in the back of the manual for your device listed as Flow Area Width cm Height of the flow area cm in a single chamber of the Streamer or FlezFlow device This number is found in the back of the manual for your device listed as Flow Area Height cm Viscosity of the perfusate media used in the experiment The standard value is 0 01 Viscosity Print the current panel to a printer or an HTML file Save Apparatus Save changes to the apparatus listed in Name Delete Apparatos Deletes apparatus listed in Name Online help not yet available Exit this panel and return to the Main panel Any changes that have not Return been saved will be discarded STREAMSOFT V4 2 SOFTWARE 20 OSCIFLOW USER MANUAL How to enter the proper values for your device Please check the Appendix of the manual for your device for the proper b and h values 1 Select
15. ce center or distributor with all shipping costs prepaid if the unit is out of warranty coverage If the original shipping box is not available Flexcell will send the required protective shipping container Flexcell will recommend the insurance value for parts or equipment to be shipped Return carrier shipping costs will be paid by Flexcell from the service center The purchaser customer is solely responsible for payment of custom fees taxes holding fees or value added taxes THIS LIMITED WARRANTY only covers failures due to defects in materials or workmanship which occur during normal use It does not cover damage which occurs in shipment or failures of original equipment due to products identified as add ons not manufactured by Flexcell International Corporation or its distributors nor does this limited warranty cover damages or failures which result from accident or disaster such as fire explosion flood wind lightning or earthquake or misuse abuse neglect mishandling misapplication alteration faulty installation modification or service by anyone other than our factory or distributor This warranty is extended only to the original purchaser customer unless a transfer of ownership is approved by Flexcell in writing LIMITED LIABILITY Flexcell or its distributor s only liability shall be to remedy any defect to comply with its warranty and return the repaired equipment to function as designed Under no circumstances shall Flexcell or its di
16. e valves are in their de energized state click Stop If the OsciFlow does not respond in the above procedures the unit should be returned to Flexcell for repair STREAMSOFT V4 2 SOFTWARE 28 OSCIFLOW USER MANUAL Warranty Information 1 FLEXCELL INTERNATIONAL CORPORATION warrants to the original purchaser customer all hardware components of the OsciFlow System for a period of one year from the date of delivery to the purchaser customer to be free from manufacturing defects in workmanship or materials with the following exceptions terms and conditions a ITEMS EXCLUDED FROM THE WARRANTY ARE software disks manuals and external peripherals such as printers mouse or track ball units imaging devices vacuum pumps air tanks electric voltage converters compressors surge suppressers and all other accessory equipment DURING THE WARRANTY PERIOD the purchaser customer must notify Flexcell of any warranty claim in writing by telephone fax transmission or email identifying each defective part or specifically describe the exact problem no later than the last day the warranty is in effect FLEXCELL AGREES to correct any defect in workmanship or material and supply new or rebuilt parts in exchange for defective parts upon completion and submission by purchaser customer of a printed Parts Return Authorization form furnished by Flexcell Parts must be properly packed in original container and shipped to our factory servi
17. ft software will now begin 10 11 12 13 Once the installation is complete click Finish and restart the computer Turn on the OsciFlow and connect the USB cable to the back of it Insert the opposite end of the USB cable into an open USB slot on the computer The computer should make a sound recognizing that the OsciFlow has been found and the correct drivers will install Installation of StreamSoft V4 2 is now complete Note When the Select Pump to Use window appears when opening the StreamSoft V4 2 software select the pump named MasterFlex Peristaltic Pump to ensure correct function of the equipment Setting up parameters in StreamSoft V4 2 Specific parameters will need to be set up in StreamSoft V4 2 to customize it for your particular device and system Setting up these parameters is extremely important to ensure accurate flow results for your system For instructions on setting up these parameters see Configure Testing Apparatus and Configure System Variables Pages 19 to 22 Complete this setup before proceeding with any experiments STREAMSOFT V4 2 SOFTWARE 7 OSCIFLOW USER MANUAL Main Panel General Information Tab Function The default main panel allows the user to verify that the system is running and to stop the tests at any time i StreamSoft Version 4 2 Status A Buttons and Fields Status Number is bright green when an experiment is running These t
18. hicle for ions proteins carbohydrates and other molecules capable of movement within the matrix As the fluid moves past cell membranes a shear stress t is generated If one assumes that laminar flow occurs through a parallel plate flow chamber fluid induced shear stress values can be determined with the following formula t 6uQ bh where 1 is the shear stress in dyne cm p is the viscosity of the fluid in dyne s em Q is the flow rate in ml s b is the width of the flow channel in cm and h is the height of the flow channel in cm Shear stress in the vascular system may vary from less than 1 to more than 35 dyne cm Fluid shear stress in canaliculi of bone may vary from 1 to 20 dyne cm while in cartilage it may be in the range of 1 to 5 dyne cm The OsciFlow is a software controlled valve operated device designed to provide oscillatory or start stop flow patterns to cells cultured in the Streamer FlexFlow or other perfusion devices Both the Streamer and FlexFlow are parallel plate flow systems designed to apply fluid induced shear stress to cells grown in a monolayer The OsciFlow regulates flow direction by choreographing the closing and opening a collection of pinch valves This carefully timed action serves to constrict or release tubing thus directing the fluid flow through the desired channels The OsciFlow device is able to regulate flow both in an oscillatory flow reversal manner and in a pulsatile square
19. lete the currently selected regime Save a new or modified regime Exit this panel and return to the Main Panel Check Shear Check the shear stresses entered in your regime to see if they are achievable with the apparatus pump and tubing size that you are using Print the current panel to a printer or an HTML file Online help not currently available Instructions on how to set all the parameters for an experiment are included in the Doing an Experiment section of this manual STREAMSOFT V4 2 SOFTWARE 18 OSCIFLOW USER MANUAL Configure Testing Apparatus Function This panel allows the user to create modify or delete a testing apparatus Streamer or FlexFlow flow chamber As each Streamer and FlexFlow device is manufactured to strict dimensional specifications the values for the height and width of the chambers must be entered into the software for each individual device These values are measured for your specific device and must be correct for accurate shear stress measurement The values can be found in the appendix of the manual for your device B Configure Testing Apparatus Streamer eoo ILs o 2 340000 20 051300 20 010000 o print Seve Apparatus PI Dotto Apparatus BY Hew M Ren STREAMSOFT V4 2 SOFTWARE 19 OSCIFLOW USER MANUAL Buttons and Fields List of all flow devices available Apparatus When a testing appar
20. lsed square wave or oscillatory flow specifies how long ON HI s the valves remain in a position to allow the fluid flow to continue unhindered or flow in the forward direction respectively For normal forward or reverse unidirectional flow this value remains at 1 00 When using pulsed square wave or oscillatory flow specifies how long OFFILO s the valves remain in a position to stop the fluid flow to the device or cause it to flow in the reverse direction respectively For normal forward or reverse unidirectional flow this value remains at 1 00 M M The value of shear stress to be applied to the cells in this step Peon Time to spend in this step hours minutes seconds milliseconds h m s ss P P STREAMSOFT V4 2 SOFTWARE 17 OSCIFLOW USER MANUAL To create a loop indicate which step to go back to The GoTo step must Geto always be a step number before the current step Indicates how many times to loop between the GoTo step and the current Loop step This table is a listing of the current steps in the regimen Selecting a row Summary Table from this table will allow the parameters of the step to be viewed and modified Insert Step Insert a step into the regimen before or after the current step Delete Step Delete the currently selected step Clear all parameters and start a new regime Type in a new name under New Regine Regime Name and select Insert Step Delete Regime De
21. m bottle If you wish to add additional steps 7 Click Insert Step You will be queried as to whether this step should be inserted before or after the current step Click on before or after according to your preference Enter the preferred parameters as in 6 If this step was inserted after the step entered in 6 you can also use the GoTo and Loop options to loop through steps 1 and 2 Under GoTo in step 2 enter 1 Under Loop enter the number of times that you would like to loop through steps 1 and 2 Add additional steps as desired Once the regime is complete click on Save Regime Optional Check the shear stress es in your regime to be sure that they are achievable with your apparatus tubing size and pump Click on Check Shear at the bottom of the Configure Regimes window while your regime is selected see Pages 13 to 16 The Pre Test Configuration window will appear see Page 10 Select a user regime and apparatus Click on Update The software will tell you if your shear stresses are achievable with this apparatus and the tubing size and pump assigned to it Modify shear stresses if necessary 10 The regime is now ready to run STREAMSOFT V4 2 SOFTWARE 24 OSCIFLOW USER MANUAL Filling the System to Eliminate Air Bubbles Before using the OsciFlow system with cells all of the tubing must be filled with media and all air bubbles removed To fill the system create a regime with two steps the first in
22. nction This panel allows the user to create or delete users gt Streamer Pulsed Flow Version 2 2 0 File Operate Help General Information System 1 User Regime Apata Existing Users Data File te Shear dynes cm 2 1 Add User Delete User Help Return 16 16 39 21 01 22 2003 fme alaja Elapsed Time HEA m Time Ajaj m Loo Pulse Ste Direction Jo Shear dynesfcm 2 Alu lr 1 14 1 REV Buttons and Fields Existing Users Lists all current users of the system User Name Field used to enter new users Add User Add new users to the system Delete User Delete users from the system Help Online help not currently available STREAMSOFT V4 2 SOFTWARE 15 OSCIFLOW USER MANUAL Return Exit this panel and return to the Main panel Instructions To add a user 1 Type the name into the User Name field 2 Press the Add User button To delete a user 1 Using the mouse select the user from the list of Existing Users 2 Press the Delete User button If the user has any stored regimes and data sets the operator will be prompted to confirm the deletion Configure Regimes Setup Parameters Function This panel allows the user to configure create a regime Configure Regimes Demo Mame Oscillate 1 4 00 01 00 000 500 00 00 10 000 00 00 10 000 5 00 00 00 30 000 1 2 E E A 5 __ OSCILLATION 0 50 0 50 900 00 00 25 000 REY 1 00 PULSED 100
23. our device for the appropriate values Configure System Configure the system parameters such as data saving the Com port used and the presence or absence of valves in the system OsciFlow Reinitialize Hardware This will reinitialize the software to connect the pump and OsciFlow if present in the event that a cable is disconnected or the pump is turned off Help Help Online help not currently available About LabVIEW Software version information STREAMSOFT V4 2 SOFTWARE 12 OSCIFLOW USER MANUAL Operate Menu Manual Mode Function This panel allows the user to manually control the pump The actual flow rate and speed of the pump RPM are shown on the graph when the pump is working Manual mode may be used to troubleshoot the pump operation The shear stress value is not shown on this panel since it will depend on the tubing size and flow chamber used gt Manual Mode Flow Set Point RPM ColeParmer Drive RPM 49 90 200 0 200 0 4 ya 400 0 400 0 x Y 600 0 600 0 Seconds between readings 2 02 5 3 0 1 5_ Joes 3 5 za 1 1 1 1 1 1 1 1 1 0 4 0 16 30 10 16 30 12 16 30 14 16 30 16 16 30 18 16 30 20 16 30 23 0 57 54 5 01 22 03 01 22 03 01 22 03 01 22 03 01 22 03 01 22 03 01 22 03 01 22 03 7 x it i ue Time E 28 21 time ajui Ed Pl amp pre Time Until Next reading Saud ala sa os s Instructions 1 Enter the flow set point
24. pinch valves so that each piece is fully inserted and pinched between the valve body and pinching mechanism The connector must be inserted as shown below such that the each of the four pieces of tubing is connected to their respective valve 2 Find a location for your OsciFlow that is as near as possible to the port hole on the back or top of your incubator Place the OsciFlow such that the tubing connector quick disconnects are in close proximity to the port hole 3 Disconnect the male quick disconnect that is currently connected to the inlet of your Streamer or FlexFlow device and connect it to the bottom left female quick disconnect refer to Figure 1 of the OsciFlow tubing connector 4 Disconnect the male quick disconnect that is currently connected to the outlet of your Streamer or FlexFlow device and connect it to the top left female quick disconnect refer to Figure 1 of the OsciFlow tubing connector 5 Use one of the tubing lengths provided with the OsciFlow to connect the top right female quick disconnect refer to Figure 1 on the OsciFlow tubing connector to the inlet of your Streamer or FlexFlow device 6 Use the other tubing length provided with the OsciFlow to connect the bottom right female quick disconnect refer to Figure 1 on the OsciFlow tubing connector to the outlet of your Streamer or FlexFlow device STREAMSOFT V4 2 SOFTWARE 5 OSCIFLOW USER MANUAL
25. stributors be liable for any special incidental or consequential damages based upon breach of warranty or contract or negligence Such damages include but are not limited to loss of profits revenue loss of data down time customer s material or time DISCLAIMER OF WARRANTIES The Limited Warranty expressed in the foregoing language is the only warranty applicable to this product Any other warranty expressed or implied warranty or of merchantability or fitness for a particular purpose are hereby disclaimed No oral or written information or advice provided by Flexcell through its agents or employees in the use and functioning of the equipment shall in any way create a warranty or in anyway increase the scope of this limited warranty DISCLAIMER LANGUAGE This warranty document accompanying instruction manual and supplemental applicable laws appear in the English language In the event of any inconsistency in the meaning of the words and terminology and any foreign language translation the English language shall prevail 2 GOVERNING LAW The performance of the duties and liabilities of the parties under the terms and conditions of this Limited Warranty shall be governed in all respects by the laws of the Commonwealth of Pennsylvania the United States of America APPLICATION OF STATE LAWS Some states do not allow the exclusion or limitation of consequential damages nor do some states allow limitations on how long an implied warranty lasts so
26. t this option if you want to save regime data files Select this option if you are using the OsciFlow Flow Controller System Reinitialize Hardware Function This panel will appear when the computer program is first started It will also appear when the Reinitialize Hardware item is selected in the Operate menu When the system is properly initiated the pump will display PO1 and the OsciFlow valves will click If power to the pump is cycled during experimentation or communication is lost the user should reinitialize the hardware before turning off the program and starting it again gt Streamer Pulsed Flow Version 2 2 0 File Operate Help General Information System 1 yster syste ystem 4 Status a a a User Demo Regime Test SYSTEM 1 Apparatus Steamer ti o ao 1 System is initializing hardware Please Wait 0 00 a 1 1 1 16 16 37 21 16 16 37 50 16 16 38 00 16 16 38 50 16 16 39 21 01 22 2003 01 22 2003 01 22 2003 01 22 2003 01 22 2003 Time tm alijua Elapsed Time HAD Er alari im Loo Pulse Ste Direction ji 28 1 14 1 REV STREAMSOFT V4 2 SOFTWARE e 22 OSCIFLOW USER MANUAL StreamSoft V4 2 Notes e When running a regime in StreamSoft V4 2 do not run other applications on the same computer The communication timing to the pump and OsciFlow requires full CPU availability If another program or operation is running that requires CPU power it is possible th
27. teps 1 Close the StreamSoft software so that nothing is running but the Windows environment 2 Disconnect the USB cable from the computer 3 Turn off the OsciFlow 4 Turn on the OsciFlow and reconnect the USB cable to the computer 5 Start the StreamSoft software The OsciFlow valves should click as the computer is initializing the hardware e Ifthe valves do not click proceed with the following steps 1 With the OsciFlow powered on and with all connections intact open up the Measurement amp Automation explorer from the Desktop of your computer If there is not a shortcut on the Desktop check in the folder named National Instruments in All Programs under the Start menu STREAMSOFT V4 2 SOFTWARE 26 OSCIFLOW USER MANUAL OsciFlow Troubleshooting 7 Under Devices and Interfaces click on NI DAXmx Devices should be listed NI USB 6501 Devl Right click on this device and choose Test Panels A warning window will might appear Simply click Yes to continue If a second warning window appears before the Test Panels appear this means that the computer is not recognizing the OsciFlow In this case contact Flexcell Once the Test Panels appear in the Digital I O tab the following information will be shown E NI USB 6501 Devi a EY My System E Data Neighborhood NIUSB 6501 PX PXI System Unidi 44 Scales El Software amp Remote Systems 4 g
28. the above limitations may not apply to you This warranty gives you specific legal rights and you may also have other rights which vary from state to state 3 INTERNATIONAL CUSTOMERS The full text of the foregoing limited warranty and all disclaimers is applicable to international customers purchasers except when the purchase was made from an international distributor or reseller the warranty will be covered through your distributor or reseller If technical advisory support service is not available through your distributor or reseller for service contact warranty headquarters by phone or fax Within the United States only Toll Free 1 800 728 3714 Fax 1 919 732 5196 Email info flexcellint com Issued April 2011 STREAMSOFT V4 2 SOFTWARE 29 OSCIFLOW USER MANUAL NOTICE The information in this document is subject to change without notice Flexcell International Corporation assumes no responsibility for any errors that may appear in this guide This manual is believed to be complete and accurate at the time of publication In no event shall Flexcell International Corporation be liable for incidental or consequential damages in connection with or arising from the use of this manual STREAMSOFT V4 2 SOFTWARE 30 OSCIFLOW USER MANUAL Contacting Flexcell North America Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 USA Phone 919 732 1591 800 728 371
29. ting Apparatus n 19 Configure System MAADILI HA IK een la di ence iiniee oe 21 Reimitialize Hardware A Weta 22 STREAMSOET V42 NOTES veg uevecvesseusoysscenycrecveseusburseverisvevetvergeypas RATEN TRATEN 23 DOING AN EXPERIMENT csccasscsscccossessesssccteccescssssesssesestaseiscsesssubiebuiatcesesoasscntessadescscshsodsdsssaacesesscasessbsceteseshockese sescsaasssabeisedece 24 CREATING A REGIME A sieeve debentaveerstebe 24 FILLING THE SYSTEM TO ELIMINATE AIR BUBBLES c sssesssssssssesesssseesseesesesssscecssesssssssseesesesesssseesssesesnsssscessseessseseansssseessseses 25 OSCIFLOW TROUBLESHOOTING dicas 26 WHEN THE OSCIFLOW VALVES ARE NOT RESPONDING scssscsssesssscssuccsuccssecssecssucssscsssscesscssucssnecssecssecssucssusstsesssussssesssecsseeeseee 20 WARRANTY INFORMA TION sisscciecocsevassticcecossceccscesosssececdessetsccsetsvosdesceoscesssedesdessesisesctesssdasdeossesesesscsessvasonsetsvoscesdecsbe cosedeoeeds 29 CONTACTING FLEXCELL sissccisi scciescicssdiscoseceiesecdesssosedecdeccotosncesevescescevssoceassesdessodececntesoagesdebsseeessencsccseessssescueseesdoosecotebeseete 31 STREAMSOFT V4 2 SOFTWARE 2 OSCIFLOW USER MANUAL Getting Started Introduction Fluid induced shear stress occurs in every tissue in the body as a result of interstitial fluid movement Tissue deformation by compression tension or shear forces results in the movement of interstitial fluid around cells Fluid movement acts as a transport ve
30. wave mode where the flow is stopped and then restarted after a designated period of time Software control through StreamSoft software allows the user to regulate frequency of oscillation or pulsatile patterns as well as shear stress levels applied to cells Individual protocols can be designed for flow experiments The frequency of oscillation or pulsation is specified by programming seconds ON and seconds OFF in the software An advantage that the OsciFlow has over other flow reversal techniques is that it overcomes inertial lag Other techniques for flow reversal rely on a change of pump motor rotation With this strategy the inherent inertial effects associated with the deceleration and acceleration creates an unavoidable response lag time As the OsciFlow does not require a pump motor to change directions near instantaneous flow reversal can be achieved with less wear and tear on pumps and motors STREAMSOFT V4 2 SOFTWARE 3 OSCIFLOW USER MANUAL OsciFlow System Specifications e Maximum Frequency of Oscillation 1Hz 0 5 sec on 0 5 sec off e Minimum Frequency of Oscillation No limit e Maximum Frequency of Pulsation 1Hz 0 5 sec on 0 5 sec off e Minimum Frequency of Pulsation No limit e Maximum Flow Rate through valves No Limit e Pinch Valve Maximum Pressure 20 psi e Pinch Valve Maximum Fluid Temperature 158F 70 C e Box Dimensions 13 x 7 5 x 7 5 33cm x 19 1cm x 19 1cm e
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