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EXO User Manual

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1. 1 Launch KOR software and click the Connections menu 2 Click Rescan button 3 Click Search Bluetooth button This may take up to 40 seconds and may require several attempts using the Refresh button 4 Select the device from the list and click Connect When the connection is complete KOR automatically displays the Dashboard screen with live data pg 44 3b Establish Bluetooth link Win XP Click the Bluetooth icon in the system tray lower right select Add a Bluetooth Device and complete the device wizard 2 Activate your sonde s Bluetooth 3 Check My device is setup and ready to be found then click Next 4 Locate the sonde name starts with YSI from the list and click Next If not found click Search Again Select Enter the device s pairing code and click Next Then enter the pairing code 9876 Click Next Windows assigns an outgoing comm port Select Finish on NO See step 3a to complete the connection in KOR 3c Establish Bluetooth link Win 7 1 Open the Windows Start menu and click Devices and Printers 2 Select Add a Device from the top of the screen 3 Locate the sonde name starts with YSI from the list and click Next 4 Select Enter the device s pairing code and click Next Then enter the pairing code 9876 Click Next 5 Windows will configure the device install drivers and assign a comm port YSE S
2. When using rechargeable nickel metal hydride NiMH batteries Estimated battery life is not available because NiMH batteries vary greatly in manufacturer capacity and discharge curves We recommend a NiMH D cell battery with a minimum rating of 10 000 milliamp hours that are fully charged each time they are used pg 106 Sonde Install or Replace EXO 2 Batteries EXO2 water quality sondes use four 4 D cell batteries as a power source Using alkaline batteries users can expect approximately 90 days of deployment from a fully loaded sonde that samples once every 15 minutes However deployment times may vary greatly depending on water temperature sampling rate sensor payload wiper frequency and brand of battery See battery life specification next page Do not use Ni Cad rechargeable batteries in the EXO2 sonde 1 Loosen battery cap Start with a clean and dry sonde Slide the sonde tool s smaller opening over the battery cap on top of the EXO2 top EXO2 Using the tool as a lever firmly turn the tool counterclockwise until the battery cap is loose 2 Remove battery cap and old batteries Once the cap is sufficiently loose remove the cap and old batteries from the well Always dispose of used alkaline batteries according to local requirements and regulations Clean the o ring sealing surfaces with a lint free cloth Inspect down into the battery tube to make sure it is clean and dry 3 Insert new batteries A Do not use 3
3. Altitude Latitude Enter the coordinates for the local altitude in feet relative to sea level and latitude in degrees where the sonde is sampling Latitude values are used in the calculation of depth or level to account for global variations in the gravitational field pg 85 Calibration pH point Select the 1 point option to calibrate the pH probe using one calibration standard 2 point Select the 2 point option to calibrate the pH probe using two calibration standards In this procedure the pH sensor is calibrated with a pH 7 buffer and a pH 10 or pH 4 buffer depending on your environmental water A 2 point calibration can save time versus a 3 point calibration if the pH of the media to be monitored is known to be either basic or acidic 3 point Select the 3 point option to calibrate the pH probe using three calibration standards In this procedure the pH sensor is calibrated with a pH 7 buffer and two additional buffers The 3 point calibration method assures maximum accuracy when the pH of the media to be monitored cannot be anticipated Review the basic calibration description in section 5 1 Pour the correct amount of pH buffer in a clean and dry or pre rinsed calibration cup Carefully immerse the probe end of the sonde into the solution making sure the sensor s glass bulb is in solution by at least 1 cm Allow at least 1 minute for temperature equilibration before proceeding In the Calibrate menu select
4. Specifications Temperature Operating Storage 5 to 50 C 20 to 80 C i Shallow O to 33 ft 10 m Medium O to 328 ft 100 m Deep O to 820 ft 250 m i Vented O to 33 ft 10 m i Shallow 0 04 FS 0 013 i ft or 0 004 m i Medium 0 04 FS 0 13 ft i or 0 04 m i Deep 0 04 FS 0 33 ft or ATON i Vented 0 03 FS 40 010 ft ior 0 003 m Sensor Type Stainless steel strain gauge pg 17 al T8 Location of Depth Sensor Depth sensors on the EXO2 sondes are not on center When deploying the sonde vertically take care to ensure the sonde is redeployed in same position Often a marker pin inside a PVC pipe is used In horizontal deployments take care to ensure the redeployments are always in the same orientation This is especially important for the EXO2 sonde because the depth sensor is off axis To assist with consistent horizontal orientation the EXO2 sonde has an indentation at the top of the sonde for a marker or positioning pin The sonde should be installed with at least 1 cm of water above the intake ports If a conductivity sensor is installed the depth will be compensated automatically for changes in the density of water as temperature and salinity change Depth Configuration EXO sondes must be ordered with a specific depth option 59950x 00 no depth 59950x 01 0 10 m depth 59950x 02 0 100 m depth 59950x 03 0 250 m depth 59950x 04 0 10 m ven
5. 11 28AM _ 733 2993 Bar mmHg e 7 points are not active grayed out gt y O Temperature This field displays current temperature if a Port 2 PH ORP conductivity temperature sensor is installed If no sensor is installed user can input data into this field Port 3 CT Port 4 Optical DO Barometer Displays for DO calibrations only Standard Value User input field for the calibration setpoint based on the value of the standard being used pg 66 Type Optional field for type of standard being used Pre populated for some calibrations user input field for other calibrations Optical DO ODO sat Manufacturer Optional field to record manufacturer of calibration standard used This data is logged in the calibration worksheet Lot number Optional field for calibration standard lot number used for traceability purposes Advanced calibration menu OSS er Each sensor calibration menu has an Advanced button to zow om edit advanced features for the specific parameter Unique gt sensor options include TSS input for Turbidity not available on Handheld when performing multi sensor calibration and sensor cap coefficients for DO Uncalibrate The Uncal function in the Advanced menu returns the probe back to factory calibration settings Users may select this for troubleshooting if a calibration process on a probe is not working correctly On the Handheld version there is a checkbox Restore Calibr
6. Connections menu Then select Rescan and select Confirm Locate your sonde in the menu and select Connect EXOwater com K 0 1 0 561 pg 40 Attach Sonde to Handheld Bluetooth Wireless Users can wirelessly connect EXO sondes above water to the EXO Handheld using Bluetooth wireless With Bluetooth users can reduce the amount of cables needed to operate their sonde This wireless connection has a typical range of 10 meters but this range will fluctuate depending on the operating environment Users cannot wirelessly connect through water In order to connect via wireless both devices must be powered on 1 Activate sonde s Bluetooth Activate Bluetooth by holding a magnet at the magnetic activation area NOTE There is a magnet in the top of the Handheld as well In addition to magnetic activation users can also activate Bluetooth by cycling power to the sonde remove reinstall batteries cs NOTE A new magnetic tool is available Item 599469 EXO Sensor Tool Kit magnet inside tool 2 Discover sonde in KOR Kor For the XC Platform Connecting to Wired Sonde comm _ K 0 1 0 561 733 8244 Bar mmHg Fii Detected Device s nottom LON700445 Every time the Handheld powers on it automatically searches for a sonde via the hard wired cable connection and will connect to the sonde if the Auto Discover option is enabled in the Connection Settings menu Rescan sonde
7. Flow cell volumes without sensors installed are approximately 410 mL for EXO1 and 925 mL for EXO2 1 Inspect sonde and flow cell Remove the sonde guard or calibration cup from the sonde so that the sensors are exposed Make sure that the threads of the sonde and flow cell as well as all o rings are clean and free of any particles such as sand grit or dirt 2 Insert sonde into flow cell A ie Insert the sonde into the top of the flow cell Be careful not to bump or scrape the sensors on the sides of the flow cell Screw the sonde into the flow cell by turning the sonde clockwise until it is hand tightened into place do not use a tool 3 Connect tubing to flow cell Install the Quick Connect tube fittings onto the flow cell by inserting them into the Quick Connect coupling body They should snap into place Connect the tubing from your pump not included to the Quick Connect tube fittings making sure that the tubing is Inflow pushed securely onto the fittings The inflow should be at the bottom of the flow cell and the outflow should be at the top Keep flow cell vertical to purge it and ensure air release from Conductivity Temperature sensor Do not turn on water to the system until the flow cell is securely connected pg 49 Connect Field Cables with Cable Coupler A cable coupler is required to join two EXO field cables together or a field cable to a flying lead cable The cable coupler is available in either
8. For most lifetime based optical DO sensors this Stern Volmer relationship Tzero T 1 versus O pressure is not strictly linear particularly at higher oxygen pressures and the data must be processed using analysis by continued Specifications Temperature i 5 to 50 C i 20 to 80 C i O to 500 air sat O to 50 mg L 0 200 1 reading or 1 i air sat whichever is greater 200 500 5 reading 0 20 mg L 1 of reading or 0 1 mg L i 20 50 mg L 5 reading Operating Storage 0 1 air sat 0 01 mg L Optical luminescence lifetime Sensor Type g 19 polynomial non linear regression Fortunately the non linearity does not change significantly with time so that as long as each sensor is characterized with regard to its response to changing oxygen pressure the curvature in the relationship does not affect the ability of the sensor to accurately measure oxygen for an extended period of time Variables that Affect DO Measurements Variables that could affect dissolved oxygen measurements include temperature salinity and barometric pressure Temperature and salinity are compensated for during instrument calibration and field use with the use of additional sensors and or instrument software settings Barometric pressure relates to the pressure of oxygen in the calibration environment and barometric pressure changes due to a change in altitude or local weather Generally the effect of b
9. Short term and Long term 6 2 Sonde Maintenance 6 3 Install Replace EXO1 Sonde Batteries and Battery Life 6 4 Install Replace EXO2 Sonde Batteries and Battery Life 6 5 Replace EXO1 Sonde Bails 6 6 Replace EXO2 Sonde Bails 6 7 Update Sonde Firmware 6 8 Handheld Maintenance and Storage 6 9 Install Replace Handheld Batteries 6 10 Update Handheld Firmware and KOR Software on Handheld 6 11 Update Sonde Firmware Using Handheld 6 12 Depth Sensor Storage and Maintenance 6 13 Standard Optical Sensors Maintenance and Storage 6 14 Conductivity Temperature Sensor Maintenance and Storage pg 3 Table of Contents Continued 6 15 Dissolved Oxygen Sensor Storage 6 16 DO Sensor Maintenance and Rehydration 6 17 DO Sensor Cap Replacement 6 18 pH and ORP Sensor Storage and Rehydration 6 19 pH ORP Maintenance 6 20 ISEs Ammonium Nitrate and Chloride Sensor Storage and Maintenance 6 21 Sensor Module Replacement 6 22 Wiper Maintenance and Storage 6 23 Field Cable Maintenance and Storage 6 24 Connectors Maintenance and Storage 6 25 Antifouling Equipment Maintenance 6 26 Flow Cell Maintenance 6 27 Storage Cases 7 Vented Level Sonde 7 1 Overview 7 2 Vented Level Sonde Installation 7 3 Cables and Desiccants Installation 7 4 Calibration 7 5 Maintenance and Storage 8 Health and Safety Warranty Service 8 1 Chemicals Ammonium and Nitrate Solutions Conductivity Solutions pH Solutions Zobell Solution Turbidity Sta
10. Software menus and features are subject to change NOTE This feature was introduced in the February 2014 software update If not available on your software please visit EXOwater com to get the latest upgrades Add a Site Click Add to create and name a site FOna Bw YO Aad Remove Sites are displayed in the order they were created Remove a Site To remove a site highlight an existing entry and click Remove Sync Sites with Handheld For instructions on syncing your site list with the Handheld reference the instructions in section 3 8 pg 71 KOR Software Data Menu The Data menu is used to transfer files from the sonde or handheld and manage data files on a local computer The Transfer function will only work when connected to a sonde Transfer Clicking the Transfer submenu button initiates a scan of the attached EXO and lists all files on the sonde Upload copy files Select files to upload by clicking a file name in the list clicking the Select All button or clicking multiple files by holding down the Control key Selected files are highlighted in blue After files are selected use the Selected button to copy the file to the PC Click the Latest button to copy the most recent file The uploaded file s are in binary format and are stored in the Program Files KOR EXO Data Files folder on the computer See Data Files amp File Locations section 4 10 for more detail Delete files After f
11. The retaining screws are captured into this panel and are not independently removable If replacement is necessary replace the entire assembly Insert the Li lon battery pack Remove existing batteries if necessary WARNING Be sure to remove the clear plastic tape from the back of the battery pack before inserting Failure to do so can cause damage to your equipment Install the pack by fitting it correctly into the battery compartment Ensure that the rubber battery cover gasket is seated properly then tighten the four retaining screws back into their holes CAUTION Overtightening of the screws is likely to cause damage and require replacement of the battery pack Prior to first use charge the Li Ion battery pack with the approved YSI charger See the FAQ s for more details Battery Life Rechargeable Li lon Battery Pack Specifications Dependent upon variables such as temperature screen brightness and feature use Up to 9 hours under active use for a new Lion battery pack Performance can be expected to decline over time and use Charging Operating Storage 2 C to 45 C 20 C to 60 C OG te 35G 35 6 F to 113 F A F to 140 F 32 F to 95 F Charging Supply Input Charging Supply Output 100 240V 12VDC 2 5A For accurate battery gauge use KOR Interface Software Operating System 1 0 2 Battery Pack Handling For information on how to handle a hot or damaged battery please reference the suppl
12. These differences are thought to be a result of differing optical components and geometries and the resulting detection of varying suspended sediment characteristics This effect is inherent in the nature of every turbidity sensor and as a result readings between different model turbidity sensors are likely to show different field values even after calibration in the same standards For long term in situ continuous monitoring of turbidity the EXO2 sonde has a wiper to clean the turbidity sensor to avoid sensor fouling and maintain accuracy og 130 EZB Install Batteries The EXO1 Sonde uses two 2 D cell alkaline batteries and the EXO2 Sonde uses four 4 D cell alkaline batteries as the recommended power source Alternatively the sonde may use rechargeable 1 5V high capacity NiMH D cell batteries that you purchase Do not use Ni Cad or Lithium batteries in the EXO sonde For detailed installation instructions please see section 6 3 for EXO1 and 6 4 for EXO2 A Do not use 3 6V Li batteries in the sondes Damage to the circuit board is not covered under warranty 1 Remove battery cover EXO1 Twist the blue battery cover counterclockwise to loosen lift up to remove Use included wrench to loosen if necessary A Do not remove the screws on the sonde s electronics compartment EXO2 Unscrew and remove battery cap Use included wrench to loosen if necessary 2 Install batteries EXO1 Lift up the rubber flaps not shown
13. Transfer this standard to a glass bottle and retain it for future use Accurately transfer 5 0 mL of the solution prepared in the above step to a 1000 mL volumetric flask and then fill the flask to the volumetric mark with deionized or distilled water Mix well to obtain a solution which is 0 625 mg L in water a 200 1 dilution of the concentrated solution For BGA PE calibration accurately transfer 0 2 mL of the 125 mg L solution prepared in step 2 to a 1000 mL volumetric flask and then fill the flask to the volumetric mark with deionized or distilled water Mix well to obtain a solution that is 25 ug L or 0 025 mg L of Rhodamine WT Store the concentrated standard solution in a glass bottle in a refrigerator to retard decomposition The dilute standard prepared in the previous step should be used within 24 hours of its preparation Discard the used standard When Rhodamine standards are required in the future perform another dilution of the concentrated Rhodamine WT solution after warming it to ambient temperature pg 100 Effect of temperature on fluorescence The intensity of the fluorescence of many dyes shows an inverse relationship with temperature This effect must be accounted for when calibrating the EXO Total Algae sensor with Rhodamine WT Enter the ug L or RFU value from the table below that corresponds to the temperature of the standard RFU i pg L RFU pg L C RFU Chl g L Chl BGA PC BGA PC BGA PE BGA PE Chloride
14. and users are expected to understand and follow the battery disposal requirements for their specific locale The circuit board in these instruments may contain a manganese dioxide lithium coin cell battery that must be in place for continuity of power to memory devices on the board This battery is not user serviceable or replaceable When appropriate an authorized service center will remove this battery and properly dispose of it per service and repair policies Rechargeable Li Battery Pack 1 When the battery is worn out insulate the terminals with adhesive tape or similar materials before disposal 2 Dispose of batteries in the manner required by your city county state or country For details on recycling lithium ion batteries please contact a government recycling agency your waste disposal service or visit reputable online recycling sources such as www batteryrecycling com This product must not be disposed of with other waste Instead it is the user s responsibility to dispose of their waste equipment by handing it over to a designated collection point for the recycling of waste electrical and electronic equipment The separate collection and recycling of your waste equipment at the time of disposal will help to conserve natural resources and ensure that it is recycled in a manner that protects human health and the environment For more information about where you can drop off your waste equipment for recycling please con
15. or an additional sensor and an auxiliary port on top of the sonde This auxiliary port will allow the user to connect the EXO2 to other EXO sondes See section 7 for information specific to vented level sondes e e Users communicate with the sonde via a field cable to an e EXO Handheld Bluetooth wireless connection to a PC or e EXO Handheld or a USB connection via communications e adapter to a PC e e Specifications e Operating Environment Depth Rating 250 meters 820 feet We Peter ere re eee errr rere A Xenoy Lexan bronze e titanium 316 stainless e ee err aaa 3 Memory Capacity Pressure Transducer e emery erica p hoer ly i NAE PE O P NNA E ENR E NAE AS e i Openings e e E oa e Communications Bluetooth Field Cable Wiper Sensor Port e Sonde USB RS 485 USB SDI 12 RS 232 aes Universal Sensor Ports 0 ee e e NOTE Starting early 2014 EXO sonde 4 D size batteries bulkhead material changed from bronze e T ae t a Ame PUNE nae ed Tae Pu uvege ere to titanium emperarure Operating 3 to 450 C Oe ee e Poco Ocoee Coote Cone CeCe Oo Oc ec Oe ooo eee Dimensions e Diameter 7 62 cm 3 00 in e length 71 1 cm 28 00 in Weight 3 60 kg 7 90 b w batt e pg 7 EXO2 Sonde 599502 xx Removable Bail 599474 Auxiliary Port gt 6 Pin Cable Connector Battery Cap Pressure Relief Valve Oring kit 599681 Battery Comp
16. the Bluetooth is enabled but no link has been established When the blue LED blinks at 2 Hz the sonde s Bluetooth is on and has established a link When the red sonde state LED is off the sonde is either Off or Asleep and not logging When it blinks at 0 1 Hz once every 10 seconds the sonde is Asleep and logging is enabled When the red light blinks at 1 Hz the sonde is Awake and has no faults If the red light is lit continuously the sonde is Awake and has detected faults such as problems with the system that need to be fixed prior to use Modes Within the Awake state the sonde has three modes which are activated via Kor software When Inactive Off the sonde does not log any data In Real Time mode the sonde continuously collects data at a user specified interval default is 2 Hz Sample Hold mode allows users to easily synchronize data between the sonde s data logger and an external data collection platform Awaken Sonde Activate Bluetooth Once power is applied to the sonde internally or externally users can awaken their sondes from Sleep state using any of several methods Primarily users activate EXO sondes and the Bluetooth connections via a magnetic switch installed in sonde s electronics compartment The sonde will automatically disable the connection and go to sleep once it has not received a Bluetooth signal for 5 minutes or a signal from the topside connector for 30 seconds In order to ac
17. the sensor you are going to calibrate from the list Next select the parameter for the sensor you are going to calibrate Some sensors have only one parameter option while other sensors have multiple options In the next menu select a 1 2 or 3 point calibration depending LPS SA TANTA HS on your sensor Enter the value of the standard you are using Check that the value you enter is correct and its units match the units at the top of the menu e g microSiemens versus milliSiemens You may also enter optional information for type of standard manufacturer of standard and lot number Click the Start Calibration button This action initiates the probe s calibration in the standard initially the data reported will be unstable and then they will move to stable readings Click the Graph Data button to compare the pre cal and post cal values in graph form Users should confirm that the value is within their acceptable margin of error Once readings are stable click Apply to accept this calibration point Repeat the process for each calibration point Click Complete when all points have been calibrated A calibration summary appears with a QC score View export and or print the calibration worksheet If a calibration error appears repeat the calibration procedure pg 81 Calibration Conductivity Temperature Clean the conductivity cell with the supplied soft brush before calibrating see section 6 14 Also review the basic cali
18. www EXOwater com return When service is required either at the user s facility or at the manufacturer the following steps must be taken to insure the safety of our service personnel e Inamanner appropriate to each device decontaminate all exposed surfaces including any containers 70 isopropyl alcohol or a solution of 1 4 cup bleach to 1 gallon tap water are suitable for most disinfecting Instruments used with wastewater may be disinfected with 5 Lysol if this is more convenient to the user e The user shall take normal precautions to prevent radioactive contamination and must use appropriate decontamination procedures should exposure occur e Ifexposure has occurred the customer must certify that decontamination has been accomplished and that no radioactivity is detectable by survey equipment e Cleaning must be completed and certified on any product before returning Packing Instructions e Clean and decontaminate items to insure the safety of the handler e Complete and include the Product Return Form found online e Place the product in a plastic bag to keep out dirt and packing material e Usea large carton preferably the original and surround the product completely with packing material pg 154 Instrument Service Recycling Batteries The user must remove and dispose of alkaline batteries when they no longer power the EXO1 sonde EXO2 sonde or EXO Handheld Disposal requirements vary by country and region
19. 6 1 3 Long term storage Users should remove the sensors from the sonde and place them in their storage bottle installed on sensor during shipping with a small amount of tap water or calibration standard The sensors should not be immersed in water A Do not store the ISE sensors in conductivity standard pH buffer salt water or any solution with significant conductivity ag l 126 4 Rehydrate reference junction If an ISE sensor has been allowed to dry soak the sensor for several hours preferably overnight in the sensor s high calibration solution If the sensor is irreparably damaged users must replace the sensor module ogi 127 6 21 Sensor Module Replacement EXO pH pH ORP ammonium nitrate and chloride sensors feature replaceable sensor modules 599795 599797 599743 01 599744 01 599745 01 due to the electrolyte depleting characteristics necessary to make such measurements We recommend that users replace these modules as necessary typically 12 to 18 months for pH and ORP and three to six months for ISEs if stored properly in a humid environment wetting the sponge in the provided bottle when not in use Working life will depend on the conditions of the deployment environment Perform this procedure in a clean dry laboratory environment 1 Remove old sticker and plug Peel off and discard the old sticker that covers the junction of the sensor body and the module Then with a small flat blade scr
20. ELIE Songe SN TiNsoz202 Sensor Firmware Version 103 SpCoed pSiem Sonde 10 tinsozzce f Sonde Firmware Version To Sonde Firmware Version 04 112 Calibration Parameter 000 sat Sone 0 Transaz202 Calibration Parameter v4 QC Score Cal Point 1 Col Point 2 Cal Point 3 Standard 1 10000 00 jsSiem 10032 00 pSicm Post Calibration Value 1000 00 pSiem Raw Vaise Cond RAW pSiom 904225 Temperature 2180 C A QC Score Cal Pont 1 Standard Ar Saturated Pre Calbraton Vaive S247 sat Post Calibration Value Sa 74 sat Raw Valve 000 RAW s Temperature ECHO Addtonsl put Baro mmg 780 00 Pre Caltraton Value Additional input 1 NIA Additional input 2 NIA Additional input 3 NIA Additional put 2 NIA Additional put 3 WA Type Type kel Manufacturer Manufacturer Ysi Cot Number Lot Number Tiirorsas Calibration Pont Accepted Calibration Pont Accepled YES Stab ty Achieved Stability Achieved YES Completed Ad ticea Post Calmration nto Apps Membrane SN 11L101151 Membrane into Last Updated 5 30 2012 18 41 47 000 Gam 1 07 Completes YES Applied les Vals T YES Sensor Removed tno Uncalibrated NO Additional Post Calibration Info Cell Constant 5 68 Vaii Sensor Removed Uncalibrated Marcware KOR Vi Hardware EXO Desitop phain Worksheet Version KOR Version 100 646 Worksheet Version Ti Additional Post Calibration Info ODO Gain The ODO gain
21. Handheld Systems Model numbers 599501 xx 599511 xx 599502 xx 599512 xx 599150 Accessories Sensors 599090 xx 599100 xx 599101 xx 599102 xx 599104 xx 599118 xx 599800 599810 599870 xx 599040 xx 599008 xx EXOISEOx Intentional Radiators EXO Sondes EXO1 and EXO2 contain the LMX Bluetooth module Nemko certified body ID CE 2302 C Tick number N136 EXO Handheld 599150 contains a Wi Fi Bluetooth module Nemko certified body ID CE 2302 C Tick number N136 Directives e EMC 2004 108 EC e Australian ACMA Standards for C Tick mark Section 182 of the Radiocommunications Act 1992 e New Zealand RSM Standards Radiocommunications Act 1992 e Telecommunications Labeling Notice 2001 under section 407 of the Australian Telecommunications Act 1997 Standards e EN61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General Requirements e ACMA Radio Communications Short Range Devices 2004 e AS NZ 4268 2008 e Radio Communications Electromagnetic Radiation Human Exposure Standard March 2003 VILLE Lisa M Abel Director Quality June 7 2012 pg 148 The undersigned hereby declares that the products listed below conform with all applicable requirements of the Radio Waves Act of Korea for intentional radiators Manufacturer YSI Incorporated a Xylem brand 1725 Brannum Lane Yellow Springs OH 45387 USA Equipment name EXO Sondes EXO1 and EXO2
22. LIABLE FOR ANY SPECIAL INDIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY pg 153 e 8 5 Instrument Service Cleaning and Packing EXO Authorized Service Centers are located in the United States and around the world Please refer to the EXO website EXOwater com for your nearest Authorized Service Center Cleaning Instructions Before they can be serviced equipment exposed to biological radioactive or toxic materials must be cleaned and disinfected Biological contamination is presumed for any instrument probe or other device that has been used with body fluids or tissues or with wastewater Radioactive contamination is presumed for any instrument probe or other device that has been used near any radioactive source Product Return Form Find the product return form online www EXOwater com return Cleaning Certificate If an instrument probe or other part is returned or presented for service without a Cleaning Certificate and ifin our opinion it represents a potential biological or radioactive hazard our service personnel reserve the right to withhold service until appropriate cleaning decontamination and certification has been completed We will contact the sender for instructions as to the disposition of the equipment Disposition costs will be the responsibility of the sender Find the cleaning certificate on the back of the online product return form
23. Language gt Reboot NOTE Changing the language of the Handheld requires a restart to take effect Power Sonde Settings gt Handheld gt Power Sonde Turn On or Off the ability for the Handheld battery to power the sonde A WARNING Powering the sonde via the Handheld is not recommended for typical use Due to the rapid drain on the Handheld batteries in this mode it is suggested to only use this mode for short bursts Sound Volume Settings gt Handheld gt Sound Volume Allows the user to select the volume of the Handheld speaker including the ability to turn it off completely Hibernate Settings gt Handheld gt Hibernate min Allows the user to select how much time in minutes min the Handheld will wait without input before going into a power saving sleep mode NOTE Selecting zero will keep the Handheld on continuously Time Display Mode Settings gt Handheld gt Time Display Mode Allows the user to adjust the Handheld clock display to the selected time zone A universal time zone option is also available Adjust Date Time Settings gt Handheld gt Top Left Button Allows the user to change specific parameters of the time display pg G2 KOR Software Introduction Users interface with the EXO sondes and handheld via KOR software Once the software is installed and a device is connected to a computer or an EXO handheld launch the KOR software For KOR Installation instructions see section 2 11 Na
24. Points Enter 0 or go to the Advanced menu to enter a known sensor offset Ifa depth offset is entered the output value will shift by the value of the offset Users may use an offset if referencing a water elevation against a known datum Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point This process zeros the depth sensor Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu For best performance of vented level measurements users should ensure that the orientation of the sonde remains constant while taking readings Keep the sonde still and in one position while calibrating Advanced Mounting Use the Advanced menu to select if a sonde will be mounted in a moving profiling deployment instead of a fixed location Depth Offset Enter a positive or negative depth offset value if the sonde has been surveyed into a geodetic reference Altitude Latitude Enter the coordinates for the local altitude in meters relative to sea level and latitude in degrees where the sonde is sampling Latitude values are used in the calculation of depth and level to account for global variations in the gravitational field NOTE You must be within 500 feet and 1 degree respectively Maintenance and Storage Short ter
25. Soak in HCl solution Soak the sensor for 30 60 minutes in one molar 1 M hydrochloric acid HCl This reagent can be purchased from most distributors Following the HCl soak rinse the sensor in clean tap water and allow it to soak for an hour in clean water Stir the water occasionally Then rinse the sensor again in tape water and test response time If response time does not improve or you suspect biological contamination of the reference junction continue to the next soak If HCI is not available soak in white vinegar AN Follow the HCI manufacturer s instructions carefully to avoid personal harm 3 Soak in chlorine bleach solution Soak the sensor for approximately one hour in a 1 1 dilution of chlorine bleach and tap water Following the soak rinse the sensor in clean tap water and allow it to soak for at least one hour in clean water longer if possible Then rinse the sensor again in tap water and test response time Chlorine Bleach pg 125 6 20 ISE Sensors Maintenance and Storage EXO ammonium nitrate and chloride sensors utilize ion selective electrodes ISEs to monitor these parameters One key requirement of storage short or long term for these sensors is their reference electrode junctions should never dry out This section will identify storage as long term or short term Long term denotes storage during times of long inactivity over wintering end of monitoring season etc Short
26. TELEC certificate number 001 A00577 Exo2 TELEC certificate number 001 A00578 EXO Handheld contains a Wi Fi Bluetooth module certificate number 001 A00579 Regulations TELEC Article 38 24 Paragraph 1 of the Radio Law PATON Lisa M Abel Director Quality June 7 2012 pg 151 The undersigned hereby declares that the products listed below conform with all applicable requirements of the Anatel Regulations of Brazil for intentional radiators Manufacturer Equipment name Model numbers Intentional Radiators Regulations hill YSI Incorporated a Xylem brand 1725 Brannum Lane Yellow Springs OH 45387 USA EXO Sondes EXO1 and EXO2 and EXO Handheld Systems 599501 xx 599511 xx 599502 xx 599512 xx 599150 Intentional Radiators EXO Sondes EXO1 and EXO2 contain the LMX Bluetooth module Certificate of Homologation No 0657 13 8838 Certificate of Conformity No 07473 13 EXO Handheld 599150 contains a Wi Fi Bluetooth module Certificate of Homologation No 1281 13 8838 Certificate of Conformity No 07769 13 Anatel Transceptor de Radiacao Restrita Categoria II Lisa M Abel Director Quality June 7 2012 pg 152 8 4 Instrument Warranty Warranted against defects in workmanship and materials when used for their intended purposes and maintained according to instructions and exclusive of batteries and any damage caused by defective batteries Warranty Card Register your preaucl wate Two
27. a surrogate for CDOM N WARNING UV LIGHT Do not look directly 599104 01 at end of sensor Quinine Sulfate A surrogate for fDOM is Quinine Sulfate which in acid solution fluoresces similarly to dissolved organic matter The units of DOM are quinine sulfate units QSUs where 1 QSU 1 ppb quinine sulfate and thus quinine sulfate is really a double surrogate for the desired CDOM parameter The EXO fDOM sensor shows virtually perfect linearity R 1 0000 on serial dilution of a colorless solution of quinine sulfate However on serial dilution of stained water field samples the sensor shows some underlinearity The point of underlinearity in field samples varies and is continued Specifications Temperature Operating i 5 to 50 C 20 to 80 C R gt 0 999 for serial dilution of 300 ppb Quinine Sulfate solution i Optics Excitation 3655 nm Emission 480 40 nm affected by the UV absorbance of the DOM in the water Testing shows that underlinearity can occur at DOM concentrations as low as 50 QSU This factor means that a field sample with an DOM reading of 140 QSU will contain significantly more than double the fDOM of a sample that reads 70 QSU This effect good linearity in colorless quinine sulfate solution but underlinearity in stained field samples is also exhibited by other commercially available fDOM sensors and thus the performance of the EXO sensor is likely to be equivalent or be
28. average with older data points to create a smooth curve If the new reading is significantly different than past measurements then it restarts the rolling average of data points Averaging options Default This mode provides optimum data filtering for all EES EE sensors Provides the highest accuracy automatic averaging T during unattended monitoring or fixed mooring This mode has DEE mm up to 40 seconds of filtering on the sensors NOTE This is the mode all sensors ship in and how sensors Check Sonde Deploy Time lt gt filtered data prior to this update E Default Sonde Date Time 9 20 2013 11 01 05 UTC Accelerated This mode should be used for spot sampling and slow or paused depth profiles The sensors are averaging 5 10 seconds of data in a rolling window unless there are any outliers eFOdR Be O Rapid This mode should be used where the sonde is moving quickly through the water such as with rapid profiling and unique applications like auvs gliders or towed applications The data Chattahooche River Site 2 will be noisy and will never settle on a single steady number This wou mode has 2 3 second filtering on the sensors CECR unam bast ch sonan pnt Tae Zone W hope Stantert Trew STC att OO Amatersam Berm Bem Rone Boanam veme TIP Enable the Vertical Position parameter in the Depth unit options to view the real time position of the sonde in the water column This is helpful in profilin
29. calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde RFU 1 or 2 point RFU is a percent full scale output it outputs relative fluorescence from 0 100 This calibration procedure is recommended if you are also using grab samples to post calibrate in vivo algae readings Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water In the Calibrate menu select BGA PC Chlor then select BGA RFU Select either a 1 or 2 point calibration Enter 0 for first standard value and 16 for second standard value Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibra
30. eagle G9 i LENE GSES UNEASE EERE AUG sae Conductivity lt 1500 pS cm continued Specifications continued Chloride Cl mg L Cl millivolts Temperature Operating Storage 15 of reading or 5 img LCl whichever is greater Sensor Type lonrselective electrode Salinity NOTE Qualification testing for chloride was performed in a stirred calibration solution Due to the solid state nature of the chloride ISE the sensor exhibits moderate flow dependence Mitigation can be achieved by stirring during calibration The chloride sensor uses a solid state membrane attached to a conductive wire This sensor operates in a similar fashion to the ammonium and nitrate sensors For all ISEs the linear relationship between the logarithm of the ammonium nitrate or chloride activity and the observed voltage as predicted by the Nernst equation is the basis for the determination Ammonium is calculated from the pH salinity and temperature readings If a pH sensor is not in use the instrument will assume the sample is neutral pH 7 for the calculation If a conductivity sensor salinity is not in use the instrument will use the salinity correction value entered in the ammonium sensor calibration screen for the calculation Replaceable Sensor Module The EXO ammonium chloride and nitrate sensors have a unique design that incorporates a user replaceable sensor tip module and a reusable sensor base th
31. in Zobell solution or DI water 3 Rehydrate reference junction If the pH sensor has been allowed to dry soak the sensor for several hours preferably overnight in a 2 molar 2 M solution of potassium chloride KCl In order to create a 2 M KCI solution dissolve 74 6 g of KCl in 500 mL of distilled or deionized water If KCI is unavailable a tap water or pH 4 buffer soak may restore function If the sensor is irreparably damaged users must replace the sensor module oy 124 pH and pH ORP Sensors Maintenance pH and pH ORP sensors will require occasional maintenance to clear contamination from the sensing elements These contaminants can slow the sensor s response time Clean the sensors whenever deposits biofouling or other contamination appear on the glass or when the sensor s response time slows perceptibly Remove the sensor from the sonde before performing the following cleaning steps Do not attempt to physically scrub or swab the glass bulbs The bulbs are very fragile and will break if pressed with sufficient force Maintain connectors as instructed in section 6 24 Replace depleted sensor module as instructed in section 6 21 1 Soak in dishwashing liquid solution Soak the sensor for 10 15 minutes in a solution of clean water and a few drops of dishwashing liquid Following the soak rinse the sensor with clean water and inspect If contaminants remain or response time does not improve continue to the HCl soak 2
32. is a diagnostic value recorded on the Calibration Worksheet and used for advanced diagnostic purposes The nominal value is 1 and accurate calibrations of the DO sensor will only slightly deviate from this number Cell Constant The cell constant is the current value of the conductivity and is a function of the factory original cell constant and the most recent user calibration The cell constant will drift over time based on the sensor s electrodes and the cell constant can be used to track drift Slope The slope for the pH sensor is the mV per decade pH unit where 59 is the typical value Slope allows the user to track drift away from 59 to determine the life aging of the sensor module Change mV The change millivolts is the delta mV change between either 4 and 7 or 7 and 10 calibration values for the pH sensor It is the mV deviation away from the middle calibration point number pg 102 Sonde Storage Proper sonde storage helps to ensure proper sonde operation To keep sondes in their best working order users must follow these instructions This section will identify storage as long term or short term Long term denotes storage during times of long inactivity over winter end of monitoring season etc Short term denotes storage during times the sonde will be used at a regular interval daily weekly biweekly etc 1 Short term storage For interim storage users should keep sensors moist but not submerged su
33. longer cables are made to order and there is an eight week lead time to build the product There are some limitations for applications using EXO cable lengths greater than 100 meters whether by extended cables or by means of cable coupling N To prevent system problems related to power and signal integrity make sure you understand the system limitations if you plan to use cable couplers or extended cables Voltage drop through long cables can adversely affect the available power at the sonde Here are some techniques to prevent such problems e Use Alkaline or high capacity NiMH batteries in the sonde This serves a dual purpose of adding weight in the sonde for profiling applications as well as preventing system reboots during period of high current demand Do not use EXO s USB SOA or Handheld as the sole power source for systems with large payloads many optical or high power sensors These devices do not provide a voltage high enough for use with extended cables eLimit use of EXO s auxiliary port to lower power devices ePower the sondes with a regulated power supply 12V 14V capable of supplying 1A This will ensure sufficient power is reaching the sonde a l12 EXO Sensors Overview The EXO product line includes nine sensors that detect a variety of physical chemical and biological properties of natural water EXO sensors are designed to collect highly accurate data under ever changing environmental conditions D
34. of air saturation is likely to compromise the accuracy specification of the EXO sensor For highest accuracy calibrate in saturation In the Calibrate menu select ODO then select ODO mg L Calibrating in ODO mg L automatically calibrates ODO sat and vice versa Click 1 Point for Calibration Points Enter the known mg L concentration for the standard value Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete Rinse the sonde and sensor s in tap or purified water and dry pg 83 ODO sat ODO local or mg L 2 point or zero point Normally it is not necessary to perform a 2 point calibration for the DO sensor and the procedure is not recommended unless a you are certain that the sensor does not meet your accuracy requirements at low DO levels and b you are operating under conditions where you are certain to be able to generate a medium which is truly oxygen free For ODO sat or ODO local calibrate your sonde at zero oxygen and in water saturated air or air saturated water For ODO mg L calibrate your sonde at zero oxygen and a known concentration of oxygen within 10 of air saturation The key to performing a 2 point calibration is to make certain that your zero oxygen medium is truly oxygen free If you use nitrogen gas for the zer
35. the 10 mg L standard to a storage bottle pg 101 Calibration Calibration Worksheet The Calibration Worksheet is a record of the calibration for an EXO sensor The worksheet contains quality assurance information including date and time of calibration date of previous calibration sensor firmware version type of calibration performed standard used and QC score Calibration Worksheets are saved in the Calibration Files folder on the computer not on the sonde All saved Worksheets can be accessed and viewed through the Data menu in KOR software Sample VVorksheets 1 point calibration of specific conductance on EXO conductivity temperature probe Calibration Worksheet Conductivity Temp 114990123 1 point calibration of percent saturation on EXO optical dissolved oxygen probe Calibration Worksheet Optica DO 11101477 Coordinated Universal Tene ersaot2 024134 PM osamiz 024252 PM onzo 1200 00 au UTC Time Coordinated Universal Time UTC Time Teso 0224 38 PM Veusra0r2 0224 38 PM Start Date Time 652012 02 4134 Pu 6 5 2012 02 31 41 PM 61512012 0231 41 PM End DatesTime i USONE 024252 Pal Terao 12 00 00 AM Terot2 12 00 00 AM Previous Calibration Date Time E02 120000 AM Start Date Time End Date Time Previous Calibration Date Time Sensor Type Optical DO Sonde Type EX02 Sonde Sensor SN Tusiorer Sonde SN Tviwsez202 Sensor Frmware Version 102 E Sensor Type Conductiity Temp Sonde Type EXO2 Sonde Sensor SN
36. the screen See more detail section 3 7 57 0 2 4 TOS mgA 1 Turb TSS mgA 4 Sonde Batt V 0 SEAN Gene Dashboard image at left live graph image at middle left 7 70 0 61 PH 2 Chlor ug G 1 26AM 733 6744 Bar mmHg ii Parameter preference TDS majl 3 Users can select which parameters to display in graph 1320 mode by using the backspace and tab keys on the Handheld keypad If an expected parameter is not available first set the appropriate units preference from the Options Units menu 11 25 50 AM 11 26 10 AM 11 26 30 AM faisaim 7336744 Bar mmg D P Advanced display settings iad Adjust data display settings for both dashboard and live Graph Type Time Series graph modes in the Run menu Select Settings to change Defaut View Dashboard the settings for graph type time series or vertical profile Default Param A e TempC gt sample interval default is 1 sec and default parameters Default Param B to view Sample Interval sec pg 57 3 7 Collectin with Handheld Data Collecting sampling data on the EXO Handheld is easy due to the array of logging options available via the Capture Data menu Run gt Dashboard gt Capture Data 44 49 Chattahooche River D F amp 1 Log 1 point to current file 2 Start logg ng with current file 3 Log 1 point to new file 4 Start logging with new file S Change current file 6 Change active site Current Logging File lt Not Set gt Ac
37. titanium 599430 01 for deployment or brass 599430 02 for laboratory and non submerged use Review these instructions for the proper installation of the cable coupler 1 Position and install coupler on first cable Prior mating the cables together install the coupler on the first cable Pull the black retaining collar away from the male 6 pin connector and then install the coupler onto the exposed cable between the collar and the connector teese A Align the coupler in the correct orientation with the smaller interior diameter positioned toward the black collar Incorrect orientation of the coupler does not allow it to slide over the connector later Screw the black retaining collar onto the end of the coupler 2 Connect cables Apply small dabs of Krytox grease to the sockets of the connector on the second cable Then align the 6 pin connector of the first cable with the sockets of the second cable and press them firmly together so that no gap remains 3 Connect coupler to second cable Slide the black collar and coupler toward the joined connectors Slide the white retaining collar of the second cable to the coupler and twist to securely attach the two components together Attach the strain reliefs of each cable together via the carabiners pg 50 Handheld Install or Replace Batteries The EXO Handheld HH uses four 4 C cell alkaline batteries as a power source Alternatively a rechargeable Li Ion batter
38. years cables sondes bulkheads handheld conductivity temperature depth and optical sensors electronics base for pH pH ORP ammonium chloride and nitrate sensors and accessories online warranty card www EXOwater com warranty One year optical DO membranes and replaceable reagent modules for pH and pH ORP Three months replaceable reagent modules for ammonium chloride and nitrate Regular maintenance of sondes and sensors such as replacing damaged o rings is described in the Maintenance section of this manual Users are expected to follow these guidelines to keep their equipment in good and proper working order and to protect the warranty on the product Damage due to accidents misuse tampering or failure to perform prescribed maintenance is not covered This warranty does not include batteries or damage resulting from defective batteries As documented in the Maintenance section of this manual batteries should be removed from all sondes and handheld when the product is not in use Since many battery manufacturers will repair or replace any equipment that has been damaged by their batteries it is essential that leaky or defective batteries be retained with the damaged product until the manufacturer has evaluated the claim The warranty period for chemicals and reagents is determined by the expiration date printed on their labels Within the warranty period we will repair or replace at our sole discretion free of
39. 232 output and power source into the other Mount the SOA in a humidity controlled enclosure using the following recommended hardware and never attempt to perform electrical work beyond your experience 1 Configure sonde Using KOR software go to the Deploy menu and choose to deploy by opening a template or starting a custom deployment Click Edit then go to the SDI 12 tab and select your parameters and the SDI address The sonde s default address is zero 0 Click to save with one of these options Save deploy start logging Save deploy no logging Save only Refer to section 4 4 KOR Software for more details Alternatively you can watch KOR Software orientation videos on our You Tube channel YouTube com YSlinc 2 Route cable Determine the sonde cable routing to the DCP and protect the cable from chafe damage and impact Route the cable through a sealing gland into the DCP and ensure that the seal is air tight and water tight 3 Mount SOA Users can mount the SOA horizontally or vertically either by the screw slots on the sides of the SOA or with the included 3M adhesive strips The screw slots were designed to accept 4 wood screws but may accept other types and sizes If the user opts to use adhesive strips first clean the application surface with a 50 50 mixture of isopropyl alcohol and water then mount them in temperatures between 21 and 38 C 70 and 100 F and allow the bond to cure for 72 hou
40. 6V Li batteries in the sondes Damage to the circuit board is not covered under warranty With the positive terminal facing up insert four 4 new D cell batteries into the battery well pg 107 4 Check and service o rings A Before replacing the battery cover inspect and service the four o rings Ensure that the o rings are not nicked or torn and that they have no contaminants or particles on them or the sealing surfaces inside the battery cover Then apply a thin coat of Krytox lubricant to each o ring and sealing surface 5 Replace battery cap After servicing the cap s o rings insert the cap in its recess Then using your thumb press down on the pressure relief valve while turning the cap clockwise Once the cap threads are engaged use the tool to tighten until snug Do not overtighten overtightening will not create a strong seal and may damage the sonde When completed the top o ring of the cap must be below the battery compartment opening If a battery failure occurs that results in battery acid leakage into the battery compartment the sonde must be returned to a service center for evaluation Some battery acid will damage the plastic in the battery compartment Pressure in Battery Compartment The EXO2 sonde is equipped with a pressure relief valve to protect against catastrophic battery failure If the valve is open indicating an over pressure situation the battery cap must be replaced Significant water
41. C ens 11 9 cm 4 7 in 22 9 cm 9 0 in 0 91 kg 2 1 Ib pg 9 EXO Handheld 599150 peeeeeeessesoccececesesesooeeeeeeseseooeeeeeesssooceeeeee Bluetooth Indicator Daylight viewable LCD Escape Navigation Arrows 4 amp Return Backspace Tab Power Brightness Alphanumeric Keypad sa 10 EXO Cables Overview The EXO rugged field cable comes in many different lengths and options to meet the needs of your specific application Selecting the correct cable length and coupler will ensure the best quality data for your project For a full list of cable options and precautions for extended cables please see the following page Strain Relief Carabiner 2 Female white wet mate Rugged field cable connector Supports 60 pounds of gt longterm weight and up to 250 pounds for brief periods Male black eae er wet mate connector Flying Lead Cable Vented and Non Vented A flying lead cable option is available which is intended for wiring to a data collection platform DCP or a data logger A vented flying lead option is for use with a vented sonde only See section 7 for more information Cable Options 599431 01 EXO Cable Coupler Titanium 599040 250 l EXO 250 meter Field Cable 599040 200 EXO 200 meter Fie 599210 33 EXO 33 meter VENTED Flying Lead Cable Extended Field Cables Precaution Be aware
42. C to the sonde however the display units can be set to degrees F and the temperature reading is converted After the units are set data viewed on the Run menu will be displayed in this format Firmware Check and update firmware on connected devices KOR automatically searches for connected sondes sensors and handhelds and loads the table with sensor names serial numbers and current firmware revisions To update firmware see instructions in sections 6 7 and 6 11 Calibration To speed the calibration process users can set default calibration settings for individual sensors and parameters here Click the Apply button and changes take effect immediately Sync with Handheld Upload files from Handheld to PC See section 3 8 pg 73 KOR Software Connections Menu This menu allows the user to connect to other sondes and devices identify which probes are installed in which ports and update Bluetooth settings This submenu allows the user to refresh and initiate connections to hard wired devices search for Bluetooth connections or disconnect To reconnect to a sonde wait for KOR to scan the devices then select a device from the list Click Connect Map This menu allows the user to view connected sondes sensors serial numbers and the ports assigned to the sensors Also if a sonde is connected to the auxiliary port of an EXO2 sonde then both sondes will be visible in this menu However the user can only communica
43. EXO User Manual ADVANCED WATER QUALITY MONITORING PLATFORM Revision THIS IS AN INTERACTIVE DOCUMENT When viewing this document as an Adobe PDF hovering your cursor over certain phrases will bring up the finger point icon Clicking elements of the Table of Contents website URLs or references asking you to see a certain section will take automatically to those locations No scrolling required The information contained in this manual is subject to change without notice Effort has been made to make the information in this manual complete accurate and current The manufacturer shall not be held responsible for errors or omissions in this manual Consult EXOwater com for the most up to date version of this manual Table of Contents 1 Introduction 1 1 1 2 1 3 1 4 1 5 EXO1 Sonde Overview EXO2 Sonde Overview EXO Handheld Overview EXO Cable Overview EXO Sensors Overview and Specifications 1 6 Conductivity Temperature 1 7 Depth and Level 1 8 Dissolved Oxygen 1 9 fDOM 1 10 Ion selective Electrodes ISEs Ammonium Nitrate and Chloride 1 11 pH and ORP 1 12 Total Algae Chlorophyll and Blue green Algae 1 13 Turbidity 2 Operation 2 1 2 2 2 3 2 4 2 5 2 6 2 7 2 8 z _ _ Install Batteries Install Remove Sensor Guard or Calibration Cup Install Remove Sensors Install Saltwater Sacrificial Anode Install Coastal Anode Guard Weight Install Remove Anti fouling Sleeves
44. If a wired connection is not found and to manually establish a connection to a sonde via Bluetooth navigate to the Connections menu in KOR software on the Handheld Select Rescan KOR will rescan and detect Bluetooth enabled sondes Select the sonde from the list and then click the Connect button If no sonde is detected click Refresh to scan again It may take 2 3 scans to discover the sonde pg 41 2 11 Install KOR Software The desktop KOR software is supplied will all EXO sondes on a USB flash drive Installing the software will require Administrative privileges on the local PC It is important to install KOR software prior to using the USB Signal Adapter as the required drivers for the adapter are installed along with KOR software NOTE A lite version of KOR software on the EXO handheld does not require any installation 1 oe Kor pg 42 Install KOR software and drivers Insert USB drive and install software using the startup exe file Select all these items to install EXO KOR software National Instruments supporting software and USB drivers for the EXO USB adapter When complete KOR will reside in the root Program menu not in a subfolder with the following icon e KOR EXO Additionally a folder called National Instruments will be created however this information will not be accessed through the course of normal operation Reboot the computer after installation of the software
45. Minimum requirements Minimum requirements on a computer for KOR software e Windows XP service pack 3 or newer Windows operating platform Windows 7 recommended e Microsoft NET any version from 2 0 through 3 5 Service Pack 1 e 500 MB of hard disk space 1 GB recommended e 2 GB of RAM 4 GB recommended e Screen with resolution of 1280x800 or greater e Available USB 2 0 port e Internet access for software updates e Optional Integral Bluetooth or USB dongle Bluetooth adapter Software updates When they become available updated versions of KOR software will be posted to EXOwater com Users will need to register a free account to access the software download Download and install a Microsoft executable file that helps your computer run applications developed using the NET framework www microsoft com download Search for dotnetfx exe Connect Sonde USB The USB signal output adapter USB SOA 599810 allows users to connect to an EXO sonde over a standard USB connection Although the USB SOA is rugged and water resistant users should protect its connectors with the included cap when not in use The SOA should never be submerged Prior to use users must install KOR software and its drivers on the associated PC The USB SOA will not work without the drivers that accompany KOR See section 2 11 1 Connect SOA to sonde Remove the plug from the 6 pin connector on the sonde Apply a light layer of Krytox grea
46. RS 232 settings e Save only Save but do not deploy and log e Cancel Stop deployment This Stop Deployment button appears when an attached EXO device is actively logging After stopping a deployment the button disappears and the icon in the upper right changes state to indicate the sonde is not actively logging data Advanced sampling and logging Access the following functions in the Deploy Open Tem plate Edit Template menu and click on the Advanced tab Logging Intervals When deploying a sonde data is logged and time stamped at a routine sampling interval A typical sampling interval for unattended logging is 15 minutes If the sonde logs at 00 15 00 the sonde will wake up early to activate the sensors and start processing data Typically this will be 12 15 seconds before the time stamp If an averaging interval were activated then the sonde would wake up 15 seconds early plus the averaging interval to start averaging the data pg 69 Samples Per Wipe Wiping Interval In most deployments the user will choose to use the EXO2 central wiper to wipe the sensors preceding each logging interval We recommend a wiping interval of 1 for 15 minute and 30 minute sampling intervals 1 wipe will occur just prior to a sample being taken Set the wiping interval to 2 and one wipe will occur every other sample If you have a short sampling interval such as 5 minutes and biofouling is not aggressive then you may not need to w
47. Requirements of the listed Directives and Standards and carry the CE mark accordingly Manufacturer YSI Incorporated a Xylem brand 1725 Brannum Lane Yellow Springs OH 45387 USA Equipment name EXO Sondes EXO1 and EXO2 and EXO Handheld Systems Model numbers 599501 xx 599511 xx 599502 xx 599512 xx 599150 Accessories Sensors 599090 xx 599100 xx 599101 xx 599102 xx 599104 xx 599118 xx 599800 599810 599870 xx 599040 xx 599008 xx EXOISEOx Intentional Radiators EXO Sondes EXO1 and EXO2 contain the LMX Bluetooth module EXO Hand held 599150 contains a Wi Fi Bluetooth module Nemko certified body ID CE 2302 Directives e EMC 2004 108 EC e R amp TTE 1999 5 EC e ROHS e WEEE Harmonized Standards e EN61326 1 2006 Electrical equipment for measurement control and laboratory use EMC require ments Part 1 General Requirements e EN61326 2 3 2006 Electrical equipment for measurement control and laboratory use EMC require ments Part 2 3 Particular Requirements Test configuration operational conditions and performance criteria for transducers with integrated or remote signal conditioning e EN61000 3 2 2006 A1 2009 A2 2009 Electromagnetic compatibility EMC Part 3 2 Limits Limits for harmonic current emissions equipment input current lt 16A per phase e EN61000 3 3 2008 Electromagnetic compatibility EMC Part 3 3 Limits Limitation of voltage changes voltage fluctuations and flicker
48. SB SDI 12 RS 232 2 D size batteries Temperature Operating 5 to 50 C Storage i 20 to 80 C Dimensions Diameter 4 70 cm 1 85 in Length 64 77 cm 25 50 in Weight 1 42 kg 3 15 lb w batt pg 5 EXO1 Sonde 59950 xx Removable Bail 599473 6 Pin Cable Connector Upper Battery Compartment Seal O ring kit 599680 Battery Compartment Battery Cover 599052 Lower Battery Compartment Seal Pressure Transducer Opening Red LED Indicator Sonde Status Blue LED Indicator Bluetooth On Off Magnetic Switch for Power and Bluetooth Bulkhead Sensors Port Plug 599475 Calibration Cup 599786 Sensor Guard 599666 599563 Guard Weight 599471 EXO2 Sonde Overview The EXO2 sonde is a multiparameter instrument that collects water quality data The sonde collects the data with up to six user replaceable sensors and an integral pressure transducer Each sensor measures its parameter via a variety of electrochemical optical or physical detection methods Each port accepts any EXO sensor and automatically recognizes the type of sensor Depending on user defined settings the EXO2 will collect data and store it onboard the sonde transfer the data to a data collection platform DCP or relay it to a user s PC or EXO Handheld via cable USB connection or Bluetooth connection In addition to six standard sensor ports the EXO2 also has a bulkhead port for a central wiper
49. SDI 12 pg 48 9 16V DC Ground Voltage The sonde is designed to run with 12 volt batteries with supply voltages between 9 and 16 V DC These systems should be directly powered by a sealed battery or installed as part of a remote solar system Power the SOA through a 1 amp slow blow fuse for protection Remove the fuse until all wiring is completed NOTE External power to the DCP adapter is required even if you are putting batteries in the sonde When used with a solar system always ensure use of 12 volt solar regulator Never connect the sonde directly to solar panel voltages above 16 5 volts will cause the sonde to shut down and excessive voltage will permanently damage the sonde and is not covered under warranty Signal Output Adapter DCP Sonde 9 16VDC IN GND SHLD BARE SPARE ORG COM b WHT COM a YEL GND BLK 232 1 232 RX GND SDI 12 DCP SOA Command Sets To program the DCP with SDI 12 or RS 232 commands please see command sets in KOR software section 4 11 Advanced RS 232 interface commands are available from technical service for system integrators interested in connecting to EXO via RS 232 Connect Sonde Flow Cell There are two versions of the EXO flow cell EXO1 flow cell 599080 and EXO2 flow cell 599201 Flow rate of the flow cell is typically between 100 mL and 1 L per minute Maximum flow rate depends on tubing type size and length Maximum pressure for each flow cell is 25 psi
50. Sonde States and LED Descriptions Awake Sonde Activate Bluetooth Attach Sonde to Handheld 2 9 Field Cable 2 10 Bluetooth Wireless Communication Install KOR Software Connect Sonde 2 12 USB 2 13 Bluetooth Link to PC 2 14 Data Collection Platform 2 15 Flow Cell 2 16 Connect Field Cables with Cable Coupler 3 Handheld 3 1 3 2 3 3 3 8 3 9 Install Batteries Rechargeable Li Ion Battery Pack Overview Power On Off Handheld Attach Handheld to Sonde 3 4 Field Cable 3 5 Bluetooth Wireless Communication 3 6 Spot Sampling View Live Data 3 7 Collecting Data with Handheld Managing Sites Upload Data Handheld to PC 3 10 Handheld Options A KOR Software 4 1 Introduction to Navigation 4 2 Run Menu 4 3 Calibrate Menu 4 4 Deploy Menu 4 5 Sites Menu 4 6 Data Menu 4 7 Options Menu 4 8 Connections Menu 4 9 Help Menu 4 10 Data Files and File Locations 4 11 SDI 12 and RS 232 Commands 4 12 Update Firmware and Software Sonde and Sensors Handheld Calibration 5 1 Introduction to Basic Procedure 5 2 Calibrating Conductivity Temperature 5 3 Calibrating Dissolved Oxygen 5 4 Calibrating Depth 5 5 Calibrating pH 5 6 Calibrating ORP 5 7 Calibrating Turbidity 5 8 Calibrating Total Algae Chlorophyll and Blue green Algae 5 9 Calibrating DOM 5 10 Calibrating ISEs Ammonium Nitrate and Chloride 5 11 Calibration Standards 5 12 Calibration Record Sheet Maintenance 6 1 Sonde Storage
51. Standard for Chloride Sensor A Read and follow all the safety instructions and MSDS documentation supplied with the chemical before proceeding Remember that only trained personnel should handle hazardous chemicals Preparation Use the following procedure to prepare 10 and 1000 mg L chloride reagents for the EXO Chloride sensor Nitrate and Ammonium standards can be purchased from YSI or other laboratory supply companies 1000 mg L Standard 1 2 3 4 5 Purchase solid sodium chloride from a supplier Accurately weigh 1 655 grams of anhydrous sodium chloride and transfer into a 1000 mL volumetric flask Add 0 5 grams of anhydrous magnesium sulfate to the flask Add 500 mL of water to the flask swirl to dissolve all of the reagents Dilute to the volumetric mark with water Mix well by repeated inversion and then transfer the 1000 mg L standard to a storage bottle Rinse the flask extensively with water prior to its use in the preparation of the 10 mg L standard Alternatively simply add 0 5 grams of magnesium sulfate to a liter of a 1000 mg L chloride standard from a certified supplier 10 mg L Standard 1 2 3 Accurately measure 10 mL of the above 1000 mg L standard solution into a 1000 mL volumetric flask Add 0 5 grams of anhydrous magnesium sulfate to the flask Add 500 mL of water swirl to dissolve the solid reagents and then dilute to the volumetric mark with water Mix well by repeated inversion and then transfer
52. Toaster Oven Pre heat oven to approximately 65 70 C A DO NOT exceed 80 C or you will damage components of the sonde and sensors Insert the equipment by gripping the sonde by the bail and not by the sonde s sleeve If possible the sonde should be in the upright position when placed in the oven Laying the sonde on its side resting on a grate or rack could cause inconsistencies in the shrinking process Safely remove it with gloves once the sleeves visibly cling to the instrument NOTE With either method small air bubbles may appear in the sleeve This is expected and will not affect the performance of the sleeve 5 Removing the sleeves after use To remove the sleeves find the perforation in the plastic Pull down on this from the top If unable to remove the sleeve with this approach simply cut the plastic Do so cautiously so as to not damage the instrument A DO NOT use a sharp instrument in an attempt to cut the sleeve off This could cut through the sleeve and into your sonde or probe causing damage to your equipment pg 37 27 Sonde States and wap LED Descriptions States An EXO sonde is always in one of three operational states Off Awake and Asleep These states determine the sondes current power usage and logging potential When Off the sonde is not powered and cannot collect data no batteries installed no topside power Users can apply power to the sonde internally using batteries or externally with an E
53. XO field cable attached from the topside port to an EXO Handheld DCP or other approved power source Once power is applied to a sonde it is either Awake or Asleep States Off Not powered no data collection Asleep Low power Waiting for command Awake Full power Ready to collect LED Indicators Blue LED Bluetooth None Off not active On Solid On not linked 2 Hz Blink On successfully linked Red LED Sonde State None Sonde is Off or Asleep with logging disabled 0 1 Hz Blink Sonde is Asleep with logging enabled 1 Hz Blink Sonde is Awake On Sonde is Awake with faults pg 38 When in an Asleep state the sonde remains in a very low power setting and waits for a user command or its next scheduled logging interval An Awake sonde is fully powered and ready to collect data Once awakened a sonde remains Awake for five minutes after its last communication via Bluetooth or 30 seconds after its last communication via the topside port The sonde also automatically awakens 15 seconds before its next scheduled logging interval LED Indicators Each sonde has two LED indicators that show the sondes status The blue LED indicates the Bluetooth s wireless connection status The red LED indicates the sonde s current state The Bluetooth light blue is activated by a magnet swipe at the magnetic activation area When the blue LED is off the Bluetooth is disabled When the light is on continuously
54. and EXO Handheld Systems Model numbers 599501 xx 599511 xx 599502 xx 599512 xx 599150 Intentional Radiators EXO Sondes EXO1 and EXO2 contain the LMX Bluetooth module Broadcasting and certification number KCC CRM XYL EXOSondel for EXO1 and KCC CRM XYL EXOSonde2 for EXO2 EXO Handheld 599150 contains a Wi Fi Bluetooth module Broadcasting and certification number KCC CRM XYL EXOHANDHELD for EXO Handheld Type Identification LARNS8 102Y2402 2480TR0 000003F1D79 EXO1 LARNS8 1I02Y2402 2480TRO 00001F1D79 EXO2 LARN8 IO2Y2402 2480TRO 00003F1DG1D79 EXO Handheld Regulation Radio Waves Act of the Republic of Korea As J1 S28 YSA IAM o IIE SSS AS ANT SEDIA SHOWA FE ASHE Ol SS SHA Stet HH A AYA ASIA ASSE AS SAS e Bue Class A device Broadcasting and communication equipment for office work Seller and user shall be noticed that this equipment is suitable for electromagnetic equipment for office work Class A and it can be used outside the home KCC notice 2012 12 Radio device using 2400 2483 5 MHz and 5725 5825 MHz de FAE ATES Jao QeSe AAD Hae AbAE Ss AS Service related to human safety is not allowed because this device may have the possibility of the radio interference VELL Lisa M Abel Director Quality December 13 2012 pg 149 The undersigned hereby declares that the products listed below conform with all applicable requirements of the Radio Regulations of China for i
55. ant change for approximately 40 seconds click Apply to accept this calibration point If the data do not stabilize after 40 seconds gently rotate the sonde or remove reinstall the cal cup to make sure there are no air bubbles in the conductivity cell Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde and sensor s in tap or purified water and dry pg 82 Calibration Dissolved Oxygen First review the basic calibration description in section 5 1 ODO sat and ODO local 1 point Place the sonde with sensor into either saturated air or saturated water a Saturated air Ensure there are no water droplets on the DO sensor or the thermistor Place into a calibration cup containing about 1 8 inch of water that is vented by loosening the threads Do not seal the cup to the sonde Wait 10 15 minutes before proceeding to allow the temperature and oxygen pressure to equilibrate Keep out of direct sunlight b Saturated water Place into a container of water which has been continuously sparged with an aquarium pump and air stone for one hour Wait approximately 5 minutes before proceeding to allow the temperature and oxygen pressure to equilibrate In the Calibrate menu select ODO then select ODO sat or ODO local Calibrating in ODO sat automatically calibrates ODO mg L and ODO lo
56. anty 3 Level sensor storage Users can store these sensors either dry or submerged in clean water However regardless of storage method or length ensure the vent tube remains dry Always attach the port plug to the cable connector or leave the cable installed with a cap over the desiccant s vent 4 Level desiccant maintenance Active desiccant is blue saturated desiccant is pink When the desiccant closest to the sonde begins to turn pink you should replace YSI 6108 or regenerate YSI 6109 the desiccant cartridge To regenerate desiccant remove it from the cartridge and heat it for one hour at 200 C about 400 F then cool it an airtight container before refilling Also heat the felt filters at 100 C about 200 F for 30 minutes The desiccant will turn blue following a successful recharge og 117 6 13 Standard Optical Sensor Maintenance and Storage Standard optical sensors include Turbidity Total Algae and DOM sensors these optical sensors are very low maintenance This section identifies storage as long term or short term Long term denotes storage during times of long inactivity over winter end of monitoring season etc Short term denotes storage during times the sonde will be used at a regular interval daily weekly biweekly etc Maintain connectors as instructed in section 6 24 1 Clean sensing window Turbidity Total Algae and DOM require minimal maintenance Users sho
57. ard solution for a few minutes after exposure In addition be particularly careful that readings are stable during subsequent calibrations Sensor Drift The ion selective electrodes have the greatest tendency to exhibit calibration drift over time This drift should not be a major issue for sampling studies where the instrument can be frequently calibrated However if the sensor is used in longer term deployments drift is almost certain to occur The extent of the drift will vary depending on the age of the probe the flow rate at the site and the quality of the water For all monitoring studies using ion selective electrodes the user should acquire a few grab samples during the deployment for analysis in the laboratory or with another sensor that has been recently calibrated Sensor Accuracy Specifications The typical accuracy specification for the sensors 10 of reading or 2mg L which ever is greater for ammonium and nitrate and 15 of reading or 5mg L which ever is greater for chloride refer to sampling applications where only minimal time has elapsed between calibration and field use To maintain accuracy specifications for EXO sensor we recommend that users calibrate sensors in the lab in standards with temperatures as close to the ambient temperature of the field water as possible All ion selective electrodes are subject to the interaction of species with the sensor membrane which are similar in nature to the analyte These interf
58. are stable Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde and sensors in tap or purified water and dry pg 86 Calibration ORP Review the basic calibration description in section 5 1 Pour the correct amount of standard with a known oxidation reduction potential value we recommend Zobell solution in a clean and dry or pre rinsed calibration cup Carefully immerse the probe end of the sonde into the solution In the Calibrate menu select pH ORP then select ORP mV Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point A Do not leave sensors in Zobell solution for a long time A chemical reaction occurs with the copper on the sonde sonde bulkhead central wiper assembly copper tape While the reaction does not impact calibration it will degrade the sonde materials over time Discard the used standard Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde Effect of temperature on ORP The oxidation reduction potential value shows an i
59. arometric pressure is overcome by proper sensor calibration to a standard pressure However if the user measures dissolved oxygen in something besides per cent saturation then the EXO DO sensor can store a local barometric reading put into the KOR software DO local or the EXO handheld can take a live barometric reading with its internal barometer ODO EU ODO Sat Raw DO reading corrected with temperature and local barometric pressure at the time of calibration Local pressure 760 mmHg x 100 Sat ODO Local Raw DO reading corrected with temperature and Sat output fixed to 100 regardless of barometric pressure entry The entered local barometric pressure is used by KOR software for mg L calculations ODO EU ODO Sat reading corrected with live barometric reading available only on EXO Handheld Fixes the Sat output to 100 and conforms to British and EU standards 6g 20 fDOM Sensor Overview The EXO fDOM Fluorescent Dissolved Organic Matter sensor is a fluorescence sensor which detects the fluorescent component of DOM Dissolved Organic Matter when exposed to near ultraviolet UV light Colored Dissolved Organic Matter Users might wish to quantify colored dissolved organic matter CDOM in order to determine the amount of light which is absorbed by stained water and thus is not available for the photosynthesis process carried out by subsurface aquatic plants and algae In most cases f DOM can be used as
60. artment Opening Battery Compartment On Off Magnetic Switch for Power and Bluetooth Red LED Indicator Sonde Status Blue LED Indicator Bluetooth Bulkhead Sensors Port Plug 599475 Sensor Guard 599667 599564 Calibration Cup 599316 Central Wiper 599090 01 Guard Weight 599472 EXO Handheld Overview The EXO Handheld HH is a rugged microcomputer based instrument that allows the user to display sonde readings configure sondes store and retrieve data and transfer data from sondes to a computer Equipped with GPS barometer and custom operating system the Handheld communicates via Bluetooth wireless technology field cable or USB connector NOTE The USB connection is only used when connecting the Handheld to a PC The unit utilizes an adjustable backlit screen for easy day or night viewing Pre installed KOR software facilitates all user interaction and provides powerful control over data collection Top View USB Port Speaker RE a GPS Antenna internal Tripod Mount jose Barometer Handstrap Mount Back View Battery Cover 1 b Handstrap Mount Specifications Barometer Temperature Operating Storage Dimensions Width length Weight w batt Polycarbonate ABS housing rated to IP O7 in factory tests polycarbonate lens Bluetooth Field Cable 4 C size alkaline batteries or optional Li lon Pack 5 to 50
61. at houses the processing electronics memory and wet mate connector This allows users to reduce the costs associated with these sensors by only replacing the relatively inexpensive module periodically and not the more costly base The connection of the module to the sensor base is designed for one connection only and the procedure must be conducted in an indoor and dry environment Once installed the module cannot be removed until you are prepared to replace it with a new module See section 6 21 for detailed instructions The typical life expectancy of an ISE sensor is three to six months depending on use AN Precautions e ISEs are intended for sampling purposes and must be calibrated frequently due to sensor drift e ISEs can be used in long term deployments for qualitative trends Use with an EXO wiper will deform the brush over time and may require more frequent brush replacement The brush deformation may intensify with the fouling present in the monitored environment e ISE sensors only come in guarded configurations Customers should not remove the plastic guard that protects the ISE membrane e For long term deployments sensor data should be compared to that of grab samples throughout the monitoring period to note drift For a full list of precautions see the end of section 5 10 aloe pH and ORP Sensor Overview Users can choose between a pH sensor or a combination pH ORP sensor to measure these parameters pH
62. at left which help reduce vibration against the battery cover EXO1 and EXO2 Insert the batteries with positive terminals facing up and negative terminals facing EXO top down toward the probes 3 Replace battery cover NOTE Before replacing the battery cover inspect and service the o rings Ensure that the o rings are not nicked or torn and that they have no contaminants or particles on them or the sealing surfaces inside the battery cover Then apply a thin coat of Krytox lubricant to each o ring and sealing surface Replace the battery cover or cap and tighten until snug Do not overtighten pel 31 27 Install Remove Guard or Calibration Cup Sonde guards protect EXO sensors from impact throughout deployment Users must install the guard prior to data collection The calibration cup cal cup is used for storage and calibration We recommend using two guards one for field deployments and a second used exclusively for calibrations Using a second guard will minimize calibration solution contamination especially for turbidity EXO calibration cups install over an installed sonde guard This configuration reduces the amount of standards required for calibration and protects the sensors during calibration 1_Install remove sonde guard Install guard by threading it onto the sonde bulkhead threads Rotate the guard clockwise on the bulkhead to install taking care not to pinch your fingers Rotate it counterclock
63. ata Filtering All EXO sensors share some common embedded software including the filtering of real time data Sensors acquire environmental data at a constant rate and use this stream of data as the input to the filtering algorithm that produces results seen by the user EXO sondes collect data from the EXO sensors and are able to output data at rates up to 4 Hz The EXO sensor data filtering process consists of four components none of which is user selectable Basic Rolling Filter The filter is fundamentally a rolling or window average of past acquired inputs to the filter such that as a new data value is added to the summation the oldest data value is removed and the total summation is divided by the total number of data values It is a simple average just rolling or moving in time Starting with the February 2014 software release different rolling time windows for the filter are now supported Data Filtering Modes Data filtering options are included in the handheld and desktop version of KOR These settings can be modified within the Sonde Options menu Options gt Sonde as well as within the deployment template settings NOTE Making any changes to data filtering options will stop a deployment As a sonde takes measurements it compares new readings to those taken in the previous 2 30 seconds depending on the selected option If the new reading is not significantly different than past measurements then it merely factors into the rolling
64. ation Defaults under the same Advanced Menu Calibrating multiple sensors To calibrate multiple sensors of the same type concurrently install all the sensors in the sonde In the Calibration menu click on the sensor and new options are available in the second menu for calibrating ALL like sensors Follow the calibration instructions in KOR to calibrate all the sensors Calibrations occur one after the other not simultaneously Sensors are turned on one at a time so the user can validate each sensor s reading and to avoid the possibility of interference from the other sensors Once all like sensors have been calibrated they can be removed from the sonde and installed in other EXO sondes The sensor will retain its calibration Other ODO_sat probes are present Would you like to calibrate them too Note When calibrating multiple sensors with the Handheld clicking the Restore Default Calibrations button will restore ALL like sensors to original settings Note Menu functionality is the same although visual display differences are found between KORS Desktop version top and middle and Handheld version bottom this page and previous page pg 67 KOR Software Deploy Menu The Deploy menu is used mainly to configure an EXO sonde to collect unattended data and to manage deployment templates This menu is dynamic based on the mode of the attached device Two or three submenu options are available Read Current Set
65. ay also store the sensor by submerging the cap end in water J If pH sensor is also installed do not submerge it in distilled water pg 123 6 18 pH and pH ORP Sensors Storage and Rehydration pH and pH ORP sensors have two specific storage requirements they should not be stored in distilled or deionized water and their reference electrode junction should never dry out This section will identify storage as long term or short term Long term denotes storage during times of long inactivity over wintering end of monitoring season etc Short term denotes storage during times the sonde will be used at a regular interval daily weekly biweekly etc 1 Short term storage When in regular field use the sensor should remain installed on the sonde in an environment of water saturated air Place approximately 0 5 in 1 cm of any water deionized distilled tap or environmental in the bottom of the calibration cup Insert the sonde and sensor into the cup and screw it on tightly to prevent evaporation More information in Short Term Sonde Storage section 6 1 2 Long term storage Remove the sensor from the sonde and insert its sensing end into the bottle that the sensor was shipped in Install the bottle s o ring and cap then tighten This bottle contains a 2 molar solution of pH 4 buffer If this solution is unavailable users may store the sensor in tap water A Do not store the pH or pH ORP sensor
66. ble for the EXO turbidity sensor 12 4 FNU EXO 12 7 NTU YSI 6 Series 1 gallon 124 FNU EXO 126 NTU YSI ell 1 gallon O10 FNU ee 1000 NTU 607400 YSI 6 Series 1 gallon pg 89 Er Calibration Chl BGA Total Algae Review the basic calibration description in section 5 1 Before calibrating be certain that the sensing window is clean cleaning instructions section 6 13 Chlorophyll This procedure calibrates Chlorophyll RFU or Chlorophyll ug L If the user has both units selected then this procedure must be performed twice once for each unit to completely calibrate the parameter For 2 point calibrations one standard must be clear water 0 ug L and this standard must be calibrated first The other standard should be in the range of a known chlorophyll content of the water to be monitored Two general types of standards can be used a phytoplankton suspensions of known chlorophyll content determined by employing the extractive analysis procedure described in Standard Methods for the Examination of Water and Wastewater or by analyzing the suspension in situ using a laboratory fluorometer and b dye solutions whose fluorescence can be correlated to that of chlorophyll For option b we recommend using a 625 ug L Rhodamine WT dye solution for detailed instructions see section 5 11 and the solution is used in the calibration steps below ug L 1 or 2 point This procedure will zero your fluor
67. bmersion during storage may produce sensor drift Users should aim for a storage environment of water saturated air 100 humidity for the sensors Place approximately 0 5 in 1 cm of water deionized distilled tap or environmental in the bottom of the calibration cup Then place the sonde with all of its sensors into the cup and close it tightly to prevent evaporation Users can also use a moist sponge to create a humid environment Ensure that unused sensor ports are properly protected with port plugs The sonde itself should be stored in dry air To protect the cable connector either leave the cable installed on the connector or install the port plug This is especially important for sondes with level users should always keep the cable connector of vented sondes dry See section 7 5 2 Long term storage Store all removed sensors according to the specific instructions in their sensor storage section Plug all open ports and store the sonde according the above instructions for short term sonde storage A Always remove batteries from sondes during long periods of inactivity to prevent potentially harmful battery leaks pg 103 Sonde Maintenance Like all precision equipment EXO sondes work most reliably when users maintain them properly A proper inspection and cleaning can prevent several issues including leaks When performing general maintenance on the sonde also check this manuals depth and connector secti
68. bration description in section 5 1 This procedure calibrates conductivity non linear function nLF conductivity specific conductance salinity and total dissolved solids Place the correct amount of conductivity standard into a clean and dry or pre rinsed calibration cup A variety of standards are available based on the salinity of your environment Select the appropriate calibration standard for your deployment environment we recommend using standards greater than 1 mS cm 1000 uS cm for greatest stability Carefully immerse the probe end of the sonde into the solution making sure the standard is above the vent holes on the conductivity sensor Gently rotate and or move the sonde up and down to remove any bubbles from the conductivity cell Allow at least one minute for temperature equilibration before proceeding In the Calibrate menu select Conductivity and then a second menu will offer the options of calibrating conductivity nLF conductivity specific conductance or salinity Calibrating any one option automatically calibrates the other parameters After selecting the option of choice specific conductance is normally recommended enter the value of the standard used during calibration Be certain that the units are correct and match the units displayed in the second window at the top of the menu Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no signific
69. cal and vice versa Enter the current barometric pressure in mm of Hg Inches of Hg x 25 4 mm Hg NOTE Laboratory barometer readings are usually true uncorrected values of air pressure and can be used as is for oxygen calibration Weather service readings are usually not true i e they are corrected to sea level and therefore cannot be used until they are uncorrected An approximate formula for this uncorrection where the BP readings MUST be in mm Hg is True BP Corrected BP 2 5 Local Altitude in ft above sea level 100 Click 1 Point for the Calibration Points Enter the standard value air saturated Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu mg L l point Place the sonde with sensor in a container which contains a known concentration of dissolved oxygen in mg L and that is within 10 of air saturation as determined by one of the following methods Winkler titration Aerating the solution and assuming that it is saturated Measurement with another instrument uN Carrying out DO mg L calibrations at values outside the range of 10
70. cedure for ammonium and nitrate except that the calibration standard solution values are in mg L Cl instead of NH N or NO N YSI recommends that the user employ standards for chloride that are 10 times greater than for ammonium and nitrate and that span the expected deployment conditions Typical calibration ranges are 10mg L Cl and 1000mg L CI or 1000mg L Cl and 18000mg L Cl pg 97 ISE Precautions e Ion selective electrodes may not stabilize as rapidly as pH sensors Be sure to allow plenty of time for the readings to come to their final values during all calibration routines e Ion selective electrodes generally drift more than pH sensors To check for this drift read the sensor s value in a calibration standard solution at the end of each deployment e Ammonium and nitrate standards are good growth media for a variety of organisms This growth can significantly reduce the nitrogen content of your standards an effect that is particularly important for the 1 mg L solution It is best to use new standards for each deployment but if you decide to save your solutions for reuse we recommend refrigerated storage to minimize the growth of these organisms e Remember that the ammonium nitrate and chloride sensors will take longer to stabilize after exposure to high conductivity solutions such as a pH calibration To accelerate the recovery process soak the sensor in 100 mg L ammonium or nitrate standard solution or 1000mg L Cl stand
71. cess the Handheld should remain stationary and have a clear view of the sky The first time a user powers on the Handheld the GPS fix can take up to a maximum of 20 minutes to obtain To manually enable GPS go to the Options menu Select age e English gt i Ken Handheld Enable GPS On Click Apply When GPS is Ci fixed a signal strength icon with green bars will appear If ll the bars are black then no GPS signal is found Sandve E Normal S Hibernate min The GPS consumes battery power and can be turned off to Time Display Mode conserve battery life 2 Display GPS units To display GPS units on screen go to the Options Units menu and select GPS Lati and GPS Long When units are selected GPS data is displayed on screen only if the GPS is Cable Pwr Y 0 turned on 5 om na NOTE If the Handheld is stored for more than 10 days without batteries installed then the user will need to re enter the date and time Additionally it will take longer for the unit to obtain a new GPS fix s 1 Turn on off GPS 09 17 Capture Data Live Graph Additional Handheld Options on the next page pg 61 EST Handheld Options Continued Additional options in the Handheld menu and a brief description of their function After making a change to a selection the field will be highlighted in green Be sure to hit the top right Apply button to confirm your changes Language Settings gt Handheld gt
72. charge any product that we determine to be covered by this warranty To exercise this warranty write or call your local representative or contact Technical Support Send the product and proof of purchase transportation prepaid to the Authorized Service Center selected by the manufacturer Repair or replacement will be made and the product returned transportation prepaid Repaired or replaced products are warranted for the balance of the original warranty period or at least 90 days from date of repair or replacement Limitation of Warranty This Warranty does not apply to any EXO product damage or failure caused by i failure to install operate or use the product in accordance with the written instructions ii abuse or misuse of the product iii failure to maintain the product in accordance with the written instructions or standard industry procedure iv any improper repairs to the product v use by you of defective or improper components or parts in servicing or repairing the product or vi modification of the product in any way not expressly authorized by the manufacturer THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY IN NO EVENT SHALL YSI BE
73. ck Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde pg 88 3 point Select the 3 point calibration option for maximum accuracy over a wider range As for the 2 point procedure the first standard must be 0 FNU Because of the linearity characteristics of the sensors we recommend that the other two standards have turbidity values of 124 and 1010 FNU It is important to use a consistent type of standard for all calibration points The procedure for this calibration is the same as for a 2 point calibration but the software will prompt you to proceed to an additional solution to complete the 3 point procedure Calibration Limits Due to the non linear response of the turbidity sensor calibration ranges may be limited A 1 2 or 3 point calibration may be completed using the following limits 1 point 2 point 3 point 1 FNU 5199FNU 200 4200 FNU or NTU or NTU or NTU Calibration standards The following standards are availa
74. crificial anode is commonly used in marine applications to minimize corrosion damage to all metallic parts of ships Elemental zinc is used as the sacrificial anode because it is more easily oxidized than most other metals A replaceable block of zinc is installed in electrical contact with other metals that are susceptible to saltwater corrosion and the zinc is preferentially oxidized or corroded thus preventing damage to other metal components The metal on the EXO s bulkhead probe housings and optional antifouling guard benefit from the use of a sacrificial anode NOTE Early 2014 sonde models include a titanium bulkhead and connectors This new feature eliminates the need to use an anode however all older sondes with a bronze alloy do require an anode to prevent damage 1 Grease threads Apply a light coating of lubricant to the connector threads to prevent binding Apply a light coating of lubricant to the anode base s o ring and threads 2 Install the anode base Insert the anode base in an available sensor port and screw down the retaining ring until snug 3 Install the anode Install the sacrificial anode in the anode base twisting until anode is snug When the first anode deteriorates replace with the second anode in the kit When both anodes have been consumed purchase a new anode kit pg 34 2 5 Install Coastal Anode Guard Weight The EXO Coastal Anode Guard Weight is the alternative zinc solution
75. cted device by serial number and name The sonde icons show if an EXO sonde is connected check mark or not connected X The folder icons show if the sonde is currently in logging mode check mark or not logging X pg 63 KOR Software Run Menu The Run menu displays real time water quality data in numeric or graph formats and has three main submenus oe Z0 BO Dashboard This real time display shows data from connected devices seroma A The data appears in a default order of parameters in the list Inthe RUN menu data is automatically buffered up to 1000 points in desktop KOR He Capture Data Use the Capture Data button to save a snapshot of the data buffer to the computer The captured data is automatically saved in the location and in a format specified in the Settings submenu see next page When capturing data on the Handheld a new menu will appear with various options for sampling data 5 31 BGA PC RFU 4 78 FDOM RFU 41 47 k HOFFA ee Wipe Sensors In the Dashboard menu it is possible to 22 682 57 6 0 21 manually activate the central wiper if the EXO2 sonde Teme EO 2a ee 2 is equipped with one This feature can be helpful when Baro mmHg B BGAPC ugh G transferring the sensors into liquid the wiper can help 78 08 shea Ethene remove any bubbles that may be trapped at the sensor faces o 4 Turb TSS mgA 4 Sonde Batt V 0 PES ae NOTE Menu functionality is the same although vi
76. ctors Connectors on vented level cables have 5 pins and a vent pin Each cable also incorporates a strain relief mechanism to alleviate stress on the connector Because Bluetooth wireless will not pass through water users must use the cable to connect to the sonde when it is submerged and taking real time field readings that are being viewed by the user or logged by a data collection platform 1 Attach cable to sonde Apply a light layer of Krytox grease to the male pins and rubber surface of the connector on the cable and the female connector on the sonde For the EXO1 sonde first attach the cable s strain relief to the sonde s bail with a carabiner Then press in the cable s male 6 pin connector and screw down the retaining collar For the EXO2 sonde the strain relief can be connected after the cable and collar The cable s strain relief is designed to capture the sonde in case of failure of the connector 2 Attach cable to handheld Apply a light layer of Krytox grease to the male pins on the handheld and the female connector on the cable Press on the female 6 pin connector then screw down the retaining collar Connect the strain relief to the Handheld s strap 3 Discover sonde in KOR Upon startup of the Handheld KOR software searches for a hard wired connection to the sonde If KOR discovers the sonde it will request to connect to it For the XG Platform If the Handheld 1S already powered up go to the
77. d go black To put the HH to sleep press and hold the power button for less than three seconds and release it The screen will then go black Attach Handheld to Sonde Field Cable All EXO cables have 6 pin and wet mateable connectors Connectors on vented level cables have 5 pins and a vent pin Each cable also incorporates a strain relief mechanism to alleviate stress on the connector throughout deployment Although the cables are wet mateable users should dry the connectors prior to installation when possible Always protect connectors by leaving the cable or connector caps installed even when the connector is not in use Always attach the cable s strain relief mechanism to the bail when the sonde is deployed For the EX Platform Connecting to Wired Sonde w _ K 0 1 0 561 1 Attach cable to sonde Apply a light layer of Krytox grease to the male pins and rubber surface of the connector on the cable and the female connector on the sonde For the EXO1 sonde first attach the cable s strain relief to the sonde s bail with a carabiner Then press in the cable s male 6 pin connector and screw down the retaining collar For the EXO2 sonde the strain relief can be connected after the cable and collar The cable s strain relief is designed to capture the sonde in case of failure of the connector 2 Attach cable to handheld Apply a light layer of Krytox grease to the male pins on the cable an
78. d the female connector on the handheld Press on the female 6 pin connector then screw down the retaining collar Connect the strain relief to the Handheld s strap Discover sonde in KOR Upon startup of the Handheld KOR software searches for a hard wired connection to the sonde If KOR discovers the sonde it will request to connect to it pg 55 Attach Handheld to Sonde Bluetooth Wireless Users can wirelessly connect their EXO sondes above water to the EXO Handheld using Bluetooth wireless communication With Bluetooth users can reduce the amount of cables needed to operate their sonde The wireless connection has a typical range of 10 meters but this range will fluctuate depending on the operating environment Users cannot wirelessly connect through water In order to connect via wireless both devices must be powered on 1 Activate sonde s Bluetooth Users activate Bluetooth by holding a magnet at the magnetic activation area In addition to magnetic activation users can also activate Bluetooth by cycling power to the sonde remove reinstall batteries XS gt NOTE Starting 2014 a new magnetic tool is available Item 599469 EXO Sensor Tool Kit magnet inside tool 2 Discover sonde in KOR Every time the Handheld powers on it automatically searches for a sonde via the hard wired cable connection on ll and will connect to the sonde if the Auto Discover option is exa enabled in the Connec
79. d working order replace the o rings before use Insert new sensor module Align the prongs on the base of the module with the slots in the sensor body The sensor module is keyed to insert in only one orientation Once the module is aligned press it firmly into position until it clicks Wipe away any excess grease from the assembled components Apply new sticker Wrap the junction of the sensor module and the body with the new sticker included in the sensor module kit This sticker helps keep the sensor module junction clean and retains the rubber plug throughout deployment On the sticker mark the date the replacement module was installed as a reminder Re calibrate the sensor Using KOR software calibrate the sensor following each sensor module replacement After calibration the sensor is ready for field use pg 129 per 6 22 N hi eanes and Storage Follow these instructions to replace the wiper brush assembly or brush guard component on the central wiper module on the EXO2 sonde We recommend changing the wiper between deployments to avoid sediment carryover which can compromise calibration and data collection For long and short term storage the wiper requires minimal precautions Users can either remove the wiper or leave it installed in the sonde If left installed on the sonde follow guidelines for sonde storage If users remove it from the sonde the wiper may be stored in dry air in its shipping cap to
80. describes the acid and base characteristics of water A pH of 7 0 is neutral values below 7 are acidic values above 7 are alkaline ORP designates the oxidizing reducing potential of a water sample and is useful for water which contains a high concentration of redox active species such as the salts of many metals and strong oxidizing chlorine and reducing sulfite ion agents However ORP is a non specific measurement the measured potential is reflective of a combination of the effects of all the dissolved species in the medium Users should be careful not to overinterpret ORP data unless specific information about the site is known continued Specifications pH units Temperature Operating 5 to 50 C Storage i O to 60 C 0 1 pH units within 10 C of calibration temperature 0 2 pH units for entire temp Glass combination electrode millivolts Temperature Operating 5 to 50 C Storage O to 60 C 20 mV in Redox standard solution 599701 599702 599705 599706 59979501 59979502 59979701 599797 02 modules Platinum button pg 25 Replaceable Sensor Module The EXO pH and pH ORP sensors have a unique design that incorporates a user replaceable sensor tip module and a reusable sensor base that houses the processing electronics memory and wet mate connector This allows users to reduce the costs associated with pH and pH ORP sensors by only replacing the relatively inexp
81. e hydration caps or buffer bottles on probes Wipe dry with a clean lint free cloth 2 Clean port and install sensor Visually inspect the port for contamination If the port is dirty or wet clean it with a clean lint free cloth or compressed air Apply a light coat of Krytox grease to the rubber mating surfaces of the connector not the o ring and a small dab of Krytox grease on the threads of the locking nut Insert the sensor into the port by properly aligning the connectors pins and sleeves male and female contacts then press them firmly together 3 Tighten locking nut Taking care not to cross thread the grooves finger tighten the locking nut clockwise When the nut and o ring are seated against the bulkhead tighten the nut with probe tool 1 4 turn until snug Once sensors or plugs are installed reinstall the sensor guard to protect sensors from impact damage Take care not to twist the probe body when tightening and loosening the locking nut Excessive twisting of the probe can damage the connector and is not covered under warranty pe l 33 Install Saltwater Sacrificial Anode One of two EXO saltwater anode kits are required for sondes continuously deployed in corrosive media particularly saltwater or brackish estuarine water This section describes the anode that installs in a sensor port 599595 The next section describes the anode that attaches to a metal sensor guard 599520 or 599521 A sa
82. e sonde via the topside RS 485 interface The SOA will automatically recognize when a sonde is connected and retrieve the SDI 12 address and ID from the sonde The SDI 12 data parameter list is set by the user in the Deploy menu Go to Deploy Open Template Edit Template menu and click on the SDI 12 tab Sensor parameter codes Add Choose the SDI 12 parameters you want from the Available column and i i double click or select and move them with psi Turbidity the right arrow to the Selected column ee i Turbidity Remove Remove parameters from Selected column by double clicking or selecting and clicking the left arrow Order Arrange the parameter output by using the up and down arrows DD MM YY MM DD YY KOR Software RS 232 The EXO DCP Signal Output Adapter SOA supports limited RS 232 commands The SOA supports both SDI 12 and RS 232 communications The order of the RS 232 parameter output is controlled by the SDI 12 tab on the deployment menu See section 2 14 for instructions on mounting and wiring the DCP SOA Commands Returns sonde data as specified by SDI12 parameter settings set by user in Deploy menu of KOR software Lists the parameter order using ID numbers Parameter codes on previous page Sets or gets current time in HH MM SS Starts a wiper event responds with estimate of time in seconds to complete i wiping sequence Verdate i Returns sonde firmware version compila
83. e standard in the calibration cup Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing verify that no air bubbles reside on the sensing face of the sensor If there are bubbles gently shake or move the sensor to dislodge When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde Discard the used standard pg 95 Calibration ISEs Ammonium Nitrate amp Chloride This procedure calibrates the EXO ammonium chloride or nitrate sensor The sensors can be calibrated to one two or three points The 3 point calibration method assures maximum accuracy when the temperature of the media to be monitored cannot be anticipated we strongly recommend a 3 point calibration for best performance of ISE sensors Review the basic calibration description in section 5 1 The temperature response of ion selective electrodes is not as predictable as that of pH sensors Therefore be sure to carry out a 3 point calibration the first time you use the sensor This will provide a default setting for the effect of temperature on your sensor After this initial calibration you can use the less time c
84. e suggest e Installing the sonde in a PVC pipe that has adequate openings for flow e Aiming the sensors north in northern hemisphere or south in southern hemisphere e Using a sun shield if the sonde is in the open pg 138 7 3 Cables Vented cables for EXO have a maximum length of 33 meters so when connecting a sonde to a data logger users should use a junction box to reach further distances In the junction box the EXO cable can connect to the desiccant as well as another cable running to the data logger or DCP device Installation e Avoid bending vented cables sharply to prevent the inner tube from kinking Min bend radius 20 3 cm 8 in e EXO vented cables have a reduced length to prevent tube damage from their own weight e EXO vented cables do not have wet mate connectors any water or humidity entering the vent tube will cause damage to the pressure sensor that is not covered by the warranty e EXO vented cables are not equipped with the barbed fitting for small desiccant cartridges Dessicants A All EXO sondes with vented level require the use of a desiccant Any damage to the sensor due to the lack of desiccant use is not covered under warranty Two desiccant systems are available a cartridge kit YSI 6108 and a canister kit YSI 6109 For all EXO sondes we strongly recommend the 6109 canister kit The 6109 desiccant canister contains a larger amount of desiccant and is intended for long term d
85. elow for the most reliable performance from the HH This section will identify storage as long term or short term Long term denotes storage during times of long inactivity over winter end of monitoring season etc Short term denotes storage during times the sonde will be used at a regular interval daily weekly biweekly etc 1 Clean handheld If the HH s USB connector is contaminated rinse it with clean water and dry it To clean the HH cable connector follow directions in section 6 24 for Connector Maintenance Wipe clean the HH s keypad lens and polymer case with a cloth soaked in clean water and a few drops of a dishwashing liquid that contains a degreaser Take care not to scratch the lens 2 Short term storage Keep the HH in a safe storage location and power it down by pressing and holding the power button for more than three seconds Pressing the power button for less than three seconds does not entirely power down the instrument Sleep mode and may cause unnecessary battery drain 3 Long term storage Keep the HH in a safe location and remove the batteries and reinstall the battery compartment panel to prevent potentially harmful battery leaks NOTE If the HH is stored for more than several days without batteries the GPS will take longer to obtain a location fix pg 112 Handheld Install or Replace Batteries The EXO Handheld HHs uses four 4 C cell alkaline batteries as a powe
86. ely 50 of the capacity If at any time the battery becomes damaged hot or begins to balloon or swell discontinue charging or discharging immediately Quickly and safely disconnect the charger Then place the battery and or charger in a safe open area way from flammable materials After one hour of observation remove the battery from service DO NOT continue to handle attempt to use or ship the battery Failure to follow these procedures can cause damage to the battery personal property or cause serious injury AN Damaged or swollen batteries can be unstable and very hot DO NOT touch batteries until they have cooled In the event of a fire use a Class A B or C fire extinguisher DO NOT use water If the internal battery fluid comes into contact with your skin wash the affected area s with soap and water immediately If it comes into contact with your eye s flush them with generous amounts of water for 15 minutes and seek immediate medical attention pg 144 8 2 Radio Frequency Xylem certifies that the EXO product line has been tested and complies with the following radio frequency RF interference standards and are approved for use in the following countries e United States FCC Part 15 compliant e Canada RSS compliant e European Union EU CE compliant e Australia CISPR 11 compliant e New Zealand CISPR 11 compliant e Republic of Korea Radio Waves Act compliant e People s Republic of China Radio regulation
87. en Phthalate Red food coloring pH 7 Ingredients Water Potassium Phosphate Monobasic Sodium Hydroxide Yellow food coloring pH 10 Ingredients Water Potassium Hydroxide Disodium EDTA dihydrate Potassium Borate Potassium Carbonate Bromphenol Blue Sodium Salt Bromphenol Green Sodium Salt Inhalation Avoid breathing vapors or mists Inhalation of dust may cause irritation of respiratory tissues Ensure adequate ventilation is available before handling Skin Exposure may cause irritation with repeated exposure Wear rubber or neoprene gloves Eyes Can cause irritation and potential eye damage with repeated exposure Wear safety glasses with side shields or face shield Contact lenses should not be worn when working with these solutions Ingestion May cause nausea vomiting or diarrhea Wear a mouth cover or face shield when there is splashing Do not swallow Do not induce vomiting First Aid See box on page 123 Zobell Solution 3682 Ingredients Potassium Chloride Potassium Ferrocyanide Trihydrate Potassium Ferricyanide Inhalation Inhalation of dust may cause irritation of respiratory tissues Ensure adequate ventilation is available before handling Skin Exposure may cause irritation Wear lightweight protective clothing gloves boots and apron Eyes May cause irritation Wear safety glasses with side shields or face shield Ingestion May cause an upset stomach Wear a mouth cover or face shield when the
88. ensation EXO sensors have internal thermistors for quality assurance purposes Turbidity uses the internal thermistor for temperature compensation while all other EXO sensors reference the C T probe for temperature compensation To display and log temperature a C T probe must be installed in an EXO sonde Thermistor readings are logged in the sonde s raw data viewable in KOR software but are not included in data exported to Excel Conductivity This is a measurement of water conductance from the drive and sense electrodes on the conductivity electrode The output is in mS cm or uS cm Note that the conductivity of solutions of ionic species is highly dependent on temperature and the conductivity output is NOT compensated for temperature Specific Conductivity When Specific Conductance is selected the sonde uses the temperature and raw conductivity values associated with each determination to generate a specific conductance value compensated to 25e0C by default Both the Temperature Coefficient and reference temperature can be adjusted in the advanced sensor menu under calibration nLF Conductivity The non linear function nLF is defined by the ISO 7888 standard and is applicable for the temperature compensation of electrolytic conductivity of natural waters This convention is typically used in German markets Salinity Salinity is determined automatically from the sonde conductivity and temperature readings according to algorithms fo
89. ensive module periodically and not the more costly base The connection of the module to the sensor base is designed for one connection only and the procedure must be conducted in an indoor and dry environment Once installed the module cannot be removed until you are prepared to replace it with a new module See section 6 21 for detailed instructions Users must order either a pH or pH ORP sensor Once ordered the sensor is only compatible with like model sensor modules For example if a pH sensor is purchased initially then the user must order a replaceable pH sensor module in the future it cannot be replaced with a pH ORP module Electrodes EXO measures pH with two electrodes combined in the same probe one for hydrogen ions and one as a reference The sensor is a glass bulb filled with a solution of stable pH usually 7 and the inside of the glass surface experiences constant binding of Ht ions The outside of the bulb is exposed to the sample where the concentration of hydrogen ions varies The resulting differential creates a potential read by the meter versus the stable potential of the reference The ORP of the media is measured by the difference in potential between an electrode which is relatively chemically inert and a reference electrode The ORP sensor consists of a platinum button found on the tip of the probe The potential associated with this metal is read versus the Ag AgCl reference electrode of the combination sensor that util
90. eployments can last up to 1 year in severe conditions It also contains mounting brackets for mounting the canister to a nearby structure The smaller 6108 kit requires replacement frequently in high humidity environments A A desiccant or a connector dummy plug must always be attached to the sonde and cable to prevent moisture from entering into the vent tubes Users must also ensure that the desiccant always remains active Active desiccant is a blue color and when it can absorb no more moisture it is a pink color The end that is vented to the atmosphere will Cables and Desiccants begin to change color first As long as the desiccant closest to the sonde is blue no maintenance is required Local conditions will dictate how long the desiccant will last In humid environments the desiccant may need to be changed or regenerated before it is completely exhausted to ensure that it lasts the entire deployment Mounting Fitting Installing YSI 6109 Desiccant Canister e Remove the 1 8 NPT plugs from the stainless steel fittings on the canister e Install the 1 8 NPT to 1 8 hose fittings into the stainless steel fittings located on the side of the desiccant canister Do not over tighten Place the plugs over the fittings on the canister until you are ready to use the canister e Using suitable screws fasten the canister mounting brackets to an appropriate support structure The spacing between the brackets mus
91. equired in the future perform another dilution of the concentrated solution Effect of temperature on fluorescence The intensity of the fluorescence of many dyes shows an inverse relationship with temperature This effect must be accounted for when calibrating the EXO fDOM sensor with Quinine Sulfate Solution Enter the QSU or RFU value from the table below that corresponds to the temperature of the standard Temp C RFU 30 96 4 28 97 3 26 98 2 24 99 1 22 100 20 100 9 rg 99 Rhodamine WT Dye Solution for Total Algae Sensor A Read and follow all the safety instructions and MSDS documentation supplied with the dye before proceeding Remember that only trained personnel should handle chemicals Preparation Use the following procedure to prepare a Rhodamine WT solution for use as a sensor stability check reagent for the EXO Total Algae Chlorophyll and Blue green Algae sensor 1 Purchase Rhodamine WT dye in solution form which can vary somewhat in nominal concentration Recommended supplier for a solution that is approximately 2 5 in Rhodamine WT Fluorescent FWT Red Dye item 106023 Kingscote Chemicals 3334 South Tech Blvd Miamisburg OH 45342 USA 1 800 394 0678 Accurately transfer 5 0 mL of the Rhodamine WT solution into a 1000 mL volumetric flask Fill the flask to the volumetric mark with deionized or distilled water and mix well to produce a solution that is approximately 125 mg L of Rhodamine WT
92. ering species thus include other halide ions fluoride bromide and iodide as well as other anions Despite the potential problems with interference when using ISEs it is important to remember that almost all interfering species produce an artificially high reading Thus if the sensor indicates the presence of only small quantities it is unlikely that the reading is erroneously low because of interference Unusually high readings which could be due to interfering ions should be confirmed by laboratory analysis after collection of water samples pg 98 Calibration Calibration Standards Quinine Sulfate Solution for fDOM Sensor A Before using a quinine sulfate reagent solid or solution or sulfuric acid reagent read the safety instructions provided by the supplier Take extra precautions when making dilutions of concentrated sulfuric acid as this reagent is particularly dangerous Remember that only trained personnel should handle chemicals Preparation Use the following procedure to prepare a 300 ug L solution of quinine sulfate 300 QSU that can be used to calibrate the EXO fDOM sensor for field use 1 Purchase solid quinine sulfate dihydrate with a high purity gt 99 Recommended supplier Fisher Scientific item 6119 70 6 Purchase 0 1 N 0 05 M sulfuric acid to avoid the hazards of diluting concentrated sulfuric acid to make this reagent Recommended supplier Fisher Scientific item AA35651K7 2 Weigh 0 100 g of so
93. escence sensor and use the default sensitivity for calculation of chlorophyll concentration in ug L allowing quick and easy fluorescence measurements that are only semi quantitative with regard to chlorophyll However the readings will reflect changes in chlorophyll from site to site or over time at a single site Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water In the Calibrate menu select BGA PC Chlor then select Chl ug L Select either a 1 or 2 point calibration Enter 0 for first standard value and 66 for second standard value Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to ret
94. ew one in the same location Confirm that the new guard is aligned with the 4 pin connector at the bottom of the probe as shown and properly centered between ports 1 and 6 after the wiper has been installed in the sonde 6 23 Field Cable Maintenance and Storage EXO field cables are rugged and provide years of reliable service when properly maintained As with all field cables they are most vulnerable at their connectors Take extra caution to protect the connectors from debris and physical harm 1 Inspect and clean cables Inspect the cable s connectors for contamination and remove any detected debris with a blast of compressed air Users should also apply a thin coat of Krytox grease to the male pins of the connectors when they appear dried out However it is better to apply too little grease than too much Too much grease can encourage contamination Periodically inspect the cable for nicks and tears to ensure best performance 2 Cable storage Users should leave the cable installed on the sonde to protect the connectors If necessary users may remove it from the sonde but extra care should be taken to protect the connectors Store the cable in a safe location free from direct sunlight If the cable is vented ensure the storage cap is affixed to the desiccant inlet Store vented cables in a bag containing desiccant pg 131 Connectors Maintenance and Storage EXO sondes utilize wet mate connectors that greatly red
95. ewdriver remove the small rubber plug from the gap in the hard plastic ring at the base of the sensor module A Caution Always exercise extra care when using sharp or potentially harmful instruments 4 7 Vo B 2 Remove and discard old sensor module To remove perform two motions simultaneously 1 With your fingers squeeze the sensor module s hard plastic ring so that it compresses the gap left by the rubber plug 2 Steadily pull the sensor module straight back from the sensor body rocking slightly if necessary i i NOTE The act of removing the old sensor module renders the o rings on the module unusable To prevent catastrophic leaks do not attempt to reinstall a module with damaged o rings Discard the module according to your organization s guidelines or return it to manufacturer for recycling 3 Inspect and service connector cavity Inspect the connector cavity of the probe body for debris or moisture If detected remove it with a lint free cloth or a gentle blast of compressed air p A E _ D 4 Inspect and service new sensor module s o rings Ensure that the two o rings are not nicked or torn and have no contaminants or particles on them If the user detects damage carefully replace them with the extras included in the sensor module kit Then apply a thin coat of Krytox lubricant to each o ring If a user removes a sensor module that is in goo
96. for proper installation and removal of the protective sleeves 1 Fitting the probe sleeves a Prior to installation ensure all probes are detached from the sonde body b Insert a probe into a protective sleeve Line up the edge of the plastic sleeve with the bottom edge of the probe housing nearest the connector then use a marker to note where the sleeve begins to overhang the top edge of the probe head e For C T indicate two lines above and below the vent hole to ensure its exposure during deployment Remove the sleeve from the probe and cut it at the marked area s to remove excess plastic Once appropriately sized slide the anti fouling sleeve back onto the probe e For C T slide both pieces of the sleeve back onto the probe and ensure the vent hole remains exposed a d Repeat this process with all probes except the EXO central wiper see next instructions 2 Fitting the EXO2 central wiper sleeve The central wiper sleeve is comprised of two pieces a Loosen the set screw with a 0 05 in Allen wrench and remove the wiper brush assembly remove dnd c t b Slide the large central wiper sleeve onto the wiper housing at the brush end until it fits snugly into the brush guard The sleeve can be slipped between the guard and the housing by gently pulling the brush guard away from the housing Use a marker to note where the sleeve begins to overhang the edge of the wiper h
97. for serial dilution of Rhodamine WT solution from 0 400 pg L Chl equivalents BGAPC R250 999 for serial i dilution of Rhodamine WT i solution from 0 100 pg L PC equivalents BGA PE R2 gt 0 999 i for serial dilution of Rhodamine WT solution from 0 280 pg L PE equivalents Linearity Optics Chl Excitation 470 PC Excitation 590 Emission 685 20 nm Pigment concentration ranges of algae sensors were determined in monocultures of specific algae species This range will vary depending on algae assemblage and environmental conditions For accurate pigment concentration estimates at particular sites or samples the user must determine the RFU to pigment concentration relationship on a site by site basis pee 27 Although blue green algae contain chlorophyll a the chlorophyll fluorescence signal detected by in situ fluorometers is weaker than in eukaryotic phytoplankton This results in an underestimate of algae biomass when using a single channel chlorophyll sensor when blue green algae are present The EXO total algae sensor generates a more accurate total biomass estimate of the planktonic autotrophic community by exciting chlorophyll a phycocyanin or phycoerythrin The sensor generates data in three formats RAW RFU and an estimate of the pigment concentration in ug L The RAW value is a value unaffected by user calibrations and provides a range from 0 100 representing the per cent of full scale that
98. g applications to ensure the sonde is lowered to the desired depth without waiting for the Depth data to stabilize Fut Be Feet pg 13 Confirm averaging settings To quickly check a sonde s data filtering options examine the summary information at the top of either the desktop or handheld versions of KOR On the desktop software the word Default Accelerated or Rapid will be adjacent to the na5 17 AN Baa D 7 SMD sonde s serial number Similarly on the handheld the letter K gt r EA Z D A or R will be listed at the top right of the screen 13A500523 Defautt lt EXO1 Sonde S vY Chattahooche River Site2 Adaptive Filtering The drawback to a basic rolling filter is that response time to an impulse event is delayed and the more entries in the average summation the longer the delay for the result to converge on the true value To correct this the filter algorithm monitors the new data arriving and compares it to the current averaged result looking for indication of an impulse event When new data deviates from the average by more than a predetermined tolerance the number of data entries within the rolling average is reduced to a minimum count and the remaining values are flushed with the new data The result is a snap to the new value entirely eliminating the inherent delay caused by the rolling average Outlier Rejection Every time a newly acquired data value is added
99. he corresponding sensor installed in order to plot the data File Mode When using the Capture Data button data will be saved to a file When the file mode is set to NEW a new data file will be created Log Mode Controls the amount of data saved to a file Cached sends the entire real time data buffer up to 1000 points in desktop KOR while Single records the most recent data set 1 point for every parameter Sample Interval This option corresponds to the refresh rate of the graph and dashboard 1 sample per second 1 Hz is typical for most real time display once per second new readings are posted to the buffer and updated on the display The sampling rate on the real time display is limited to 4 samples per second 4 Hz because of the processing effort of transferring the information to the PC File Prefix This prefix is the file name that will appear at the beginning of the captured data file KOR automatically applies a unique identifier name on all files generated The user can change this setting but the file prefix allows users to give the captured file a name Graph Type Choose between Time Series or Profiling graph displays The Profiling graph displays data versus depth Default View The Default View allows the user to choose between Dashboard or Graph as the default display when KOR automatically opens the Run menu Apply When a field is edited in the Settings menu the user must click the Apply butto
100. he manufacturer or reseller AA Oo a E e l siete ne A at perforation for EXO2 i D T 1 1 S A x t s P b i Insert plastic tray and cable s on top of items shown here Handheld Sensors Parts kits amp USB drive EXO2 or EXO1 top foam tray bottom foam tray instruction sheets KOR software spare guard spare guard pg 136 Vented Level Sonde 7 1 Overy verview Transducer Transducer Like EXO depth sensors level sensors use a differential transducer with one side exposed to the water However unlike the depth sensors which have their back side sealed in a vacuum the other side of the level transducer is vented to the atmosphere Because of this venting to the surface the transducer will only measure the water pressure exerted by the water column Thus the vented level option for depth measurement eliminates errors due to changes in barometric pressure because the barometric pressure is being seen on both sides of the pressure sensor This is accomplished by using a special sensor that has been vented to the outside atmosphere by way of a tube that runs through the sonde and cable This tube must remain open and vented to the outside atmosphe
101. head Replace the isolation flap over the batteries Do not use 3 6V Li batteries in the sondes Damage to the circuit board is not covered under warranty pg 105 A Check and service o rings AN Before replacing the battery cover check and service the four o rings Ensure that the o rings are not nicked or torn and that they have no contaminants or particles on them or the sealing surfaces inside the battery cover Clean the o rings with a lint free cloth Then apply a thin coat of Krytox lubricant to each o ring 5 Replace battery cover Twist the battery cover clockwise until it stops at the rubber gasket The gasket does not provide a seal and does not need to be compressed Do not overtighten overtightening will not create a strong seal and may damage the sonde The EXO1 sonde has a resealing battery relief valve no maintenance is required If a battery failure occurs that results in battery acid leakage into the battery compartment the sonde must be returned to a service center for evaluation Some battery acid will damage the plastic in the battery compartment Battery life specification When using alkaline batteries Approximately 90 days at 20 C at a 15 minute logging interval and temperature conductivity pH ORP Optical DO and turbidity installed Battery life is heavily dependent on sensor configuration and is given for a typical sensor ensemble Battery life is reduced in cold water applications
102. hwashing liquid that contains a degreaser Rinse the guard with clean water and inspect 2 Soak to remove heavy biofouling Remove the antifouling sonde guard from the sonde If the guard is covered in a thick layer of filaments or barnacles soak the guard for 10 15 minutes in a solution of clean water and a few drops of a dishwashing liquid that contains a degreaser Following the soak rinse the guard with clean water and inspect 3 Scrub to remove heavy biofouling If biofouling remains use a small plastic scrub brush or plastic scraper to gently scrub the biofouling off the guard Then wipe the guard with a wet soapy cloth and rinse Do not sand or polish the inside of the guard bottom as this may effect turbidity readings The guard bottom has a black coating that will eventually wear off pg 134 Flow Cell Maintenance There are two versions of the EXO flow cell EXO1 flow cell 599080 and EXO2 flow cell 599201 Flow rate of the flow cell is typically between 100 mL and 1 L per minute Maximum flow rate depends on tubing type size and length Maximum pressure for each is 25 psi gt 1 Disassemble flow cell To clean the flow cell after use unscrew and remove the t sonde from the flow cell Take apart the flow cell by unscrewing the base from the locking ring Remove the flow cell tube by gently pulling the base and the tube apart The locking ring will remain on the tube due to the stainless steel retain
103. ick 1 Point for the Calibration Points Enter 0 or go to the Advanced menu to enter a known sensor offset If a depth offset is entered the output value will shift by the value of the offset Users may use an offset if referencing a water elevation against a known datum Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point This process zeros the sensor with regard to current barometric pressure Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu For best performance of depth measurements users should ensure that the orientation of the sonde remains constant while taking readings This is especially important for vented level measurements Keep the sonde still and in one position while calibrating Advanced Mounting Use the Advanced menu to select if a sonde will Mountings Fj Fed Depth be mounted in a moving profiling deployment instead of a fixed location Altitude 82 089 Latitude 36 456 Depth Offset Enter a datum or barometric pressure offset at time of calibration Barometric pressure offset allows the depth data to be post processed for barometric pressure C Restore Calibration Defaults changes over the course of the deployment V Check depth settings on Deployment
104. ied Safety Warnings and Precautions sheet included with the battery pack or section 8 1 in this document Battery Pack Disposal For information on how to prepare your battery for disposal please reference the supplied Safety Warnings and Precautions sheet included with the battery pack or section 8 6 in this document pg 53 3 3 Power On Off Handheld Users can power on off and awaken put to sleep the EXO Handheld HH depending on use In order to use the HH users must first power it on When finished with the HH users should power it off to increase its battery life When temporarily not using the HH users should put it to sleep and awaken it when next needed The HH s sleep mode is a low power mode designed to increase its battery life When finished with the HH for a long period of time users should power it off and remove the batteries og 54 1 Power on awaken handheld With batteries installed press and hold the power button for one second The Bluetooth wireless indicator will then illuminate Next the splash screen will appear then briefly go black approximately 5 seconds while the system starts up The HH will then automatically start Kor If the HH is asleep users should briefly press the power button to awaken it Power off put to sleep To power off the HH users must press and hold the power button for more than three seconds Once the button has been held long enough the screen will power down an
105. iles have been copied to the PC users can delete selected files Select the files to be removed and use the Delete Selected button to remove them permanently from the sonde Quick View Click a file in the list and then click the Quick View button to view the last 50 data points of the file Storage space The progress bar on the bottom will indicate memory usage on the sonde We recommend users keep a back up copy of the binary files on the sonde unless storage space is needed View export Use this submenu to review binary files transferred from the sonde and export the binary data to different format View Select and open a saved file from the Data Files pop up menu Alternatively click the Select File button to manually open a file When a file is loaded in desktop KOR you can view it one point at a time using the arrow buttons or change to a graph view using the Change View button Handheld KOR is limited to viewing 100 data points Export Click the Export Data button to export files to Excel format or delimited text file For Excel format a spreadsheet automatically opens with the data Save other open files in Excel first or the export will not work Settings This submenu allows the user to set the default file location export format and data display formatting View calibration worksheets This submenu in desktop KOR allows the user to open and view saved Calibration Worksheets from the Calibration Fi
106. in public low voltage supply systems for equipment with rated current lt 16A per phase and not subject to conditional connection e EN 300 328 V1 7 1 Electromagnetic compatibility and Radio spectrum Matters ERM Wideband transmission systems Data transmission equipment operating in the 2 4 GHz ISM band and using wide band modulation techniques Harmonized EN covering essential requirements under article 3 2 of the R amp TTE Directive e EN 301 489 1 V1 8 1 Electromagnetic compatibility and Radio spectrum Matters ERM Electromagnetic Compatibility EMC standard for radio equipment and services Part 1 Common technical requirements e EN 301 489 17 V2 1 1 Electromagnetic compatibility and Radio spectrum Matters ERM Electromag netic Compatibility EMC standard for radio equipment Part 17 Specific conditions for Broadband Data Transmission Systems e EN 60950 1 Information technology equipment Safety Part 1 General requirements hill Lisa M Abel Director Quality June 7 2012 pg 147 The undersigned hereby declares that the products listed below conform with the Australian and New Zealand Electromagnetic Compatibility EMC requirements for generic products to be used in residential commercial and light industrial environments and carry the C Tick mark accordingly Manufacturer YSI Incorporated a Xylem brand 1725 Brannum Lane Yellow Springs OH 45387 USA Equipment name EXO Sondes EXO1 and EXO2 and EXO
107. ing ring Repeat the same steps to remove the top of the flow cell from the flow cell tube 2 Clean flow cell Use water and a mild detergent and water to wipe clean the flow cell parts 3 Reassemble flow cell Make sure that the o rings and threads are clean and free of any particles such as sand grit or debris Apply a thin coat of Krytox grease to the two o rings on the flow cell tube Make sure that the o rings and stainless steel retaining rings are properly seated on the flow cell tube Push the base of the flow cell onto the flow cell tube until it is firmly seated This creates the watertight seal Screw the locking ring on to the base by turning it clockwise do not use a tool and do not overtighten C P C Repeat same steps to reconnect the top of the flow cell to A the flow cell tube pg 135 Storage Cases 6 27 Packing Equipment In addition to the maintenance procedures for individual components of the EXO system detailed in this section users can store all EXO components sonde sensors handheld cables etc in a large hard sided carrying case 599593 01 which measures 32x20 inches for safe keeping Proper long term storage helps to ensure proper equipment operation Long term denotes storage during times of long inactivity over winter end of monitoring season etc Also available from YSI is a soft sided cloth carrying case 605394 We also recommend Pelican 1600 and 1700 cases ordered from t
108. ing sites A new Create Site option exists under the Handheld s Run menu Selecting this option will allow you to generate a new site using the Handheld s alpha numeric keypad NOTE To tag files with site information or to delete sites on the Handheld you must be connected to an EXO sonde Tip If entering a longer list of sites into the handheld it is easier to utilize KOR Desktops equivalent feature When creating files on the desktop be sure to sync with the handheld 2 Tagging sites to sampling data On the Capture Data menu Run gt Dashboard gt Capture Data all files will be tagged with whichever site is active while you record data To change the active site press 6 on the Handheld keypad and select any of the sites you have created Your active site will be shown on the rotating header Deleting sites To delete a site on the Handheld go to the Change active site menu select the site you wish to delete and press the tab button on the keypad Tip You can also create delete sites on desktop KOR Sites menu and then sync this list with your Handheld Options gt Sync With Handheld This is helpful when generating longer site lists pg 59 3 9 from Handheld to PC The EXO Handheld stores two different sets of sensor data files Files uploaded from the sonde and files manually logged into the Handheld from the live data mode Both types of files can be sent to a PC via the USB cable Note that KOR Desktop soft
109. ious cap has exhausted its usable life typically about one year The DO sensor cap 599110 01 is shipped in a humidified container and should be stored in a 100 humid environment If the sensor cap dries completely follow instructions to rehydrate it 1 A pel 122 Remove current sensor cap Rotate the sensor cap with your fingers counterclockwise until free If possible do not use any tools during this process However should the cap be immovable after use carefully twist the sensor cap with pliers until it breaks loose Do not use pliers on the sensor body and take great care not to damage the sensor threads Replace o ring Without using tools remove the previous o ring pinch the o ring out then roll it upwards over the threads and discard it Visually inspect the new o ring for nicks tears contaminants or particles discard damaged o rings Without twisting it carefully install the new o ring over the threads and into its groove then apply a thin coat of Krytox lubricant to the o ring only Ensure the sensor cap s cavity is completely dry before installing the new cap Install new sensor cap After the o ring is installed and lubricated wipe the clear window at end of sensor with a lint free cloth until clean Then dry the inside cavity of the sensor cap with a lint free cloth With a clockwise motion thread the new sensor cap onto the sensor until it is finger tight The o ring should now be co
110. ipe the sensors prior to every sampling interval In this case you can set the wiping interval to 4 in which case it will wipe every fourth time a sample is taken or approximately every 20 minutes This action can help conserve battery life Setting Samples Per Wipe to 0 will disable the central wiper Sampling Rates The sensors output data to the EXO sonde in real time this data transfer rate varies by sensor and processing conditions but generally it can be assumed the sensor transfers data to the sonde twice a second 2 Hz In high speed unattended sampling applications like vertical profiling the sonde can be deployed to log data as fast as 4 times a second 4 Hz The user can set the sampling interval and the real time transfer between the sensors and sonde will automatically adjust to an appropriate output rate The user can not manually control the output rate of the sensors themselves RS 232 output via SOA DCP is limited to 1 Hz Battery Consumption Sampling rates can vary significantly depending on application and a sampling rate will have a significant impact on memory usage and battery consumption An EXO2 sonde with a full payload can be expected to log more than 90 days at a 30 minute sampling interval but the same sonde set to profile at 4 Hz four times per second will have battery power for only one day An estimate of this life is provided in the deployment summary screen and should be considered when setting sampling inte
111. izes gelled electrolyte ORP values are presented in millivolts and are not compensated for temperature Signal Quality Signal conditioning electronics within the pH sensor module improve response increase stability and reduce proximal interference during calibration Amplification buffering in the sensor head is used to eliminate any issue of humidity in the front end circuitry and reduce noise al 26 Total Algae Chl amp BGA Sensor Overview The EXO total algae sensor is a dual channel fluorescence sensor that generates two independent data sets one resulting from a blue excitation beam that directly excites the chlorophyll a molecule present in all photosynthetic cells and a second from an orange excitation beam that excites the phycocyanin accessory pigment found in blue green algae cyanobacteria This orange excitation triggers a transfer of energy from the phycocyanin to the central chlorophyll a where photosynthesis is initiated 599102 01 Phycocyanin 599103 01 Phycoerythrin continued Specifications Units Chlorophyll RFU pg L Chl BGA PC RFU pg LPC BGAPE RFU pg L PE Temperature Operating 5 to 50 C Storage 20 to 80 C Chl 0 100 RFU 0 400 pg L Chl BGA PC 0 100 RFU 0 100 pg L BGAPE 0 100 RFU 0 280 pg L Chl 0 01 RFU 0 01 pg L Chl Resolution BGAPC 0 01 RFU 0 01 pg L BGA PE 0 01 RFU 0 01 pg L Sensor Type Optical fluorescence Chl R2 gt 0 999
112. l KORHH al 114 611 Handheld Update Sonde Firmware To update the instrument firmware when a sonde is in the field you can use the EXO Handheld instead of a computer 1 Download updates to handheld Connect the Handheld to a computer with internet access to retrieve the latest firmware updates see section 6 10 In Selected Device KOR desktop software go to the Options menu select Sync sn 11N502308 with Handheld and follow the prompts When complete Address 18328100 your Handheld can be brought to the field to deliver ete rN Biel zos updates to sondes and sensors Turbidity 6 t73 Neu FU 1 0 3 lt empty gt Dep NV 0 250m D 2 Connect handheld to sonde Connect Handheld to sonde via Bluetooth see section 3 5 or field cable see section 3 4 Steve STSO2308 3 Update sonde and sensors When the Handheld is connected to the sonde go to the Options Firmware menu on the Handheld The list on the Turbidity left side displays the connected device s current firmware SenEeye 2 and any new firmware available to install DEEA e To update the sonde and all sensors in the sonde click the Update All soft key e To update an individual sensor select the sensor from the list and click the Toggle Advanced soft key Then select the firmware version from the list on the right and click the Update Selected soft key The firmware update process requires e Click OK to confirm that you would like to stop loymen
113. leakage into battery compartment requires that your instrument be evaluated by the manufacturer or Authorized Service Center before the next deployment Battery Life Specification When using alkaline batteries Approximately 90 days at 20 C at a 15 minute logging interval and temperature conductivity pH ORP Optical DO turbidity and Total Algae PC installed along with a central wiper which rotates once every logging interval Battery life is heavily dependent on sensor configuration and is given for a typical sensor ensemble Battery life is reduced in cold water applications When using rechargeable nickel metal hydride NiMH batteries Estimated battery life is not available because NiMH batteries vary greatly in manufacturer capacity and discharge curves We recommend a NiMH D cell battery with a minimum rating of 10 000 milliamp hours that are fully charged each time they are used pg 108 Sonde Replace EXO Bail Sonde bails provide users with a handle for convenient transport and an attachment point for cable strain reliefs If an EXO1 bail breaks due to impact or standard wear and tear throughout the life of the sonde a user can easily replace it We also recommend attaching the cable s strain relief mechanism to the bail 1 Remove battery cover Twist the battery cover counterclockwise until free Then slide off the battery cover 2 Remove bail Spread the sides of the bail away from the connector pull the bail over
114. les folder on the computer If you encounter errors viewing data files synced from the EXO Handheld click the Update Directory button 4 7 KOR Software Options Menu Many preferences settings and updates for KOR can be accessed through the Options menu Smart QC KOR performs quality checks on each connected sonde and sensor and provides an overall Network QC Score w4 Check mark green OK manan paayos C 9 X red Warning that part or all of the system is out of specification ranges 9 Question mark black Unknown not enough data to determine QC score Exclamation point yellow Caution While OK now part s of the system is close to being out of specification Sonde Bluetooth pin number activate Bluetooth sonde ID name sonde date and time battery type and fault bit field KOR software on the EXO Handheld also has a Handheld submenu to set language date and time power GPS sound volume Bluetooth pin number and hibernate sleep preferences on the Handheld itself User Select settings for Language Idle Timeout and Time Zone and Time Format preferences and turn on off time zone check at deployment Units Customize display units parameters for each sensor plus adjust wiper position and sonde cable and battery readings While the sonde and sensors record data in fixed formats KOR can adjust the displayed units For example the Temperature sensor outputs degrees
115. lick Apply to accept this calibration point Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde Blue green Algae Phycocyanin This procedure calibrates BGA RFU or BGA ug L If the user has both units selected then this procedure must be performed twice once for each unit to completely calibrate the parameter For the 2 point calibration one of the standards must be clear water 0 ug L and this standard must be calibrated first The other standard should be in the range of the suspected BGA PC content at the environmental site Two general types of standards can be used a phytoplankton suspensions of known BGA PC content and b dye solutions whose fluorescence can be correlated to that of BGA PC The user is responsible for determining the BGA PC content of algal suspensions by using standard cell counting techniques For option b we recommend using a 625 ug L Rhodamine WT dye so
116. lid quinine sulfate dihydrate and quantitatively transfer the solid to a 100 mL volumetric flask Dissolve the solid in about 50 mL of 0 05 M 0 1 N sulfuric acid H SO dilute the solution to the mark of the volumetric flask with additional 0 05 M sulfuric acid and mix well by repeated inversion This solution is 1000 ppm in quinine sulfate 0 1 3 Transfer 0 3 mL of the 1000 ppm solution to a 1000 mL volumetric and then fill the flask to the top graduation with 0 05 M sulfuric acid Mix well to obtain a solution of 300 ug L 300 QSU or 100 RFU 4 Store the concentrated standard solution in a darkened glass bottle in a refrigerator to retard decomposition The dilute standard prepared in the previous step should be used within 5 days of preparation and should be discarded immediately after exposure to EXO s metal components Degradation of quinine fluorescence by copper and chloride AN Exposure of the quinine sulfate solution to any copper based component of the EXO sonde and sensors primarily the wiper assembly will begin to degrade the solution significantly within minutes Quinine fluorescence is also degraded by the presence of chloride or halide ions found in estuarine or seawater conductivity standards and Zobell solution Thus clean your sensors thoroughly and perform your calibration as quickly as possible on immersion of the sensors into the quinine sulfate solution Discard the used standard When quinine sulfate standards are r
117. lution for detailed instructions see section 5 11 and the solution is used in the calibration steps below ug L l or 2 point This procedure will zero your fluorescence sensor and use the default sensitivity for calculation of phycocyanin containing BGA in ug L allowing quick and easy fluorescence measurements that are only semi quantitative with regard to BGA PC However the readings will reflect changes in BGA PC from site to site or over time at a single site Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water pg 91 In the Calibrate menu select BGA PC Chlor then select BGA ug L Select either a 1 or 2 point calibration Enter 0 for first standard value and 16 for second standard value Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this
118. m 1 of i reading 0 0001 to 0 01 mS cm i range dependent 4 electrode nickel cell Celsius 5 to 50 C 20 to 80 C 1 5 to 35 C 0 01 C 35 to 50 C 0 05 C Thermistor pg 15 Temperature Thermistor The temperature sensor uses a highly stable and aged thermistor with extremely low drift characteristics The thermistor s resistance changes with temperature The measured resistance is then converted to temperature using an algorithm The temperature sensor receives a multi point NIST traceable wet calibration and the accuracy specification of 0 01 C is valid for expected life of the probe No calibration or maintenance of the temperature sensor is required but accuracy checks can be conducted Conductivity Electrodes The conductivity sensor uses four internal pure nickel electrodes to measure solution conductance Two of the electrodes are current driven and two are used to measure the voltage drop The measured voltage drop is then converted into a conductance value in milliSiemens millimhos To convert this value to a conductivity value in milliSiemens per cm mS cm the conductance is multiplied by the cell constant that has units of reciprocal cm cm 1 The cell constant for the conductivity cell is approximately 5 5 cm 10 For most applications the cell constant is automatically determined or confirmed with each deployment of the system when the calibration procedure is followed Temperature Comp
119. m Storage AN It is important that the air in a sonde s vent tube remains dry at all times Level Sensor Storage Users can store these sensors either dry or submerged in clean water However regardless of storage method or length ensure the vent tube remains dry Always attach the port plug to the cable connector or leave the cable installed with a cap over the desiccant s vent Level Desiccant Maintenance Active desiccant is blue saturated desiccant is pink or rose red When the desiccant closest to the sonde begins to turn pink you should replace YSI 6108 or regenerate YSI 6109 the desiccant cartridge To regenerate desiccant remove it from the cartridge and heat it for one hour at 200 C about 400 F then cool it an airtight container before refilling Also heat the felt filters at 100 C about 200 F for 30 minutes The desiccant will turn blue following a successful recharge Connectors Maintenance Connectors on vented level cables have five pins and a vent pin Periodically inspect the connectors for signs of contamination If you detect debris carefully remove it Prior to initial installation or when dry apply a light coat of Krytox grease to the rubber mating surfaces of the connector including the rubber portions of the pins A Do not allow grease to enter or block the vent tube on the cable connector or the vent opening on the sonde connector When not in use always install the sonde and cable d
120. m denotes storage during times the sonde will be used at a regular interval daily weekly biweekly etc 1 Short term storage When in regular field use the ODO sensor should remain installed on the sonde Place approximately 0 5 in 1 cm of any water deionized distilled tap or environmental in the bottom of the calibration cup Insert the sonde and sensor into the cup and screw it on tightly to prevent evaporation More information in Short Term Sonde Storage section 6 1 2 Long term storage Leave the sensor installed in the sonde and submerge it in clean water in the calibration cup Screw the cup on tightly to prevent evaporation Users may also store the ODO sensor by itself in two ways One submerge the sensing end of the sensor in a container of water occasionally check the level of the water to ensure that it does not evaporate Two store the sensor in water saturated air We do not recommend storing the sensor with the connector end unmated or exposed If unmated cover with plastic connector cap pg 120 6 16 Dissolved Oxygen Sensor Maintenance and Rehydration EXO optical Dissolved Oxygen DO sensors require unique maintenance instructions due to their sensing membranes Users should routinely perform these instructions in order to achieve the highest levels of sensor accuracy DO sensor caps have a typical life of 12 months After this point users should replace the DO membrane cap As cap
121. materials that cannot stretch chain wire rope to ensure a fixed location Depth sensors on the EXO2 sondes are not on center In horizontal deployments take care to ensure the redeployments are always in the same orientation Indentation for location or positioning pin to ensure To assist with consistent horizontal orientation the EXO2 sonde consistent horizontal orientation has an indentation at the top of the sonde for a location or positioning pin A Never band clamp a sonde This can lead to the sonde body becoming warped and taking on water EXO Depth Sensor Reference Points see diagram on previous page e From bottom of sensor guard metal or plastic to transducer diaphragm 34 8 cm 13 7 inches e From face of sensor endcap to transducer diaphragm 27 2 cm 10 7 inches e From face of connector bulkhead to transducer diaphragm 13 9 cm 5 5 inches EXO2 Depth Sensor Reference Points see diagram on previous page e From bottom of sensor guard plastic or metal to transducer diaphragm 21 4 cm 8 4 inches e From face of sensor endcap to transducer diaphragm 13 9 cm 5 5 inches e From face of connector bulkhead to transducer diaphragm 0 7 cm 0 3 inches e Horizontally positioned sonde from outer case location pin down to transducer diaphragm 2 1 cm 0 8 inches Ambient Light Interference When deploying horizontally it is best to keep the sonde s optical sensors out of direct sunlight W
122. me a step change in the sensor s primary output parameter is applied and the time to reach 63 of the final stimulus value is recorded Repeated characterization of multiple sensors provides the T63 specification Sensor Accuracy Specifications To maintain accuracy specifications for EXO sensor we recommend that users calibrate sensors in the lab in standards with temperatures as close to the ambient temperature of the field water as possible alIa Conductivity Temperature Sensor Overview The EXO combination conductivity and temperature sensor should be installed in a sonde in nearly all sonde applications Not only will this sensor provide the most accurate and fastest response temperature data but it will also provide the best data for the use in temperature compensation for the other EXO probes The conductivity data is used to calculate salinity non linear function nLF conductivity specific conductance and total dissolved solids and compensate for changes in density of water as a function of temperature and salinity in depth calculations if a depth sensor is installed 59987001 continued Specifications Conductivity Default Units Temperature Operating Storage Sensor Type Temperature Default Units Temperature Operating Storage Sensor Type microSiemens centimeter 5 to 50 C 20 to 80 C 0 100 mS cm 0 5 of i reading or 0 001 mS cm whichever is greater 2 100 200 mS c
123. mperature range of the media being monitored is known and stable 3 point Select the 3 point option to calibrate the ammonium sensor using three calibration standard solutions two at ambient temperature and one at a temperature substantially different from ambient The 3 point calibration method should be used to assure maximum accuracy when the temperature of the media to be monitored cannot be anticipated 3 point calibration temperatures should span the range of interest for example 20 C and 2 C for cold and 20 C and 30 C for hot The procedure for this calibration is the same as for a 2 point calibration but the software will prompt you to place the sensor in the additional calibration standard solution to complete the 3 point procedure Be certain that the calibration standard solution and sensor are thermally equilibrated prior to proceeding with the calibration The recommended order of calibration standards is 1 1 mg L NH N standard at ambient temperature 2 100 mg L NH N standard at ambient temperature and 3 1 mg L NH N standard at a different temperature usually lower than ambient 10 C minimum To save time during calibration chill heat a sufficient amount of 1 mg L NH N calibration standard solution prior to the start of calibration pg 96 Ammonium 3 point Do not expose electrodes to high conductivity solutions Exposure will reduce data quality and response of the sensors During calibratio
124. mpressed between the sensor cap and sensor and not pinched If pinched remove and discard the o ring and repeat procedure A Do not over tighten the sensor cap Do not use any tools for the installation process i 4 Configure sonde for new cap ODO sat In KOR software configure the sonde for the new sensor cap Click the Calibrate button and then click the ODO button Next click the ODO sat button and in the DO calibration window click the Advanced button In the Advanced menu click the Edit button and enter the unique membrane cap coefficients found on the instruction sheet shipped with the DO sensor cap NOTE Calibration coefficients are associated with specific individual sensor caps They cannot be used for other ODO sensors Although measures are taken at the factory to ensure this please check that the serial number with the calibration coefficients on the instruction sheet matches the serial number engraved on the outside of the sensor cap 5 Store sensor cap The sensor cap is shipped in a humidified container and should be consistently stored in a 100 humid environment Prior to installation ensure the cap s container remains moist Once the sensor cap is installed on the sensor maintain this environment by placing approximately 0 5 in 1 cm of water deionized distilled tap or environmental in the bottom of the calibration cup and screw it tightly onto the sonde to prevent evaporation You m
125. n A Never insert solid objects into the sonde ports This could permanently damage the connectors Sonde Install or Replace EXO 1 Batteries EXO1 water quality sondes use two 2 D cell batteries as a power source Using alkaline batteries users can expect approximately 90 days of deployment from a fully loaded sonde that samples once every 15 minutes However deployment times may vary greatly depending on water temperature sampling rate sensor payload and brand of battery See battery life specification next page Do not use Ni Cad rechargeable batteries in the EXO1 sonde 1 Remove battery cover Start with a clean and dry sonde Hold the sonde horizontally with the bail up and twist the battery cover counterclockwise until free If necessary slide the sonde tool s larger opening over the end of the battery compartment and use it as a lever to break the compartment free Then slide off the battery cover A Do not remove the screws on the sonde Do not clamp the sonde in a vise 2 Remove old batteries Expose the batteries by flipping the isolation flap up away from the batteries and pull the batteries free of their compartment Always dispose of used alkaline batteries according to local requirements and regulations Clean the inside of the battery compartment with a lint free cloth 3 Install new batteries Install the new batteries so that the positive terminals point towards the bail away from the sensor bulk
126. n a turbidity signal The EXO Turbidity sensor employs a near infrared light source and detects scattering at 90 degrees of the 599101 01 incident light beam According to ASTM D7315 method this type of turbidity sensor has been characterized as a nephelometric near IR turbidimeter non ratiometric This method calls for this sensor type to report values in formazin nephelometric units FNU FNU is the default calibration unit for the EXO sensor but users are able to change calibration units to nephelometric turbidity units NTU raw sensor signal RAW or total suspended solids TSS assuming the user enters the appropriate correlation data The RAW value is a value unaffected by user calibrations and provides a range from 0 100 representing the per cent of full scale that the sensor detects in a sample continued Specifications Default Units FNU Temperature Operating iD to 50 C Storage i 20 to 80 C 0 999 FNU 0 3 FNU or i 2 of reading whichever is i greater 1000 4000 FNU 5 i of reading 0 999 FNU 0 01 FNU 1000 4000 FNU 0 1 FNU Sensor Type Optical 90 scatter Optics Excitation 860415 nm ASTM D7315 07a Test Method for Determination of Turbidity Above 1 Turbidity Unit TU in Static Mode pg 29 While all turbidity sensors will read consistently in formazin other calibration solutions and field readings will vary between different models of turbidity sensors
127. n of other sensors remove the ISEs to avoid exposing them to conductivity standards Zobell solution pH buffer or any solution with significant conductivity In the Calibrate menu select ISE then select ammonium Click 3 point for the Calibration Points Enter 1 mg L as the value of the first standard 100 mg L as the value of the second standard and 1 mg L as the value of the third standard Click Start Calibration Pour a sufficient amount of 1 mg L NH N calibration standard solution at ambient temperature in a clean and dry or pre rinsed calibration cup Carefully immerse the sensor end of the sonde into the solution making sure the sensor s tip is in solution by at least 1 cm Allow at least 1 minute for temperature equilibration before proceeding Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Confirm that the Pending data value is close to the Setpoint value Click Proceed and wait for the software to prompt you to move the sensor to the next calibration standard solution Rinse the sensors in deionized water between changes of the calibration solutions Pour a sufficient amount of 100 mg L of NH N calibration standard solution at ambient temperature into a clean dry or pre rinsed calibration cup and carefully immerse the sensor end of the sonde into the solution Allow at lea
128. n to commit those changes If you edit a field then attempt to navigate away a warning box appears to remind you to apply or discard your changes Display Sonde Time Will add a new clock to dashboard with real time clock from sonde time will be displayed in UTC regardless of time zone settings pg 65 KOR Software Calibrate Menu This menu is the main interface for calibrating EXO sensors Calibration and verification settings will vary by sensor A device must be connected to access the Calibrate menu For detailed calibration procedures for individual sensors reference Section 5 Calibration Po e OSAP SO Calibration menu overview p In the Calibrate menu the list of installed sensors is shown on the left side of the window If your sonde is configured with a depth sensor Port D depth also appears in the list P Sensor calibration menu Select a sensor from the list to bring up a sensor specific menu of parameters For example selecting ODO optical dissolved oxygen from the list brings up a menu for the enabled parameters ODO sat ODO mg L or ODO Local To change the enabled parameters on desktop KOR go to the Options Units menu Handheld KOR calibrates in base units regardless of user selections made in the Units menu A typical calibration window shows 1 2 or 3 calibration points depending on the sensor If the sensor supports only a single calibration point then the other calibration
129. ndard Ultraviolet Light DOM Sensor Lithium Ion Battery Handling 8 2 Radio Frequency 8 3 Declarations of Conformity 8 4 Instrument Warranty 8 5 Instrument Cleaning and Packing Instructions 8 6 Recycling Batteries Rechargeable Li Battery Pack pg 4 EXO 1 Sonde Overview The EXO1 sonde is a multiparameter instrument that collects water quality data The sonde collects the data with up to four user replaceable sensors and an integral pressure transducer Each sensor measures its parameter via a variety of electrochemical optical or physical detection methods Each port accepts any EXO sensor and automatically recognizes its type Depending on user defined settings the EXO1 will collect data and store it onboard the sonde transfer the data to a data collection platform DCP or relay it directly to a user s PC or EXO Handheld See section 7 for information specific to vented level sondes Users communicate with the sonde via a field cable to an EXO Handheld Bluetooth wireless connection to a PC or EXO Handheld or a USB connection via communications adapter to a PC Specifications Operating Environment Depth Rating 250 meters 820 feet Xenoy lexan bronze i titanium 316 stainless Internal Logging Memory Capacity Universal Sensor Ports Kor Interface Software Communications NOTE Starting early 2014 EXO sonde bulkhead material changed from bronze to titanium Sonde USB RS 485 U
130. ning the Desktop version of KOR and the computer must have internet access This process may take up to 30 minutes depending on the number of sensors updated Update Firmware Updating pH SN 11P000314 firmware to version 1 0 10 Loading Application Section of Code Please Wait Open firmware submenu in KOR Navigate to the Options menu in KOR then to the Firmware submenu Immediately after clicking the Firmware submenu button KOR begins to search for connected sondes and sensors and loads the table with names serial numbers and current firmware versions Select device and update To update a device click on the devices name in the table and then click the Update Selected button KOR then updates the device s firmware which could take several minutes To update all connected devices i e attached sonde and senors click the Update Network button NOTE For best power management update firmware while a device is connected via USB as this will provide power to the device However if you use Bluetooth we recommend installing fully charged batteries in the sonde See section 6 11 for instructions on how to update sonde and sensor firmware using the EXO Handheld when the sonde is in the field pg 111 6 8 Handheld Maintenance and Storage EXO Handhelds HH are rugged field instruments that are tested to a rating of IP 67 in the factory Follow the instructions b
131. ntentional radiators Manufacturer Equipment name Model numbers Intentional Radiators CMIIT ID Regulation YSI Incorporated a Xylem brand 1725 Brannum Lane Yellow Springs OH 45387 USA EXO Sondes EXO1 and EXO2 and EXO Handheld Systems 599501 xx 599511 xx 599502 xx 599512 xx 599150 EXO Sondes EXO1 and EXO2 contain the LMX Bluetooth module EXO Handheld 599150 contains a Wi Fi Bluetooth module CMIIT ID 2012DJ7503 EXO1 CMIUT ID 2012DJ7504 EXO2 CMIIT ID 2012DJ7505 EXO Handheld Radio Regulations of the People s Republic of China ARIE NAA EMAEMA SAME AA MESA SEAT OARH FARIS AR FALEBIMERS Class A device Broadcasting and communication equipment for office work Seller and user shall be noticed that this equipment is suitable for electromagnetic equipment for office work Class A and it can be used outside the home ha ll Lisa M Abel Director Quality December 13 2012 pg 150 The undersigned hereby declares that the products listed below conform with all applicable requirements of TELEC and Radio Law of Japan for intentional radiators Manufacturer YSI Incorporated a Xylem brand 1725 Brannum Lane Yellow Springs OH 45387 USA Equipment name EXO Sondes EXO1 and EXO2 and EXO Handheld Systems Model numbers 599501 xx 599511 xx 599502 xx 599512 xx 599150 Intentional Radiators Intentional radiators EXO Sondes Exol and Exo2 contain the LMX Bluetooth module Exol
132. nverse relationship with temperature This effect must be accounted for when calibrating the EXO ORP sensor with Zobell solution Enter the mV value from the table below that corresponds to the temperature of the standard pg 87 Calibration Turbidity Before calibrating be certain that the probe is clean and free of debris Solid particles particularly those carried over from past deployments will contaminate the standards during your calibration protocol and cause either calibration errors and or inaccurate field data cleaning instructions section 6 13 Use a clean spare sonde guard Also review the basic calibration description in section 5 1 For proper calibration you must use standards that have been prepared according to details in Standard Methods for the Treatment of Water and Wastewater Section 2130 B Acceptable standards include a formazin prepared according to Standard Methods especially for calibration points greater than 1010 b dilutions of 4000 NTU formazin concentrate purchased from Hach c Hach StablCal standards in various NTU denominations and d AMCO AEPA standards prepared specifically for the EXO turbidity sensor by the manufacturer see table next page A The use of standards other than those mentioned above will result in calibration errors and inaccurate field readings It is important to use the same type of standard for all calpoints i e do not mix formazine and AMCO AEPA standard for diffe
133. o point calibration make certain that the vessel you use has a small exit port to prevent back diffusion of air and that you have completely purged the vessel before confirming the calibration If you use sodium sulfite solution for the zero point calibration prepare the solution at a concentration of approximately 2 g L at least two hours prior to use and keep it sealed in a bottle which does not allow diffusion of oxygen through the sides of the container Transfer the sodium sulfite solution rapidly from its container to the calibration cup fill the cup as full as possible with solution to minimize head space and seal the cup to the sonde to prevent diffusion of air into the vessel Place the sonde with DO and temperature sensors in a zero oxygen medium In the Calibrate menu select ODO then select either ODO sat ODO local or ODO mg L Click 2 Point for the Calibration Points Enter Zero Point as the value of the first standard Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point If you used sodium sulfite solution as your zero calibration medium you must thoroughly remove all traces of the reagent from the probes and wiper prior to proceeding to the second point We recommend that the second calibration point be in air saturated water if you use sodium sulfite sol
134. onde 6 See step 3a to complete the connection in KOR H YSI Sonde 4 Confirm successful link Pastkey enabled Once the device has been added confirm that the device appears in e Win XP Devices tab of the BT Devices window e Win 7 Devices and Printers screen If the device is not listed attempt the establishment process again z This process establishes a secure wireless link between the General Hardware Services Bluetooth fest teens nate sonde and a PC Once established users will not need to This Bluetooth device offers the following services To use a x A service select the check box perform this process again in order to link with the sonde Bluetooth Services F Serial port SPP COMT Ports KOR automatically scans ports for both USB adapters and Bluetooth To view the comm port associated with Bluetooth e Win XP Go to the Bluetooth menu on your computer click Show Bluetooth Devices click on the device you added then click Properties gt Services e Win 7 Go to the Devices and Printers screen right click your sonde then click Properties gt Hardware pg 45 Connect Sonde Data Collection Platform DCP This signal output adapter DCP SOA 599800 allows users to connect an EXO sonde to a Data Collection Platform as well as power it via an external 12 V DC source not included Users wire a sonde cable with flying leads into one side of the SOA and an SDI 12 RS
135. ons Use only the recommended materials to service instruments Each sonde comes with a maintenance kit including proper lubricants and replacement o rings Users can order replacement o ring kits 599680 or 599681 or tool kit 599594 from the manufacturer or an authorized distributor 1 Inspect and service o rings User serviceable o rings are located in the EXO sonde battery compartments Perform a thorough visual inspection of o rings each time they are exposed Carefully look for grit hair etc on the o ring and mating surfaces and wipe away any contamination with a lint free cloth Without removing them from their grooves lightly grease each o ring with Krytox Replace any damaged o rings 2 Replace o rings If the above inspection reveals a damaged split cracked or misshapen o ring remove it Wipe the groove clean with alcohol and a lint free cloth Grease the o ring by drawing it between your lightly greased thumb and index fingers Place the o ring in its groove being careful to not roll or twist it and lightly grease the surface Inspect the o ring for contamination A Do not apply excess grease to the o rings This can cause contamination and seal failure 3 Inspect clean and grease ports Visually inspect each port for contamination grit hair etc Should the user detect contamination remove it with a blast of compressed air When the ports rubber appears dry lightly grease the sensor connector before insertio
136. onsuming 2 point and 1 point routines to update the 3 point calibration However we strongly recommend a new 3 point calibration after each deployment of 30 days or longer Due to the nature of ion selective electrodes it is recommended that they be used for sampling purposes for the greatest accuracy Using an ISE in long term deployments is possible but it s important to note that drift occurs over an extended period of time Collecting grab samples from the site is encouraged to correct for drift Additionally sample readings should be taken after sensors have fully stabilized Calibrating in a continuously stirred solution from 1 to 5 minutes has shown to improve sensor performance For best performance sensors should be calibrated as close to the expected field conditions as possible For more ISE precautions drift and accuracy notes please see ISE Precautions at the end of this section point Select the 1 point option only if you are adjusting a previous calibration If a 2 point or 3 point calibration has been performed previously you can adjust the calibration by carrying out a 1 point calibration 2 point Select the 2 point option to calibrate the ammonium sensor using only two calibration standard solutions In this procedure the ammonium sensor is calibrated using a 1 mg L NH N and 100 mg L NH N calibration standard solutions A 2 point calibration procedure as opposed to a 3 point procedure can save time if the te
137. oth wireless This technology provides a secure two way reliable communication channel with which users can communicate with their sondes above water without cables Many new computers are equipped with Bluetooth wireless installed internally those without Bluetooth can use a Bluetooth dongle not included Follow the manufacturer s instructions for installing the dongle s software and hardware Administrative permissions and IT support may be required depending on the adapter and your PC settings 1 Install Bluetooth dongle optional If your computer is not equipped with internal Bluetooth radio insert a Bluetooth dongle not provided into any of the computer s USB ports Wait for the computer to automatically install the device and its drivers Once the installation is complete the computer should indicate that the device is installed and ready to use e a The preferred Bluetooth configuration is Windows 7 with native Windows Bluetooth drivers and software i 2 Activate sonde s Bluetooth Users activate Bluetooth wireless by holding a magnet at the magnetic activation area In addition to magnetic activation users can also activate Bluetooth by e Applying power to the sonde remove and reinstall batteries See more information on sonde activation and LED conditions in sections 2 7 and 2 8 CSTE 3a Establish Bluetooth link Search Devices Options COM Ports Hardware a cin iowa Bluetooth Win 7 or Win XP
138. ousing mark remove Remove the sleeve from the wiper and cut it at the marked area to remove excess plastic a d Slide the remaining plastic onto the connector end of the wiper until it touches the bottom of the brush guard Use a marker to note where the plastic begins to overhang the wiper housing pg 36 e Remove the sleeve from the probe and cut it at the slide on marked area to remove the excess plastic f Once both pieces of the sleeve are appropriately sized slide them back onto the wiper 3 Fitting the EXO2 sonde sleeve Install all probes and the guard onto the sonde Equip the sonde with a protective sleeve starting at the bail end of the sonde for easiest installation Line up the end of the plastic with the top of the sonde body in yellow y 4 Heat shrinking the sleeves To form fit the plastic to the equipment you must heat shrink the sleeves using one of two methods Heat Gun Hair Dryer Highest Setting Do not hold heat on any one spot for more than one second Move the heat back and forth and continue to rotate the equipment until the plastic clings tightly to it This process should take less than 5 seconds with a heat gun and 15 20 seconds with a hair dryer on its highest setting NOTE If using the heat gun method shrink the sleeves on the probes first install the probes in the sonde and install the guard Then shrink the sonde sleeve Oven
139. ove any debris with a gentle blast of compressed air Prior to initial installation or when dry apply a light coat of Krytox grease to the rubber area of the sensor s connector 4 Replace locking nut If the locking nut near the sensor connector wears out users can replace it with 599668 sensor or 599669 EXO2 central wiper First remove the retaining ring by inserting the tip of a small flat blade screwdriver under the lip of the ring and pry upward Pull ring out of groove Slide off locking nut and replace with new locking nut Install new retaining ring by prying up one edge with screwdriver and fitting it into groove Use the screwdriver to follow the diameter of the ring around the groove to seat it fully Retaining Locking Nut Ring i lt p D N Wear eye protection when servicing the retaining ring pg 133 6 25 Antifouling Equipment Maintenance Many components on EXO sondes are made of an anti fouling copper alloy material that discourages the growth of aquatic organisms However longer deployment intervals and highly productive waters can result in biofouling attachment to the equipment which should be cleaned periodically See also instructions for cleaning individual sensors 1 Remove minimal biofouling Remove the antifouling sonde guard from the sonde If the guard is covered in a thin layer of slime or filaments wipe away the biofouling with a cloth soaked in clean water and a few drops of a dis
140. ownloading the latest firmware please see the Troubleshooting section in this document A NOTE Firmware updates require all instrumentation Handheld sonde sensors and desktop software be updated to function properly pg 79 4 12 Update Firmware and Software Handheld To update the instrument firmware and KOR software on the EXO Handheld use the Desktop version of KOR ona computer with internet access KOR Desktop will go online and pull updated files for the Handheld which are then transferred to the Handheld 1 Connect handheld to computer Plug the small end of the USB cable into the port on the top side of the EXO Handheld Plug the other end of the USB cable into a port on your computer Wait for Windows to recognize the Handheld as a removable drive before the Handheld shows up in KOR software This may take several minutes 2 Update handheld When the Handheld is connected to the PC go to the Options Firmware menu in KOR Desktop software Select the Update Handheld button from the bottom right corner of the menu eeeeee Handheld information will be displayed in this box when successfully connected Follow the prompts for completing the update process and rebooting the Handheld AN NOTE Firmware updates require all instrumentation Handheld sonde sensors and desktop software be updated to function properly pg 80 Calibration Basic EXO sensors except temperature require periodic calibration
141. pH or pH ORP then select pH Select the number of points desired for the calibration Enter the value s of the pH buffer s that will be used for the calibration Observe the temperature reading above the standard value The actual pH value of all buffers varies with temperature Enter the correct value from the bottle label for your calibration temperature for maximum accuracy For example the pH of one manufacturer s pH 7 Buffer is 7 00 at 25 C but 7 02 at 20 C Ifno temperature sensor is installed user can manually update temperature by entering a value Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Confirm that the Pending data value is close to the Setpoint value Click Proceed and wait for the software to prompt you to move the sensor to the next standard solution Rinse the sensor in deionized water Pour the correct amount of an additional pH buffer standard into a clean dry or pre rinsed calibration cup and carefully immerse the probe end of the sonde into the solution Allow at least 1 minute for temperature equilibration before proceeding Repeat the calibration procedure and click Apply when the data are stable Rinse the sensor and pour additional pH buffer if necessary Repeat calibration procedure for the third point and click Apply when data
142. protect against physical damage Align set screw in D shaft Rock brush back and forth 1 Replace wiper brush Loosen set screw with 0 050 inch Allen wrench Remove old brush assembly and clean any residue from wiper shaft and wiper end cap Install new brush assembly gently pressing the wiper arm down against shoulder on wiper shaft Tighten set screw to a torque of 4 inch pounds While tightening gently and slowly rock the brush to ensure a tight fit against the D shaft Check snugness of wiper by gently rocking 5 degrees in either direction 2 Replace brush guard In KOR software go to Run gt Dashboard Click the Wipe Sensors button to ensure proper wiper park position Mark the position of the old guard with a marker Loosen the 6 screw with a 7 64 inch Allen wrench remove the otd guard and clean any residue from motor housing Remove cover on adhesive strip on the inside of the new brush guard Carefully install new brush guard in same position as old guard with brush centered in well Tighten screw until snug but do not overtighten The adhesive helps to hold the guard in place If necessary calibrate the position of the new wiper in the KOR Calibrate menu NOTE The adhesive on the guard strap which facilitates installation may make it difficult to re position the wiper guard after it s been installed Take caution to mark the position of the old guard before removing it and install the n
143. r source Alternatively a rechargeable Li Ion battery pack is an available option see section 3 2 Users can extend battery life by putting the HH in Sleep mode when convenient by pressing and holding the power button for less than three seconds 1 5V Rechargeable Nickel Metal Hydride NiMH batteries can also be used Battery life varies depending on GPS and Bluetooth wireless use We recommend battery capacity of at least 5000 milliamp hours Do not use Ni Cad rechargeable batteries in the Handheld 1 Remove battery cover panel The battery cover panel is located on the back of the HH To remove the panel unscrew counter clockwise the four screws with a flat or Phillips head screwdriver NOTE The retaining screws are captured into this panel and are not independently removable If replacement is necessary replace the entire assembly 2 Insert replace batteries A Do not use 3 6V Li batteries in the handheld Damage to the circuit board is not covered under warranty Remove the old batteries and dispose of them according to local ordinances and regulations Install the new batteries between the battery clips with their polarity oriented as shown on the bottom of the battery compartment If you use your own rechargeable batteries they cannot be charged inside the handheld they should be charged outside the handheld NOTE A rechargeable Li Ion battery pack specifically for EXO Handhelds is available from YSI see sec
144. ration point Remove the central wiper from the EXO2 sonde before proceeding to the next step Next place the sensors in the correct amount of 300 ug L quinine sulfate standard in the calibration cup Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing verify that no air bubbles reside on the sensing face of the sensor If there are bubbles gently shake or move the sensor to dislodge When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu RFU l or 2 point Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water In the Calibrate menu select fDOM then select RFU Select either a 1 or 2 point calibration Enter 0 for first standard value and 100 RFU for second standard value Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Remove the central wiper from the EXO2 sonde before proceeding to the next step Next place the sensors in the correct amount of 300 ug L quinine sulfat
145. ration procedure is recommended if you are also using grab samples to post calibrate in vivo algae readings Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water In the Calibrate menu select BGA PE Chlor then select BGA RFU Select either a 1 or 2 point calibration When using Rhodamine WT enter 0 for the first standard value and 45 for the second standard value pg 93 Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde rg 94 Calibration DOM Review the basic calibration description in
146. re is splashing Keep away from food and drink Do not swallow If large amount is ingested and person is conscious induce vomiting First Aid See box on page 123 Turbidity Standard 6073 Ingredients Water Styrene divinyl Benzene copolymer beads The material is not volatile and has no known ill effects on skin eyes inhalation or ingestion Therefore no special precautions are required when using the standards However general precautions should be adopted as required with all materials to minimize unnecessary contact First Aid See box on page 123 Ultraviolet Light The DOM sensor radiates ultraviolet light UV light which can be harmful to the eyes even during brief periods of exposure Do not look into the light at the tip of the probe and wear protective eyewear when handling UV LEDs Lithium lon Battery Handling Failure to exercise care when handling this product and to comply with the following conditions and guidelines could result in product malfunction excessive heat fire property damage and ultimately injury e DO NOT alter puncture or impact battery or related components e DO NOT directly connect the terminals with metal objects e DO NOT expose the battery to extreme temperatures or direct extended exposure to sunlight e Always disconnect batteries when not in use and for long term storage e Store batteries in a non conductive and fireproof container e For best results store the battery at approximat
147. re to function No foreign objects can block the openings A Never expose the sonde or the cable to the atmosphere for more than a few minutes without an active desiccant or connector dummy plug in place Moisture or high humidity air entering the vent tubes can condense and block the tube affecting accuracy it could also cause damage to the transducer that is not covered by the warranty Special field cables are required for vented level measurements These cables have a vent tube running through the center and connect to the EXO sonde at the connector near the bail In the center of the sondes connector is a matching vent hole When attached the vented cable allows the sonde to vent through the water column and thus gain a more accurate depth measurement og 137 Vented Level Sonde 7 2 Installation When installing a vented level sonde users must ensure that the sonde never exceeds an operational depth of 10 meters Provisions for floods astronomical tides and severe storm events should be factored in Exposing the depth sensor to depths greater than 10 meters could result in damage to the pressure sensor that is not covered by the warranty Location of Depth Sensor For best measurement accuracy when installing a sonde the sonde s orientation and position must remain fixed When deploying the sonde vertically take care to ensure the sonde is redeployed in the same position Use a location pin or suspend the sonde using
148. rent points in a multi point cal 2 point Pour the correct amount of 0 NTU standard clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde into the water In the Calibrate menu select Turbidity then select Turbidity FNU Click 2 Point for the Calibration Points Enter 0 FNU for first standard value and 124 FNU for second standard value 0 must be calibrated first If the water to be evaluated is known to be low in turbidity an appropriate choice of standards might be 0 and 12 4 However for general purpose measurements an appropriate choice of standards is usually 0 and 124 If deploying with a copper anti fouling guard use this guard during calibration to calibrate for any offset input 0 5 or 1 instead of 0 The guard must be clean and free of sediment and debris Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point If the temperature of your field site is substantially different from the lab temperature allow the sensor to sample for 3 5 minutes at each calibration point before accepting it This step ensures the best possible temperature compensation when deployed Next place the sensors in the second calibration standard Cli
149. retaining screws back into their holes CAUTION Overtightening of the screws is likely to cause damage and require replacement of the battery pack pg 51 Rechargeable Li lon Battery Pack Overview The EXO Handheld HH can use a rechargeable lithium ion battery pack as a power source Battery life varies depending on GPS and Bluetooth wireless use Users can extend battery life by putting the HH in Sleep mode when convenient by pressing and holding the power button for less than three seconds AN WARNING Be sure to reference the supplied Safety Warnings and Precautions sheet included with the battery pack Failure to exercise care while using this product and to comply with the safety guidelines could result in product malfunction excessive heat fire damage to your products not covered under warranty property damage and or personal injury pep 52 1 Before the first charge Make a visual and physical inspection of the pack for any damaged or loose leads or connectors damaged packaging or other irregularities which may cause a short circuit and eventual fire Always charge the pack in an open area away from flammable materials liquids and surfaces Do not charge or handle batteries that are hot to the touch Remove battery cover panel The battery cover panel is located on the back of the HH To remove the panel unscrew counter clockwise the four screws with a flat or Phillips head screwdriver NOTE
150. ric Acid Nitrate Solutions 3885 3886 and 3887 Ingredients Water Potassium Nitrate Magnesium Sulfate Heptahydrate Gentamycin Sulfate Inhalation Avoid breathing vapors or mists Ensure adequate ventilation is available before handling Skin Wear lightweight protective clothing gloves and apron Eyes Wear safety glasses with side shields or face shield Contact lenses should not be worn when working with these solutions Ingestion May be harmful if swallowed Wear a mouth cover or face shield when there is splashing Keep away from food and drink First Aid See box at left Conductivity Solutions 3161 3163 3165 3167 3168 and 3169 Ingredients Water Potassium Chloride Inhalation Avoid breathing vapors or mists Inhalation of dust may cause irritation of respiratory tissues Ensure adequate ventilation is available before handling Exposure may cause irritation with repeated exposure Wear lightweight protective clothing gloves boots and apron al 142 Eyes Can cause irritation and potential eye damage with repeated exposure Wear safety glasses with side shields or face shield Ingestion May cause irritation of mouth throat and an upset stomach Wear a mouth cover or face shield when there is splashing Keep away from food and drink Do not swallow First Aid See box on page 123 pH 4 00 7 00 10 00 Buffer Solutions 3821 3822 and 3823 pH 4 Ingredients Water Potassium Hydrog
151. rs if possible before attaching wires 4 Connect flying lead cable to sonde Press in the male 6 pin connector then screw down the retaining collar Attach the cable s strain relief to the sonde s bail with a carabiner The cable s strain relief is designed to capture the sonde in case of failure of the connector 5 Prepare wires uN Always follow proper safety precautions when performing electrical work Properly strip the ends of the wire Remove 0 25 inches of insulation from each wire then twist the bared strands together All wires should be 18 24 AWG and are not included with the SOA 6 Insert wires into SOA Loosen the clamping screw with the supplied screwdriver insert the indicated wire into the terminal strip and tighten the clamping screw back down onto the exposed wire end Ensure that all strands are inserted to avoid short circuits Take care not to strip the slots in the heads of the screws e Connect DCP signal ground to SOA SDI ground terminal recommend black wire e Connect DCP SDI 12 data terminal for SOA SDI 12 terminal recommend violet wire e Connect DCP output ground terminal to SOA power ground terminal recommend black wire e Connect DCP 12 VDC output to SOA 9 16 VDC input terminal recommend red wire NOTE Due to the tight accessibility of many enclosures it may be more practical to complete Step 6 Wiring prior to Step 3 Mounting pg 47 Expansion Handheld RS 232
152. rush included in the sonde maintenance kit in clean water insert at top of channels and sweep the channels 15 20 times If deposits have formed on the electrodes use a mild solution of dish soap and water to brush the channels If necessary soak in white vinegar to aid cleaning Rinse the channels with clean water following the sweepings or soak 2 Short term storage When in regular field use the sensor should remain installed on the sonde in an environment of water saturated air Place approximately 0 5 in 1 cm of any water deionized distilled tap or environmental in the bottom of the calibration cup Insert the sonde and sensor into the cup and screw it on tightly to prevent evaporation More information in Short Term Sonde Storage section 6 1 3 Long term storage Store the sensors either dry or wet installed on the sonde or detached However before storage perform the recommended maintenance above to ensure the sensor is in good working order for the next deployment season If the sensor is submerged for storage ensure that the liquid is not corrosive pg 119 Dissolved Oxygen Sensor Storage EXO DO sensors require separate storage instructions from other optical sensors due to their sensing membranes This section will identify storage as long term or short term Long term denotes storage during times of long inactivity over winter end of monitoring season etc Short ter
153. rvals Burst Sampling Burst sampling allows the user to collect a set of data at each logging interval Activate Burst Sampling by clicking on Burst in the Logging Mode area of the Template Edit menu Then select a time duration between 1 and 300 seconds Data will be collected at a rate of 1 Hz during the specified duration This data set will allow users to perform advanced data analysis and post processing However note that this increased data in the logged file may more rapidly fill the internal memory of the data logger and affect battery life Adaptive Logging Adaptive or Event Logging allows the user to select one or two sensors as triggers for a higher resolution logging interval The user can set the trigger to activate above or below a pre determined threshold level for the given sensor Activate the Adaptive Logging feature by clicking the box next to Adaptive Logging Enter values for the logging interval and duration fields Then select a trigger sensor for Parameter 1 from the drop down list set the Mode to Above Below or Off and set the threshold value Repeat this process for Parameter 2 if desired However note that this increased data may affect battery life pg 70 KOR Software Sites Menu Used to manage sites this optional menu helps users create site detail that may be associated with files logged using the sonde or handheld KOR is a dynamic software platform subject to additional development and future improvements
154. s age accuracy is reduced ambient light rejection suffers and response times can be affected Maintain connectors as instructed in section 6 24 1 DO membrane maintenance Users should periodically inspect the optical surface at the tip of the sensor and wipe it clean with a non abrasive lint free cloth if necessary Never use organic solvents to clean an EXO DO sensor CP As much as possible prevent scratches and damage to the sapphire sensing window Avoid getting fingerprints on the window If necessary wash with warm water and dish soap and rinse with DI water 2 Sensor rehydration Users should always store DO sensors in a moist or wet environment in order to prevent sensor drift However should DO sensors be left in dry air for longer than eight hours they must be rehydrated To rehydrate soak the DO sensor cap in warm room temperature tap water for approximately 24 hours Following this soak calibrate the sensor and store it in a moist environment 3 Sensor cap replacement Due to restrictions inherent to all DO sensors DO sensor caps have a typical life of 12 months After this point users should replace the DO membrane cap To replace this cap follow the directions in the Sensor Cap Replacement section found on the next page pg 121 Dissolved Sensor Cap Oxygen Sensor Replacement Follow these instructions to replace the sensor cap on an EXO optical dissolved oxygen sensor once the prev
155. s compliant e Japan TELEC Radio Law compliant e Brazil Anatel certification compliant Reference the Declaration of Conformity in the next section for further details Bluetooth wireless technology and similar approvals and regulations can be country specific Check local laws and regulations to insure that the use of wireless products purchased from Xylem or its subsidiaries are in full compliance pg 145 EX Declarations of Conformity The undersigned hereby declares that the products listed below conform with all applicable requirements of FCC Part 15 for the U S and Industry Canada IC ICES 003 for Canada for intentional radiators Manufacturer Equipment name Model numbers Intentional Radiators Regulations VILLE YSI Incorporated a Xylem brand 1725 Brannum Lane Yellow Springs OH 45387 USA EXO Sondes EXO1 and EXO2 and EXO Handheld Systems 599501 xx 599511 xx 599502 xx 599512 xx 599150 EXO Sondes EXO1 and EXO2 contain the LMX Bluetooth module FCC ID ED9LMX9838 IC 1520A LMX9838 Nemko certified body ID CE 2302 EXO Handheld 599150 contains a Wi Fi Bluetooth module FCC ID U9R W2CBW003 IC 7089A W2CBW003 Nemko certified body ID CE 2302 e FCC 47 CFR Part 15 2011 Radio Frequency Devices e IC ICES 003 2004 Digital Apparatus Lisa M Abel Director Quality June 7 2012 pg 146 The undersigned hereby declares that the products listed below conform with all applicable Essential
156. se to the male pins on the sonde and the female connector on the USB SOA Then align the connector s six pins and jackets and press them firmly together so that no gap remains 2 Connect USB cable to SOA and PC Remove the protective cap from the USB end of the SOA and ensure that the connector is clean and dry Then insert the small end of the provided USB cable into the SOA connector and the large standard side into one of the PC s USB ports Attaching the adapter to the PC causes a new device to be recognized Windows automatically installs the drivers and creates a new port Each new adapter that is attached creates a new port Ports File Action View Help eo miOlamaeas KOR automatically scans ports for both USB adapters and Intel R WiFi Link 1000 BGN Bluetooth To view the USB adapter and its associated Pp EE Merec a r sat comm port go to the Control Panel on your computer D ortable Vevices a F Ports COM amp LPT click Device Manager then click Ports FI EXO USB Adapter COM19 id Standard Serial over Bluetooth link COM3 b a Procesors Next open KOR go to connections rescan and select the gt Security Devices i pill SM Driver j adapter from the list and click connect p a Sound video and game controllers m pg 43 Connect Sonde Bluetooth Before users can communicate wirelessly with their EXO sondes they must establish a Bluetooth link All EXO sondes are equipped with Blueto
157. section 5 1 Before calibrating be certain that the sensing window is clean cleaning instructions section 6 13 This procedure calibrates fDOM RFU or DOM QSU ppb If the user has both units selected then this procedure must be performed twice once for each unit to completely calibrate the parameter For 2 point calibrations the first standard must be clear water 0 ug L The second standard should be a 300 ug L quinine sulfate solution For detailed instructions for mixing this solution see section 5 11 AN Do not leave sensors in quinine sulfate solution for a long time A chemical reaction occurs with the copper on the sonde wiper assembly sonde bulkhead copper tape that degrades the solution and causes it to drift Also start with very clean sensors as the presence of chloride and halide ions from estuarine or seawater conductivity standards and Zobell solution can compromise QS fluorescence QSU 1 or 2 point Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water In the Calibrate menu select DOM then select QSU ppb Select either a 1 or 2 point calibration Enter 0 for first standard value and 300 ug L for second standard value Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calib
158. st 1 minute for temperature equilibration before proceeding Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Confirm that the Pending data value is close to the Setpoint value Click Proceed and wait for the software to prompt you to move the sensor to the next calibration standard solution Rinse the sensors in deionized water between changes of the calibration solutions Immerse the sensor end of the sonde in the pre chilled 1 mg L NH N calibration standard solution ensuring that the temperature is at least 10 C different than ambient Allow at least 1 minute for temperature equilibration before proceeding Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Confirm that the Pending data value is close to the Setpoint value Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu Rinse the sonde in tap or purified water Nitrate 3 point The calibration procedure for nitrate is identical to the procedure for ammonium except that the calibration standard solution values are in mg L NO N instead of NH4 N Chloride 3 point The calibration procedure for chloride is identical to the pro
159. sual Sum SANJO display differences are found between KOR s Desktop version aes peer O top and Handheld version bottom Graph This real time display will graph one or two parameters from the attached EXO device The parameters are chosen from the drop down menu above the plot area desktop KOR or with the tab and backspace keys handheld KOR Only parameters currently active in the attached device will be available for plotting It is important to note the scale when viewing real time data in Graph mode Due to the precise nature of EXO sensors very small micro changes are visible in the graph e Auto scaling enables KOR to best fit data into the available window even if the Y axis extremes vary by less than a unit e To manually scale the plot first deselect the Autoscale button s then click the upper most and bottom most numbers on either Y axis Not available on handheld KOR Capture Clear Advanced Data Buffer Plot Tools Clear Buffer As in the Dashboard the data buffer can manually be saved to a file using the Capture Data button Alternatively the buffer data can be cleared using the Clear Buffer button Once the buffer is cleared it cannot be recovered Settings The Settings menu defines the preferences for the RUN mode Plot line colors and width can be chosen as well as the default plot parameters While all parameters are available to set as default the connected device must have t
160. t move or rename other files This could damage KOR and affect system reliability Data files Templates binary data files data files and configuration settings are in the KOR EXO Data Files folder You can navigate to the KOR EXO folder to access template and data files if you want to copy them to another computer Program Files Data file names Data files are given unique names to ensure no duplication The file name structure is AAAAAAAA_YYMBBBBBB_MMDDYY_HHMAMSS bin Sample EXODT_12N768062_033012_182618 bin AAAAAAAA User defined file prefix up to 8 characters set in the deployment template or Run Settings menu YYMBBBBBB EXO sonde serial number YY represents the year the sonde was manufactured M corresponds to the month of manufacture and BBBBBB is a unique sequential number For live data capture files the serial number is a number assigned to the instance of Desktop KOR or the serial number of the Handheld MMDDYY MM is the month the data file was created DD is the day of the month and YY is the year HHMMSS UTC time stamp where HH is the hour the file was created in 24 hour format MM the minute and SS the second bin binary file extension To obtain a delimited file format or Excel format see section 4 6 oy 76 KOR Software SDI 12 The sonde can be connected to an SDI 12 bus using a DCP Signal Output Adapter SOA The SOA provides the necessary SDI 12 electrical interface and communicates to th
161. t accommodate the length of the canister The canister must be located within a few feet of the cable end e Remove the plug from the top fitting of the canister Remove the plug from the barbed fitting on the end of the cable Using the tubing provided in the kit connect the canister to the fitting on the end of the cable Remember to remove the remaining plug from the canister when ready to begin sampling When putting the sonde into service remove the plug to ensure that the sensor in the sonde is vented to the atmosphere pg 139 7 4 Calibration NOTE This calibration option is available only if your sonde is equipped with a vented level sensor For the calibration make certain that the vented level sensor is in air and not immersed in any solution Orient the sonde in the same position as it will be deployed Also never calibrate a vented level depth sensor with a non vented cable Depth Vented 0 10m Depth m Sernit 13N654321 Mounting Depth Offset Altitude 82 089 Latitude 36 456 V Check depth settings on Deployment _ Restore Calibration Defaults In the desktop KOR Calibrate menu select Port D Depth then select Depth m from the second menu In the Device Calibration menu you should see Depth Vented 0 10m as the device name In the handheld KOR Calibrate menu select Port D Dep V 0 10m then select Depth m from the next menu Click 1 Point for the Calibration
162. t to be disabled Press OK to a ag ale deployment and update firmware The Handheld will update the sonde and or sensors one by one and a progress screen will show when each is completed pg 115 6 12 Depth and Level Sensor Maintenance and Storage EXO depth and level sensors access the water through small holes ports located in the sonde body or bulkhead Although users cannot access them directly proper storage maintenance will help to ensure reliable operation Depth sensors can be stored dry in water saturated air or submerged in clean water However be sure that the water does not contain solutions that are corrosive This can cause damage to the sensor s strain gauge 1 Locate depth ports The two EXO1 depth ports are located in the yellow plastic section between the bulkhead tube labeled area and the blue plastic battery cover The EXO2 depth ports are located on the metal bulkhead face itself in the largest open area between ports 2 Clean depth ports Although users cannot directly access the depth level sensors they should periodically clean them with the syringe included in the EXO tool kit 599594 Fill the syringe with clean water and gently force water through one of the ports Ensure that water flows from the other hole Continue flushing the port until the water comes out clean A Do not insert objects in the EXO2 depth ports as this may cause damage to the transducer not covered under the warr
163. tact your local city office or your household waste disposal service DO NOT ship batteries to YSI Manufacturer We are committed to reducing the environmental footprint of our products While materials reduction is the ultimate goal we also make a concerted effort to responsibly deal with materials after a long productive life cycle Our recycling program ensures that old equipment is processed in an environmentally responsible way reducing the amount of materials going to landfills e Printed circuit boards are sent to facilities that process and reclaim as much material for recycling as possible e Plastics enter a material recycling process and are not incinerated or sent to landfills e Batteries are removed and sent to battery recyclers for dedicated metals pg 155 xylem Let s Solve Water wrw YSI Incorporated 1700 1725 Brannum Lane Yellow Springs Ohio 45387 USA Tel 800 765 4974 U S 1 937 767 7241 Fax 1 937 767 9353 environmental ysi com www EXOwater com Wissenschaftlich Technische Werkstatten GmbH D 82362 Weilheim Dr Karl Slevogt Strasse 1 Germany Tel 0881 183 0 info wtw de www EXOwater com EXO is a trademark of Xylem Inc or one of its subsidiaries Item 603789 Bluetooth is a trademark of Bluetooth SIG Inc Drawing A603789 Xenoy is a trademark of SABIC Plastics Revision E 2014 Xylem Inc Date August 2014
164. tc This set up is typical Logged data will be uniform e Set a custom start time Choose the start date and time which ee aes eee re eee can vary from minutes to days in the future then click the Apply pes mm button to prepare the sonde for deployment Setting a start time sora in the past causes the sonde to start logging immediately e Apply or Cancel Click the Apply button to apply the edits ee tisa to the settings Or click the Cancel button to update the BLUE sonde settings for SDI 12 but not actually start a log file Custom Time 00 00 00 PM MM DD YYYY CA Open a template Look ire CD Deployment Fies 3 EXO Deploymert dep This submenu opens saved template files on the computer LLA apret for configuration settings Deploy immediately by opening o default templates for 15 minute and one hour logging intervals which are stored in the KOR EXO Deployment Files folder located in the Program Files folder on the computer Template Edit Open and edit a template with a new name before deploying to the sonde Fie pame Fiesole deo de Basic To set up logging based on a specific time interval SDI 12 To send data to a DCP See sections 2 14 and 4 11 Advanced See below Save and deploy a template peee Save edited templates with these options e Save deploy start logging Starts logging at interval specified for deployment e Save deploy no logging Use with data logger and
165. te solution of Rhodamine WT R gt 0 9999 and this should ensure relative accuracy of field chlorophyll readings i e a chlorophyll reading of 100 units will represent twice the algal content of water with a chlorophyll reading of 50 units Also EXO chlorophyll readings show very low interference from turbidity allowing for more accurate determination of algal content during rainfall events which release both sediment and algae into the water The EXO chlorophyll sensor also exhibits very low interference from dissolved organics increasing data accuracy Blue green Algae The EXO BGA readings show excellent linearity on serial dilution of a surrogate solution of Rhodamine WT R gt 0 9999 and this should ensure relative accuracy of field BGA readings i e a BGA reading of 100 units will represent twice the algal content of water with a BGA reading of 50 units pa 28 Turbidity Sensor Overview Turbidity is the indirect measurement of the suspended solid concentration in water and is typically determined by shining a light beam into the sample solution and then measuring the light that is scattered off of the particles which are present The suspended solid concentration is an important water quality factor and is a fundamental measure of environmental change The source of the suspended solids varies in nature examples include silt clay sand algae organic matter but all particles will impact the light transmittance and result i
166. te with one sonde at a time Settings This submenu allows the user to control Bluetooth settings including PIN number and auto scanning Te EA Advanced e Oar BED This submenu allows the user to control settings for the 10 wt eat oat Signal Output Adapter SOA a COMA Berean soa mty KOR Software Help Menu This menu connects the user to documentation resources for using KOR software and the EXO products including videos pg 75 KOR Software Data Files and File Locations KOR software is installed onto your computer s default hard drive which is typically C on most Windows based PCs The KOR program is then placed into the program file directory On XP and Windows 7 32 bit systems this folder is simply called Program Files On 64 bit systems KOR is placed in the Program File x86 folder The two most common file paths are C Program Files KOR EXO XP and 32 bit Windows 7 C Program Files x86 KOR EXO 64 bit Windows 7 Data folders Data Files These are the binary data files from EXO which are only accessible via KOR We strongly recommend you maintain all binary data files in this folder and also create a back up copy Deployment Files Templates for deployments are stored here They may be moved to another computer to provide consistent deployments across your organization Site Files These contain the site details used by KOR future functionality not active yet Do not edi
167. ted level Reference section 7 for more detail on vented level The depth configuration must be chosen at time of ordering Once a sonde is shipped with a depth configuration it cannot be changed by the user Dissolved Oxygen Sensor Overview The principle of operation of the EXO optical dissolved oxygen sensor is based on the well documented concept that dissolved oxygen quenches both the intensity and the lifetime of the luminescence associated with a carefully chosen chemical dye The EXO DO sensor operates by shining a blue light of the proper wavelength on this luminescent dye which is immobilized in a matrix and formed into a disk The blue light causes the immobilized dye to luminesce and the lifetime of this dye luminescence is measured via a photodiode in the probe To increase the accuracy and stability of the technique the dye is also irradiated with red light during part of the measurement cycle to act as a reference in the determination of the Sensor Cap Sensor without Sensor Cap 59910001 599110 sensor cap luminescence lifetime When there is no oxygen present the lifetime of the signal is maximal as oxygen is introduced to the membrane surface of the sensor the lifetime becomes shorter Thus the lifetime of the luminescence is inversely proportional to the amount of oxygen present and the relationship between the oxygen pressure outside the sensor and the lifetime can be quantified by the Stern Volmer equation
168. term denotes storage during times the sonde will be used at a regular interval daily weekly biweekly etc Replace depleted sensor module as instructed in section 6 21 1 Sensor maintenance Ammonium or Nitrate sensor When deposits biofouling or other contamination appear on the membrane users should gently remove them with a fine jet of deionized water or rinsing in alcohol followed by soaking in the high standard calibration solution Gently dab dry with a lint free tissue Chloride sensor When deposits biofouling or other contamination appear on the membrane users should gently remove them by washing with alcohol and or gently polishing with fine emery paper in a circular motion to remove deposits or discoloration then thoroughly washing with deionized water to remove any debris A The ion selective membranes are very fragile Do not use coarse materials e g paper towels to clean the membranes as these could permanently damage the sensor The exception is fine emery paper for the chloride sensor noted above 2 Short term storage When in regular field use the sensor should remain installed on the sonde in an environment of water saturated air Place approximately 0 5 in 1 cm of any water deionized distilled tap or environmental in the bottom of the calibration cup Insert the sonde and sensor into the cup and screw it on tightly to prevent evaporation More information in Short Term Sonde Storage section
169. that does not occupy a sensor port The anode is available in two sizes for EXO1 sondes 599520 and for EXO2 sondes 599521 and attaches to the base of an antifouling sensor guard As with the other anode solution zincs are not required on early 2014 sonde models which feature a titanium bulkhead 1 Attach hardware to anode Equip the end of the socket head screw with the washer Then insert the screw into the anode base 2 Attach anode to sensor guard Attach the anode weight to the endcap of the antifouling guard Use an Allen or torque wrench to turn the socket head screw inside the anode weight Turn clockwise and tighten until snug torque to 24 30 inch pounds Do not over tighten A Only use the anode weight in combination with the EXO antifouling guard If you attach the anode weight to a polymer EXO sensor guard then the anode will lose all its protective qualities Order antifouling guards separately EXO1 599563 or EXO2 599564 pg 35 2 6 Install Remove e e Anti fouling Sleeves The Anti Fouling Protective Sleeve Kit 599663 contains plastic sonde and probe covers used for long term deployments The kit includes sleeves for the sonde individual sensors and the central wiper that are heat shrunk to be form fitting Throughout a deployment biofouling will collect on the sleeve rather than directly on the instrument significantly reducing cleaning and maintenance Review these instructions below
170. the posts on top of the sonde and remove the o ring from its groove and discard 3 Install new bail Install a new o ring in the groove at the base of the bail Then carefully spread the bail open and seat its sockets over the posts around the connector pg 109 Sonde Replace EXO2 Bail Sonde bails provide users with a handle for convenient transport and an attachment point for cable strain reliefs If an EXO2 bail breaks due to impact or standard wear and tear throughout the life of the sonde a user can easily replace it We also recommend attaching the cable s strain relief mechanism to the bail 1 Remove bail Use a small screwdriver to remove two screws on the sides of the bail Once screws are removed lift the bail off the sonde 2 Install new bail Place the new bail onto the sonde aligning holes for the screws With one side of the bail aligned push on the other side to snap it into place Use a small screwdriver to insert two screws on the sides of the bail Tighten until snug 6 7 Sonde Update Firmware amp KOR Software Users can check for and download new versions of KOR software through the EXOwater com website Click on Support and then the Software tab for the latest information and complete installation instructions Users can check and update sensor or sonde firmware through the KOR interface software Each device must be connected to the computer that is run
171. the rolling average entries are scanned for outlier data Although such data has already been determined to fall within the tolerances defined above the remaining worst offenders are removed from the rolling average calculation This outlier rejection allows for smoother continuous data results Calibration Stability During calibration the filtering is active as described plus an additional feature works to provide stability feedback to the user When the user attempts to calibrate a sensor the sudden changes in environment are perceived as impulses or plunge events and the filtering reacts accordingly The results immediately show the value of the solution and after a few moments the filter incrementally engages fully and supplies the smoothest data However as the sensor and the calibration solution work towards equilibrium the measurement may slowly drift The sensor will monitor the results from the filter and determine if the measurement is stable It watches the results and calculates a slope from each and every result to the next Once the slope settles and is consistently flat for approximately 30 seconds the sensor is considered stable KOR is then notified and calibration can continue Sensor Response Times Response times for EXO sensors are based on laboratory testing This testing though stringent cannot mimic the actual response times in the field due to the wide variety of use cases To characterize an EXO sensor s response ti
172. the sensor detects in a sample This parameter is typically used for diagnostic purposes only RFU stands for Relative Fluorescence Units and is used to set sensor output relative to a stable secondary standard such as Rhodamine WT dye This allows users to calibrate sensors identically for example calibrating all sensors in a network to read 100 RFU in a concentration of Rhodamine WT dye The sensors can then be deployed and generate data that is relative to all other sensors Once a sensor is retrieved it can be checked against that same standard to assess sensor performance drift or the potential effects of biofouling The ug L output generates an estimate of pigment concentration The relationship between ug L and sensor s RAW signal should be developed through following standard operating procedures of sampling the water body of interest collecting sensor data from sample and then extracting the pigment to establish a correlation The higher the temporal and spatial resolution of the sampling the more accurate this estimate will be Chlorophyll The EXO chlorophyll sensor operates on the in vivo fluorescence principle with no disruption of the cells required to obtain either spot readings or long term data The EXO sensor has an excellent detection limit as determined under laboratory conditions and this advantage should be realized in many field applications EXO chlorophyll readings show excellent linearity on serial dilution of a surroga
173. tings Open a Template and ifa connected sonde is logging a Stop Deployment button Read current sonde settings This menu scans the attached device and summarizes its Deployment time zone is current configuration including battery life sample count UTC 05 00 Eastern T US amp Canad ee ee and when the next sample will be taken The user can view the KOR EXO User Time Zone is UTC 01 00 Amsterdam Berlin Bern Rome Stockholm Vienna configuration edit the configuration or apply a saved template Do you wish to continue with deployment Time and Date When reading the current sonde settings Disable checkin Options gt gt User KOR communicates with the EXO sonde and performs a Gana F number of system checks including a date and time check If the sonde clock and the computer clock differ or if time zones differ then KOR notifies the user These prompts are informational only and can be disabled as noted on the screen Edit Edit the existing settings using the Edit button Deploy Redeploy the sonde by clicking the Deploy button with these log start time options e Start logging now For example a first sample logs at 11 32 31 and with a 15 minute logging interval the next sample will log at 11 47 31 e Start logging at next even interval For example a first sample logs at 00 00 00 and with a 15 minute logging interval the next sample will log at 00 15 00 then 00 30 00 00 45 00 e
174. tion date Send these commands to the DCP via an RS 232 hyperterminal window configured with the following settings Bits per second 9600 Flow control pel 78 None 4 12 Update Firmware and Software Sonde and Sensors Users can check and update sensor or sonde firmware through the KOR interface software This process may take up to 30 minutes depending on the number of sensors that will be updated NOTE For best power management update firmware while a device is connected via USB as this will provide power to the device However if you use Bluetooth we recommend installing fully charged batteries in the sonde oe Oa nb B amp O Update Firmware Updating pH SN 13A000314 firmware to version 2 0 0 Loading Application Section of Code Please Wait 1 Connect to sonde Each device must be connected to a computer running the Desktop version of KOR and the computer must have internet access 2 Open firmware submenu in KOR Navigate to the Options menu in KOR then to the Firmware submenu Immediately after clicking the Firmware submenu button KOR begins to search for connected sondes and sensors and loads the table with names serial numbers and current firmware versions 3 Update sonde and sensor network Click the update network button KOR then updates the device s firmware which could take several minutes If you experience problems with automatically d
175. tion Settings menu w _ K 0 1 0 561 27 PM 733 8244 Bar mmHg 3 Rescan sonde If a wired connection is not found and to manually nottom 10N700445 establish a connection to a sonde via Bluetooth navigate to the Connections menu in KOR software on the Handheld Select Rescan KOR will rescan and detect Bluetooth enabled sondes Select the sonde from the list and then click the Connect button Detected Device s If no sonde is detected click Refresh to scan again It may take 2 3 scans to discover the sonde pg 56 Spot Sampling View Live Data When connecting through the Handheld KOR software attempts to automatically connect to an available sonde and start displaying current data in a live view To manually access the live view from either the Handheld or Desktop go to the Run menu and choose Dashboard Users can set view preference in the live view menu to display numeric live data default or a graph view where a maximum of two parameters can simultaneously be plotted on the screen For overview of KOR menus see section 4 for overview of Handheld keypad functions see section 1 3 Garan m o gt m Data dashboard and graph 22 682 37 6 all o Users can toggle between numeric Dashboard and Live aig 734 4 0 21 Graph views using the soft key on the left The Capture Cond en ean O TEEPEE Data soft key may be used to log information displayed on 87 4 78 08 0 0 SPC usjem 1 Tub FNU 4 Cable Pwr V 0
176. tion 3 2 3 Reinstall battery cover panel Ensure that the rubber battery cover gasket is seated properly then replace the cover onto the back of the HH Tighten the four retaining screws back into their holes N CAUTION Overtightening of the screws is likely to cause damage and require replacement of the battery pack pg 113 Car Handheld Update Firmware and KOR Software To update the instrument firmware and KOR software on the EXO Handheld use the Desktop version of KOR on a computer with internet access KOR Desktop will go online and pull updated files for the Handheld which are then transferred to the Handheld via cable Bluetooth communication is not recommended for this process 1 Connect handheld to computer Plug the small end of the USB cable into the port on the top side of the EXO Handheld Plug the other end of the USB cable into a port on your computer Power on the Handheld and allow a minute for Windows to recognize the Handheld as a removable drive before the Handheld shows up in KOR software 2 Update handheld When the Handheld is connected to the PC go to the Options Firmware menu in KOR Desktop software Select the Update Handheld button from the bottom right corner of the menu Follow the prompts for completing the update process and rebooting the Handheld Copying Handheld Firmware Files Attention Do not disconnect Handheld or remove power during this process Copying Insta
177. tion point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde rg 92 Blue green Algae Phycoerythrin This procedure calibrates BGA RFU or BGA ug L If the user has both units selected then this procedure must be performed twice once for each unit to completely calibrate the parameter For the 2 point calibration one of the standards must be clear water 0 ug L and this standard must be calibrated first The other standard should be in the range of the suspected BGA PE content at the environmental site Two general types of standards can be used a phytoplankton suspensions of known BGA PE content and b dye solutions whose fluorescence can be correlated to that of BGA PE The user is responsible for determining the BGA PE content of algal suspensions by using standard cell counting techniques For option b we recommend using a 25 ug L Rhodamine WT dye solution for detailed instructions see section 5 11 and the solution is used in the calibration steps below ug L l or 2 point This procedure will zero your fluorescence sensor and use the default sensitivity for calculation of phycoerythrin containing BGA in ug L allowing quick and easy fluorescence measurements that are only semi quantitative with regard to BGA PE However the readings
178. tivate their sondes users should keep a magnet with them when setting up and deploying sondes For more information on sonde states and LEDs see section 2 7 1 Awaken sonde with magnet Users can make their sonde go to the Awake state by holding a magnet at the magnetic activation area on the sonde s bulkhead identified by the illustrated magnet symbol on the label Simply hold the magnet within one 1 cm of the symbol until the LEDs activate EXO Handhelds and sensor tools contain embedded magnets identified by the same symbol NOTE A new magnetic tool is available Item 599469 EXO Sensor Tool Kit 2 Awaken sonde without magnet Users can also make their sonde go to the Awake state using any of the following methods e Cycling power to the sonde uninstalling installing batteries e Communicating via the topside port e Inserting a sensor In addition to these manual methods the sonde also automatically awakens for scheduled unattended logging programmed in KOR 3 Activate sonde s Bluetooth Users activate Bluetooth by holding a magnet at the magnetic activation area in the same way as described in Step 1 In addition to magnetic activation users can also activate Bluetooth by e Cycling power to the sonde uninstalling installing batteries e Enabling Bluetooth via a connection at the topside port in KOR pg 39 Attach Sonde to Handheld Field Cable All EXO cables have 6 pin and wet mateable conne
179. tive Site Ohio River Sitel 0 44 49 Chattahooche River D 7 4 Select File to View 24 Total Files NAME KOR_Walkthrough_11N502313_121412_163955 0 68 SIZE kB EXOHH_13C101341_061813_095304 bin 0 64 abc_13C101341_062413_115659 bin 236 73 D FP 22 682 57 6 0 21 Temp C 1 pH mV 2 BGA PC RFU 3 0 21 BGA PC ugh 3 83 6 734 4 Cond uskm 1 Baro mmHg 8 Teal header notification confirms data capture 734 2 Baro mmHg D _EXOHH Graph Type Tine Series _ S Defaut Ven E Dashboard Defaut Param Af Teme C S Defaut Param E Temp lt _ lt show Sonde Date f Disabled Sample interval 0 5 sec pg 58 1 Logging data Upon navigating to the Capture Data menu for the first time and each time you reconnect to a sonde you will need to set a logging file or start logging with a new file options 3 or 4 Logging to a new file will automatically generate and set a Current Logging File to which future data points will be appended 2 Appending data Ra Once a logging file is set you can append future data points to it Use the keypad to select options 1 or 2 to add data points to the Current Logging File To append data to a different location press 5 on the keypad This will bring up a list of available files currently saved on the handheld that can be set instead NOTE You can press 1 or 2 while on the dashboard to quickly append data to an existing file a
180. to assure high performance Calibration procedures follow the same basic steps with slight variations for particular parameters Conduct calibrations in a temperature controlled lab Calibration set up For accurate results thoroughly rinse the EXO calibration cup with water and then rinse with a small amount of the calibration standard for the sensor you are going to calibrate Two to three rinses are recommended Discard the rinse standard then refill the calibration cup with fresh calibration standard Fill the cup to approximately the first line with a full sensor payload or the second line with small sensor payload Volumes will vary just make certain that the sensor is submerged Be careful to avoid cross contamination with other standards Fill to line 2 Fill to line 1 P f Begin with a clean dry probe installed on the EXO sonde Install io the clean calibration guard over the probe s and then immerse the probe s in the standard and tighten the calibration cup onto the EXO sonde We recommend using one sonde guard for calibration procedures only and another sonde guard for field deployments This ensures a greater degree of cleanliness and emails accuracy for the guard used in the calibration procedure SpCond pS cm eee sows S 12A101235 Basic calibration in KOR software Matching unii Go the Calibrate menu in KOR software This menu s appearance will vary depending on the sensors installed in the sonde Select
181. tter than the competition while providing the advantages of easy integration into a multiparameter package and automatic mechanical cleaning when used in monitoring studies with an EXO2 sonde p22 Ammonium Nitrate amp Chloride Sensors Overview JN nace ite nitrate and chloride ion selective electrodes ISEs should be used in freshwater applications only at depths of less than 55 feet 17 meters and less than 25 psi The ammonium and nitrate sensors use a silver silver chloride wire electrode in a custom filling solution The internal solution is separated from the sample medium by a polymer membrane which selectively interacts with ammonium or nitrate ions When the sensor is immersed in water a potential is established across the membrane that depends on the relative amounts of ions in the sample and the internal solution This potential is read relative to the Ag AgCl reference electrode 599709 599710 599711 599743 01 599744 01 599745 01 modules Specifications Ammonium NH Temperature Operating Storage o 30 C 0 lt 55 ft 0 to lt 17 m 10 of reading or 2 mg LN whichever is greater Sensor Type lon selective electrode WORE nee e ee pew EEE S PUREE TTL LC SETS TCC TT ELELETe Tee Cee ee eT Tee Conductivity lt 1500 pS cm 2 mg LN millivolts Temperature Operating 10 of reading or 2 img L N whichever is greater Sensor Type lon selective electrode dotoplalela ale ain
182. uce problems associated with traditional underwater connectors However these connectors must be properly maintained to reap the full benefit of this design Following these instructions will minimize most potential issues Never stick any foreign object into a female connector Use only Krytox grease to lubricate the mating surfaces of the connectors 1 Female 6 pin connectors These connectors are located on field cables EXO2 accessory connector and EXO Handheld Periodically inspect the connectors for signs of contamination If you detect debris remove it with a gentle blast of compressed air Prior to initial installation or when dry apply a light coat of Krytox grease to the flat rubber mating surface on top of the connector When not in use always install the connector s plug 2 Male 6 pin connectors These connectors are located on field cables and topside sonde connectors Periodically inspect the connectors for signs of contamination If you detect debris carefully remove it Prior to initial installation or when dry apply a light coat of Krytox grease to the rubber mating surfaces of the connector including the rubber portions of the pins When not in use always install the connector s plug 3 Sensor connectors 4 pin These connectors are located on sonde bulkheads sockets and sensors Periodically inspect the female portions of these hermaphroditic connectors and the entire socket for contamination and rem
183. uld periodically inspect the optical surface at the tip of the sensor and wipe it clean with a non abrasive lint free cloth if necessary As much as possible gt prevent scratches and damage to the sensing window hi 2 Long and short term storage Turbidity Total Algae and DOM require minimal precautions Users can either remove the sensors or leave them installed in the sonde for long and short term storage If left installed on the sonde follow guidelines for sonde storage If users remove them from the sonde the sensors may be stored in dry air in their shipping cap to protect against physical damage Z Do not store any sensor in quinine sulfate solution al 118 6 14 Conductivity Temp Sensor Maintenance and Storage EXO conductivity and temperature CT sensors require little maintenance or special attention for storage As much as possible prevent impact to the sensor s exposed thermistor This section will identify storage as long term or short term Long term denotes storage during times of long inactivity over wintering end of monitoring season etc Short term denotes storage during times the sonde will be used at a regular interval daily weekly biweekly etc Maintain connectors as instructed in section 6 24 1 Clean electrode channels The only parts of the CT sensor that require special maintenance are the channels leading to the internal electrodes Dip the sensor s cleaning b
184. ummy plugs Cable Storage Users should leave the cable installed on the sonde to protect the connectors If necessary users may remove it from the sonde but extra care should be taken to protect the connectors For vented cables ensure the storage cap is affixed to the desiccant inlet Store vented cables in a bag containing desiccant NOTE Minimum bend radius for coiling cable is 8 inches pg 141 Health and Safety Chemicals NOTE For additional health safety and disposal information about reagents download the MSDS documents for the chemical in question from the EXO manufacturers websites www ysi com or www wtw de First Aid for all solutions Inhalation Skin Contact Eye Contact Ingestion Skin Move to fresh air If breathing is difficult give oxygen If i symptoms persist seek medical attention contaminated clothing and wash Wash exposed area with soap and water for at least 15 minutes If irritation persists seek medical attention Rinse eyes immediately with large amounts of water also under eyelids for at least 15 minutes It irritation persists seek medical attention Wash out mouth with water and then drink plenty of water If symptoms persist seek medical attention Ammonium Solutions 3841 3842 and 3843 Ingredients Water Ammonium Chloride Lithium Acetate Dihydrate Sodium Azide Hydrochlo
185. und in Standard Methods for the Examination of Water and Wastewater ed 1989 The use of the Practical Salinity Scale results in values that are unitless since the measurements are carried out in reference to the conductivity of standard seawater at 15 C rg 16 LEYA Depth and Level Sensor Overview EXO measures depth of water with a non vented strain gauge See section 7 if your sonde is equipped with vented level A differential strain gauge transducer measures pressure with one side of the transducer exposed to the water and the other side exposed to a vacuum We calculate depth from the pressure exerted by the water column minus atmospheric pressure Factors influencing depth measurement include barometric pressure water density and temperature Calibration in the atmosphere zeros the sensor with respect to the local barometric pressure A change in barometric pressure will result in a zero shift unless the transducer is recalibrated to the new pressure EXO2 Depth Intake EXO 1 Depth Intake Depth Sensor Location relative to other water quality sensors see EXO sonde label Depth Sensor Location 27 2 cm EXO1 13 9 cm EXO2 to WO Sensors EXO sondes have intake openings to allow water to act on the strain gauge The EXO1 intake is located in the yellow section between the battery compartment and label of the sonde The EXO2 intake openings are two small holes on the face of the sonde bulkhead continued
186. urn to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde RFU l or 2 point RFU is a percent full scale output it outputs relative fluorescence from 0 100 This calibration procedure is recommended if you are also using grab samples to post calibrate in vivo chlorophyll readings pg 90 The sonde will report relative values of fluorescence in the sample being measured These values can be converted into actual chlorophyll concentrations in ug L by using a post calibration procedure after the chlorophyll content of grab samples taken during a deployment has been analyzed in a laboratory This determination can involve conducting the extractive analysis procedure described for chlorophyll in Methods for the Examination of Water and Wastewater or by carrying out an in situ measurement of chlorophyll using a commercial benchtop fluorometer Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water In the Calibrate menu select BGA PC Chlor then select Chl RFU Select either a 1 or 2 point calibration Enter 0 for first standard value and 16 4 for second standard value Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds c
187. ution Next place the sensors in the medium containing a known oxygen pressure or concentration and wait at least 10 minutes for temperature equilibration Click Proceed in the pop up window Then enter either the barometer reading in mm Hg for ODO or the actual concentration of oxygen which was probably determined from a Winkler titration for ODO mg L Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Carrying out DO mg L calibrations at values outside the range of 10 of air saturation is likely to compromise the accuracy specification of the EXO sensor For highest accuracy calibrate in saturation Rinse the sonde and sensor s in tap or purified water and dry pg 84 Calibration Depth and Level NOTE This calibration option is available only if your sonde is equipped with an integral depth sensor or a vented level sensor For the calibration make certain that the depth sensor or vented level sensor is in air and not immersed in any solution Also review the basic calibration description in section 5 1 In the Calibrate menu select Port D Depth then select Depth or Level from the second menu Cl
188. vigation Both versions of KOR Desktop KOR for computers and KOR for the EXO handheld have the same basic menu structure The main menu items are Run This menu is used to display live data from the EXO sonde either in numeric or graphical display Calibrate This menu is used to calibrate the sensors installed in the EXO sonde after the sonde is connected Deploy Go to Deploy to set up the EXO sonde for logging deployments Settings such as logging interval and SDI 12 configurations are in this menu as well as starting and stopping autonomous logging on the sonde Sites Used to manage sites this optional menu helps users create site detail that may be associated with files logged in the sonde NOTE Only available on desktop KOR Data This menu enables file transfers from the EXO sonde or handheld and can also be used without a sonde to view and export data files that were previously transferred to the desktop or handheld Options Application preferences sonde settings and software and firmware updates can be accessed through this menu The user can change display units here Connections This menu allows the user to connect to other sondes and devices identify which probes are installed in which ports and update Bluetooth settings Help This page links to support resources for using KOR Status icons The icons in the top right of the KOR window are information only displays The white box shows the conne
189. voiding the need to navigate to the Capture Data menu for each reading Continuous sampling Selecting options 2 or 4 on the Capture Data menu will cause the sonde to begin recording data continuously at the sampling interval set in the Run Settings Run gt Run Settings To stop logging data press Stop Capture 4 File nomenclature With the addition of sites options for file naming have been added in the Run Settings menu of the Handheld Run gt Run Settings EXO data files are generated with the following naming structure by default FilePrefix_HandheldSerialNumber_MonthDateYear_ HourMinuteSecond bin You can modify the File Prefix in the Run Settings menu using the Handheld s alpha numeric keypad Additionally you can also include sites within file names by using the directional keys to select Site Name under Capture File Name This naming structure is FilePrefix_Site Name_MonthDateYear_ HourMinuteSecond bin EX3 Managing Sites with Handheld The desktop software and handheld firmware allows you to create delete sites as well as tag data files with site names 6 08 Chattahooche River D F amp O pt O 1 Log 1 point to current file Start logging with current file Log 1 point to new file Start logging with new file Change current file Change active site Current Logging File lt Not Set gt Active Site Ohio River Sitel Select Site From Handheld NAME tim sit 1 Creat
190. ware must first be installed on a computer before transferring files from Handheld to PC koe 1 4 7 1070 Select Files to Delete Look ie C Deployment Fies 3 B20 Deployment dep Fle pame My Network Fies of ype Connect handheld to computer Power on the Handheld and allow up to three minutes for Windows to recognize the Handheld as a removable drive before the Handheld shows up in KOR software Plug the small end of the USB cable into the port on the top side of the EXO Handheld Plug the other end of the USB cable into a port on your computer Transfer files When the Handheld is connected to the PC go to the Options Sync with Handheld menu in KOR Desktop software Select Sync All and KOR will search all folders and synchronize the files automatically Or users can select each folder separately and synchronize selected files manually By checking the delete files box data and calibration files will be removed from the Handheld after syncing Push Latest Firmware Package button will transfer the most recent sensor and sonde firmware from the PC to the Handheld for updating sondes in the field with the Handheld Delete files Directory Maintenance Users can browse folders and select files to delete from the Handheld Deleting files is optional EST Handheld Options Upon startup of the Handheld the GPS function automatically initiates a fix of the location This may take some time During this pro
191. will reflect changes in BGA PE from site to site or over time at a single site Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water In the Calibrate menu select BGA PE Chlor then select BGA ug L Select either a 1 or 2 point calibration When using Rhodamine WT enter 0 for the first standard value and 126 for the second standard value Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde in tap or purified water and dry the sonde RFU l or 2 point RFU is a percent full scale output it outputs relative fluorescence from 0 100 This calib
192. wise to remove Always use one guard for deployment storage and the other for calibration only AN Take care not to let the guard damage unguarded pH or pH ORP sensors when installing and removing 2 Install remove calibration cup Before installation loosen but do not remove the cup s clamping ring Then with the sonde guard already installed slide the cal cup over the guard until the bottom of the guard rests against the bottom of the cal cup Tighten the ring until snug To remove the cal cup loosen the ring by 1 4 turn and pull the guard free from the cup pg 32 2 3 Install Remove Sensors EXO sensors have identical connectors and identify themselves via onboard firmware therefore users can install any probe into any universal sonde port The exception is the wiper for the EXO2 sonde which must be installed in the central Port 7 Individual ports are physically identified by an engraved number on the sonde bulkhead Although the probes are wet mateable users should clean lubricate and dry the sonde and sensors connectors prior to installation or service when possible 1 Remove probe or port plug Remove the calibration cup and sensor guard from the sonde Place the sonde on a clean flat surface and prevent it from rolling If removing a sensor or port plug use the probe tool in the locking nut and rotate counterclockwise to loosen Pull the probe straight out of the port and place on a clean surface Remov
193. y pack is an available option as described in the next section Users can extend battery life by putting the HH in Sleep mode when convenient by pressing and holding the power button for less than three seconds 1 5V rechargeable high capacity NiMH batteries can also be used Battery life varies depending on GPS power to sonde and Bluetooth wireless use 1 Remove battery cover panel The battery cover panel is located on the back of the HH To remove the panel unscrew counter clockwise the four screws with a flat or Phillips head screwdriver NOTE The retaining screws are captured into this panel and are not independently removable If replacement is necessary replace the entire assembly NO Insert replace batteries A Do not use 3 6V Li batteries in the handheld Damage to the circuit board is not covered under warranty Remove the old batteries and dispose of them according to local ordinances and regulations Install the new batteries between the battery clips with their polarity oriented as shown on the bottom of the battery compartment If you use your own rechargeable batteries they cannot be charged inside the handheld they should be charged outside the handheld If using Ni MH batteries we recommend high discharge type with 5 000 mAhr rating 3 Reinstall battery cover panel Ensure that the rubber battery cover gasket is seated properly then replace the cover onto the back of the HH Tighten the four

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