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User`s Manual

1. Buttons 1 5 Program Keys 6 Speed RCF Toggle 7 Time Radius Toggle 8 Acceleration rate set 9 Deceleration rate set 10 Stop Run 11 Pulse Run 12 Start Run 13 Temperature set CLAOR FL40R Display Screens 15 Upper Screen speed RCF 16 Middle Screen time raduis 17 Lower Screen accel decel rates temp R mod CLAOR FLAOR Thermo Electron Corporation LED Lights la Program 1 Indicator Za Program 2 Indicator Ja Program 3 Indicator 4a Program 4 Indicator 5a Program 5 Indicator 8a Acceleration Indicator 9a Deceleration Indicator 13 Imbalance Indicator 14 Lid Opening allowed Indicator 18 Speed Indicator 19 RCF Indicator 20 Time Indicator 21 Radius Input Indicator CL40 FL40 4 1 Chapter 4 Instructions for Use Display Screens Screen Description Left screen 15 This screen displays the set speed rpm or RCF x g for the given program when the centrifuge is at rest depending on which parameter is selected During a run this screen displays actual speed or RCF Middle screen 16 This screen displays either the set run time or the centrifugal radius when the centrifuge is at rest depending on which parameter is selected During a run this display shows only time remaining timed runs or time elapsed during deceleration hold runs and pulse runs Right screen 17 This screen displays either the acceleration or the deceleration profile selected for the give
2. CL40 FL40 vii viii CL40 FL40 Chapter 1 Chapter 2 Chapter 3 Chapter 4 Contents Use and FONCTION messi sama asmaya on 1 1 User Manta EN 1 1 Warming ya bol A YY AN RA ER 1 1 e E 1 1 Refrigeration and Heating Systemes dan 1 2 IR 1 3 Safety Interlock st slds 1 3 Imbalance Detection Zeta aie n E R 1 3 Relative Centrifugal Force pic 1 4 Specifications aiii 2 1 Dimensions and Wei tinca mesa enmli milinin 2 1 Centrifugation ea a ces ebd sdb 2 1 Electrical Specifications pd 2 2 Installatign acia 3 1 Environmental Condo ias 3 1 Unpaid 3 1 CO RK A A A NTETE NEEE ENER 3 2 E 3 2 Lid Opening and Rotor Checking sia 3 3 Manual Lid unlocking Procedure scada RR 3 3 Performance and Accessories iaa dida 3 5 M4 Swing out Rotor Cat N 11175338 pajas 3 5 Bi containeiment ACCESSOrIES iia 3 6 QG2 5 Swing out Rotor Cat N 11175982 ns 3 8 ANPE ROTORS a 3 8 a le ee 3 10 Instructions tor USE initial 4 1 Controls and Indicators E 4 1 IB pos MR 4 2 Control Panel Funcion alli 4 3 Control Panel Indicators eiii dia 4 4 Description of Certain Events with Respect to the Control System 4 5 Preparing the First Run of the Da male izlel kaide ez 4 7 Sample Loading ii 4 7 Thermo Electron Corporation Thermo Electron Corporation Chapter 5 Chapter 6 Chapter 7 Contents Hazards Precautions and Limitations of Use 5 1 TEC 101022020 EE 5 1 A Ns eese ea le illede eli 5 1 S
3. S 0 0 0 0 Carbazole 0 S 0 0 0 S 0 0 U U 0 0 0 U Carbon Sulphide S U MO 0 St M S U 0 0 0U St 0 0 0 St U S SM S 0 0 0 0 0 0 MoO 0 0 0 X Carbon Tetrachloride 0 0 0 0 0 0 0 0 0 U Cedarwood Essence Chlorine dry 0 0 0 Sd St Mt U 000000 0 Mt Sd St Chlorine moist o O O INO O o QO O ww O O mm zz ojojo gt jojo lc o o O o O O cm O O JOIN O O 2 S e VE Oo JEE L AIM DIDIS r jm lc AIMIDIDIS nN In m 5 Lmom5 5IooIIIz i AI M PDIPIS m wm Ja oo o 2 o o o o O O O O O O ln jo o IO c O C c C Ie o L WEE OD oo 3m JOJO D OVO 3 3 O LE 3 C e 615 EECH 3 D gt SOS lt S 5 gt gt D c SEA ols s esses SS o st le c eim 0 160 E 8 BEES ol2lalealal el el e pue eo EE elgs lelelelele e lt lolelelelelele Olajolo jo jo lo jo 0 0 S 0 0 S U MO 0 S U S 0 0 0 0 0 0 0 0 U St S 0M S SM S S Cinnamon Essence Citric Acid 1096 Copper Nitrate S S S S S 0 0008 085 5000 0 U 0 0 0 0 6 5 CL40 FL40 Thermo Electron Corporation Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO U 0 0 0 0 0 0 0 8 S 0 0 0 0 Chemical S 0 0 0 0 0 0 0 St Copper Sulphate Croesol S 0 0 SMS 0 U SU S Sd
4. Formalin 37 formaldehyde in water in contact for 10 minutes has a similar effect to chlorine bleaches Rotors should be thoroughly rinsed under running water for 5 minutes to remove all traces of formalin then dried completely Formaldehyde is toxic A Gluteraldehyde 2 sold under many brand names such as Cidex and Glutarex requires total immersion for 10 minutes to ensure sterility Thorough rinsing and drying is essential to protect users Gluteraldehyde builds up to a toxic level in the fatty tissues of the body Phenols are very corrosive and should never be used A We recommend that all radioactive contamination be referred to your Radioactivity Safety Officer Rotors may be decontaminated by a mixture of equal volumes of e Distilled water e SDS diluted to 10 e Ethanol diluted to 70 The rotor should then be rinsed with ethanol followed by distilled water and then dried completely Thermo Electron Corporation Chapter 6 Service and Maintenance Thermo makes no claims as to the effectiveness of proprietary brands of decontaminating solutions Power Supply Circuit If the main circuit breaker of the centrifuge cuts off the power to the Bre aker instrument do not attempt to switch it on before a Thermo Service representative has checked over the unit Rotor Removal To remove the rotor from the motor shaft e Using the wrench supplied remove the rotor lock nut by turning the wrench clockwise e Using th
5. S 0 0 0 0 Diphenyloxide S S 0 0 0 0 0 S S O 0 0 0 0 00 0 8 45 Dipropylene Glycol Distilled Water Ethanol 50 S S S S S 0S SS SS S S S S S S S S S S S S U SM S S S M S miololjolojololjn lo lo coc o o o o o v o co co O O sO O O o o coc o o o o o MNI O w wlololololololololo molina qn o ns gl l ls r S S co c l ln l AS e zm polos JD gt w S gt AIS SIF AlA AI Y S E 3 3 5 5 gt jo lo vlololololololololo wlnlolololololnlolo e 0066 1061010 gt gt liojojoljojo lio lo 2 2 o o o lo lo ce o o o o o wev o o a gt O O O W toi O D Elslo po D o S lt 2 2 3 2 8 2122 oia ole 2 EL EX l ol gt slalalslslolololw D E NG 9 o0o05 g2gm E seem eee s es o E D Si zl zl zl zl S ES Am E E O Ale 22 dE E EE 4 rer kel kel ELI ELI ELI II ELI II II HI II ES S SMO 000 St S S 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Ethyl Lactate M S S MO 0 0 0 0000 Ethyl Malonate Fatty Acids S 0 0 0 0 0 0 0 Sd Sd Sd Sd S 00 0 0 0 S 0 S ODU S S S 0 OM S S S S M0000000 S U Ferric Chloride Ferric Nitrate SM 0 0 0 0 0 0 St 0 S S Sd St S SM S S 0 0 0 0 0 0 S S 0 S 0 0 00 0 0 0 M 0 0 0 0 00 0 S S 0 0 0 0 U UMMU Ferric Sulphate Ficoll Paque Fluorine 0 0
6. When the button 9 is pressed the lower display 17 shows the number corresponding to the deceleration profile currently registered 1 to 5 5 being the fastest To change the value press the corresponding up down arrows Set temperature R only To increase decrease the temperature set values press on the corresponding up down arrows To pre program the centrifuge There are five available memory locations for pre programmed protocols Each corresponds to one of the five memory buttons 1 5 To enter save a program into one of the locations press the memory button corresponding to the desired program position The corresponding LED 1a 5a will be illuminated While the LED is illu minated enter the desired parameter values These values are automatically saved in memory To lock a program To lock a program press and hold the corresponding button for several seconds until the correspon ding LED begins to flash Release the button and the program is locked Parameters of the program cannot be changed To unlock a program Thermo Electron Corporation To unlock a program when a program is locked the associated LED flashes press and hold the corresponding button for several seconds until the associated LED stops flashing Release the but ton and the program is unlocked CL40 FL40 4 3 Chapter 4 Instructions for Use Control Panel Indicators Indicator Description When the lid interlock in
7. yo olli o o ojojojojo jm olololo lol co o o jojo gt pojojojojo gt jojojojojm Ss jojojo jo jo gt pojojojojo g o 5551lm E 2 D m SIE E o 2 Jel lt 2 ja o aks SEE o GH EE ed HE 2 212 a olOlala 2 uzccmssss lt S9 Elololo E Ap DB BZ A Az S 0 M U 0 S S S S 0 S S S S S S S S U S 0 S S SS sS S U U 0 S S M 0 Potassium Chlorate 0 S S US S u 0 0 00 0 0 0 UM SM LU Potassium Chloride S S S 0 0 S S Potassium Hydroxide 5 M S 0 S S UU 0 0 0 S S 0 0 0 0 0 0 0 8 U M Potassium Hydroxide conc Potassium Nitrate S 0 0 0 0 0 0 S D 0 S 0 0 S 0 0 0 0 0 0 0 S Potassium Permanganate Propane Gas U M 0 0 000 S 0 0 0 0 0 0 0 U St 0 0 0 0 0 0 0 0 Sd U M Sd S 0 0 0 0 0 0 Propionic Acid S 0 0 0 0 0 0 S S 0 0 0 0 0 ss 0 0 0 0 0 St S 0 0 00 0 0 0 Propyl Alcohol S 0 0 00 00 0 S Sd s 0 0 0 00 0 0 0 8 St Propylene Glycol S Propylene Oxide Pyridine U 0 0 0 0 000 MU 5S M 0 0 0 0 0 0 000 0 0 00 0 8 Sd s U 0 0 0 0 0 S Resorcinol Sat d Sol Thermo Electron Corporation CL40 FL40 6 8 Chapter 6 Service and Maintenance Material AL BN CAB CN DL KY NO NY PA P
8. 20 rpm display Thermo Electron Corporation CL40 FL40 2 1 Chapter 2 Specifications CL40 FL40 CL40R FL40R RCF set display Range 20 9900 20 10400 Step 10 x g at less than 1000 x g 10 x g at less than 1000 x g 100 x g at above 1000 x g 100 x g at above 1000 x g Accuracy 20 x g display 20 x g display Timer set display 30 s 99 min continuous 30 s 99 min continuous Temperature set display 9 C to 40 C 9 C to 60 C Acceleration rates 5 5 Braking rates 5 5 Drive system direct direct Brushless induction motor yes yes Electrical specifications CL40 FL40 CL40R FL40R Nominal operating voltage 230V 230 V 10 230 V 10 120V 120 V 10 120 V 10 Power requirements maximum 1200 W 1500 W Heat output maximum 3750 BTU h 4435 BTU h 2 2 CL40 FL40 Thermo Electron Corporation Environmental Conditions Unpacking Thermo Electron Corporation Chapter 3 Installation General conditions accepted for centrifuge transport and storage are e Ambient temperature 20 C to 50 C e Relative humidity up to 90 General conditions accepted for operating the centrifuge safely are e Indoor use e Temperature 5 C to 40 C e Maximum relative humidity of 85 e Maximum altitude 2000 m e Installation category II e Polution degree 2 Due to the weight of the machine all lifting and transporting must be carried out using proper handling equipme
9. Polyethylene PP Polypropylene O No information PPCO Polypropylene Copolymer PS Polysulfone d Less resistance if T gt 50 C SS Stainless steel TF Teflon t Unsatisfactory if T gt 50 C TZ Tefzel T Titanium VA VitonA Material AL BNCABCN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Acetaldehyde s U U U O 000M UMM US SMS U Acetamide 00 0 00 00085 US 50000 0 Acetic Acid 5 SMS SM S S8 SS SS DS SS SS M Acetic Acid 20 S 0 0 0 0 0 0 0 SMS 8 350000 0 Acetic Acid 60 S U U U U S SMS UM DS S SSU Acetic Acid 80 S 0 0 0 0 0 0 0 S U St S M 0 0 0 0 0 Acetic Acid Glacial SU U UU S0 OS UM DM S S S S U Acetic Anhydride S 0 0 0 0 0 0 0 Sd UU Sd U 0000 0 Acetone s U U UM M O US US MU S S M sU Acetonitrile 0 0 0 00 0 0 OM U SM U 0000 0 Acetylene S 0 0 0 0 00 085 085 S U 0 0 0 0 0 Adipic Acid 00 0 00 00 0 S 8 SS S 0 0 0 0 0 Alanine S 0 0 0 0 0 0 085 S U U 0 0 0 0 0 Allyl Alcohol 0 0 U 0 S 00 U0 SS S 00 8 ss 0 Aluminum Chloride 0 0 8 850 350 SS 8 8 GU US SS O Aluminum Fluoride 0 00 00 85 0 SSU SS 0 0 8 0 0 Aluminum Hydroxide S 0 0 0 0 0 0 0 8 M Sd 8 S 0 0 0 0 0 Aluminum Nitrate MO 0 0 0 0 0 0 S 0 0 8 0 0 0 0 0 0 Aluminum Sulphate s 0 0 0 0 0 0 0 Sd 0 8 Sd 0 0 0 0 0 0 Amino Acids S 0 0 0 0 00 085 SS 50000 0 Ammonia S 0 00 0 0 0 085 US S Sd 0 0 0 0 0 Ammonium Acetate O 0 00 0 00 085 SS 500 85 S s0 Ammonium Carbonate SUS S 0 S 0 ss US Ss Ss Ss S S O Ammonium Chloride M 0 0 0 0 0 0 O St O St St 0 0 0 0
10. and therefore its maximum allowed speed If the operator sets a speed exceeding this the centrifuge stops acceleration when the maximum allowed speed is reached ensuring the operator s safety The display will blink and an audible alarm will invite the operator to correct the speed setting to a proper value During the run the upper display will show either actual speed or actual maximum RCF values whichever is currently selected The middle display will show either remaining time timed run or elapsed time hold and pulse runs During a run the middle display will always show time The user cannot change the display to show the given radius value during a run Most set parameters can be changed during a run when the selected program is not locked Set speed can be changed by selecting speed as the control parameter for the upper display then by pushing the up or down arrow or turning the knob Set run time can be changed by pressing the up or down arrow corresponding to the middle display Set deceleration profile can be changed by first selecting the deceleration profile pushing the B button and then using the up down arrows or turning the knob When the stop button is pressed or when the run timer reaches zero the centrifuge will begin to decelerate the rotor At this point the run timer will stop and the middle display will begin to show elapsed braking time The upper display will show either actual speed or ac
11. 0 0 0 0 S Octyl Alcohol Oleic Acid S S SS SS S S 5 0 S 0 0 0 0 0 0 OM MMS Ss 0 U M M M M 00000 S S S S Orange Essence Oxalic Acid M S S S SS S S S S S 0 S S S S00 0 0 0 S 0 0 0 0 0 0 0 Oxygenated Water 20 Oxygenated Water 50 Oxygenated Water 90 Ozone S S 0 0 0 0 0 S S 0 0 0 0 0 S 0 0 00 0 0 0 8 S 0 0 00 0 0 0 0 0 0 0 0 0 0 0 Sd Sd S Sd S 00 0 0 0 S 0 0 0 0 0 0 0 S 0 0 0 0 0 0 U 000000 S U 0 0 Paraffin S 0 0 00 0 0 0 U Pentane oo wo lol lolo lo OIS lalao jo j gt jojo jo ololnalnloln zlolo ololnlnlolnlnlolo olo 5lolol lololo 51 3 33 5151515 Dj gt H gt l gt 2 m2Ii w I o gt VE S SES S SS S DIAIM i lN gt gt ololjojojoj gt jojo jo ololojojojo jojo jo ololnlololnlololo olo 5lolol5 ololo ololololololololo ololololololololo ololololol5lololo co 2 lt jon ja o jo jo as jak c c D S d olele le s KENNS Izl l S ZIT SES 23 elelele ims 2 2 2 2 slslslse slsisis S 5 s s 5 gia a gt a gt a E AS AL AL le lo Gee BE 0 S S 0 0 S S 0 0 S S S S S 0 S U 0 0 0 0 8 0 0 Phenyl Ethyl Alcohol S S 0 S S S 0M S SU Phosphoric Acid 1096 AID IO jojojo olola loo miooo jo miooo jo gt jojojojojm mio gt injojo ajd r o vV Di lalan en oli in gt
12. 0 O0 Ammonium Hydroxide 10 O SU 00 0 0 85 DU SDS SS S S S Ammonium Hydroxide conc O U U 00 OO 85 DU DO SS S SU Ammonium Oxalate 0 0 0 00 0 0 0S S SSS 00 0 0 0 Ammonium Phosphate U 0 0 0 0 00 085 MS 5S 0 0 0 0 0 0 Ammonium Sulphate ss 0 0 U 0 0 SS ss 8505 S Ss 0 Ammonium Sulphide 0 0 000 8 00 85 U 0 5005 00 0 n Amyl Acetate s 0 0 0 0 0 0 O Sd U Sd Sd U O 0 0 0 0 Amyl Alcohol S M U 0O S 00 SS S S 00 SS S M Amyl Chloride S 0 0 0 0 0 0 O U U U U U 0 0 0 0 0 Aniline S 0 0 0 0 S 0 0 U 0 S MO 0 S85 SS 0 6 4 CL40 FL40 Thermo Electron Corporation Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO 0 00 U Chemical S M 0 U U 0 OS S 0 8 000 0 0 8 000000 U U 0 U 0 0 00 0 0 0 8 U U Aqua Regia 0 Barium Chloride u 0 0 00 0 0 0 S 0 0 0 0 Barium Hydroxide Barium Sulphate 0 0 St S 0 0 0 0 0 0 0 0 S ol nlolol lololololo olwnlololnlolololole ol nlolol lololololo olwnlololnlolololole JA o o O S o o o o e SS S 4 6 3 3333 2 lD la gt a je oo lo gt AlDIn m DIGl D D DI D o SIDISIS PJP gt gt gt 2 lD a ja je lolo lo gt ol nlolol gt lololololo olololololololololeo olololololololololo o gt fjojo jojojo jojo ofjojojojojojojojo jo ojajojo j gt jojojojojo o pjojoj gt jojojojo jo AIN AlO m DID 2 2 in A o gre 2 D x3 x D c 9 9 EIE 5 213
13. you use a swing out rotor f the problem remains please contact THERMO service 08 Motor Stop with braking f the centrifuge is operated in Overtemperature good conditions and the Bad operating problem remains please conditions or contact THERMO service motor failure 09 Chamber Stop with braking If the centrifuge is operated in Overtemperature good conditions and the Bad operating problem remains please conditions or contact THERMO service cooling unit failure 10 Electronic All controls disabled Reset the device by powering malfunction it down 4 6 CL40 FL40 Thermo Electron Corporation Chapter 4 Instructions for Use Pre pa ring the First Before installation the rotor should be thoroughly inspected for Run of the Day evidence of corrosion or other damage and for cleanliness Chemical and stress corrosion of metallic parts will eventually lead to disruption of the rotor with potential severe damage to the centrifuge Particles stuck inside the pockets can cause breakage of tubes and lead to major imbalance and or loss of sample and contamination The central hole of the rotor and the drive spindle should also be clean and undamaged These parts should be wiped clean before each use mn Carefully check the tightness of the rotor locking nut Re tighten if necessary A The centrifuge also should be observed to verify proper appearance of screws lid hooks latches and for evidence of corrosion In case of proble
14. 0 FL40 Certificate of Decontamination and Cleaning Read carefully the instructions below before sending an instrument or parts of it to Thermo Service Dept or to any Authorized Technical Assistance Service A Mr Mrs name sss Establishment A a em ge en oan ae ee as Address POSE ZIP COU MN Hg E State Country Declares the cleaning and decontamination of the following Product tyDG c meter roer Serial N b ie Serial N iin D EA Serial N Accessory DeSeriDllOn tee ote nete E Serial N ACCESSORY DE SEL D LO cerco perfe en eene Serial N Nature of contamination Decontamination certified by IW EEN SO cce eere PV ALE M pm Signature When an instrument or parts of it requires servicing by Thermo personnel the following procedure must be accomplished to ensure personnel safety e Clean the instrument and proceed to its decontamination from any kind of dangerous products e Compile this Decontamination Certificate with all the information required Thermo Electron Corporation Thermo Electron Corporation Certificate of Decontamination and Cleaning e Attach this Certificate to the instrument or part before sending it to Thermo Service or other authori
15. 010 2 020 international standard which specifies particular safety requirements for laboratory centrifuges According to the standard warnings the operator must respect the following precautions Mark a boundary area of 30 cm around the centrifuge No person or hazardous material can stay within this boundary longer than necessary for operational reasons while the centrifuge is operating An emergency switch for disconnection of the mains in case of malfunction has to be available and familiar to all the persons operating the centrifuge This switch has to be remote from the laboratory centrifuge preferably outside the room in which the centrifuge is located or adjacent to the exit from that room Moving or shifting the machine during centrifugation is dangerous Using the centrifuge with rotating equipment showing evidence of corrosion and wear marks manipulating and or tampering with the electronic and mechanical parts are dangerous operations Never try to bypass the lid lock safety while the rotor is spinning Do not try to open the lid until the display returns to the stand by mode Only use a correctly grounded power source Use only rotors and accessories designed for use in these centrifuges A Pay special attention to the following Installation of the unit proper ventilation leveling of the centrifuge rigidity and stability of the support Thermo Electron Corporation Speed Control Operational
16. 3291 4500 4166 174188 Beige d 36 8 20 10 flat 172 4000 3077 4500 3894 11174161 Brown 7 28 17 7 134 15 vac 185 4000 3309 4500 4188 177573 Black 12 48 8 22 15 con 188 4000 3363 4500 4256 174190 Orange 12 48 6 5 25 15 184 4000 3291 4500 4166 11174163 Coral 12 48 222 108 20 177 4000 3166 4500 4007 174532 Black 7 28 25 5 93 5 25 flat 173 4000 3095 4500 3917 174186 Dark red 7 28 30 15 25 flat 172 4000 3077 4500 3894 11174164 Dark red 7 28 30 115 50 182 4000 3256 4500 4120 174225 Black 3 12 35 5 20 50 184 4000 3291 4500 4166 174529 Black m 28 30 16 50 con 185 4000 3309 4500 4188 11177572 Black 5 20 30 116 50 con 4000 3363 4500 4256 174199 Light blue 4 16 30 20 50 flat 172 4000 3077 4500 3894 174165 Dark blue 4 16 35 20 50 185 4000 3309 4500 4188 11174166 Dark green 2 8 45 118 100 185 4000 3309 4500 4188 174167 Turquoise 4 63 34 250flat 185 4000 3309 4500 4188 174227 Black 4 65 300flat 185 4000 3309 4500 4188 11174192 Navy blue 4 80 5 125 450 185 4000 3309 4500 4188 174202 Black 4 Cytoblock 180 4000 3220 4500 4075 174203 4 600 flat 190 4000 3399 4500 4302 See Corning for adapters 4 500 con 190 4000 3399 4500 4302 81005750 Bottle set of 6 4 100 133 750 178 4000 3184 4500 4098 3 6 CL40 FL40 Thermo Electron Corporation Chapter 3 Installation CL40 FL40 CL40R FL40R Cat N Color of insert Insert Rotor Max Tube Max Tube Max Max Max Max Max setof4 ortype Capacity Capacity mm tube height volu
17. C PE PP PS SS TF TZ TI VA Chemical PPCO Rubidium Bromide M 0 0 0 0 0 0 085 S 0 S 00000 0 Saccharose U 0 0 0 0 0 0 085 SS 5 50000 0 Salycylic Acid Sat 0 0 0 0 0 00 0 S dS S S 0 0 0 0 0 Serum S 00 00 00 085 ss ss 0 Ss SU O0 Silver Acetate 0 0 0 0 0 00 0 S dS S S 00 0 0 0 Silver Nitrate U 0 0 0 0 00 085 S S S 80 s0 0 Sodium Acetate S 0 0 00 0 0 0 SSS S Ss 0 0 0 0 0 Sodium Bisulphate MO SS 0 5S 5S SS SS 505 85 05 0 Sodium Borate MoO 0 0 0 0 0 O S S Sd S 0 0 0 0 0 0 Sodium Bromide U 0 0 0 0 00 085 S 0 S0 0 0 0 0 0 Sodium Carbonate 2 MS S S8 8 Ss Ss S8 S S S D O S S S S S Sodium Chloride 1096 S S 58 50058588 SSS 8 SS SM S Sodium Chloride Sat d S S 0 0 S 00 S S 0 8 SOSS S S S Sodium Hydroxide gt 1 UMS SU SOS S US SS SS SSS Sodium Hydroxide 10 U MU UU 0 SSU SS Ss bb Ss Ss S Sodium Hydroxide conc U M U U U 00 0M U S MO S S Ss MU Sodium Hypochlorite 5 MMS S 5S SS D S amp S S 5S 5S M 5S SS Ss Sodium lodide MoO 0 0 0 00 0 S SO S 0 0 0 0 0 0 Sodium Nitrate S 0 0 00 0 0 0 S 0308 00000 0 Sodium Sulfate S 0 0 0 0 0 0 0 Sd O Sd Sd 0 00 0 0 O Sodium Sulphide SS S 0 0 8 0 SSU S S 0 Ss Ss SM SS Stearic Acid S 0 0 0 0 0 0 0 S Sd Ss ss 0 0 0 0 0 Sulphuric Acid 10 MU S SU SS SS MSS SUS SS S Sulphuric Acid 2096 U 0 0 0 0 0 0 0 Sd Sd S Sd S 00 0 0 0 Sulphuric Acid 50 U U U U U S S U S S S S S US SM S Sulphuric Acid conc U U U U U S 0 U D U M D UMS U Sulphuric Anhydride dry or moist S 0 0 0 0 0 0 0 SSS 5 5 0 0 0 0 0 Tetrac
18. IEC CL40 FL40 Series User s Manual re LM li cau ty es L L Analyze Detect Measure Control ELECTRON CORPORATION IEC CL40 FL40 Series User s Manual CL40 FL40 Series Thermo ELECTRON CORPORATION Carefully read this manual before operating your instrument Information contained in this document is the property of Thermo Electron Corporation it may not be duplicated or distribuited without the owner s authorization The validity of the guarantee is subject to the observation of the instructions and precautions described in this document Preface MANUAL NUMBER REV ECR ECN DATE DESCRIPTION BY a 11290088 03 06 Initial release Thermo Electron Corporation CL40 FL40 i Packing List LI 11210922 CL40 230V 50 60 Hz 11210940 FL40 230V 50 60 Hz L 11210923 CL40 120V 60 Hz C 11210941 FL40 120V 60 Hz LI 11210924 CL40 100V 50 60 Hz 11210942 FL40 100V 50 60 Hz LI 11210926 CL40R 230V 50 60 Hz 11210943 FL40R 230V 50 60 Hz L 11210927 CL40R 120V 60 Hz C 11210944 FL40R 120V 60 Hz LI 11210928 CL40R 100V 50 Hz 11210945 FL40R 100V 50 Hz LI 11210929 CL4OR 100V 60 Hz 11210946 FL4OR 100V 60 Hz Item number Ouantity Check Centrifuge LI 11290088 User manual 1 LI CE Declaration of compliance L Manual lid opening instructions 1 LI Imbalance protection instr
19. Limitations Aerosol Risks Thermo Electron Corporation Chapter 5 Hazard Precautions and Limitations of Use e Rotor installation verify that the rotor is locked in position before use e Cleaning of the accessories and of the rotor chamber is particularly necessary when corrosive products are present in the samples saline acids bases e Load balancing IF ANY DANGEROUS EVENT OCCURS KEEP YOURSELF FAR AWAY FROM THE INSTRUMENT AND SWITCH OFF THE MAIN POWER SOURCE A view port located on the lid allows the measurement of the actual speed by a phototachometer Once a year check for correct r p m readout and speed control setting which should be within 100 r p m of the actual The CL40 and FL40 series of centrifuges and their associated rotors offer a high level of RCF performance In some cases the maximum possible RCF will exceed the one sustainable by the sample container The rotor RCF performance displayed is the maximum available in the rotor pockets Depending on the sample containers or on other application needs the maximum RCF which must be used is to be determined by the operator The centrifuge is not explosion proof Using this centrifuge with explosive samples is entirely at the user s own risk Do not under any circumstances use the centrifuge in an explosive environment A Due to the action of the turbulence created in the bowl a centrifuge is an aerosol generator A ventilated centrifuge like
20. Mt Mt S SM SS S 0 00000 U S 0 0 0 0 0 0 0 Mt S 0 Cyclohexane 0 S 0 S 0 0 S U 0000 Cyclohexanol U 0000 U 0000 U U U U U U U U 0 0 0 0 0 0 0 0 Mt 0 0 0 0 0 0 0 0 0 0 Cyclohexanone Cyclopentane Decane 0 0 0 Mt 0 0 0 Mt Mt Mt Mt Sd 0 0 0 0 0 S S 0 S 500 S 0 0 S S M 0 0 0 0 00 0 S S 0 Dextran Sulphate Diacetone 0 S 0 O 000 S0 S 0 U 0 85 000000 S 0 0 00 0 0 0 Diacetone Alcohol ololhlolololnlolololololo l gt ololnlolololnlololnlolnlnlu ololnlololol nlololzlo ls sjle ololnlolololnlololnlolnlnlu ololololololololololololmlo jojojo je jo jo gt jo jo jo S S gt lol s5 jm ln gt lulu leo LS ele S Ss 5 5 5 5 l lolszlhbhiwlolz 5555 S5 Sas se 5 5 5 E SIS ol loja la lo gt jm jam js olol nlololol gt lolol5lolololo ololnlololololololololololo ololololololnlolololololole ololnlolololnlololslololols olo 5lololol nlololslolololo olo 5lololol nlololslololols olo 5lololol nlolololololols ololololnlolnlolnlnlolnlnlw D ES LLI gt ES oo 2313 55 e 8 8 E EIE oO BIO SEE elajsle o olc gz g mico coit pIe GAEREN MEA ele ala 212 4 2 afje o ejyejejejo _ o CH el bf z z o elalaleolzisis sss 8 8 E8 E SSL lc lc lS lsS S S S S V D V S oloalsfelelele o e E E S olalalalalalalalalalalalald 0 0 0 U 00 U 00 0 0 0 0
21. S S U 00000 S S Mt 0 0 0 Mt S 0 S S S U Fluorhydric Acid 1096 Fluorhydric Acid 5096 M U S S MUS S 0 0 S U U U Thermo Electron Corporation CL40 FL40 6 6 Chapter 6 Service and Maintenance Material BN CAB CN DL KY NO NY PA PC PE PP Chemical PPCO Formaldehyde 20 S Sd Formaldehyde 4096 S S Formaldehyde 50 S Sd Formic Acid 10096 S M Freon TF Sd Sd Fuel Oil Sd Glucose Glutaraldehyde Glycerine Glycerol Heptane Hexane Hydrazine Hydrochloric Acid 596 Hydrochloric Acid 37 96 Hydrochloric Acid 5096 Hydrochloric Acid conc Hydrofluoric Acid 1096 Hydrofluoric Acid 5096 Hydrogen Peroxide 396 Hydrogen Peroxide 10096 lodine Crystals Isobutyl Alcohol Isopropyl Alcohol Isopropylbenzene Kerosene Lactic Acid 2096 Lactic Acid 10096 Lead Acetate aq Lemon Essence Lime conc Magnesium Chloride Magnesium Hydroxide Magnesium Nitrate Magnesium Sulphate Manganese Salts Mercury Methanol 10096 Methyl Acetate Methyl Alcohol 10096 Methyl Butyl Ketone Methyl Ethyl Ketone Methyl Isobutyl Ketone Methyl Isopropyl Ketone Methylene Chloride Mineral Oil Nickel Chloride Nickel Salts gt E U e SS TF TZ TI VA co EL co co a o co e co e co e o co c olululululolulolculu zlu C C C C C co co min aa Z ol ojl a a
22. apter 3 Installation 11174154 Standard buckets 4 Inserts see table 41193190 750 ml bottles 6 85240471 Cushion 4 for 750 ml bottles 62813600 Standard buckets 4 PTFE coated Inserts see table 41193190 750 ml bottles 6 85240471 Cushion 4 for 750 ml bottles 11174218 Sealed buckets 4 Inserts see table 41193190 750 ml bottles 6 85240471 Cushion 4 for 750 ml bottles 25144058 Spare sealing lid certified biocontainment 85241381 Spare seal 62814801 Sealed buckets 4 PTFE coated Inserts see table 41193190 750 ml bottles 6 85240471 Cushion 4 for 750 ml bottles 25144058 Spare sealing lid certified biocontainment 85241381 Spare seal 11175372 Bucket for CORNING 250 ml conical bottles 11175378 Sealed bucket for 250 ml conical bottles 11175385 Sealed bucket with liner for blood bags 11175370 Bucket for Nalgene 500 ml flat 11175371 Adapters 4 for 250 ml conical bottle in 11175370 85240513 Adapters 4 for 2 x 60 ml in 11175370 11210823 Microplate carriers 4 11174207 Rubber cushion for flexible microtiter plates 11174223 Sealed microplate carriers 4 11174207 Rubber cushion for flexible microtiter plates 11174203 600 ml Stainless steel bottles 4 41176423 Lid for 600 ml bottle Cytology accessories for use with the insert 11174202 11172499 Cytobuckets 2 1203558 Cytobucket slides 50 1203559 Cytobucket foam layers 50 1203561 Tube of silicone 1203562 Blotti
23. bulence solid particles accidentally left in the centrifugation chamber create excessive wear of the chamber itself and of the outer rotating equipment surface A To install the rotor e Check that the motor shaft and rotor are clean e Put the rotor on the motor shaft e Secure the rotor on the shaft by turning the hexagonal nut counter clockwise with the supplied tool e Verify that the rotor cannot be removed by trying to pull it gently off the shaft e In case you are using a swing out rotor e Gently grease the trunnions Put the buckets on the trunnions after verifying that the weight of the buckets is identical the weight is engraved on the bottom of each bucket In the event of mains non availability or power failure opening of the lid is prevented by the lid locking safety device It is recommended that the user waits for the mains to be switched back on so that this safety device enables the lid to be unlocked Manual lid unlocking must only be done by someone informed of the possible danger and of the necessary precautions Rotating parts are a risk as they could come in contact with the user or be ejected There is particularly high risk of injury if e The user attempts to manually stop the rotor CL40 FL40 3 3 Chapter 3 Installation e Any object falls inside the centrifugation chamber while the rotor is running A Manual lid unlocking may be necessary under a very limited number of conditions suc
24. dentification label Cat N Content Voltage Frequency Type Keypad control 11177560 CL40 1177563 CL40R 10 A single 1177566 FL40 ground 1177569 FLAOR 1177561 CL40 1177564 CL40R 1177567 FL40 ground 1177570 FLAOR For your safety check that mains wiring is effectively grounded Thermo declines all responsibility for any damages due to non grounding of the machine A Remember that in order to respect the electrical safety standards related to protection against indirect contact the supply of power to the instrument must be via a power socket fitted with a protection device ensuring automatic cut off in the case of an insulation fault A supply fitted with a circuit breaker of the correct rating complies with this requirement Thermo Electron Corporation Lid Opening and Rotor Checking Manual Lid Unlocking Procedure Thermo Electron Corporation Chapter 3 Installation Ensure that the centrifuge has been switched ON Pull the handle on the centrifuge right side towards the front of the unit the lid is automatically unlocked and opens In the case of a mains power outage opening of the lid is prevented by the lid lock safety device It is recommended to wait for the mains power to be switched back on so that this safety device enables the lid to be unlocked refer to 3 6 for manual lid opening Carefully clean the inside of the centrifugation chamber removing any packing residues In fact due to air tur
25. dicator LED 14 is illuminated the lid interlock is not active The lid can be opened The indicator will only be illuminated when the rotor is at rest and it is safe to open the lid Q m When the lid interlock indicator LED is not illuminated the lid cannot be opened The rotor is still in motion This feature is in accordance with international safety standards When the load imbalance indicator LED 13 is illuminated the centrifuge run has been A automatically ended due to excessive load imbalance The rotor will be decelerated to rest at Q A which time the lid can be opened and the load can be re balanced When the lid is opened this indicator will go off 4 4 CL40 FLAO Thermo Electron Corporation Description of Certain Events with Respect to the Control System Start and acceleration phase Set error During the run Stop and braking deceleration phase Thermo Electron Corporation End of run Chapter 4 Instructions for Use When the start button is pressed the centrifuge will begin to accelerate the rotor At this point the run timer will start and the middle display will begin to show remaining run time The upper display will show either actual speed or actual maximum RCF values whichever is currently selected The lower display shows the given acceleration profile and the LED corresponding to the acceleration button is illuminated During acceleration the centrifuge automatically detects the rotor type
26. e puller supplied position its screw above the motor shaft and position the puller arms to catch beneath the rotor hub e With the wrench supplied tighten the puller screw clockwise against the motor shaft until the rotor loosens Trunnion Lubrification This operation is necessary to allow the buckets to swing freely Clean the trunnions regularly with a dry wipe as well as the part of the bucket that rotates on the trunnions Then put a very small quantity of grease on the curved face of the trunnion Do not apply too much grease because it will eventually coat the bowl of the centrifuge as centrifugal force pulls the grease from the trunnions If the centrifuge is having imbalance problems try this operation uj before calling Thermo service In many cases imbalance problems arise due to the fact that poorly maintained bucket trunnion interfaces prevent the buckets from swinging freely A Thermo Electron Corporation CL40 FL40 6 3 Chapter 6 Service and Maintenance Chemical Compatibility Table for Rotors Tubes and Accessories S Satisfactory AL Aluminum BN BunaN D Discoloration but OK CAB Cellulose acetate butyrate CN Cellulose Nitrate P Pure chemical OK DL Delrin KY Kynar M Moderate resistance NO Noryl NY Nylon U Unsatisfactory PA Polyallomer PPCO PC Polycarbonate X Explosion risk PE
27. etting automatically applying the wrong RCE Thermo Electron Corporation Chapter 2 Specifications Dimensions and Weight CL40 FL40 CL40R FL40R Product Dimensions Height lid open cm in 92 36 2 134 58 8 92 36 2 134 58 8 Height lid closed cm in 42 16 5 86 33 8 42 16 5 86 33 8 Width cm in 58 22 8 58 22 8 85 33 5 58 22 8 Depth cm in 66 26 0 66 26 0 66 26 0 66 26 0 Weight unpacked kg Ib 80 176 95 208 106 233 115 253 Packing Height cm in 50 19 7 99 38 9 50 19 7 99 38 9 Width cm in 70 27 6 68 26 7 97 38 2 68 26 7 Depth cm in 67 26 4 78 30 7 67 26 4 78 30 7 Weight packed kg Ib 91 201 120 264 116 256 145 319 Centrifugation Specifications CL40 FL40 CL40R FL40R Max capacity Swing out 4x750 mL 3000 mL 4x750 mL 3000 mL Fixed Angle 8x100 mL 8x100 mL Max density 1200 kg m3 1200 kg m3 Max load 3 6 kg 3 6 kg Max speed Swing out M4 4000 rpm 4500 rpm Swing out 062 5 5400 rpm 5400 rpm Fixed Angle 7800 rpm 8000 rpm Max RCF Swing out M4 3810 xg 4822 xg Swing out 062 5 4140 xg 4140 xg Fixed Angle 9862 x g 10375 x g Max noise lt 66 dBA lt 62 dBA CL40 FL40 CL40R FL40R Microprocessor control yes yes Programs direct recall 5 5 Speed set display Range 500 7800 500 8000 Step 10 rpm at less than 1000 rpm 10 rpm at less than 1000 rpm 100 rpm at above 1000 rpm 100 rpm at above 1000 rpm Accuracy 20 rpm display
28. fying cross checking and monitoring the results under different conditions of temperature pressure and purity relating to the solvents and samples dissolved therein It is strongly recommended that you carry out your own trials particularly before protracted work periods 6 10 CL40 FL40 Thermo Electron Corporation Thermo Electron Corporation Chapter7 Disposal of Product The following disposal recommendations are valid for the machine or its accessories at the end of its useful life or the parts that be disposed f because they are worn out or degraded during use Prior to disposal the machine and accessories must be decontaminated In this respect the user is required to submit the enclosed decontamination certificate completed and signed to the company or persons who are in charge of disposal The elements of the product that may become noxius during the life of the product are the folowing e Electric batteries and accumulators situated in the front panel of the machine among the other electrical and electronic componenets e Oil in the compressor of the refrigeration systems e Refrigerant Disposal of these elements must be in accordance with applicable regulations and recommendations defined by the relevant regulatory bodies In additions these elements must be disposed of differently based on the different noxious substances they contain possibly by a specialized disposal company CL40 FL40 7 1 1 1 CL4
29. h as the urgent recovery of critical samples that could be damaged if left in the centrifuge rotor In this case the lid can be opened by using the special tool supplied with the centrifuge ER Always set the power switch to the OFF position before performing this manual procedure even in the case of a mains power outage A Should the emergency occur due to power failure during centrifugation wait at least 10 minutes for the end of the rotor rotation In spite of the absence of noise the rotor could still be rotating when you need to open the lid manually Upon opening lift the lid by hand and observe the rotor be careful in this operation if it is still rotating close the lid and wait 7 p Figure 1 Insert the unlocking tool horizontally into the hole on the right hand side of the instrument Figure 2 While pressing up with your finger to press down with the unlocking tool pull the lid lever toward you the lid opens upwards The protection sticker must be replaced after it has been pierced item N 85241772 a ES Percericiavec l outil E N Pierce here with tool N E N BN S gt lt di 3 4 CL40 FL40 Thermo Electron Corporation Performance and Accessories M4 Swing out Rotor Cat N 11175338 M4 swing out rotor 4x750 mL e 4500 rpm 4822 x g e For 4 750 mL bottles Up to 148 5 mL tubes Up to 12 standard microplates e Certified sealed accessories are available Ch
30. he action of opening the lid itself causes the dispersion of aerosols into the environment When an elevated risk of aerosols or of breakage is perceived the accessories should be handled using gloves and opened in an environment ensuring the protection of the operator safety cabinet glove chamber wearing a mask even if they are apparently sealed A Thermo Electron Corporation Periodic Cleaning Daily Weekly Contamination Thermo Electron Corporation Hazards ca Chapter 6 Service and Maintenance Chapter 6 Service and Maintenance All cleaning operations should be performed with the centrifuge disconnected from the power outlet A No daily cleaning is required except in the case of accidental tube breakage or when there is a large amount of spillage in the bowl Clean the bowl and the accessories with a cotton wool pad dipped in 70 alcohol Never use metallic brushes to clean accessories e After cleaning the accessories rinse them in running water preferably distilled water e Dry the rotor with a soft absorbent non woven cloth or tissue Drying may be finished off with a warm air jet e g a hair dryer e Make sure that the pockets are well dried e Ideally spray with Anti Corrosion Spray Cat N 11175399 Store unused buckets upside down on a non metallic grid to allow free passage of air The centrifuge is likely to be used in medical research where hazardous substances including rad
31. he main power supply Coming in contact with high voltages could cause severe injuries A In this manual this symbol means that you will find important information for safety which if unobserved could result in damage to the appliance and or harm to the operator A In this manual this symbol means that you will find important information about minimizing biological risk if unobserved the result could be harm to the operator A The CL40 and FL40 centrifuges are designed for laboratory use They will separate the components of fluids into layers of varying density by subjecting them to high forces Centrifugal force provided by the centrifuge can also be used to drive solvents and low molecular weight solutes through the membrane of a filtering device Retained macrosolutes will therefore be found above the membrane Relative Centrifugal Force RCF generated by a rotor is directly CL40 FL40 1 1 Chapter 1 Use and Function 1 2 Refrigeration and Heating Systems Condensation drain on CL40 FL40 refrigerated units proportional to its sedimentation useful radius and to the value of its speed squared A control system permits the user through an extremely intuitive control panel to set and control the speed the g force the temperature on the thermostated and refrigerated versions and the run time as well as to view different messages and warnings The CL40 and FL40 are ventilated unit A permanent air circ
32. hloroethane M 0 0 0 0 00 0M 00M 0 0 0 0 0 0 Tetrachlorethylene 0 0 00 0 0 0 0U 08 U 0 0 0 0 0 0 Tannic Acid MoO 0 0 0 0 0 0 S 0S S 0 0 0 0 0 0 Tartaric Acid MO 0 0 0 0 0 0 S Sd S S S 0 0 0 0 0 Tetrahydrofuran s 0 0 0 0 0 0 OU UU U UO Ss 00 Thionyl Chloride 0 0 0 0 0 0 0 OU U U U U 0 0 0 0 0 Toluene SUP SM S 0 UU UU U Uz SMM Trichlorethylene SU 000 Ss 0 UU UU U U U SM S Trichloroacetic Acid U 0 0 0 0 0 0 0 M Mt Mt M S 0000 0 1 2 4 Trichlorobenzen 0 0 0 0 0 0 0 OU UUUUO0OO 0 0 0 Trichloroethane SU S 0 M S0 SU UU UM OS SS S Triethylamine 0 0 0 0 0 00 O U 0 0 U 0 0 0 0 0 0 Triethylene Glycol 0 0 00 0 0 0 0 SSS ss 0 0 0 0 0 Tris Buffer neutral S 0 0 00 0 0 OS S S S SOS S 0 0 Trisodium Phosphate 0 0 S 0M 00 8 50 8 00 5 5 5 S Triton X 100 S 0 0 00 00 085 SS Ss 85 0 S MO O Turpentine S 0 0 0 0 0 0 O Sd Mt St Sd U 0 0 0 0 0 Undecyl Alcohol 0 0 0 0 0 0 0 0 Sd Sd St Sd M 00 0 0 0 Urea MoO S SS 00 S S S S S Ss SS Ss S0 Urine 00 SOS 00 SS Ss Ss 0 0 8 Ss s0 Vinylidene chloride 0 0 0 0 0 0 0 0U UU U U 0 0 0 0 0 Thermo Electron Corporation CL40 FL40 6 9 Chapter 6 Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Xylene SU P OM S O U U U U OU US SS S Zinc Chloride M S S 00 00 SS SS S85 SMS SS S Zinc Hydrosulphite U 0 0 0 0 0 0 00 00 0 0 0 0 0 0 0 Zinc Sulphate U 0 0 0 0 0 0 0 Sd O S Sd 0 00 0 0 0 This table is intended as a guide only because of the difficulty in quanti
33. ioactive chemicals are frequently found ALWAYS USE THE APPROPRIATE DECONTAMINATION PROCEDURES WHEN THE ROTOR IS EXPOSED TO THESE CHEMICALS A Examples of commonly used techniques are outlined below The information is given as a guideline only It is the responsibility of the owner to use the most suitable procedure If decontamination procedures require the use of warming i e autoclave the rotating equipment should always be completely disassembled before being subjected to heat and after external chemical cleaning Seals should be decontaminated with the method most suitable for them CL40 FL40 6 1 Chapter 6 Service and Maintenance 6 2 Disinfection Radioactive Decontamination CL40 FL40 Alcohol 70 ethanol or isopropanol applied for 10 minutes is ideal for bacteria and viruses Autoclave for 20 minutes at 120 C to destroy micro organisms Polypropylene components are autoclaveable Rotor lids must be disassembled from rotor bodies O rings autoclaved separately should be replaced when deformed Any part which has been subjected to temperatures above 130 C must be discarded Hypochlorite bleach used at 0 1 concentration with 10 minutes immersion is effective against bacteria spores and viruses but as an oxidizing agent is corrosive to metal alloys and must be thoroughly rinsed off of metal parts and the parts dried It should never be used if there is surface damage to the rotor
34. l a z L al cl al zZ oa a L oa L Ll ono o co co alu FS an ni a aj oz Lj a cj a za ne o E lt Z O C ml wm nl ol ol nl wml COOC wm Go co Gol oO A wm wm Oo Z vn Zlol Zl l_ cl cl of Of mH Of C O WH wm toi co E Z olal J yy a al wm oO Sly a g Sf yn a Ch a oO a a Cc z an a a a wm zgouic ov ov C Cc Cc One Mm WM Ol CO Ol OF S DM CO CO CO O XM ol Of C OF CO C GO MW DG MW GD ol GD ol ol D O DO OD WM wm GD Oo JZ Z oj Cc z c CP KT calo ol olol ol olol ol ol oj Z o z o njojojejojojec ol ol jojo opjojcjojojo opjojojo oO Cl Cc Of ym jo Cc SZ Cc clololololololololololol Clo alo ol o O c oj oj c ol clol c ol ol ol ol olol ol olol ol olol ol cl ol oj al alj c lt lolclolololololvewlolololololololelo ojojojajojol z oj JZ CO z olol olol ol APO ol ol ol olol ojo al oj El al c El KC clololololololololololol cl ol oj o olol olal olol JZ olal olal ojo jojo Mm DP OD GD AD ol ol D O DJL DJ olol ol DY n olal DG D O GD OD al DG DG olol ol oal olojo ol ol olololololololololololololelolololololelolol vwlololelelolololelolololololvelolololololelolelo w ol olclololclolclolclolololololololololololololc clololelol vw ololololololololololololol cfolelo wvlolclalalalclolzlealaz clolololalelcalolelulelela lzlwclelela lzlcalalaclelalololelelgo XN ol olwlolol
35. me Radius Speed RCF Speed RCF mm mL mm rpm xg rpm xg 11174169 1 4 Blood bag 190 4000 3399 4500 4302 pediatric 11174223 Carriers 3 12 Microplates 173 4000 3095 4300 3917 sealed 11210823 Carriers 4 16 Microplates 173 4000 3095 4500 3917 11175372 Buckets 1 4 Corning 250 mL 250 con 213 4000 3810 4500 4822 11175378 Sealed buckets 1 4 Corning 250 mL 250 con 213 4000 3810 4500 4822 11175385 Sealed buckets 1 4 Blood bags 190 4000 3398 4500 4301 11175370 Buckets 1 4 Nalgene 500 mL 500 flat 208 4000 3721 4500 4709 outer rings of holes n open buckets only Inserts Available for the 11175370 Bucket for Nalgene 500 ml Flat Cat N Color of insert Insert Rotor Max Tube Max Tube set of 4 or type Capacity Capacity mm tube height volume mm mL 11175371 1 4 250 con Thermo Electron Corporation Max Radius mm 208 CL40 FL40 Max Max Speed RCF rpm xg 4000 3721 All holes filled in sealed buckets Smaller quantities of taller tubes may be accommodated by omitting the CL40R FL40R Max Speed rpm 4500 CL40 FL40 Max RCF x gl 4709 3 7 Chapter 3 Installation Biocontainement Accessories Biological spills outside biological safety cabinets will generate aerosols 7 that can be dispersed in the air throughout the laboratory This is particularly true when using ventilated centrifuges These spills can be very serious if they involve microorganisms o
36. mm e min max 93 110mm 8000 rpm 10375 x g e 35 angle round bottom Thermo Electron Corporation CL40 FL40 3 9 Chapter 3 Installation Glassware Capacity Material Bottom Type Standard Graduated Insert Rotor tube Cat No tube Cat No Cat No model 12m Glass conical 0 41191314 41191315 11174163 12m Glass round C 41191212 174163 16m Glass round 0 41191110 41192111 174163 24m PP round E 41193113 11174163 20m Glass conical C 41191238 174165 27m Glass conical 0 41191336 41192337 1174151 CD2 40m Glass round E 41191232 11174165 CD2 47m Glass round 0 41191130 41192131 174165 CD2 58m PP round C 41193134 174165 CD2 66 m Glass conical G 41191466 11174166 CD1 95 m Glass round C 41191262 174166 CD1 104 ml Glass round 0 41191160 174166 CD1 130 ml PP round b 41193164 11174166 CD1 375 ml Glass round 0 41191186 174192 485 ml PP round C 41193189 174192 PP Polypropylene O Open C Capped 3 10 CL40 FL40 Thermo Electron Corporation Chapter 4 Instructions for Use Controls and Indicators All the controls are located on the front panel The front panel is an intuitive interface no previous operational knowledge is necessary every parameter can be set by pressing the cursor key or rotating the knob from the minimum to the maximum and viceversa All parameters can be accessed and changed both during operation and while the machine is at rest Keypad version ELECTRON CORPORATION
37. ms contact your Thermo Service representative because any deviation from the above mentioned advice may have serious consequences for the safety of the appliance and of the operators A yx Normal use of the centrifuge could require the manipulation of Y biohazards Users and service personnel must have specific training for each substance they use according to the Laboratory Biosafety Manual from the World Health Organization A Sample Loading The contents of each rotor pocket including sample tube cap and adapter where used must be of the same weight as the one diametrically opposite Imbalance of the rotor may cause major damage to the rotor and centrifuge A Do not attempt to introduce liquids into rotor pockets or into tubes already in the pockets Distribute the load correctly Loads should be inserted two by two samples of equal weight at 180 degrees from the rotation axis and at equal distance radius from the center of rotation see pictures When using fixed angle rotor weight deviation between the tubes must not exceed 5 grams Thermo Electron Corporation CL40 FL40 4 7 Chapter 4 Instructions for Use Correct When using a swing out rotor the samples are placed in plastic inserts The load must be statically and dynamically balanced e Static balancing consists of balancing the weight of the 2 inserts which are in opposite locations in the rotor 15 to 20 grams e Dynamic balanci
38. n program The value of the acceleration profile is displayed either during acceleration or when the acceleration set button 8 is pressed The value of the deceleration profile is displayed either during deceleration or when the deceleration set button 9 is pressed Right screen 17 on the R units This screen displays either the acceleration or the deceleration profile selected for the given program The value of the acceleration profile is displayed either during acceleration or when the acceleration set button 8 is pressed The value of the deceleration profile is displayed either during deceleration or when the deceleration set button 9 is pressed In addition it shows the temperature in the bowl of the centrifuge When the centrifuge is at rest the set bowl temperature is displayed C During a run the actual bowl temperature is displayed C 4 2 CL40 FL40 Thermo Electron Corporation Chapter 4 Instructions for Use Control Panel Functions Function Start cycle Keystroke sequence and description Press the start button 12 Stop cycle immediate Press the stop button 10 Pulse Press the pulse button 11 and hold The centrifuge will accelerate the rotor to the speed setting of the active program When the button is released the rotor will be decelerated to rest Set speed When the LED next to RPM 18 is illuminated the upper display shows speed values To increase decrease the speed val
39. nformit tserkl rung Wir Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France erkl ren dass das hier beschriebene Ger t sich in bereinstimmung mit folgenden europ ischen Richtlinien befindet Declaraci n de conformidad Nostros Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que el instrumento bajo descrito est fabricado seg n las especificaciones establecidas por las siguientes normas europeas Declaracao de conformidade N s Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que o instrumento aqui apresentado est de acordo com as normas europ ias abaixo listadas Dichiarazione di conformit Noi Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France dichiariamo che lo strumento sotto descritto conforme alle seguenti Direttive europee e Machinery 98 37 CEE Low voltage 72 23 CEE 93 68 CEE e EMC 89 336 CEE 92 31 CEE 93 68 CEE e Safety standard EN 61010 1 EN 61010 2 020 Ch teau Gontier 28th June 2005 Thermo Electron Corporation CL40 HAD v Guarantee Terms Thermo guarantees that this unit is free from defects in materials and workmanship when it leaves the factory and will replace or repair the unit if it proves defective in normal use or during service for a period of ONE YEAR from the delivery This gua
40. ng consists of placing the tubes in each insert symmetrically according to the axis of the trunnions Dynamic Balancing Correct E Incorrect If less than the maximum complement of samples is loaded the tubes must be placed in opposite pockets An odd number of tubes requires an additional blank water filled tube of identical total weight to balance Significant vibrations can damage the accessories the unit and the samples Most vibration is caused by incorrect loading of the tubes For 4 8 CL40 FL40 Thermo Electron Corporation Thermo Electron Corporation Chapter 4 Instructions for Use this reason the centrifuge is equipped with an imbalance detector In case of excessive imbalance the load imbalance indicator LED is illuminated and the brake is applied immediately The rotor will be decelerated to rest in few seconds As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides Also remember that the pins must be softly lubricated Inadaquate lubrication of the pins may cause incorrect swinging of the buckets resulting in imbalance phenomen See chapter 6 6 for details If the message persists despite your efforts to balance the load call your Thermo Service representative CL40 FL40 4 9 5 1 IEC 1010 2 020 CL40 FL40 Cautions Chapter5 Hazards Precautions and Limitations of Use This centrifuge conforms to the IEC 1
41. ng paper pad 1 1 1 1 Thermo Electron Corporation CL40 FL40 3 5 Chapter 3 Installation Inserts Available for the Following Buckets 11174154 Standard buckets 4 62813600 Standard buckets 4 PTFE coated 11174218 Sealed buckets 4 62814801 Sealed buckets 4 PTFE coated CL40 FL40 CL40R FL40R Cat N Color of insert Insert Rotor Max Tube Max Tube Max Max Max Max Max setof4 ortype Capacity Capacity mm tube height volume Radius Speed RCF Speed RCF mm mL mm rpm xg rpm xg 174204 Black light blue 30 120 8 5 40 0 8 165 4000 2952 4500 3736 174201 Black light blue 30 120 1 5 40 15 2 160 4000 2862 4500 3622 11174206 Black coral 12 48 16 5 40 4 flat 184 4000 3291 4500 4166 174205 Black beige d 36 8 40 4 flat 184 4000 3291 4500 4166 174216 Blac 16 64 7 60 5 flat 173 4000 3095 4500 3917 11174157 Black grey 37 148 125 100 5 172 4000 3077 4500 3894 174193 Black grey decanting 25 5 172 4000 3077 4500 3894 174158 Black light blue 30 120 3 5 00 6 172 4000 3077 4500 3894 11174224 Blac 19 76 13 5 107 5 7ven 172 4000 3077 4500 3894 210821 Blac 27 108 3 5 07 5 7vac 172 4000 3077 4500 3894 174160 Black red 19 76 6 5 07 7 10vac 172 4000 3077 4500 3894 11174219 o White 16 64 17 120 5 10 flat 180 4000 3220 4500 4075 174208 Black orange 12 48 6 5 25 10 flat 184 4000
42. nt i e fork lift trolley that complies with current regulations and by people having undergone the necessary training Thermo strongly recommends that all operators comply with the local laws and regulations on safety and health in the workplace The machine must be supported from underneath If it has to be transported without its pallet for example on a staircase professional handling assistance is required A e Unpack the centrifuge carefully removing any possible accessories and the material supplied for ordinary operations and maintenance e Check the contents of the package using the packing list provided above e Keep the packaging until the centrifuge has been tested and found fully functional CL40 FL40 3 1 Chapter 3 Installation Positioning Benchtop Units Floor standing units Main Supply 3 2 CL40 FL40 The machine must be installed in a dust and corrosion free room Leave a 30 cm 12 in space free on each side of and behind the machine for safety reasons proper ventilation and maximum cooling performances A Place the centrifuge on a bench top which must be rigid horizontal and sufficiently strong to support the centrifuge s weight and small vibrations Place the centrifuge on a horizontal floor After proper positioning is defined LOCK BOTH WHEELS by pushing with the foot on the wheel locks A Check mains and frequency they must correspond to the values shown on the instrument i
43. ololvelolelolololololololololelelolololololelelalc ca clolololololvelololololol cl oloo XN ol olwlololvwlolvwlolelolelololelelolololelelololelelolelelelvulelvwlolololololvelolololololelolelo XN ol olwlolol lt 2 olvwlolelolololololelolololelelololelelolelelelve elvwlolololololvelolololololelolelo XN ol olwlololelolvwlolelolelololelololololelelololelelolclelalclelvwlolololololvelolololololvelolelo Mn ol O Z ol oj cl o cl ol c olol ol ol ol olol olol al al ol ol al ao ol Z al Z olol Z olol ol olol o ol ol o ol o c O iaj aj ajl Zi c Eel al a Ecl al z a a al a al ww olaja a Lg ZS v al lt cl clala o ol z ol alal ajl a a olal olal gL g zIclolx o ajiz ole Thermo Electron Corporation CL40 FL40 6 7 Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO Chemical o o O jm o jo jo o o O jm o o jo o o O w o o jo Olmo w o o jo olalolal lolo O lu jo jo jo gt a ln nm Z2 2 13 mi E AIM N S IIe D vaju A IE gt gt gt A lA e A S a o gt jo gt gt jojo O o o o lolo Oo o o o jo jo OIDIDOIDIDIO o ol nlolslololo olnlolsljslolo OIDIOIDIDIO o ISO IS Hijo o sS se sS SEA Dole Ie Il 2 HIN se s 2 o e Slelelelelels clio lo O la e ojo o olo lolo o 8 x FPE ppp SE le Sam tele 2 oem 2 2 2 Le Le Le z S Sd 0 0 0 0 0 O 0 0 00 00 0 8 Sd 5 S 0 0 0 0 0 0 0 0 Octane 0 S 0 0 0
44. peed Control M 5 2 Operational Limitations ideal tte us all d i mud 5 2 Aerosol Risks EE 5 2 Service and Maintenance eese cerrar 6 1 Periodic Cleaning ss ii 6 1 Contamination leegent RM adi 6 1 O II E rb S 6 2 Radioactive Decontamination EE 6 2 Power Supply Circuit Broker cit 6 3 Rotor Remolino o 6 3 Trunnion EE 6 3 Chemical Compatibility Table for Rotors Tubes and Accessories 6 4 Disposal of Product 2 1 1 CL40 FL40 ix NOTICE de DEBLOCAGE du SYSTEME de BALOURD Thermo Notice to release the imbalance system ELECTRON COBPCAPON 1 COUPER LA CORDELETTE Cut the string CS es GH c SO cuc E gt SH LD O cc l e Ses A CORDELETTE String 2 RETIRER LA CORDELETTE ET LE CAOUTCHOUC Remove the string and the Rubber ring Pour pr venir les risques d endommagement pendant le transport le syst me de balourd est immobilis en usine A la r ception de l appareil couper et retirer la cordelette fix e sur le panneau arri re To prevent damage during transportation the imbalance system is immobilized in the factory At the receipt cut the string and remove 242709 ind B NOTICE de DEBLOCAGE du SYSTEME de BALOURD Notice to release the imbalance system 1 LIBERER LA CORDELETTE en retirant cette vis de la face avant Remove this scew of front panel and realese the
45. pplied immediately The rotor will be decelerated to rest in few seconds As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides If the message persists despite your efforts to balance the load call your Thermo Service representative CL40 FL40 1 3 Chapter 1 Use and Function 1 4 Relative Centrifugal CL40 FL40 Force Note Imbalance tolerance depends upon the rotor in use The centrifuge will tolerate 15 gr of imbalance with the M rotor equipped with standard buckets Carefully balance the sample load to avoid actuating the imbalance detection system If the protection has not been removed from the imbalance detection device during installation the centrifuge will consider this as an imbalance situation and will not operate Relative Centrifugal Force RCF at the circumference of a rotor and bucket combination is directly proportional to the speed r p m and radius of the rotor Therefore a greater r p m and or a larger radius produces a greater RCF and improved faster separation of substances The centrifuge control system carries out and displays the results of all calculations related to speed radius and RCE Note The value introduced for the radius can be adjusted to allow for position within the tube such as at a boundary Maximum radii are quoted in the specifications tables Use of improper radius will adjust the speed s
46. r agents that have the potential for transmitting disease by infectious aerosols To reduce the risk of inhalation exposure in such an accident Thermo recommends the use of the sealing lids available for the M rotors which are certified for biocontainment through worldwide recognized testing procedures performed in recognized external laboratories 0G2 5 Swing out Roto 062 5 Swing out rotor Cat N 11175382 e 5400 rpm 4140 x g e 2x6 microplates e 2 x column filter blocks Angle Rotors CD 1 angle rotor e 8x100 mL tubes 44 5 mm e min max 106 115 mm 8000 rpm 9588 x g e 35 angle e round bottom Cat No Insert capacity Max tube mm Accessory description 64662300 1 16 5 Set of 8 adaptors for 10 mL vac tubes 3 8 CL40 FL40 Thermo Electron Corporation Chapter 3 Installation CD 2 angle rotor e 10x50 mL tubes 34mm e min max 97 115mm e 8000 rpm 9302 x g e 35 angle round bottom Cat No Insert capacity Max tube mm Accessory description 64726601 1 30 Set of 10 isolators 64724502 1 29 Set of 10 adaptors for 50 mL conical tubes 11174535 1 26 Set of 8 adaptors for 38 mL tubes 11174536 1 24 Set of 10 adaptors for 30 mL Corex tubes 64814700 1 17 5 Set of 10 adaptors for 15 20 mL tubes 64726602 1 16 Set of 10 adaptors for 15 mL vac tubes 11210769 1 Set of 10 adaptors for 15 mL conical CD 3 angle rotor e 40x15mL tubes H 18 5
47. r 1 Use and Function Drain the centrifuge bowl e Insert the plug back into the hose Attention Do not remove the plug from the drain hose during centrifugation A A three phase brushless motor drives the rotating equipment The rotor is contained in a sealed armor plated centrifugation chamber The brushless motor requires no maintenance A control system ensures the correct drive speed which is continuously monitored The CL40 and FL40 centrifuge series are equipped with an interlock system that assures two basic safety features e The run cannot be started if the lid is not correctly closed e The centrifuge lid cannot be opened if the rotor is running The centrifuge will not operate until the lid is closed and latched in place The lid remains latched until the rotor stops spinning The lid access lamp indicates when the rotor has stopped consequently the handle on the right hand side of the centrifuge can be used to open the lid If a power failure occurs access to the samples in the centrifuge is possible via a manual lid opening procedure see appropriate paragraph For this operation a special tool supplied with the centrifuge is required Bypass the interlock system only under emergency conditions as the rotor could still be rotating The CL40 and FL40 centrifuge series are equipped with a load imbalance detector In case of excessive imbalance the load imbalance indicator LED is illuminated and the brake is a
48. rantee is invalid if the unit is incorrectly used poorly serviced or neglected mis used or accidentally damaged There is no explicit guarantee other than as stated above vi CL40 FL40 Thermo Electron Corporation For further information assistance or service United States of America Tel 1 800 522 77 46 Fax 1 866 984 37 66 Canada or Latin America Tel 1 800 522 77 46 Fax 1 866 984 37 66 China Shanghai Tel 86 21 68 65 45 88 Fax 86 21 6445 7907 86 21 6445 7830 India Mumbai Tel 91 22 5542 9494 Fax 91 22 5542 9495 Japan Tel 81 454 53 9220 Fax 81 454 53 9222 Other Asia Pacific Countries Tel 852 28 85 46 13 Austria Tel 43 1 80 14 00 France Tel 33 1 69 18 77 77 33 2 28 03 20 00 Belgium Tel 32 24 82 30 30 Germany Tel 49 61 84 90 69 40 Fax 49 61 03 40 81 012 Finland Tel 35 89 32 91 00 Thermo Electron Corporation Italy Tel 39 02 95 05 91 Netherlands Tel 31 7 65 71 44 40 Russia Cis Tel 7 09 52 25 11 15 Spain and Portugal Tel 34 9 32 23 31 54 Switzerland Tel 41 1 4 54 12 12 United Kingdom and Ireland Tel 44 87 06 09 92 03 Other Europe Middle East or Africa Tel 49 61 8490 6940 Tel 33 2 2803 2000 In Other Countries Contact your local THERMO products distributor or agent E mail Technical Service Representative for products at techsupport led asheville thermo com Visit our web site at www thermo com
49. s 5g D O 2 2 9 1 5 EIDA D oO o gt N IN N N A 2 E E Elo ciciciciczloelo o D ojojjo o jo lt c m a ma a a os jos Jon an 0 S 0 S 0 S 0 0 0 0 0 0000000000 S 0 0 0 0 0 0 0 0 0 S 0000000 0 0 S 0 0 0 0 Butane U 0 MS SMOS S 0 S 0 0 0 0 0 0 0 n Butanol U U S S n Butyl Acetate Butylene U U U 0 0 0 0 U S 0 0 0 0 0 U 0 0 0 U U 0 0 0 0 0 Butyl Chloride Butyric Acid S 0 0 00 0 0 0 U Mt CO OO JOJO Oo o o o o o CO O wo JO Oo o o o o o a lajuna toi O O O O lo a en en en en lala O jm O QOO c o o o z o o 2 o a en en en en lala jo jm o co en en en lnla lajan jalan r valjala lalan en C llo m 1H a co es e loi CO O 2 S jo co CO en 1D en jan jan jm r CO OO JOJO o o o o o o CO O Oo o o o o o o o o CO O Oo o o o o o o o o ololololololololn slo Ioa O 1310 jo jo jo olol nlolololszlolnlolo ololololololololn 5lo gt gt gt gt gt gt D gt D 2 o o 2 2g E 2818 amp Soles SlEloljel2la 2 8 s o S o0lol S lail 2 0 a o ells lelea lE se m O a L I ot SSL lo EE Se pe ae ee ele amp lala sula olololololeolel elelel e GID oo O O lo JO O O O U S SU 00000 U U U 00000000 S
50. string 2 REMONTER et BLOQUER la VIS Rechuck and block the scew 3 RETIRER LA CORDELETTE ET LE CAOUTCHOUC Remove the string and the Rubber ring Pour pr venir les risques d endommagement pendant le transport le syst me de balourd est immobilis en usine A la r ception de l appareil retirer la cordelette fix e entre la grille de protection du moteur et la face avant comme montr ci dessus To prevent damage during transportation the imbalance system is immobilized in the factory At the receipt remove the string placed between the protective grid and the front panel 250841 ind A User Manual Warning Symbols S Of EN A Description Thermo Electron Corporation Chapter 1 Use and Function The user manual is part of the centrifuge and contains important information for your safety and for the best use of the equipment Always keep the manual close to the appliance and in a safe place so that it is always available Thermo strongly recommends that all users read this manual carefully The following symbols are provided to help the operator take advantage of the protection afforded by the equipment and to warn of potential danger On the main switch allows centrifuge to operate A On the main switch disconnects the centrifuge from the main power supply A Only authorized personnel can touch the parts close to this symbol and in any case only after switching off t
51. stros productos Adicionalmente suministramos informacion acerca CL40 FL40 iii WEEE Compliance iv CL40 FL40 France de los productos de Thermo Electron que puedes asistir en la deteccion de substancian mensionades en la directiva RoHS explicado en www thermo com WEEERoHS Conformit WEEE Ce produit doit tre conforme la directive euro p enne 2002 96EC des D chets d Equipements Electriques et Electroniques DEEE Il est merqu par le symbole suivant Thermo Electron s est associ avec une ou plusieurs compagnies de recyclage dans chaque tat membre de l union europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Electron ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Electron qui peuvent aider le d tection des substances sujettes la directive RoHS sont disponibles sur www thermo com WEEERoHS Thermo Electron Corporation Declaration of compliance Declaration de conformite We Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declare that the instrument defined here below complies with the following European directives Declaration of compliance Nous Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France d clarons que le mat riel d sign ci apr s est conforme aux directives europ ennes suivantes Ko
52. the CL40 or FL40 exhausts into the room air which has passed through its bowl Thus the risk of the spread of aerosols is significant in a ventilated centrifuge In the case of samples presenting a biological or chemical risk the operator must take suitable precautions to prevent or reduce this risk CL40 FL40 5 2 Chapter 5 Hazard Precautions and Limitations of Use 5 3 Duration of aerosol presence CL40 FL40 Thermo recommends the use of sealed buckets available for both the M4 rotors which are certified for bio containment through worldwide recognized testing procedures performed in recognized external laboratories A Aerosols are produced naturally when using uncapped tubes or bottles They are also produced when a container deforms or breaks provoking a small or large sample leak We draw your attention to the use of tubes in angle rotors Even if the liquid level does not touch the cap when in a static state it could do so during centrifugation and leak You should therefore follow the instructions of the tube supplier concerning the fill level and sealing of the tube Once a biological risk is known or suspected samples should be placed in a sealed container Should such containers not be available sealed accessories should be used such as angle rotors with sealed lids When aerosols are created in a centrifuge bowl they persist in the air for a period of 3 5 minutes after the rotor stops moving Indeed t
53. tual maximum RCF values whichever is currently selected The lower display shows the given deceleration profile and the LED corresponding to the acceleration button is illuminated At the end of a run after the rotor has been decelerated to rest the centrifuge will produce an audible beep and the middle display will show the word End Pressing any button or opening the lid will bring the displays back to their normal at rest configuration display of set parameters CL40 FL40 4 5 Chapter 4 Instructions for Use Error Codes Error code Origin State of the centrifuge User s action 01 02 03 Incorrect speed Stop without braking Wait for the centrifuge measurement Lid opening impossible till to stop try to launch complete stop the cycle again If the problem remains please contact THERMO service 04 Speed gt 0 RPM at All controls are disabled Wait for the speed to return power ON till the speed returns to to 0 RPM Press any key to 0 RPM exit the alarm mode CLOSE Lid Lid open The centrifuge doesn t start if Close the lid and press the lid isn t properly closed START again 06 Lid open in Stop with braking Wait for the centrifuge to stop running mode close the lid and try to launch the cycle again If the problem remains please contact THERMO service 07 Imbalance Stop with braking Check the correct balancing of he inserts ensure that the runnions are regularly ubricated if
54. uctions L 64227001 Mains cord L 26227005 Toolbox 26339005 e Rotor removal tool L 85241084 e Emergency unlooking tool 1 LI 86000897 e 12 and 13 mm wrench LI 25055501 e 21 mm wrench L For missing parts call your nearest Thermo representative ii CL40 FL40 Thermo Electron Corporation Great Britain Deutschland Thermo Electron Corporation Lx Italia Lx rri o e 9 WEEE Compliance WEEE Compliance This products is required to comply with the European Unions Waste Electrical 82 Electronic Equipment WEEE Directive 2002 96EC It is marked with the following symbol Thermo Electron has contracted with one or more recycling disposal companies in each EU Member State and this products should be disposed of or recycling through them Further information on Thermo Electron s compliance with these Directives the recyclers in your country and information on Thermo Electron products which may assist the detec tion of substances subject to the RoHS Directive are available at www thermo com WEEERoHS WEEE Konformittat Dieses Produkt muss die EU Waste Electrical amp Electronic Equipment WEEE Richtlinie 2002 96ECerf llen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Electron hat Vereinbarungen getroffen mit Verwertungs Entsorgungsanlagen in allen EU Mitgliederstaaten und dieses Produkt muss durch diese Firmen widerverwetet oder entsorgt werden Mehr Informationen ber die Einhaltung dieser An
55. ues press on the corresponding up down arrows Set RCF When the LED next to G 19 is illuminated the upper display shows RCF values To increase decrease the RCF values press on the corresponding up down arrows When using RCF as a control display value make sure the correct centrifugal radius is entered into the control system see below If an incorrect radius value is in the control system the RCF value displayed will not be accurate Set run time When the LED next to TIME 20 is illuminated the middle display shows time values To increase decrease the run time values press on the corresponding up down arrows Set centrifugal radius When the LED next to RADIUS 21 is illuminated the middle display shows the value of the cen trifugal radius in millimeters To increase decrease the centrifugal radius press on the correspon ding up down arrows This parameter can only be viewed and set while the centrifuge is at rest It is essential that this value be correct for the RCF control display function to be accurate The cor rect value of the centrifugal radius depends on the combination of rotor buckets adapters being used Set acceleration profile When the button 8 is pressed the lower display 17 shows the number corresponding to the acceleration profile currently registered 1 to 5 5 being the fastest To change the value press the corresponding up down arrows Set deceleration profile
56. ulation reduces the heat level in the bowl However the temperature in the bowl can reach 10 C above the ambient temperature after hours of continuous operation The CL40R and FL40R are equipped with a powerful refrigeration system that allows samples to be processed at low temperature The refrigerant R134a is free of CFC Chloro Fluoro Carbons in accordance with the Montreal Protocol directions for preservation of the ozone layer in the atmosphere The actual temperature in the bowl is continuously displayed on the front panel of the centrifuge Under certain conditions high speed long duration runs the sample temperature can be higher than the bowl temperature on the display In this case the user may compensate manually after empirical determination of the temperature differential The lid should remain closed when the refrigeration system is in use to avoid condensation i o sr REFER TO INSTRUCTION MANUAL The centrifuge has a rubber hose that allows condensation to be drained from the bowl The drain hose exits at the back of the machine Near the drain hose you can find the following label To remove condensation please follow this procedure Switch off the centrifuge and disconnect the power e Puta small basin under the hose e Unplug the hose Thermo Electron Corporation Drive System Safety Interlock System Imbalance Detection Thermo Electron Corporation System Chapte
57. weisungen durch Thermo Electron die Verwerter und Hinweise die Ihnen n tzlich sein k nnen die Thermo Electron Produkte zu identizfizieren die unter diese RoHS Anweisung fallen finden Sie unter www thermo com WEEERoHS Conformit WEEE Questo prodotto deve rispondere alla direttiva dell Unione Europea 2002 96EC in merito ai Rifiuti degli Apparecchi Elettrici ed Elettronici WEEE E marcato col seguente simbolo Thermo Electron ha stipulato contratti con una o diverse societ di riciclaggio smaltimento in ognuno degli Stati Membri Europei Questo prodotto verr smaltito o riciclato tramite queste medesime Ulteriori informazioni sulla conformit di Thermo Electron con queste Direttive l elenco delle ditte di riciclaggio nel Vostro paese e informa zioni sui prodotti Thermo che possono essere utili alla rilevazione di sostanze soggette alla Direttiva RoHS sono disponibili sul sito www thermo com WEEERoHS WEEE Este producto requiere cumplir con la Directiva 2002 96EC WEEE residuos de quipos electricos y electronicos Este producto esta marcado con el simbolo WEEE como se describe En cada Estado Miembro de la Union Europea EU Thermo Electron ha contratado con diversas companias para disponer y o re ciclar los residuos de equi pos electricos y electronicos Existe information adictional acerca de la conformidad de Thermo Electron con esta Directiva incluyendo los nombres de las diversas companias autorizadas para disponer recyclar nue
58. zed technical service Thermo personnel will not accept to work on instruments deprived of this Decontamination Certificate If an instrument is received at our Service facilities and in our opinion is a radioactive or biological hazard the item will be refused and resent to the Customer Disposition costs will be borne by the sender Instructions for decontamination and cleaning are explained in the User Manual Additional certificates are available from your local technical or Customer Service representative In the event these certificates are not available a written statement certifying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable For your convenience use a copy of this page CL40 FL40 1 2 11290088a

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