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E1 ClipTip User Manual 2015

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1. 71 3 3 2 Single Channel Pipettes Cleaning 71 3 3 3 Single Channel Pipettes Assembly 71 6 3 4 Multi Channel Pipettes 73 3 4 Decontamination Procedure 74 6 5 0 74 Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 3 3 6 Disposal of Materlals 75 3 6 1 Disposal of eMatenals o n unauna aaaea 75 3 6 2 Disposal of the Pipette 76 DI S BIB D oo cue hired barra 76 H TECHNICAL 11 19 J TECHNICAL SERVICE 60 J 1 Packing for 2 90 J2 Contaci uge tte 2 rw 4 os ob Rar cute denne 4eauareetwoededereay 2 0 nad 81 4 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific A INTRODUCTION Congratulations on purchasing a Thermo Scientific E1 GlipTip Electronic Pipette The Thermo Scientific E1 ClipTip is a general purpose SS electronic micropipette that operates on the ai
2. 4670020 j ELCipip2 125u f 4670030 _ E1 ClipTip 10 300 Wl al 4670000 E1 ClipTip 50 1250 ul JMd chmels O 4671000 Eidiptip amp chOsi254 T L L L TL L 467040 trcmpschz z s4 PT T T L TL L 4671070 tiGWprip amp chio300 L L 4671100 ien Steng L L L fe 4671010 ELCiprpizchos1254 L L L L L1 L L or 4671050 ELCipTipizch21284 L L L L TL LT 4671080 E1 ClipTip 12 ch 10 3000 L L EST L LT 4671050 ELCiptipizcn3o8504 L L L L LT 4671020 ELCipTipi amp chOS1254 L L L L L TT T 4671050 ELCiprpiechisou L L TL L PT 4671060 ELCipripi amp h21254 L L L L L ITL L I C Spacing Equalizer aen I LL LL TL T 4672090 1 ClipTip Equalizer 6 ch 50 1250 W1 ej j 14672050 1 Equalizer 8 ch 2 125 T 4 j 4672080 E1 ClipTip Equalizer 8 ch 10 300 W 4 P 4672100 1 Equalizer 8 ch 50 1250 4672010 E1 ClipTip Equalizer 384 8 ch0 5 125ul_ 4672030 1 Equalizer 3848 13 j 4672060 E1 ClipTip Equalizer 3848 ch2 125ul_ P pe 4672020 E1 ClipTip Equalizer 384 12th 05 125 ul pel 4672040 E1 ClipTip Equalizer 384 12 ch 1 30
3. 56 Responsibilities of the User 5 E 4 Performing the Calibration B7 E 4 1 Device Requirements and Test Conditions 5 OCCU Gra e qasaq IEEE RENS TURTERENE ES EE UE DO E 4 3 Calculation Formulas 59 E43 Volume Ce Een uso dco bia hia dete tt pet a ER td c EEA 59 E 4 3 2 IDaCCUraCy 59 Ol eon ortos paquqa yaaa be AR Q chee 60 ED iU I MERERETUR 60 E 5 1 Changing Default Calibration Setting 60 5 2 Creating Special Adjustments 62 E 5 3 Editing Special Adjustments 63 E 5 4 Deleting a Stored Adjustment Setting 63 F TROUBLESHOOTING 64 MAINTENANCE 68 3 1 Regular and Preventive 68 3 2 Replacing Tip Fitting and Sealing 6 69 3 3 Disassembly and Assembly 71 G 3 1 Single Channel Pipettes Disassembly
4. ER 2 Select Date and time and press WV Select Date 3 Scroll in the menu using v 10 21 2013 4 Setthe Date and Time by selecting the corresponding slot and press WE Set 5 Press SQ Done after entering the value 05 56 AM mmvzdd yyyy 6 Date format and Time format can be changed by using gt ai Time format 12h Picture 045 7 Press SQ Done when all the values have been defined Picture D45 Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 49 Calibration tracker Service tracker Product information Date and time Picture D46 D 4 Power Function 50 Thermo Scientific E1 ClipTip User Manual D 3 2 7 Recovery Recovery is used to return the factory settings to the pipette To return the factory settings 1 Select Settings and press WV Select 2 Select Recovery and press 8 Select 3 The pipette warns against clearing all user data Press WP Proceed to continue 4 Press Ok to confirm the recovery process 5 he pipette will restart 6 Scroll in the menu using A v set the Date Time and the desired Date and Time formats by selecting the corresponding slot and press WV Set 7 Press W Done after entering the value 8 Press SQ Done when all the values have been defined The Recovery function can be protected with a password Picture D46 See section D 3 1 3 Caution All user edited programs calibrati
5. Use Select the function to use Edit Press Edit Use the Left Right keys to select Volume based dilution Use the Up Down keys to highlight Fill 1 volume Use the Left Right keys or the Volume editor to adjust the volume to 90 pl Use the Up Down keys to highlight Fill 2 volume Use the Left Right keys or the Volume editor to adjust the volume to 10 pl cO Done The function is ready to run per oad tr Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 53 Using Matrix Select Select the Matrix icon from the main menu Matrix Select Fill step is added mM Use the Left Right keys or the Volume editor to adjust the volume to 90 pl Done he volume is accepted Add Add a new step 4442444249442 4 Use the Down key to highlight Air gap Select Air step is added Use the Left Right keys or the Volume editor to adjust the volume of 20 pl Done The volume is accepted Add Add a new step Select Fill step is added Use the Left Right keys or the Volume editor to adjust the volume to 10 pl Done The volume is accepted Done The editing is ready Ok b d Purge step is added Done The function is ready to run Timed Dispensing Example Using the timer in initiation of enzymatic reactions at 30 s intervals a 1 Note he timer is available in Matrix only 54 Th
6. Warning An old battery can cause the pipette to malfunction and may cause a safety risk thermal runaway We recommend that you change the battery every two years If the battery s recharging interval decreases significantly the battery should also be changed It is recommended to charge the pipette every two months even if the pipette is not used daily Warning The E1 ClipTip pipette contains a Li ion battery and a potential malfunction of the battery short circuit mechanical damage overheating etc may cause explosion Warning Do not use a battery that causes the following notifications battery failure 2 or 3 on the pipette display Such a battery may cause a potential safety risk For more instructions see Chapter F TROUBLESHOOTING Thermo Scientific E1 ClipTip User Manual 9 gt PD D A 3 Getting Started gt 10 Thermo Scientific E1 ClipTip User Manual Caution Use only the power transformer supplied by Thermo Fisher Scientific Use of an incompatible power transformer can damage the pipette Caution Always turn off the power and unplug the power transformer before cleaning the exterior Fluid seepage can damage internal components Caution Do not autoclave the entire unit Extreme heat can damage the display and other electronic components Caution Do not clean the keypad with bleach solution or other solvents Caustic cleaning solutions can damage the keypad Note Do not hold the pipette horizontal
7. 34 0 2 2 4 34 O SDI CES 39 DZA PORNO e on on ee EE d nus sua PEPPER a oq kau RETE TUE se 37 MIX EE EE a EE EES E 38 H e NEE 40 023 FTOgrats Egeter ades ce aii piri ote apuk tte rnt yi eie 41 2 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific D3 oupportnd FUFCHOfIS L 23 mcm dr xr nin th mur d adc e d ait 43 DS l IS EE 43 PARET DEE A Ee TOT TTE TETTE OEIL 43 o2 0 On D 44 0 3 1 3 Organize 5 45 03 14 Password HOC EE 46 EEN e E Eh SN KEE E EE 47 EE 47 VISAS I UU REESE 47 3 2 0 Calibration Mackel s crni usss buddy 47 D 3 2 4 Service 48 D3 u u cert ent qu yal L vut erac 49 0 3 2 6 Date and 49 REVENU 50 3 2 9 Gonnet PISTON a kasa 50 D 4 Power FunCtlOn 50 D 5 Application Examples 51 E CALIBRATION AND ADJUSTMENT 56 E 1 lermino0 ogy 56 E 2 Factory Calibration
8. Service tool A 1062800 Service tool B 1062500 not included in multichannel pipettes Tip Fitting tool C 2215870 not included in 384 multichannel pipettes Instructions for Use Manual Tip Fitting sealing ring not included in 384 pipettes ClipTips sample Li ion battery LO N O N Remove the content from the package and check that all items listed above are Included Inspect for possible shipping damage Make sure that the pipette has the desired volume range and that the voltage of the charger IS correct A 1 Thermo Scientific E1 ClipTip Pipette Models single Channel Multichannel Equalizer Adjustable Tip spacing Multichannel 6 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific A 1 1 Thermo Scientific E1 ClipTip Model Selection E1 ClipTip Single Channel models Cat No 4670000 4670020 4670030 4670040 Channels A k P gt E1 ClipTip Multichannel models Cat No 4671000 4671040 4671070 4671100 4671010 4671050 4671080 4671090 4671020 4671030 4671060 Channels Format 96 96 96 96 96 96 96 96 384 384 384 Volume Range ul 0 5 12 5 2 125 10 300 50 1250 Volume Range ul 0 5 12 5 2 125 10 300 50 1250 0 5 12 5 2 125 10 300 30 850 0 5 12 5 1 30 2 125 Calibration Range pl 1 25 12 5 12 5 125 30 300 125 1250 Calibration Range ul 1 25 12 5 12 5 125 30 300 125 1250 1 25 12 5 12 5 125 3
9. 10 Speed out 10 Counter Picture D8 Thermo Fisher Scientific 1 The volume can be changed by pressing Edit or A while in the active pipetting function Picture D4 2 The Volume field will be highlighted Picture D5 3 Press to activate the Volume editor Picture 06 4 When the last number of the volume is highlighted Picture D6 use 9 to move into the volume list The volume at the top of the list will be automatically highlighted Picture D7 Use A to navigate in the volume list 5 When the preferred volume is highlighted press SQ Done to enter into the basic volume field to continue editing D 1 3 Pipetting Speed Adjustment It is possible to adjust aspiration speed in and dispensing speed out separately in the E1 ClipTip pipette There are 10 different speeds available speed can be set as a value from 1 slowest to 10 fastest The speed is also indicated as a horizontally laid set of bars located next to the speed value The number of bars is the same as the maximum speed value Dark bars starting from the left edge indicate the current value The other bars have a lighter color 1 Press 8 Edit or A v in the pipetting mode view Picture D4 2 Use M W to highlight the Speed in or Speed out Picture 09 3 Use a to increase the speed and 2 to decrease the speed 4 Press Done when the changes are ready Thermo Scientific E1 Cl
10. 30 00 15 00 3 00 125 00 62 50 12 50 Inaccuracy ul 3 20 256 3 20 395 12 3 20 27550 8 0 2 6 8 0 239 8 0 26 67 32 00 756 32 00 5 12 32 00 425 60 32 00 2 56 32 00 15 12 3200 7550 0 400 3 20 0 400 6 40 0 400 32 00 1 00 333 1 00 6 67 41 00 33 33 3 20 2 56 3 20 5117 3 20 2550 0 400 3 20 0 400 6 40 0 400 32 00 1 00 333 1 00 667 230 36 3 20 256 3 20 5 12 3 20 27550 Imprecision std ul CV 1 20 0 96 1 20 1 82 1 20 9 60 3 0 100 3 0 2 00 3 0 1000 12 0 0 96 120 192 12 0 9 60 12 0 0 96 120 1 92 12 0 9 60 0 200 1 60 0 200 3 20 0 200 16 00 0 40 1 33 040 267 0 40 13 33 1 20 0 96 120 1 92 1 20 9 60 0 200 1 60 0 200 3 20 0 200 16 00 0 40 1 33 0 40 267 0 40 13 33 1 20 0 96 1 20 1 92 1 20 9 60 ClipTip 1000 ClipTip 12 5 384 ClipTip 30 384 ClipTip 200 ClipTip ClipTip 200 ClipTip 300 ClipTip 1250 ClipTip 1250 ClipTip 12 5 394 ClipTip 30 384 ClipTip 125 304 ClipTip 12 5 304 ClipTip 30 384 ClipTip 125 304 Thermo Fisher Scientific Appendix 4 Assembly and Spare Parts Avallable as a spare part See model specific order numbers 1062800 1062500 2215870 Tip fitting tool Service tool Service tool 12905600 Charger Adapter EUR 5 Tip fitting 42905590 Battery Lithium lon E1 Multichannel pipettes E y FE T bi 6 es 96 format models 125
11. 46720707 E1 ClipTip Equalizer 384 12 ch 2 154 J e Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 93 Appendix 6 GlipTip Ordering Information ClipTip Racked non sterile and sterile Order number 94410040 94410043 94410210 94410213 94410250 94410253 94410310 94410313 94410510 94410513 94410710 94410713 94410810 94410813 ClipTip 384 tip 94410050 94410053 94410100 94410103 94410150 94410153 Description ClipTip 12 5 ClipTip 12 5 sterile ClipTip 20 ClipTip 20 sterile ClipTip 50 ClipTip 50 sterile ClipTip 200 ClipTip 200 sterile ClipTip 300 ClipTip 300 sterile ClipTip 1000 ClipTip 1000 sterile ClipTip 1250 ClipTip 1250 ClipTip 384 12 5 ClipTip 384 12 5 sterile ClipTip 384 30 ClipTip 384 30 sterile ClipTip 384 125 ClipTip 384 125 sterile Volume range 0 5 12 5 ul 0 5 12 5 ul 1 20 ul 1 20 ul 5 50 ul 5 50 ul 5 200 ul 5 200 ul 10 300 ul 10 300 ul 30 1000 ul 30 1000 ul 50 1250 ul 50 1250 ul 0 5 12 5 ul 0 5 12 5 ul 1 30 ul 1 30 ul 2 125 ul 2 125 ul Color Coding Pink Pink Pink Pink Violet Violet Yellow Yellow Orange Orange Blue Blue Turquoise Turquoise Pink Pink Violet Violet Yellow Yellow Oty 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 8 x 96 rack 8 x 96 rack 8 x 96 rack 8 x 96 rack 10 x 384 rack 10
12. Caution The pipette must be charged at temperatures between 0 to 45 he battery may be damaged if charged at other temperatures For indoor use only Warning Use of incorrect power supply units may result in fatal injuries and damage to the device gt P SCIENTIFIG Cat no 12905600 Use of incorrect power supply units may result in overheating burning melting short circuiting of the E1 ClipTip pipette or similar damage Only use the supplied power supply unit for charging the pipette You can recognize the correct power supply by the Thermo Scientific logo and the pipette name on the power supply unit Do not charge the pipette in a hot location gt 45 C A 3 4 Using the Charging Stand Connect the lead of the charger to the socket on the back of the charging stand Then connect the charger to an AC wall socket Place the pipette in to the stand as shown in the picture See also the previous section A 3 3 Using the Power Supply 12 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific A 3 5 Turning the Pipette On and 0ff After the battery Is charged the device can be powered up 1 Press one of the selection keys 7 WP or arrow keys A v gt a below the display for more than 1 s Note that tip ejection buttons not available See section B 7 Keypad and Operation Keys for more information about the keypad 2 If the battery was Just installed then you need to set the date and t
13. Thermo Scientific E1 ClipTip Electronic Pipette Single Channel Multichannel Adjustable Tip Spacing Equalizer User Manual CONTENT A INTRODUCTION 5 A 1 Thermo Scientific E1 ClipTip Pipette Models 6 A 1 1 Thermo Scientific E1 ClipTip Model Selection 7 25 22222222 2 22 9 A 2 1 Intended 1 2 2 2 9 A 2 2 Safety Symbols and Markings 8 A 2 3 Safety Precautions 9 A 3 Getting 10 Pooch 10 A 3 2 Charging the 11 X 3 3 Using the Power SUDOIW Bolt dr iS c dedo 11 A 3 4 Using the Charging Stand 12 A 3 5 Turning the Pipette On and 13 TOW SAVING u l ser ea da daret qa 13 B PIPETTE OVERVIEW 14 B T COMPONGUES 222 222 sss u p a RUE Q aaah OR haqq Wa 14 B 2 Keypad and Ope
14. 1 86 0 60 0 60 1 50 Clip Tip 1250 96 format 8 ch 4672010 79 2 79 1 98 0 60 0 60 1 50 Clip Tip 1250 384 format 8 ch 0 5 12 5 4672030 4672060 384 format 8 ch 1 0 30 0 0 200 0 150 0 150 0 2 0 30 0 21 1 60 2 40 12 00 0 90 2 00 7 00 ClipTip 12 5 304 ClipTip 30 384 384 format 8 ch 2 0 125 0 4672020 4672040 384 format 12 0 5 12 5 0 75 0 80 0 50 0 200 0 150 0 150 0 60 1 28 4 00 1 60 2 40 12 00 0 27 0 90 ClipTip 125 304 ClipTip 12 5 304 304 format 12 ch 1 0 30 0 4672070 384 format 12 ch 2 0 125 0 Range Volume Inaccuracy ul ul 2 0 125 0 12500 750 700 62 50 1 25 200 12 50 1 00 8 00 10 0 300 0 30000 60 2 00 150 00 30 2 00 30 00 1 5 5 00 50 0 1250 0 1250 00 1800 1 44 625 00 9375 150 125 00 50 4 00 50 0 1250 0 1250 00 18600 1 44 625 00 9375 11 50 125 00 50 4 00 12 50 40 313 2 50 6 25 0 156 2 50 1 25 0150 12 00 30 00 0 60 7200 15 00 0 30 7200 3 00 0 30 1000 125 00 250 2700 62 50 1 25 200 12 90 1 00 8 00 12 50 40 313 2 50 6 25 40156 2 50 1 25 0150 12 00 30 00 0 60 7200 15 00 0 30 200 3 00 0 30 1000 125 00 250 700 62 50 1 25 200 12 90 8 00 0 30 0 21 0 75 0 80 2 00 7 00 0 60 1 28 ClipTip 30 384 ClipTip 125 384 1 00 86 Thermo Scientific E1 ClipTip User Manual 0 50 4 00 Thermo Fisher Scientific Appendix 3 IS08655 Calibration Specificati
15. Scroll to Speed and change the value using D 9 Press Done when the changes ready Purge emptying the tip Change the speed using gt 2 Press SQ Done when the speed has been changed Mix mixing by pipetting a selected volume select the mix type User controlled or Cycle based using gt a scroll to Mix volume and change the value using 9 ocroll to Speed and change the value using 4 If Cycle based is enabled change the amount of mix cycles using D 4 Scroll to Mix counter and select Counter off Counter on using L l 2 Press QQ Done when the changes ready Air gap a selected volume of air used to separate two liquids Change the volume using ei J or the Volume editor section D 1 2 Scroll to Speed and change the value using gt d Press Done when the changes are ready Auto repeated dispensing of the same volume on defined time intervals Change the volume using gt 4 or the Volume editor section D 1 2 ocroll to Repetitions and change the number using L d ocroll to Speed and change the value using gt d scroll to Time and change the value using d Press QQ Done when the changes are ready 30 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific Thermo Fisher Scientific Timer selected time before the next step can be performed he time is defined showing two numbers for the minutes and two n
16. 0048 1 0048 28 5 1 0047 1 0048 1 0048 1 0049 1 0049 1 0050 1 0050 29 0 1 0049 1 0049 1 0050 1 0050 1 0051 1 0051 1 0051 23 9 1 0050 1 0051 1 0051 1 0052 1 0052 1 0052 1 0053 30 0 1 0052 1 0052 1 0053 1 0053 1 0054 1 0054 1 0054 84 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific Appendix 2 Manufacturer Factory Specification Limits E1 ClipTip Single Channel models Thermo Fisher Scientific Cat No Range Volume Inaccuracy Imprecision ClipTip ul ul std ul CV 4670000 0 5 12 5 12 50 40 125 3100 0 050 0 40 Cliplip 12 5 6 25 0 063 1 00 0 050 0 80 1 25 0 050 400 0 050 4 00 4670020 2 0 125 0 125 00 0 75 0 60 0 25 0 20 ClipTip 200 62 50 0 375 060 0 125 0 20 12 50 0 30 2 40 0 10 0 80 4670030 10 0 300 0 300 00 1 8 0 60 0 45 0 15 ClipTip 300 150 00 0 9 0 60 0225 0 15 30 00 0 5 1 67 0 15 0 50 4670040 50 0 1250 0 1250 00 6 0 0 48 1 625 0 13 ClipTip 1250 625 00 43 125 2050 0 813 0 13 125 00 3 0 2 40 0 6 0 48 E1 ClipTip Multichannel models Cat Channels Range Volume Inaccuracy Imprecision ClipTip ul std ul CV 4671000 8 ch 0 5 12 5 12 50 0 313 250 0 200 1 60 Cliplip 12 5 6 25 0 156 250 0 150 740 1 25 0 150 1200 0 150 12 00 4671040 8 ch 2 0 125 0 125 00 2 50 2 00 075 0 60 Cliplip 200 62 50 1 25 2 00 0 80 1 28 12 50 1 00 8 00 050 4 00 4671070 8 ch 10 0 300 0 300 00 6 0 2 00 1 8 0 60 Cliplip 300 150 00 3 0 2 00 1 2 0 80 30 00 1 5 500 0 6 2 00 4671100 8 ch 50 0 1250 0
17. Picture D24 Dilute Mix Purge Picture D25 38 Thermo Scientific E1 ClipTip User Manual D 2 2 7 Dilute Mix With the Dilute Mix mode it Is possible to dispense two selected volumes with an air gap in between followed by mixing of the liquid To use the mode 1 Select Presets and press WV Select 2 Select Dilute Mix from the list and press 88 Use 3 Place the tips under the surface of liquid 1 and press the trigger to aspirate the liquid fill 1 4 Press the trigger to aspirate an air gap 5 Place the tips under the surface of liquid 2 and press the trigger to aspirate the liquid fill 2 6 Press the trigger to dispense the total volume Picture D24 7 Place the tips under the surface of the liquid Press the trigger to start mixing 8 a User controlled mix type As long as the trigger is pressed down ca 70 of the total volume is pipetted 8 b Cycle based mix type The set amount of mixing cycles is done by the pipette If the mix counter is set on a number indicating the amount of mixing cycles appears in the bottom left corner of the display Picture D25 9 Next the display shows Purge Press the trigger to empty the tip purge To change the settings volumes used in the Dilute Mix mode can be set in two different ways ratio based and volume based he ratio based method uses different dilution ratios to determine the volumes to be used Total volume an
18. To change the settings The volume speeds and counter options can be edited Picture D15 1 Press 89 Edit in the pipetting mode view 2 Change the volume using a or the Volume editor section D 1 2 3 Scroll to Speed in and change the value using gt a 4 Scroll to Speed out and change the value using gt a 5 ocroll to Counter and select Counter off on using 9 D Press SQ Done when the changes are ready D 2 2 2 Stepper Multi dispensing Repeated dispensing of a selected volume is possible with the stepper mode The stepper mode is particularly suitable for microplate applications To use the mode 1 Select Presets and press Select 2 Select Stepper from the list and press Use 3 Place the tip s under the liquid surface and press the trigger to aspirate the liquid 4 Press the trigger to discard the pre step if in use 5 Press the trigger to dispense the first step and repeat until all steps have been dispensed 6 Press the trigger to empty the tip purge To change the settings 1 Press SQ Edit in the pipetting mode view 2 Change the volume using J or the Volume editor section D 1 2 3 Scroll to Repetitions and change the amount using 4 4 Scrollto Speed and change the value using 2 a 5 Scroll to Speed out and change the value using gt d 6 Pre step is an additional dispensing step that may improve the results when using certain liquids The defaul
19. field of the display when the set time limit has been reached Also the counter from the last service can be viewed in this mode 1 Select Settings and press 88 Select 2 Select Service tracker and press WV Select 3 Move in the menu using A v 4 Switch the tracker on off using 5 9 5 Scroll to Reminder interval Using gt a the Tracker interval set window will open Set the interval using GY anc 5 4 he interval can be set between 1 to 999 days 6 Press SQ Done after setting the interval Thermo Fisher Scientific 7 The tracker counter can be reset by pressing WV Reset tracker 8 Exit the Service tracker mode by pressing WP Done 9 Press Yes to verify the changes e Note he tracker counter is not reset automatically The user only needs to perform the reset manually see Step 7 D 3 2 5 Product Information 1 Select Settings and press WV Select 2 Select Product information and press Select 3 he product information will appear on the screen This is important In product specific customer enquiries and product service issues Provide this information when corresponding with the manufacturer or dedicated service houses D 3 2 6 Date and Time he Date and time option allows the user to change the corresponding settings The date and time affect calibration and service tracker functions To edit the date and time settings 1 Select Settings and press 88 Select
20. icon shortcut slots available in the E1 ClipTip pipette Shortcuts are placed in the main menu in addition to the six main function icons shortcuts can be created for Presets pipetting modes or user created programs saved under the Programs function The E Icon is used for Presets pipetting mode shortcuts and the Icon for program shortcuts The name of the pipetting mode or program will be displayed below the icon Thermo Fisher Scientific shortcuts Shortcut 2 Shortcut 3 Mone Shortcut 4 Picture D37 7 ga Organize Picture D38 Thermo Fisher Scientific Creating a shortcut 1 Select My Pipette and press 7 Select 2 Select Shortcuts and press WV Select 3 A list of four icon shortcut slots is displayed Picture 036 4 Use M W to highlight the preferred slot D Press 7 Change to change the content of each shortcut 6 Alist of Matrix and Presets functions pipetting modes and stored programs will appear 7 Use A v to highlight the preferred mode or program or None and press Ok 8 Select the icon color using D a press WV Ok 9 Press SQ Done when the changes are ready 10 created shortcut appears at the top of the main menu view Picture D37 Note When a pipetting function or program is edited through a shortcut the changes will also be stored in the original function or program When the original function or program is edit
21. kPa 80 85 90 95 100 101 3 105 15 0 1 0017 1 0018 1 0019 1 0019 1 0020 1 0020 1 0020 15 5 1 0018 1 0019 1 0019 1 0020 1 0020 1 0020 1 0021 16 0 1 0019 1 0020 1 0020 1 0021 1 0021 1 0021 1 0022 16 5 1 0020 1 0020 1 0021 1 0021 1 0022 1 0022 1 0022 17 0 1 0021 1 0021 1 0022 1 0022 1 0023 1 0023 1 0023 Pes 1 0022 1 0022 1 0023 1 0023 1 0024 1 0024 1 0024 18 0 1 0022 1 0023 1 0023 1 0024 1 0025 1 0025 1 0025 18 5 1 0023 1 0024 1 0024 1 0025 1 0025 1 0026 1 0026 19 0 1 0024 1 0025 1 0025 1 0026 1 0026 1 0027 1 0027 195 1 0025 1 0026 1 0026 1 0027 1 0027 1 0028 1 0028 20 0 1 0026 1 0027 1 0027 1 0028 1 0028 1 0029 1 0029 20 9 1 0027 1 0028 1 0028 1 0029 1 0029 1 0030 1 0030 21 0 1 0028 1 0029 1 0029 1 0030 1 0031 1 0031 1 0031 21 9 1 0030 1 0030 1 0031 1 0031 1 0032 1 0032 1 0032 22 0 1 0031 1 0031 1 0032 1 0032 1 0033 1 0033 1 0033 22 5 1 0032 1 0032 1 0033 1 0033 1 0034 1 0034 1 0034 23 0 1 0033 1 0033 1 0034 1 0034 1 0035 1 0035 1 0036 239 1 0034 1 0035 1 0035 1 0036 1 0036 1 0036 1 0037 24 0 1 0035 1 0036 1 0036 1 0037 1 0037 1 0038 1 0038 24 5 1 0037 1 0037 1 0038 1 0038 1 0039 1 0039 1 0039 25 0 1 0038 1 0038 1 0039 1 0039 1 0040 1 0040 1 0040 29 5 1 0039 1 0040 1 0040 1 0041 1 0041 1 0041 1 0042 26 0 1 0040 1 0041 1 0041 1 0042 1 0042 1 0043 1 0043 26 5 1 0042 1 0042 1 0043 1 0043 1 0044 1 0044 1 0044 21 0 1 0043 1 0044 1 0044 1 0045 1 0045 1 0045 1 0046 215 1 0045 1 0045 1 0046 1 0046 1 0047 1 0047 1 0047 28 0 1 0046 1 0046 1 0047 1 0047 1 0048 1
22. motion stops when the trigger Is released 6 When the trigger has been released before achieving the Limit volume the movement direction be changed by pressing SQ Out or WP In the text guided button depends on the current movement direction Picture D27 7 Dispensing the liquid will show as a descending value on the display 8 Optional The volume value on the display can be reset to 0 at any point by pressing WV Reset Cancel The Reset Cancel selection window appears Picture D28 Select Reset using eh a and press 7 Done he volume on the display is 0 Dispensing liquid from the tip will show as a negative value 9 Optional The ongoing dispensing sequence can be interrupted by pressing WE Reset Cancel Reset Cancel selection window appears Select Cancel using gt a and press 7 Done Press W Yes to cancel the dispensing sequence Purge will appear on the display Press the trigger to dispense Thermo Fisher Scientific Manual Volume editor Picture D29 Programs Picture 030 Thermo Fisher Scientific Note After all the liquid has been dispensed the Back button will appear again Press SQ Back to go back to the Presets list To change the settings 1 Press 7 Edit in the pipetting mode view 2 Change the limit volume using gt 9 or the Volume editor section D 1 2 The limit volume value is the maximum volume that can be aspirated The limi
23. pipette features a simple pipetting view including all the information needed during the pipetting cycle The pipetting view can be entered via the Matrix Presets and Programs icons typical pipetting view layout is explained below A Pipetting function D Battery indicator E Pipetting speed in out with indicator B Ongoing pipetting step and volume C Upcoming pipetting step gt Dispense 12 50 followed after ongoing step E F Text guidance Picture D3 Display layout and functionality A Name of the ongoing pipetting function B he ongoing pipetting step and the volume During aspiration the arrow points upwards and downwards during dispensing Different arrow types and colors are used to show different functions such as purge and mixing C Indicates the following pipetting step taking place after the ongoing step D Battery indicator E Pipetting speed indicator he number next to the arrow indicates the present speed setting The arrow changes direction depending on whether the pipette Is aspirating arrow up or dispensing arrow down F Text guidance for both selection keys Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 25 Forward D 0 1 2 Volume Adjustment gt Dispense 300 0pl Back Picture D4 Forward Speed out 10 Counter Counter off Volume editor Done Picture D5 Volume Picture D6 26 Thermo Scientific E1 ClipTip
24. series of 5 doses at both points With multichannel pipettes all channels are calibrated Calibration is performed in a monitored environment Note Calibration results are place and environmental condition specific Factory calibration assures that the devices were functional on leaving the factory he user is responsible for checking that the calibration applies to in the intended use Thermo Fisher Scientific E 3 Responsibilities of the User E 4 Performing the Calibration To assure the proper functionality of the device the user is responsible to e Follow the instructions of this Instructions for Use booklet e Assure that the device and used accessories are suitable for the intended application and are functioning properly in the intended conditions e Define a regular service interval for the device calibration check and maintenance depending on the conditions of use The starting recommendation 15 every 3 months but this can be changed according 10 the follow up results Devices should be serviced at least once a year e Define suitable acceptance criteria for calibration As many premises do not necessarily meet the strict environmental and device requirements stated in the ISO 8655 standard the user should establish acceptance criteria that meet the demands of the tasks that the devices are used for he following calibration procedure is recommended to be performed under strict conditions defined below It is recogniz
25. the actual volume to correspond to the target volume Default calibration Adjustment setting for Matrix and Presets functions he pipette uses this parameter Special adjustment A user created parameter setting that can be stored in the pipette to be used in the Programs function to improve the device performance Manufacturer specifications Acceptance criteria for pipettes used by the manufacturer under strictly defined conditions Used for quality control purposes 150 8655 specifications Acceptance criteria for pipettes recommended for pipette service or end users under strictly defined conditions Allows more tolerance for different influencing factors Inaccuracy accuracy he error of the measured mean volume to the target volume Can be shown as an absolute value e g A 1 0 ul or as a relative value eg ACC 0 1596 Inaccuracy is a systematic error Imprecision precision Statistical evaluation value of the calibration measurement series Can be shown as an absolute standard deviation value e g s 1 0 ul or as a relative coefficient of variation value e g CV 0 1 Imprecision is a random error Nominal volume maximum volume indicated by the device volume range All Thermo Scientific pipettes are factory calibrated and adjusted to give the volumes as specified with distilled or deionized water he performance values are defined at the nominal maximum volume and at 1096 of the nominal volume using
26. the step based operating technique Each pipetting UM step Is entered separately in the sequence in which they will be performed E1 ClipTip Programs My Pipette Presets includes application based pipetting styles The pipetting sequence Is preset Only volumes and speeds have to be set Settings Power Storing different kinds of programs can be done under the Programs icon Picture D1 Twenty programs with specific names and calibrations can be stored Support functions are divided into three icons My Pipette Settings and Power Navigate to the desired icon in the main menu by using the A v and gt 4 keys Press 99 Select to select the icon of interest Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 23 NM w E A I Z Matrix Presets Programs Settings Power add and save programs select function Fill Dispense y Multi Purge Mix Air Note text Tip ejection Forward Stepper Reverse il Repetitive Dilute Mix Picture D2 Menu Map 24 Thermo Scientific E1 ClipTip User Manual Forward stepper Repetitive Forward Mix Dilute Mix Sounds Service tracker E Product information Date and time Recovery single channel models only Thermo Fisher Scientific D 1 1 Introduction to Pipetting View The E1 ClipTip
27. the technical customer support USA Email ushud technicalservices thermo com Europe and APAC Email info pipettes thermofisher com Major service locations and countries Australia Thermo Fisher Scientific Attn Pipette Service Lab 4 Caribbean Drive Scoresby Victoria 3179 Phone 1300 735 292 Email serviceAU thermofisher com Finland Thermo Fisher Scientific Unity Lab Services Attn Pipette Service Rahtikatu 2 80100 Joensuu Phone 358 9 329100 Accredited pipette calibration available FINAS France Thermo Fisher Scientific Unity Lab Services Attn Pipette Service Lab Bd Sebastien Brant BP 50111 F 67403 Illkirch Cedex Phone 33 3 88 67 53 30 Email unity fr thermofisher com Accredited pipette calibration available COFRAC Thermo Scientific E1 ClipTip User Manual 81 82 Thermo Scientific E1 ClipTip User Manual Germany Thermo Fisher Scientific Unity Lab Services Attn Pipette Service Lab Robert Bosch Strasse 1 D 63505 Langenselbold Phone 49 6184 906000 Email unity de thermofisher com Accredited pipette calibration available DKD Japan Thermo Fisher Scientific Attn Pipette Service Lab C 2F 3 9 Moriya Cho Kanagawa ku Yokohama shi Kanagawa ken 221 0022 Phone 81 45 453 9227 Email info pipettes p2thermofisher com Accredited pipette calibration available JAB Netherlands Thermo Fisher Scientific Unity Lab Services Industrieweg 13 4051 BW Och
28. ul and 300 ul 5 11072080 Tip fitting 300 6 2214920 Tip fitting sealing ring 300 12 pcs 7 1030060 Sealing ring small 300 96 format models 850 ul and 1250 ul 5 11072240 Tip fitting 1250 6 2214945 Tip fitting sealing ring 1250 12 pcs 7 1030060 Sealing ring small 1000 Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 89 Single channel pipettes 0 5 12 5 ul 0 5 12 5 ul 5 1064960 7 1030060 15 1030170 16 1030060 42 2215610 5 125 u 5 11072080 6 22149303 pcs 7 1030060 15 1033380 42 2215590 42 assembly 90 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific 50 1250 yl 10 300 ul 11072080 2214930 3 pcs 7 1030060 15 1033180 42 2215580 90 1250 ul 11072200 2215540 3 pcs 1033410 1033420 2215570 o m P p un Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 91 Appendix 5 ClipTip System Compatibility Chart Tp Si 2 00 ui i gt ala a Q Q Q Q Q Q Q Q 5 5 5 5 5 Order No Description O o o o o maman EE oem sums pra 1 4641180 F1 Oe 2 4641190 Faes T 4641200 12 0 0 S 4641210 F1 ClipTip 20200 4641220 F1 ClipTip30 300u 4641230 F1 ClipTip 100 1000 J P
29. x Z 5 Thermo Scientific E1 ClipTip User Manual 59 E 5 Adjustment EE 60 Thermo Scientific E1 ClipTip User Manual E 4 3 3 Imprecision n i s standard deviation ul y average volume n number of measurements Vi individual measurement result 1 1 n CV 100 x V In the E1 ClipTip pipette there are two different ways to affect the adjustment settings The user can change the settings for Default calibration which affects all modes under the Presets menu Or the user may create his her own special adjustments and store them in the pipette to be used in the Programs mode for created dispensing programs Perform the calibration using the protocols of the desired application section D 4 before starting the adjustment procedures Adjustment can be made for example for liquids of different temperature or viscosity or for different pipetting methods such as reverse or multi dispensing In the E1 ClipTip calibration the max volume always corresponds to the nominal volume of the pipette while the min volume always corresponds to 10 of the nominal volume Note According to the 1508655 the acceptance specifications mentioned in this document apply only in the Forward mode Typical performance values for the stepper mode can be inquired from the manufacturer Note he user may adjust the device for the stepper mode by defining the one point calibration in the Programs mode See sections D 2 3 E 5 2 and
30. 0 3000 300 00 8 0 267 3 0 1 00 ClipTip 300 150 00 8 0 5 99 40 2 00 30 00 8 0 26 67 3 0 10 00 Thermo Scientific E1 ClipTip User Manual 87 4671090 4671020 4671030 4671060 12 ch 16 ch 16 ch 16 ch 152 1229 1 0 30 0 240 1250 30 0 850 0 850 00 425 00 39 00 12 50 6 29 1 259 30 00 15 00 3 00 125 00 62 50 12 50 16 00 16 00 16 00 0 400 0 400 0 400 1 00 1 00 1 00 3 20 3 20 3 20 1 88 3 6 18 82 3 20 6 40 32 00 3 33 6 67 33 33 2 56 19 17 25 60 6 0 6 0 6 0 0 200 0 200 0 200 0 40 0 40 0 40 1 20 1 20 1 20 0 71 1 41 7 06 1 60 3 20 16 00 1 33 2 67 13 33 0 96 1 92 9 60 E1 ClipTip Adjustable Tip Spacing Equalizer Multichannel models Cat No 4672050 4672080 4672090 4672100 4672010 4672030 4672060 4672020 4672040 4672070 Channels 96 format 8 ch 96 format 8 ch 96 format 6 ch 96 format 8 ch 384 format 8 ch Range Z 0 129 0 10 0 300 0 90 0 1250 0 90 0 1250 0 j KE 384 format 8 ch 1 0 30 0 384 format 8 ch 2 0 125 0 384 format 12 ch 0 5 12 5 384 format 12 ch 1 0 30 0 384 format 12 ch 2 0 125 0 88 Thermo Scientific E1 ClipTip User Manual Volume ul 125 00 62 50 12 50 300 00 150 00 30 00 1250 00 625 00 125 00 1250 00 625 00 125 00 12 50 6 25 1 29 30 00 15 00 3 00 125 00 62 50 12 50 12 50 6 25 1 25
31. 0 300 85 850 1 25 12 5 3 30 12 5123 E1 ClipTip Adjustable Tip Spacing Equalizer Multichannel models Cat No 4672050 4672080 4672090 4672100 4672010 4672030 4672060 4672020 4672040 4672070 Calibration Range according to 1508655 standards Thermo Fisher Scientific Channels mm 9 14 2 9 14 2 9 19 8 9 14 2 4 5 14 2 4 5 14 2 4 5 14 2 4 5 9 4 5 9 4 5 9 Tip Spacing Volume Range ul 2 125 10 300 50 1250 50 1250 0 5 12 5 1 30 2 125 0 5 12 5 1 30 2 125 Calibration Range ul 12 5 125 30 300 125 1250 125 1250 1 25 12 5 3 30 12 5 125 1 25 12 5 3 30 12 5 125 Color Code Color Code Color Code Compatible ClipTip ClipTip 12 5 ClipTip 200 ClipTip 300 ClipTip 1250 Compatible ClipTip ClipTip 12 5 ClipTip 200 ClipTip 300 ClipTip 1250 ClipTip 12 5 ClipTip 200 ClipTip 300 ClipTip 1000 ClipTip 384 12 5 ClipTip 384 30 ClipTip 384 125 Compatible ClipTip ClipTip 200 ClipTip 300 ClipTip 1250 ClipTip 1250 ClipTip 384 12 5 ClipTip 384 30 ClipTip 384 125 ClipTip 384 12 5 ClipTip 384 30 ClipTip 384 125 Thermo Scientific E1 ClipTip User Manual 7 A 2 Safety 1 P P P P 8 Thermo Scientific E1 ClipTip User Manual A 2 1 Intended Use The intended use of the device is to transfer liquids with different properties accurately and reliably The E1 ClipTip pipette and ClipTip tips are designed to be part of an analyzing system for an end user who is respon
32. 1250 00 160 1 44 75 0 60 Cliplip 1250 625 00 9375 150 3 75 0 60 125 00 50 400 1 88 1 50 4671010 12 05 125 12 50 0 313 2 50 0 200 1 60 Cliplip 12 5 6 25 0 156 250 0 150 240 1 25 0 150 17200 0 150 12 00 4671050 12 240 1250 125 00 2 50 2 00 075 060 ClipTip 200 62 50 1 25 2 00 0 80 1 28 12 50 1 00 8 00 050 400 4671080 12 ch 1040 3000 300 00 6 0 2 00 1 8 0 60 Clilip 300 150 00 3 0 2 00 12 0 80 30 00 1 5 500 0 6 2 00 Thermo Scientific E1 ClipTip User Manual 85 4671030 4671020 12 ch 16 ch 30 0 850 0 09129 850 00 425 00 30 00 12 50 12 5 6 3 5 40 0 313 1 50 1 50 4 71 2 50 ClipTip 1000 ClipTip 12 5 4671030 16 ch 1 0 30 0 6 25 1 29 30 00 50 156 50 150 0 60 2 50 12 00 2 00 384 ClipTip 30 15 00 3 00 0 30 0 30 2 00 10 00 384 1 7 00 4671060 16 ch 2 0 125 0 125 00 62 50 12 50 2 50 1 25 1 00 2 00 2 00 8 00 J 0 60 2595 0 60 1 7 2 00 0 200 1 60 0 150 240 0 150 12 00 0 27 0 90 0 30 2 00 0 2 0 75 0 60 0 980 1 28 0 50 4 00 E1 ClipTip Adjustable Tip Spacing Equalizer Multichannel models Cat No 4672050 Channels imprecision std pl CV Cliplip 125 384 ClipTip 96 format 8 ch 4672080 0 75 0 80 0 50 0 60 1 28 4 00 1 8 0 50 ClipTip 200 96 format 8 ch 4672090 E 0 6 0 80 2 00 ClipTip 300 96 format 6 ch 4672100 7 2 3 5
33. Add new calibration and press 7 Add 4 Select the type of calibration using gt and J either One point or Two point calibration 5 Scroll to Name Edit the default name using V Rename This opens the character window where the cursor can be moved using the arrow keys Enter a highlighted character to the name by pressing Select Press SQ Done when finished 6 Press S899 Done 7 In one point calibration set the Target volume using gt di or use the Volume editor After the Target volume set the Actual volume to correspond to the obtained calibration volume 8 In two point calibration the Target volume levels are fixed Change the Actual volumes to correspond to the obtained calibration volumes 9 When the changes are ready press 899 Done 10 A window opens allowing the user to press the trigger 11 A confirmation window appears Accept with 8 Yes 12 new adjustment setting has been saved to memory he stored special adjustments can be used in the Programs mode where they can be connected to user created programs section D 2 3 After connecting the special adjustment to a program perform re calibration of the device according to section E 4 2 to verify the device performance Note he acceptance specifications in section E 4 2 apply only when water is dispensed with the Forward pipetting technique Note he volume conversion factor Z in section E 4 3 1 applies only to water Conversion calculati
34. E 5 3 for more information E 5 1 Changing Default Calibration Setting All Presets functions are affected when the Default adjustment setting is changed he user may edit this setting excluding the name The Default calibration setting is always a 2 point calibration Calibration volumes are 1096 of the nominal volume and the nominal volume Note he user may lock the Default calibration setting with a password so that the setting cannot be changed without authorization See section D 3 1 4 for more information Thermo Fisher Scientific EE Default calibration Changing Default calibration affects all Presets pipetting functions Default calibration 300 00Uly Actual min 30 00uI Volume editor Done mm Thermo Fisher Scientific Note he user may check the date of the last made Default calibration setting from the Calibration tracker See section D 3 2 3 for more information Note t is advised that the performance values for the Default calibration setting are defined according to ISO 8655 procedures in the Forward mode Caution When changing the Default calibration settings all functions under Matrix and Presets are affected 1 Select Settings and press WV Select 2 Select Calibrate and press Select 3 Select Default calibration and press WV Edit 4 A notification will appear to warn about influencing all Presets functions Accept with W Ok 5 Change the Actual m
35. Nase DNase ATP and Pyrogens Does not apply to empty racks Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 95 Info pipettes thermofisher com www thermoscientific cliptip www thermoscientific com thermoscientific com 2013 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Fisher Scientific Ratastie 2 P O Box 100 01621 Vantaa Finland Thermo SCIENTIFIC 1508890 01 Part of Thermo Fisher Scientific
36. Note According to the 1508655 the acceptance specifications mentioned in this document apply only in the Forward mode Typical performance values for the Stepper mode can be inquired from the manufacturer E 4 3 Calculation Formulas E 4 3 1 Volume Calculation V w e x Z V volume ul w mass mg Z conversion factor ul mg e evaporation loss mg Fvaporation loss can be significant with low volumes To determine mass loss dispense water into the weighing vessel note the reading and start a stopwatch See how much the reading decreases during 30 seconds e g 6 mg 0 2 mg s Compare this to the pipetting time from tareing to reading Typically the pipetting time might be 10 seconds and the mass loss is 2 mg 10 s x 0 2 mg s in this example If an evaporation trap or lid on the vessel is used the correction of evaporation is usually unnecessary he factor Z is for converting the weight of the water to volume at the test temperature and pressure A typical value is 1 0032 ul mg at 22 C 95 kPa See the conversion table in Appendix 1 The value of the Z conversion factor depends on environmental conditions Appendix 1 Select the factor from the table according to the air pressure and water temperature recommended The volume is more dependent on the water temperature than the air temperature 4 3 2 Inaccuracy A V V A Inaccuracy y average volume Vs target volume display value ACC 100
37. P 4651170 F1 ClipTip tal fixvol S 4651180 jFi Cipripsulfixvo 4651190 jFiClipripiOdfixvo f 4651200 F1 ClipTip 20pl fixvol J 4651210 Fi ClipTip 25ulfixvol J e 4651220 Fi ClipTip 50ulfixvol f 4651230 F1 ClipTip 100p fixvol f 4651240 F1 ClipTip 200ulfixvol J 4651250 Fi ClipTip 250ul fixvol 4651260 FiCliprpsOOufixvo T EN Multichannels as ltir 4661120 FLCiprip amp ch550u j 4661130 1 8 ch10 100u1 Fel 4661140 1 8 ch 30 300u1 LJ Pej 4661150 F1 ClipTip12 ch1 1oul 4661160 1 12 5 5 L j 4661170 F1 ClipTip12 ch10 100n1 T LJ Peol 4661180 1 12 303000 T 92 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific EC en M 7 in E o Ln x 5 Pb Q Q Q Q Q Q Q Q a Order No Hesenpuon o 5 EE R Single channels Single channel 050 eJ oJ 467000 1 05 250 ei
38. User Manual The volume adjustment for different pipetting functions in the E1 ClipTip pipette can be done using three different methods Method 1 In this method the volume is adjusted by scrolling through the volume range 1 The volume can be changed by pressing V Edit or A while in the active pipetting function Picture D4 2 The Volume field will be highlighted Picture D5 3 Use 4 to Increase the volume and D to decrease the volume Volume scrolling can be accelerated by keeping the keys pressed 4 Press SQ Done when the changes are ready Method 2 In this method it is possible to adjust the digits of the volume number separately allowing a quicker setup from a low value to a high one or vice versa e g from 0010 to 1220 1 The volume can be changed by pressing V Edit or A v while in the active pipetting function Picture D4 2 The Volume field will be highlighted Picture D5 3 Press to activate the Volume editor Picture 06 4 Use vo Increase the selected number and A to decrease the number 5 Use gt d to highlight the next number to be edited D Press SQ Done when the changes are ready Method 3 In this method it is possible to select a volume from a recently used volume list The list is located on the right edge of the Volume editor window The maximum number of volumes in this list Is six Thermo Fisher Scientific Volume Done Picture D7 300 0
39. a mechanical locking solution E1 ClipTip pipettes feature two different locking types Both enable easy to use procedures to use the correct attachment force to the tips B 4 1 ClipTip 96 format Tips omall claws lock the tip into the tip fitting The claws prevent accidental tip detachment during pipette operations Note that the small volume ClipTip 12 5 is similar to the ClipTip 384 tips 4 2 ClipTip 384 384 format Tips ClipTip 12 5 and all ClipTip 384 tips feature a unique snap mechanism for tip attachment The tip has small incorporated protrusions that snap on the tip fitting flange ensuring light force and secure tip attachment The flexible plastic lip design seals the tip against the tip fitting All E1 ClipTip pipettes utilize an electronic tip ejection Two color coded buttons are located to enable both right and left handed persons to operate the system smoothly see section B 2 Note he tip ejection function is prevented whenever there is liquid inside the tip to avoid accidental ejection of tips Caution Always eject the tips into an appropriate waste container Do not point the ejecting tips at other persons Thermo Scientific E1 ClipTip User Manual 17 B 4 3 Tip Attachment 1 loattach the tip guide the pipette into a Cliplip in a rack and press with a light force until the tip is attached A delicate click sound will indicate that the tip 15 attached he tip is attached when the clips are locked into th
40. ax volume to correspond to the obtained calibration maximum volume using D d or the Volume editor 6 Scroll to Actual min volume and change the volume to correspond to the obtained calibration minimum volume using J or the Volume editor 7 Press P Done when the changes are ready 8 A confirmation window appears Accept with 7 Yes No will return you to the Calibrate menu 9 The Defaults calibration setting has been changed 10 Perform re calibration of the device according to section EA to verify the effect of new adjustment settings 11 Repeat Steps 1 7 if necessary Note f the volume settings are not changed when Done is pressed a notification text will appear Proceeding with Ok will return to the Calibrate menu Note f only one of the volume settings has been changed when Done is pressed a notification text will appear Proceeding with Yes will proceed to confirmation and will return to the Calibrate menu Thermo Scientific E1 ClipTip User Manual 61 1 EJE 62 Thermo Scientific E1 ClipTip User Manual E 5 2 Creating Special Adjustments The user can store up to 5 different special adjustments that can be used in created programs to improve the application specific device performance Created special adjustments only affect the programs that have been defined to use the specific setting 1 Select Settings and press Select 2 Select Calibrate and press 88 Select 3 Select
41. be edited Scroll to Program and press 7 Edit name Picture D31 To add a text see section D 3 1 1 on how to use the virtual keypad to change the name Press SQ Done when the changes are ready 5 The new program is linked to the Default calibration Different calibrations can be selected if special adjustment settings have been created section 5 2 Scroll to Calibration and press Calibration list Scroll to the intended adjustment setting and press Select The pipette returns to the program settings 6 Each program contains a set of values that need to be defined before use Press QQ Done when the changes are ready Note When the user changes the default adjustment setting to a user created adjustment setting the pipette indicates this with a balance symbol together with the name of the selected calibration in the display title field during the use of the program Editing an existing program 1 Select Programs and press WV Select 2 Scroll to the stored program to be edited 3 Use 5 a to highlight the 4 Each program contains a set of values that need to be defined before use icon and press WV Edit Press 89 Done when the changes are ready Deleting a program 1 Select Programs and press WV Select 2 Scroll to the stored program to be deleted 3 Use gt 6 highlight the icon and press 7 Delete Picture D32 4 Confirm deleting by pressing Yes Ther
42. been registered and a 1 year warranty for non registered ones 2 The warranty registration certificate and proof of purchase MUST be presented to Thermo Fisher Scientific or its distributor when making a claim for replacement 3 The customer must give notice to Thermo Fisher Scientific or its distributor in writing immediately after discovery of the defect 4 If requested the product must be returned to Thermo Fisher Scientific or its distributor The product must be adequately packed and fully insured and all shipping fees must be paid 5 Our responsibility extends only to product defects The warranty does not apply if in the opinion of Thermo Fisher Scientific the product has been damaged by accident misuse or chemical or physical abuse or by service or modifications by someone other than a service provider of Thermo Fisher Scientific 6 During the Warranty period non wearable parts are covered 10096 Non wearable parts are effectively parts that are designed to last for the life of the product Wearable parts are parts that will wear under normal operation such as O rings and battery These parts are not covered under the Warranty 7 Routine cleaning and recalibration are not covered under the terms of the Warranty 9 Validity may vary by country To check your country specific warranty claims go to www thermoscientific com pipettewarranty Quality Statement We hereby certify that all Thermo Scientific E1 ClipTip pipettes
43. connected in single channel pipette 64 Thermo Scientific E1 ClipTip User Manual Possible action Re attach tighten the tip fitting or replace with new tip fitting s using the tool Discard the tip Change the O ring Attach firmly Clean the tip cones and attach new tips Single channel pipettes Clean and grease the O ring and cylinder Multichannel pipettes Contact service Follow the instructions carefully Attach firmly Recalibrate according to the instructions Use the correct tip Tighten the tip fitting with the tool or replace the tip fitting set Open the clips manually with a small sharp tool and pull out the tip Tighten the tip fitting with the tool or replace the tip fitting set Recharge the battery Recharge the battery Single channel pipettes Remove the tip cone module Move the piston by hand or with the piston removal tool Re attach the module Multichannel pipettes Contact service Attach the module in service mode Thermo Fisher Scientific Defect Possible reason Possible action Not chargingthe Charger cable not attached properly Detach and reconnect properly battery to the pipette Charger not connected to Connect the charger to the mains voltage the mains voltage Pipette not placed properly into Take the pipette out from the charging the charging stand stand and place it back again properly Charger cable not connected to Connect the charger cable to the charging
44. ct the Matrix icon from the main menu 1 ou cr 2 Select ic Fill step is added Use the Left Right keys or the Volume editor to adjust the volume to 1250 pl Done volume is accepted Add Add a new step Use the Down key to highlight Dispense Select Dispense step is added This step is for discarding the first 50 ul Use the Left Right keys or the Volume editor to adjust the volume of 50 pl Done he volume is accepted Add Add a new step Use the Down key to highlight Multi Select W Multi step is added INO del 52 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific Use the Left Right keys or the Volume editor to adjust 13 gt the volume to 100 pl 14 Use the Up Down keys to highlight Repetitions Use the Left Right keys to adjust the repetitions to 12 Done The volume is accepted Done The editing is ready 18 lt Ok b d Purge step is added Done The function is ready to run Simple Dilutions Example Prepare a 1 10 dilution with a 10 pl sample volume Note he first fill volume is for the diluent followed by the air gap volume and finally by the sample volume Note lenfold serial dilutions can be done by repeating this example multiple times E Using Presets 1 Select Select the Presets icon from the main menu n m un m cr un Use the Up Down keys to highlight Dilute
45. cts all Presets and Matrix pipetting functions Probable cause Battery voltage level is low special calibration that was used in the program in question has been deleted Battery power was lost because battery is empty or battery connector was detached Pipette has been re started unintentionally because of low battery level or some other unexpected event Default calibration has been selected to be adjusted Contact service for a replacement battery Do not use the pipette with a defective battery Using the pipette with a damaged battery caused by battery failure 3 may cause a potential safety risk thermal runaway Repeat the Connect piston function according to the instructions If it fails again then contact service Solution Charge the pipette See the instructions If the special calibration was deleted unintentionally then it has to be recreated See the Instructions Press the trigger to the empty tips Caution f there is liquid in the tips It will be dispensed Notice also that the previous dispensed dose may be inaccurate Press the trigger to empty the tips Caution If there is liquid in the tips it will be dispensed Notice also that the previous dispensed dose may be inaccurate Be aware that Default calibration Is used in all Presets functions and the Matrix function and in all of the user programs that are not using special calibrations Thermo Scientific E1 ClipT
46. d the dilution ratio define the diluent fill 1 and sample fill 2 volumes automatically The diluent fill 1 is the larger of the volumes In the volume based method the volumes are set directly This volume based method uses user defined diluent fill 1 and sample fill 2 volumes Press 88 Edit in the pipetting mode view Use gt a to select between ratio based and volume based dilution Ratio based 1 Scroll to Ratio and change the value using gt 4 2 Scroll to Total volume and change the volume using 4 or the Volume editor section D 1 2 Fill 1 and Fill 2 volumes will be calculated automatically Thermo Fisher Scientific 3 Scroll to Fill 1 speed and change the value using e 4 Dilute Mix 4 Scroll to Fill 2 speed and change the value using gt d Mix type 5 Scroll to Speed out and change the value using gt 2 6 Scrollto Mix type and select between User controlled Cycle based using gt d 7 If Mix type is cycle based scroll to Mix cycles change the value using 5 a 8 Scroll to Mix speed and change the value using gt d 9 Scroll to Counter and select Counter off on using gt a Picture D26 Picture D26 10 Press SQ Done when the changes are ready Volume based 1 Scroll to Fill 1 volume and change the volume using gt a Or the Volume editor section D 1 2 2 Scroll to Fill 2 volume and change the volume using D 4 Or the Volum
47. d to use an environmental monitoring device to assure the reliability of condition values E 4 2 Procedure Note the temperature water temperature recommended and air pressure values before testing select the Z correction coefficient value from Appendix 1 1 Take a new tip from the tip rack box using the pipette Do not touch the tips by hand 2 Pre wet the tip 3 5 times 3 Dispense 10 doses at 1096 of the nominal volume 4 Dispense 10 doses at the nominal volume 5 Calculate the inaccuracy A and ACC and imprecision s and CV of both series see section D 4 3 6 Compare the results to the limits in Appendix 2 or 3 If the calculated results are within the selected limits the adjustment of the pipette Is correct With multichannel pipettes calibration can be performed either to all channels standard demand or for the edge channels of the device A pipette should always be adjusted for delivery Ex of the selected volume Measuring volumes taken from the balance are not allowed Calibration should be performed using the forward pipetting method For maximum permissible errors see Appendix 2 Manufacturer Factory specification Limits and Appendix 3 1508655 Calibration Specification Limits Thermo Fisher Scientific Thermo Fisher Scientific EE Note Inaccuracy and imprecision calculations can also be done using the Pipette Calibration Calculator that can be found on the Thermo Scientific web pages
48. daily it should be checked every three months Fitting and Sealing Ring s Caution The E1 ClipTip sealing rings 6 and 7 are wear parts Clean them after contamination use of aggressive chemicals or heavy stress Replace the sealing rings if they are worn or damaged he servicing procedure starts with the disassembly of the pipette A Picture G1 Removing and replacing tip fitting s 5 and sealing ring s 6 and 7 in single channel and multichannel pipettes Single channel and multichannel models from 2 125 to 50 1250yl with 96 format tip fitting 1 To remove the tip fitting place the tool end without a metal rod of the tip fitting tool C against the tip fitting The tip fitting will guide the tool properly into place Turn the tool counterclockwise to untighten the tip fitting Rotate the tip fitting tool C and press the end with a metal rod through the tip fitting Take care not to damage the O ring 7 inside the system with excess force Loosen the tip fitting with a tool and pull it out Make sure that the O ring 7 comes out with the tip fitting See Picture G3 and Appendix 4 i Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 69 A 10 Thermo Scientific E1 ClipTip User Manual 2 Remove the used sealing ring 6 e g using the point of a disposable tip 3 Place a new sealing ring by rolling it into place 4 Make sure that the O ring 7 is intact 4 Place the tip fitting onto the t
49. ded for aquaeous solutions such as buffers diluted acids or alkalis A mixing step is added after dispensing of the liquid To use the mode 1 Select Presets and press WV Select 2 Select Forward Mix from the list and press Use 3 Place the tip s under the liquid surface and press the trigger to aspirate the liquid 4 Press the trigger to dispense the liquid 5 Place the tips under the surface of the liquid Press the trigger to start mixing a User controlled mix type As long as the trigger is pressed down ca 70 of the total volume is pipetted Picture D22 6 b Cycle based mix type The set amount of mixing cycles is done by the pipette 7 Next the display shows Purge Press the trigger to empty the tip purge To change the settings 1 Press 88 Edit in the pipetting mode view 2 Change the volume using gt a or the Volume editor section D 1 2 3 Scroll to Speed in and change the value using e 2 4 Scroll to Speed and change the value using L a 5 Scroll to Mix type and select between User controlled and Cycle based using 5 a 6 If Mix type is cycle based scroll to Mix cycles and change the value using gt di Picture D23 7 Scroll to Mix speed and change the value using D d 8 Scroll to Counter and select Counter off on using 2 a 9 Press SQ Done when the changes are ready Thermo Scientific E1 ClipTip User Manual 37 Dilute Mix
50. e G 3 4 Multi Channel Pipettes user may remove and clean the tip fittings and sealing rings of 96 format multichannel pipettes and change them as described in section G 2 if found necessary 384 format multichannel pipettes cannot be disassembled by the user Only the outside surfaces can be cleaned In case of malfunctioning or a suspicion of damage to the lower parts of the pipette contact the closest service representative Chapter J TECHNICAL SERVICE Caution Servicing of the multichannel pipette handles should only be performed by trained and authorized personnel Disassembly of multichannel pipettes may cause malfunctioning of the device If not executed by a professional Thermo Scientific E1 ClipTip User Manual 73 G 4 The display keypad handle and lower parts of the E1 ClipTip pipette as well as _ _ the tip fittings of 384 model multichannels can be wiped with decontaminants Decontamination recommended below These parts must not be immersed in liquid Procedure Removable tip fittings see sections G2 and G3 for disassembly can be immersed in the decontaminant solution according to the table below Afterwards the parts must be thoroughly rinsed with distilled water and allowed to dry before reassembling Recommended decontamination solutions for the E1 ClipTip pipette Display and Lower parts Tip fittings keypad and sealing rings Ethanol 7096 Caution he effectiveness of the decontamination
51. e ClipTip Filter 1000 sterile ClipTip Filter 1250 sterile ClipTip Filter 384 12 5 sterile ClipTip Filter 384 30 sterile ClipTip Filter 384 125 sterile ClipTip Filter Blister Tub Reloads Order number 94420218 94420258 94420318 94420518 94420718 94420818 Description ClipTip 20 Filter Reload sterile ClipTip 50 Filter Reload sterile ClipTip 200 Filter Reload sterile ClipTip 300 Filter Reload sterile ClipTip 1000 Filter Reload sterile ClipTip 1250 Filter Reload sterile Volume range 0 5 12 5 ul 1 20 ul 5 50 ul 5 200 ul 10 300 ul 30 1000 ul 50 1250 ul 0 5 12 5 ul 1 30 ul 2 125 ul Volume range 1 20 ul 5 50 ul 10 200 ul 30 300 ul 100 1000 ul 50 1250 ul Color Coding Pink Pink Violet Yellow Orange Blue Turquoise Pink Violet Yellow Color Coding Pink Violet Yellow Orange Blue Turquoise Oty 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 10 x 96 rack 8 x 96 rack 8 x 96 rack 10 x 384 rack 10 x 384 rack 10 x 384 rack Oty 10 x 96 insert 10 x 96 insert 10 x 96 insert 10 x 96 insert 8 x 96 insert 8 x 96 insert ClipTip Empty Racks 94410219 94410519 94410819 ClipTip Empty Rack Small ClipTip 20 ul and 50 ul ClipTip 200 ul and 300 ul ClipTip 1000 and 1250 ul 10 x 96 rack no tips inserts ClipTip Empty Rack Medium 10 x 96 rack no tips inserts ClipTip Empty Rack Large 8 x 96 rack no tips inserts All ClipTip tips are certified to be free of R
52. e into a 96 well microplate Picture B8 Thermo Fisher Scientific C RECOMMENDATIONS FOR ACCURATE PIPETTING For optimal pipetting results we advise you to follow the following If possible make sure that the pipette tips and liquid are at the same temperature Pre rinsing the tip three to five times with the liquid to be pipetted improves accuracy and precision Pre rinsing Is especially important when pipetting volatile compounds since It prevents liquid from dripping out of the tip During aspiration it is recommended to wait until the liquid movement in the tip s stops before withdrawing the tip from the liquid After aspiration and dispensing it is recommended to touch the tip s against the edge of the reservoir to remove any possible remaining liquid on the outside surface of the tip samples should be pipetted in the same manner aspiration depth pipetting angle preferably in the upright direction and dispensing technique dispensing into air immersed into liquid or touching the wall Aspiration and dispense at low speeds when working with high viscosity liquids pipette can be adjusted for liquids of a different density or viscosity to improve accuracy and precision See Chapter E CALIBRATION AND ADJUSTMENT for further information To improve pipetting results in multi dispensing operations stepper and multi functions it is recommended to use a pre step In the stepper mode the default settin
53. e editor section D 1 2 3 Scroll to Fill 1 speed and change the value using d 4 Scroll to Fill 2 speed and change the value using gt a 5 Scroll to Speed out and change the value using 4 6 Scroll to Mix type and select between User controlled and Cycle based using gt 2 7 If Mix type is cycle based scroll to Mix cycles and change the value sn P 8 Scroll to Mix speed and change the value using d 9 Scroll to Counter and select Counter off Counter on using gt d 10 Press SQ Done when the changes are ready Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 39 Manual Picture D28 40 Thermo Scientific E1 ClipTip User Manual D 2 2 8 Manual The Manual mode can be used in different applications It can be used for Instance to evaluate the amount of remaining liquid in the reservoir or to dispense small amounts of liquid when slow speeds are needed e g gel electrophoresis The volume reset function can be utilized for instance in titration Note he Manual mode contains slower speed options than the other operation modes To use the mode 1 Select Less Presets and press WV Select 2 Select Manual from the list and press Y Use 3 Press the trigger to aspirate the liquid 4 The display indicates the volume aspirated into the tip 5 Liquid is aspirated for as long as the trigger is kept pressed or until the Limit volume has been reached he
54. e tip fitting Do not use excessive force when attaching the tips as the device requires minimum attachment force 2 Lift the pipette 3 If the tip is not attached repeat Steps 1 and 2 B 5 Adjustable Tip The E1 ClipTip Equalizer pipette allows one to change the tip to tip spacing This enables pipetting into or between vessels of varying center to center Spacing configurations E1 ClipTip Equalizer Adjustable Tip Spacing Multichannel models EqualizerWindow Tip Spacing Volume 48 24 Color Compatible Cat No Channels mm Range ul Ze Tube Code ClipTip 8 2 0 125 ClipTip 200 10 0 300 50 0 1250 ClipTip 300 ClipTip 1250 ClipTip 1250 ClipTip 384 12 5 ClipTip 384 30 ClipTip 384 125 ClipTip 384 12 5 ClipTip 384 30 ClipTip 384 125 50 0 1250 0 5 12 5 1 0 30 2 0 125 0 5 12 5 1 0 30 2 0 125 Equalizer Window Spacing mm Application 384 well microplates 384 4 5 304 PCR plates Agarose gels 96 well microplates Jb Deepwell blocks 48 well microplates 48 13 Deepwell blocks MCT Microcentrifuge tube racks 24 18 Test tube racks Tube 24 well microplates 18 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific B 5 1 To Set the Stop Buttons to Match the Application There are three ways to set the stop buttons in the correct positions for application when using the adjustable tip spacing Stop button maximum Stop button minimum N T Indicator line Spacing
55. ect Select the Presets icon from the main menu n m un m cr un Use the Up Down keys to highlight Forward Use Select the function to use Edit Edit the function Use the Left Right keys or the Volume editor to adjust the volume of 100 pl Done The volume is accepted and the function Is ready to run ez re Using Matrix ES Select Select the Matrix icon from the main menu Select Fill step is added k tu Use the Left Right keys the Volume editor to adjust the volume to 100 ul Done The volume is accepted Done The program is ready Ok b 4 Purge step is added C GE Done The function is ready to run N Thermo Scientific E1 ClipTip User Manual 51 Serial Dispensing Example Fill a 96 well plate with 100 ul per well using an 8 channel 1250 ul pipette Using Presets 1 Select Select the Presets icon from the main menu m un m un Use the Up Down keys to highlight Stepper Use Select the function to use E Edit Edit the function Use the Left Right keys or the Volume editor to adjust the volume of 100 pl Use the Up Down keys to highlight Repetitions d 8 Use the Left Right keys to adjust the repetitions to 12 Done The function is ready to run Note t is recommended to set Pre step to In use in the Stepper function Using Matrix Select Sele
56. ed the shortcut will be updated automatically When a stored program is deleted a program shortcut is also deleted D 3 1 3 Organize Icons In this mode it Is possible to organize the icons in the main menu enabling fast access to the most used functions 1 Select My Pipette and press 7 Select 2 Select Organize icons and press 88 Select 3 Move the cursor over to the desired icon using the A v and 2 d keys Press Move to select a desired icon 4 Arrow symbols appear within the cursor frame around the icon to be moved Picture D38 Move the icon using the v and A keys to a desired place in the menu and press 7 Ok The icon has now been moved to a new location in the main menu D Press SQ Ready after the changes have been done Thermo Scientific E1 ClipTip User Manual 45 D 3 1 4 Password Protection It is possible to protect against editing certain items with a password as with all created programs located under the Programs main function Also the default calibration and special made calibrations located under the Settings function can be protected with a password In addition under the My Pipette main function the Pipette name Shortcuts and Organizing of icons can be protected Use the same set password for all selected protections 1 Select My Pipette and press 88 Select 2 Select Password protection and press 8 Select Password protection 3 Press 88 Select to set the
57. ed that many premises do not have the possibility to meet these requirements Note he calibration procedure is also the same under nonconforming conditions however the manufacturer specifications or the 150 8655 standard specifications do not apply in these cases E 4 1 Device Requirements and Test Conditions e Ananalytical balance should be used The scale graduation value of the balance should be chosen according to the selected test volume of the pipette Minimum requirements for balances according 10150 8655 Tested volume area Resolution Repeatability Standard uncertainty of linearity measurement 1 ul V 10 ul 0 001 0 002 10 pl lt V lt 100 p 100 pl lt V lt 1000 yl lt 10 Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 57 58 Thermo Scientific E1 ClipTip User Manual If the uncertainty of the measurement of the balance is known this may be used instead of the repeatability and linearity e est liquid Distilled or deionized water conforming to the 150 3696 grade 3 requirements e Tests should be done in a draft free room at a constant 0 5 C temperature of water pipette and air between 15 C to 30 C e relative humidity should be above 50 Especially with volumes under 50 ul the air humidity should be as high as possible to reduce the effect of evaporation loss Special accessories such as an evaporation trap are recommended Note It is recommende
58. ermo Scientific ET ClipTip User Manual Thermo Fisher Scientific Using Matrix Select Select the Matrix icon from the main menu Matrix Select Fill step is added Use the Left Right keys or the Volume editor to adjust the volume to 150 ul Done The volume is accepted Add Add a new step Use the Down key to highlight Dispense Select Dispense step is added Use the Left Right keys or the Volume editor to adjust the volume of 30 pl Done The volume is accepted Add Add a new step Use the Down key to highlight Timer edd raoaea 12 Select step is added 13 D a Use the Up Down and the Left Right keys to set A the timer to 30 s Done The timer setting is accepted e Repeat Steps 5 to 14 three times in order to create three more 15 dispense and timer steps he last dispensing will be done with the Purge step Done The editing is ready b Ok y Purge step is added Done The function is ready to run Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 55 E 1 Terminology E 2 Factory Calibration 56 Thermo Scientific E1 ClipTip User Manual E CALIBRATION AND ADJUSTMENT Calibration Determination of the difference between the actual volume given by the device and the target volume Also enables statistical evaluation of the deviation between individual doses Adjustment Altering the pipette settings to change
59. g for the pre step is In use In step based programs the user needs to program a separate dispensing step as the pre step Do not clean tips for reuse as their metrological characteristics will no longer be reliable lips are designed for single use only Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 21 22 Thermo Scientific E1 ClipTip User Manual Note Pipetting performance cannot be guaranteed if the tip is reused Choose only a tip and pipette of matching color See information on compatibility In section A 1 1 Note When dispensing into liquid the trigger should be kept pressed until the tip is withdrawn from the liquid to prevent aspiration of the liquid Caution he user should check that the device is resistant to liquids that are intended to be handled The user should also verify the resistance to the used cleaning methods Thermo Fisher Scientific D PROGRAMMING AND OPERATION D 1 User Interface The E1 Cliplip user interface contains Icons on the main menu showing six I main functions Picture D1 The main menu appears when the pipette is vervIeW tured on and also when the pipette is initiated from the power save mode see the detailed menu map in Picture D2 All pipetting actions are included in the Matrix and Presets icons Matrix and Presets are designed for fast and easy pipetting without saving The last used parameters will be saved automatically Matrix The Matrix function uses
60. haracter can be deleted by selecting the desired type slot on the upper row of the virtual keypad 4 P ab Ab AB 12 by using the gt d and A v keys and pressing Y Select 5 Different key functions are defined in the table below 6 Press SQ Done after entering the desired name or text Thermo Scientific E1 ClipTip User Manual 43 Virtual keypad functions AB 1 2 44 Thermo Scientific E1 ClipTip User Manual Move the highlight within the keypad in a horizontal direction Move the highlight within the keypad in a vertical direction The Left selection key Select confirms the virtual keypad selection The Right selection key Done confirms the contents of the editor field and stops editing Move the cursor left right in the editor field text Deletes a character to the left of the cursor in the editor field Changes the characters in the keypad to lowercase letters The uppercase keypad Is used at the beginning and after a full stop otherwise the lowercase keypad Is used Changes the characters in the keypad to uppercase letters Changes the characters in the keypad to numbers and special characters D 3 1 2 Shortcuts This mode enables the user to create icon shortcuts and place them in the main menu view to provide quick access to favorite programs and pipetting functions Select a shortcut icon to quickly start the program or pipetting function without scrolling in menus or folders There are four
61. he changes are ready 0 2 2 4 Repetitive The Repetitive mode Is recommended when the reverse pipetting technique Is needed for a few repeated dosings of the same volume This is particularly useful when working with volumes close to the maximum volume of the pipette To use the mode 1 Select Presets and press WV Select 2 Select Repetitive from the list and press 7 Use 3 Place the tip s under the liquid surface and press the trigger to aspirate the liquid Thermo Fisher Scientific p Dispense 300 0ul Picture D18 Remove tips from liquid Fill 2 15 0pl Picture D19 Thermo Fisher Scientific 4 Press the trigger and keep it pressed to dispense the liquid Touch the tip against the edge of the reservoir to remove any possible remaining liquid on the outside surface of the tip An excess volume will remain inside the tip 5 Return to the original liquid reservoir and place the tips below the surface Release the trigger to aspirate 6 Repeat Steps 4 and 5 as long as needed 7 Press the trigger shortly with the last dose 8 he display shows Purge Press the trigger to discard the excess volume To change the settings 1 Press 88 Edit in the pipetting mode view 2 Change the volume using gt d or the Volume editor section D 1 2 3 Scroll to Speed in and change the value using D 9 4 Scrollto Speed out change the value using gt q 5 Scroll to Counter and select Coun
62. he trigger down to connect the motor coupler to the piston Model 50 1250jl 1 Place the spring support 10 spring 11 support 13 and sealing ring 15 on the piston assembly 12 2 Carefully slide the assembly into the tip cone 9 3 Insert the entire assembly into the handle and turn it tight by hand Use the tool A to lightly tighten the cylinder into the handle Insert the tip ejector assembly 8 into the handle and turn until the assembly drops into the correct place When correctly positioned the tip ejector only rotates 45 Make sure that the tip ejector Is turned in the clockwise direction until it stops You should feel a snap when the tip ejector Is correctly placed 5 Insert the tip fitting 2 as described in section G 2 6 Select Z Settings and press WV Select in the main menu of the pipette 7 Select Connect piston from the list and press Select 9 Press and hold the trigger down to connect the motor coupler to the piston Thermo Fisher Scientific Thermo Fisher Scientific gt P Caution he user must make sure that the piston sealing rings are intact before assembly and replace them if necessary Failing to check this may cause leakage of the pipette Caution The user must make sure that the small tip fitting O ring 7 is in place before attaching the tip fitting he tip fitting tool or a disposable tip can be used to place the O ring into place Failing to check this may cause leakage of the pipett
63. he volumes set directly This volume based method uses user defined diluent fill 1 and sample fill 2 volumes Press Edit in the pipetting mode view Use gt d to select between ratio based and volume based dilution Picture 020 Volume based 1 Scroll to Fill 1 volume and change the volume using D 4 or the Volume editor section D 1 2 2 Scroll to Fill 2 volume and change the volume using a or the Volume editor 3 Scroll to Fill 1 speed and change the value using 9 4 Scroll to Fill 2 speed and change the value using gt 4 5 Scroll to Speed and change the value using 2 D Press SQ Done when the changes are ready Ratio based 1 Scroll to Ratio and change the value using L a Picture D21 2 Scroll to Total volume and change the volume using D 4 or the Volume editor section D 1 2 Fill 1 and Fill 2 volumes will be calculated automatically 3 Scroll to Fill 1 speed and change the value using gt a 4 Scroll to Fill 2 speed and change the value using gt a 5 Scroll to Speed out and change the value using gt d D Press SQ Done when the changes are ready Note he volume and the speed of the air gap are defined automatically Thermo Fisher Scientific Forward Mix Keep trigzer pressed ze 10 Picture 022 Forward Mix Cycle based Picture 023 Thermo Fisher Scientific D 2 2 6 Forward Mix The Forward mode Is recommen
64. il it stops Thermo Scientific E1 ClipTip User Manual 71 12 Thermo Scientific E1 ClipTip User Manual 7 Use the tool A to lightly tighten the cylinder into the handle 8 Insert the tip ejector assembly 8 into the handle and turn clockwise until it stops You should feel a snap when the tip ejector 15 correctly placed 9 Insert the tip fitting b as described in section G 2 10 Select L Settings and press WV Select in the main menu of the pipette 11 Select Connect piston from the list and press WV Select 12 Press and hold the trigger down to connect the motor coupler to the piston Models 2 125yl and 10 300 1 Place the spring support 10 spring 11 support 13 and sealing ring 15 on the piston 12 2 Carefully slide the assembly into the tip cone 9 3 Insert the entire assembly into the handle and turn it tight by hand Use the tool A to lightly tighten the cylinder into the handle 4 Insert the tip ejector assembly 8 into the handle and turn until the assembly drops into the correct place When correctly positioned the tip ejector only rotates 45 Make sure that the tip ejector Is turned in the clockwise direction until it stops You should feel a snap when the tip ejector Is correctly placed 5 Insert the tip fitting 5 as described in section G 2 6 Select Z Settings and press Select in the main menu of the pipette 7 Select Connect piston from the list and press Select 9 Press and hold t
65. ime to the pipette Follow the instructions on the display 3 Press the trigger switch to initialize home positioning See section B 2 for more information about control buttons 4 The pipette will show the main menu and is ready for use 5 The pipette can be turned off by selecting the Power icon from the main menu A 3 6 Power Saving If the pipette is not used for a minute the brightness of the display is decreased and after 10 minutes the screen saver mode Is entered Exit the screen saver by pressing one of the selection keys or arrow keys A D 4 Home positioning needs to be initialized again after screen saving and is done by pressing the trigger switch If the pipette is not used for an hour it will automatically turn itself off Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 13 B PIPETTE OVERVIEW B 1 Components 1 Connector Socket For connecting the power supply 2 Battery Case Case for the Li ion battery See section A 3 1 for installation 3 Display LCD backlit color display 4 Charging Pins Charging pins to connect the pipette to the charging stand See section A 3 4 5 Keypad For interacting with the pipette user interface See section B 2 6 Tip Ejection Keys For ejecting pipette tips 1 Pipetting trigger Trigger button to operate the piston movement See section B 2 8 Finger Hook Adjustable finger hook See section B 2 9 Tip Ejector 10 Ti
66. indicator qualizer window pacing adjuster m Using the millimeter scale When the user knows the tip spacing of the application in millimeter range 1 Depress the stop buttons while moving them along the scale 2 Set the Stop button minimum and maximum along the spacing indicator scale Match the Indicator line with the desired millimeter spacing 3 Helease the stop buttons to lock the positions for use Using application formats We recommend using the Equalizer window when the application formats are known 1 Depress the stop buttons while moving them to the outmost positions 2 Slide the Spacing adjuster to view the alternatives in the Equalizer window 3 Depress the Stop button minimum and move it to the edge of the Spacing adjuster with the selected format min in the Equalizer window with the marker dot visible 4 Release the stop button to lock the positions for use 5 Depress the Stop button maximum and move it to the other edge of the opacing adjuster with the selected format max in the Equalizer window with the marker dot visible 6 Release the stop button to lock the positions for use Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 19 Picture B7 e Immmmmmm TERIEN LARARE Picture B8 20 Thermo Scientific E1 ClipTip User Manual Using an unknown tip spacing If the tip spacing of the application is unknown for example when
67. ip User Manual 67 G MAINTENANCE G 1 Regular For reliable daily operation keep the E1 ClipTip pipette free of dust and away from liquid spills and Preventive Even though the E1 ClipTip pipette is constructed from high quality materials Maintenance you must Immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage It is recommended that you clean the case of the pipette periodically to maintain its good appearance soft cloth dampened with 70 ethanol solution is adequate It is also recommended to check the tightness of the tip fittings Caution Ethanol should not be sprayed directly on the pipette This may impair the transparency of the display window Use only a moistened cloth Caution he user should verify the suitability of any other cleaning reagents before proceeding It is recommended to use only 7096 ethanol for cleaning Caution Aggressive substances can damage the pipette or pipette parts Check for material compatibility before handling organic solvents and aggressive chemicals with the pipette Warning Use a decontamination solution if any surfaces are contaminated with biohazardous material Note lt is recommended to use filter tips to avoid contamination of the inner parts of the E1 ClipTip pipette gt gt gt 68 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific 1 2 Replacing Tip If the pipette Is used
68. ipTip User Manual 27 D 2 Pipetting Functions Add new step L 7 Cancel Picture D9 28 Thermo Scientific E1 ClipTip User Manual D 2 1 Matrix Function The Matrix function uses the step based operating technique Each pipetting step is entered separately in the sequence in which they will be performed Creating a new sequence e 1 Select the Matrix icon in the main menu A list of possible steps to be added to the pipetting sequence appears on the display Picture 09 2 Select the first step to be entered to the sequence by using v and by pressing 8 Select The selected step s editing window appears Picture 010 3 he step specific parameters can be edited the volume speed etc Press SQ Done when the changes are ready 4 o add a second or an additional step to the pipetting sequence highlight the Add new step row Picture 011 and press 889 Add D Press SQ Done when all the steps added to the pipetting sequence and the program is ready for use Matrix EE 1 Delete all steps Volume editor Done Picture D10 Picture D11 Thermo Fisher Scientific Matrix K Dispense 200 Du P Purge Add new step Delete all steps Picture D12 Thermo Fisher Scientific To use the mode 1 Select Matrix and press Y Select The pipetting mode view appears on the display 2 Place the tip s under the liquid surface and press the trigger to asp
69. irate the liquid 3 Continue until all the selected steps have been completed 4 Continue dispensing or press SQ Back to go back to the main menu Editing an existing program For each step it is possible to Picture D12 1 Edit step parameters Use QP d to highlight the fi WPF Edit Note that the tip ejection step cannot be edited Icon and press 2 Add a step above another step Use D to highlight tho KR icon and press WV Add above 3 Delete a step Use gt Gel to highlight theta icon and press 7 Delete All steps in the sequence can be deleted by highlighting the last row X Delete all steps and pressing Delete Available Steps The maximum amount of steps that can be added to one sequence is 20 The following steps can be selected by scrolling and pressing 8 Select Fill filling the tip with liquid Change the volume using J or the Volume editor section D 1 2 Scroll to Speed and change the value using gt d Press SQ Done when the changes are ready Dispense dispensing liquid Change the volume using D di or the Volume editor section D 1 2 ocroll to Speed and change the value using D 4 Press SQ Done when the changes are ready Thermo Scientific E1 ClipTip User Manual 29 Multi repeated dispensing of the same volume Change the volume using D g or the Volume editor section D 1 2 ocroll to Repetitions and change the number using eS a
70. isplays various messages order to guide and assist the user he most important notifications are listed and explained below Solution set the current date and time Press Ok and set the current date again Press the trigger to remove the friction or blockage Caution If there is liquid in the tips it will be dispensed Notice also that the previous dispensed dose may be inaccurate Contact service if the error reappears continuously piston needs to be greased Contact service piston needs to be greased Contact service Check that the connector of the battery Is attached properly Contact service for a replacement battery Do not use the pipette with a defective battery Contact service for a replacement battery Do not use the pipette with a defective battery Thermo Fisher Scientific Battery failure 3 Battery might be worn out outdated or damaged battery overvoltage detected he position sensor was not found Connect piston function in service menu of single channel pipette could not detect position sensor Display information A A Thermo Fisher Scientific Battery low Plug to charger Special calibration of this program has been deleted and will be replaced with the Default calibration Dispensing might be interrupted Press trigger to empty tips Dispensing interrupted Press trigger to empty tips Changing Default calibration affe
71. iston 12 Release the trigger 8 Turn the tip cone upside down and tap all the parts from it You can check the parts of each pipette model from the pictures in Appendix 4 Remember to keep all the parts in order on the table for reassembly G 3 2 Single Channel Pipettes Cleaning Clean the piston piston spring and sealing rings with a dry lint free cloth Clean the parts with a suitable cleaning solution by immersing item in a 7096 ethanol solution for example or wiping them with a dampened cloth Dry before assembly Grease the spring sealing ring and piston with the lubricant that comes with the pipette Caution Do not grease the tip fitting sealing ring 6 This may collect dirt and cause leaking of the pipette G 3 3 Single Channel Pipettes Assembly he pipette is assembled in the reverse order to disassembling See the model specific explosion pictures in Appendix 4 for assistance Model 0 5 12 5 1 Slide the spring 18 spring support 19 and sealing ring 20 on to the tube 17 2 Slide the spring support 13 tube 14 bigger sealing ring 15 smaller sealing ring 16 and previous tube assembly 17 on the piston 12 3 Carefully slide the assembly into the cylinder 9 4 Place the spring support 10 on to the other end of the spring 11 5 Place the free end of the spring 11 against the spring support 13 installed into the cylinder 6 Carefully slide the assembly to the handle and screw the cylinder clockwise unt
72. ly or upside down when there is liquid inside the tip The sample liquid may enter the pipette which may affect pipetting performance and impair the pipette Note Refer to section G 5 Autoclaving for autoclaving specific portions of the pipette or call Thermo Fisher Scientific Technical Service for instructions A 3 1 Installing the Battery The E1 ClipTip pipette is delivered with a detached battery Install the battery as follows Warning Incorrect handling of the battery or pipette may cause personal injury Do not drop the battery Do not expose the battery to any kind of mechanical stress or extreme temperatures above 60 C 45 C when charging or under 0 C 1 Open the battery lid press A and slide BJ 2 Slide in the battery with the label side visible 3 Connect the battery connector Note that the connector can be attached in two different orientations both of which are acceptable 4 Check that the battery wires are as shown in the picture 5 Slide back the battery lid as shown in the picture Thermo Fisher Scientific Thermo Fisher Scientific gt A A A 3 2 Charging the Pipette The charging time to full capacity is approx 2 h An indicator on the LCD display shows the charge level of the battery Battery full Battery empty ul Battery charging the level indicator scrolls down and up When the indicator shows that the battery Is empty pipetting is no more possible and the pi
73. mo Fisher Scientific Programs F Add new program 21 Menu Picture D33 gt gt D 3 Supporting Functions 04 07 PM 10 21 2013 E1 ClipTip ET Cp 4 Ab s 1 2 b d Done Picture D35 Thermo Fisher Scientific Using a stored program 1 Select Programs and press WV Select 2 Scroll to the stored program to be used 3 Use gt 9 to highlight the B icon and press 8 Use Picture D33 Caution he user is responsible for the validity of the created pipetting sequence in the Matrix and Programs functions Caution Ensure that the charge level of the battery is high enough especially when using a longer pipetting sequence in the Matrix function D 3 1 My Pipette It is possible to personalize the E1 ClipTip pipette user interface in the My Pipette function D 3 1 1 Pipette Name It is possible to give the E1 ClipTip pipette a personal name in this mode he name is shown in the main menu title field and on the display when the pipette is in sleep mode Picture 34 ET ClipTip is used as a default name To change the default name 1 Select w Pipette and press Y Select 2 Select Pipette Name and press 88 Select 3 he display shows a virtual keypad that is used to edit the name Picture D35 4 oedit the text in the editor field move the cursor in the virtual keypad and enter a character pressing Select The character set can be changed or a c
74. on Limits E1 ClipTip Single Channel models Thermo Fisher Scientific Cat No Range Volume Inaccuracy Imprecision ClipTip pl pl std ul CV 4670000 0 5 12 5 12 50 0 200 1 60 0 100 0 80 ClipTip 12 5 6 25 0 200 3 20 0 100 1 60 1 25 0 200 16 00 0 100 8 00 4670020 2 0 125 0 125 00 1 60 1 28 060 0 48 Clip Tip 200 62 50 1 60 256 0 60 0 96 12 50 1 60 1280 0 60 480 4670030 10 0 300 0 300 00 40 1 50 0 50 Cliplip 300 150 00 4 0 2 67 1 50 1 00 30 00 40 13 33 1 50 5 00 4670040 50 0 1250 0 1250 00 16 00 1 28 60 0 48 Clip Tip 1250 625 00 16 00 256 6 0 0 96 125 00 16 00 1280 6 0 4 80 E1 ClipTip Multichannel models Cat No Channels Range Volume Inaccuracy Imprecision ClipTip pl pl std GV 46 1000 8 ch 75 105 12 50 504007 320 0 200 1 60 Cliplip 12 5 0 25 0 400 640 0 200 3 20 175 0 400 3200 0 200 16 00 46 1040 8 ch 2 0 125 0 125 00 320 256 1 20 0 96 ClipTip 200 62 50 3 20 12 20 1 92 12 50 53 20 25 60 120 9 60 4671070 8 10 0 300 0 300 00 8 0 2 67 3 0 1 00 ClipTip 300 150 00 58 0 22 21 2 00 30 00 58 0 26 67 30 10 00 46 1100 8 50 0 1250 0 1250 00 3200 256 120 0 96 Ulm 1250 625 00 3200 4512 120 19 125 00 32 00 2560 120 9 60 46 1010 12 ch 05 125 12 50 50400 320 0 200 1 60 Cliplip 12 5 0 25 0 400 640 0 200 3 20 1 25 0 400 3200 0 200 16 00 4671050 12 ch 2 0 125 0 125 00 3 20 256 1 20 095 ClipTip 200 62 50 3 20 b12 120 1 92 12 50 53 20 25 60 1 20 9 60 4671080 12 ch 10
75. on settings and personalized pipette settings will be cleared The user is responsible for redefining personal changes after the recovery procedure D 3 2 8 Connect Piston he settings menu also contains the piston detachment connection mode for single channel pipettes 1 Select Settings and press WV Select 2 Select Connect piston and press V Select his mode allows the user to move the piston to a position where It Is easy to remove using the piston tool mode Is also used to connect the piston to the motor after service operations See section G 3 on how to remove and connect the piston Power function allows the user to manually shut down the pipette and save the battery when the pipette is not used for a longer period of time Thermo Fisher Scientific Sample Transfers Thermo Fisher Scientific To shut down the pipette 1 Select Power and press Select 2 Press 889 Yes to confirm the shut down Note he pipette will automatically go to power off mode after 1 h if not used D 5 Application Examples Examples of creating different pipetting sequences are given below Both Matrix and Preset can be used for the same tasks The preferred method is always described first Matrix Presets Note hese examples assume that the Matrix program is empty If not so then use the Delete all steps option in the Matrix edit view Example Fill and dispense 100 ul Using Presets Sel
76. ons for other liquids need to be done using the density value of the dispensed liquid and applicable formulas Thermo Fisher Scientific Thermo Fisher Scientific gt DE Note he user may lock the special calibration setting with a password so that the setting cannot be changed without authorization See section D 3 1 4 for more information Caution he user must make sure that the special calibration setting 15 suitable for the intended liquid transfer operation Failing to do so may cause serious dosing errors E 5 3 Editing Special Adjustments he stored special adjustments can be edited by the user Caution When changing a stored special adjustment the user created programs that have been defined to use the setting in question are affected 1 Select Settings and press Select 2 Select Calibrate and press 7 Select 3 oelect the adjustment setting to be edited from the list icon using a and press WV Edit 5 Proceed according to section E 5 2 from Step 4 onwards 4 Highlight the 6 Press SQ Done when the changes are ready 7 A confirmation window appears Accept with Yes 8 After applying a stored adjustment setting in the Programs mode section D 2 3 perform re calibration of the device according to section E 4 2 to verify the effect of new adjustment settings E 5 4 Deleting a Stored Adjustment Setting The stored special adjustment can be deleted by the user Cau
77. onse of the pipette Picture 044 D 3 2 1 Sounds The Sounds option allows the user to control the function indicator sounds and keypad key response sounds To modify the buzzer settings 1 Select Settings and press WV Select 2 Select Sounds and press WV Select 3 Scroll in the menu using A v 4 Set the pipetting and keypad sounds on off using gt 4 b sound level set at 5 different volumes 6 Press SQ Done after editing the settings D 3 2 2 Calibrate It is possible to reset the adjustment parameters for Default calibration or add and define new calibration settings special adjustment settings in the Calibrate function Default calibration values have been set at the factory quality control Changing the Default calibration parameters will affect all pipetting modes under the Matrix and Presets functions For more information on how to change Default calibration see section E 5 1 Add new calibration allows the user to define a special adjustment setting that can be used in stored programs It is possible to define up to five different adjustment settings For more information on how to define and use special adjustment settings see sections E 5 2 to E 5 4 D 3 2 3 Calibration Tracker The Calibration tracker function allows the user to set up a reminder that appears as an icon in the upper field of the pipette display The Calibration tracker informs the user when the set time limit o
78. ool end with a metal rod so that the screw end is facing upwards Place the O ring 7 into the rod above the tip fitting or make sure that the O ring is at the bottom of the screw den of the cylinder Replace O ring if necessary Align the tool with the cylinder 9 and carefully screw the tip fitting in by turning the tool clockwise Stop the tightening when you feel resistance Rotate the tip fitting tool and tighten the tip fitting lightly by using the end without metal rod Caution Be careful not to overtighten the the tip fitting This may damage the screw Joint in the pipette Note If the tip fitting is tightened using the end with the metal rod of tip fitting tool the tool starts to slip This may disform the tool and make it more difficult to use Single channel model 0 5 12 5yl with 384 format tip fitting 1 Remove the tip fitting by hand turning it counterclockwise Use the metal rod end of the tip fitting tool C to remove the O ring 7 from the cylinder den for inspection Replace the O ring if necessary 2 Place the tip fitting onto the tool end with a metal rod so that the screw end is upwards Place the O ring 7 into the rod above the tip fitting or make sure that the O ring is at the bottom of the screw den of the cylinder Replace O ring if necessary Align the tool with the cylinder 9 and carefully screw the tip fitting in by using two fingers until you feel resistance Remove the tool and tighten the tip fitting lightly by using t
79. p Fitting 11 ClipTip See section A 1 Spacing Set Buttons To set the tip spacing movement range within the scale See section B 5 2 Spacing Adjuster To change the space between the tips within the scale see section B 5 Color coded parts 14 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific Thermo Fisher Scientific B 2 Keypad and Operation Keys A Selection keys B Arrow keys C Tip ejection keys Picture B1 E1 ClipTip keypad A Selection keys he selection keys are used for activating modes and confirming selections B Arrow keys he Up and Down arrow keys are used to move up and down in the user interface he Left and Right arrow keys are used to edit settings such as volume and speed changes C Tip ejection keys The tip ejection keys are used to eject the tips from the pipette electronically There are two ejection keys to ensure convenient use for both right and left handed users When the pipette is ejecting tips the ejecting tips icon Is shown on the display he tip ejections keys are active only when the entire pipetting cycle 15 completed to prevent accidental tip ejection during pipetting he keys are always inactive during the pipetting cycle Thermo Scientific E1 ClipTip User Manual 15 15 E 16 Thermo Scientific E1 ClipTip User Manual D Trigger he index finger operated trigger is used to aspirate and dispense liquid It is al
80. password Picture 039 4 Set the password using the virtual keypad Picture 040 Press 99 Done when ready Note that the set password is the same for all protections that will be set 5 An info text confirming the password appears on the display Press W Ok 6 First select the main function name where the protectable item is located from the list Picture 041 and press Select 7 Select the item to be protected from the list and press Protect Picture D42 A a Icon appears on the item row to indicate that the item Is locked and cannot be edited before the password is entered or protection Is removed 8 Press 9 Back when all items to be locked are protected The Icon also appears the main function row that contains locked item Picture D40 Icon is black if all items under the main function protected gray if only certain items are locked under the main function Picture D43 9 Press SQ Back to exit the Password protection mode Password protection Password protection Password protection Set password Forward 1 Protect ack Back Picture D41 Picture D42 Picture D43 46 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific Service tracker Product Information Date and time Menu Picture D44 Thermo Fisher Scientific D 3 2 Settings The Settings function contains the tools to define basic functions tracking settings and audio resp
81. pette has to be recharged To extend the battery life cycle it is recommended to charge the pipette every two months even if the pipette is not used daily A typical E1 ClipTip battery lasts for around 300 charging cycles Warning An old battery can cause the pipette to malfunction and may cause a safety risk thermal runaway It is recommended that you change the battery every two years If the battery s recharging interval decreases significantly the battery should also be changed It is recommended to charge the pipette every two months even if the pipette is not used daily A 3 3 Using the Power Supply Insert the country specific power plug adapter into the power supply unit he power supply unit is marked with number 5 in the picture on the right Power plug adapters 1 Australia 2 Europe 3 United Kingdom 4 USA Japan Connect the lead of the charger to the socket on top of the display Then connect the charger to an AC wall socket You can use the pipette while the charger is connected Caution he chargers plug must be inserted in to a socket that Is easy to reach and from which it can be easily unplugged in case of emergency Warning Use only the original E1 ClipTip charger and battery pack The pipette battery may be empty when delivered and must be charged before initial use It is recommended to charge the battery for at least 2 5 h when charged for the first time Thermo Scientific E1 ClipTip User Manual 11
82. procedure must be verified by the user 0 5 Autoclaving Steam sterilization should be performed at 12120 252 F and 2 ata for 20 minutes Sterilization bags may be used if needed The parts are chemically resistant to the decontaminant The parts are not chemically resistant to the decontaminant Steam sterilization can be performed on the following parts With single channel pipettes e cylinder module e piston assembly e tip ejector assembly e tip fitting both 96 format and 384 format tip fitting and sealing rings 14 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific G 6 Disposal of A Materials Thermo Fisher Scientific Caution he cylinder should be kept inside the tip ejector assembly during autoclaving The tip fitting should be kept detached A JA eU With multichannel pipettes e tip fittings only 96 format tip fittings and sealing rings Other parts cannot be steam sterilized Caution user must verify the effectiveness of the autoclaving After autoclaving the tip cone and tip fitting must be cooled to room temperature for at least two hours before assembly and any operations Before pipetting make sure that the tip cone and tip fitting are dry G 6 1 Disposal of the Materials Follow laboratory and country specific procedures for the disposal of biohazardous or radioactive waste Refer to local regulations for the disposal of infectious ma
83. r a pipetting cycle limit is reached and reminds the user to recheck the pipette performance Thermo Scientific E1 ClipTip User Manual 47 48 Thermo Scientific E1 ClipTip User Manual To set the Calibration tracker 1 Select Settings and press Select 2 Select Calibration tracker and press Select 3 ecroll in the menu using A v 4 Set the remainder on off using 5 d 5 oelect the cycle based pipetting cycles or time based tracker type ge 5 6 Scroll to Reminder interval Pressing D a activates the Reminder interval window Set the limits using GY anc gt J for the selected tracker type he cycle based tracker can be set between 1 and 999999 pipetting cycles he time based tracker can be set between 1 and 999 days The Calibration tracker window also shows the cumulative values from the last calibration cycles when the cycle based option has been selected and days when the time based option has been selected and the date of the last calibration These values are reset when the default calibration is changed section E 5 1 Caution The Calibration counter notifies the user only about the limits reached according to the Default calibration The user is responsible for checking the validity of personalized adjustment settings used in stored programs D 3 2 4 Service Tracker This mode allows the user to set up the service tracker reminder parameters he tracker will show a reminder icon in the upper
84. r displacement ss principle Its intended use is for aspirating and dispensing K liquids The E1 ClipTip operates only with Thermo Scientific V ClipTip pipette tips Notice the difference with the E1 ClipTip Electronic Pipette Ideal experience even for complex protocols When combined with the secure interlocking ClipTip technology the electronic pipetting action and flexible user interface the E1 ClipTip Pipetting System provides a premium pipetting experience for even the most complex protocols 2 Electronic operation helps you enjoy pipetting again The E1 ClipTip Pipette combines interlocking ClipTip technology with electronic tip ejection and index finger pipetting action helping to reduce pipetting tip attachment and ejection forces This makes pipetting a more comfortable safe and enjoyable experience Perfect fit for sample transfers between different lab formats One pipette the E1 ClipTip Equalizer allows you to transfer samples between a variety of lab formats where only traditional single channel pipettes would have been suitable in the past Adaptable tip spacing allows you to set the distance between tips simply by sliding the scale to expand and contract to the desired setting This means fewer repetitions for multiple applications Ideal operation for multiple users and environments The E1 ClipTip provides simple and fast operation with increased user flexibility Users have complete control
85. ration KeyS _ _ _ _ E Rotating Display 17 B 4 Cliplip Interface and Electrical Tip 17 B 4 1 Clip lip 96 format lips 17 B 4 2 384 384 format Tips 17 B 4 3 lip Attachment 18 Adiustable ME e otra oe do ER PU qaqaqa Cid REGE pda Pesos 18 B 5 1 To Set the Stop Buttons to Match the Application 19 B 5 2 Use of the Adjustable Tip Spacing 20 C RECOMMENDATIONS FOR ACCURATE PIPETTING 21 D PROGRAMMING AND OPERATION 23 D 1 User Interface Overview 23 D 1 1 Introduction to Pipetting View 25 0 1 2 Volume Adjustment 26 D 1 3 Pipetting Speed Adjustment 2 28 42544 fen aves 28 1 22 Presei PUDICUOTI uU u ated sasa tind alah ede ose a ma wa sk qg q d des irm res ar 97 D22 UC 32 D 2 2 2 Stepper Multi dispensing 33 WEN eae oe EE chee
86. released for delivery have been manufactured and inspected according to our approved documents and procedures During inspection pipettes have fulfilled the Thermo Fisher Scientific s performance specifications which conform to 50 8655 standards Before released for delivery all pipettes are factory calibrated using purified water under conditions given in the calibration report and adjusted to give the volumes as specified Calibration balances are regularly serviced and checked with weights traceable to national and international standards As a mark of this a calibration report covering the test results is delivered together with the pipette Thermo Fisher Scientific Oy s operation system is certified by a third party auditor and conforms to the quality system standard ISO 9001 and environmental system standard ISO 14001 WARNING MOST COUNTRIES PROHIBIT THE SHIPMENT OF MATERIALS CONTAMINATED BY RADIOACTIVITY OR HAZARDOUS ORGANIC OR CHEMICAL COMPOUNDS WITHOUT A PERMIT ALL PIPETTES MUST BE PROPERLY DECONTAMINATED BEFORE THEY ARE RETURNED Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 79 J TECHNICAL SERVICE In addition to normal maintenance it Is recommended to service the instrument regularly at least every 12 months by the manufacturers trained service engineers his ensures that the product is properly maintained and provides trouble free functionality Contact the Thermo Fisher Scientific technical service departmen
87. s functions Caution Ensure that the charge level of the battery is high enough especially when using a longer pipetting sequence in the Matrix function Thermo Scientific E1 ClipTip User Manual 31 Menu Picture D13 Forward Dispense 300 0pl Picture D14 32 Thermo Scientific E1 ClipTip User Manual D 2 2 Presets Function The Presets function is designed for fast and easy pipetting without saving The last used parameters will be saved automatically The following pipetting modes are included Forward stepper Reverse Repetitive Dilute Pipette Mix Dilute Mix Manual When selecting Presets a list of preset pipetting functions will appear on the display Picture D13 Select the function by using A v By pressing Use you enter the view where you can start using the selected dispensing function D 2 2 1 Forward The forward mode is recommended for aquaeous solutions such as buffers diluted acids or alkalis To use the mode 1 Select Presets and press 7 Select 2 Select Forward from the list and press 889 Use The pipetting mode view appears on the display Picture D14 3 Place the tip s under the liquid surface and press the trigger to aspirate the liquid 4 Press the trigger to dispense the liquid Thermo Fisher Scientific Forward Counter Volume editor Done Picture D15 Ste Volume 10 0ul Picture D16 Thermo Fisher Scientific
88. ser Manual 77 Materials E1 ClipTip Pipettes Material Component External surfaces of the upper parts PA PMMA ABS polyamide polymethyl methacrylate acrylonitrile butadiene styrene Exterior and interior of the lower parts PEI PA PVDF EPDM FVM oteel POM PEEK PVC PP silicone Brass ClipTip Tips Tip Filters 18 Thermo Scientific E1 ClipTip User Manual PP Fe polyetherimide polyamide polyvinyldenfluoride ethylene propylene diene rubber fluorosilicone rubber stainless steel polyoxymethylene polyether ether ketone polyvinyl chloride polypropylene polypropylene polyethylene Thermo Fisher Scientific WARRANTY Thermo Scientific E1 ClipTip Electronic Pipette Warranty Certificate All Thermo Scientific E1 ClipTip M electronic pipettes are guaranteed to be free of defects in material and workmanship for a period of two years from the date of purchase Register Online at www thermoscientific com pipettewarranty Thermo Fisher Scientific will replace or repair the defective product upon prompt notification in compliance with the following conditions he warranty against defects in workmanship or materials will be honored as long as the product was used in compliance with the instructions for use and care he warranty does not apply to products exposed to physical or chemical abuse The Warranty is subject to the following conditions 1 A 2 year warranty applies for pipettes that have
89. sible for the validation of the system to enable production of reliable and safe results The performance related cautions in Chapter E CALIBRATION AND ADJUSTMENT should be checked If the pipetting performance is critical for the outcome of the specific application the result has to be assured with an alternative test and if this Is not an option by duplicate testing The possibility of an incorrect volume delivery during pipetting cannot be completely mitigated This device is to be used by trained personnel that have adequate laboratory skills Use of the device for pipetting any liquid to be injected into the human body is not permitted A 2 2 Safety Symbols and Markings hese symbols are intended to draw your attention to particularly important pieces of information and alert you to the presence of hazards as indicated Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Warning Risk of injury to the user s Warning Risk of electric shock Warning Biohazard risk WEEE symbol his product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2012 19 EC Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest Thermo Fisher Scientific Thermo Fisher Scientific PP P Pb b bP gt gt gt A 2 3 Safety Precautions he following safe
90. so used to initiate mixing The trigger and finger rest can be rotated 15 degrees in both directions to ensure an optimal pipetting position Dispensing into air 1 Press the trigger shortly to dispense the liquid Picture B2 2 he pipette returns to the ready position Note When using small volumes and certain liquid types it is recommended to keep the trigger pressed until all liquid has been dispensed from the tip Note After dispensing it is recommended to touch the tip s against the edge of the reservoir to remove possible remaining liquid on the outside surface of the tip Dispensing into liquid 1 Putthe tip into the liquid Picture B3 2 Press the trigger to dispense the liquid and keep the trigger pressed until the tip is withdrawn from the liquid 3 Lift the tip from the liquid and release the trigger 4 The pipette returns to the ready position Picture B2 Picture B3 Thermo Fisher Scientific B 3 Rotating Display B 4 ClipTip Interface and Electrical Tip Ejection Picture B5 ClipTip 96 format Tips x Picture B6 N ClipTip 384 Thermo Fisher Scientific The E1 ClipTip features a rotating full color display ensuring optimal visibility in different working environments The display rotates 30 degrees in both directions from the center position The rotating angle can be adjusted by turning the display left or right Picture B4 Rotating display The ClipTip technology is based on
91. stand the charging stand Battery not installed Install the battery following the instructions in section Getting Started Note Do not perform troubleshooting procedures on the internal components unless instructed by Thermo Fisher Scientific Technical Service personnel Warning f the corrective actions do not solve the problem do not use the pipette Contact service Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 65 Display Notifications Display information Battery power lost press Ok to set date and time Invalid date Press Ok Piston position error Press trigger to empty tips Piston position error Piston adjustment error Battery failure 1 Battery failure 2 66 Thermo Scientific E1 ClipTip User Manual Probable cause Battery power was lost or battery was empty Given date is not up to date Piston movement was not accurate during aspirating or dispensing due to mechanical friction or blockage Pipette is not serviced for a while and mechanical friction prevents accurate operation Pipette is not serviced for a while and mechanical friction prevents reliable calibration of the pipette Battery Is not connected Battery might be worn out outdated or damaged battery voltage was too low for charging Battery might be worn out outdated or damaged battery failed to charge The user interface of the E1 ClipTip electronic pipette d
92. t for more details See the list below for contact information J 1 Packing for When sending the pipette for service follow the guidelines presented below Service Warning Health risk from contaminated device It is important that the instrument is thoroughly decontaminated before it is removed from the A laboratory or any servicing is performed on it Guidelines for decontamination can be found in Chapter G MAINTENANCE When you ship the instrument for service remember to e nform about the use of hazardous materials e Use the original packaging to ensure that no damage will occur to the Instrument during shipping Any damage will incur additional labor charges e Enclose a dated and signed decontamination declaration request from your service provider inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return goods authorization number RGA given by your Thermo Fisher Scientific representative e Indicate the fault after you have contacted your local Thermo Fisher Scientific representative or Thermo Fisher Scientific s technical service department Refer to Chapter H TECHNICAL DATA for details on storage temperatures 80 Thermo Scientific E1 ClipTip User Manual Thermo Fisher Scientific J 2 Contact Information Thermo Fisher Scientific Technical Product Support For technical product support contact your local Thermo Fisher Scientific dealer or
93. t setting for the pre step is In use but It can be changed to Not in use Scroll to Pre step and change the setting using D d Picture 016 7 Press Done when the changes are ready Thermo Scientific E1 ClipTip User Manual 33 Picture D17 34 Thermo Scientific E1 ClipTip User Manual D 2 2 3 Reverse The Reverse mode reverse pipetting is recommended for viscous and volatile liquids It is also recommended for solutions with low surface tension e g liquids with foaming tendency In this mode an excess amount of liquid Is aspirated in addition to set the volume This mode is also useful when performing reagent addition where no purge is desired when dispensing To use the mode 1 Select Presets and press WV Select 2 Select Reverse from the list and press 7 Use 3 Place the tip s under the liquid surface and press the trigger to aspirate the liquid 4 Press the trigger to dispense the liquid An excess volume will remain Inside the tip 5 The display shows Purge Picture D17 Press the trigger to discard the excess volume To change the settings 1 Press 7 Edit in the pipetting mode view 2 Change the volume using gt 5 the Volume editor section 0 1 2 3 Scroll to Speed in and change the value using gt 2 4 Scrollto Speed and change the value using gt 4 5 Scroll to Counter and select Counter off Counter on using gt d 6 Press WP Done when t
94. t volume can be set between the minimum and maximum volumes of the pipette model Picture D29 3 Scroll to Speed in and change the value using gt 2 4 Scroll to Speed and change the value using gt a D Press SQ Done when the changes are ready D 2 3 Programs Function Application he Programs function is designed for creating and storing programs Save time and ensure data integrity by storing your most used protocols Description he Programs function includes both Matrix and Presets based programming styles See the chart in section D 1 It is possible to store up to 20 individual programs with personalized names and with liquid or application specific calibrations adjustment settings for increased accuracy and precision See sections E 5 2 and E 5 3 for further information Adding a new program 1 Select Program and press Select 2 Scroll to Add new program and press 7 Add to add a new program Picture 030 3 The following programs can be selected by scrolling and pressing WV Select Matrix Forward Stepper Reverse Repetitive Dilute Pipette Mix Dilute Mix and Manual Thermo Scientific E1 ClipTip User Manual 41 01 Forward 1 Calibration Default calibration Edit name Done Picture D31 Programs H Add new program Forward Menu Picture D32 42 Thermo Scientific E1 ClipTip User Manual 4 The new program is given a default name that can
95. te e Infectious waste Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative The pollution degree describes the amount of conductive pollution present In the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation The spare parts available for each pipette model can be found in Appendix 4 Contact your local Thermo Fisher Scientific dealer for part orders Thermo Fisher Scientific Thermo Fisher Scientific H TECHNICAL DATA Battery Type Capacity Charging time Charging temperature Power supply unit Type Input voltage Output voltage Display Type SI Resolution E1 ClipTip pipette Weight with battery tip not included Operating temperature Air humidity during operation otorage temperature Li ion battery 1280 mAh Approx 2 h 0 to 40 C Switching power supply 100 240 V 50 60 Hz 400 mA 5 0 V 600 mA max Color TFT LCD 22 1 6 x 220 pixels single channel pipette 190 g max Multichannel pipette 320 g max 15 to 35 C 20 to 85 rel humidity lt 1 month 20 to 45 C gt 1 month 10 to 35 C It is recommended to charge the pipette every two months even if the pipette is not used Thermo Scientific E1 ClipTip U
96. ten Phone 31 204 877 030 Email unity nlQthermofisher com Spain Thermo Fisher Scientific Unity Lab Services C Luis 1 No 9 28031 Madrid Phone 34 91 380 67 33 Email unity es thermofisher com Sweden Thermo Fisher Scientific Unity Lab Services S dra L ngebergsgatan 30 V stra Fr lunda SE 421 32 Phone 46 31 352 00 Email unity se thermofisher com Thermo Fisher Scientific Switzerland Thermo Hisher Scientific Unity Lab Services Keltenweg 35 4460 Gelterkinden Phone 41 56 618 41 21 Email unity ch thermofisher com United Kingdom Thermo Hisher Scientific Attn Pipette Service Lab Bishop Meadow Road Loughborough Leicestershire LE11 5RG Phone 44 0 1509 555 494 Email unity uk thermofisher com United States Thermo Fisher Scientific Unity Lab Services Attn Pipette Service Lab 2200 Friars Drive Hudson NH 03051 Phone 800 345 0206 Email matrix technicalservice thermofisher com If your country is not listed refer to the Unity Lab Services web page http www unitylabservices com or contact your local Thermo Fisher Scientific dealer to receive contact information to your closest authorized Thermo Fisher Scientific pipette Service Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 83 Appendix 1 Conversion Table Value of the conversion factor Z ul mg as a function of temperature and pressure for distilled water Temperature Air pressure G
97. ter off Counter on using gt 2 6 Press SQ Done when the changes are ready D 2 2 5 Dilute he dilute mode enables the user to dispense two selected volumes with an air gap in between This is useful for instance when making standard curves To use the mode 1 Select Presets and press WV Select 2 Select Dilute from the list and press Use 3 Place the tip s under the surface of liquid 1 and press the trigger to aspirate the liquid fill 1 4 Remove the tips from the liquid The display shows Air gap Picture D19 5 Press the trigger to aspirate an air gap 6 Place the tip s under the surface of liquid 2 and press the trigger to aspirate the liquid fill 2 Press the trigger to dispense both volumes Thermo Scientific E1 ClipTip User Manual 35 Dilute Volume based Fill 1 volume 285 0yl Fill 2 volume 15 0ul Fill 1 speed 10 Picture 020 Dilution type Ratio based Total volume 300 0 Picture 021 36 Thermo Scientific E1 ClipTip User Manual To change the settings The volumes used in the Dilute mode can be set in two different ways ratio based and volume based he ratio based method uses different dilution ratios to determine the volumes to be used Total volume and the dilution ratio define the diluent fill 1 and sample fill 2 volumes automatically The diluent fill 1 is the larger of the volumes In the volume based method t
98. terial Warning he tips can be potentially infectious Dispose of all used disposable tips as biohazardous waste Note he pipette should be regularly serviced according to the User Manual he pipette calibration and functioning must be checked after maintenance Use only original Thermo Scientific spare parts and ClipTips Avoid excess grease Use only the grease provided with the pipette Thermo Scientific E1 ClipTip User Manual 75 G 7 Spare Parts 16 Thermo Scientific E1 ClipTip User Manual The pipette should be checked at the beginning of each day Particular attention should be paid to the tip cone It is recommended to 7096 ethanol for wiping the pipette If the pipette is used daily it is recommended to service the pipette every three months G 6 2 Disposal of the Pipette Follow the guidelines below for the disposal of the E1 ClipTip pipette Warning Decontaminate the pipette before disposal Refer to section G 4 Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Warning he used lithium Li ion battery is regulated waste and must be disposed of according to local regulations Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste procedures vary by country Pollution degree 2 according to IEC 60664 1 Method of disposal e Electronic waste e Contaminated was
99. there is a need for aligning the tips to the vessel or tip rack etc 1 Depress the stop buttons while moving them to the outmost positions 2 Attach the tips 3 Slide the tips into the desired minimum position using the Spacing adjuster 4 Depress the Stop button minimum and move it to the edge of the Spacing adjuster 5 Release the stop button to lock the positions for use 6 Slide the tips into the desired maximum position using the Spacing adjuster 7 Depress the Stop button maximum and move It to the other edge of the Spacing adjuster 8 Release the stop button to lock the positions for use B 5 2 Use of the Adjustable Tip Spacing The following instructions are examples of how to use the adjustable tip spacing Note that the amount and sequence of used tip spacing positions can vary according to the requirements of the application Note Always use the minimum tip spacing position when attaching tips from a rack stop button positions are ready set according to the instructions section 5 1 1 Slide the Spacing adjuster to the edge of the Stop button minimum 2 Attach the tips from a rack 3 Slide the Spacing adjuster to the edge of the Stop button maximum 4 Press the pipetting trigger to fill the tips with liquid for example from Microcentrifuge tubes Picture B7 5 Slide the Spacing adjuster to the edge of the Stop button minimum 6 Press the pipetting trigger to dispense the liquid for exampl
100. tion When deleting a special adjustment the user created programs that have been defined to use the setting in question are affected The programs that have been defined to use the deleted adjustment will automatically be changed to use the Default calibration adjustment setting A notification of the adjustment setting change will be displayed when the linked program is used the next time The notification will only appear once 1 Select Settings and press Select 2 Select Calibrate and press 7 Select 3 Select the adjustment setting to be removed 4 Highlight the W icon using D and d and press Delete 5 A confirmation window appears Accept with W Yes Thermo Scientific E1 ClipTip User Manual 63 Defect Leakage Inaccurate dispensing Tip not ejecting No dispensing F TROUBLESHOOTING he table below lists possible symptoms and their solutions Possible reason Tip fitting incorrectly attached or loose ClipTip clips are bent Tip fitting O ring damaged Tip incorrectly attached Foreign particles between the tip and the tip cone Insufficient amount of grease on the cylinder and the O ring or foreign particles between the piston the O ring and the cylinder Incorrect operation Tip incorrectly attached Unsuitable calibration Wrong tip Tip fitting incorrectly attached or loose Tip fitting incorrectly attached or loose Battery too low Battery too low Pistons stuck Piston not
101. to set personalized functions for their applications and utilize optional password protection This is ideal for multi user environments so you can avoid unnecessary editing and scrolling through submenus Pipette for multiple applications Choose from single channels 8 16 channel multichannels or 6 12 channel adjustable tip spacing multichannel pipettes within the volume range of 0 5 1250 ul 384 format E1 ClipTip pipettes are ideal for 384 microplate applications Flexible re charging You can decide whether to use either a charging stand or a plug Each E1 ClipTip pipette is shipped with a universal wall power plug The charging stand allows safe storage while ensuring that the E1 ClipTip is always charged when you need it Two year extended warranty The E1 ClipTip pipette is guarenteed to be free of defects in material and workmanship for a period of two years from the date of purchase A two year warranty applies for pipettes that have been registered while one year warranty to non registered ones Visit www thermoscientific com pipettewarranty to register the pipette and for more information Utilized in all 96 format E1 ClipTip pipettes except 0 5 12 5 ul Thermo Fisher Scientific Thermo Scientific E1 ClipTip User Manual 5 Using this Manual Read the User Manual before using the device pipette and tip for the first time Please check the User Manual if needed Package E1 ClipTip Pipette Universal charger
102. ty precautions provide important information intended to prevent personal injury to the operator and or others and damage to the E1 ClipTip pipette Warning Follow general instructions for hazard prevention and safety Instructions e g wear protective clothing eye protection and gloves Warning Follow the ergonomic guidelines for laboratory work to minimize the risk of repetitive strain injury RSI e g the Good Laboratory Pipetting Guide available at www thermoscientific com glp Warning Follow the safety instructions and general laboratory practice for use and waste disposal of hazardous material e g radioactive and potentially infectious Warning Ensure that the power transformer is connected to a power receptacle that provides the voltage and current specified in this user manual Use of an incompatible power receptacle can cause shock and fire hazard Warning Follow the E1 ClipTip pipette instructions presented in this user manual in Chapter H TECHNICAL DATA Failure to follow the instructions may cause a safety risk Warning Do not immerse the unit in liquid and do not clean the upper part of the pipette by spraying Fluid seepage can damage internal components and may cause a safety risk Warning Incorrect handling of the battery or pipette may cause personal injury Do not drop the battery Do not expose the battery to any kind of mechanical stress or extreme temperatures above 60 C 45 C when charging or under 0
103. umbers for the seconds Each number can be selected separately Use v to increase the time and to decrease the volume Switch to the next number by using e 9 Press Done when the time has been changed Note text a note text e g a reminder can be added To add a text see section D 3 1 1 The maximum length of the text is one row Press WP Done when the text is ready Tip eject 2 ejection of the tips A tip ejection step can be selected in the custom program when there Is no more liquid in the tip Note f no purge step has been added to the pipetting program then the text A purge step will be added will appear on the screen Press Vf Ok Note f the volume in a step makes the total dispense volume greater than the total fill volume before that step then an exclamation mark will appear in front of the step to indicate that the pipetting sequence has not been correctly created he note text Invalid program Edit steps will appear upon completion of the pressing stage he pipetting sequence has to be edited before use Is possible 4 Fill 10 0 U Dispense 300 01 Note If no runnable steps have been defined to the mode the note text Program does not contain any steps will appear Edit the program to add steps Press Edit to add steps or SQ Cancel to return to the main menu Caution he user is responsible for the validity of the created pipetting sequence in Matrix and Program
104. wo fingers Caution The user must make sure that the small tip fitting O ring 7 is in place before attaching the tip fitting The tip fitting tool or a disposable tip can be used to place the O ring into place Failing to check this may cause leakage of the pipette Note The product package contains spare part sealing rings Store them for future maintenance purposes Multichannel models with 384 format tip fitting he multichannel pipette models with 384 format tip fitting cannot be disassembled by the user See Chapter J TECHNICAL SERVICE for more information to service your pipette Thermo Fisher Scientific G 3 Disassembly and Assembly i Picture G4 Connect piston Keep trigger pressed to drive piston down Picture 15 Thermo Fisher Scientific G 3 1 Single Channel Pipettes Disassembly 1 Remove the tip fitting 5 as described in section G 2 2 Remove the tip ejector 8 by rotating it counterclockwise 3 Remove the cylinder 9 using a corresponding slot in the tool A Turn clockwise to open the screw joint and pull out the cylinder module Picture G4 Use the tool end 1 for models 12 5ul 125ul and 1250 1 and the tool end 2 for model 3001 4 Select Z Settings and press Select in the main menu of the pipette 5 Select Connect piston from the list and press 8 Select Picture G5 6 Press and hold the trigger down to drive out the piston 7 Insert the piston tool B and pull out the p
105. x 384 rack 10 x 384 rack 10 x 384 rack 10 x 384 rack 10 x 384 rack ClipTip Reloads non sterile and sterile Order number 94410217 94410218 94410257 94410258 94410317 94410318 94410517 94410518 94410717 94410718 94410718 94410818 Description ClipTip 20 Reload Stack ClipTip 20 Reload Stack sterile ClipTip 50 Reload Stack ClipTip 50 Reload Stack sterile ClipTip 200 Reload Stack ClipTip 200 Reload Stack sterile ClipTip 300 Reload Stack ClipTip 300 Reload Stack sterile ClipTip 1000 Reload Stack ClipTip 1000 Reload Stack sterile ClipTip 1250 Reload Blister ClipTip 1250 Reload Blister sterile 94 Thermo Scientific E1 ClipTip User Manual Volume range 1 20 ul 1 20 ul 5 50 ul 5 50 ul 5 200 ul 5 200 ul 10 300 ul 10 300 ul 30 1000 ul 30 1000 ul 50 1250 ul 50 1250 ul Color Coding Pink Pink Violet Violet Yellow Yellow Orange Orange Blue Blue Turquoise Turquoise Oty 10 x 96 insert 10 x 96 insert 10 x 96 insert 10 x 96 insert 10 x 96 insert 10 x 96 insert 10 x 96 insert 10 x 96 insert 8 x 96 insert 8 x 96 insert 8 x 96 insert 8 x 96 insert Thermo Fisher Scientific ClipTip Filter tips Order number 94420043 94420213 94420253 94420313 94420513 94420713 94420813 384 ClipTip Tips 94420053 94420103 94420153 Description ClipTip Filter 12 5 sterile ClipTip Filter 20 sterile ClipTip Filter 50 sterile ClipTip Filter 200 sterile ClipTip Filter 300 steril

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