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Forma Steri-Cult CO2 Incubator User Manual

Contents

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2. FILTER G 127100 SPACER M F 4 40 X 1 75 L W 125035 5 16 x 3 8 NYLON SPACER H 190222 SPACER SOLENOID VALVE X 600034 3 8 SNAPPER HOSE CLAMP I 220313 FUSE LABEL 3 4 AMP Y 730044 NYLON SHOULDER WASHER J 220358 FUSE LABEL 1 2 840020 1 4 BULKHEAD FITTING K 220378 FUSE LABEL 4 5 AA L 220477 GROUND SYMBOL LABEL 5 0 BB 600034 SNAPPER HOSE CLAMP M 230105 FUSEBLOCK 5 X 20MM cc 840045 1 4 HOSE X 10 32 ADAPTER N 230120 FUSE 500MA 5 X 20MM DD 290128 1 2 SS INT TOOTH LK WASHER 230135 FUSE 1 TD 5 X 20MM 117000 1 8 NYLON PUSH IN PLUG P 230201 FUSE 750MA 5 X 20MM T STAT AND BACK RIGHT AND TOP HEATER LEADS LEFT BOTTOM AND 0002 HEATER LEADS MAIN CABINET WIRING FRAME HEATER LEADS 270108 FERRITE 290185 RH CONDITIONER PROBE HUMIDIFIER CONNECTION PLENUM HEATER LEADS ULTRA SONIC HARNESS 1900145 HUMIDITY VALVI 250128 n NIE CIRCUIT BREAKER 3307 3310 154 230196 3308 3311 10A 230179 ly ETT Ig 0 2 1 430337 RIBBON CABLE FROM MICRO BOARD R YES NES INNER 0002 1900146 SWITCH 360263 COMAN D UTER 0009 SWITCH 360251 COWN D WATER LEVEL SWITCH 360171 NOTE WATER LEVEL SWITCH IS MOUNTED IN H20 BOTTLE ASSY L CABLE TO BLUE ADD WATER INDICATOR H20 FILL VALVE 2500
3. 4 7 Setting RS485 Communications Address 4 8 Section b Alarms deducant mad ati us Ns 5 1 Temp Controller Failure TEMP CNTRL ERROR 5 2 RH System Fault uid shani 5 2 Sensor Alarms n asa en tenes 5 2 Gis Guard Alaris cus ta aaa beet ava os ae 5 2 Water Level Alarms Eau 5 3 Door Open 1 gait eant 5 3 Section6 6 6 1 Section7 Routine Maintenance 7 1 Cleaning the Incubator Interior 7 1 Cleaning Humidity System 7 2 Cleaning Cabinet Exterior 7 2 Flushing the Humidity System 7 4 HEPA Filter Maintenance 7 6 Section8 Sterilization 8 1 vil 7023307 Steri Cult Thermo Scientific Table of Contents Section9 Factory Installed Options 9 1 Remote Alarm Contacts 9 1 RS485 Interface Rd REA od 9 2 Connecting the Analog Output Boards 9 3 Gas Guard zoe tended 9 5
4. si UNOA y AjejeuurxoJdde 158JJ y os Buiddius siu INO peddius si ejep SUJUOW suejs pouag 941 xALNVSSIVM YOLVENONI LIND 1431 TIVNOLLVNS3ANI 9131133195 43 13 picofast Snap Lock Connector Instructions UNLOCK P menen m With TURCK s patented Locking Sleeve pulled back any picofast sensor slides on without any difficulty lt After sensor is connected push Locking Sleeve for ward to create a watertight connection It s a snap Installation Instructions SS 1 Do not twist 1 sure black locking sleeve is pulled back 2 Line up pins and push BM connector onto plug 2 Pull locking sleeve back You will feel a snap to unlocked position Do not twist r 3 Push locking sleeve forward on connector until it is flush with the front of the connector 3 Pull connector straight off 4 If sleeve is difficult to slide on a new connector 4 Leave locking sleeve in exercise it a few times unlocked position Do not use tools ra H7 TURCK Inc 3000 Campus Drive Minneapolis MN 55441 Application Support 1 800 544 PROX Fax 763 553 0708
5. Stainless steel perforated Clearance Adjustable 1 160 centers Dimensions 3307 3308 17 7 x 19 9 45 2cm x 50 5cm Surface 2 4 sq ft per shelf Dimensions 3310 3311 25 7 x 19 9 65 5cm x 50 5 Surface area 3 6 sq ft per shelf Loading 50 lbs 22 7kg stationary Sterilization Cycle Setpoint quadr Sie eee eae Sa d 140 C Cycle time Approximately 14 hours 23 C nominal line voltage Activation Digital touch pad Construction Interior volume 3307 3308 8 2 cu ft 232 2 liters 3310 3311 11 4 ft 322 8 liters Interior 304 stainless steel mirror finish coved corners Exterior 18 gauge cold rolled steel powder coated Outer door gasket Four sided molded magnetic vinyl Inner door gasket Silicone feather Insulation Mineral wool Fittings Access port1 4 3 6cm with removable silicone plug 2 1 4 0 64cm hose barbed Drain 3 8 0 95cm barbed fitting with shutoff 10 2 7023307 Steri Cult Thermo Scientific Thermo Scientific Section 10 Specifications Electrical 115V models 115VAC 50 60 Hz 1PH Operating range 90 125VAC Model 3307 10 5 FLA Model 3310 11 5 FLA Circuit breaker power switch 15A 2 Pole Model 3307 3310 230V models 230VAC 50 60 Hz 1 PH Operating Range 180 250
6. 2 20 reo 37 22 ORANGE 62 20 ORANGE 85 Gv 38 22 TDRANE 63 20 DRANGE 4 20 22 6 20 BLACK 86 5 20 reo 40 22 YELDy 65 20 41 22 66 87 7 20 42 22 reo 8 2 mak 88 4 22 mak 6 20 18 45 22 WHITE 70 89 21 18 GREEN 46 22 BN 7 20 BROWN 22 18 47 22 72 20 90 23 18 48 22 73 20 ORANGE gl 7A 20 BROWN xa HEATER ELEMENT STUCK NUMBER AND WATT VALUE 93 MODEL 3308 HEATER stock NORMAL 5 5 STERI 5 5 94 POSITION No warts DHMS WATIS UMS DHMS 8 LEFT 5 132106 12 3 4085 77 4515 85 84 3 596 14 658 90 95 RIGHT SIDE 132107 14 6 3442 12 3804 45 82 2 611 37 675 73 BACK 32110 20 3 2475 62 2736 21 99 9 503 05 556 01 96 g BOTTOM 32108 122 4119 26 4552 87 121 7 412 94 456 41 Q7 FRAME 32104 628 800 24 884 47 62 8 800 24 884 47 DIDP 32109 782 642 65 71029 386 7 129 95 143 64 98 RH COND 132111 276 1820 83 2012 50 MA B PLENUM 32112 291 1726 98 1808 76 175 3 286 68 316 85 100 HEATER ELEMENT STOCK NUMBER AND WATT VALUE 101 MODEL 331 102 HEATER STOCK NORMAL 5 5 STERI 5 15 POSITION No warts pis warts
7. 2 1 Setting the Overtemp Setpoint 2 2 Setting the CO2 Setpoint 2 3 Setting the Relative Humidity RH Setpoint 2 3 Calibration Ser da a a a 3 1 Calibrating the Temperature 3 1 Calibrating the Infrared CO2 System 3 2 Calibrating Relative Humidity RH 3 3 7023307 Steri Cult vi Table of Contents Section4 Configuration 4 1 Turning the Audible Alarm ON OFF 4 New HEPA Filtet 4 1 Setting the REPLACE HEPA Filter Reminder 4 2 Setting an Access Code 4 2 Setting a Low Temp Alarm Limit 4 3 Enabling Temp Alarms to Trip Contacts 4 3 Setting Low Alarm Limit 4 4 Setting a High CO2 Alarm 1 4 4 Enabling CO Alarms to Trip Contacts 4 5 Setting a Low RH Alarm Limit 4 5 Setting a High RH Alarm Limit 4 6 Enabling RH Alarms to Trip Contacts 4 6 Selecting a Primary 4 7 Disabling the Gas Guard System
8. 62 8 200 06 22112 62 8 200 06 221 12 DOOR 132099 78 2 160 66 177 57 386 7 32 49 35 91 100 RH COND 132101 27 6 455 21 503 13 WA PLENUM 132102 29 1 431 74 477 19 175 3 71 67 79 21 101 102 HEATER ELEMENT CHART STOCK NUMBER AND WATT VALLE 103 MODEL 3310 HEATER STOCK NORMAL 5 5 STERI 5 57 POSITION yrs UMS OHMS wATTS DHMS LEFT SIDE 132076 8 0 1570 47 1735 78 478 262 84 290 51 RIGHT SIDE 132077 12 4 1013 21 1119 86 70 8 177 45 196 13 32080 34 8 361 03 399 03 1627 77 22 85 35 32075 84 1495 68 1653 13 124 8 100 67 111 27 Borrow 32078 13 4 937 59 1036 29 142 2 88 35 97 65 FRAME 32074 64 3 195 39 215 96 64 3 195 39 215 96 DOOR 32079 74 2 169 32 18715 4417 28 44 31 44 RH COND 32101 27 6 455 21 503 13 PLENUM 32082 14 8 948 90 938 25 100 9 124 52 137 62 11 IN 4660 07 15 15 JAS SAG CCS 2440 CABLES WERE 2240 10 IN 4582 10 15 14 DAG SAG DRP 360283 WAS 360230 9 IN 4469 02 25 14 GLM SAG LUN 250139 WAS 250085 8 1 10076 10 08 08 GJG GJG LUN CHANGED FAN PART NUMBER 7 1 3242 03 07 05 LUN CORRECT RH SENSOR 290154 290174 6 3150 2 17 03 DCB LON CHANGED INNER DOOR SWITCH FROM 360251 REV NO DATE CAD DESCRIPTION OF REVISION E d
9. B38WIIN OKO 7023307 Steri Cult Thermo Scientific E s pann 0515 OIHO V113IHVM 679 8 S 0011058 191930 8 319NV 3115 ONIAVUO 031112345 3SIW83H10 53101 JONVYITOL 2 1 3126 Y N ATONISSY H2N3B 1183 1 Jeusiyouueur 05220 11 2 21516 190 044 WH 0 2 WH NAG 8000 55 19 YANNI 111218315 31111 OMG BOH NOUS 808 DH IN NOL RON INOHLIM 1504804 9 801 0350 YON 15048404 ANY 801 543510 Ol 035012510 38 OL LON 51 NOI IVNHOJNI HONS NV NOILIVHUOJNI Thermo Scientific Section 11 Servicing SYOLVENINI 110218315 8066 1066 3NYN 1394 1300 AYVLAIYdOYd SNIVINOD LNAWNDOG SIHL 5 SEOCI N d LGI ALY 390 INW 310H M 31 14 4000 55 19 S3NNI 1 91 2910061 21 8 YIHSVM 1V3S 18021115 00 2 980081 00 1077 X 01 882 3AVM 160081 01 3HSVM 1V14 SS 01 S82 960081 6 9817201 2010114 2 8 610019 8 395 41111 4 GH 1 8 1 1 X 26 08 550056 1 Miu2S 54111 1 4 GH 55041 v X 26 0 750056 9 0 0 26 11 832 45 80143 220521 5 1V130 4431 H9IH Vul 210021 v u3HSVM lt 10061 03A0N3U 522 201 7341 01 10 10 L86E NI v 1091 8000 10 9NIIN0ON N9IS303U ANG WH NW 2 91 21 89
10. Steri Cult Model 3307 and 3310 Series C02 Incubator Controlled RH with Sterilization Cycle Operating and Maintenance Manual 7023307 Rev 19 Visit us online to register your warranty Thermo www thermoscientific com labwarranty SCIENTIFIC Model Size Voltage 3307 Medium 115 3308 Medium 230 3310 Large 115 3311 Large 230 Preface MANUAL NUMBER 7023307 19 40270 1N 4660 7 20 15 Change remote alarm connector update schematics CCS 18 31343 IN 4592 10 22 14 New magnetic door switch exploded drawing electrical schematics RoHS CCS 17 29174 1 4469 3 21 14 Updated solenoid pgs 11 8 through 11 12 CCS 16 10 21 13 Replaced the typo pan with bottle on pgs 1 10 through 1 11 CCS 15 28727 6 18 12 Clarified nebulizer flush instructions pg 7 4 amp 7 5 CCS 14 28408 5 18 12 Chg d 9596 non condensing in Specifications to 9096 CCS 13 2 891 10 27 11 Clarified Water Empty alarm language on pg 5 3 CCS 12 27292 1 4073 9 28 11 400119 to 400201 switcher kit pg 11 1 CCS 11 27162 1 4071 3 24 11 Added pilasters and shelf channels to Parts Listccs 11 24 10 Corrected typo in chart above 3308 3310 medium large CCS Thermo Scientific 7023307 Steri Cult CAUTION Contains Parts and Assemblies 4 aA Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in t
11. 1 d d d 7 dud C UJ CO OD OUI A w N O CO CD KRY J7 1 30 oe J7 2 J7 3 R A J7 4 gt 33 7 5 3 T JI 6 455 J7 7 4 35 p JI 8 PEL J6 1 8 56 2 33 z oy 5 3 440 J6 4 RI ey 5 5 26 6 43 25 7 44 20 J5 8 45 lov 6 9 59 46 5 10 7 49 26 11 GND a 3033 J6 13 1 50 D con 6 15 L s lt zi NN s 5 45 3 0 n 53 4 Sr pA ef iiess 42 B27 uU 4l 6 5 RH 24 et of fl 23 cgs 485 4 2 jt 087 57 2 5 465 Er J8 1 58 59 8 2 J8 3 60 z N D Dene 28 4 8 5 82 28 6 48 7 8 8 9 m TEMP _ ANALOG CD2 e Jt OUTPUT X BOARD v 02 DOOR SWITCH INNER 360263 Shown w door open DOOR SWITCH OUTER Shown w door open 360251 WATER LEVEL SWITCH 360171 ULTRA SONIC FILL SWITCH PART OF HUMIDITY ASSEMBLY DDR TEMP PROBE 2252 OHM 25C CONTROL 290185 AIR TEMP RH CONDITIONER PROBE 2252 OHM 25C 290185 IR CO2 SYSTEM 231204 IR amp RH SENSOR DOOR DETECT 360283 2 SENSORS IN PLACE RH SENSOR 290174 REMOTE ALARM CONNECTIONS shown in alarn sta
12. 18 w 29 20 Back 54 2473 RED 8l sp 20 w 9 22 55 24 3 WHITE 6 20 3 22 56 24 2 RED 82 7 20 oRANGE 32 22 vz 57 242 BLACK 93 8 purple 33 22 vz 58 20 20 vetoy 34 22 mu 5 20 RED 84 20 5 22 6 20 BLUE I 20 22 URANG 61 20 BLUE 85 2 20 RED 37 22 oRANGE 62 20 ORANGE 18 38 22 63 20 ORANGE 4 20 RED 39 22 ORANGE 4 20 BLACK 5 20 RED 40 22 65 v 87 6 20 ORANGE 41 22 YELLOW 66 7 20 BuE 4 20 RED 8 88 8 43 22 Bak 44 22 BAK ova 89 20 18 RED 45 22 wate 70 21 te GREEN 46 22 7 QA 90 22 18 oRANGE 47 22 BRON 72 gl 23 18 GREEN 48 22 RED 73 20 ORANGE 74 20 BROWN g2 93 94 HEATER ELEMENT CHART STOCK NUMBER AND WATT VALLE MODEL 3307 95 HEATER STOCK NORMAL 5 5 STERI 5 57 POSITION No watts WATIS OHMS 0 96 8 LEFT SIDE 132096 12 3 1021 44 1128 96 84 3 149 04 164 72 RIGHT SIDE 132097 14 6 860 53 95111 822 152 84 168 93 97 BACK 132100 20 3 618 90 684 05 99 9 125 76 139 00 e 38 5 sory 132098 12 2 1029 82 1138 22 121 7 103 24 114 10 99 FRAME 132000
13. No Inner Door is Open DOOR IS OPEN 15 min 5 min No C02 gt 602 High Tracking Alarm C02 IS HIGH 0 min 5 min Prog 02 lt 002 Low Tracking Alarm C02 IS LOW 15 min 5 min Prog No water flow through the water valve NO WATER FLOW 5 min 5 min No TEMP TEMP Low Tracking Alarm TEMP IS LOW 15 min 5 min Prog RH RH Low Limit Alarm RH IS LOW 30 min 5 min Prog RH RH High Limit Alarm RH IS HIGH 0 min 5 min Prog RH System Fault CHECK RH SYSTEM 60 min 5 min No Change HEPA filter reminder set time expired CHANGE HEPA SOON Omin No RH Conditioner Failure RH COND HEAT ERR 60 min 15 min No is low switch to tank 2 Gas Guard only TANK 1 LOW 0 min N A No Tank is low switch to tank 1 Gas Guard only TANK 2 LOW 0 min N A No Tank 1 and 2 are low Gas Guard Only TANK 182 LOW 0 min 15 min No Water Bottle is Low WATER LOW 0 min N A No Visual only no audible alarm All alarm delays and ringback times 30 seconds When multiple alarm conditions occur active messages display in the message center one at a time updating at 5 second intervals Pressing Silence during multiple alarms causes all active alarms to be silenced and ringback in 15 minutes The temperature alarms are disabled when the temperature setpoint is 10 C The alarms are disabled when the CO set point is 0 0 RH alarms are disabled when the setpoint is 096 RH 7023307 Steri Cult 5 1 Section 5 Alarms Te
14. Operation of the Gas 9 5 De activating the Gas 9 5 Connecting the CO Gas Supplies 9 5 Dataloggger 1221212929 ERR LRL RR RE EU 9 6 Section 10 Specifications 10 1 Section 11 Servicing 11 1 Parts upa 11 1 Replacing the Fuses 11 3 Replacing the and RH Air 11 3 Exploded Parts Drawings 11 5 Winnog Diagrams 13 ws DX eee RN 11 8 Electrical Schematics 11 10 Thermo Scientific 7023307 Steri Cult viii Section 1 Installation and Start Up Power On Off Switch Control Panel Chamber Gas Sample Port Water Bottle Humidity System Fold down Storage Area Humidity System Drain Leveling Feet Figure 1 1 Steri Cult Components The incubator s major components are described below Chamber Gas Sample Port Used for sampling chamber CO2 content using a FYRITE or similar instrument Power On Off Switch Mains disconnect Control Panel Keypad Displays amp Indicators Figure 1 3 Fold down Storage Area Store manuals markers
15. Press Enter to save the RS485 address Press the Mode key to return to Run mode or right left to go to next previous parameter Thermo Scientific Section 4 Configuration Chart 4 1 Configuration Mode Press MODE to light Configuration To Configure Audible Alarm NEW HEPA Filter REPLACE HEPA Timer Access Code Mode Run Settings Calibrate Configuration SYSTEM CONFIG Enter AUDIBLE ON OFF 2 Enter v Press to return to previous parameter NEW HEPA XXX e Enter Press lt to return to previous parameter REPLACE HEPA XX Enter gt v Press lt to return to previous parameter ACCESS CODE XXX Enter v Press to return to previous parameter continue on next page Thermo Scientific Audible alarm ON Press Enter to save the setting Audible alarm OFF Press Enter to clear display and reset timer Numbers increase Press Enter to save the chosen of months Numbers decrease Numbers increase Press Enter to save the setting Numbers decrease 7023307 Steri Cult 4 9
16. is displayed in the message center 3 Press the up down arrow key until the desired temperature setpoint is displayed 4 Press Enter to save the setpoint 5 Press the Mode key until the Run indicator lights for Run mode or press the right left arrow keys to go to next previous parameter Thermo Scientific 7023307 Steri Cult 2 1 Section 2 Incubator Settings Setting the Overtemp Setpoint 2 2 7023307 Steri Cult Caution The independent overtemp system is designed as a safety to protect the incubator only It is not intended to protect or limit the maximum temperature of the cell cultures or customer s equipment inside the incubator if an overtemp condition occurs The Steri Cult incubators are equipped with a secondary temperature monitoring system to monitor the air temperature inside the cabinet This system is designed as a safety device to turn off all heaters in the event of a temperature control failure Temperature control in the incubator will be 1 of the overtemp setpoint The overtemperature is set by the factory default at 40 C However the overtemp can be set up to 55 C in 0 1 increments If the incubator s operating temperature setpoint is set above the overtemp setpoint the overtemp setpoint will automatically update to 1 C above the temperature setpoint It is recommended that the overtemp setpoint be maintained at 1 C over the operating temperature setpoint To change the Overtemp se
17. Caution Before making any calibration or adjustments to the unit it is imperative that all reference instruments be properly calibrated Calibrating the Before calibration allow the cabinet temperature to stabilize Place the calibrated instrument in the center of the chamber The instrument Temperature should be in the airflow not against the shelf Temperature Stabilization Periods Startup Allow 12 hours for the temperature in the cabinet to stabilize before proceeding Presently Operating Allow at least 2 hours after the display reaches setpoint for temperature to stabilize before proceeding Thermo Scientific 7023307 Steri Cult 3 1 Section 3 Calibration 3 2 Temperature continued Calibrating the Infrared C0 System 7023307 Steri Cult Press the Mode key until Calibrate indicator lights Press the right arrow until TEMP CAL XX XC appears in the message center Press up down arrow to match display to calibrated instrument Press Enter to store calibration Press the Mode key to return to Run or the right left arrow to go to next previous parameter All models of the Steri Cult incubator have an infrared IR CO sensor Infrared sensors are not effected by chamber atmosphere temperature or humidity Sensor Stabilization Times Start Up Allow the CO2 of the cabinet to stabilize at least 12 hours before proceeding Presently Operating Allow CO to stabilize at least 2 hours at
18. Lysol No Rinse Sanitizer Spray cleaner on a clean soft cloth and wipe each sensor Caution Do not saturate the sensors or immerse the sensors in a cleaner A 13 Open the side panel and replace the sensor hole plugs into their clips Install the cleaned RH and sensors in their respective holes Be sure the sensors reach the hole limit Caution RH and CO sensors must be reinstalled correctly for systems to properly sense chamber conditions If not RH and levels may be uncontrolled 7023307 Steri Cult 8 5 Section 8 Sterilization Cycle Sterilization Cycle 14 Open the chamber and replace the present HEPA with a new one For continued instructions to install see Section 1 15 Install the new access port filter assembly into the upper left corner of the back of the chamber Section 1 Press ENTER 16 When ENTER is pressed the cycle initiation button goes out the display returns to SYSTEM OK and the HEPA filter replacement timer is reset 17 Reinstall the inventory system magnet plate if used 18 Allow to stabilize for at least 2 hours at the required temperature and CO level 19 Verify the calibration of the sensors See Section 3 or RH Note The sterilization process can cause the inner door knob to become difficult to turn A small amount of mineral oil vegetable oil or similar oil applied to the knob should remedy this 8 6 7023307 Steri Cult Thermo Scientific Sectio
19. Press Enter nter to save setting gt Numbers decrease XN Press to return to previous parameter Percent Numbers increase Relative Humidity RH XX E Dress Enter nter to save setting Numbers decrease TN Press to return to previous parameter 2 4 7023307 Steri Cult Thermo Scientific Section 3 Calibration After the unit has stabilized several different systems can be calibrated In Calibration mode the air temperature CO2 and RH levels can be calibrated to reference instruments To access Calibration mode press the Mode key until the Calibrate indicator lights Press the right and or left arrow until the appropriate parameter appears in the message center See Chart 3 1 at the end of this section for more detail Calibration frequency is dependent on use ambient conditions and accuracy required A good laboratory practice would require at least an annual calibration check On new installations all parameters should be checked after the stabilization period Prior to calibration the user should be aware of the following system functions While the unit is in Calibration mode all system control functions are disabled so the unit remains stable Readout of the system being calibrated will appear on the message center If no keys are pressed for approximately five minutes while in Calibration mode the system will reset to Run mode so control functions are reactivated
20. REPLACE HEPA filter replacement timer can be set for a specific amount of time i H from 1 to 12 months of actual unit running time Time will not accrue HEPA Filter Reminder when the unit is turned off The default time is 6 months When the allotted time has run out REPLACE HEPA appears in the display and the visual alarm flashes To set the reminder use the following procedure 1 Press the Mode key until the Configuration indicator lights 2 Press the right arrow until REPLACE HEPA XX is displayed 3 Press the up down arrow to choose the number of months desired 4 Press Enter to save the number 5 Press the Mode key to return to Run Mode or right left to go to next previous parameter Note After the reminder has been set check the allotted time remaining by going to Configuration mode then pressing the right arrow until NEW XXX displays This number is the remaining days before the filter replacement time specified runs out For example if 12 months was chosen in the REPLACE HEPA XX message screen the NEW number would be 365 days A Setting an Access 3 digit Access Code can be entered to avoid unauthorized personnel Code from changing the setpoints calibration or configuration A setting of 000 disables the access code function The factory setting is 000 1 Press the Mode key until the Configuration indicator lights 2 Press the right arrow until ACCESS CODE XXX is displayed in the message cent
21. RH Relay OFF Press to return to previous parameter Tank Select Press to return to previous parameter jH Tank 1 prime Press Enter to save the setting Tank 2 prime Gas Guard ON Press Enter to save the setting Gas Guard OFF Press lt to return to previous parameter Press to return to previous parameter Numbers increase Press Enter to save the RS485 address Numbers decrease 7023307 Steri Cult 4 11 Thermo Scientific Section5 Alarms The Steri Cult incubator alarm system is shown in the table below When an alarm is active the message appears in the LED message center Pressing Silence disables the audible alarm for the ringback period However the visual alarm continues until the incubator returns to a normal condition The alarms are momentary alarms only When an alarm condition occurs and then returns to normal the incubator automatically clears the alarm condition and the message center Table 5 1 Alarms and Descriptions Description Message Delay Ringback Relay No alarm condition exists or RH WAITS ON TEMP Temp gt OVERTEMP Setpoint SYSTEM IN OTEMP 0 min 5 min Yes Temperature Controller Failure TEMP CNTRL ERROR 0 min 5 min Yes Air Temp Sensor Fault AIR SENSOR ERROR 0 min 5 min No C02 Sensor Fault 02 SENSOR ERROR 0 min 5 min No RH Conditioner Sensor Fault RH COND SNSR ERR 0 min 5 min No Water Bottle Empty WATER EMPTY 5 min 5 min
22. Scientific Section 4 Configuration Several features available in the Configuration Mode allow custom setup of the incubator These features are listed and described below All features may not be necessary in all applications but are available if needed To enter Configuration mode press the Mode key until the Configuration indicator lights Press the right and or left arrow until the appropriate parameter appears in the message center See Chart 4 1 for more detail The audible alarm can be turned on or off The factory setting is ON 1 Press the Mode key until the Configuration indicator lights 2 Press the right arrow until AUDIBLE ON OFF is displayed in the message center 3 Press up down arrow to toggle ON or OFF 4 Press Enter to save the setting 5 Press the Mode key to return to Run mode or right left to go to next previous parameter When the REPLACE HEPA reminder is displayed and the visual alarm flashes the specified time has elapsed and the HEPA filter should be replaced After replacing the HEPA filter with a new one clear the display and reset the timer by following the steps below 1 Press the Mode key until the Configuration indicator lights 2 Press the right arrow until NEW HEPA XXX is displayed in the message center 3 Press Enter to restart the timer and clear the REPLACE HEPA alarm 4 Press the Mode key to return to Run mode 7023307 Steri Cult 4 1 Section 4 Configuration Setting the
23. Section 4 Configuration Configuration Mode Chart 4 1 Page 2 of 3 refer to previous page To Configure Numbers increase peni TeMPLoLmT xx LO LMT X X Low Temp Limit Alarm sy Press Enter to save the setting Numbers decrease Press to return to previous parameter TEMP RELAY ON OFF Temp relay ON Press Enter to save the setting Temp relay OFF Temp Alarm Relay Press to return to previous parameter CO Low ini Alarm Press Enter to save the setting Numbers decrease Numbers increase Press lt to return to previous parameter C0 High Limit lam Press Enter to gt save the setting Numbers decrease Numbers increase Press to return to previous parameter gt C02 alarm relay ON CO Alarm Relay CO2 RELAY ON OFF Press Enter to save the setting C02 alarm relay OFF Press to return to previous parameter refer to next page 4 10 17023307 Steri Cult Thermo Scientific Thermo Scientific Section 4 Configuration Configuration Mode Chart 4 1 Page 3 of 3 refer to previous page To Configure Numbers increase save the setting RH Low Limit Alarm Numbers decrease Press to return to previous parameter Numbers increase RH HI LMT X X Press Enter to save the setting Numbers decrease RH High Limit Alarm Press to return to previous parameter RH Relay ON RH RELAY ON OFF RH Alarm Relay ress Enter to save the setting
24. Thermo Scientific Filling the Humidity Water Bottle continued Connecting the 0 Gas Thermo Scientific Supply Section 1 Installation and Start Up Distillation systems as well as some types of reverse osmosis water purity systems can produce water in the quality range specified Tap water is not recommended as it may contain chlorine which can deteriorate the stainless steel Tap water may also have a high mineral content which would produce a build up of scale in the pan High purity or ultra pure water is not recommended as it is an extremely aggressive solvent and will deteriorate the stainless steel High purity water has a resistance of above 1M to 18M Ohm Even high purity water can contain bacteria and organic contaminants Water should always be sterilized or treated with a decontaminant safe for use with stainless steel as well as safe for the product prior to being introduced into the humidity bottle Use of chlorinated water or decontamination products containing chlorine will deteriorate the stainless steel and cause rust voiding the warranty Warning High concentrations of CO gas can cause asphyxiation OSHA Standards specify that employee exposure to carbon dioxide in any eight hour shift of a 40 hour work week shall not exceed the eight hour time weighted average of 5000 PPM 0 5 CO The short term exposure limit for 15 minutes or less is 30 000 ppm 396 CO Carbon dioxide monitors are recommended
25. Water Bottle 6 Turn power off 7 Attached to the bottom of the water bottle is a length of tubing and low voltage wiring Figure 7 2 Disconnect the tubing by pressing inward on the collar where the tubing connects to the bottle Pull the tubing free Disengage the wiring at the connector 8 Lift the water bottle from the bottle platform Empty any water out of the bottle 9 Replace the filter in the lid by first 280 unscrewing the clear plastic nut on the Q Plastic nut outside of the lid Catch the filter as it loosens from the inside of the lid Remove the small black o ring from the filter and O ring discard the filter Assemble this o ring onto the new filter and install as previously Air filter Tighten the plastic nut only finger tight to Figure 7 3 Filter Assembly prevent stripping 9 Clean the humidity bottle and lid with soap and water and a general use laboratory disinfectant Rinse with sterile water and spray with 70 alcohol Thermo Scientific 7023307 Steri Cult 7 3 Section 7 Routine Maintenance Cleaning the Humidity 10 Also behind the access door is the humidifier System continued See Figure 7 4 Lift the latches on both sides of this small unit 11 Separate the top and bottom sections Carefully clean the inside of both sections and spray with 70 alcohol Latch Caution Do not touch the metal disk in the Figure 7 4 Humidifier bottom of the humidifier with bare fingers as any f
26. dO NI ANY 5 SI ALNVHSIVM SIHL uoneunsep god peddius sued pue pied eBejsod ouueu peuunjeJ eq sued Buruuojuoo uou uondo s ouueu 3ueuudinbe Aue AIB 1snui 29 eu poled y puo eq qjueuoduJoo y o JO jueuudinbe y Aju amp JueM y jou jjeys AjUeJJeM siy Jepun syed sjueuoduioo Jo AJUBIIEM siy pepnjoxe sjexseb pue ssej sway ejqepuedx3 Aue Jo pue uoneuiuuejep eq s olAJ18S eoiuuoe eu siu JOU uoneJqijeo pue uonejejsu sued sie 10 aq Jewo q 94 juo sued Weak puooes e JO IM JOSUBS 00 eu euo Jo poled uenem jenu Joye uj Buipnjoui esuedxe sjouueu p je peoejdajJ Jo diysuewyJom Jo sjeuejeui ui Buruuojuoo uou eq o ue oJd sued jueuoduioo 5 Buunq poued seek y Buunp juenbesqns Aue o uonoejoud uenem
27. for confined areas where concentrations of carbon dioxide gas can accumulate A Warning This incubator is designed to be operated with CO gas only Connecting a flammable or toxic gas can result in a hazardous condition Gases other than CO should not be connected to this equipment CO2 gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve Check the gas cylinder for the proper identification labels The CO gas supply being connected to the incubator should be industrial grade 99 596 pure nominal Do not use CO gas cylinders equipped with siphon tubes A siphon tube is used to extract liquid CO from the cylinder which can damage the pressure regulator Consult with your gas supplier to ensure that the CO cylinder does not contain a siphon tube Gas cylinders should also be secured to a wall or other stationary object to prevent them from tipping two stage CO pressure regulator is required to be installed on the outlet valve of the gas cylinder Input pressure to the incubator must be maintained at 15 psig 103 4 kPa 5 psig for proper performance of the CO control system single stage CO pressure regulator will not maintain 15 psig 103 4 kPa to the incubator as the pressure in the CO cylinder decreases therefore a two stage regulator is recommended A 7023307 Steri Cult 1 11 Section 1 Installation and Start Up Connecting the 0 Gas Supply continued Humidity an
28. for tissue cultures Refer to Manual Action Daily Weekly Monthly 6 Yearly Section Months Check tank levels Perform preventive maintenance per instruction sheet 7270102 v using PM kit 22270102 2 Verify and document humidity and temperature v calibration as applicable 6 Disinfect the interior of the incubator Replace AquaTec water cleaning cell if applicable 7 Perform Sterilization Cycle as needed 6 2 7023307 Steri Cult Thermo Scientific Cleaning the Incubator Interior Thermo Scientific Section 7 Routine Maintenance Warning If the unit has been in service turn it off and disconnect the power cord connector before proceeding with any maintenance A Caution Before using any cleaning method except those recommended by the manufacturer users must check with the manufacturer that the proposed method will not damage the equipment A Use an appropriate disinfectant All articles and surfaces must be thoroughly cleaned rinsed with sterile water and rough dried Warning Alcohol even a 7096 solution is volatile and flammable Use it only in a well ventilated area that is free from open flame If any component is cleaned with alcohol do not expose the component to open flame or other possible hazard A Warning Do not use strong alkaline or caustic agents Stainless steel is corrosion resistant not corrosion proof Do not use solutions of
29. presses the Silence key However the Tank Low indicator on the control panel stays lighted until the condition is resolved The unit operates normally Caution Both the audible and visual alarms described above do not ring back after the Silence Silence key is pressed Figure 9 7 Tank Low Indicator If the Gas Guard system does not detect an adequate gas supply at CO2 Inlet 1 or Inlet 2 a visual and audible alarm again occurs on the control panel The visual alarm in the Message Center reads Tank 1 amp 2 Low The audible alarm continues to ring until the Silence key is pressed The audible alarm rings back every 15 minutes after the alarm is silenced if the Gas Guard continues to detect that both gas supply pressures are below 10 psig 0 690 bars 1 To install the datalogger sensor P N 201912 locate the sensor and cable in the bottom of the incubator chamber 2 Insert the grommet provided into the only round hole in the side of the diffuser pan m Then route the sensor through this grommet from the bottom of the diffuser pan 4 Place the datalogger sensor in the desired location or use the clip provided See Figure 9 8 clip shown on pilaster 5 See the ELPRO operating manual for settings LI LI 4 Figure 9 8 Sensor Thermo Scientific Thermo Scientific Section 10 Specifications Temperature Conole stake ou uscite ev AM ua 0 1 37 0 C Setpoint Digital touch p
30. setpoint before proceeding 1 Measure the CO concentration in the chamber through the gas sample port with a Fyrite or other independent instrument Several readings should be taken to ensure accuracy Press the Mode key until the Calibrate indicator lights Press the right arrow until CO2 CAL XX X appears in the message center Press the up down arrow to adjust the display to match the independent instrument reading Press Enter to store the calibration Press the Mode key to return to Run mode Thermo Scientific Calibrating Relative Thermo Scientific Humidity RH Section 3 Calibration Place an accurate independent instrument in the center of the chamber Most RH measuring devices are least accurate above 90 It is recommended that when calibrating above 90 the incubator should be adjusted to the lower edge of the instrument accuracy range For example with the incubator set at 95 the independent instrument with accuracy of 5 reads 94 Calibrate the incubator display to 99 This prevents condensation issues caused by actual RH levels above 95 Relative Humidity Stabilization Times Start Up Allow 12 hours for the relative humidity and temperature in the chamber to stabilize before proceeding Presently Operating Allow at least 2 hours after temperature display reaches setpoint for relative humidity to stabilize before proceeding 1 Press the Mode key until the Calibrate indicator li
31. sodium hypochlorite bleach as they may also cause pitting and rusting A filter replacement kit see parts list section should be on hand prior to cleaning the interior of the incubator 1 Remove the shelves access port filter assembly filter and the left and right pilasters Discard the HEPA filter and the access port filter assembly 2 Wash the shelves and pilasters with disinfectant then rinse with sterile water Optional The shelves and pilasters may be autoclaved 3 Wash the inner door gasket with disinfectant This gasket may be removed to be cleaned or replaced 7023307 Steri Cult 7 1 5 ction 7 utine Maintenance Cleaning the Interior continued Cleaning the Cabinet Exterior Cleaning the Humidity 7 2 7023307 Steri Cult System 4 Wash the cabinet interior with disinfectant starting at the top and working down Refer to the disinfectant label for instruction on the length of time needed before rinsing Wash the inner door s both inside and out The cabinet and door must be rinsed with sterile water until the disinfectant has been removed After the cabinet has been rinsed spray with 70 alcohol Some precautions in the cleaning and care of the incubator glass doors Moisture leaches alkaline materials sodium Na from the surface of the glass Evaporation of the moisture concentrates the alkaline and may produce a white staining or clouding of the glass surfac
32. 0 NI 1335 9 39N1H 300 NN UiHSVM UJHSYM 3AVM YIHLONY 0300 ING If 2 11 11 8S0 NI 2 z 31111 9NIMVUO 3 1 38 ANG NW 120 61 01 2506 NI 0114142530 LUV ON l Vd an 011210084 401 3573738 ANG NW NN 2 7 6 VIN 0 NOISIA38 10 0114142530 044 0 2 18 31 0 ON N23 3U 51 10 11 8 8 1 61 0011066 U38WnN ON 7023307 Steri Cult 11 6 Section 11 Servicing d E OSLSP OIHO V113IUVM 699 XOG 8 1 91 0010158 TOY 2725 ESIINNY 3715 MIGNON 9NIMVUG 031112145 3SIW83H10 552180 3 NYU3101 2154110312 5 VIN JeusiJouueu L A18N3SSV HON38 TVIUJIVW 05270 31V2S 2 S 6 11 0 092 WA NAQ SOWRASIY MOYE 80 68 BHIN NAL IRIN 1008118 1504404 ONIYNLIVINNVN 801 0150 4000 SSV19 YANNI 170218315 398V 31111 OMG YON 25048104 ANY 801 543810 OL 015012510 38 E 01 LON S NOIIVNUOINI HONS ONY NOILVMUOJNI Suo1vgnoN 110218315 1188 0188 3NVN 1uvd 1300N AYVLIIYdOYd SNIVINOO 1 380204 SIHL SEOCI Nid 161 ALY ISN LNW 310H M 31 14 BONY 3000 55719 YINN 0061 21 9t 83 5 193 3 09111 00 2 1 980081 ll 6 00 1077 X 01 882 YJHSYM 3AVM 160081 01 1 11 SS 01 682 9500 lt 6 9819201 2
33. 010314 26 610019 8 3925 41111 4 GH 55041 8 1 1 X 26 8 550056 335 41111 4 GH 550141 P E X 9 750056 9 0 0 11 832 45 133 220621 5 1V130 4431 H9IH Vul 210021 H913H 39NIH 69 JONIH SS 601911 10051 13 0838 522 201 734101 10 10 1868 E A 3 EE 8000 10 9 03N91 303U ANG 2 61 21 890 NI 2 ON WJHSVM YIHSYM JAVA YIHLONY Q30Qv INO IP 2 11 11 950 NI NOI1dIu2S30 139 ON 184 11210034 803 15 3138 ANG WW 27776 WIN 0 0151 38 40 0114182530 044 0 2 48 310 ON 23 38 SV KERS 10 11 19 8 1 61 00101 u38NfIN OMG 11 7 7023307 Steri Cult ientific Thermo Sc Section 11 Servicing OPTIONAL RS 485 CONNECTION RH CONDITIONER PROBE CONNECTION 430337 RIBBON CABLE 270106 DISPLAY FERRITE OPTIONAL GAS GUARD 1900153 USE NYLON SPACERS THIS END ONLY W 400119 1900153 m HARNESS WIRING 3308 3311 WIRING 3308 3311 TRANSFORMER WIRING DETAIL ELECTRICAL BACK PANEL ASSEMBLY 3307 3310 P HARNESS 1900146 AN OPTIONAL GAS GUARD CONNECT ION 9 0 65 485 1900152 USE METAL SPACERS THIS END ONLY W 400119 Se ES 3307 3310 ANCHOR WIRES HARNESS 3308 3311 SEE DETAIL DISCONNECT FROM BROWN
34. 1 Installation and Start Up Stacking the Incubators 1 6 7023307 Steri Cult Warning With incubators in a stacked configuration do not leave both exterior doors open at the same time A Warning If the units have been in operation turn them both off and disconnect the power before beginning any service work Four stacking brackets one shown at right are included 0 Q in the parts bag shipped with each incubator 0 1 Unscrew and remove the 4 leveling feet from the unit 0 to be stacked on top and lift it onto the base unit Figure 1 4 Align all sides Stacking Bracket Warning This incubator weighs 340 lbs for Models 3307 amp 3308 410 lbs for Models 3310 amp 3311 Have sufficient personnel available when lifting Lift the unit by the sides of the cabinet base to avoid placing stress on the outer door hinge 2 Remove the hole plugs from the stacking bracket holes Figure 1 6 3 Align the holes in the brackets with the mounting holes on the sides of the top and bottom incubator Secure the two brackets with To the stainless steel screws and washers provided in R Unig the parts bag See Figure 1 5 QW i It 4 The stacked incubators are ready for service Figure 1 5 Stacking Brackets Installed 2 Door lt E ica qu Figure 1 6 Sides and Back of Stacked Units Thermo Scientif
35. 49 RH SENSOR CONNECTION TEMP PROBE CONNECTION SENSOR DETECT CONNECTION 3307 71 2 0 REY 18 Poge 2 of 2 7023307 Steri Cult Thermo Scientific Section 11 Servicing R 400119 400132 FOR KIT 190779 Hi M POWER CONNECTION 115V C 50 60HZ 10 5 FLA 3307 11 5 3310 INLET RFI LINE FILTER 480180 POWER SWITCH CIRCUIT BREAKER 15 230196 EXTERIOR CONVENIENCE OUTLET 460024 INTERIOR FAN MOTOR 115 50 60Hz 156125 COMPONENT COOLING FAN 130625 CHAMBER HEATERS CONTROL HEATER ELEMENT CHART DyERTEMP SAFETY MANUAL RESET on Rise 400150 FRAME HEATER RH CONDITIONER HEATER DOOR HEATER DOOR HEATER STERI CYC CHAMBER HEATERS STERI CYC HEATER ELEMENT CHART HEATER STERI CYC TRANSFORMER 56 420108 LLTRA SONIC HUMIDIFIER ASSEMBLY ULTRASONIC SAFEGUARD FLOAT SWITCH PART ASSEMBLY H20 FILL SOLENOID 12700 250049 CO2 SOLENOID 2700 250139 ADD WATER ELLE REMINDER 280095 ULTRASONIC FAN PART HUMIDIFIER ASSEMBLY 900140 HUMIDITY VALVE 250128 Electrical Schematic Model 3307 amp 3310 Steri Cult II Incubator 3307 70 0 D Rev 11 Page 1 of 3 Thermo Scientific 7023307 Steri Cult 11 10 Section 11 Servicing 39 ie 40 41 rhe reel a 42 A 43 ATER LEVEL SWITCH 44 45 E c 46 47 48 TEMP PROBE 2252 DM e25
36. 7 Steri Cult 8 3 Section 8 Sterilization Cycle Sterilization Cycle continued Figure 8 3 Heat Phase in Sterilization Cycle 9 When the air temperature in the unit reaches 140 0 C the Sterilization Phase begins and the display shows STERILIZING 140 10 After approximately 2 hours 5 second audible tone sounds signaling that sterilization is complete The Cool Phase begins The display toggles between STERILIZING and COOL PHASE STERILIZING Figure 8 4 Cool Phase in Sterilization Cycle 8 4 7023307 Steri Cult Thermo Scientific Sterilization Cycle continued Thermo Scientific Section 8 Sterilization Cycle 11 Completed Sterilization Cycle When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYCLE COMPLETE REPLACE HEPAs INSTALL SENSORS and PRESS ENTER The cycle initiation button is lighted but no longer flashes CYCLE COMPLETE a Enter REPLACE HEPAs Enter INSTALL SENSORS a Enter PRESS ENTER Enter Figure 8 5 Cycle Complete 12 Disinfect the RH and sensors with either isopropanol or
37. AND ANCHOR IN HARNESS y HARNESS 1900145 lt lt 7 TO BLK TEFLON LEADS OF T STAT CONNECTION TD BACK HEATER ORG BLK GRY WHT r CONNECTION RIGHT HEATER ORG BLK RED RED GRY WHT CONNECTION TO TOP HEATER MODELS 3310 3311 URG BLK REO REO GRY WHT UN MODELS 3307 3308 INSTALL 210088 INSTALLED HROUGH EXTERIOR OF OUTER WRAP 230201 75A FUSE 220313 75A LABEL FUSE HOLDER DETAIL 2 3307 3310 230135 1A FUSE 220359 1A LABEL 3308 3311 230120 0 54 FUSE 220378 0 54 LABEL CONNECTION LEFT HEATER URG BLK RED RED GRY WHT CONNECTION 10 BOTTOM HEATER ORG BLK RED RED GRY WHT URG BLK RED RED GRY WHT TD DOOR CABLE 3307 71 1 D REV 18 Page of 2 CONNECTION PLENUM HEATER Thermo Scientific 7023307 Steri Cult 11 8 Section 11 Servicing 11 9 HARDWARE CHAR SYM STK DESCRIPTION SYM STK DESCRIPTION A 22050 6 32 1 2 PAN HD PHP SCREW u 515084 SPACER 1 400 X 3 8L B 59007 4 40 X 3 8 PAN HD PHP SCREW R 590032 4 40 1 4 PHP SEM SCREW C 23002 88 32 NUT W EXT LK WASHER S 350006 10 32 X 1 8 BRASS HOSE FITTING D 23006 4 40 KEPS W EXT LK WASHER dl 23024 3 8 SS FLAT WASHER E 22049 6 32 X 3 8 PAN HD PHPS SCREW u 210089 22 18 FULLY INS 250 TAB 127039 8 32 3 16 5 5 SPACER 188060 TUBE ASS Y
38. C CONTROL 290185 AIR TEMP 49 RH CONDITIONER PROBE 2252 DHM 25C 290185 50 51 52 s 2222 53 IR amp RH SENSOR DOR DETECT 54 360283 2 SENSORS IN PLACE 55 RH SENSOR 290174 56 57 58 REMOTE ALARM CONNECTIONS 59 shown in olorn stote 4b 61 lt RII OPTIONAL 85 485 OUTPUT KIT A 1900152 E 3 CRS 485 62 63 ET 64 58 OPTIONAL GAS GUARD KIT 1900153 02 65 53 NL ono PRESSLRE SWITCH 1 EMPTY 66 50 PRESSURE SWITCH 42 TANK EMPTY EX 360213 67 62 SOLENOID 3 yAY 12yDC 68 250121 69 70 71 72 Electrical Schematic Model 73 OPTIONAL ANALOG KITS 3307 amp 3310 74 ae aeons Steri Cult II Incubator 91763 0 to 75 3307 70 0 Rev 11 76 Page 2 of 3 11 11 7023307 Steri Cult Thermo Scientific Section 11 Servicing 77 WIRE REFERENCE CHART GA cum GA cum Nr cA co 78 1 16 BROWN 4 20 PURPLE 49 22 GREEN 6 25 20 9 22 79 3 16 26 20 vero 51 22 GREEN 44 18 BACK 2 20 52 22 BLACK 80 4B 20 BLACK 2 20 RED 53 2473 BLACK 5A
39. Check to ensure tubing is not pinched or dis connected Clean the humidifier see Section 6 Ensure RH sensor bottoms out in hole Ensure there are not gaps between the gasket and the glass door Calibrate the RH see Section 3 Ensure RH sensor bottoms out in hole 7023307 Steri Cult 6 1 PREVENTIVE MAINTENANCE Incubators Your equipment has been thoroughly tested before shipment Regular preventive maintenance is important to keep your unit functioning properly The operator should perform routine cleaning and maintenance on a regular basis For maximum performance and efficiency it is recommended the unit be checked and calibrated periodically by a qualified service technician The following is a condensed list of preventive maintenance requirements See the specified section of the instruction manual for further details We have qualified service technicians using NIST traceable instruments available in many areas For more information on Preventive Maintenance or Extended Warranties please contact us at the number listed below Cleaning and calibration adjustment intervals are dependent upon use environmental conditions and accuracy required Tips for all incubators Do NOT use bleach or any disinfectant that has high chloros e Use sterile distilled or demineralized water Preventive Maintenance for Steri Cult Incubators Avoid spraying cleaner on the sensor Do not use powdered gloves
40. IFIED DRAWING NUMBER SIZE SENSITIVE DEVICES BDX 649 MARIETT DHID 45750 ANGLES 3308 70 0 0 Electrical Schematic Model 3308 amp 3311 Steri Cult II Incubator 3308 70 0 D Rev 11 Page 3 of 3 Thermo Scientific 7023307 Steri Cult 11 15 60 v E SERES DERI L006 081 JO e90 INOA WSN eui episino SUOR eoidde eioeds Ajueuew jueuudinbe uo suonsenb noA JeMsue 9 0 10 epeue2 pue YSA 008 1e sanag INOA eseejd p nb s jueuudinbe uonejejsur jueuudinbe paud uoneuuojui 4 djeu o si 20 INOA SjonpouJd 10 550 Jo syyoud 1so o uonejiui jenuenbesuoo Aue JO ejqei 100 oynuerog 19514 AdddV TIVHS 5 YVINDILYVd SSANLIA ALEFTISBVLNVHO HIN dO SAILNVYYVM ON N3 LLIHM YSHLSHM S3ILNVHSVM 1
41. ION 230VAC 50 60HZ 5 4 FLA 3308 5 9 FLA 3311 POWER INLET RFI LINE FILTER 460180 POWER SWITCH CIRCUIT BREAKER 10 230179 EXTERIOR CONVENIENCE OUTLET 460138 320 5 INTERIOR FAN MOTOR 115 50 6092 156125 COMPONENT COOLING FAN 130625 ALITO TRANSFORMER 43VA 420097 CHAMBER HEATERS CONTROL SEE HEATER ELEMENT CHART DVERTEMP SAFETY MANUAL RESET Sa on Rise 400150 FRAME HEATER RH CONDITIONER HEATER DOOR HEATER DOOR HEATER STERI CYC CHAMBER HEATERS STERI CYC SEE HEATER ELEMENT CHART PLENUM HEATER STERI CYC TRANSFORMER 30V 56 420108 ULTRA SONIC HUMIDIFIER ASSEMBLY ULTRASONIC SAFEGUARD FLOAT SWITCH PART OF ASSEMBLY COR H20 FILL SOLENOID 12VDC 250049 2 SOLENOID 12VDC 250139 ADD WATER BLUE REMINDER 280096 ULTRASONIC HUM FAN PART ASSEMBLY 900140 HUMIDITY VALVE 250128 Electrical Schematic Model 3308 amp 3311 Steri Cult Il Incubator 3308 70 0 D Rev 11 Page 1 of 3 Thermo Scientific 7023307 Steri Cult 11 13 Section 11 Servicing 11 14 B O
42. O 3AISN19X3 SI ALNVHSIVM SIHL uoneunsep god peddius sued pue pied eBejsod ouueu peuunjeJ eq sued Buruuojuoo uou uondo s ouueu 3ueuudinbe Aue AIB 1snui jueunjedeq seoriues eoiuuoe eu poled y puo eq qjueuoduJoo y o JO jueuudinbe y y jou 5 AUeJJe siy Jepun 3ueuudinbe sued sjueuoduioo Jo edas 10 siy pepnjoxe sjexseb pue sey ssej6 sway ejqepuedx3 Aue jo eoueuuopged oj uon pue eq samas eoiuu29 94 siy Aq jou SI uoneJqijeo pue uonejejsu sued sjee 3931y 10 aq Jewo q 94 juo Sed puooes JO JOSUBS 200 eu euo poued Joye uj oqe Bundeoxe esuedxe s ouueu je Jo eq Jo ui Buruuojuoo uou eq sued 51 eu Buunq ponad seek ysi Buunp aumo juenbesqns Aue o uonoejoud uenem
43. O alarm limit 5 Press the Mode key to return to run mode or right left to go to next previous parameter Thermo Scientific Section 4 Configuration Enabling CO Alarms High and Low CO alarms be programmed to trip the remote alarm i contacts A setting of ON activates this ability setting of OFF will not to Trip Contacts allow CO alarms to trip the contacts The factory setting is ON 1 2 Press the Mode key until the Configuration indicator lights Press the right arrow until CO2 RELAY ON OFF is displayed in the message center Press up down arrow to toggle the setting ON or OFF Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter Setting Low RH The low RH alarm limit tracking alarm is the deviation from the rm Limit humidity setpoint which causes a low RH alarm The setpoint is variable Ala from 5 to 20 RH The factory setting is 10 RH 1 2 Thermo Scientific Press the Mode key until the Configuration indicator lights Press the right arrow until RH LO LMT XX is displayed in the message center Press up down arrow to change the RH low alarm limit Press Enter to save the RH low alarm limit Press the Mode key to return to Run mode or right left to go to next previous parameter 7023307 Steri Cult 4 5 Section 4 Configuration Setting a High RH The high RH alarm limit tracking alarm is the deviation f
44. OHMS mas Z LEFT 5 132086 8 0 6281 88 6943 13 47 8 1051 36 1162 03 103 RIGHT SIDE 132087 12 4 4052 62 4479 44 70 8 709 82 784 53 BACK 32090 34 8 1444 11 1596 12 1627 308 88 341 40 132085 8 4 5982 74 6612 50 124 3 402 68 445 07 132088 13 4 3750 37 4145 15 142 2 353 41 390 61 FRAME 32084 64 3 781 57 863 64 64 3 781 57 863 84 DOOR 32089 742 57729 74858 441 7 113 78 125 75 RH COND 13211 27 6 1820 83 2012 50 WA PLENUM 32092 14 8 3395 6 3753 04 100 8 498 07 550 50 11 IN 4660 07 15 15 JAS SAG CCS 24AWG CABLES WERE 2246 10 IN 4592 10 15 14 DAG SAG LON 360283 WAS 360230 9 IN 4469 03 18 14 GLM SAG 250139 WAS 250085 B st to0076 10 08 08 GJG GJG LUN REVISED FAN STOCK NUMBER 7 18 3242 03 07 05 LUN CORRECT RH SENSOR 290154 10 290174 5 IN 3150 12 17 03 LON CHANGED INNER DOOR SWITCH FROM 360251 REV DATE BY DESCRIPTION OF REVISION d i ME In MODEL PART 3308 amp 3311 STERI CULT II INCUBATOR ATTENT I ON DIU E DS AY PROS MR DyG TITLE ELECTRICAL 5 WRITTEN PERMISSION FROM THERMO FISHER SCIENTIFIC DYN Gy cap Gy DNF 7 17 02 scie NONE OBSERVE PRECAUTIONS MATERIAL ELECTROSTATIC ThermoFisher SCIENTIFIC TOLERANCE UNLESS OTHERWISE SPEC
45. Orientation for 3307 3308 Figure 7 7b HEPA Orientation for 3310 3311 1 Locate and loosen the two large wingnuts in the top of the inner chamber Slide the filter bracket toward the chamber opening until the filter swings down 2 Lift the back edge of the HEPA filter until it unhooks from the metal lip in the back of the chamber Discard the filter 3 Install a new HEPA by first hooking the back edge of the filter as previously 4 After the back edge is hooked lift the front of the HEPA to the top of the chamber Slide the filter bracket over the HEPA frame to secure it Tighten the two wingnuts 7 6 7023307 Steri Cult Thermo Scientific Section 8 Sterilization Cycle Sterilization Cycle Information You Need to Know Before Starting a Cycle If the Sterilization Cycle is to be performed on initial start up with new unused filters do not install the access port or HEPA filters until AFTER the Sterilization Cycle If the unit has been in service the HEPA must be removed BEFORE the Sterilization cycle The filtering material cannot withstand the 140 heat It will melt onto the shelves and be difficult to remove The Sterilization Cycle requires approximately 14 hours heat up 2 5 5 hours sterilize 2 hours and cool down 8 10 hours Additional time is needed to verify the calibration of temperature and CO after the cycle is complete During the Sterilization Cycle the incubator updates the temperature to the an
46. SAT I M In MODEL PART 3307 amp 3310 STERI CULT II INCUBATOR ATTENT I ON PE Dist ono Deam ANY rem DWG TITLE ELECTRICAL SCHEMATIC WRITTEN PERMISSION FROM THERMO FISHER SCIENTIFIC OWN Gy cap Gy 20 DNF 7 17 02 NONE OBSERVE PRECAUTIONS MATERIAL ELECTROSTATIC ThermoFisher TOLERANCE UNLESS OTHERWISE SPECIFIED DRAWING NUMBER SIZE SENSITIVE DEVICES BOX 649 MARIETTA DHID 45750 ANGLES 3307 70 0 D Electrical Schematic Model 3307 amp 3310 Steri Cult II Incubator 3307 70 0 D Rev 11 Page 3 of 3 Thermo Scientific 7023307 Steri Cult 11 12 Section 11 Servicing oO ON O Ul RR 60 CO Ul E S UJ UJ UJ LJ UJ UJ UJ UJ NJ NJ CO CO O OM N 2 i 3 j e LN Al sims MEA 4B 230120 3 E C 5 141 1 e T mj PLENUM HEATER T Hp 8 H MES g H Ri peas 121 7 2 H JH 72 750 FUSE 230201 14 45 21 10 d 47 lt 48VAC 16 1 5 DISPLAY BD 19168 2 p RIBBON CABLE H m e EN Z PO ER SUPPLY 77 400119 tn 400132 FOR 4 KIT 190779 05 5 14 02 55 L Beg 5 24 _ 3 N A L 20 535 6 ja J2 2 22 J2 3 4 2 23 22 4 7 28 15 5 e CD 5 6 23 E ME 45 8 4 5 9 442 N POWER CONNECT
47. SOdelfid 9 18 12 05 035 ASSV 8NS 78 SIMOIG 31111 9MO SON 5093 9 303 583 10 OL 035012510 38 01 LON SI NOILYWXOJNI HINS ONY NOILVWaOJNI TSNVd 34078 10 01 3WVN Llavd 3004 A3V13I3dU3d SNIVINOD LNSWNIO0 5 3NDJITIS M 543825 ZE 8 WIS 37338 834078 IE 10EOIEE 6 155 8 5 110825 7 10 10 0 81 155 8 5 31979 10 10 0 Z 155 8 5 33133A30 2 10 10 91 INNON 2060061 S 9800 v USHSVM 7331 220062 1 ONIX SNINIVISY 1X3 SS 210059 21 v AGNOS dHd SS B S X O v 910065 1 ABW3SSV SOLOW 521951 OIEE NOILVINSNI SOLOW 6 13 9 110955 _ 52160 8 BIM 8 0 51 611000 Z v SSHSVANIOT HLOOL 1x3 ss 8 650 9 v SS 2 8 00 S I SS Oz wA voo v n dZ 2 6 2002 9 3525 SS 9 1 X 19022 2 3825 SS 000 1 x ze 8 91022 ALO 119192530 Lavd ON 0 SVIS3IVN 30 1118 ONTINOW 0351038 5 __ 522 2 06 zsoe Nr 1 011210084 803 35V3133 820 820 20 50 V N 0 NOISIA3Y 40 1114132530 Oddy 03 48 ON 8 6 10 10 0
48. To change the RH setpoint 1 Press the Mode key until the Settings indicator lights 2 Press the right arrow until RH is displayed the message center 3 Press the up down arrows until the desired RH setpoint is displayed 4 Press Enter to save the setpoint 5 Press the Mode key until the Run indicator lights to go to Run mode or press the right left arrow keys to go to next previous parameter On power up or a temperature setpoint change RH is delayed until the temperature is within 0 5 C of the setpoint RH WAITS ON TEMP is displayed until this temperature is reached 7023307 Steri Cult 2 3 Section 2 Incubator Settings Chart 2 1 Set Mode Press MODE to light Settings Run Settings Calibrate Configuration N T S t SET POINTS gt 0 Enter A Numbers increase Operating TEMP XX XC MUR Temperature Enter to save setting Numbers decrease Press to return to previous parameter Over Numbers increase OVERTEMP XX XC E Temperature Enter a N to save setting lt gt Numbers decrease L Press to return to previous parameter Numbers increase Percent 02 CO2 XX X
49. VAC Model 3308 5 4 FLA Model 3311 5 9 FLA Circuit breaker power switch 10A 2 Pole Model 3308 3311 Power interconnect IEC 320 male Power inlet 8 2 4m cord with IEC 320 female plug and country specific plug Accessory outlet Standard 115V 220V export 75 watts max one AN OR i chamber Alarm contacts Deviation of temperature CO2 RH power failure N O and N C Dimensions Exterior 3307 3308 35 0 W x 39 4 H x 27 0 F B 88 9cm x 100 1cm x 68 6cm 3310 3311 43 0 W x 39 4 H x 27 0 F B 109 2cm x 100 1cm x 68 6cm Weight Net weight Model 3307 3308 330 lbs 149 7kg Model 3310 3311 410 15 186 0kg Shipping motor Model 3307 3308 400 Ibs 181 4kg Model 3310 3311 480 lbs 217 7kg Certifications Declarations of Conformity available on request 7023307 Steri Cult 10 3 Section 10 Specifications 10 4 7023307 Steri Cult Safety Specifications Indoor Use Only Up to 2 000 meters Temperature 5 to 40 C Humidity Maximum 80 RH for temperatures to 31 C decreasing linearly to 5096 RH at 40 Mains Supply Fluctuations Mains supply voltage fluctuations not to exceed 10 of the nominal voltage Installation Category II Pollution Degree 2 Class of Equipment I Installation Category overvoltage category defines
50. ad 0 1 C resolution Range5 C over ambient to 50 C factory default 10 0 C disabled Uniformity 0 2 C 37 C in a 23 C ambient nominal line voltage Display Digital LED 0 1 C increments be eee uc dde Thermistor Temperature Safety Setpoint Fixed 160 C 5 6 C Thermostat manual reset Action removes voltage from heaters to disable heat control Relative Humidity sc rugs sd ture bd 2 0 a easy Ambient level to 90 Controller Microprocessor Humidity system 9596 RH at 37 Setpoint Digital touch pad 1 resolution Display Digital LED 1 resolution Contre se os m era 0 1 5 0 eee ba WR Infrared Setpoint Digital touch pad 0 1 C resolution Range 0 to 20 factory default 0 disabled Inlet Pressure 15 PSIG 5 PSIG Controller Microprocessor Display Digital LED 0 1 increments Specifications are based on nominal voltages of 115V or 230V in ambients of 22 to 25 Humidity greater than or equal to 90 may lead to condensation depending on environmental conditions in the work area 7023307 Steri Cult 10 1 Section 10 Specifications Shelves Standard 5 22 Construction
51. alog output board and the 1535 however CO and RH will be fixed at setpoint A filter replacement kit see the spare parts list should be on hand prior to initiating the Sterilization Cycle Information About the Cycle Pre cleaning may be required In order to avoid odors stains on the interior baked on material etc wipe off all visible signs of spills Odor may occur during the Sterilization Cycle and is considered normal The Sterilization Cycle is not intended to sterilize other items instruments etc from the lab During the cycle the unit chamber becomes hot enough to melt samples instruments dishes etc left inside the unit The RH and CO2 sensors also need to be removed During the Sterilization Cycle discoloration of some materials may occur For example stainless steel turns a straw color after a period of exposure to high temperatures This is normal Thermo Scientific 7023307 Steri Cult 8 1 Section 8 Sterilization Cycle Table 8 1 Checkpoints What if Cycle does not initiate or terminates in mid cycle Units are stacked prompt prompt Need to cancel cycle in progress Sensor hole plugs are not installed The outer door is opened during the Heat or Sterilization phases The outer door is open longer than 20 seconds during phases listed above The outer door is open during the Cool Down phase when the temp is 60 C or greater Power interruption during the HEAT PHASE Power
52. cal Thermo Scientific distributor iv 7023307 Steri Cult Thermo Scientific Warranty Notes Information You Should Know Before Requesting Warranty Service Locate the model and serial numbers A serial tag is located on the unit itself For equipment service or maintenance or with technical or special application inquiries contact Technical Services at 1 800 438 4851 or 1 740 373 4763 USA and Canada Outside the USA contact your local distributor Repairs NOT Covered Under Warranty Calibration of control parameters Nominal calibrations are performed at the factory typically 1 C for temperature 1 for gases and 5 for humidity Our service personnel can provide precise calibrations as a billable service at your location Calibration after a warranty repair is covered under the warranty Damage resulting from use of improper quality water chemicals or cleaning agents detrimental to equipment materials Service calls for improper installation or operating instructions Corrections to any of the following are billable services 1 electrical service connection 2 tubing connections 3 gas regulators 4 gas tanks 5 unit leveling 6 room ventilation 7 adverse ambient temperature fluctuations 8 any repair external to the unit Damage resulting from accident alteration misuse abuse fire flood acts of God or improper installation Repairs to parts or systems resulti
53. continuous cycle 8 Allow the incubator to run approximately 30 minutes to fill and agitate the solution in the humidity system Figure 7 6 Door Switch 9 Reconnect the drain fitting and drain the system again 10 Disconnect the drain fitting and fill the incubator water bottle with distilled water Allow the incubator to run for 15 minutes 11 Connect the drain fitting and allow the system to drain 12 Repeat the Steps 9 and 10 until all decontaminant is rinsed from the incubator humidity system 13 Remove the tape from the door switch Fill the humidity system per the instructions in Section 1 and return the unit to service Thermo Scientific 7023307 Steri Cult 7 5 Section 7 Routine Maintenance H EP A Fi Iter Replace the filter when the REPLACE reminder is Maintenance displayed or after a Sterilization cycle Caution The HEPA filter must be removed before the Sterilization cycle The filtering material cannot withstand the 140 heat It will melt onto the shelves and be difficult to remove A The REPLACE reminder can be set to alarm after a specified time from 1 to 12 months The reminder default is the factory recommended setting of 6 months For details on setting the reminder see Section 4 Replace the HEPA filter Figures 7 7a and 7 7b using the steps below metal lip attached to ceiling x To ceiling Filter bracket Filter bracket Wingnut Figure 7 7a HEPA
54. d Loading the 1 12 Chamber 7023307 Steri Cult Warning If higher purity CO is desired inside the incubator greater than 99 5 pure nominal the pressure regulator should be constructed with a stainless steel diaphragm along with specifying the purity of the from the gas supplier Follow the manufacturer s instructions to ensure proper and safe installation of the pressure regulator on the gas cylinder Consult a facility safety officer to ensure that the equipment is installed in accordance with codes and regulations applicable in your area A The CO gas supply being connected should be industrial grade 99 5 pure nominal and should not contain siphon tubes Install a two stage pressure regulator at the cylinder outlet The high pressure gauge at the tank should have 0 2000 PSIG range The low pressure gauge at the incubator inlet should have a 0 30 PSIG range Input pressure to the incubator must be maintained at 15 PSIG 103 4 kPa 7 The incubator has serrated fittings on the E back of the cabinet to connect the gas supply Refer to Figure 1 14 The fitting is labeled CO2 Inlet 1 Tank Make sure that the connections are secured with C02 Inlet 5 C02 Inlet clamps Check all fittings for leaks 2 TR mi 1 Tank For units having the Gas Guard option refer to Section 9 Power Inlet Connector D Figure 1 14 Connection Locations Wit
55. e Cleaning chemicals with a PH above 9 and heat autoclaving accelerate the corrosion process Therefore it is very important to rinse and dry the glass doors after cleaning Autoclaving the glass doors should be avoided There is no simple method for repairing corroded glass In most cases the glass must be replaced 5 Install the left and right pilasters inner door gasket and access port filter assembly spraying each with 70 alcohol 6 Install a new HEPA filter 7 Install the shelves and spray with 70 alcohol Clean the incubator exterior with a damp sponge or soft well wrung cloth and mild detergent dissolved in water Dry with a soft cloth Make sure power is on and the RH setpoint has been changed from factory default 1 Open outer chamber door 2 Open the access door located on the front of the unit below the control panel 3 Locate the humidity system drain fitting attached to the bottom of this door See Figure 1 13 4 Install the hose barb end of this fitting to the supplied tubing and route to a drain or suitable 0 5 gallon 3 8 liter container Thermo Scientific Section 7 Routine Maintenance Cleaning the Humidity 5 Connect the other end of the fitting to the quick connect drain located System co ntinue d below the fold down storage door See Figure 7 1 Allow water to drain This may take several minutes Humidity System Drain Y Figure 7 1 RH Drain Figure 7 2 Drain
56. er 3 Press up down arrow to change the access code 4 Press Enter to save the access code 5 Press the Mode key to return to the Run mode or right left to go to next previous parameter 4 2 7023307 Steri Cult Thermo Scientific Section 4 Configuration Setting a Low Temp The low temperature alarm limit tracking alarm is the deviation from the Alarm Limit emperature setpoint which causes a low temp alarm The low temp alarm is variable from one half degree to five degrees below setpoint The factory setting is 1 below setpoint minus sign in the display indicates that the alarm setting is below the setpoint 1 2 Press the Mode key until the Configuration indicator lights Press the right arrow until TEMP LO LMT X X is displayed in the message center Press up down arrow to change the low temp alarm limit Press Enter to save the low temp alarm limit Press the Mode key to return to Run mode or right left to go to next previous parameter Enabling Temp Alarms The high and low temperature alarms can be programmed to trip the to Trip Contacts remote alarm contacts A setting of ON activates this ability A setting of P OFF will not allow the temp alarms to trip the contacts The factory setting is ON 1 2 Thermo Scientific Press the Mode key until the Configuration indicator lights Press the right arrow until TEMP RELAY ON OFF is displayed Press the up down key to toggle the setting ON OFF Press E
57. etc Leveling Feet Used to level the unit Note The incubators are stackable Information follows in this section Thermo Scientific 7023307 Steri Cult 1 1 Section 1 Installation and Start Up Inventory Management System Control Panels Keys Displays amp Indicators 1 2 7023307 Steri Cult Included in the parts bag shipped with each unit is an inventory management system This system consists of steel plate Five different colored magnets Five heat resistant shelf labels Dry Erase marker Hang the plate on the inside of the outer door using the keyholes and hardware already installed on the door Attach a colored shelf label to the front edge of each shelf The magnets may be aligned on the plate in order according to shelf label color Use the marker to record information about the product on corresponding shelves The magnets can be erased as needed and Figure 1 2 Inventory System rewritten Caution The inventory management system magnet plate will not withstand the heat of the sterilization cycle Simply unhook the whole plate from the outer door and set aside before initiating the cycle then reinstall afterward A Heat Indicator Heat Display Inject Indicator C0 Display Humidity Indicator Humidity RH Display Model Select Switch p Run Settings Calibrate Configuration Enter Button Message Center P Up and Down Arrows Left and Right Arrows Silence Button Alarm Indicat
58. ghts 2 Press the right arrow key until RH CAL XXX appears the message center 3 Press the up down arrow to match the display to the independent instrument Press Enter to store the calibration 5 Press the Mode key to return to Run mode 7023307 Steri Cult 3 3 Section 3 Calibrate Mode Press MODE to light Calibrate Calibration To Calibrate Operating Temperature C02 Percent RH 3 4 7023307 Steri Cult Chart 3 1 Settings Calibrate Configuration CALIBRATE p a Enter TEMP CAL M Press to return to previous parameter Enter N gt 2 CAL gt Enter ec Press to return to previous parameter RH CAL S Enter Nes Press lt to return to previous parameter Numbers increase Press Enter to save the setting Numbers decrease Numbers increase Press Enter to save the setting Numbers decrease Numbers increase Press Enter to save the setting Numbers decrease Thermo Scientific Turning the Audible Alarm ON OFF New HEPA Filter Thermo
59. h the chamber loaded with liquid product and the RH setpoint at less than 90 some evaporation of product will occur If the actual RH reaches 2 above setpoint the incubator microprocessor turns on dehumidification This process pulls outside air in through the nebulizer and exhausts air through the access port Humidity decreases However in the process CO2 consumption increases Ensure your tank and product are monitored Thermo Scientific Section2 Incubator Settings With the incubator properly installed and connected to power the humidity water bottle filled and the unit connected to gas supplies system setpoints can be entered The following setpoints can be entered in Settings mode Temperature Overtemp CO2 and RH To enter Settings mode press the Mode key until the Settings indicator lights Press the right and or left arrow keys until the proper parameter appears in the message center See Chart 2 1 for more detail Setting the The temperature setpoint range is 10 C to 50 C settable to within 0 1 C Temperatu re Setpoint The lowest temperature the incubator can control is 5 C above the ambient temperature in the incubator s location The incubator is shipped from the factory with a temperature setpoint of 10 C At this setting temperature control and alarms are turned off To change the temperature setpoint 1 Press the Mode key until the Settings indicator lights 2 Press the right arrow until Temp
60. han 1 C HEAT PHASE begins if the chamber temperature has not dropped below 139 C STERILIZATION PHASE starts again when the chamber temperature reaches 140 C CANCELED COOL PHASE starts if the chamber temp dropped below 139 C INSTALL SENSORS is displayed until the sensor hole plugs are removed All control is delayed for 1 minute to allow time to reinstall the sensors RH level in chamber reaches saturation and level becomes dan gerously high CANCELED COOL PHASE The display toggles between CYCLE CANCELLED and COOL PHASE When the temperature cools down to the original set operating temperature 30 C whichever is higher the display toggles between CYCLE CANCELLED REPLACE HEPAs REPLACE SENSORS and PRESS ENTER The green LED is lighted but no longer flashes After the unit is powered back on with the sensor reinstalled the display returns to SYSTEM OK 8 2 7023307 Steri Cult Thermo Scientific Sterilization Cycle Thermo Scientific Section 8 Sterilization Cycle Warning The Sterilization Cycle will heat the incubator interior surfaces to 140 C Contact with any surface inside the outer door during this cycle may result in burns 1 Remove any samples instruments dishes etc from the chamber Set the inventory system magnet plate aside if used 2 Press and hold the Sterilization Cycle initiation button on the control panel Figure 8 1 for approximately 3 seco
61. hased with the RS485 communications option P N 1900152 This option allows the incubator to be ae directly connected to a Model 1535 alarm system without the use of a communications module A 85485 te junction box is provided with each RS485 option See Figure 9 3 for wiring details Figure 9 2 shows connector the location of the RS485 connector on the back of the incubator cabinet To allow the incubator and the 1535 to communicate an address must be allocated on the 1535 Refer to Section 5 8 of the 1535 operating z p gt manual The same address number must be assigned to the incubator Refer to Section 4 of this Figure 9 2 RS485 manual Connector location to 1535 black to additional 1535 mogues modules ml lt 2 2 orange wires 1 green wire 3 amp 1 red wire 3 yellow wires gt lt 1 black wire oy f P amp 1535 Junction gt gt 57 to incubator RJ 11 connector Figure 9 3 RS485 Wiring CAUTION Contains Parts and Assemblies L aA Susceptible to Damage by Electrostatic Discharge ESD 9 2 7023307 Steri Cult Thermo Scientific Connecting the Analog Output Boards Thermo Scientific Section 9 Factory Options Warning The electronics section contains hazardous voltages Only qualified personnel should access this area A The analog out
62. he media can be damaged if it is mishandled To avoid damage to the incubator do not operate the unit without the HEPA filter in place 1 Remove the filter from the shipping box 2 Remove the plastic covering from the filter using caution not to touch the filter media 3 Loosen black wingnuts in front corners of the chamber ceiling Figure 1 11 1 12 To ceiling Filter bracket 4 Wingnut Figure 1 11 HEPA Orientation for Model 3307 3308 metal lip attached to ceiling Filter bracket Wingnut Figure 1 12 HEPA Orientation for Model 3310 3311 4 Locate the metal lip at the upper back of the chamber Hook the HEPA filter carefully over the back edge of the metal lip 5 Lift the front of the filter to the ceiling of the chamber Push filter bracket back over the filter frame Tighten the wingnuts 6 Refer to Section 6 for HEPA filter maintenance 7023307 Steri Cult 1 9 Section 1 Installation and Start Up Filling the Humidity Water 1 10 Leveling the Unit Connecting the Unit to Electrical Power 7023307 Steri Cult Bottle Check for level by placing a bubble style level on one of the shelves Turn the hex nut on the leveling feet counterclockwise to lengthen the leg or clockwise to shorten it Level the unit front to back and left to right See the serial tag on the side of the unit for electrical specifications or refer to the electrical schematics in this
63. his manual may result in damage to the unit injury to operating personnel and poor equipment performance Caution All internal adjustments and maintenance must be performed by qualified service personnel Caution If the incubator is not used in the manner specified in this operating manual the protection provided by the equipment design may be impaired Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual 2007 Thermo Fisher Scientific All rights reserved ii 7023307 Steri Cult Thermo Scientific Important operating and or maintenance instructions Read the accompanying text carefully Potential electrical hazards Only qualified persons should perform procedures associated with this symbol Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the
64. ic Section 1 Installation and Start Up Preliminary Cleaning Using a suitable laboratory disinfectant thoroughly clean all interior surfaces Caution Before using any cleaning or decontamination method except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Accidental spills of hazardous materials on or inside this unit are the responsibility of the user Installing the Shelves 1 Install the pilasters two on each side with the tab Side facing into the center of the toward chamber with their slots up Press downward on the top section of each pilaster to locate then release when in position Figure 1 9 Fit each pilaster securely into the indentations at each side of the ceiling and the floor of the chamber Figure 1 7 shows the diffuser pan pilasters and shelf channels Side toward pilaster Shelf Channels Diffuser Pan Pilaster Pilaster NS 2 N d 202222222222222 273 2 Install the diffuser pan in the bottom of the unit with Figure 1 7 Shelf Installation the flanges down See Section 9 at this point if your unit includes a factory installed datalogger P N 201912 3 Install the shelf channels by placing the channel s rear slot over the appropriate rear tab on the pilaster Pull the shelf channel forward and engage
65. information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your lo
66. ingerprints or other residue can affect humidity recovery 12 Reinstall the top section of the humidifier Fasten the latches on each side 13 Reconnect the tubing and wiring to the water bottle as previously Flushing the Humidity Locate the humidity system drain fitting System attached to the inside of the water bottle access door See Figure 7 5 2 Install the hose barb end of this fitting to the supplied tubing and route to a drain or suitable 0 5 gallon 3 8 liter container Latch 3 Connect the other end of the fitting to m rain the quick connect drain located below fitting the fold down storage door Figure 7 2 Figure 7 5 Water Bottle With the incubator turned On allow the entire humidity system to drain This could take several minutes 4 Prepare 0 5 gallon 3 8 liters of decontamination solution per the instructions on the container Sudsy ammonia works well to kill waterborne contaminants such as pseudomonas 5 Disconnect the drain fitting 6 Fill the incubator water bottle with the decontamination solution Change the incubator humidity system setpoint to 9596 Section 2 7 4 7023307 Steri Cult Thermo Scientific Section 7 Routine Maintenance Flushing the Humidity 7 Open both doors to the incubator System continued Tape down the door switch Figure 7 6 on the face of the unit This ensures the humidity nebulizer operates in a 8 seconds on 2 seconds off
67. interruption during the STERILIZATION PHASE Sensor hole plugs are not removed at the end of the cycle Sensor hole plugs are removed but sensors are not reinstalled correctly No action is taken within 10 minutes following REMOVE HEPAs display No action is taken within 5 minutes following REMOVE SENSORS display Then Check for alarms TEMP IS HIGH AIR SENSOR ERROR TEMP CNTRL ERROR A Sterilization Cycle performed on either unit affects performance on second unit Do not use second unit during Sterilization Cycle The Sterilization Cycle may be performed on both units simultane ously with proper electrical connection Unit returns to normal operation SYSTEM OK Unit returns to normal operation SYSTEM OK Hold down Sterilization Cycle initiation button for 3 seconds The cycle will not continue REMOVE SENSORS shows in the dis play If not installed within 5 minutes unit returns to normal opera tion SYSTEM OK An outer door alarm occurs CLOSE DOOR in the display plus an audible cannot be silenced and visual alarm Cycle is cancelled unit goes to CANCELLED COOL PHASE An outer door alarm occurs cannot be silenced HEAT PHASE resumes if the chamber temperature was less than 90 when power was interrupted HEAT PHASE resumes if the chamber temperature was greater than 90 when power was interrupted and the temperature dropped less than 1 C CANCELED COOL PHASE starts if the chamber temp drop is more t
68. k in use goes low the unit automatically switches to the other tank However an audible alarm and warning light activate indicating the low tank condition Pressing Silence clears the alarm condition and does not ringback The warning light on the display remains activated until the tank is replaced and both tanks are normal If the tank not in use goes low the warning light is activated If both banks go low the audible alarm and warning light are activated Thermo Scientific Water Level Alarms Door Open Alarm Thermo Scientific Section 5 Alarms When the water level in the bottle falls to approximately 3 inches 7 6cm a visual alarm with display message WATER LOW occurs Pressing Silence clears the alarm condition and does not ringback The blue backlight on the water bottle flashes until the bottle is filled If the water bottle fill switch is empty low and the humidity reservoir fill switch is empty low an audible alarm with display message WATER EMPTY occurs Pressing Silence mutes the audible alarm but it rings back in 15 minutes The blue backlight on the water bottle flashes until the bottle is filled If the water level inside the humidification system is not as required yet the water level in the bottle is not low then an audible alarm with display message NO WATER FLOW occurs Pressing Silence mutes the audible alarm but it rings back in 15 minutes until the condition is resolved When the doo
69. manual Caution Serial tag amp rating is based on the amperage draw during the sterilization cycle Normal operating amperage is much less Ensure that the electrical circuit will handle the amp draw of the sterilization cycle Warning Connect the incubator to a grounded dedicated circuit The power cord connector is the mains disconnect device for the incubator Position the incubator so the unit can be easily disconnected Plug the provided power cord into the power inlet connector on the back of the cabinet See Figure 1 14 then into the grounded dedicated electrical circuit Open the outer chamber door Open the access door located on the front of the unit below the control board See Figure 1 13 3 Remove the lid of the humidity bottle Latch and fill with sterile distilled water Install pem the lid and close the door being careful not to pinch the tubing Drain fitting Condensation will increase water consumption Under Figure 1 13 Water Bottle normal conditions of 37 C 90 RH 5 02 five door openings per day the water bottle should last 1 1 5 weeks For best operation of the incubator sterilized distilled demineralized or de ionized water should be used in the humidity bottle Water purity should be in the resistance range of 50K to 1M Ohm cm or a conductivity range of 20 0 to 1 0 uS cm Refer to ASTM Standard D5391 93 or D4195 88 for measuring water purity
70. market after 13 August 2005 gt d P gt gt gt EeIII his product is required to comply with the European Union 5 Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo s compliance with this directive the recyclers in your country and information on Thermo Scientific products will be available at www thermoscientific com Always use the proper protective equipment clothing gloves goggles etc VY Always dissipate extreme cold or heat and wear protective clothing v Always follow good hygiene practices v Each individual is responsible for his or her own safety Thermo Scientific 7023307 Steri Cult iii Do You Need Information or Assistance Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermoscientific com Internet Worldwide Web Home Page service led marietta thermofisher com Tech Support Email Address www unitylabservices com Certified Service Web Page Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery
71. mp Controller Failure 5 2 TEMP CNTRL ERROR RH System Fault Sensor Fault Alarms Gas Guard Alarms 7023307 Steri Cult In addition to other safety features designed into Steri Cult incubators a thermostat is provided to monitor the cabinet temperature In the event of a temperature control failure the thermostat will turn off all heaters at a cabinet temperature of 160 C 5 This is intended to be a safety feature to protect the incubator and is not intended to protect the cell cultures or the equipment inside the chamber should a temperature control failure occur Should such a failure occur contact the Technical Services Department or your local distributor This alarm occurs if the humidifier has been detected as being on for an abnormal length of time Possible causes are 1 RH calibrated too low 2 Humidifier tubing pinched or disconnected 3 Access port is plugged 4 Ultrasonic humidifier has heavy build up See Section 6 Cleaning the Humidity System 5 Ifnone of the above contact the Technical Services department The microprocessor in Steri Cult incubators continually scans all available sensors to ensure that they are operating properly Should an error be detected the incubator sounds an alarm and displays the appropriate message Contact the Technical Services Department or your local distributor If the Gas Guard option is installed the choice of tank is available in Configuration mode If the tan
72. n Calibrate is used to calibrate various system parameters Configuration allows for custom setup of various options Thermo Scientific 7023307 Steri Cult 1 3 Section 1 Installation and Start Up Keypad continued The chart below shows the selections under each of the Modes Base Unit Displays are in bold type and Option Displays are in italics e we e Run Settings Calibrate Configure Default Temperature Temp Cal Audible Overtemp C02 Cal New HEPA Timer C02 RH Cal Replace HEPA Reminder RH Access Code Temp Lo Alarm Temp Relay C02 Lo Alarm C02 Hi Alarm C02 Relay RH Lo Alarm RH Hi Alarm RH Relay Tank Select Gas Guard RS485 Address Right and Left Arrows Steps the operator through the parameters of Settings Calibrate and Configuration Modes The right arrow goes to the next parameter the left arrow returns to the previous parameter Up Arrow Increases or toggles the parameter value that has been selected in the Settings Calibrate and Configuration Modes Enter Must press Enter key to save to memory all changed values Down Arrow Decreases or toggles the parameter values that have been selected in the Settings Calibrate and Configuration Modes Silence Key Press to silence the audible alarm See Section 5 for alarm ringback times 1 4 7023307 Steri Cult Thermo Scientific Section 1 Installation and Start Up Displays Message Center Displays the system status Mode at all times Displa
73. n 9 Factory Installed Options Information about connecting to external equipment follows Remote Alarm set of relay contacts is provided to monitor alarms through an RJ11 Contacts telephone style connector on the back of the cabinet Refer to Figure 9 2 for the location of the alarm connector The 12 foot telephone cord P N 190388 and RJ11 to screw terminal conversion box P N 190392 are available through our Services department The remote alarm provides NO normally open output an NC normally closed output and COM common Refer to Figure 9 1 The contacts will trip on a power outage or a temperature controller failure The contacts may also be programmed to trip or not trip on temperature alarms CO alarms and RH alarms See Section 4 Configuration NO NC _ green wire red wire Sov max at 1 A max COM not connected yellow wire black wire HHMH 2 COM 3 N O 4 N C Alarm Contacts Junction Box IMPORTANT USER INFORMATION phone cable to incubator Caution Stored product should be protected alarm contacts RJ 11 connector by an activated alarm system capable of initiating a timely response 24 hours day outputs shown Thermo Alarms provide an interconnect for in alarm condition entralized monitoring Figure 9 1 Remote Alarm Outputs Thermo Scientific 7023307 Steri Cult 9 1 Section 9 Factory Options RS485 Interface Steri Cult incubator models can purc
74. nditioner assembly 230V Temperature sensor assembly 290185 sensor Water cleaning cell for water reservoirs pans and bottles Water cleaning cell quantity of 6 Thermo Scientific Replacing the Fuses Table 11 1 Section 11 Servicing Note See Wiring Diagram 3307 71 page 1 of 2 Fuse Holder Detail 2 a Warning The following procedure requires accessing the electronics area Qualified personnel only should perform this procedure Wearing a electrostatic discharge wrist band or other grounding device is recommended To access the only replaceable fuse in the incubator follow the steps below 1 Turn off the incubator s power switch and disconnect the power cord 2 Remove the four screws from the left side panel Set the panel aside 3 On the left side of the opening is the electronics area Locate the fuse See Table 11 1 below for replacement fuse specifications Replace the blown fuse with a new one 4 Install the panel and secure with the screws 5 Return the unit to service If the fuse blows after restoring power to the incubator contact the Technical Services Department Fuse Specifications Fuse Voltage Manufacturers Part Amperage Rating Rupture Speed Letter Code 115VAC ACC 250VAC ACC 230VAC ACC BUSS GMC 1 0A 1 0 Amp Time Lag T BUSS GMC 740mA 750mAmp Time Lag T BUSS GMC 500mA 0 5 Amp Time Lag T Replacing the CO and RH Air Filters Thermo Scientific Warning The following pr
75. nds until it lights Silence fM Figure 8 1 Sterilization Cycle Initiation Button 3 Enter the access code if applicable An access code is recommended to prevent accidental cycle initiation 4 Pre Sterilization The display toggles between REMOVE HEPAs and PRESS ENTER If no response within 10 minutes the display returns to SYSTEM OR If the filter is removed refer to Section 7 HEPA Filter Maintenance and Enter is pressed within 10 minutes the display begins to toggle between OPEN ACCESS DOOR REMOVE SENSORS and PRESS ENTER If no response within 5 minutes the display returns to SYSTEM 5 Remove the RH and CO sensors To access the sensors open the access door containing the humidity system water bottle The illustration shows the bottle removed for clarity Locate the RH and CO sensors Figure 8 2 Figure 8 2 Hole Plugs and Sensors 6 Pull the sensors outward carefully Allow them to hang See Step 12 for the sensor disinfecting procedure 7 Secured in the same area are sensor hole plugs Install a plug into each sensor location Close the access door 8 After ENTER is pressed with the sensor hole plugs installed the Heat Phase initiates The cycle initiation button flashes and the display toggles between STERILIZING and HEAT PHASE During this period the HEAT light will be on and the cabinet heats to sterilization temperature 702330
76. necting Analog Boards continued 9 4 7023307 Steri Cult To install the analog board Turn the incubator Off and disconnect it from the power source Remove the two screws from the bottom of the left side panel to access the electronics area Using the handle provided lift the side panel up and out On the left side of the opening is the electronics area Locate the Analog Output board Each system monitored Temp CO2 RH requires two conductors Refer to Figure 9 4 Feed the wires through the auxiliary wire through port Figure 9 5 on the back panel This port is a circular fitting adjacent to the remote 53 9 Og C an b B an w w an UUUTUUUU amp 00 2 101 i TEE E Figure 9 4 Conductors on Analog Output Board alarm and RS485 connectors ap Strip the ends of each conductor and wire it to the appropriate terminals of connectors J2 O 9 and or J3 on the analog board Refer to Figure 9 4 SS Auxiliary Wire Through port When wiring is completed install the side panel and secure with the screws Return the Figure 9 5 Through port unit to service Warning can be lethal in high concentrations Refer to warnings in Section 1 of this manual Thermo Scientific 0 Gas Guard Connecting the 0 Gas Supplies De activating the Gas Operati
77. ng from unauthorized unit modifications Any labor costs other than that specified during the parts and labor warranty period which may include additional warranty on C0 sensors blower motors water jackets etc Thermo Scientific 7023307 Steri Cult V Thermo Scientific Section 1 Section 2 Section 3 Table of Contents Installation and 1 1 1 Inventory Management System 1 2 Control Panels Keys Displays amp 1 2 Operation of the Keypad 1 3 Displays RePEc DECRE 1 5 Installing the Incubator 1 5 Stacking the Incubators 1 6 Preliminary Cleaning 1 1 7 Installing the Shelves 1 7 Installing the Access Port Filter Assembly 1 8 Installing the HEPA 1 9 Leveling wa Sa bu e diia dose 1 10 Connecting the Unit to Electrical Power 1 10 Filling the Humidity Water Bottle 1 10 Connecting the Gas Supply 1 11 Humidity and Loading the Chamber 1 12 Incubator Settings 2 1 Setting the Temperature Setpoint
78. nter to save the setting Press the Mode key to return to Run or the right left arrow key to go to next previous parameter 7023307 Steri Cult 4 3 Section 4 Configuration Setting a Low CO Alarm Limit Setting a High CO Alarm Limit 4 4 7023307 Steri Cult The low alarm limit tracking alarm is the deviation from the CO2 setpoint which will cause a low CO alarm The setpoint is variable from 0 5 to 5 096 CO below setpoint The factory setting is 1 0 CO below setpoint minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the Mode key until the Configuration indicator lights 2 Press the right arrow until CO2 LO LMT X X is displayed in the message center 3 Press up down arrow to change the low CO alarm limit 4 Press Enter to save the low CO alarm limit 5 Press the Mode key to return to Run mode or right left to go to next previous parameter The high CO alarm limit tracking alarm is the deviation from the CO setpoint which will cause a high CO alarm The setpoint is variable from 0 596 to 5 096 CO above setpoint The factory setting is 1 096 CO above setpoint When activated the CO valve is disabled 1 Press the Mode key until the Configuration indicator lights 2 Press the right arrow until CO2 HI LMT X X is displayed in the message center 3 Press up down arrow to change the high CO alarm limit 4 Press Enter to save the high C
79. ocedure requires accessing the electronics area Qualified personnel only should perform this procedure using preventive maintenance kit 2270102 Wearing a electrostatic discharge wrist band or other grounding device is recommended A The CO and RH air filters are recommended to be replaced annually Follow the steps below to replace the filters 1 Turn off the incubator s power switch and disconnect the power cord 2 Remove the four screws from the left side panel Set the panel aside 7023307 Steri Cult 11 3 Section 11 Servicing Replacing C0 amp RH On the left side of the opening is the electronics area Locate the CO2 Air Filters continu ed air filter Figure 11 1 Disconnect carefully from the tubing and replace Figure 11 1 CO Air Filter Figure 11 2 RH Air Filter 4 In the right side of the opening is the humidity system Locate the RH air filter in the upper right corner Figure 11 2 Disconnect from the tubing and replace 5 Install the panel and secure with the screws 6 Return the unit to service 11 4 7023307 Steri Cult Thermo Scientific Section 11 Servicing 06 5 0140 93391404 6pg xog 11 5 g 5 10 10 01 S3DNV 3715 INIAVSO 031312345 3SIW33HID 55371 32 83101 ouueur Y N 1NIVd WN 1 1831 18270 3W2S 20 50 6 31 0 ddv 820 0 2 310 NMO VW DW33Hl NOISSIWB3d N3LLIJA INOHLIM 3
80. on of the CO Thermo Scientific Guard Gas Guard Section 9 Factory Options The Steri Cult incubators can be equipped with a built in Gas Guard system P N 1900153 that will operate with a CO gas supply The Gas Guard uses two pressure switches to continuously monitor the pressures of two independent CO supplies and automatically switches from one supply to the other when the supply pressure drops below 10 psig 0 690 bar The Gas Guard s design does not facilitate use by multiple incubators The CO gas supplies must be equipped with two stage pressure regulators with gauges The high pressure gauge at the tank should have a 0 2000 psig range and the low pressure gauge should have a 0 30 psig range The gas supply to the incubator must be maintained at 15 psig 1 034 bar 5 psig Gas pressures below 15 psig cause nuisance alarms to occur on incubators equipped with the built in Gas Guard The inlets for the Gas Guard are located on the back of the cabinet Using 1 4 ID tubing connect one of the CO supply tanks to the fitting labeled CO2 Inlet 1 Tank Connect the second supply tank to the fitting labeled CO2 Inlet 2 Tank Install 3 8 hose clamps to secure the 1 4 ID tubing to the fittings on the cabinet back Refer to Figure 9 6 ES m o C02 Inlet i 1 Tank o 9 The built in Gas Guard is turned ON when i shipped from the factory In addition the Figure 9 6 Inlet Connection
81. or Sterilization Button Figure 1 3 Control Panel Components Thermo Scientific Section 1 Installation and Start Up Control Panel Silence Silences the audible alarm co ntinued Alarm indicator Light pulses on off during an alarm condition in the cabinet Mode select switch Used to select Run Setpoints Calibration and System Configuration Modes Message center Displays system status Mode select indicators Run Run Menu Settings Set Points Menu Calibrate Calibration Menu Configuration Configuration Menu Up down arrows Increments number values toggles between choices Enter Stores the value into computer memory Heat indicator Lights when power is applied to the heaters Heat display Displays temperature C Humidity indicator Lights when humidity is required RH display Displays humidity Left and right arrows Moves the operator through the choices of the selected mode Inject indicator Lights when CO is being injected into the incubator chamber display Displays 96 Sterilization button Starts Sterilization Cycle i e Steri Cult incubator has four basic modes whic ow incubator Operation of the The Steri Cult incubator has four b des which all b Keyp ad setup Run Settings Calibrate and System Configuration Run is the default mode which the incubator will normally be in during operation Settings is used to enter system setpoints for incubator operatio
82. put board P N 191761 191762 191763 is an option that allows the incubator to output analog signals representing the air temperature of the cabinet CO content and relative humidity depending upon which systems are in the incubator There are three different analog output board options available 0 1V 0 5V or 4 20mA signals Negative display readings will output The outputs do not have isolated grounds Refer to Table 9 1 for output specifications of the three boards For the 0 1V and 0 5V boards the recording device must supply a load gt 1000 ohm For the 4 20mA board the recorder must supply a load of lt 100 ohm To wire in the analog output board use a shielded 22 gauge 3 conductor wire P N 73041 maximum length 50 ft 15 2m This wire is readily available from other vendors including Alpha P N 2403 and Deerborn P N 972203 Caution Accuracy of the output at the board terminal strip to the incubator display is 1 unit There is no calibration from the incubator Calibration to the incubator display must be at the instrument connected to the output board Table 9 1 Analog Output Board Specifications 191761 4 20 mA 191763 0 1V 191762 0 5V Output Scaling Output Scaling Output Scaling 4 20mA Equals 0 1 V Equals 0 5V Equals Temperature 12 5 162 5 C 0 0 150 0 C 0 0 150 0 C RH 0 100 RH 0 100 RH 0 100 RH C02 0 0 100 0 9602 0 100 0 02 0 100 0 02 7023307 Steri Cult 9 3 Section 9 Factory Options Con
83. r is opened on the Steri Cult incubator heat and CO2 injection are disabled The door must be securely latched for heat and CO2 injection to resume after a door opening If the door is latched yet the display still shows Door Ajar the door switch could be faulty Call Technical Services 7023307 Steri Cult 5 3 Table 6 1 Troubleshooting Guide Problem Poor temperature recovery and or temperature overshoot Poor CO recovery and or actual higher than display Poor RH recovery Condensation on cabinet face Condensation on cabinet interior Thermo Scientific Section6 Troubleshooting Certain conditions can occur in usage of this incubator which can be resolved by checking the troublshooting chart below If the issue is not listed or cannot be resolved contact the Services department Possible Cause HEPA filter is dirty Temperature probe not reinstalled correctly 02 sensor not reinstalled correctly Access port is plugged Humidifier tubing pinched or disconnected Humidifier has heavy build up RH sensor not reinstalled correctly Removable gasket not reinstalled properly RH out of calibration RH sensor not reinstalled correctly Solution If the HEPA filter is older than six months replace with new Ensure temperature probe holder bottoms out in hole Ensure C02 sensor bottoms out in hole Install access port filter assembly If it is older than six months replace with new
84. rom the humidity setpoint which causes a high RH alarm The setpoint is variable Alarm Limit from 5 to 20 RH The factory setting is 1096 RH When the alarm is activated the humidity system is disabled 1 25 Press the Mode key until the Configuration indicator lights Press the right arrow until RH HI LMT XX is displayed in the message center Press up down arrow to change the RH high alarm limit Press Enter to save the RH high alarm limit Press the Mode key to return to Run mode or right left to go to next previous parameter Enabling RH Alarms The high and low RH alarms can be programmed to trip the remote alarm to Trip Contacts ncs A setting of ON activates this ability A setting of OFF will not 0 P allow the RH alarm to trip the contacts The factory setting is ON 1 2 46 7023307 Steri Cult Press the Mode key until the Configuration indicator lights Press the right arrow until RH RELAY ON OFF is displayed in the message center Press up down arrow to toggle the setting ON or OFF Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter Thermo Scientific Section 4 Configuration Selecting a Prima FY units equipped with the Gas Guard option a primary tank can be Tank selected The primary tank will be either Tank 1 or 2 The factory setting is Tankl Press the Mode key until the Configuration indicator lights Pre
85. s Tank Select for the Gas Guard is specified as Tank 1 when shipped Refer to Section 4 Configuration to de activate the Gas Guard or change the Tank Select from 1 to 2 If the Gas Guard system is de activated the incubator functions normally by supplying from the supply tank connected to Inlet 1 With the Gas Guard in operation the incubator uses the gas supplied by the tank connected to Inlet 1 until the pressure drops below 10 psig 0 690 bars At this time the Gas Guard automatically switches to the gas supplied through CO2 Inlet 2 In addition the incubator automatically changes the Tank Select in Configuration mode from 1 to 2 to indicate that the incubator is now using gas supplied through Inlet 2 If the gas supply to Inlet 1 is replenished the incubator will continue to operate using the gas supplied 7023307 Steri Cult 9 5 Section 9 Factory Options Operation of the CO Gas Guard continued Dataloggger 9 6 7023307 Steri Cult through Inlet 2 unless the operator changes the Tank Select from 2 to 1 through Configuration mode Refer to Section 4 Configuration Audible and visual alarms occur on the control panel when the gas guard switches from one supply to the other The audible alarm sounds until the operator presses the Silence key on the control panel The visual alarm in the Message Center reads Tank 1 Low while the audible alarm is sounding but the message is removed when the operator
86. ss the right arrow until TANK SELECT X is displayed in the message center Press up down arrow to toggle setting between 1 and 2 Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter Disabling the Gas os units equipped with the Gas Guard option the Gas Guard system Guard System may be turned ON or OFF if it is not in use The factory setting is ON 2 Thermo Scientific Press the Mode key until the Configuration indicator lights Press the right arrow until GAS GUARD ON OFF is displayed in the message center Press up down arrow to toggle the setting ON or OFF Press Enter to save the setting Press the Mode key to return to Run mode or right left to go to next previous parameter 7023307 Steri Cult 4 7 Section 4 Configuration Setting a RS485 Communications Address 4 8 7023307 Steri Cult On units that have the RS485 option direct communication with the Model 1535 alarm system can be established Each piece of equipment connected to the Model 1535 must have a unique address An address of 0 24 can be entered for the incubator A setting of 0 is an invalid address that the 1535 will ignore The factory setting for the RS485 address is 0 1 2 Press the Mode key until the Configuration indicator lights Press the right arrow until 485 ADDRESS XX is displayed in the message center Press up down arrow to move the RS485 address
87. te OPTIONAL RS 485 OUTPUT KIT 1900152 OPTIONAL GAS GUARD KIT 1800153 C02 PRESSURE SWITCH 1 TANK EMPTY 360213 PRESSURE SWITCH 2 TANK EMPTY 360213 SOLENOID 3 WAY 12 0 250121 OPTIONAL ANALOG OUTPUT KITS 191761 4 to 20mA 191762 0 to SVDC 191763 0 to IVDC Electrical Schematic Model 3308 amp 3311 Steri Cult II Incubator 3308 70 0 D Rev 11 Page 2 of 3 7023307 Steri Cult Thermo Scientific Section 11 Servicing 77 WIRE REFERENCE CHART GA cum GA cum GA 78 te 24 20 purple 4 22 GREEN 6 2 20 mu 5 22 RED 79 16 26 20 51 22 GREEN 44 18 BLACK 27 20 veo 52 22 Back 80 20 2 20 53 2473 BLACK sa 18 w 20 Bak 54 24 3 RED 8l 20 w 22 BRON 55 24 3 WHITE 20 prow 3 22 56 24 2 RED 82 7 20 2 22 57 24 2 BLACK 2 purple 22 vun 58 20 83 g 20 vui 22 5 20 20 5 22 mus 5 20 84 1 20 3 22 20
88. the channel s front slot into the pilaster s appropriate forward tab Refer to Figure 1 8 Side duct Side duct tab tab Shelf channel rear slot L gt FZ Shelf channel FRONT front slot Figure 1 8 Slots and Tabs Thermo Scientific 7023307 Steri Cult 1 7 Section 1 Installation and Start Up Installing the Shelves 4 Figure 1 9 shows a shelf channel installed on pilasters on the right side continued of the chamber 0207 2 n Ew 5 Ky 2222002020008 0 Figure 1 9 Installed Shelf Channel 5 Slide the shelves in side flanges upward along the channels into the chamber Installing the Access Port 1 Locate the opening in the top left corner on the inside of the chamber Filter Assembly Remove the tape covering the opening on the outside of the unit 2 Install the the filter stopper assembly into the opening inside the chamber See Figure 1 10 Caution Filter MUST be installed inside the chamber or it will become plugged with condensation and inhibit the recovery of the RH system Do not replace this filter with a solid cover of any kind inner chamber Figure 1 10 Access Port Filter Installation 1 8 7023307 Steri Cult Thermo Scientific Installing the HEPA Filter Thermo Scientific Section 1 Installation and Start Up Caution Use care when handling the filter T
89. the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage pro tection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial lab oratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply Pollution Degree describes the amount of conductive pollution present in the operating environment Pollution Degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Thermo Scientific Thermo Scientific Parts List Section 11 Servicing Service and maintenance information follow Part Number 2270102 3310902 3310903 1900446 1900447 3310904 3307904 191682 230201 230135 230120 156125 191681 290185 1900160 224310 224307 231204 250087 290174 230180 400201 360213 250121 Description Preventive maintenance kit door gasket HEPA filter CO2 air filter RH bacterial filter access port filter water bottle air filter Pilaster single Shelf channel right or left Pilaster 4 pack Shelf channel 10 pack Diffuser pan large 3310 3311 Diffuser pan small 3307 3308 Microboard main control Fuse 750mA TD 5 x 20mm humidity system 250V F
90. tpoint 1 Press the Mode key until the Settings indicator lights 2 Press the right arrow until OVERTEMP XX XC is displayed in the message center 3 Press the up or down arrow key until the desired Overtemp setpoint is displayed 4 Press Enter to save the setting 5 Press the Mode key until the Run indicator lights or press the right or left arrow to go to the next or previous parameter Thermo Scientific Setting the CO Setpoint Setting the Relative Humidity RH Setpoint Thermo Scientific Section 2 Incubator Settings The setpoint range is 0 0 to 20 0 settable to within 0 1 CO The incubator is shipped from the factory with a CO setpoint of 0 0 At this setting all CO2 control and alarms are turned off To change the CO setpoint 1 Press the Mode key until the Settings indicator lights 2 Press the right arrow until CO2 XX X is displayed in the message center 3 Press the up down arrows until the desired CO setpoint is displayed 4 Press Enter to save the setpoint 5 Press the Mode key until the Run indicator lights to go to Run mode or press the right left arrow keys to go to next previous parameter The RH setpoint range is 0 to 95 settable to within 1 RH Settings above 90 may cause some condensation to occur in the chamber The incubator is shipped from the factory with an RH setpoint of 0 At this setting all RH control water level control and alarms are turned off
91. use 1A TD 5 x 20mm accessory outlet 115V Fuse TD 5 x 20mm accessory outlet 230V Motor hot air 115VAC 50 60 Hz Display board Probe 2252 Ohm 25 C 0 156 x 2 HEPA filter kit includes 760207 amp 1900185 Stainless steel shelf large Stainless steel shelf small Infrared type C02 sensor 02 valve kit with assorted fittings RH sensor assembly 1ft 12A DPDT circuit breaker switch power Switcher kit 40W Pressure switch Gas Guard option Solenoid gas valve 12VDC Gas Guard option 7023307 Steri Cult 11 1 Section 11 Servicing Parts List Par Number continued 06 1900161 250049 250128 360251 360263 280096 100119 139033 360171 360255 900140 130625 105070 247013 760208 1900199 1900200 1900201 1900202 1900233 50109579 130101 11 2 7023307 Steri Cult Description Leveling foot VOC HEPA filter kit includes 760208 amp 1900185 12V valve water fill 12V valve RH air valve Door switch outer Door switch inner Blue LED light source 3 5 x 1 5 CCW blower wheel Humidifier humidity system Liquid level switch float Liquid level switch protection float 1 6 sq 12VDC fan x CFM Tubeaxial fan 31 CFM 115V Fan Inner glass door Humidity system drain fitting HEPA VOC filter only Complete blower assembly includes motor wheel scroll mounting materials Complete humidifier assembly includes nebulizer and housing RH conditioner assembly 115V RH co
92. www turck com thermoscientific com 2014 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Thermo Fisher Scientific 401 Millcreek Road Marietta Ohio 45750 SCIENTIFIC A Thermo Fisher Scientific Brand
93. ys CLASS 100 SYSTEM OK or RH WAITS ON TEMP see Setting the RH Setpoint for further details during normal operation Alarm messages display if the system detects an alarm condition See Section 5 Alarms The display message CLASS 100 is a timing mechanism indicating that under normal operating conditions with the HEPA filter installed the air inside the chamber meets the Class 100 air cleanliness standard for particulates of 0 5 micron size or larger per cubic foot of air For further information on the Class 100 classification of air quality see Appendix A 3 Upper Displays The first upper display shows the temperature The second display shows the percentage of CO The third display shows the humidity percentage Installing the Caution Single and stacked units must be installed against a wall or similar Incubator structure l Maintain a three inch clearance behind the incubator for electrical and gas hook ups In addition a three inch ventilation space is needed on each side of the unit 2 Locate the unit on a firm level surface capable of supporting the unit s weight 340 Ibs Models 3307 amp 3308 410 lbs Models 3310 amp 3311 3 Locate the unit away from doors and windows and heating and air conditioning ducts Caution Lift the unit only by the sides of the cabinet base Do not attempt to lift by the front and back This places stress on the outer door hinges A Thermo Scientific 7023307 Steri Cult 1 5 Section

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