Q500 - Qsonica, LLC.
Contents
1. ey TIMER Used with the numeric keys to set the duration of ultrasonic key application from 1second to 9 hours 59 minutes 59 seconds PULSER Used with the numeric keys to set the pulse mode The ON cycle and ke OFF cycle can be set independently from 1 second to 59 seconds y Red indicator lights when pulser is in the OFF portion of the cycle START STOP Starts or stops the ultrasonics In the STOP mode the red indicator key goes off l Switches the main power on key 0 Switches the main power off key AMPL Controls the amplitude of vibration at the probe tip Used with the AMPL key when the unit is on stand by to set the amplitude of vibration at the probe tip Also used to increase or A yY decrease the amplitude in small increments while the unit is running key To accomplish this task depress the ENTER REVIEW key twice to display AMPLITUDE CONTROL then depress the A or VW key as required 11 Rev 10 13 REAR PANEL 9 pin D sub connector Connects to external actuation device and enable power and IO Port frequency monitoring Footswitch Connector Connects to the footswitch cable Power Supply Connector Connects to the electrical line cord and encases the fuse s 9 PIN D SUB CONNECTOR Description 1 Not connected 2 Not connected 3 Not connected 4 5 Enables connection to an external power monitor 5 mv 1 watt 6 Ground 7 Energizes the ultrasonics when connected t2. appropriate probe for your application Small volumes require a small tip to fit inside the sample tube Small tips aka Microtips are recommended for processing samples inside small thin vessels and never samples larger than 50ml Microtips are high intensity and made for short processing times Using a microtip for long time periods will generate a considerable amount of heat Microtips should be used in pulse mode to reduce heat buildup Larger volumes require a larger probe for effective processing For example a 1 probe will process 1 liter much faster than a probe Using the proper size probe will not only reduce the processing time but increase the lifespan of the probe The addition of a stir bar can greatly aid processing of large samples A probe should not be used to process a volume larger than indicated on the chart unless the application is reviewed and approved by a Qsonica representative While there is no absolute sample volume range for any probe horn below is a general guideline to follow Using a sample volume outside each tip diameter s range is generally not recommended 1 16 2mm 200ul 2ml 1 8 3mm 1ml 15ml 1 4 6mm 10ml 50ml 1 2 12mm 20ml 250ml 3 4 19mm 100ml 500ml n 25mm 200ml 1 000ml 1 with booster 750ml 1 500ml Vessel shape and size A narrow vessel is preferable to a wide vessel The ultrasonic energy is generated from the tip and is directed do
3. in an area that is free from excessive dust dirt explosive and corrosive fumes and extremes of temperature and humidity If processing flammable liquids use an approved fume hood and do not place the power supply in the fume hood When positioning the unit be sure to leave adequate space behind the unit so that all connections can be easily disconnected 1 3 Rev 10 13 7 Operating Instructions CAUTION Do not operate the power supply unless it is connected to the converter Never allow liquid to spill into the converter Do not allow a Microtip to vibrate in air Do not allow the vibrating Microtip to contact anything but the sample Never place a washer between the converter probe or horn Never apply grease to the mating surfaces or threads of the converter probe or Microtip Should it become necessary to remove a probe use the wrenches supplied Never attempt to remove the probe by twisting the converter housing or holding it in a vice as this may damage the electrical connections within the housing CAUTION LOW SURFACE TENSION LIQUIDS ORGANIC SOLVENTS The probes solid or with a replaceable tip are tuned elements that resonate at a specific frequency If the replaceable tip is removed or isolated from the rest of the probe the element will no longer resonate at that frequency and the power supply will fail Unlike aqueous water based solutions which rarely cause problems solvents and low surface tension liq
4. related to the amplitude of the radiating face of the tip or horn It is amplitude that must be provided maintained and monitored The unit provides controlled amplitude under varying load conditions in order to give reproducible results 9 Rev 10 13 5 Description of Components Functions of Controls The Model Q500 includes a standard 1 2 diameter probe 4220 Q500 Front Panel Converter Cable Converter Generator 2 Horn with replaceable tip Q500 Rear Panel 9 Pin Connector 0 HAHHHHHHHHHHHHHHHHHAA a Cooling Fan Essessoseseessesaoona Rsseseesessesseszoosa s Footswitch P THEE BERE Baotianin O my Temperature Converter Probe Connector Cable O Connector o e Power Cord o o Connector 10 Rev 10 13 FUNCTIONS OF KEYS CONTROLS INDICATORS AND CONNECTORS FRONT PANEL Displays prompts and the following control parameters e Amplitude selected e Output power delivered to the probe in watts and as percentage of the total power LCD display Selected duration of processing Actual processing time e Elapsed time e Set and read temperature e Pulse duration Accumulated amount of energy in Joules delivered to the probe 0 9 key Input digits Clears the preceding entry ey ENTER Enters data into the program and selects various parameters for REVIEW k display on the LCD display
5. the sample to foam Processing at a lower intensity setting without foam is more effective than processing the sample with foam as ultrasonic cavitation will be diminished Decreasing the intensity setting increasing the processing time and lowering the sample temperature will usually prevent foaming Also ensure that the probe tip is not touching the wall of the sample vessel as it may be damaged and it will not vibrate properly The Ultrasonic Processor is now ready for continuous operation To energize the ultrasonics press the START key or the footswitch To de energize the ultrasonics press the STOP key or release the footswitch If the Time or Pulse functions must be used refer to the appropriate paragraphs below Note The START key and footswitch are mutually exclusive If the process is initiated by the START key the footswitch becomes inoperative If the process is initiated by the footswitch the STOP key becomes inoperative To increase or decrease the amplitude in small increments when the ultrasonics is on depress the AMPL to display Amplitude Setting on the screen then depress the A or W key as required Since the amplitude required is application dependent and subject to the volume and composition of the sample it is recommended that the amplitude be selected through experimentation by increasing or decreasing the level of intensity as needed to properly process the sample to achieve desired results Be sure to use t
6. 0 amplitude will require approximately 5 watts to operate in air The amplitude of this probe is approximately 120um Insert the probe in water and the wattage reading will increase to approximately 90 watts The wattage required to operate the probe will increase as the load increases but the amplitude remains the same The AMPLITUDE control allows the ultrasonic vibrations at the probe tip to be set to any desired level Although the degree of cavitation ultrasonic energy required to process the sample can readily be determined by visual observation the amount of power required cannot be predetermined A sensing network continuously monitors the output requirements and automatically adjusts the power to maintain the amplitude at the preselected level The greater the resistance to the movement of the probe due to higher viscosity deeper immersion of the probe into the sample larger probe diameter or higher pressure the greater the amount of power that will be delivered to the probe Setting the AMPLITUDE control to its maximum will not cause the maximum power rating of the unit to be delivered to the sample The maximum power 500 watts that the Ultrasonic Processor is capable of delivering will only be delivered when the resistance to the movement of the probe is high enough to draw maximum wattage It is the intensity of cavitation that measures the effectiveness of the sonication not the total power applied to the system Intensity is directly
7. CYSONICA Sonicator ULTRASONIC PROCESSOR Part No Q500 OPERATION MANUAL Qsonica LL 53 Church vil Rd Ne i si oi 06470 USA 203 426 0101 a 208 di info Rev 10 13 10 11 12 Table of Contents Section Warranty Warnings Specifications Principles of Operation Description of Components Functions of Controls Preparation for Use Operating Instructions Maintenance Troubleshooting Service Return of Equipment Service Safety Certification form Addendum Page No 10 12 13 14 18 19 21 22 22 23 24 26 Rev 10 13 1 Warranty Your Ultrasonic Processor is warranted and backed by the manufacturer for a period of two years from the date of shipment against defects in material and workmanship under normal use as described in this instruction manual During the warranty period the manufacturer will at its option as the exclusive remedy either repair or replace without charge for material and labor the part s which prove to be defective provided the unit is returned to us properly packed with all transportation charges prepaid Ultrasonic probes are guaranteed against defects for a period of one year from date of shipment A defective probe will be replaced once without charge if failure occurs within the warranty period Wear resulting from cavitation erosion is a normal consequence of ultrasonic processing and is not covered by this warranty The manufacturer neither assumes nor authori
8. al AC socket adapter must be added Important Universal adapters do not convert voltage or frequency Manufacturer is not responsible for damage caused by the use of an improper power cord or adapter Transformers are not recommended WEEE Statement This product contains electrical or electronic materials The presence of these materials may if not disposed of properly have potential adverse effects on the environment and human health Presence of this label on the product means it should not be disposed of as unsorted waste and must be collected separately As a consumer you are responsible for ensuring that this product is disposed of properly To find out how to properly dispose of this product contact Customer Service 7 Rev 10 13 4 Principles of Operation The ultrasonic electronic generator transforms AC line power to a 20 KHz signal that drives a piezoelectric converter transducer This electrical signal is converted by the transducer to a mechanical vibration due to the characteristics of the internal piezoelectric crystals The vibration is amplified and transmitted down the length of the horn probe where the tip longitudinally expands and contracts The distance the tip travels is dependent on the amplitude selected by the user through the touch screen pad As you increase the amplitude setting the sonication intensity will increase within your sample In liquid the rapid vibration of the tip causes cavitation the forma
9. e Microtip or extender in its place Microtips should be used in pulse mode to prevent overheating which could potentially crack the tip Contact us if you have questions regarding using a microtip Horns and probe tips wear after normal usage Using a severely worn probe tip can damage internal generator components 14 Rev 10 13 9 Ifusing a laboratory stand mount the convertor probe assembly using a clamp Be sure to secure the clamp to the upper section of the convertor housing only Never secure the clamp to any other portion of the convertor probe assembly If you are using an acoustic enclosure mount the convertor properly in the convertor collar 10 Connect the converter cable to the power supply and then to the top of the convertor Push the connectors in and turn the chrome rings clockwise 1 4 turn to secure the connectors 11 If application or procedure instructs that the ultrasonic processor be run for a period of time that will allow the probe convertor assembly to become warm to the touch it is recommended that the convertor be air cooled with dry compressed air Never run the convertor and horn assembly if it becomes hot Connect either one of the air fittings on top of the converter to a source of clean dry compressed air This will cool the convertor so that it can function normally under load for an extended duration without becoming too hot Note Overheating will damage the converter See addendum for converter coolin
10. ectric shock Hazardous voltage A Caution Risk of danger Refer to User Manual 5 Rev 10 13 3 Specifications Generator Input Voltage 100 VAC 120 VAC 50 60 Hz 220 VAC 240 VAC 50 60 Hz Fuse Rating 15 Amps slo blo 8 Amps slo blo oon 15 Ibs 6 8 Kg Dimensions 8 W x 15 25 L x 8 5 H 203 mm x 387 mm x 216 mm Output Voltage 1000 V rms max Output Frequency 20 KHz Weight 2 Ibs 900 g Dimensions 7 25 L x 2 5 Dia 183 mm x 63 5 mm Standard 1 2 Horn Weight 0 75 Ibs 340 g Dimensions 5 375 L x 5 Dia 136 mm x 13 mm 6 Rev 10 13 Environmental Pollution Degree 2 Installation Category Operating Limits Shipping Storage Temperature 41 104 F 5 40 C Relative Humidity 10 95 Non Condensing Altitude 6 651 ft 2000 m Temperature 35 120 F 2 49 C Relative Humidity 10 95 Non Condensing Ambient Pressure Extremes 40 000 ft 12 192 m Restriction of Hazardous Substances ROHS Relative humidity RoHS Compliant Directive 2002 95 EC Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity to 40 C Other For indoor use only Only use IEC approved Fast acting fuses Cooper Bussman series S500 The Power Cord supplied with the ultrasonic processor must be used If the 220V plug is not configured to match the wall receptacle a properly grounded univers
11. ed cruise control on an automobile can to a certain extent be compared to an Ultrasonic Processor The speed cruise control is designed to ensure that the vehicle maintains a constant rate of travel As the terrain elevations change so do the power requirements The cruise control senses these requirements and automatically adjusts the amount of power delivered by the engine in order to compensate for these ever changing conditions The greater the terrain rate of incline and greater the resistance to the movement of the vehicle the greater the amount of power that will be delivered by the engine to overcome that resistance and maintain a constant speed The ultrasonic processor was designed to deliver constant amplitude to your liquid sample regardless of these changes in load much like the vehicle s cruise control described above As a liquid is processed the load on the probe will vary due to changes in the liquid sample i e viscosity concentration temperature etc As the resistance to the movement of the probe increases increased load on the probe additional power will be delivered by the power supply to ensure that the excursion at the probe tip remains constant The displayed wattage readings will 8 Rev 10 13 vary as the load changes however the amplitude will remain the same The resistance to the movement of the probe determines how much power will be delivered to maintain amplitude For example a 1 2 probe at 10
12. g enclosure or ear protection be used when operating the Ultrasonic Processor m NEVER immerse the converter in liquids of any kind or let condensed moisture or liquid drip into the converter m NEVER grasp an activated horn or touch the tip of a vibrating probe It can cause severe burns tissue damage m NEVER allow a microtip to vibrate in air m NEVER hold or clamp the converter by the front driver or by the horn itself This can cause permanent damage to the system Support the converter by only clamping around the converter housing upper portion E f needed air cool the convertor with dry compressed air m Do NOT allow the tip of a vibrating horn or probe to touch the counter top or any other hard surface It could damage the probe overload the generator or damage the surface Bi Avoid touching the bottom or sides of a glass or plastic container with an activated probe It could crack or shatter the glass or melt the plastic m Turn OFF the power switch unplug the generator and disconnect the power cord from the back of the generator before attempting to replace the fuses M Inspect high frequency cable for cracks in the protective outer jacket Mm Do not operate unit with a damaged cable Doing so may cause serious injury m Incase of AC power loss wait 3 minutes minimum before reapplying power m Do not turn off AC mains power while running a horn Stop sonication prior to removing AC power Symbols Caution Risk of el
13. g instructions Operation 1 Press the ON key The screen will display the power rating and frequency of the Ultrasonic Processor and the following control parameters Time Pulse AMPLITUDE Desired amplitude must be set in order for the Ultrasonic Processor to be operational The other control parameters Time and Pulse do not have to be set for continuous operation AMPL displays the percentage of amplitude that was previously selected To set the amplitude at 40 when the ultrasonics is off press the AMPL key and the numeric keys for a 40 reading on the screen and then press the ENTER REVIEW key Pressing the AMPL key and the A or key for a reading of 40 and then pressing the ENTER REVIEW key will also achieve the same result Note The minimum amplitude setting is 20 The screen will display Time Pulse Ampl 40 Warning Microtip Amplitude Setting Limits When working with a Microtip do not operate the equipment beyond the maximum amplitude limits listed below Ignoring this caution will cause the Microtip to fracture Size Maximum Amplitude Tapered Microtip 1 16 2mm 40 1 8 3mm 40 1 4 6mm 75 1 5 Rev 10 13 Immerse the probe tip into the sample liquid Ensure that the probe is immersed to a depth that is at least 1 5 times the tip diameter If the probe is immersed to an insufficient depth air will be injected into the sample causing
14. have a direct relationship If you operate at a low amplitude setting you will deliver low intensity sonication If you operate at a high amplitude setting you will have high intensity sonication In order to be able to reproduce results the amplitude setting temperature viscosity and volume of the sample are all parameters that need to remain consistent The amplitude not the power is most critical when trying to reproduce sonication results Power has a variable relationship with amplitude intensity For example sonicating water requires less wattage when compared to a viscous sample such as honey While sonicating both samples at the same amplitude setting the power wattage will differ because the viscous sample will require more watts in order to drive the horn The viscous sample puts a heavier load on the probe so they system must work harder to vibrate up and down at the same amplitude setting The honey may draw double the watts when operated at the same amplitude as the water sample Small fluctuation in the wattage display during sonication is normal Major swings in wattage 30 watts may indicate a problem with the sample setup or the sonicator itself 26 Rev 10 13
15. he fittings provided for the top of the convertor for air cooling if necessary Circulate clean dry compressed air through the convertor to cool the convertor during use TIMER In the pulsed mode the processing time will be different from the elapsed time because the processing time function monitors and controls only the ON portion of the duty cycle For example for 1 hour processing time the elapsed time will be 2 hours if the ON and OFF cycle are set for 1 second To set the processing time press the TIMER key The screen will display Time Setting Hrs Min Sec Using the numeric keys set the processing time as required for example Time Setting Hrs 5 Min 30 Sec 25 Press the ENTER REVIEW key The screen will display 1 6 Rev 10 13 Time 5 30 25 Pulse Ampl 40 PULSER By inhibiting heat build up in the sample the pulse function enables safe treatment of temperature sensitive samples at high intensity The ON and OFF pulse duration can be set independently from 1 second to 59 seconds During the OFF portion of the cycle the red indicator on the PULSE key will illuminate If the OFF portion of the cycle exceeds two seconds a cautionary message Sonics in OFF Cycle will warn the operator against touching the ultrasonic probe 1 Tosetthe pulser press PULSE key The screen will display Pulse on _ _ sec Pulse off _ _sec 2 Using the numeric keys set the ON portion of the cycle
16. it unplugged from the AC power source Long exposure to acids or caustics results in corrosion of metal parts or components Check the generator converter and cables periodically for any signs of rust or discoloration If discoloration is found move the ultrasonic processor away from the source of the contaminant Examine the condition of the high voltage cable that attaches the converter to the generator Inspect the wire insulation for damage such as wear burning from hot plate contact or breakage from extended use or rough handling In general use the cable assembly should not be used to carry the converter or pull it toward the user Make certain the cable always has slack and is never tensioned If necessary move the generator or converter assembly closer to one another to accomplish this If this is not possible contact your Customer Service Representative to obtain a longer cable WARNING Do not use a cable with broken end connections exposed wires or frayed insulation High voltage is present in the cable and will pose a shock hazard Do not touch the converter assembly until the power switch is off and the unit is unplugged Microtip Probe Maintenance Ultrasonic processors create high intensity vibration which puts stress on the converter and horn assembly The sides and end of the probe must never be allowed to come in contact with anything but the solution When using a microtip the stress resulting at the point of contact with
17. o ground 8 and 9 Enables the intensity to be remotely adjusted using an external 10k potentiometer NOTE To vary the to pin intensity remotely using 10K toping a variable DC power supply 0 5V instead of to pin 6 a 10 K potentiometer connect positive to pin 8 and negative to pin 6 12 Rev 10 13 6 Preparation for Use INSPECTION Prior to installing the ultrasonic processor perform a visual inspection to detect any evidence of damage which might have occurred during shipment Before disposing of any packaging material check it carefully for small items The ultrasonic processor was carefully packed and thoroughly inspected before leaving our factory The carrier upon acceptance of the shipment assumed responsibility for its safe delivery Claims for loss or damage sustained in transit must be submitted to the carrier If damage has occurred contact your carrier within 48 hours of the delivery date DO NOT OPERATE DAMAGED EQUIPMENT Retain all packing materials for future shipment ELECTRICAL REQUIREMENTS The Ultrasonic Processor requires a fused single phase 3 terminal grounding type electrical outlet For power requirements check the label on the back of the unit For your personal safety do not under any circumstances defeat the grounding feature of the power cord by removing the grounding prong A WARNING INSTALLING THE ULTRASONIC PROCESSOR The ultrasonic processor should be installed
18. of ___The equipment was used in conjunction with or exposed to radiological biological or chemical agents and has been decontaminated rendering it safer for handling use or disposal Authorization By accepting authorization to return the equipment listed above the undersigned assumes all responsibility and liability for radiological biological and chemical decontamination Delivery of the equipment can be refused if necessary documentation is not provided or where it is determined that the equipment has not been properly decontaminated If it is determined that the equipment was not properly decontaminated the Authorized Repair Facility reserves the right to bill the customer for any and all costs associated with the decontamination and or appropriate disposal of the equipment In the event the equipment has been exposed to radiological contamination the signature of the Radioactive Safety Officer is required Print name RA Signature Date 23 Rev 10 13 Addendum Converter Cooling Continuous sonication will cause both the probe and sample temperature to increase The heat will transfer up to the converter If the converter overheats the internal crystals can crack and the entire converter will require replacement Converter damage due to overheating is not covered under warranty Cooling the sample buy submerging the beaker or tube in ice will help to cool the probe and converter Chillers are also commonly used If you ha
19. re made from titanium and can be autoclaved the converter is an electrical part and cannot be sterilized in this manner Before each procedure place the probe tip in water or alcohol and turn the power on for a few seconds to remove residue The tip also can be sterilized using alcohol with the power on 21 Rev 10 13 9 Troubleshooting Your Ultrasonic Processor was designed to provide you with years of safe and dependable service Nevertheless because of component failure or improper usage the possibility does exist that it might not perform as it should shut down or stop working all together The most probable causes for malfunction are listed below and should be investigated 1 A connectoror cable is damaged 2 The unit was plugged into an electrical outlet that provides a different voltage from that required See Electrical Requirements 3 The horn probe booster or microtip is not tightened properly with the wrenches provided Remove clean and re tighten all probes tips The convertor and or microtip has been dropped A microtip being operated is damaged or worn past its useful life A fuse s has failed SOT OVERLOAD CONDITION If the Ultrasonic Processor stops working and an OVERLOAD indication is displayed on the screen check for possible causes as outlined in the above paragraph Then press the OFF key to switch the unit off and the ON key to switch the unit back on to restart the equipment If the problem persi
20. sts after reviewing each of the 6 items above please contact Customer Service 10 Return of Equipment It is suggested that an Ultrasonic Processor in need of repair be sent back to the factory In order to receive prompt service always contact your Customer Service Representative before returning any instrument Include date of purchase model number and serial number Please obtain a Return Authorization Number prior to returning the instrument Care should be exercised to provide adequate packing to insure against possible damage in shipment The Ultrasonic Processor should be sent to the Service Department with all transportation charges prepaid and return of shipment indicated Important The user must certify that the ultrasonic processor and or the accessories returned for repair are free of any biohazardous or radioactive material and are safe for handling Please complete the Safety certification form on the next page and send it in with your equipment Do not return any equipment unless such a certification can be made 22 Rev 10 13 SAFETY CERTIFICATION FORM Items being returned Please check only one item below ____ The equipment was never used or exposed to any radiological biological or chemical agents and is safe to handle use or dispose of ____ The equipment was used but not in conjunction with or exposed to any radiological geological or chemical agents and is safe to handle use or dispose
21. th the system 1 9 Rev 10 13 2 Clean threaded stud Use alcohol and a cotton swab to remove any debris on the threading of the connecting stud Allow the alcohol to dry completely 3 Clean threading in converter Hold the convertor horizontally and use alcohol and a cotton swab to remove any debris on the threading Do not allow liquid to drip into Convertor Allow the alcohol to dry completely 4 Reattach probe to convertor Screw the probe back onto the convertor by hand and tighten with the wrench set A tight connection is required for safe operation 5 The tips on replaceable tip probes can be removed for cleaning and or replacement Use alcohol and a cotton swab to remove any debris on the threading of the tip or probe Note If the replaceable tip loosens during sonication be sure to remove the tip for cleaning and inspect the threading on the tip and probe Call the manufacturer for assistance if the threading is chipped or damaged in anyway Replacement Tip Removal Replacement Tip Tightening 20 Rev 10 13 Microtip Removal Microtip Tightening Note When tightening a Microtip the tip must not be in contact with the work surface Always have the tip extending off of the table or work surface to minimize stress to the tip System Cleaning Instructions The generator and converter may be cleaned using an acid free cleaning solution i e glass cleaner Probes should be cleaned using isopropyl alcohol Probes a
22. the vessel could cause the Microtip to fracture Proper care of the probe is essential for dependable operation The intense cavitation will after usage for period of time cause the tip to erode and the power output to decrease The smoother and shinier the tip the more power will be transmitted into the sample The vibrations may also cause the probe tip to loosen over time or the threaded connection to accumulate debris Note A loose probe will usually generate a loud piercing or squealing sound For that reason it is recommended that a preventative maintenance schedule be adopted to examine the unit at regular intervals The schedule should depend on frequency of use Weekly maintenance schedules are recommended for units used frequently or monthly for those used infrequently The tip must be examined for excessive wear and to ensure that the threaded connection is clean and attached properly to the convertor Use a cotton swab and alcohol i e ethanol isopropyl etc to clean the threaded mating surfaces When excessive wear corrosion pitting of the probe tip is detected the probe should be replaced with a new one WARNING Hand tightening horns onto the convertor is not sufficient properly tighten them with the appropriate Wrench Set See below the steps for attaching and detaching microtip probes Follow the steps below for attaching and detaching accessories 1 Disconnect probe from convertor Use the wrench set provided wi
23. then press the ENTER REVIEW key The screen will display Pulse on 2 5 sec Pulse off _ _ sec 3 Using the numeric keys set the OFF portion of the cycle then press the ENTER REVIEW key The screen will display Pulse on 01 sec Pulse off 01 sec 4 Press the ENTER REVIEW key The screen will display Time 0 30 00 Pulse 01 01 Ampl 40 On Cycle Off Cycle 17 Rev 10 13 REVIEW The REVIEW function provides a window on the process by displaying various operating parameters without process interruption Pressing the ENTER REVIEW key repeatedly during processing will consecutively display the following information a Selected amplitude e g Amplitude 40 b Selected processing time and elapsed processing time e g Set 5 30 25 Time 0 57 03 c Selected pulsing cycle and actual pulsing cycle e g Pulse 2 5 1 0 1 5 5 d Amount of power in watts and accumulated amount of energy in JOULES delivered to the probe Note The amount of energy displayed will be only for one cycle Initiating a new cycle will reset the display to zero e g 20 watts 0000000 Joules e Elapsed time since processing was initiated e g Elapsed time 1 27 33 1 8 Rev 10 13 8 Maintenance It is recommended to periodically inspect the unit both visually and physically to insure optimum and safe performance This inspection should be scheduled as a routine maintenance procedure done with the ultrasonic processor power OFF and with the un
24. tion and violent collapse of microscopic bubbles The collapse of thousands of cavitation bubbles releases tremendous energy in the cavitation field The erosion and shock effect of the collapse of the cavitation bubble is the primary mechanism of fluid processing The probe tip diameter dictates the amount of sample that can be effectively processed Smaller tip diameters Microtip probes deliver high intensity sonication but the energy is focused within a small concentrated area Larger tip diameters can process larger volumes but offer lower intensity The choices of a generator and horns probes are matched to the volume viscosity and other parameters of the particular application Horns are available for both direct and indirect sonication The Accessories section has more information on this subject Please consult with a product specialist for assistance with selecting a probe for your application Relationship of Amplitude and Wattage Sonication power is measured in watts Amplitude is a measurement of the excursion of the tip of the probe probe is also known as a horn Some ultrasonic processors have a wattage display During operation the wattage displayed is the energy required to drive the radiating face of a probe at that specific amplitude setting against a specific load at that particular moment For example the unit experiences a higher load when processing viscous samples then when compared to aqueous samples The spe
25. tirety Necessary instruction and guidance are provided to help ensure the successful operation of this device Your new ultrasonic liquid processor has been designed built and tested to assure maximum operator safety However no design can completely protect against improper use that may lead to bodily injury and or property damage For total safety and equipment protection read the instruction manual carefully before attempting to operate this equipment Observe the following WARNINGS EM High voltage is present in the generator power supply converter and high frequency cable There are no user serviceable parts inside any of these devices Do NOT attempt to remove the generator cover or converter case Mi Do NOT touch any open cable connections on the unit while the power is turned ON Mm DoNOT operate generator with converter disconnected from high voltage cable High voltage is present in the cable and may pose a shock hazard m Do NOT attempt to disconnect the converter high voltage cable while the unit is running m The generator must be properly grounded with a 3 prong plug Test electrical outlet for proper grounding before plugging in unit MB Install the ultrasonic processor in an area free from excessive dust dirt explosive or corrosive fumes and protected from extremes in temperature and humidity Do not place the Generator within a Fume Hood Hearing protection is highly recommended It is recommended that a sound abatin
26. uids are problematic These liquids penetrate the probe replaceable tip interface and force the particulates into the threaded section isolating the tip from the probe When processing low surface tension liquids ALWAYS use a solid probe Set up 1 2 3 4 Connect the power cord into the receptacle on the rear of the ultrasonic processor Make sure the unit is switched off Plug the electrical line cord into the electrical outlet If the optional foot switch is used insert the plug into the jack located on the rear panel For best results it is critical to use the appropriate size and type of accessory to process your sample If you are not sure that you have the proper horn for your sample volume please refer to the product brochure for this system available at www sonicator com or call the manufacturer for assistance Horns Probes must be properly tightened Depending on the accessories purchased often the horn and the flat tip are attached to the converter at the factory Check the tightness of the horn and flat tip by using the wrench set Please refer to images in the Maintenance section of this manual A loose horn or tip may cause damage to the generator circuitry or parts of the converter and horn A loose horn may also show a fluctuation in wattage readings Always use the wrenches supplied with the unit If you will be using a Microtip or extender remove the flat tip on the end of the replaceable tip probe then attached th
27. ve an application that requires greater than 10 minutes of continuous processing there is potential for the probe converter assembly to increase in temperature The general rule is that if the converter is warm to the touch it should be cooled Besides cooling the sample another effective way to cool the converter is by using compressed air Each converter has 2 threaded ports for air cooling 5psi of dry clean air is required One port is attached to the air source and the other port remains open as a vent Black fittings shown below are available at no charge upon request E Standard Converter Feel for holes under the Attach fittings Attach air hose to one fitting label and use a razor to expose both holes Proper setup for air cooling the converter 24 Rev 10 13 Frequently Asked Questions FAQ Probe size vs Sample volume Selecting the proper size probe is a critical factor when sonicating a sample The sample volume to be processed must correlate with the tip diameter Each probe has a recommended sample volume range This range may overlap with other probes For example the 1 2 probe can process approximately 20 250ml Depending on the type of sample you may be able to process a little less than 20ml or more than 250ml Depending on the vessel size and shape the 4 probe may have difficulty fitting inside a 20ml volume and a 4 microtip may be a better option Many factors must be considered when selecting the
28. wnward As a sample is processed the liquid is pushed down and away in all directions If the vessel is too wide it will not mix effectively and some sample will remain untreated at the periphery Twice the volume in a narrow vessel takes a shorter time to process than the same volume in a wider vessel The probe should never touch the sides or bottom of the vessel How to prevent foaming small sample issue Foaming is a problem that often occurs with samples volumes below 1ml The cause of foaming is generally 2 issues too high of an amplitude setting or the tip is not immersed deep enough Some samples foam very easily and a Cup Horn should be considered Cup horns will not create foam 25 Rev 10 13 Tip depth The depth of the probe inside the sample vessel is another important issue If the probe is too close to the surface of the liquid it can create foam If the probe is too deep it may sonicate against the bottom of the vessel and not effectively processing the sample The sample must flow freely below the tip in order to be mixed effectively Without effective mixing you cannot ensure the entire sample volume will pass below the tip and become processed The probe should be immersed at least 1 5 times the tip diameter Before processing actual samples it is recommended to test the probe in a vessel filled with water to observe the ultrasonic energy and the flow pattern of the liquid During this test you can adjust the probe s depth until
29. you see adequate mixing and movement of the water Booster Horn A booster is a device that increases the amplitude intensity of a 1 or probe For example a 1 liter sample can be processed twice as fast with a 1 probe and booster when compared to the 1 probe used alone Smaller diameter probes already offer high intensity and will crack if used with a booster The booster may be beneficial when processing difficult samples with volumes above 500ml Power vs intensity Power is the measure of the electrical energy that is being delivered to the convertor It is measured in watts and displayed on the sonicator s screen At the convertor the electrical energy is transformed into mechanical energy It does this by exciting the piezoelectric crystals causing them to move in the longitudinal direction within the convertor This change from electrical into mechanical energy causes a motion that travels through the horn probe causing the tip to move up and down The distance of one movement up and down is called its amplitude The amplitude is adjustable Each probe has a maximum amplitude value For example with a diameter probe at setting 100 the probe will achieve an amplitude of approximately 120um At setting 50 the amplitude is approximately 60um Note this is approximate and not perfectly linear Qsonica measures the amplitude of each probe at 100 and these values are published in the brochure Amplitude and intensity
30. zes any person to assume for it any other obligations or liability in connection with the sale of its products The manufacturer hereby disclaims any warranty of either merchantability or fitness for a particular purpose No person or company is authorized to change modify or amend the terms of this warranty in any manner or fashion whatsoever Under no circumstances shall the manufacturer be liable to the purchaser or any other person for any incidental or consequential damages or loss of goodwill production or profit resulting from any malfunction or failure of its product This warranty does not apply to equipment that has been subject to unauthorized repair misuse abuse negligence or accident Equipment which shows evidence of having been used in violation of operating instructions or which has had the serial number altered or removed will be ineligible for service under this warranty All probes are manufactured to exacting specifications and are tuned to vibrate at a specific frequency Using an out of tune probe will cause damage to the equipment and may result in warranty nullification The manufacturer assumes no responsibility for probes fabricated by another party or for consequential damages resulting from their usage The aforementioned provisions do not extend the original warranty period of any product that has either been repaired or replaced by the manufacturer 4 Rev 10 13 2 Warnings Please read the manual in its en
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