Genetix QFill2 Manual
Contents
1. sess 2 P PINCH val Veens ette 4 6 9 18 19 Plate holder eesssesse m 13 14 Power outlet ste ete E SERE ERREUR 4 POW EISWIUCIN ie tiet e cnr rte erepto 10 Pressurisation cycle 11 Purge button sessssssssssseseenee eene 10 12 Purge tank sssssseeee 4 6 7 10 12 19 Purging the System sss 10 12 R REMOVE anaran c 4 19 Reset DUTON oce eva aber Reate even 11 Riean en e a AE 10 12 13 18 S Wl E 1 3 SALLY ca sc 3 4 Set Defaults ssessssssssssseese eee eren 14 Set Positions emere 14 Spare parts ecce etr ra Pertinet enr ente tits 20 Spill ag PH 4 7 T Troubleshooting sss 14 18 V Alc VOLUME PDC 6 11 12 13 14 W Warning labels ssssssseseeenne 3 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment EQUIPMENT DEMOS HUNDR2. A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com 7 information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED yop aed Contact us 888 88 SOURCE sales artisantg com www artisantg com User Manual QFill2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Copyright 2000 by Genetix Ltd All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted in any form by any means electronic mechanical by photocopying recording or otherwise without the prior written permission of Genetix Ltd Information furnished by Genetix Ltd is believed to be accurate and reliable howev
3. advance the wells will be filled and the plate will then return to its start position To abort the operation at any stage press the STOP key Dispensing will pause and the display will prompt you to press STOP to end or any other key to continue From time to time the carrier may pause and the message waiting for vessel to pressurise will be displayed This is normal Once the vessel has re pressurised the plate will advance and continue to fill Cleaning after use The QFill2 should be cleaned after each use refer to Cleaning Machine in the General Maintenance section page 20 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com pea EN TEN Menu Options To access the menu options press the MENU key There are 4 available options CHANGE VOLUMES This allows you to edit the 16 pre set volumes Use the 4 w keys to move to the memory position you wish to change To edit a certain volume press the ENTER key and then adjust the 4 digit value using the a keys The editor works in a similar way to a combination lock Each digit may be rolled up or down by one unit The ENTER key advances to the next digit Once you have modified the volume press ENTER Press menu to return to the menu screen and menu again to return to the opening screen To confirm the volume change press the VOL key scroll to the volume you have just altered and press ENTER The QFilD
4. may result in blocked tubes on the manifold Lastly switch the unit off and remove the silicone tubing from the pinch valve The bottle and cap silicone tubing manifolds and purge tank are autoclavable Artisan Technology Group Qualit Instrumentation Guaranteed H 989 99 ee Health R Safetv 4 To clean the manifold more thoroughly use the special screw supplied to pull off the manifold plugs at either end of the large tube They seal the tube with an o ring and may be simply pressed back into place once cleaning is complete Service and Maintenance We would strongly recommend that maintenance be carried out regularly and by a Genetix approved service engineer If you move the system repack it in the packaging material and boxes If the QFill2 needs to be returned to Genetix under warranty the machine must be returned in its original box to protect it during transit Please ensure that the Decontamination Certificate located in Appendix A is completed and that reasons for return are clearly stated The Product Return and Decontamination Certificate parts and II must be returned with the machine Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artibr dtbo amp Safety e 5 Initial Operation System Features The Genetix QFill2 consists of the following Base machine of approximately 30 centimetres square in size Pump running at approximately 3 5 psi e Pinch
5. plate retaining clamp opens sufficiently to allow plates to move onto the base plate from the stacker Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Connector Plate Base Plate Carriage Figure 6 Side View of QFill2 Pillars Side Plate Connector Plate n QStacker QFill2 Clamp activator bolt Pin Carriage Extension Figure 7 Top view of QStacker and QFill2 prior to connection Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Approx 3 mm gap Figure 8 Alignment of extension to carriage base plate Operating instructions Switch on both the QStacker and the QFill2 Press the RESET key on the QFill2 Set the required volume to be dispensed Load the WellPlates to be filled into a cassette and fit the cassette into the left hand position of the stacker Note Plates must be loaded with lids on Alternatively plates can be loaded into the cassette after it has been fitted to the machine The cassette will hold a maximum of 40 Genetix WellPlates For ease of application it is better to load all WellPlates in the same direction Fit an empty cassette into the right hand position of the stacker Ensure that the cassette engages with the clamps Press the START key on the QStacker and the machine will take plates from the left hand cassette to the QFill2 dispense liquid into the plat
6. pocket forming at the bottom of the well Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com m a d a E General Maintenance Note Always switch the machine off and remove the plug from the electricity supply before attempting any maintenance task There is very little in the way of routine maintenance that The Genetix QFilD requires The components have all been designed with very long mean time between failures and it is not anticipated that regular replacement of mechanical or electrical parts will be required for many years Cleaning the Machine After operation flush the system through with clean water and drain the dispensing bottle of any remaining liquid Replace the cap on to the bottle and purge the system with air only This is to remove any residual liquid from the tubing and the manifold Failure to carry out this operation may result in blocked tubes on the manifold Lastly switch the unit off and remove the silicone tubing from the pinch valve The bottle and cap silicone tubing manifolds and purge tank are autoclavable To clean the manifold more thoroughly use the special screw supplied to pull off the manifold plugs at either end of the large tube They seal the tube with an o ring and may be simply pressed back into place once cleaning is complete Cleaning QFill2 Components It is important that after a filling session has been compl
7. specifically for the QFill2 whose Serial Number appears in the box above If you find it necessary to modify or change these settings please contact Genetix Ltd for instructions Please Note changing these settings without supervision by Genetix personnel could affect your warranty Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com dA oen mme irt ud re Cc aa B Balls aor e em utter atria 19 Bottle 4 6 7 8 9 10 11 13 18 19 C Change Volumes servisine 14 Cleaning 2o cete ates 4 5 6 12 13 19 D DimenstOns cse tege neret ereesesite tease saat sitar setts 2 Dispensing Volume sese 11 12 E e i E 14 Electrical safety cccccsscesseceseeessesseeseseeereteseesetenen 4 Electrical supply ede tete res 2 Ethanol iat rer EE o ERR URS 19 F Filling the dispensing Bottle eee 9 G GENETIK nigasi vare dente 1 2 4 5 6 7 14 19 I Isolator switch sssseeene m 3 L LED DiSpl y uoce noeh teet ehe ria Rs 11 Liquid SPINA Se aiii ae ete gets 4 7 M Maintenance ccccccccccceesseesesssetseeeesesenseneees 5 13 19 Manifold 4 5 6 7 8 9 12 13 18 19 Manual dI 14 Me dl8 tisesacis ev co tre che eee rete careo rinsa 9 13 18 MENU Usseeetoe eere ire one ee n IRE ie eas pul Er AN PRINT QUEE EVE 14 Microtitte plates oceano eden ees 6 o Operating environment
8. 6 etre oe ER eerte eoe br Ele ipe atm FI EN rre RN edid 25 Index 27 Contents e ii Introduction Genetix QFill2 The QFill2 is an automated QPlate 384 well plate dispenser It is the ideal choice for filling the Genetix range of 384 or 96 WellPlates With this equipment you eliminate the laborious task of manual well filling The QFilD is ideal for microbial and cell culture applications or for aliquoting solutions in multi well plate assays such as plasmid mini preps or clean up procedures The QFill2 can fill 96 384 and 96 deep wells in less than 20 seconds Before using the QFill2 it is very important that you carefully read this manual and take notice of any safety instructions The QFill2 set up procedure must be adhered to This procedure ensures that the few adjustable parts on the QFill2 are correctly situated in their datum positions Failure to comply with this procedure can damage the Genetix QFill2 Note The Genetix QFill2 is strictly for research use only and is not intended or recommended for the diagnosis of disease in humans or animals Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisanigfeduction e 1 Technical Specifications QFill2 Manufactured by Genetix Ltd UK Dimensions Total assembled Size 30cm x 30cm x 20cm Weight 5Kg Operating Environment Temperature 10 C to 35 C Humidity 20 to 80 non condensing Electrical Suppl
9. EDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com 7 information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED yop aed Contact us 888 88 SOURCE sales artisantg com www artisantg com
10. always displays the last volume used SET POSITIONS Warning changing these settings may affect your Warranty This option allows the pre determined plate and well co ordinates to be altered and is not required for normal filling operations The values are pre set by Genetix personnel and should only be changed by Genetix engineers for troubleshooting purposes MANUAL FILL Having selected the required volume this menu will allow you to step the plate manually one row at a time Scroll to manual fill and press ENTER Place a plate in the plate holder and having selected the desired volume Press the v key to dispense the liquid and the key to move to the next row Press STOP twice at any time to abort the fill SET DEFAULTS Warning changing these settings may affect your Warranty This option also refers to plate and well co ordinates which do not require adjusting for normal operation It should only be used by Genetix engineers for troubleshooting purposes Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Ed EN I 1 FAI a EFI FaiL 4l 0 44A Assembly QStacker instructions Note Steps 1 3 only apply if you have purchased the QStacker independently I 2 10 11 12 Screw the base plate onto the QFill2 carriage Figure 6 Remove the three screws from the left hand side of the base of the QFilD Align the holes in the connector
11. d into pinch valve 4 Will not pressurise e Check top of media bottle is secured e Tubing is not connected to rear of QFilD e o ring s perished e Tubing fatigued e Bottle washer perished WellPlate lids jam diring operation e Switch machine off push down lever then remove lids Switch machine on and RESET Stacker fails to stop at end of test e Check that small metal lever in cassette hole has returned to its up position Plate s jammed in stacker e Switch off machine Do not try to pull cassettes stacks out of machine Push down lifter and remove cassette Clear jammed plates replace cassette and Switch machine back on RESET QFill2 Plates s jammed in stacker on entry or exit e Check that stacker height is 2mm lower from QFill2 than QFill2 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com lem NEN ma dem 1 ACIS Tenuhinehaatina a 40 Pusher in stacker misses plates e Damaged well plates e Pusher bent I i i e Damaged cassette preventing plates from dropping down Stacker stops for no apparent reason e Press START button e Plates may be stuck in cassette e Flag jammed in UP position Plates not moving freely up and down in e Cassette feet may be bent Stacker cassette Media splashes out of wells when filling e Angle the manifold slightly to avoid an air
12. d back plate Figure 2 8 way Manifold hole in back plate on left hand side For accurate filling the manifold must be purged of air first See the section on purging page 12 Connecting the dispensing bottle X Important Please make sure that you follow these instructions correctly Connecting the tubes wrongly will render your warranty null and void e Place the dispensing bottle to the side of the QFill2 e Screw the dispensing bottle lid onto the bottle ensuring a close airtight fit e Identify the stainless steel pipes on the top of the bottle The long angled pipe is the liquid outlet the short straight pipe is the air pressure inlet Liquid Outlet pipe Pressure Inlet pip qe Rubber Washer Figure 3 Bottle cap showing Inlet and Outlet pipes e You need two silicone tubes which are supplied with the QFill2 e Connect the first silicone tube to the Whatman Non Return filter which in turn Is connected to the short air pump outlet pipe at the back of the QFill2 Artisan Technology Greup Air Pump Outlet Figure 4 Rear view of QFill2 e Connect the first silicone tube to the QFilH2 manifold the steel tube with either 8 or 16 nozzles and then to the long angled stainless steel pipe on the dispensing bottle e Insert the first silicone tube into the pinch valve This is done by depressing the black button of the pinch valve and sliding the silicone tube into the slot making sure that
13. e and return it to the right hand cassette Once all the plates in the left hand cassette have been filled the machine will stop The right hand cassette containing the filled plates can then be removed by squeezing the levers on each side of the base of the cassette and lifting it out of the machine If the STOP key on the QStacker is pressed during a run the machine will finish filling the current plate then stop The run can be restarted by pressing START on the QStacker If the STOP key on the QFill2 is pressed during a run the machine will pause until either STOP is pressed or any other key is pressed to continue Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Troubleshooting Common Problems Problem Possible Cause Solution Air bubbles blow through the dispensing e Check that silicone tubing is connected to bottle liquid the correct tubes on the cap Leakage from manifold e Check that tube is fitted in pinch valve correctly e Check for leak s in silicone tube Low manifold nozzle pressure e Tighten bottle cap e Check that silicone tube is not blocked Not all tubes dispensing e Tube in manifold may be blocked br nera ws Pr ne ee ee Manifold not dispensing e Tubing has been left in pinch valve after a previous run and now has deformed I M Fluid continually flows from manifold e Make sure silicone tube is fully inserte
14. er no responsibility is assumed by Genetix Ltd for its use nor for any infringements of patents or other rights of third parties which may result from its use No license is granted by implication or otherwise under any patent rights of Genetix Ltd Revised February 13 2001 Genetix Ltd Queensway Stem Lane New Milton Hampshire BH25 5NN Tel 44 0 1425 624600 Fax 44 0 1425 624700 Email sales genetix co uk Web http www genetix co uk Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Contents Introduction 1 Genetix QFill2 eessssseseeeeeeeenne nn nennen hne hene eet inten en eene nennen nene l Technical Specifications 2 e dT PA 2 Health amp Safety 3 NOC eE sesh cosets dees cena scaecedidedtcosdtavnssd deascuseeSevbonpesidaicviencasnestegtveccnen serene seus Seedbteas at nentecncdtages 3 Safety Features a 3 Electrical safety ssssssssssseeeeee eene nenne entente Henne nennen eene nennen nnne 4 Drive Safety iiiisssssssssssssseeseee nenne netten then hene nne tnter tneent en neret enne 4 Cleaning Machine eiecit terere ttt nnt nnne tenete tenente tienne nennen nennen nnn 4 Service and MaintenanCe ssssessssseseeeseeeee nennen eene enne nena hee e ene nennen et tnrrrt eerte een ener 5 Initial Operation 6 YIUBICC IT RS 6 Pre Power Up Checklist essen eene nennen nnne 7 Power Up Procedures eee ne
15. eted the medium handling elements of the QFill2 are thoroughly cleaned with 70 ethanol manifold tubing dispensing bottle and plate carrier Failure to attend to this will lead to blocking of the manifold needles resulting in erratic and inaccurate dispensing of medium If this occurs the components are best cleaned in an ultrasonic bath filled with ethanol and the manifold needles cleaned with fine stainless steel wire Note If the manifold is dismantled check the condition of the o rings because they may perish after cleaning Artisan Technology Group Quality Instrumentation Guarantee LI Aes l a PEIPER an Recommended Spare Parts Spare Parts List for QFill2 8 Way Manifold 16 Way Manifold 500ml Bottle amp Cap Silicone Tubing per metre Purge Tank 1 Litre Bottle amp Cap 2 Litre Bottle amp Cap Deep 8 Way Manifold Deep 16 Way Manifold Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com pv Appendix B Calibration Information Q FILL 2 SERIAL No 6 IC VALUE Settings Adjustment DEFAULT CALIBRATION litre 1 litre 17 M Coarse 0016 I6 6 384 5 18 TOM Fine 0200 Acto A6S 19 NE Solenoid 01808 amp e4 S04 20 Coarse 0011 lt H 21 96 plates Fine 0200 26e A2Y10 22 L Solenoid ops 6H 64 The table shows a record of the calibration settings
16. ging the system The system must be purged of air for accurate filling From the following screen GENETIX READY Volume 0050 Plate type 96 press the PURGE key to see the following screen Press lt purge gt or stop to end At this point press and hold the PURGE key to run liquid through the tubing and manifold and expel any air in the system This function may also be used to flush water through the system for cleaning purposes When finished press the STOP key and empty the purge tank Artisan Te gy G ali entati arantee 388 88 R NWMLartisantg cam I leor Mannal COFill Diele Miaa FAL Preparation Fit the Silicone Mat e The silicone mat included in the packaging has moulded pegs on its underside e Place the mat so that it aligns with the edges of the moving Plate Holder e Make sure that the pegs are pushed firmly into the holes of the Plate Holder Set up the dispensing bottle and the manifold e Three quarter fill the dispensing bottle with the required media for the run e Fit the correct manifold for the type of plate you are using 8 way for 96 well plates 16 way for 384 well plates e Purge the system see page 12 Automatic Filling Place the first plate in the plate carrier making sure it is properly seated on the silicone mat e Ensure that you have keyed in the required volume e Press the START key and the carrier will automatically
17. hey are not perished e Fasten the bottle retaining clip onto the side of the unit using a flat bladed screwdriver to tighten as necessary Place the dispensing bottle inside this clip e Inspect the bottle for evidence of cracks scratches or any other damage e Fit the purge tank onto the front of the QFilD Always make sure that it 1s properly seated down The purge tank is designed to catch any spillage from the dispensing nozzles e n the event of a liquid spillage into the base cavity of the QFill2 the power supply should be disconnected at the mains before any attempt is made to clean up the spillage e Connect the power cable for the QFilI2 to your power source Fitting the manifold The QFill2 uses a stainless steel manifold with either 8 or 16 nozzles to dispense liquid evenly into wells 8 way manifolds are used for 96 WellPlates and 16 way manifolds are used for 384 WellPlates Standard manifolds are designed for Genetix plates but custom types are available for deeper well plates To fit the manifold insert the tapered end of the manifold back plate see Figure 2 on page 8 into the black slot in the centre of the QFill2 unit The machine will automatically detect whether an 8 or 16 way manifold is fitted by the hole in the back plate An 8 way manifold has the hole on the left see Figure 2 and the hole is on the right hand side of the back plate of a 16 way manifold bperation e 7 Tapered end of manifol
18. it is fully home e Connect the second silicone tube to the air pressure inlet pipe on the dispensing bottle lid This tube will pressurise the bottle so it must be airtight Important Double check that the tubing runs through the centre of the pinch valve Failure to do so may result in a spillage and will render your warranty null and void Figure 5 Filling the dispensing bottle If you have just connected the bottle according to the instructions above unscrew the bottle now but leave all the tubes connected The easiest way to do this is to hold the top still while rotating the glass bottle Fill up the dispensing bottle with the required media never more than three quarters full It is advisable that the first time you set up the QFill2 it is done with water Power Up Procedures e Switch on the machine The power switch is on the back panel e Press RESET on the keypad to pressurise QFill2 e Allow the machine to run for approximately thirty seconds in order to build up pressure in the dispensing bottle e Press the PURGE key The option to PURGE will be displayed Purging the system allows any air in the tubing to be displaced Press the PURGE key and hold it down until all the air has been removed Press the STOP key to return to the previous screen e Empty the purge tank and replace it on the machine Running a Filling Routine Initialising the machine The QFill2 features an eas
19. n n eene nen eerte 10 Running a Filling Routine 11 Initialising the machine sssssssseeeeeeneen eene enne nennen 11 Setting the Dispensing volume nete eene nnnm 12 Purging the System eene eterne te teetnns tnn tne nentes ton ah nta ran tate an atus ase ta sensus ta aetate tenent 12 Preparation netten ntt net nenne then eene tentent teenetetetenteentete ener tenennenens 13 Automatic Filling oie ertet tetra ere e ee HL E EV SS Eae E penne E Peer a cei eats 13 Cleaning after SE MEM 13 Menu Options cenres en R A A Eai Aia Eara 14 QStacker 15 Assembly insttUCtlOris nct er necp m avete es re eL de oap aeri e RR ede de 15 Operating iNStrUCtONS RN 17 Troubleshooting 18 Common Problems ode ote emp Rte rU EN edet ae cubre e te trea eres es pe 18 General Maintenance 20 p 20 Cleaning the Macliine oie rer i iecit cen tes as Th ul istos ie eee 20 Cleaning QFill2 Components aeree ttr naeia irase 20 Recommended Spare Parts 21 Spare Parts List for QEII2 actore IX et ute HE MERI Here Ee steve di A 21 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Contents i Appendix A 22 Product Return and Decontamination Certificate sssesssssssesnemee nennen 22 Appendix B 24 Calibration Information sese eren nnnm nnn ares n tisse t nsa nente trennen nn 24 Appendix C 25 OFN Packing E18
20. plate with the holes in the base of the QFill2 and replace the screws so that the connector plate is attached to the QFill2 with the pillars pointing up Figure 6 Place the QStacker next to the QFill2 so that the slots on the side plate fit over the pillars on the connector plate Figure 7 Push the QFill2 carriage across to the left and adjust the position of the QStacker so that the extension fits into the recess of the carriage Tighten the front pillar on the connector plate The height adjuster screw is located on the side plate Set this so that the extension is slightly below the base plate Place a WellPlate on the base plate and ensure that it can slide smoothly over the extension Connect the power output lead from the QStacker to the power socket on the OFill2 Connect the serial cable on the QStacker to the serial port on the QFill2 Connect the mains power to the QStacker Switch on the QFilD then the QStacker Press the RESET key on the QFill2 to make it home The END position for the QFill2 carriage should be preset by Genetix so that it aligns with the manifold The START position is preset by Genetix so that the gap between the base plate and the QStacker extension is about 3 mm Figure 8 As the carriage moves to the left to pick up a WellPlate the pin on the base plate strikes the clamp activator bolt on the QStacker Figure 7 causing the plate retaining clamp to open Adjust the clamp activator bolt so that the
21. trumentation Guaranteed 888 88 SOURCE www artianiitR OR Safety e 3 Electrical safety The QFill2 must be connected to a properly earthed power outlet to protect users from the risk of electric shock The main chassis of the machine is earthed together with all associated electrical components Do not remove any of the fixed covers as there are no user serviceable parts inside All internal work should be referred to Genetix approved service personnel The QFill2 has a Warranty seal and is marked with tamper evident sealant if any of the covers are removed the guarantee is rendered null and void In the event of a liquid spillage into the base cavity of the QFilD the power supply should be disconnected at the mains before any attempt is made to clean up the spillage Drive Safety The QFill2 incorporates a moving plate tray Keep objects and fingers clear from this mechanism when the robot is in operation The Pre Power Up Checklist on page 7 should be followed before every routine in order to prevent serious damage to the QFill2 or any of its constituent parts Cleaning Machine The machine should be cleaned after use After operation flush the system through with clean water and drain the dispensing bottle of any remaining liquid Replace the cap on to the bottle and purge the system with air only This is to remove any residual liquid from the tubing and the manifold Failure to carry out this operation
22. valve which controls the working volume of medium in user defined quantities dispensed by the machine e Motorised table which holds a 384 or 96 well microtitre plate e Purge tank Manifolds 16 way suitable for 384 well microtitre plates and 8 way manifold suitable for 96 well microtitre plates e Pressurised dispensing bottle with silicone tubing e Manual e Bottle holder ring e Manifold cleaning screw e Mains Lead Pinch Valve e Silicone Mat Inlet tube from short straight pipe to air pump Keypad Outlet tube from angled pipe through Pinch Valve to manifold Silicone Mat Manifold Plate Holder Figure 1 Purge Tank Capacities The QFilD is normally configured and calibrated at the factory to work with 0 5 litre and 1 0 litre bottles If re calibration is necessary refer to Appendix B Note please ensure that the serial number recorded in the manual matches the serial number of the machine because each machine is calibrated individually to ensure optimum performance Artisan Technotogy Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisawigiCelBeration 6 Pre Power Up Checklist e Check that the master switch on the back panel of the QFill2 is in the OFF position and plug the power cable into the back of the unit e Check that the tubing is not fatigued e Make sure that the sealing washer on the bottle cap is not perished e Check the o rings on each manifold plug to make sure that t
23. y Voltage is fixed at either 110vac or 240vac depending upon geographical market Make sure your QFill2 is set for the correct supply voltage before attempting to connect to a power supply European Voltage AC210 250V 45 65 Hz single phase Max Current 250mA Power 50W Connections IEC inlet USA Japan Voltage AC 90 120V 45 65 Hz single phase Max Current 500mA Power 50W Connections IEC inlet MW articanta com Tachnical Sneacifications e 2 Health amp Safety Note Before using the QFill2 please make sure that you have read the manual and comply with the following safety directions Prior to using the QFill2 please make sure that you have been through the QFill2 Pre Power Up checklist to ensure that the QFill2 is set up correctly Safety Features Isolator Switch Located at the back of the machine this turns the power on or off The LCD panel indicates whether or not the power is on Start Stop Located on the keypad pressing this during operation will interrupt a fill routine Warning Labels The machine is marked with hazard warning labels All warning labels must be observed Non Return Filter Valve The Whatman Filter valve is fitted on the rear port of the machine and will prevent fluid entering the machine in the event that the tubes are fitted the wrong way round If the Whatman Filter valve becomes wet or damaged it must be replaced zz rtisaa technology Group Quality Ins
24. y to use menu interface on its integral LCD display Once the unit has been set up and the dispensing bottle filled it is a simple process to fill large numbers of plates with a precisely measured volume of liquid After powering up the QFilD the LCD display will show the following message GENETIX Ready to RESET Software Vere LON After the QFill2 is switched on press the RESET key This will initialise the drive system and start the pressurisation cycle The bottle must be connected and airtight at this point You should briefly see this display GENETIX Wait for vessel To pressurise If you do not see the following display within about 30 seconds check that all the tubes are connected and the bottle top is screwed on tightly GENETIX READY Volume 0050 Plate The dispensing volume and plate type 96 or 384 will be displayed The volume displayed is always the last volume used Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com X Xa MB qus FsI 110 Setting the Dispensing volume e Press the VOL key to view the volume options 0050 0060 0070 0080 mH G NO S e Scroll through the volume options using the w keys e When the asterisk is next to the desired volume press the ENTER key The screen will now display the chosen volume e To return to the previous screen at any time press the CLEAR key Pur
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