Microplate Reader
Contents
1. screen 9 1 1 Serial NO 2 Hospital hospital name 3 Date and time 4 Printer 2100C support 5 series printers Canon BJC 250 series HP DJ 340 400 series HP LJ SL 6L series Epson LQ series Epson Stylus series please see such windows UN Serial No 123456788 Hospital Cold harbor hospital B Date pooo p2 pe Time y fit pe Printer Epson stylus series y 3 Contrast Canon BJC250 series HP DJ 340 400 series lt p Sound HP LJ5L 6L series Epson LQ series screen 9 2 You can choose one of printers NOTE If printer setting is wrong maybe printer works error 5 Contrast adjust press button to increase contrast press button to decrease contrast New value will be saved and used 6 Sound switching Turn on or off the speaker 7 Touch panel calibration You can calibration touch panel Press touch panel button come to calibration windows 40 2100C Operator s Manual Screen 9 3 Press the cross center by pen wait one second the cross moves to next place automatically You should continue press cross with pen again The same operation repeats 5 times system will display next windows NOTE A If the cross dosen t move after press it for many times the touch panel may gose bad please contact your dealer Screen 9 4 Please click anywhere in the panel system will save new setting and come back system setting windows 41 2100C Operator s Manual 102. 44 2100C Operator s Manual 12 POWER OFF Press Power off button in main menu display such windows 2100C X 2 Power off Are you sure Press yes button instrument will be turned off Press no button exit to main menu PAN NOTE If don t turn off instrument normally results and parameters edit in this time will not be save System will display such windows Please waiting ta Saving data j IK Ba screen 12 2 ATTENTION Cautions Please keep power when turn instrument off The instrument may fail to operate normally if the power is interrupted When data saved such windows will display Now vou can tum the power switch off safely NOTE N Turn 2100c off for at least 30 seconds and turn it on again 45 2100C Operator s Manual 13 INSTRUMENT SERVICE 13 1 Maintenance 2100C is designed to be a maintenance free instrument To insure optimum trouble free performance the instrument should be kept dry and operated in an area free from excessive dust 13 2 Cleaning The Instrument e Keep working environment clean e Use a slightly damp soft cloth to remove dirt or spills For decontamination 70 isopropanol 1s recommended e Use soft cloth to remove dirt or spills in LCD ATTENTION Cautions Use other chemicals or abrasive cleaners are not recommended 13 3 Changing Part Of Instrument 13 3 1 Changing the fuses 1 Disco
3. Full name Calculate Screen 5 1 Program List You can pre program 100 tests in 2100C 5 1 Mode setting 2100C support 9 calculation modes 5 1 1 ABS mode 2100C only display and print out absorbance of samples 5 1 2 Cut Off mode Cut Off formula Cov X x NC Y x PC Fac In this cut off mode where NC is the mean of the negative controls PC is the mean of the positive controls Cov is the cutoff absorbance X Y Fac are coefficients that have any positive and negative numerical value including O and 1 e g sample OD NC12 1 is positive that X 2 1 Y 0 Fac 0 In regular cutoff mode 2100C award that sample is positive if sample OD Cov gt 1 5 1 3 Calculation mode 1 Single standard mode A single calibrator material of known concentration is used so that concentrations of unknown samples may be calculated according to Beer s Law Abs Conc Figure 5 1 Single Standard Mode 2100C Operator s Manual 2 point to point mode you can set 2 8 standards the resulting calibrator curve is a series of lines connecting the calibrator points which may be entered in ascending or descending order of absorbance Abs Abs Conc Conc Figure 5 2 Point to Point Mode 3 ABS You can set 2 8 standards The highest absorbance standard will be assigned a value of 100 and each sample and standard is reported as a percent of the absorbance of the highest calibrator The resulting calibrator curve is a series of lines connecting t
4. INFORMATION MANAGEMENT Press information button in main menu come to the following windows Operator de Department _ Sender Delete Cancel Screen 10 1 2100C has operator database and department database Youcan add or delete operators department in those database 2100C can store 100 records in those two databases Select operator or department For example operator database 1 add record press add button input operator name in windows input operator name Screen 10 2 Press OK button operator s name add to list If this operator s name already exists system will display windows 2100 jx Aeperator jack exists Screen 10 3 If database reach high range system will report operator s records is over 2 Deltree record Select one operator in operators listing press Delete button 42 2100C Operator s Manual Cy Del jack are you sure Screen 10 4 Press Yes button to deltree that record press No button to cancel 43 2100C Operator s Manual 11 SYSTEM LOG Press event log button in main menu you can view history log _ Date Time __ Type Clear Cancel Screen 11 1 Event log contain date time type and event status 2100C can record max 2500 events Last event arrange firstly You can press Clear button to deltree all event records Press Cancel button to come back main menu
5. 10 Storing Test Results Press cancel button in Abs windows The instrument will automatically store the test results in history database 30 2100C Operator s Manual 7 REPORT 7 1 Report form Press report in main menu system display such windows Canon BIC 250 series Report by patient OProgram Date Today OAl Cancel Select report form Screen 7 1 e Report form report by patient list or program list e Date range today and all of days NOTE e Before you print the report please check whether the cable between the printer and instrument has been correctly connected and the printer has power on e Always make sure that there is sufficient paper in the printer Never use the printer without paper 7 2 Report By Patient list Select patient press Ok button display following windows EN CE a Screen 7 2 1 Select one patient 2 Mark multi patients You can mark all patients by pointing first line in the title bar 7 2 1 Edit patient data Select one patient press Edit button patient s information windows pop up 31 2100C Operator s Manual Input patient information A Date 2003 06 09 SampleNO 001 e Name Age Sex Male 3 Female 8 Unknow Dept xl Operator Bed ID Sender y er Ele Screen 7 3 Input the information Press Ok button to save information press cancel button to exit without sav
6. Filter wheel error Open the cover of the instrument check sensor 50 Plate does not come back original place Plate don t move Open COM port error Printer don t work The printing is dim or incomplete The printing is jam Other printer error 2100C Operator s Manual The plate may be does not place in right place Check it Driver Motor error open cover checks driver motor work or not Maybe use serial mouse Turn 2100C off Disconnect the mouse from instrument and turn on it again Printer power works error Check power is ok Check ON OFF button in printer Check printer type in system setting right or not Firstly turn 2100C on then turn printer on Check printer cable connect 2100C right or not Change ink box clean printer head See printer user manual See printer user manual See printer user manual ATTENTION Cautions Trouble shootings above are for trained operators reference If unsolvable error is found during the operation process or appears again and again untrained users should contact your supplier for further help S1
7. Please disconnect serial mouse from 2100C and connect serial cable to 2600C Waiting connection Send Cancel screen A 1d 3 Open RI 2600C power switch begin sending data 4 Press Cancel button after fimshed 5 Turn RE2600 off disconnect serial cable from RT 2600c NOTE If system happens error press Cancel button to stop mission If this error exists Please contact your dealer 38 2100C Operator s Manual 8 3 Communication to PC 2100C can connect to pc send data to pc With software you can store patient lists see curves print reports and collect QC data For more information regarding pc software contact your dealer 1 Turn 2100C and pc connect serial cable to 2100C and plug another end in pe COM 1 ATTENTION Cautions 2100C and PC don t open when connecting serial port otherwise hardware will be wrong 2 Press Pc button in communication windows come to next windows Waiting Waiting connection Send Cancel Screen 8 14 3 Run pc software select data send menu receive data 4 Sending data finished press Cancel button to come back main menu 39 2100C Operator s Manual 9 SYSTEM SETTING Press system setting button in main menu Serial No 123456758 Hospital Cold harbor hospital E Date pooo j p2 i pe Time po apa ope 5 Printer a T k Contrast Ee Touch panel PE Sound S0n off Cancel
8. absorption of light is proportional to the amount of light absorbing substance in the well Lamber Beer s law A axbxc Where A absorbency a absorptivity of the substance b Optical length and c Liquid concentration The advantage of the vertical light path measurement are 1 inaccurate pipetting of nonabsorbing liquids does not affect the measured absorbance values 2 Evaporation of nonabsorbing liquids during the reaction does not affect the measured absorbance values and a certain degree of inhomogeneity in the solution for example as a result of layering in turbidity measurements does not affect the results 2 3 2 Optical system The optical system comprises the following components Figure 2 3 lamp membrane board filter wheel fibre reference path microplate seconday lens O WANN A FW WN optical diode A D transfer board pa CO 2100C Operator s Manual heat absorbing board and lens Figure 2 3 Optical system 2 3 Technical Specifications Weight Overall dimensions Power Fuses Work Environment Store Environment Lamp Standard Wavelength Abs range Measurement range Accuracy Precision Linearity Reading speed Warm up time CPU Store content Interface Display 10kg 450mm L x330mm W x 140mm H AC110V AC250V 50 60Hz 250V 3 15A temperature 10 C 40 C Humidity 20 85 20 50 OSRAM64607 8V SOW 405 450 492 630nm substitute filters from 340 700nm available on sp
9. e SOW ATTENTION Cautions e Ac power must be grounded at the main socket Interuption of the protective conductor inside or outside the instrument or discoonection of the protective conductor terminal may make the instrument dangerous e The circuit used should be substantially free of large transients such as large pumps large centrifuges etc e If found smog strange sound in instrument please turn instrument off inmediately and contact your dealer 2100C Operator s Manual Connect the supplied power cable to the rear of the instrument as shown Plug the other end of the power cable into an AC outlet which has a protective conductor also called as earth or ground 1 4 External Printer Connection With both the instrument and the external printer off connect the parallel cable to the rear of the instrument Plug the other end of the parallel cable into the printer Then plug power cable into printer Install printer paper if needed 1 5 Transporting for service Repack the instrument according to instructions enclosed in the package Use the original package Indicate the fault Inform about the use of hazardous materials 2100C Operator s Manual 2 FUNCTIONAL DESCRIPTION 2 1 Introduction 1 2100C is a microprocessor controlled general purpose photometer system designed to read and calculate the result of assays including contagion tumorous mark hemopathy dyshormonism which are read in microtiter plate 2 T
10. proceed according to the instructors and do not touch any parts of the instrument other than those specified Verify the front covers closed while the instrument in operation Avoid touching the mechanism such as the plate holder mechanism inside the instrument while the instrument is operating This may cause operation stop or damage the instrument 2100C Operator s Manual 1 INSTALLATION 1 1 Unpacking Unpack 2100C carefully and check for any damage that may have been caused during transportation check the components e 2100C instrument e Operators manual e Packing list e Accessories panel pen power cable print cable RS 232 serial cable pstn line lamp fuses Keep the original package for future transportation NOTE e Report any damage or missing to you local representative e Retain the original packing material for future use in the event that the instrument is placed in storage shipped to another location or returned for service 1 2 Environmental Requirements Locate 2100C to avoid exposure to excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations Leave sufficient clearance 10 cm at both sides of the unit for adequate air circulation ATTENTION Cautions Instrument be operated within an ambient temperature range of 10c 40c humidity of 20 85 1 3 Electrical Setup Power requirements e ACIIOV AC250V e 50 60Hz
11. system display windows 21000 es 2 Are you sure delete ABS Screen 5 7 Press Yes button to delete program Press NO button to cancel 5 5 Standard Standard test results and curve will be stored Operator can review and print the results Select one program in program listing Press standard button system display standard conc and 23 2100C Operator s Manual abs Calibrator cone af Abs A 1 0 000 0 000 E 1 000 0 500 3 2 000 0 800 4 3 000 1 000 Program Demo Calculation mode Reg Screen 5 8 Press curve button system will display standard curve 1 100 A 0 550 0 000 M4 0 000 1 500 g l 3 000 Program Demo Calculation mode Reg screen 5 9 Press print button system will print standard curve NOTE Old standards will be r deleted if modifying program setting Wavelength calculate mode standard number standard conc etc 24 2100C Operator s Manual 6 RUN THE TESTS 6 1 Plate Parameters Setting Press test icon in main menu pop up such windows Select test parameter Plate direction 1 12 CAH Test mode continue gt Step mixer speed None mixer time S Screen 6 1 e Plate direction A H direction and 1 12 direction e Test mode You can select continue mode lt 5 seconds or step mode lt 15 seconds e Mixer setting Mixer speed you can se
12. 1 3 e Range of Negative and positive if absorbance of negative control and positive control is out of range 2100C calculates as min or max abs e Double of controls 1f select this controls must be pipetted into consecutive two wells the mean absorbance reading will be used to calculate a single concentration 3 Positive and negative setting windows 22 2100C Operator s Manual Qualitative Positive threshold value Senso Pasilivesad Negative setting Window Qualitative mode In this option you can select regular cov reverse cov none e Regular cov Samples with values higher than the positive cutoff are labeled as positive Sample with values lower than the negative cutoff are labeled as negative Any samples with values falling between the negative cutoff and the positive cutoff are labeled as equivocal e Reverse cov Samples with values lower than the positive cutoff are labeled as positive Sample with values higher than the negative cutoff are labeled as negative e None you can select none if you don t award negative or positive Normal range please input the values with reagent instruction Press Finished button come back program listing windows 5 3 Creating program Press new button to create a new program then input the parameters If the program name has existed system will warning program exists 5 4 Deleting program Select one program in program listing press delete button
13. TUES A Saarde andre tanneri A et nnee ded td daeeedint 46 1332 Ch neme he LANA tii etaoin snadra licita olis 46 1333 Chanson net verneem eneen eeen 47 13 34 Chaneme the IONE BOA Za dl anta 47 13 54 Chaneine the photocell merento kearni toi 47 13 5 0 Mancini the plats holder dio diei s 48 15 30 Chaneine thedrvno BOA iia 48 133 7 Chaneine the ECD transfer OA wanne eenden daneen 49 13 39 Ghanees the MDI siii oda 49 15 9 SCN maeTr trat d ti 50 ISA TrOUDIEShOOLMS os 50 2100C Operator s Manual How to use the menu General Welcome to be the user of 2100C This manual is a user s guide for 2100C microplate reader which includes system installation Operating procedures parameters setting and basic maintenance Reading the manual carefully before operation is strongly recommended For external printer please refer to related external printer manuals Instruments with different software versions will have some different functions this manual describes the system 2100C and its software version Symbols description Symbols in the manual m NOTE Notes contain additional information or tips when using the instrument ATTENTION Cautions Cautions should be followed carefully to ensure your instrument work correctly and to avoid unnecessary personal injury Symbols on the instrument 2100C Operator s Manual Precautions General Before you start installing and working with themicroplate reader you should rea
14. black boxes remove the four special screws which fix work area and the three screw which fix the head of fiber then lift up the work area carefully 2 Disconnect all the wires from the driving board remove the screws and replace the driving board with a new one then recover all of them ATTENTION Cautions e Protect the fiber e Protect from static electricity e Don t confuse the wires of the filter wheel motor and the wires of power supply wires e Don t leave out the insulated pad The driving board Figure 13 4 The driving board 48 2100C Operator s Manual 13 3 7 Changing the LCD transfer board 1 Open the upper cover of the instrument and take off the black box 2 Disconnect all the cables on the transfer board and loose the screws 3 Install a new transfer board LCD transfer board Figure 13 5 The driving board 13 3 8 Changing the fibre 1 Remove the only screw open the upper cover and remove the four screws then take off the two black cover Loose the three screws which fix the head of the fiber use a stick to push the head backward carefully use a special screwdriver to remove the four special screws which fix the work area finally remove the two screws which fix the frame of fiber 2 carefully Remove the two screws which fix the fiber take off the fiber and install the new fiber 3 Install the frame with the fiber located on fix the frame to the work area then install the fiber hea
15. cutive two wells the mean absorbance reading will be used to calculate a single concentration e Blank You can set range of the blank abs If blank absorbance out of range system will display blank abs is out of range You can press gt gt button come to next windows or press lt lt button come back this windows you can press cancel button exit program configuration windows 1 Standards mode setting windows Standard Number 4 Standard conc 1 0 000 Unit fuji i 2 1 000 _ Double standard 3 2 000 4 3 000 E i a Screen 5 3 Standard Window e Standard number you can select up to 8 e Concentration unit you can select it from listing e Double standards if select it standards must be pipetted into consecutive two wells the mean absorbance reading will be used to calculate a single concentration 21 2100C Operator s Manual e Standard conc double point standard conc Listing you can input standard concentration Input standard conc Screen 5 4 Input da Notice Standards may be entered in ascending order of absorbance Standard 1 is the lowest concentration 2 Cut Off mode setting windows Cutoff Expressions 100 xmNC 000 xmPC p 000 Range of Negative Range of positive Double of controls Screen 5 5 Cut Off Mode Setting Window e Cut Off formula parameter Cov X N Y P Fac Please see section 5
16. d and work area carefully Screws fix the fibre head Figure 13 6 The fibre crews fix the fibre frame Figure 13 7 49 13 3 9 Changing the fibre 2100C Operator s Manual 1 Disassemble the instrument disassemble the black boxes which cover the light disassemble the work area carefully disconnect all the wires which connect to the switch 2 Remove the fixed screws and screws in the bottom Install a new power supply and connect all wire 3 Pay more attention to the fiber loose the screws which fix the fiber first and then disassemble the work area 13 4 Troubleshooting Symptom 2100C does not Turn on Lamp does not light RAM check error Lamp is too high Lamp is too low Motor does not work Chopper wheel run faster Filter does not come back zero place he power supply Figure 13 6 The power supply Causes and Remedies Check power supply cord Check fuses Check power voltage Turn 2100C off for 30 seconds and turn it back on Check lamp Voltage Lamp is bad Change lamp Turn 2100C off for 30 seconds and turn it back on Firstly turn 2100C on and secondly turn printer on Cable connect error turn 2100C off open instrument cover and recomnect cable between two pcb Filters install in wrong place Lamp is bad and changes it Motor 1s bad Open the cover of the instrument check motor Chopper driver motor error Open the cover of the instrument check chopper driver motor
17. d the safety precautions and regulations shown in this chapter Operator Qualification Please note that the operation with Microplate reader should be carried out only by the doctor or clinical inspector who have undergone necessary training provided by the sales agent Service Technician Qualification TO install maintain and repair the instrument a service technician has to be trained on the instrument by the manufacturer or their representative A service technician is also expected to be familiar with the normal operation of the instrument as described in the User s manual and the special operations as described in the service manual Electrical To use microplate reader safely pay attention to the following items TO prevent the risk of electrical shock and or damage to the instrument Operator should not open the white cover of the instrument Only authorized personnel for example service technicians may open the instrument to perform maintenance or repair Touching the main board when the power is on may cause severe injury or death Any problem please ask for helps from your supplier Mechanical There is no risk presented by the mechanical parts of the instrument when the instrument is closed If the covers of the instruments are removed mechanical parts could cause personal injury or the instrument may be damaged if the following advice is not being considered DO NOT wear loose garments or jewellery that could catch
18. deeetenms 23 A A O O EE o alee cationic 23 OPS ONE A De EE 25 O Plate Parameters Setna edito E 29 602 Select Test Pro Cran ies sonen eenen ON 25 OSMAR Welt tai 26 OA e EC M aos 21 69 CIE AU les 28 OA IEN Sire ade ES 28 0 TROU pisito bibi dial bano 28 2100C Operator s Manual 6 9 Calculation Rol dass 29 6 9 A mannenteam 30 6 10 Storm TestiResullS annie eet ndaneded 30 REPORTE o ao 31 CNO Otro lts tl 31 RA REPON By Patent USL orn derhednok saken oen ordedrohn T a bededeohe nae 31 Teal Beit patient AAA mie a a 31 T De A en eben eea et By ee or MOC Viele ae e A ohne 32 Te ae NMA ORG A N 32 7 3 Pant By Prost aii ISEren AN 32 deal A A A A 33 132 WEIS POSTA eolica ao Aosta 33 A a AAA A A aclacigatedelen ate owns tease scaieat an delet es westia 33 Heo A o a eier eenn ten eneen 33 S EONMENCATI N nn tetes 35 A LL Ue ret 35 8 1 1 DiD meteen EE A Sela ate on uel anen 35 8 1 2 Data MEATS IMIS SIO eten tases O 36 8 1 3 Ee Xa COMMUNI Call ON nda 38 S 2 Communication to 20006 iuie a a iio ete 38 825 Commumedtonto PE A beke Meerten 39 DS SEEN SEDEINGS a A a 40 10 INFORMATION MANAGEMENT cocccoooocccnnnnnnnocnnnnnnnnnonnnnnnnonnnnnnnnnonnnnnnnnnnonnnnnnnnnnnnnas 42 SS MEA EC eeen nternet 44 12 ON A 5 EN EERE EEE 45 5 INSTRUMENT SERVICE inenen tie eeen ie ed 45 15 INSERUNMENDTSERMIGH sa hence eee E 46 SA NTL DANCE dl eo cana ede dune ace 46 t32 Cleaning Fhe AAA nternet 46 185 Chanemo Part OL IMS tu ment ii 46 AO eme the
19. e 7 2 2 Preview report Select a patient press preview button that patient s data display in list Program name Screen 7 4 7 2 3 Delete patient data Press delete button windows pop up o an li Press yes button all marked patient data will be delete Press no button exit without save 7 2 4 Print report Press print button all marked patient report will be print 7 3 Print By Program list Select program press ok button and pop up program list windows 32 2100C Operator s Manual ho Program Testdate screen 7 6 7 3 1 Preview report Select one program press preview button and pop up preview windows a LJ Well_ Sample Abs __ Result Screen 7 7 7 3 2 Delete program Press delete button and yes button all marked program and test data will be delete 7 3 3 Print report Press print button all marked program s standard curve and test data will be print 7 3 4 Gather print 2100C support gather print you can quickly print all negative or positive patient data in one day Press gather button come to next windows Input condition Screen 7 8 q 2100C Operator s Manual Input date select program and results from list press OK button all patients coincidence condition display in listing windows m eeen ee A Screen 7 9 Press print button all data will be print
20. ecial order 0 3 500A 0 2 000A 0 2 0A 1 0 or 0 007A 0 2 0A 0 5 or 0 005A 2 0 or 0 007A Continuous mode lt 5s step by step mode lt 15s 1 minute Embedded RISC cpu 500 test 1000 patient information 10000 test data RS 232C serial interface parallel printer interface telephone line modem interface 5 7 LCD display 320x240 discernibility 256 gray scale Input 2100C Operator s Manual Touch panel and pen external mouse special order 14 2100C Operator s Manual 3 OPERATION INSTRUCTIONS 3 1 Touch Panel amp Pen 2100C has a touch panel you can operator the instrument by pen ATTENTION Cautions Please use the pen to touch the panel NOTE You also can use a RS 232 mouse to operate the instrument 3 2 Screen 3 1 Digital Number Keyboard Input integer age float number abs value digital number phone code press OK button to saved or press cancel button to exit and no saved Edit press k button to del a character before cursor Press clear button to clear all characters 3 3 Character Soft Keyboard Esc 112 3 4 5 6 7 8 9 0 Tab ajwje r t yjufifo o t 1 Capjalsjd fjO hjijkjIj Shift z x c v b njm 4 cofat 4 te Screen 3 2 Character Soft Keyboard You can operator this keyboard like PC keyboard and move windows by dragging 2100C Operator s Manual Use your mouse to click here and drag away the soft key board Please remembe
21. ed 34 2100C Operator s Manual 8 COMMUNICATION Communication function is an option function for 2100C Plus Click communication icon in main menu The following windows will be displayed Communication Screen 8 1 8 1 Remote service 8 1 1 Dial up Press Telnet button come to dial up windows Screen 8 2 1 Connect to service center Input service center phone code press Dial up button 2 Connect to internet Screen 8 3 35 2100C Operator s Manual Operator input user name password and Internet IP address Press Dial up button Connect to 0 88888 Screen 8 4 System will come to telnet service windows if dial up succeeds Connect to 202 222 16 03 Connection successful Screen 8 5 8 1 2 Data transmission Connection successful will be display in this telnet service window Now you can press buttons to transmit data 1 Send data Press Send data button come to next windows Sending content Y History data p parameter Y Control data Ih parameter Y System log Screen 8 6 Select sending content press Ok button and send data send data Program database 30720 TT Total 888000 Sended time 00 00 06 Screen 8 7 36 2100C Operator s Manual Press Cancel button to stop sending data 2 Receive data Press Receive data button come to next windows Program param
22. eter Hardware parameter OK Cancel screen 8 8 Select receive content press Ok button receive data Receive data Program parameter 5120 jaa Total 62112 Sended time 00 00 02 Cancel Screen 8 9 Press Cancel button to stop receive data 3 System update Press update button 2100C will receive update data Receive update data System data 19456 MB Total 230440 Sended time 00 00 08 Screen 8 10 Press Cancel button to stop receive data When system received data ok update data to Flash ROM Now update system Don t touch anything gan Screen 8 11 37 2100C Operator s Manual ATTENTION Cautions The instrument may fail to operate normally if the power supply is interrupted during this time System will display windows after update successful wf xj System update ok p Please turn power off and start again Screen A 12 Now you can exit telnet come back to main menu turn instrument off 8 1 3 Exit communication Press Cancel button in telnet service windows press Cancel button in dial up windows and come back to main menu 8 2 Communication to 2600C 2100C can connect to 2600C update 2600C s software 1 Close 2600C power connect 2100C with 2600C by serial port cable 2 Press 2600C button in communication windows system display such windows NOTE If serial mouse connect to 2100C
23. he calibrator points which may be entered in ascending or descending order of absorbance 4 Linear regression mode You can set 2 8 standards This is a multi point standard mode that calculates a best fit linear equation based upon the standard points Equations Y kX b This is used when the absorbance and the concentration is linear Abs onc Figure 5 3 Linear Regression Mode 5 Index regression mode You can set 2 8 standards Equation Y ke This is used when the natural log of the absorbance is plotted against the concentration 6 Logarithm regression mode you can set 2 8 standards Equation Y kLnX b This is used when the natural log of the concentration is plotted against the absorbance 7 Exponent regression mode You can set 2 8 standards Equation Y kX This is used when the natural log of the absorbance is plotted against the natural log of the concentration PY Edit Program Setting Select existed program 1t will be marked press Edit button display the following windows 20 2100C Operator s Manual Program H bs4g Full name Reagent First wavelength 430 Second wavelength None d Calibaration mode absorbance Double sample Blank 0 050 Screen 5 2 Program Window e program program name don t input the name existed e reagent Input reagent name you can ignore it e wavelength e calibration mode e Double sample If select it samples must be pipetted into conse
24. he power supply wire and flat signal cables then remove the fixed screws 2 Install the front board to its location pay attention to the power wires and small flat cable which are through the bottom of front board 3 Connect the cables and wires fix the screws and pay attention to the detector which should not be lifted up Photocell Ti 13 3 The front board 13 3 4 Changing the photocell 1 Open the upper cover take off the two black covers Disassemble the front board then remove the two screws which fix the photocell as shown in figure 13 3 2 Use a soldering iron to take the photocell off from the front board Install a new photocell and fix the screws then install the front board 47 2100C Operator s Manual 13 3 5 Changing the plate holder 1 Open the upper cover remove the two black cover remove the fixed screws which fix the frame take off the frame and rails 2 Loose the adjusted screw which fix the belt you can see the adjusted screw when moving the holder out Overturn the plate holder and remove the two fixed screws then replace the plate holder with a new one 3 Screw the screws overturn the plate holder back screw the adjusted screw to adjust the belt to suitable position then install the rail and frame LN Het Adjusted screw which fix the belt The frame gt The rail Figure 13 4 The plate carrier 13 3 6 Changing the driving board 1 Open the upper cover take off the two big
25. in mechanisms DO NOT put your fingers hands into the pathway or any part while the microplate reader is in operation DO NOT attempt mechanical repair unless the instrument is not in operation or OFF Lamp The source lamp becomes extremely hot during operation never touch the lamp when it is on Look directly into the light path of the lamp may cause eyes damage too 2100C Operator s Manual If the lamp needs to be changed always switch off the lamp by switching off the instrument and then wait until the lamp has cooled down Chemical The operator is responsible for taking all necessary precautions against hazards associated with the use of clinical laboratory chemicals Specific recommendations for each reagent used with the microplate reader are normally found on the manufacturer s package inserts or the on the product information sheets for each chemical Wipe up any reagent spillage on the instrument immediately Biohazardous Materials As with all vitro diagnostic equipment patient samples and serum based quality control QC products that are assayed on this system as well as all waste from the waste container should be treated as the potentially biohazardous All materials and mechanical components associated with the sampling and waste system should be handled according to your facility s biohazard procedure Use the personal protective equipment recommended by your facility when handling any of these components Detailed
26. lect fast normal slow or none Mixer time you can select 1 60 seconds Press Ok button come to next windows Inmmonwr r Program Calculation Mew Select all Clear all Screen 6 2 6 2 Select Test Program 2100C support 12 programs in one plate first press New button the following windows display Select a program 3 Screen 6 3 25 2100C Operator s Manual select the program press OK button o NOTE If you test multi programs in one plate you must set the programs one i 5 by one STD standard CLR clear 6 3 Mark well You can set each well s function Select well s function firstly and click the well which you will mark 4 B i D E F G H Program ABS Calculation Absorbance New Select all Clear all screen 6 4 mark label sample 1 999 blank B negative controls NC positive controls PC standard S1 S8 Quality Control QC 6 2 1 Sample Click the well which will be tested then the well will be marked If you modify sample No please click that well again A windows display Input No 1 9989 Input sample No again If you input No already exist system display sample NO Already exist Input again 26 2100C Operator s Manual NOTE One patient is marked by only one sample NO In one day Negative controls NC Positive controls PC 6 2 2 Blank You can select blank well or no in different program One progra
27. m display 2 Are you sure clear all e Press yes button clear all well setting 6 6 Test Press start button system will test the plate e Mn Testing screen 6 7 You can press stop button to stop the test 6 7 Results The test results will be displayed l 2 3 4 5 6 A otho Oo 8i Bibo Eso Soo nn in a Screen 6 28 2100C Operator s Manual 1 6 columns abs results will be displayed press 7 12 gt gt button windows will display 7 12 columns abs results Sample 001 is sample number 0 040 is abs results NOTE j N if abs results gt 3 500A will display 3 500 and calculate by 3 500 if PB abs lt 0 000A display 0 000 and calculation by 0 000 you can modify the well result by click the well Change Abs 0 3 000 ci ox Jeanca mM m Input abs value and press ok button NOTE Standard or control abs change will affect all results in this program 6 8 Calculation Results Press result button in abs result windows display calibration results I nmo nomo gt Screen 6 9 You can see the values and negative positive mark display in every well If the values in some well lt 0 0 will be labeled to this well 29 2100C Operator s Manual 6 9 Printing NOTE OTA quantitative analysis OLA qualitative analysis Press Print button in testing windows 2100C will print all test results 6
28. m only set a blank well Old blank value will be saved automatically and be used 6 2 3 Negative controls You must set one negative control in one program other than negative control factor is 0 6 2 4 Positive controls You can see negative control setting Section 6 2 5 Standard This option is valid only when one program needs standards If this program has old standards values you can select set standards or not NOTE If one program set standards and test good new values of standards will cover old values 6 2 6 Clear If you want delete marked wells press clear button then point wells that what you want 6 4 Select All Use this button you can quickly mark the sample wells Press select all button display such windows Input start No amp well Start No 00 Start well la y input start number 1 999 and start well row number and line number press Ok button All wells in this plate will be marked samples Start No 1s that you input In this option all set program and wells will be deleted You should set standards and controls after select all 2d 2100C Operator s Manual i 2 10 11 12 OOOO 2 skh ozs wel re ac atone asta H oss 086 os7 ose 083 030 031 092 093 094 095 0396 Program ABS Calculation 4bsorbance Cancel ammo rT oo Sp olojo la lo Rus fs Un Us aJ Screen 6 5 6 5 Clear All Press Clear all button syste
29. mre En OPERATION MANUAL FOR UT 2100C Microplate Reader M R C LTD OFFICES HAGAVISH 3 HOLON 58394 P O B 1684 TEL AVIV 61016 ISRAEL TEL 972 3 5593105 972 3 5595252 FAX 972 3 5594529 2100C Operator s Manual Table of Contents HowWto Use te Mei ia on 4 Precautions a A SS 5 Me DEF DO EE TO eea E ippo opi sias ooo 9 Na acre heya cia eastrcee seca No i tr cele enero ate ese seca No ar ele ena a ek eee er eae 9 t2 Environmental RK CQUITEMEINS swennen eenderde etende 9 SAB TEC UIC Al SMUD adi id i n 9 A External Printer CON lOs 10 LS Eras PORUNG TOR Service idad 10 2 FUNG TIONAL DESCRIP TION eriin aen 11 DV MEOGUCIION Antenne neee eene eene 11 AA A AM A a a R E 11 PO AN A OENE ll EA A VIEW sca neta seach elas acca lotr ae E ese auaunsaedea 12 2 3 Measurement PrnciPlenin dida lili ar 12 Zal EnOtometio Measurement a 12 232 Optical System ndo 12 2S Toena Speci ICAU ONS rav 13 Se OPERA HON TNSTRUCIONS adas dais 5 a NE Bro Fe a et Vie ke e Oe II ona ene nen erent entree erent AS 3 2 Digital Number Keyboard nd AS IoC harder SOM KENDO A EE a ES E INSTRUMENT STARTUP surta a A E 17 AA POWERON niente heeele A ic 17 ZNA MACAU PA rn eye ieee me atee 18 O A O 19 De de MOE CUA sa 19 5 1 1 ABS MOE teca 19 5 1 2 CU ON MOE aii a a 19 SA3 alcuno mode zenne dede 19 gt Z Edit Progra A O E T T 20 Ov Be ALS D OEI aE eea Anbergen 23 JA DEELS 0 1 97 4214 0 DEPOR RCP CO E Geter aaeaeieuwice se aasaxea
30. nnect power cord from mains supply before replacing fuses 2 Open fuses box left in power change fuses close fuse box Fuse specification 250V 3 15A 3 Turn the instrument on ATTENTION Cautions Use the same fuse for replacing 13 3 2 Changing the lamp 1 Turn the instrument off open instrument cover by unscrewing the one screw on the front of the instrument 2 Remove four screws of the optical assembly cover Remove the cover 3 Lift up the lamp with the terminal socket Pull the terminal socket from the lamp contacts 4 Refit the terminal socket to the contacts of the new lamp OSRAM64607 8V SOW Place the new lamp in its place 5 Close the optical assembly cover Replace the screws 6 Close the instrument cover Turn 2100C on again Screw fixed the lamp Power supply wire Figure 13 1 The lamp 46 2100C Operator s Manual 13 3 3 Changing the filter 1 Open the upper cover take off the two black covers Remove the two special screws which fix the filter wheel motor 2 Loose the three screws which fix the filter wheel pull the motor out and take the filter wheel out 3 Replace the relative defective filter with a new one Install the filter wheel and the motor Special screws Screw fix the filter wheel Figure 13 2 The filter wheel ATTENTION Cautions Don t scrape the surface of filter 13 3 4 Changing the front board 1 Open the upper cover take off the two black covers Disconnect t
31. ouch panel let your operation easily 3 100 pre programmed tests 4 Multiple calculations e Absorbance mode ABS e Cut Off mode Single standard mode Point to point mode Absorbance Multi Point Mode Linear regression mode Exponent regression mode Logarithm regression mode e Power regression mode 5 visualization mark plate you can set blank control sample standard in any place and run mostly 12 different tests in one 96 microplate 6 Test time 5s plate and mix plate before test 7 Max save 500 program and 1000 patients data and 10000 sample records 8 generalization report support multi type printers 9 2100C can update software 2100C also can connect to 2600C and update 2600C s software 10 information manage function Department database operator database system log database 2 2 General Description 2 2 1 2100c front view Figure 2 1 Front View 1 power pilot lamp It light when opens the instrument 2100C Operator s Manual 2 touch panel display program 3 Plastic cover 4 Plate carrierer Microplate in plate carrier 2 2 2 2100c rear view awe Ve Le Figure 2 2 Rear View 1 Power switch 2 Power sokect 3 Fuses 4 PSTN connector 5 PRINTER interface 6 RS 232 interface 2 3 Measurement Principle 2 3 1 Photometic Measurements 2100C utilizes the concept of vertical photometry in which the light beam passes through the whole sample In vertical photometry the
32. r not to loose the mouse during dragging Figure 3 3 move the soft keyboard NOTE Click the area which ts just above the soft key board a little See the figure above 2100C Operator s Manual 4 INSTRUMENT STARTUP 4 1 Power on Switch the instrument on using power switch and wait 10 seconds you will see such display 2100C Microplate Reader Version 2 0e Copyright C 2000 All Rights Reserved Screen 4 1 System Startup instrument will self check and initialization data will be load i EMS mnmbelizetor Loading system Screen4 2 System Initialization Loading system System self check Initialization data Initialization of system Wait for the light of lamp stable this will take about 1 minute NOTE A The result will vibrate if the light is not stable Self check of light path The filter wheel will turn around and the plate holder will move in and out for one time Error report will be display if the instrument has not pass the self check NOTE A If the instrument has been connected to printer plase turn on the instrument before you turn on the printer If the order is wrong tt may cause self check error or the printer can t print normally 2100C Operator s Manual 4 2 Main menu After self check system display main menu Screen 4 3 Main Menu 18 2100C Operator s Manual 5 PROGRAM Press program icon program list will be display Mo Program
33. recommendations Samples Treat all samples as potentially biohaazardous and infectious If any sample is spit on the instrument utilize the correct personal protective equipment PPE gloves lab coat etc wipe it up immediately and clean the contaminated with a disinfectant Biohazardous parts Avoid direct contact with the microplate Treat these areas as potentially biohazardous and or infectious Reagents Avoid direct body contact with reagents Direct body contact may result in irritation or damage to your skin Refer to the manufacturer s reagent kit box and package inserts or product information sheets for specific instructions Avoid direct body contact with cleaning solution Direct body contact amy result in skin irritation or damage Refer to the manufacturer s kit box and package inserts or product information sheets for specific instructions 2100C Operator s Manual ATTENTION Cautions Requirement for Samples and Reagents calibrators and controls Our Microplate Reader is a Reagent open system But before the testing the following items should be noted first Generally all the reagent and samples have to be stored and prepared as per the manufactures instructions We do recommend that these reagent should from the manufactures with valid product quality certificate and production permission legalized by local government or from the valid distributors appointed by MRC LTD in your country Before your
34. test please check the following Check the expiration date of the material Check if the reagent is stored properly as the requirement e g cooled or frozen storage before using Check for proper programming in parameter setting menu according to the specified reagent instructions Check for volume required for each material Check for proper and calibrated pipette to prepare the solution Additional Precautions Flammables Avoid using dangerous flammable material around the instrument Accuracy Precision of the Measured Results For proper use of the instrument measure control samples and monitor the instrument during the operation An incorrectly measured result may lead to an error in diagnosis thereby posing a danger to the patient Treat all reagents according to the manufacturer s recommendations Refer to the reagent kit box and package inserts or product information sheets for specific instructions Make sure that the sample reagent mixture does not contain any blood clots dust or other insoluble contaminants If insoluble contaminants are contained in the sample correct measuring values may not be obtained Application The instrument is designed for clinical immunity test analysis using water soluble samples and reagents Please note that other types of analysis may not be applicable to the instrument 2100C Operator s Manual Operation and Maintenance During operation and maintenance of the instrument
Download Pdf Manuals
Related Search