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1. opringerLink User Manual SpringerLink is one of the world s leading interactive databases for Scientific Technical and Medical STM books and journals Springer has continuously advanced SpringerLink to meet the research needs of the scientific community Our interactive database builds on the strengths that have made SpringerLink a world class exploratory tool with features and benefits that can significantly enhance the research process This User Manual provides a complete and easy to follow guide to SpringerLink users springerlink com e ENTER TO START gt gt Springer Link SpringerLink User Manual Version 1 00 Issue Date June 2004 SpringerLink SpringerLink joins electronic and printed literature from opringer Verlag a preeminent scientific publisher with a reputation for excellence spanning more than 150 years It also offers the work of a growing roster of publishers including Urban and Vogel Steinkopff and Birkh user Started in 1996 SpringerLink was one of the first online information services dedicated to scientific research Today it is one of the world s busiest with millions of downloads every month We have continuously advanced SpringerLink to meet the research needs of the scientific community Our interactive database builds on the strengths that have made SpringerLink a world class exploratory tool with features and benefits that can significantly enhance the r
2. Articles E 0 SenncH ABOUT EROWSE FAVORITES gt Home Browse Results Criteria All Publishers 6 Springer Verlag France First page Address 1 rue Paul Cezanne 75008 Paris France Previous page 7 Springer Verlag Heidelberg pe sti Address Tiergartenstrae 17 D 69 8 Springer Verlag HongKong Ltd Address Unit 1702 Tower Enterprise Square 9 Sheung Yue Bay HK Kowloon Hong Kong 9 Springer Verlag Italia Srl Address via Decembrio 28 I 20137 Milano Italy Springer Verlag London Ltd Address Sweetapple House Catteshall Road Godalming Surrey GU 3DJ England 6to 10 of 15 sidelberg Germany e Click the publisher link and go to more the list of publications for this publisher For assistance inside the Americas springerlink springer ny com For assistance outside th Springer Verlag Heidelberg Tiergartenstr 17 D 53121 Heidelberg Germany PrivacN Copyright Information amp Harris i Beie Festns i Publisher Springer Verlag Heidelberg Navigation 151 to 200 af 127 Tiergastenstr ae 17 D 50121 Hoeidelher Germany hel A 351 bmenrnstonal Jounal of infomation Secwrtty Pr sious publisker p Hari publisher A 9152 International Journal of Legal Medicina iv 453 TERN Fest page 153 h derssmonal Jor or Dirk Lbranes Pr b 58 Intimeastmnal Jounal en Document Arahe and Becaognitiun Hs page 4
3. Browse Publications Online Libraries Criteria Filter Viewable Publications Only Return Journals Hook Series Results Display 50 Hits Per Page Copyright 2004 SpringerLink Page 21 aCK tO 1ADIE OF UONtENtS All Rights Reserved User Manual Version 1 00 Browse Results go 1 Abdominal Imaging 1 to 50 of 526 Publisher Springer Verlag Mew York go 2 Accreditation and Quality Assurance l Hext page Publisher Springer Verlag Heidelberg Last page g 3 Acta Diabetoloqica Publisher Springer Verlag Italia Srl Jump To go 4 Acta ethologica ABCDEFGHIJKLM Publisher Springer verlag Heidelberg NHOPORS TOY Vy 7 Sb 5 Acta Informatica Publisher Springer Verlag Heidelberg ga 6 Acta Mallrernmalica Sinica Publisher Springer Verlag Heidelberg go F Acta Mathematicae Applicatae Sinica English Series Publisher Springer Verlag Heidelberg x 8 Acta Mechanica Publisher Springer Verlag Wien go 9 Acta Mechanica Solida Sinica Publisher Springer Verlag Heidelberg sg 10 Acta Neurochirurgica Publisher Springer Verlag vier so 11 Acta Neuropathologica Publisher Springer Verlag Heidelberg go 12 Advances in Biochemical Engineering Biotechnology Publisher Springer Verlag Heidelberg du 13 Advances in Pokaner Science Publisher Springer Verlag Heidelberg go 14 Advances in Solid State Physics Publisher Springer Verlag Heidelberg Note the total number of
4. Take Cd Cobana Alert i Table Of Contents Alert Publications 1ta 105 of 455 Ez Abdorrinal Imapng Meat pope fg Arcreditafion and Quality Assurance Lect ag 7 Arta Diabetologica ume To EE Arta atholagika 7 Arta Infzrmztira EC 7 Atta Malhematica Sines e Aria Malhematitan amp pplestae Sinica Erglish Farias Check your favorite CE owe dn enable publications for Table Cd Contents Aer DO Arta Merkaniea 7 Atta Mechanica Solda Sinica ET Select or deselect pybbestigers from ihe listo dtes at me Jer publications Arla Foeurachiningica C Arta Pe urapst alngies Click h re to browsa iata of puetiradinng omanzed inl sykler reds E Adances i Bochemical Ergineenngidireechnalogr E Advances in Fome Science anmas bi EX RE To canos edarkan witho Advances in Eclid State Ftwsics agetyi wur changes click C equatioras Mathemaicaa Cancer C Asshole Plastic Surgery Jm E Or click subjects in Table of Contents Alert to get the full list of subjects Online Libraries Users can then select their favorites subjects and titles b Horne i Talis Cd Contes Alert I Table Of Contents Alert Subjects Tesine indi 3 Huw do enable publications Por Tabla OG Contents Alani Click here to get the list of books and journals Y Selecta subject fram the list al Len fa b l Io rea the es wihi il p hal hiemaric
5. th a Pewne 005 AT HELP 4357 Copyright amp 2004 SpringerLink Page 61 aCK to ladle Of VONtents All Rights Reserved User Manual Version 1 00 Users click the CrossRef icon SpringerLink will forward you to the original article References Click the CrossRef icon which will forward you to i LER Schmid A Hauer E Mikal E 0005 I Bir Chem 273 1165 1082 Fu 2 Suzuki M Hayakawa T Shaw J Rakix M Harayama amp 1997 J Bactanal 173 1620 1606 Chee miferi the home page where the Ex mhankin J mile 2 Fee Ets 193 Bieckemiety 33112787 1 2724 Chart Iu Pubid Snila THEM Fot hi sus article is hosted zm Eramn Miceobial 1730 317 Kener A 1927 Anges Chem Irt Ed Engl 31 774 775 Fanke 5 1 Germ er Huber Chi Pilhak Bl Wtobboks hie 15684 Appl Ermron Microbiol 65 0924 2532 Leahy JG Cohel BR 1990 Microtel Rev 24 205 315 este rued Harapama 5 Kishia H Kasai Y Shutsudo K 19899 J Mal Microbiol Biotechnol 1 53 70 Ortiz de hlaniel ana PR fed 1995 Cytechrame PHD structure mechanism awd bioctiermislry 2nd edn Plenum Her Tork ACS ELIA TN HHIHZXHOGLITY HEZH INE SG P Eaedbaek Purchase Hydraxylstion of Camphor by Reduced Duy Cytochrome PF 45 ram Mechanistic Implications of EPR and ERDOR Studies of Catalytic Intermediates Im Hatiee and iatankt Enzymes Roman Baspdoa Thomas PL Marie vicbera Kalma
6. E mail sv taipei msa hinet net Springer Verlag Taipei Office 4Fl No 335 Sec 1 Jianguao S Rd Daan Chiu Taipei Taiwan 106 R O C Tel 886 2 2703 3556 Fax 886 2 2703 1644 SPRINGERLINK HELPDESK E mail springerlink springer de Copyright 2004 SpringerLink Page 68 ACK tO lable OF GONTENIS All Rights Reserved Springer Link Springer Birkh user ip ak SpringerWienNewYork Physica Verlag f STEIN KOPFF GUN CONTACT INFORMATION CHINA HONG KONG SOUTHEAST ASIA SOUTH KOREA Grace Lau E mail gracel springer com hk Maurice Kwong E mail mauricek springer com hk Edwin Chu E mail edwinc springer com hk Springer Verlag Hong Kong Limited Unit 1702 Tower 1 Enterprise Square 9 Sheung Yuet Road Kowloon Bay Hong Kong Tel 852 2723 9698 Fax 852 2724 2366 E mail springer springer com hk TAIWAN Leo Lo E mail sv taipei msa hinet net Springer Verlag Taipei Office 4F1 No 335 Sec 1 Jianguo S Rd Daan Chiu Taipei Taiwan 106 R O C Tel 886 2 2703 3556 Fax 886 2 2703 1644 SPRINGERLINK HELPDESK E mail springerlink springer de
7. urman Perescy Dakare Terne anc conde d Cop id oria oi Click the Properties button in the Table of Contents Alert to get to the following screen Users can choose how to receive the alert from SpringerLink when logged in or by email SPRINGER rna i mA ES re PE S 8 C f ink SEARCH FOR RETURN BROWSE Table Of Contents Alert Properties Properties When receiving new issue alerts please Notify me when I log in Notify me by email Also include issue contents as 9 HTML O Text For assistance inside the Americas springerlink springer ny com For assistance outside the Americas springerlink springer de Springer Verlag Heidelberg Tiergartenstr 17 D 69121 Heidelberg Germany Privacy Disclaimer Terms and Conditions Copyright Information Copyright amp 2004 SpringerLink Page 55 ACK to lable of vontents All Rights Reserved User Manual Version 1 00 4 2 2 Keyword Alert A more specific alerting service can be found in SpringerLink called Keyword Alert It allows users to define a number of keywords for new contents alerts Whenever there is any new publications posted that contain the keywords the user will receive an alert Users can edit their choices as their research needs change Keyword Alert You can edit delete or view the Tea existing keyword 1 einen HEEF For assistanre maida ne Americas soip con For ssiebanre du
8. vr TET Copyright amp 2004 SpringerLink Page 62 ack to ladle OT VONtents All Rights Reserved User Manual Version 1 00 5 2 Bread Crumb With the Bread Crumb navigation SpringerLink lets users quickly retrace their steps without relying on the back button on the web browser Users can click on the thread to return to previous steps in one click t Hei Tate Cd Conte dest Tole Of Conbenes Sel Subieocts Table Of Contents Alert Subjects Bread Crumb navigation Chemical Sciences Accreditation and Quality Assurance Ad are ez In BEacbamircal Enang cieu Einki E 4n Pee GF How d enable peblications Adearetes in Polymer Science Tar Table Of Conternis alert numina Acids O Dse grt ie das ler putricabons Anabtical and Beaanaiytics Cheemistre fram fre ixl artries organized inks subjact artas ofthe let Anplad Malhemaics avd Opimization i Applad Microbiology ard Blotachnalogy Click here fo brente lets of publestiors not nigarezed ink C amp ppiad Phisics A Baterialg Science amp Freocegcing sublect areas anpied Piria Be Bal CI npied Pirties E Lasers seg Opies S Archives for History of Exact Sciences cornplels list of Subjects areas dick Cantnue Arches af Emdiranmarntl Conlariratien and Todcolopy i a Baipracasa ad Blosyslems Enmane eig Contre Bulein of Ere eronmenisi Contamination and T eininge Ifyou hawt finished selecting Chromalogrsehia publea
9. 17 CEAN Haidekerg Cernsey Prisc Disclsrer Terne andi Conditions d Coprgfi huormalion Copyright amp 2004 SpringerLink Page 51 ack to ladle Of VONtents All Rights Reserved User Manual Version 1 00 4 2 1 Table of Contents Alert Users can set up Table of Contents Alerts for those publications most relevant to their research needs Users can choose their alerts either by publication name or subject area Select by Publications Table Of Contents Alert MERECE To configure Tabla Of Conmaniz alee stu mas broweza a complete Isl cf pudlicataon dins ard gelari pour rhialzasg indsidgslk You mag algo birate Erbe of okie aon organi init aubj amas Dirionale ag mar ousiomine a deler maou alerts Publications select by Ercarae let of puzlicstans and sisteriyvaur choices indria ll Subjects Show only my current seacor LU 5ihpents Ercawsa Gate af publicalions perganized ima subject armas m j Remove all Dirse AT Diable Table Of Contents Aled ror all publeatiore CET l settings Properlies Custambe the deker af yaur abara CARS Update Erai Adiye Chara fu gmail zidrass you weld lika yaur Pro perties abita sert mm Fried zrimasng vill nnt ha Click Publications in Table of Contents Alert to get the full list of publications Users can then select their favorites here Copyright 2004 SpringerLink Page 52 aCK to ladle OT VONtents All Rights Reserved User Manual Version 1 00 amp Hore
10. 5 5 peeig Backto Table of Contents C Page 18 ack to Table of Contents opyright amp 2004 SpringerLink All Rights Reserved User Manual Version 1 00 To best display the hit list please do not select too large a number in the display results The default is 50 items per page The following sample screen shows 50 hits per page gt SEARCH FOR RETURN ABOUT BROWSE gt Home Browse Results o 1 Abdominal Imaging 1to 50 of 496 Publisher Springer Verlag New York 69 2 Accreditation and Quality Assurance Next page Last page Publisher Springer Verlag Heidelberg Acta Diabetologica Publisher Springer Verlag Italia Srl Jump To g 4 Acta ethologica ABCDEFGHIJKLM NOP RSTUVW Z Publisher Springer Verlag Heidelberg Acta Informatica Publisher Springer Verlag Heidelberg Acta Mathematica Sinica Publisher Springer Verlag Heidelberg Acta Mathematicae Applicatae Sinica English Series Publisher Springer Verlag Heidelberg Acta Mechanica FisilslindBsnses iacu iinel Archives of Gynecology and Obstetrics Publisher Springer Verlag Heidelberg Archives of Microbiology Publisher Springer Verlag Heidelberg Archives of Orthopaedic and Trauma Surgery Publisher Springer Verlag Heidelberg Archives of Toxicology Publisher Springer Verlag Heidelberg Archives of Virology Publisher Springer Verlag Wien Archives o
11. Ccatabtic donari Publication Joala Biotygiral ino ganit C hemestr inhi pelo Gha MY Serergen 7 ve aral 11a TD of 173 Number of Haxt pae hits Live pipe Filter Selected kem search Wb Hess 1 Search wilnin Nas Ngee Filter option Fre Copper Further search within the results The number of hits is displayed at the top right hand corner of the Results page and the search results are listed by title plus an excerpt of the article is shown Users can see the contents of an article by clicking on the article link Users can make further searches within the search results to refine the search Simply type in the additional keyword s on the Search Within Results box on the Search Results screen to do another search within search results By checking the boxes of the selected articles and clicking on the Filter Selected Items button will allow users to refine their results EG 2004 Sn Pado Backto Table of Contents Page 40 aCK to IaDie or vontents Copyright amp 2004 SpringerLink All Rights Reserved User Manual Click the link to get the contents Search Results Criteria enzymce AND copper AHO copper Fact of emerat sustitutinn in Gapped amine oxidasn fron earn seedlings Publication Joma of Biological lnorgandc C herniak Authors Padoglia Rosada Medda Jens I Faderzen otal Publisher Springer Vardag Heidelberg Fecenos volume 4 Humber 5 Rehanna 3 09 Escenpt hzbaciTheresctnn
12. Dh elie ean Humber 5 hee 594 Abd Humber 41373 497 Minter 34235 371 Taio Of Contents Alert Edt Copyright 2004 SpringerLink Page 39 ACK tO lable OF LONLENTS All Rights Reserved User Manual Version 1 00 opringerLink displays the search results as follows Criteria enzyme a p Bie p eneral eultestitulimn in copper amine gyan fort Held sembra Publicatior Journal of Biological Inoegarde Chenncslre Authors Padslia Resana Medda Jana Z Fadarsgen otat Publisher S rryervarag Haldebang Recency volume 4 Humber 5 Rekuancc 19 5 E rgrptt Abstract The reaction wil substrates and carborsl reaganis af pale nil Cyu srrine ceidaze and its mode meme ig Ci Tul depleted Cu hal reccersttuled Cu tulk resonstisled Co substined NI substiieted and Zr zubstluted has been studied Lipan removal of ony ona of fa c Cu l ns Z2 Ca and nhe Bacterial ger oxrasets The cytochrome c perosatasp borti Poaceae Tucan Publkatior Joursal of Biological Inorganic Chemie Authors Crnsbna G Trneten Pedro Tavares Gell F Goodhew e ar Publisher Sprger telag Peidehberg Hecera Values 3 Fumbers 1 3 Relevancy 17 5 Excenp amp bstacl The pradurban rc olosfeome mercedes DOP Imm Fraudcereana Pot duke ATCC 116071 wzz ppimized by adjusting ie Oneness hon Of Te gemit medium ans eee arthia cultura The groe was inobsipd and zhaarierged Biorchan itat 3 taectken replacement of thet catakeie zinc af ie farta airy i
13. Electronic ISSN use http y Sspringerlink comopenurl asp genre articleseissn 1 432 1 327 volume 9 amp i2sue 24spage 119 To link with the Digital Object Identifier DOG use http fav springerlink com openurl asp7genre article amp id dol 10 10071500775 003 0519 7 Note there are 3 different Links found in 3 different levels ie in each Journal each Issue and each Article Copyright amp 2004 SpringerLink Page 31 ack to ladle Of VONtents All Rights Reserved User Manual Version 1 00 About This Journal Description of this publication Description The Journal of Biological Inorganic Chemistry covers advanc systems involving ane or more metal ions setin a biological metalloproteins and metal nucleic acid complexes in order to urimersrermarmemem function at the molecular level Synthetic analogues mimicking function Per Bd spectroscopy of naturally occurring biological molecules are also of interest ta the jaurnal Manuscripts describing high quality and original research concerned with metal ians or other inorganic species and having biological relevance are invited for submission to this journal The journal publishes original articles mini reviews and commentaries on debated issues ISI Impact Factor A Publication of the Society of Biological Inorganic Chemistry information Impact factor Biochemistry amp molecular biology 2002 Rank 61 af 2661 Impact factor 3 Chemistry i
14. a sample of a downloaded Excel file J ureal Frint 1534 cka Hechanica Acta Heurochirurglca Online 1554 ipen p Ahdoninal Peagi i it 00 Accreditation and Quality amp cearance ficta Disbetologica BIAI Acta ethologica 8F3 F7ThF Acta Informatica dubii 5940 Bete Hathenatica Sinica 1437 8514 gadi 5yzu 19 8937 Acta Hechanica Solids Sinica gudi 4z68 Bcta Heurnpathalagica Bb 4537 Advances im Biocherlcal Enginsering Bieterhnology mMS 3175 Advances imn Folv r 5riemcr Ed Microsoft Excel All Journal URLs xls Dc E GR 4B o Springer Verlag New York Springer Verlag Heidelberg Springer Verlag Italia Srl Springer Verlag Heidelberg Springer Verlag Heidelberg Springer Verlag Heidelberg Springer Verlag Heidelberg Springer Verlag Wien Springer Verlag Heidelberg Springer Verlag Wien Springer Verlag Heidelberg amp r f 2 flg 557 Publisher Abdominal Imaging Accreditation and Quality Assurance Acta Diabetologica Acta ethologica Acta Informatica Acta Mathematica Sinica Acta Mathematicae Applicatae Sinica English Series Acta Mechanica Acta Mechanica Solida Sinica Acta Neurochirurgica Acta Neuropathologica 1432 0509 BYKD 177E T S 1837 9586 Th39 74517 Acta Hathenatirar Applicatar Sisica English Series Version 1 00 http Sem springerlink coasepenir a 1422 0517 http sy fone springerl i http fwaw zpringrrlink com epenurl a httpsfsrven springer Link cun open
15. e a 1 n d ihermmsstable branching enzyme from an exizemel ihezmagphidic haz al spe les Bhodocshermus Clans Public mio Applied Hizrabielag arid Eratezhnelagg Authors Hari L Shinohara Hihiko Ihara Wazan bu Abn ef ar Publisher Eprinoer Verlag He delzarng Re ncy volume 57 Murmbars Sf Reievancy 220 Excerpt A branching me EIC 2 4 1125 gene was 50 bled from an g ramaly tregmozhile bariem Ascites obits Tha rated senten anendes a heels OS ami alt siti a predated enaterul amp r ds of z Ho The deduced seno Click the link to get the contents e D fubeuks in Enza Techrinioog Li Cont boars Publirzikm Advances in Blochemical EngiraarinpE alg hron Authors Jahn W Vilncdley Publisher Eprinperverlag Heidelberg Recency vclume Oram Rekrvancy 25 5 Pees Pee omo m oom oum om ss a a a oom cm e eee m aw c iom cam cn cm om mde moa bee eee Laman Fassavrd C fernir ita nn one Dess E SE Number of hits Tio 16 of f st 1900 ene Filter option Last page Filter Seibel Iba Search Within F suh Sear Shin ihiee mute For Sesech Search within the search results Canv eht 7004 Sarincardink pase Back to Table of Contents C ack to Table of Contents opyright amp 2004 SpringerLink All Rights Reserved Page 42 User Manual Version 1 00 3 3 Advanced Search SpringerLink T der RA Advanced Search searches for more i Jr an
16. ea Fe Lari amean rede in PD nimai or questions We will For Our Hen Cuslomers F r Sal he 1 lSren sten au need Do Ga mi ne our SEN wai S nngerLirk pease kilos Ins Ink fo Ine FAG page respond aS SOON aS possible Far Our Either LIBRE ahi een Bhar mas be und or u FAG pane Fenr Ali ih Springer kemad alerts user cam ragleter a Kesar and gach tima the kored appears in pudlicaban om EpringerLink fee use will nere fer an nales TRE Rare heks taithi be me fre khe st r nemesis malabis online For assistance inside the Americas zprnoerimki e pningges mr oon For assistance outs kee the Am eric ac Seinen ps e Springer Vek healer Temaet 37 C amp 31 31 beideskeig Oemnsry Prksov Dincbserer Tero and Conditions 0 Copatki intonation In addition there is more contact information not listed on the website CHINA HONG KONG SOUTHEAST ASIA SOUTH KOREA Maurice Kwong E mail mauricek springer com hk Edwin Chu E mail edwinc springer com hk Grace Lau E mail gracel springer com hk opringer Verlag Hong Kong Limited Unit 1702 Tower 1 Enterprise Square 9 Sheung Yuet Road Kowloon Bay Hong Kong Tel 852 2723 9698 Fax 852 2724 2366 E mail springer springer com hk Canv sht 7004 Sennemd inb 5 mec Back to Table of Contents C ack to Table of Contents opyright 2004 SpringerLink Page 67 All Rights Reserved User Manual Version 1 00 TAIWAN Leo Lo
17. libes her need Browse and Explore 5e Ingen Login vin athena Bro se Publicbers Hyoa are already registered pic asn legin Kyou are not sure we can de Pubicaflions in Sprimpes Link hrin 5purgeiLit keselcomes ne fol cosi nes ies m bt el Tat here sep D bec Ucsmname aden 1o our collection cf online pusbcalimmz T NEN Creo and hy Fasswmd Cognitive Process meet pm nm R er Va Es ee uz PE Burgin Jousnal of Fores Aese ch i Baropesn Joumal of vele research Register E yopesn Firing Tuppits Register ve Fhegisiering 15 simple and tee For nro E Pini Mallon amp New Publications in iain o DOE P i al erbe ad Lr riari SpringerLink al Dering Register l st Son gef i orari ol Pita Head Lada s Retro of k lbenl es bersetak eat mante Linking O ptions Linton Options Paasa pk h re Bo reo mno 5 end od ou nos les AO Springer Lik Brochure Mos Arailsblr Spingsturk Erochire Enh Spingert irsk Bronse Cestaril To quest prirtversions of he broghures please conlacl kool penger ny ccm Hirka spe ce For addicnal irfoeinaton abut SpringerLink please see Ike Faro page Download SpringerLink Promotional Materials Laniot Bornstein Index Vea Tha Lari Plon ness in POE emat Far Our Hew Custnmnmers For all of ie sifammalbion you need tania wie sts Sardar Seas Far ur Panbnrs Ce 30040 electronig publicatis DE EE be byrd oci dg Fez page Keyword Alert Keyword Alert wir ap
18. titles found at the top right hand corner of the page It is the total number of book series and journal titles found Copyright amp 2004 SpringerLink Page 22 aCK tO ladle OF GONTENtS All Rights Reserved User Manual Version 1 00 2 2 Browse and Explore opringerLink provides users with three options for browsing the contents Browse Publications Browse by Online Libraries Browse Publishers Browse and Explore on the home page of SpringerLink Browse and Explore With a collection of journals and book series that account far over 300 000 documents in SpringerLink the browse and explore functions help users to get quickly to the information and titles they need drow se Publication Browse by Online Libraries Browse Publishers 2 2 1 Browse Publications A click on the Browse Publications option will display titles in alphabetical order Users can click the corresponding link to get the desired book or journal contents Details of browse operations are listed in Section 2 4 Viewing Publication Contents Springer Link r ay CN Sr M NPR OSE T Gf LEIP SS ORDERS PROFILE 0 gt Home Navigation Browse Results help 69 251 JARO Journal of the Association for Research in Otolaryngology 251 to 255 of 525 Publisher Springer Verlag New York First page x 252 Journal of Anesthesia Previous page Publisher Springer Verlag Tok Went pa
19. vU scade aim ga De eo r Hane i let oa What s New on SpringerLink specific results Ain Aouri ka Mo Vilaitiesg You are bogged in as krblipie User of Scientific Unkwersihy Payer ew maiae an Swing i oak Due io an ove ryhelming demand fom Ihe research commun s SenngerLink nasr Your ineliiution s MetaPress I rs Diera pap pede pptipn for jaumal and Same Ecaksaries articles This option I3 450578 noens He door to expanded resesrch and removes He boundaries nir guiar bera oen lare Pan per aeer is avaible lo reg sierra users oF SprieigerL ins Cusiormers To use Ihe personalized features of use Secure or standard purchasing and il hen be able a vere purchased areas tig site sux as Fanas o Table artine through Feir Onder Halo of Contents Alert you should login or register as an indredual use Drovsan arid Expl feu will stil hawe accpzz In all the With a collection of Mumak and book seres Teal account Tor wee 300 000 suste adore aealatia farough you documents in SpringerLink ha browse and mpare Tonctipniz help users to gal samia or insiuson quick to thee intonation and titles they nesd Bree Pubic alors Login Login sia Alferd Brosse ky nbn Lie ankes Browse Publishers ru ae aleat moktered please gyei Py are rol are we ran Meer Publications in Springer ink Springer nb ee ere Na m r a new pes in hil led Hal bare recenti been added ie our colercban of orine publications iratra T Cngrdev
20. 0d Abstract Far more than 30 yearz the only enzymatic system known to catalyze the ebrination of supercode was supercode diemmtase SOLD SOLD has been cord zi almost all orgasms Eug un the presezce of oxygen uciudng some anserchuc bactena supporte te notion thal suseromde is a key and pacera component of oxidatree stress Kecen a new concept in the Beld of the mechanisms of celhlar defense against zuperoxide has emerged It was ducerered that dimeshen of sperare gi some anaerobic and mecroacrophibe bacteria could occur by reduction a reaches catalyzed by small metalne Hs named sperod reductase SOR Having played a major rode in Hus discovery we descobe here how the concept of superoonde edoren exezged sd how tt was expenmentallz substzenated sdependenty m ov laboratory Eeywurdz Coidatye stress Supreroxde Superomde dismutase Suneroxide reductase Introduction Eyben monooxygenzse AAND x membrane spaening non beme diron enzyme with a hisb ine nch active site that catalyzes the insertion of an oxygen atom mito a C H bend 1 Ay har 2598 sequence heanology with alcant moncoxygenase LAIKE 2 wiih sigrbeant homology i beth a eulhi hisi ne mett aed nthe presence of three long hydrophobic domare thought bo be membrace spanneg regions 3 A detuled comparison of the anno acid sequence of enr of edrosvlases suggests that Ayh and AKE together with a number of qmembrane spaening desaturases form a third disini class of duron enz
21. 3 haem copper oxygen reductase comparison Click here to access cus demtriicams aa 3 haem copper oxygen the article ares Ant nio M Baptista Manuela M Pereira et al soo is 75 03 n5059 9 By clicking on the link users can view the contents of articles listed in the table of contents Article Full text document in PDF format Previnus article Journal of Biological Inorganic Chemistry Kiert article Publisher Springer Verlag Heidelberg ISSN 0348 8257 Paper 1432 1327 Online Linking Options DO 10 1007 s00775 003 0466 3 Full Text Available e EMI The full text ofthis article is available Date September 2003 available You may view the article Pages 733 740 as ay PDF The size af this documentis 309 Kilobytes Although it may be a monooxygenase a membrane spanning non heme diiron lengthier download this is thy authoritative online format Open Manaa ug One page at a time in 1 1 5 in Kim 3 4 EJ Sc IEDNE Only one Austin Kate Buzzi Eungbin Kim Gerben J Zylstra and John T Groves Local HTML Non Paginat Full text This offers the quickest acces tment of Chemistry Bates College 5 Andrews Road Lewiston ME 04240 USA ease of browsing Please nate tA page of this some scientific and mathematical mE characters may not render as hnalagy Center for Agriculture and the Environment Rutgers Univ
22. 5 6 Tobe ML Khokhar AR 1877 J Clin Hematol Oncol 7 114 137 ChemPort 7 Bramwell vHC Crowther D O Malley 8 Swindell R Johnson R Cooper EH Thatcher M Howell 1885 Cancer Treat Rep 55 408 416 ChemPort PubMed Reference linking options take users to supplementary materials eg diagrams or lists of references Copyright 2004 SpringerLink Page 38 ACK tO lable OF GONTENIS All Rights Reserved User Manual Version 1 00 3 Search Function The powerful search engine in SpringerLink offers three levels of search functions to meet different readers usage habits and requirements 3 1 Searching within the Publication opringerLink provides the Quick Search function within a publication selected SpringerLink 2 amp Hari Bror Fesil d Publication Journal of Biological ner gane Chemistry Pubic ation 253 of 524 Publisher Springse Vesiag Hedalber Prewiows Publication EE SSM 0045 8257 Paper 1422 1227 d mine Type the 4 Susjerb hirci Sainai Lie Sdeoai Linke Option keyword s and Aliud Thig Journal raiariat nara press Search ihanuscripi Submission rutte xi brodd Sora det S bct l ou are billed 15 vira button dine Firs Quick Search Volkes D march within this publication Humber 2 4119 245 For enzyme Humber 1 1 118 mam Wokare H Humber A 803 907 Franken Fil Humber 7 26989 Bl Cic the bution below io add this Humber 6 505 697 puaicabnn
23. 55 mlenstonal Jounal on Software Toots Tur Technology Transfer iSTTT Latt page fo 1506 Imonstaonal Microbiology ex 152 Intinnarional Orilmpasdles e 150 Der Incemisi de 458 imeentiones Balhemalicae S 460 Imerivbrate Neurnscience u 331 Irrigation Science cu 182 MIS Journal of Unkerzad Computer Science e 183 Join gorgac al iiine e 484 Journal ol Cancer Reseach arid Clisical Oncology Jounal p Comper a oe Piysiology Sensory Neural and Helraryineal Click the title link to get the contents an AAG Copyright 2004 SpringerLink Page 25 ACK tO lable OF VONENtS All Rights Reserved User Manual Version 1 00 2 3 Online First The Online First service lets users access peer reviewed articles well before print publication significantly reducing the time it takes for critical discoveries to reach the research community Before any article is printed they are published electronically in SpringerLink and grouped under the Online First listing When the article appears in the print version of the journal they will be grouped under a printed volume issue and removed from the Online First listing Please refer to Section 2 4 Viewing Publication Contentss for more details Copyright amp 2004 SpringerLink Page 26 ACK tO lable OF VONIENtS All Rights Reserved User Manual Version 1 00 2 4 Viewing Publication Contents Users can click the publication name on the Brows
24. 6 kilobytes Although it may be a ad tase an historical lengthier download this is the most authoritative online format e E and Marc Fontecave One page at a time Local HTML Non Paginated This offers the quickest access for ease of browsing Please note that some scientific and mathematical characters may not render as nreriselv as in PDF versinns Minireview Click this link to get the Linking Options of 1 Laboratoire de Chimie et Biochimie des Centres Redox Biologiques DRDC CB UMR CEA CNRS Universit Joseph Fourier no 5047 CEA Grenoble 17 Avenue des Martyrs 38054 Cedex 9 Grenoble France Bacanzard 72 lik NN rrantart 15 Naramhar n60 Diibliehar nnlina this article Linking Article Link To link directly to this article in volume 8 Number 2 af Journal of Biological horganic Chernising vau may USE Q MetaPress Direct Linking This linking scheme requires an internal object identifier assigned to this contribution by MetaPress To link with the MetaPress object identifier use http Magna springerlink comdink asp7id thOhcfjf mdftyql Q OpenURL This linking scheme is based an Open JAL It allows you to encode citation information for a resource in this case a contribution as URL To link with the Print ISSN use http An ww springerlink com openurl asp7genre article amp issn 0949 8257 amp voalumezS8 amp iszuez2 amp spagez1 1859 To link with the
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30. Kylld pdroxylatez hydrocarbons via an oxygen rebound mechanism amlar to tat used by the presumably structurally sumar enzyme AIKE the resolution of the data more than adequate te answer that question alirmahvely arkeme far the reaches between Xylk and norczrane is represented in Fig According to thea model the deren site on SIM reacts to form a high valent pecrecbabe analogous 16 compound G nm sb bdo Thus specs 5 then capable c abstracting hydrogen stem fom nercarsee formmg carban centered radical whose stram selieing ena openng bakes place B conset with the ezzyme mediated OH rebound step Cationic products are proposed to anse from the abstraction of second electron frem the triersent carbon cezitezed radka KK E Xe C Page 37 ACK tO 1ADIE OF UONTENtS opyright amp 2004 SpringerLink All Rights Reserved User Manual Version 1 00 References 1 Rosenberg B van Camp L Krigas T 19651 Mature 25 588 699 2 Rosenberg B van Camp L Trosko JE Mansaur vH 1969 Mature 2227 385 3 B 3 Braddock PO Connors TA Jones M Khokhar AR Melzack DH Tobe ML 1875 Chem Biol Interact 11 1 45 161 Leser cent 4 Kidani Y Kizu R Miyazaki M Maji M Matsuzawa A Takeda v Akiyama M Eriguchi M 1885 In Pinedo HM Schornagel JH feds Platinum and other metal coordination compounds in cancer chemotherapy 2 Plenum Press New York pp 43 51 5 Cleare MJ 1977 Clin Hematol Oncol 7 1 2
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36. ate radi cal was determined to be approximately 0 2 ns The wild type organism Pseudomonas putida mt 2 and two separate Escherichia coli dones expressing xylMA genes gave similar results One clone produced the Pseudomonas putida mt 2 XyIMA hydroxylase and the other produced Sphingomonas yanoikuyae Bl XylMA hydroxylase use in selected parts of the global carbon cycle without requiring the isolation of every protein involved Keywords Cytochrome P450 Hydroxylase Methane monooxygenase Non heme diiron enzymes Radical clocks Abbreviations AIkB alkane monooxygenase CYP cytochrome P450 IPTG isopropyl f D thiogalacto pyranoside sM MO soluble methane monooxygenase XylM xylene monooxygenase Introduction Copyright 2004 SpringerLink Page 34 aCK to ladle OT VONtents All Rights Reserved User Manual Version 1 00 The following is a sample of the full text in HTML format JBIC Jeonal of Biological ergai Chemistry amp SPIC 2004 10 10075500775 003 0519 7 Minireview Discovery of superoxide reductase an historical perspective Vincent Nivi amp re 77 and Marc Fontecave 1 Labware ea Chimie cf Biochimie das Cantras Rados Bielagiquas DIBDIC CB UNS CEA HREUnsersit Joseph Fourier no 0H CEA Grenoble 17 Avenue des Marlyrs 38054 Cedex 9 Grenoble France E3 Wincor Nivi re Email 5 Phone 4 09109 Fax 3 d 39760124 Received 26 July 2003 Accepted 15 December 2003 Published online 13 January 20
37. ble of Contents opyright amp 2004 SpringerLink All Rights Reserved User Manual Version 1 00 ur Fig 2 Strucnees of reacarane ird nercaraee sedan products E e 7 fit te madal Jamia Fin Fig TD Duztrisution e preducts fom al three mood First block m each setis znt Z cene wlatey second block s EAM close back thd block zs wid type adr gea beck aud wire siriuge Error bars represent standard errors sac D Ceara sement conce d har the chenustry detected war due to the catalyec active of selene moncaxygenase and not due te spurious alene or bioti oomdahoes Ho products were seen when noccarame was mcubated for 12 15 hin buffer Mo products were seen when norcarane was smcubated with a negabve contrel af 208 contarmng cay the espprissen vector with no menocxygenaie gene Abice expermnesta were also done te test the stabibry of products te the experzzental workup conditons and all products were found to remam umodbed by rcubaticn m buffer followed by extraction and MS analysis To serere that product quant c ation was not compromised by bather metabolem of the mhal products we snthenzed 2 norcaranone the correspocdrg ketone for 2 norcaranal 3 morcaranone the correspondeg ketone for 3 porcaranoD 3 cgclohepterone the comespondng ketone for 3 cyrioheptenoD and 2 evelshetene cacbaldebyde the cormespondng aldehyde er 3 hydresmelhyleyesherene and lesked for tasse molecules m well m the GC MS chromatograms Whe
38. ccount for over five million articles in over 8 000 journals Click on the SpringerLink icon to activate the crossref function which will link you seamlessly to the cited article on another site If you are also a subscriber to the service of the linked publisher you can have access to view bibliographic citations abstracts reviews or even full text seamlessly Copyright 2004 SpringerLink Page 59 ack tO 14016 OT VONteNts All Rights Reserved User Manual Version 1 00 Thus in order to get the reference lists users can click the reference linking icon in full text in HTML format SpringerLink will display the following screen References Gabler B Schmid A Hauer B ito E 000 I Biol Cham 273 IOCES TUCEXZ 2 Suzuki M Hayakawa T Shaw J Rakix M Harayama amp 1991 J Bacterial 173 1690 1688 terre Pebl 3 Shankin J While E Fax EG 1934 BiGzFemm Click this icon of abstracting and Cee Pret PubMed indexing service for access to the 4 Snits THA Pioch isberger M MVerhak B van Halan JE 198899 Erann Microbiol 1210 317 era E 5 Rlener A 1992 Angew Chem Irt Ed Eng 31 774 775 bibliographic data and abstract that may have a link to where the full text is hosted B Panke 5 Meyer Huber CM bak B Vobbaks hii 15699 Ape Emeron Nicrabiol B5 23245 2332 amp her m Fori 1 J Leahy JG Colwell RR 1990 Microbiol Rev 54305 715 Cla ii Pari Publ ChemPort PubMe
39. d Harayama S Fishira H Kasai Y Shut zu 19885 J Hal Mizrobial Biotechnol 1563 70 8 riz de Montelano PR fed 15995 Cytochrame P450 structure mechanem ac binchernisiry 2rd edn Plenum Hee York Back to Table of Contents Copyright 2004 SpringerLink Page 60 All Rights Reserved User Manual Version 1 00 Via Reference Linking you are now linked to the data of the relevant article from collaborating partner sites Depending on if you are also subscribers or what kind of subscription you have in these services the level of access to the contents data varies Heme Help Contact Lee Welcome to CAS from Springer Verlag LIMK Journals Explore with SciFinder der Customers e CABGIndexed chemical substances from tis Socument There ana 198 citing EE C documents SrF der Scholar Customers alor me moret Eight Histidine Residues Are Catalyticalhy Essential in a Membrane Associated Iron Enzyme Stearoyl CoA Desaturase and Are Conserved in Alkane Hydroxylase and Xylene Monooxygenase Weew Full Text View Full Text Opsioms Shanklin John Whitte Edward Fox Brian G Biochemistry 1994 3343 12787 9d CODEN BICHAWE SSA 0006 2360 English The eukaryote fatty acid desaturases are iron containing enzymes that catalze the NAD P I and O2 dependent introduction of double bords into mettrlene internupted fatty acyl chains Examn of deduced amino acid sequences for fe membrane desaturases from mammals fungi
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42. ed User Manual Version 1 00 The following screen is the confirmation of the keyword alert setting Users just need to click the Finish button to confirm a SEARCH FOR RETURN SPRINGER FH x 3 d ADV Publications ies SEARCH gt Home Keyword Alert Click Finish to complete the Search Results Criteria enzyme 1 to 10 of 804 keyword alert y 1 The honorary enzyme haemoglobin turns out to be a real enzyme Next page Previous page Publication Cellular and Molecular Life Sciences CMLS Next page Authors L Lebioda Last page Publisher Birkh user Verlag AG Recency volume 57 Numbers 13 14 Click Finish to finalize Relevancy 32 0 f this Keyword Alert or Excerpt This article does not have an abstract Modify to change any criteria and resubry 6 2 Enzyme to enzyme channelling of symmetric Krebs cycle intermediates in iii Click Modify to pancreatic islet cells Publication Diabetologia te OG return to previous Authors V J Malaisse L Ladri re T M Zhang et al Publisher Springer Verlag Heidelberg Recency volume 39 Number 8 Relevancy 32 0 Excerpt Summary Tumoural islet cells ofthe RINMSF line were incubated for 120 min in the presence of 2 C propionate 10 mmol l and the 190 enrichment of lactate released in the incubation medium was screens to modify the keyword setting Pr SEARCH FOR RET
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46. ersity Mew precisely as in PDF versions e PE A rtment of Biology Yonsei University Seoul South Korea article in PDF format is shown at document in HTML format available Open Full Text a time Copyright 2004 SpringerLink Page 33 ACK tO lable OF GONTENIS All Rights Reserved User Manual Version 1 00 The following is a sample of full text in PDF format Ca Acrobat Reader BQEYHGYB99NGHYDQ pdf DASA ME JD g me Bor nl RH Fn IT as EIL 70000008 AMERO ce en MEN na oe ed PI ER 8 T J Biol Inorg Chem 2003 8 733 740 DOI 10 1007 800775 003 0466 3 Gs amp NORE 8 ORIGINAL ARTICLE i ARTICLE Rachel N Austin Kate Buzzi Fungbin Kim Gerben J Zylstra John T Groves Xylene monooxygenase a membrane spanning non heme diiron enzyme that hydroxylates hydrocarbons via a substrate radical intermediate Received 24 January 2003 Accepted 11 April 2003 Published online 14 June 2003 SBIC 2003 Abstract The non heme diiron enzyme xylene monoox ygenase XyIM has been shown to hydroxylate hydro carbons via a hydrogen abstraction carbon radical recombination mechanism oxygen rebound Using the radical clock bicyclo 4 1 0 heptane norcarane in a whole cell assay and observing the ratio of rearranged 3 hydroxymethyljcyclohexene and unrcarranged 2 norcaranol products the lifetime of the substr
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57. insects higher plants and cyanobacteria has revealed three regions af conserved primary sequence containing HS or diH H ar 3 FH and HYY or SHH This motif is also present in fe bacterial mermbeane enzymes akane hydroxylase m hydeoxylase and xylene rmionooxygenase Hydropathy analyses indicate that these enzymes contain up to Eight Histidine R sidwes fue Caralytically Essagiial im Membrane Associated Iron Enzyme Saan CoA Desaturasg and ISSN DOO Eni Hydroxylasp and Sylome Monoosygenase Biechemistey 1994 33 23 12787 94 CODEN EIC HAM I Here are the options fos the document you requested l Webbased document resources fan Fee based document services Download Dacumgni Authorization Required von host payee tied an fer fom me 205 Pune done Ve Dermei Pal ree Sale len cede x Eker HD and F as eng n P san Ing bio cepe Bree ey AR Important Notes 4 Thi tere zz 245 2 rl zcadrcnakiesc 1 cecmriraling 125655 is Secure dezuemmenieizanmiraz Ha persona pi carne retaliated x Pe coke ard Hae cooke 5 odexsled ihehnenai cil TW Ercmr er In balls yer User D and Fa coer ae bals sel ic aurcs Mercer Humber cr fcu a erala n amber ho your sudbeo kar number Forinfomaden on Indira cog memlbbieczsulEscriter rember The mets Edibu ns of the aS SU AG Ue epee eutatcngi 6 hen he geni suylhecnplon or amine Stress Feces an subscrip irfimimmashnn Ha ACE puslrstans click here Questions or Comments 4 Lorn ered
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62. n detected they were inchsded in calculation of the rako of nng closed to ring copen prodacts used to determene the radical hfetime of the substrate In addition potensal product were incobated with the cells ar sussteates Alcohol were seen te be ransformed gradually and partslly to the expected ketones over the coarse of a 15 b mcubahon The we of two cod hosts expressing where monooxygenase whose rezltz are te same as the wild type cemere allow ua uneaurrecalh te adios the cherishes brno cheered t Xulkd The rearrangement rate For norcarane has been determined to be 2 109 a7 34 For Xs M we calodate a radical lifetime of 0 2 10 s for each of the tese orgamsmns bested the Brenta for calculating the radical bfetene from the metabolism of a radical clock z radical ldetime oem Hrantrns gj rnen a Graanbfir ation was based on maraa integraboes of all product peaks and crosschecked with be intensity of key ions for each product Standards samples of all product were smibessed Chromatograms fom whole cell extract sen conten multple peaks occanonaly co ed of peaks presents problezn Compancon of both guantbrabom approaches showed that differences n results m be two approaches were small For al three organisms the average radical leere of 0 2 ns had standard error o 0 04 p organum spec x standard errors are ar EoDows mi 2 clone 0 075 na Bim 0 1 n wt 0 185 nz Since the primary question beng posed in Hus work iz whether
63. norganic amp nuclear 2002 Rank 7 af 45 Impact factor 34 Abstractedindexed in Chemical Abstracts Service Current Contents Life Sciences Index Mi Fuhhed Research Alert SciSearch Science Citation indexed Editorial Board Contact of Editorial Board of this publication societies Description Editorial Board Editorial Board Chief Editar Lawrence Que University of Minnesota Department of Chemistry 207 Pleasant Street SE Minneapolis MM 55455 0431 USA e mail jbic rchem umn edu Tel 1 612 626 9405 Fax 1 612 625 0078 website hip Mas chem umn edu jbic Manuscript Submission Societies that this publication belongs to Societies Description Editorial Board Societies Society of Biological Inorganic Chemistry Copyright 2004 SpringerLink Page 32 aCK to ladle OT VONtents All Rights Reserved A description of the aim and scope of the journal and its contents Information on where the journal is abstracted and User Manual Version 1 00 opringerLink displays the table of contents when a certain volume issue is clicked Journal of Biological Inorganic Chemistry Issue 2 of 52 Publisher Springer Verlag Heidelberg Previous Issue Navigation ISSN 0949 8257 Papen 1432 1327 Online Next Issue issue Volume 8 Number 2 Date March 2004 Articles 1to 13 of 13 help Linking Options tion of protonatable residues in RAroedothermus pp 124 134
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68. ric peroxo species 22 have also been suggested Conv sht INNA Sensedink 5 55 DPasas Backto Table of Contents C Page 35 ack to Table of Contents opyright amp 2004 SpringerLink All Rights Reserved User Manual Version 1 00 Catalyt acbviy of X SUM bowards norcaraee X uM enetsielimed marearime welding distribution af rmg desed and zng epen products Cenerady 2 7 4 ofthe neccarsese was metabolised 16 products over the course of the reaction as determene d by qaantibrey the moles of products knowing the moles of z3ostrate added and taking Eto account be extraction efficiency ef the aleshols Fesi the reached meme This convertion rate ig cocten with ries seen m other whole cell assays Unrecorded orcarina vs difficatt vo ganii alter the reaction because the olankrg of norcarane causes x to be lost dueg workup For both the wild type organes ard the mr 2 XL and El ANIMA clones appeornsately 87 7 2456 of the zorcaranc dermed products are emda gd exo Z norcaranol compounds d and 5 respectuely and 2 norcaraneme compound 9 Approsamately 3 9 4 54 of the norcarane dermed products are 3 kydrosymetbyliryelehesene 2ompoued 3 the nng cpen product arena from a radical precursor In many experenents small 1 7 26 amounts of 2 rycloheptenol compound 3 the bydkorylaten product fam the reg op med cahit resonance farm were seen Faure to detect iu m all experunerits ray represent te Fact that levels m some experrzent
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72. s may h ve been near the detection limits of the assay Smal anvounts of products from oxidation at the mechanistrally menformabve 5 posison were seen wath some organisms chromatogram frem typical reaction is iawn m Far 1 Table show the average product ciztbumons frem all asperitats for each organism and the standard error of the values obtained The data represent the average of four oc Gre diferent eqperments with each stram When the wild type organisms were grown woh meetin ent o bsylnethaeeos was produced Figure 2 shows the stnocbares of the various ceamounds and Fur 3 the product distabunea oo Mylene Monogxygqenaze amd Hocama Wik pe at IDOL noram iri 252 ril growing calls E ms E Ten 3 9 D ag 70 71 72 73 Ta r minies Fig 1 Eeprezentapve GO MS mace for KYylk condation of norcarane Table 1 Distribution e products Bom Xy oxdaton ef nore ane average t SE Data gren in terms of tetal norcarane dermeed productie 2 3 4 5 9 yi 3 vet mte 27 005 3 9 0045 73 1 6015 17 1 265 3 2 2 75 0 mi 2 cene 1 140565 4 301259 633058 1863 35 12103 33b 10054 Bites 2208 4505 62638 19 34 G18 L5015 13003 amp compeoud amp mdo 3 porcaranol co elutes with 5 oed cannot be precssely resolved although the Gagmentation pattern s clearly dominated by 5 7 exo 3 nere ranet can be resaleed gt GA gt OO DoD Canvrioht NN Seunsed inke basa ak Backto Table of Contents C Page 36 ack to Ta
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76. species of fungi that can metabolize alkanes 7 9 have been 1dentbed Whether that number represents the mayenty of hydrocarbon transforme spaces or mranuscule cdburable fraction i not clear Byen lezs is known about the cererzity of metaloenrymes that can catzbyze be monooxggenation of hydrocarbons The growing illus of geese dala presses demmet 15 ask md Gnd ways to address questione regarding the diversity ol Edroxytases by rapid and efficient procedures Vti recently the pure saradigens fr biological hydrecarbon oxidation have been cytechronme P450 CYP heene contagung presen 2 md MMO a non hem dmon enzyzne i D bore than kyo decades of mechanistic stades on CEF and metaloporphyrm models have led bo a consensos mechanism involvrz the binding ied reduction of desygen to form Erst a gerome FerTIT state and vitimately an oxo iron IV porpb re x cation radica compound T as the acte oxygen species see bor example 17 2 13 24 Ths fered species was recently observed m Cyp 119 a bermepble 2 Wide stopped Bow beem quench experiments with PESO baled to detect a Opd I abermedate 75 the substrate derreed alcohol haz been found at low temperature te be formed Wh the hydrosyl oxygen bound te the bere won 77 Hydreaylition it believed te occur via hemolyer bond cleavage of te C H bond Followed by OH rebound to the carbon reztered substrate radical 8 19 20 Cahome processes 27 and electrophdic reactivity of the er
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82. with zubzTalez and carboryl reaala of nali bold yearns caidase and its modifisert freme Le Capri depleted Cy hakrecorsialed Cyu tlk resonsinsled C substtuned Hi subtubedand Zr zubsklused has been studied Upan removal of oet ane of fa Wo Cu lons a 2 Crystallographc determination of reduced aine supercode dizem asse al pH 50 and af anion Binding in its aczive sir Publication Journal of Biplagiral Inzeganir Choir Authors Marta Farmari Faojclech A Fires Bruno Bruni et af Publisher Sprinaar anag Heidelberg Recencs Volume 3 Humber 4 Relevancy 7 0 Version 1 00 110 18 of 22 Kad page L st pope Esarcn Ain Hesulls Search within hase results Fite To confirm the validity of the search the keyword is highlighted in the search results Click on the article titles to view the abstract and full text contents Conv sht NNM Seunsed ink based Backto Table of Contents C Page 41 ack to Table of Contents opyright amp 2004 SpringerLink All Rights Reserved User Manual 3 2 General Search Version 1 00 The search engine also offers General Search on each page It searches for all publication contents in SpringerLink under the following options Articles Publications Publishers uates Options in General Search What s New on SpringerLink Ho Eimear ka Mo Vain F rre ver mer Pabe au Spinqert k Due Ip an overyhelming demand tom the research community S ringerL
83. ymes volutonacty distinct classes beng typdbed by hemerytinn class D and soluble methane monooxygenase SM MC class ID 3 genetic survey of erot ug dezrading bacterez showed extensive homology to the sj ht hist ne mott of AKE and X 30M farther indicabng a widespread role for ilu inet the aimait 4 Xybd har been used both m the maisi cogan fer the commernal scale production of hetercaremanc acide 5 id m iei akame responsive expression system in Ezzhericka col for the moderate scale comsersion of styrene to S1 styrene conde EL Reference Linking The mzertion of oxggen into a C H bond is a cbemical reaction that bas long fascmated chemests both because C H bonds are dificult bo dsrupt and because omen xs difficult to seecheey active Hy scarbsa represent an enengy nch fuel for microorganesens The reacten bekeren molecular exygen and hydrocarbons to yeki an alcohol and water 15 a Hermodynsrrec aly Evrorab e reaction genezanng products that can ultimatelz be fed mto aerobic respiratory pathways In spite of the thermo dynamically favorable nazane of hydrocarbon mygenaben kinetic barriers and other challenges e g the ask to the orgamsm of producing rescbve oxygen species constram tus strategy Thue a brzibed manber of srgsnsme exist that can grow on hydrocarbons as ther sole some of carbon and energy Just how l reted that number of crganizms is mot known Aperommately 100 different species of bacteria and 100 different

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