BSL 3.7.5 PRO Tutorial
Contents
1. delta l O 07 mv BF Mid 7 57 BPM P P 3 2 96 mv deltaT 4 7 93 sec P Pi4 0 99 Liters delta l 0 16 mv BF Mi1 7 51 BPM P Ptsj 5 12 mv deltal 4 7 99 sec P Pt4 0 56 Liters PRO Tutorial 23 One advantage of saving data files to disk is that you can quickly and easily perform post hoc analyses of your recorded data BSL PRO software is a powerful and flexible analytical tool designed to provide you with immediate feedback from each operation Using BSL PRO you are able to Use digital filtering and smoothing Find patterns within data sets Automatically find peaks and calculate rate data Perform mathematical and statistical operations Log results and observations to a journal Mark events during acquisition or analysis Transform data after it has been acquired To get an idea of how BSL PRO provides immediate feedback run a Find Peak function on the 4Channel acq sample file Select CH1 and hide the other channels by pressing Ctrl and clicking their channel number boxes Use the Zoom tool to examine a 10 second portion of the waveform To center your display after zooming use the Autoscale Waveforms Vertical command from the Toolbar or from the Display menu SiS eee ies HER EE z O E ri BIOPAC Systems Inc www biopac com 24 80 Choose Transform gt Find Peak 8l 82 to generate the Find Peak dialog Choose the Off line Averaging option in the2. BIOPAC SS2LA 2 Pulse pressure PPG BIOPAC SS4LA 3 Heart Sounds Stethoscope BIOPAC S530L 4 Respiration RSP BIOPAC Ss5L zi Drag to resize journal size The BSL PRO display consists of a graph window and a Journal Adjust the size of the Journal by dragging the bar that separates it from the graph window You may resize the entire BSL PRO window to best fit your computer screen by dragging its corner or elsewhere on its perimeter As you practice using BSL PRO tools and commands you may substantially alter your sample file If so you may choose to close it without saving and then reopen a fresh working copy to continue the Tutorial You may want to practice on a copy of the Channel acq sample file That way you can experiment with BSL PRO without worrying about changes you may make and save to the sample file provided with the software installation By default BSL PRO data files are saved in the BSL PRO format with the acq filename extension Saving a file in the BSL PRO format saves the graph data and the journal notes the setup parameters established under the MP45 menu and window positions Except in exceptional cases you will save data files in the default BSL PRO format ACQ File name 4Channel_T est acq Save as type esL PRO files ACO ISL PAO files ACO Graph Template 4TL All BIORAC Files ACO Ldd TT GTL IMPORTANT Saving as a Graph Template does not save any data only
3. gtl A Graph Template contains no recording data but contains the parameters that you have set up and saved with the file When you open the template file it is ready to record This is useful for creating your own lessons Consult the BSL PRO Manual to learn more about Graph Template files Calculation channels acquire data from analog input channels or other calculation channels and perform a calculation on that data The original source data is not altered it is mathematically transformed and stored in a modified form in the new calculation channel An important concept to understand is that calculation channels are not input channels They must ultimately acquire their data from an input channel that collects signals from an external source When the source of a calculation channel is another calculation channel that calculation channel in turn must acquire its data from an input channel usually an analog input channel though possibly a digital input channel www biopac com 10 24 Click the Presets icon 25 26 27 28 down arrow for Calculation Channel C1 and choose the preset option ECG R R Interval _ Click the View Change Parameters icon wrench to the right of Calculation Channel C1 to generate the Rate Parameters dialog Note the Rate Parameters for the ECG R R Interval calculation and read about them at right Click Cancel to exit the dialog without changing rate parameters and r
4. the setup parameters PRO Tutorial Part 1 Acquisition Parameters SET UP CHANNELS 10 Choose the menu command MP45 gt Set up Channels to generate the Set up Channels dialog Data Input Channels Analog Input Channels Calculation Channels 11 Read about data input channels at right BIOPAC Systems Inc Each BSL PRO lesson including experiments that you may design will have unique procedures for attaching electrodes transducers and other signal monitoring equipment Signal monitoring equipment is connected to the MP data acquisition unit and the MP unit in turn is connected to your computer Follow the instructions for your particular experiment to set up the hardware Before recording however you must set data acquisition parameters in the BSL PRO software The Set up Channels dialog displays options that determine which channels receive data what type of data the channels receive and how data is displayed and interpreted on your computer Ml Set up Channels X Channel Acquire Data Label Presets View Change Plot on Screen Parameters Enable Value Display ANALOG INPUT CHANNELS CH1 w m ECG lead 2 ka ae CH2 Iw fe we PPG 5 35 Hz ae cs WW H e c PEE 4 r D1 Emp D1 Digita w rrF H AEE D4 mimm D4 Digital Input 4 r CALCULATION CHANNELS Ci E el I C1 calculation OFF cz Sl eI C2 calculat
5. ANALOG INPUT CHANNELS Note CH3 and CH4 data is l Hi feliei ecGlead2 displayed in the sample file but the set up options are CH2 w v Iw PPG 5 35 Hz not selectable greyed out CH M ef Stethoscope Heart Sounds because the MP45 supports only two channels cHe i Iv Respiration Clicking View Change Parameters _ generates the Input Channel Parameters dialog for each channel which allows specification of settings for gain offset and other recording parameters 16 View the Input Channel EUS EE prereset Parameters dialog for CH2 Channel Number CH Channel Preset PPG 5 35 Hz Channel Label PPG 5 35 Hz Digital Fitters Hardware Gain Offset Input coupling Filter 3 Type Band Stop f AC i oc 0 05Hz HP O5SHzZHP 5Hz HP MP35 36 45 0 1 kHz LP MP0 5 kHz LP PSO Freg 0 0000 Hz Q 5 00000 New Channel Preset Scaling Cancel 17 Read about input channel The Channel Preset for CH2 is PPG 5 35 Hz The Channel Label is parameters at right not always the same as the name of its preset parameters you will often label a channel differently to better describe its data Gain Gain specifies the extent to which an incoming signal is amplified Preset gain settings are educated guesses for the data type selected and should be used as initial starting values you may need to adjust the gain depending on how the ampl
6. MP45 unit must be connected to your computer If the MP45 is not properly connected and communicating with your computer you will be unable to record and receive an error prompt Recording a file is beyond the scope of this tutorial other than to practice acquiring a few segments of flatline practice data To acquire useful data electrodes transducers and other devices must be in place to collect signals from your subject If no input devices e g electrodes or transducers are connected to the MP45 acquisition unit but the MP45 is connected to the computer the unit will collect and BSL PRO will display a small flatline value of random signal noise with a mean of about 0 0 Volts If the MP45 unit is connected choose File gt New to open an Untitled graph window and practice recording data If no MP unit is connected read about recording below and proceed to the following section of this tutorial start Start acquisition by clicking the Start button in the lower right corner of the graph window or by pressing Alt Spacebar The circle next to the Start Stop button when green and solid indicates that the MP45 hardware is communicating with the computer ready to record Once an acquisition has started the Start Stop button in the graph window changes to Stop The two opposing arrows to the right of the button indicate that data is being collected The Busy status light on the fro
7. about calibration consult the BSL PRO Manual PRO Tutorial Digital Filtering 21 Note the Digital Filters options of the Input Channel Parameters dialog and read about them at right Saving Input Parameters 22 Read about saving input parameters at right 23 Click Cancel to exit the dialog without changing parameters and return to the Set up Channels dialog Calculation Channel Parameters BIOPAC Systems Inc Digital Fitters Filter 3 n Type Band Stop 60 0000 a 3 000001 Freq Hz The MP45 hardware based Digital Filters are three cascading second order filters that can be set independently using the pull down boxes They are designed primarily for basic signal conditioning e g removing 60 Hz noise rather than for filtering data to retain physiological signals of interest such as retaining alpha activity from an EEG signal Specify a filter type low pass high pass band pass or band stop frequency and Q setting Click Cancel to exit the dialog without changing parameters Choosing OK sets the input channel parameters including any changes you may have made When you save a data file all acquisition parameters for every channel are saved along with the recording This enables you to open a saved data file and collect new data without having to reset any parameters This feature also enables you to save parameters only without data in the Graph Template file format
8. are pasting all the data points of the selected area of the waveform in all channels If you select one second of a wave that was sampled at 200 Hz 200 numbers will be pasted into the Journal The values will be formatted in columns headed by the respective channel names HARE SEC ECG lead 2 FFG Stethoscope Respiration al 2 0 0869751 0 277161 0 119781 0 115596 _ 2 005 O 0694 775 0 256896 0 0761 414 0 112861 2 01 0 0946045 0 296295 0 103607 0 110127 2 015 0 0912476 0 30481 O 0300598 0 107393 2 02 0 0870361 0 313049 0 0326538 0 104563 2 025 O 0663647 0 321472 0 03490625 0 102539 2 03 D 0662427 0 331207 0 0346375 0 100762 2 035 O 06239 75 0 341304 0 03067 02 0 04990527 2 04 0 0697632 0 351013 00 0642395 0 0973438 2 045 Q 0325317 0 360382 0 0698853 0 0956348 2 05 0 0616455 0 368073 0 0648499 0 0936565 27055 EGT Gi 037441 nn7a7 n nainans Journal data may be exported to a file of type txt for subsequent import into other programs such as spreadsheets If you wish to copy graph window data not to the Journal but directly into external programs use the Edit gt Clipboard submenu commands Copy measurements wave data and graphs for pasting directly into word processors spreadsheets and graphic programs Copy Measurement Copy Wave Data Copy Graph PRO Tutorial JOURNAL TEXT TOOLS fo ea ENED 121 Review and practice using the text tools for working with Journal notes N
9. box is crossed over and the display redrawn without this channel Pressing Ctrl and clicking the channel s number box again causes the waveform to reappear www biopac com 14 40 Hold down Ctrl and click again to make it reappear SCALE Adjust Vertical Scale 41 Adjust the Vertical Scale of CHI e Click in the vertical scale region of the channel to generate the Vertical Scale dialog e Change the scale range to one half of its current value e Click OK to set the new scale and close the dialog e Observe the rescaled waveform in the graph window 42 Repeat the previous step to adjust the vertical scale of CH2 CH3 and CH4 43 Select each channel s wavetorm and use the vertical scroll bar to adjust the midpoint of each Adjust Horizontal Scale 44 Adjust the Horizontal Time Scale of all waveforms e Click in the horizontal scale region to generate the Horizontal Scale dialog e Change the scale range to one half of its current value e Click OK to set the new scale and close the dialog e Observe the rescaled waveform in the graph window Biopac Student Lab aaja Ece You can hide multiple channels Hiding an active channel makes the following visible channel become active Note though that a hidden channel can be made active even while hidden by clicking its channel number box Vertical Scale Ragion Major Division mv icdiv Base Point mis m Minor
10. can be useful if you want to print only a portion of the graph Show or hide the waveform channels you wish to print To print a portion of the data file use the zoom tool scale tools and scroll bars to display the portion of the recording you wish to print To print the complete data file choose Display gt Autoscale Horizontal and Display gt Autoscale Waveforms to center waveforms and display the entire recording Print Graph X Printer System Printer Mbac Sales Cancel Print Options Print 4 plots per page Setup Fit to ao pages Print Quality feo0 dpi Copies 1 Print to File Collate Copies P Choosing File gt Print Graph generates a dialog with options that will depend on the printer and operating system you are using Set print options as desired and use the Print Setup command to set paper size orientation etc Consult the BSL PRO Manual for more information about printing Refer to the user manual for your computer and or printer for details about your particular printer www biopac com Biopac Student Lab This completes the BSL PRO 3 7 Tutorial Following is a brief description of other sample files that you may wish to review To further familiarize yourself with features of BSL PRO and see how it can make your work easier open and examine other data sample files included with the BSL PRO installation Sample files include the following DESCRIPTION OF DATA Provides quantitat
11. of data in the display of sample file 4Channel acq 38 Click the CH2 channel number box or its PPG label to the left of the waveform to make it active Hiding channels 39 Hold down the Ctrl key and click on the CH2 PPG channel number box to hide the channel BIOPAC Systems Inc 13 The BSL PRO graph window is designed to provide you with a powerful yet easy to use interface for viewing and manipulating data File Name EET eae ee xj Menu Bar C Fie Edt Transform Display Window MPI5 Help a xj Toolbar eles HUE Id G mean k 9 max poseak Gc deter 27 35200 sec Channel Boxes oga Force Append Markers Marker Label Event Markers increasing gre strength lef hand 11 01 52 AM You can enter marker label fect here Ki EA 20 00 aN m n ra i I I Channels al f L l Horizontal Time 000 l l 64 00 60 00 96 00 fetonds Scale and Scroll Bar af Jae DEHA You can type test into the Journal 2 For example This is Force and EMG data from dominant arm Journal You can also paste wave data and measurements File name Nicholas Joumal Tools Tue Dec 23 2003 zil Status Bar The Biopac Student Lab PRO Display Channel Measurement Result Vertical Amplitude Scales and Scroll Bar Selection Tools Measurement Boxes Marker Tools Selected Area Data or Graph Window Arnow Beam Zoom Status Indicator Journal Window The 4Chan
12. on the MP45 menu and read about some of them below Show Input Values opens a window that displays input data in numerical format as it is being acquired This function is enabled only when the Show Input Values option for a channel is enabled in the Set up Channels dialog Output Control generates a submenu of Output Controls The MP45 outputs signals via ports on its back panel To output analog signals use the Analog Out port to output digital signals use the I O port www biopac com 12 Part 2 Recording 34 Read about recording a data file aD at right If the MP45 unit is not connected read about recording at right and proceed to the following section If the MP45 data acquisition unit is connected practice recording a new data file Choose File gt New to open an Untitled graph window and practice recording Use the Start Stop button in the graph window to acquire multiple short segments of flatline practice data Use the Rewind button in the Toolbar to delete a data segment Choose File gt Close to close the practice graph without saving Biopac Student Lab e The MP45 output function is CH1 to Output For additional output functions including Digital Outputs Pulses Voltage Stimulator BSLSTM and Stimulator SS58L use a BSL MP3X unit Once you have set up the input channels and set up the acquisition parameters you are ready to record To acquire data the
13. Division Of All Channels Precision fi ka digits All Channels coca The vertical scale at the right edge of the graph window displays the amplitude units and range for each channel You can independently adjust the vertical scale of each waveform Click the mouse in the vertical scale region corresponding to that waveform s channel to generate a vertical scale dialog Type in scale values that result in a scale range about half the current value and click OK The waveform s screen amplitude should now be about twice as large as it was before e Note When grids are locked the scale dialog includes grid settings When grids are not locked the dialog includes scale settings only Refer to the discussion of Grid Options later in this tutorial After changing the vertical scale use the vertical scroll bar to adjust the midpoint of each channel The scroll bar acts independently upon each channel you must select the channel first Horizontal Scale LOCKED x Scale Range seconds seconds E m Grid Major Division 0 0 1 0 seconds div foo seconds Minor Division ON N Precision li digits Base Point Honzontal E pace l Hold relative position Region PRO Tutorial The horizontal scale affects all channels 45 Use the horizontal scroll bar to scroll to the beginning and end of the recording AUTOSCALE 46 47 48 49 Autoscale Wavefo
14. EZ StandardCurveData acq Ez EmgwForce acq kz ValidateMeasurements acg Options The sample data file 4Channel acq is used in this tutorial This data file shows simultaneous recordings of ECG Lead II Pulse Pressure PPG Heart Sounds Stethoscope and Respiration This setup provides quantitative data on how the cardiac and respiratory cycles change as metabolic demands on the body change You may also review other sample files to familiarize yourself with BSL PRO applications and tools BIOPAC Systems Inc www biopac com Observe the BSL PRO display and if needed adjust the window so your display resembles the figure at the right SAVING A DATA FILE 9 Save a copy of the sample file if desired To save a copy choose File gt Save As and rename the file 1 e 4Channel_Test acq Biopac Student Lab Biopac Student Lab PRO C Program Files Biopac Student Labi BSL 3 7 65L PRO 3 7 Samples 4C ioj x File Edit Transform Display Window MP35 Help E x Eme EEE C C eeta j CAC eem dee BIEC ee dee ier ee Stethoscope Deep Breath 4 Hebel E OO 1 6 a2 4 5 6 4 6 0 9 6 We 12 6 14 4 16 0 17 6 ee 20 6 22 4 a Seconds 4 Ci Jc aS You can ENTER TEXT and SAVE TEXT inthis Journal so others will see it when they open the file This is a great way to create your own lessons This data file shows simultaneous recordings of 1 ECG Lead I electrode leads
15. Gx BIOPAC Systems Inc Registered to ISO 9001 2000 BSL PRO 3 7 5 BSL PRO TUTORIAL Windows XP or Vista BIOPAC Systems Inc 42 Aero Camino Goleta CA 93117 805 685 0066 Fax 805 685 0067 info biopac com www biopac com 06 02 2009 Biopac Student Lab To learn how the Biopac Student Lab PRO works complete this interactive Tutorial and read the Overview Chapter of the BSL PRO Manual For an in depth discussion of BSL PRO features and how they can make your work easier read further chapters of the BSL PRO Manual The BSL PRO Manual PDF format is under the Help menu of the BSL PRO application The particulars of setup and recording are application specific and are discussed only generally in this Tutorial For detailed instructions about setup and recording consult the BSL PRO Manual and the BSL PRO Hardware Guide and follow your particular lesson plans and application notes This tutorial demonstrates the use of BSL PRO 3 7 5 software with the BIOPAC MP45 data acquisition unit All life science applications for the BSL PRO system involve setting up the hardware for acquiring signals such as electrodes leads and the BIOPAC MP data acquisition unit setting up BSL PRO software acquiring data recording and analyzing the data The purpose of this Tutorial is to familiarize you with the BSL PRO software tools that assist in acquiring and analyzing your data This tutorial illustrates some of th
16. T ENTRY 104 Place the cursor at the beginning of the Journal and type your notes JOURNAL TIME AND DATE TOOLS Time Date AutoTime 105 Enter a time stamp e Place the cursor at the end of existing journal entries Click the Time tool clock icon to enter a time stamp e Review the Journal 106 Enter a date stamp e Place the cursor at the end of existing journal entries a Click the Date tool calendar icon to enter a date stamp e Review the Journal 107 Activate the AutoTime tool and enter time stamps ie G Click the icon to activate AutoTime e Place the cursor where you want the time stamp to be inserted e Press the Enter key e Press the Enter key again e Review the Journal e Click the icon again to deactivate AutoTime PASTE MEASUREMENTS TO THE JOURNAL Set Journal Preferences 108 Choose the menu command File gt Preferences gt Journal to generate the Journal Preferences dialog Biopac Student Lab Type any notes you want directly in the Journal Your keystrokes are entered where the cursor is placed The Journal accepts standard keyboard text entries except certain keystroke combinations reserved for BSL PRO functions Time Date and AutoTime tools are in the top left corner of the Journal window They record the time and or date directly into the Journal e Time and date are entered according to your computer s system clock and calendar If incorrect c
17. The horizontal time scale along the bottom of the graph window denotes when the data was recorded relative to the beginning of the acquisition Autoscale commands determine the optimal scale for waveforms and center their display Note Once waveforms are optimally scaled repeating an autoscale command has no apparent effect As needed to practice autoscale commands individually adjust the scale of each channel as you have done in prior steps and scroll midpoints away from center mi oJ G lead 2 mv Er 4 To optimize the vertical scale of an individual channel select the channel and click the Vertical Autoscale icon in the Toolbar at the top of the graph e Shortcut right click to select a waveform and choose Autoscale from the pop up menu To optimize the vertical scale for all channels and center the waveforms vertically choose the menu command Display gt Autoscale Waveforms www biopac com 16 Autoscale Horizontal 50 Choose Display gt Autoscale Biopac Student Lab Horizontal to optimize the horizontal time scale of all waveforms TERET FFFTF F T TE EAF A FE A F R a A T FTE F A F Y F 51 Choose Display gt Autoscale Waveforms Vertical again displayed l 104 Choosing Autoscale seconds Horizontal and then Autoscale Note the graph display of all waveforms before and after autoscaling Waveforms from the Display menu is the standard way to quickly and easily display the entire record
18. amine any possible reaction Marker labels help reference these data locations The marker that is active selected is colored and its label displayed Append Markers Append markers are displayed in the marker region above the marker label box They are automatically inserted by BSL PRO 3 7 to mark the beginning of recording segments Event markers are displayed below the marker label box They are Event Markers l l manually inserted during or after recording BIOPAC Systems Inc www biopac com 18 57 58 59 60 Marker Tools Marker Preferences Toggle the Markers icon on the Toolbar to hide and show the marker region of the graph window Add and label a new event marker in the event marker region neh Use the Marker Tools to move through the markers and activate the event marker that you added in the previous step eh Click the Marker Tools down arrow to generate a pull down menu Choose Clear gt Active Event Marker to delete the marker that you activated in the previous step Biopac Student Lab T To insert an event marker during recording press the F9 Win or Esc Mac key and enter a label in the marker label text box This enters a marker at exactly the time the key is pressed To insert an event marker after recording click with the Arrow tool in the event marker region above the data point to be marked and enter a label in the marker label text box I Marker Tools
19. are located at the right of the marker region Use the tools to jump through the markers or generate a menu that allows you to search for specific markers to manage markers to paste a summary of markers to the Journal and to generate a dialog to set Marker Preferences The Markers icon on the Toolbar activates the marker display region near the top of the graph window Alternately you may choose the menu command Display gt Show gt Markers Note Not all sample files included with BSL PRO include append markers but all files you create with the software will include automatic append markers at the beginning of each recorded segment Consult the BSL PRO Manual for more information about markers To add a new event marker click with the Arrow tool above the data point you wish to mark in the space between the bottom of the marker label box and the top of the graph My Test Marker Enter a new marker s label by typing in its activated label text box You may change an active marker s label by entering new text in its label box Clicking on an existing marker with the Arrow tool selects that marker and makes it active Use the Marker Tools right and left arrows to move through the markers Move to the marker you added in the previous step When active selected it will be colored and its label displayed The marker that you added is a yellow colored inverted triangle as it 1s an event marker that you added to the record
20. asted into the Journal In the Analysis and Journal sections of this Tutorial you will learn more about taking measurements and pasting measurement data to the Journal Biopac Student Lab To hide channels hold the Ctrl key and click their channel number boxes e Hiding a channel removes its waveform from the graph display e A hidden channel can remain the active channel In the following exercise we will observe the results in the three measurement boxes that are configured for CH3 p p CH4 delta T and CH4 p p If all the measurement boxes are not displayed drag the window wider To practice taking measurements select and compare eight seconds of Deep Breathing data with eight seconds of After Exercise data BIL pe less mv 8 00 sec C ee 039 Liters Stethoscope A E O E y E 12 5 Seconds Labeled markers identify the data segments for each condition When selecting an area of each segment with the I Beam tool note the CH4 delta T measurement to determine 8 seconds of data See env wets Yo cee p dossiers stethoscope aterExercise sd Bra e Seconds Compare the Peak to Peak measurement results for CH3 Stethoscope Heart Sounds and for CH4 Respiration under each condition The menu command Edit gt Journal gt Paste Measurement pastes each measurement to the Journal Review the Journal entries shown below If the Journal is not displayed toggle the Journal icon in the Toolbar So
21. asure CH1 BPM the third CH3 Peak to Peak the fourth CH4 A Time the fifth CH4 Peak to Peak If all five measurement boxes are not displayed drag the window wider The measurements adjust themselves continuously as you scroll through the waveforms with the I Beam tool reflecting the area of data highlighted in the graph window It is important to note that the I Beam always selects either a single point or an area spanning multiple sample points When a single point is selected the cursor will blink When multiple points are selected the area will be highlighted If an area is defined and a single point measurement is selected such as X axis T Time the measurement will reflect the last selected point www biopac com 22 76 Hide CH1 and CH2 ee Compare the p p measurements for CH3 Stethoscope and CH4 Respiration during the Deep Breathing and After Exercise segments of the recording e Using the I Beam tool select 8 seconds of data in the Deep Breath segment Note the p p measurement results in the measurement boxes for CH3 and CH4 Choose Edit gt Journal gt Paste Measurement Read the measurement results that have been pasted into the Journal e Select 8 seconds of data in the After Exercise segment Note the p p measurement results in the measurement boxes for CH3 and CH4 Choose Edit gt Journal gt Paste Measurement Read the measurement results that have been p
22. ate as a simple threshold Tihs detector or can include more sophisticated pin 40 0000 BPM parameters such as noise rejection and Mons 160 0000 BPM windowing a FF Find rate of entire wave BSL PRO calculates the sii threshold values and computes the rate for the entire waveform The rate is charted and added to the current graph window Options enable you to limit the transformation to a selected area and to display thg rate graph in a new window To remove a channel from a file select it and choose Edit gt Remove Waveform Take care to select the channel you wish to remove as you may not undo this command A related type of transformation is the histogram which allows you to display data in summary format and examine the central tendency characteristics and variability within a waveform www biopac com 26 93 94 95 96 vee 98 Select CH1 ECG Choose Transform gt Histogram Set the bins value to 50 Click OK Close the Histogram graph window without saving Show all four channels of the 4channel acq graph DISPLAY MODES 99 100 ISIE e Click on the Scope display mode icon e Choose Display gt Tile Waveforms e Choose Display gt Overlap Waveforms e Choose Display gt Autoscale Waveforms e Hide CH1 and CH2 e Analyze the CH3 and CH4 waveforms lel Click on the X Y Plot display mode icon e Set the X axis to Respiration Biopac Studen
23. b You may use the Arrow tool to adjust chart boundaries and better view a waveform s track Position the Arrow tool over a boundary line between waveforms When the cursor changes style drag to resize the track of that waveform The I Beam tool similarly changes style and can be used to adjust chart boundaries EA Pi Ls PPG Choose Display gt Reset Chart Display to evenly map display the waveform tracks in the graph window spirat ja The I Beam tool is used to select an area of a waveform or multiple waveforms to be edited measured or transformed Clicking and dragging the I Beam tool across waveforms selects and highlights an area of data You can perform a variety of operations editing measuring transforming copying pasting measurements to the Journal on the selected data As you scroll though waveforms with the I Beam tool and highlight areas of data to be measured note that the values in the channel measurement boxes above the graph window adjust themselves continuously It is important to note even without dragging that the I Beam tool always selects at least one sample point If a single point is selected the cursor will blink If multiple points are selected the area will be highlighted em fo Cn e 0 Cae CO Cee HAHH sce Dana ee m A O O G L L 000 320 640 960 1260 1600 1920 22 620 700 Th asb S40 1020 1100 F seconds k I Q k Ha The Zoom tool
24. bottom third of dialog Set the first cursor to Peak 45 sec and the second cursor to Peak 45 sec 83 Click the Ave Start button 84 Close the Averaging result window without saving and return to the graph display window THE TRANSFORM MENU 85 Scroll through the options under the Transform menu command and note the available functions Biopac Student Lab x Source Channel ECG lead 2 Find peak 0 Positive peak Negative peak User defined interval Threshold Level O far7reort mv Tracking Start point f Cursor C Start time 0 000000000 SEC Interval rat cursor to Min interval 0 005000000 Set second cursor to Peak 0 45 36 A Paste measurements into journal O Cmo Awe Start Don t Find Note the settings in the Find Peak dialog BSL PRO automatically calculates a threshold level based upon a percentage of the peak in the selection of data that you highlighted Control Channel cH 1 ECG lead 2 Setup Averaging esen Choose Off line Averaging and set the first and second cursors relative to the peak B Averaging Sgi BSL PRO runs through the data and z generates a new window showing the ON ge average ECG for the entire recording You may also set Find Peak to average a selected portion of the recording This feature is useful when comparing the ECG complex during different sections o
25. e basic features of the Biopac Student Lab PRO software and assumes you have already installed BSL PRO to your hard drive If not insert the CD and follow the Install Wizard You do not need to record data nor be connected to the MP recording hardware to conduct this tutorial You do not need to be connected to MP recording hardware to analyze any data that you yourself may record and save to disk This allows you to review a previously saved file and analyze it with the powerful BSL PRO software on any computer at home or in the lab even when that computer is not connected to the MP data acquisition unit Biopac Student Lab 3 7 5 Choose a file to open Look in Oo Samples p E 4Channe l acq Z BloodPressure acq earthworm data acg earthworm smooth muscde acg EmgwForce acq FingerTwitch 1 acq FingerTwitch2 acq NerveConduction acg StandardCurveData acg ValidateMeasurements acg be k fz E E E E Ez Ez File name 4Channel acg Files of type esL PRO files aca ig PRO Tutorial 3 Se OS C i BIOPAC Systems Inc 9 Biopac Student Lab 3 7 5 Less With default installation sample data files are located Main Drive Program Files BIOPAC Systems Inc Biopac Student Lab 3 7 5 Lessons amp PRO Sample Data PRO open j zx EZ FingerTwitch 1 acq EZ FingerTwitch2 acq EZ NerveConduction acq EZ earthworm smooth musde acq StandardCurve acg 2 EEG acq
26. ect a portion of a waveform to edit or analyze Once you have selected highlighted an area you can perform a variety of operations such as editing measuring or transforming data D The Arrow tool is a general purpose cursor that is used to select a waveform or channel scroll through data adjust chart boundaries between waveforms select options from pull down menus etc All other cursors default to the arrow style when moved outside of the waveform region www biopac com 20 64 65 Use the Arrow tool to resize the chart boundaries between waveforms Choose Display gt Reset Chart Display to return the display to equal sized waveform tracks I Beam Tool 66 67 68 Select the I Beam tool in the lower right corner of the graph window Click and drag the I Beam tool across the waveforms to select an area of data Click the I Beam tool in the wavetorms without dragging to select a single data point Zoom Tool 69 70 71 ee Ta Select the Zoom Tool in the lower right corner of the graph window Click drag and release the Zoom tool in a waveform to select and magnify a section of data Zoom again Choose Display gt Zoom Back or press Ctrl Control Minus keys to undo the previous Zoom command Choose Display gt Autoscale Horizontal and Display gt Autoscale Waveforms to center all waveforms and display the entire recording Biopac Student La
27. ed file My Test Marker He ll es Find Active Event Marker cer o The Marker Tools down arrow generates a menu and submenus of marker commands Use the Clear gt Active Event Marker command to clear the event marker that you added and activated in previous steps Take care that you have activated the marker you wish to clear as you may not undo this command PRO Tutorial 61 Le Click the Marker z Tools down arrow and choose Preferences to generate the Marker Preferences dialog 62 Click through the tabs of the Marker Preferences dialog to note the options available for configuring custom marker labels and for pasting a marker summary to the Journal SELECTION TOOLS RPT Q Arrow Tool 63 Select the Arrow tool in the lower right corner of the graph window BIOPAC Systems Inc Marker Preferences Event Markers Sequential Joumal Summary Append Markers Event Markers Fined NS Clear b Find For Marker Label Use Default text Segment 1 Segment 2 etc Summary to Journal f Use the following custom labels Show Label Forearm 2 Increasing Clench Force Add To List egment H Click the Marker Tools Label Forearm 1 Wlas Force down arrow and choose 2 Forearm 1 Increasing Clench Force 3 Forearm 2 Mas Force Preferences to generate a Marker Preferences dialog Alternately choose File gt __ Rename Delete Delete all Preferenc
28. es gt Markers IM Include a system time stamp i e 10 44 46 AM Marker Preferences has Pence fon three tabs of options to auto label markers and one to format marker data summarized to the Journal op Battom Marker Preferences has tabs that allow you to pre establish custom labels before recording that are entered when a marker is inserted during recording Custom labels rather than the default labels Segment 1 Segment 2 etc can be pre established for the append markers that are automatically inserted at the beginning of each new recording segment Custom labels either fixed or sequential can be pre established for event markers that are inserted with the press of a function key while recording This can help you enter markers with descriptive labels during recording at exactly the moment an event occurs when you otherwise might not have time to label your markers Note also the Preferences tab that sets format and sort options for marker summaries that you may paste to the Journal When working with data you will use one of three selection tools Arrow I Beam and Zoom Selection Tool icons are located in the lower right corner of the display window Click on an icon to activate the tool indicated for the editing or analysis function to be performed Selection tools may also be activated by choosing the menu command Display gt Cursor Style You will often use the Zoom tool and I Beam tool to sel
29. eturn to the Set up Channels dialog T Click the Presets icon of Calculation Channel C1 and choose Off 29 Click the close box to exit the Set up Channels dialog and return to the graph window SET UP ACQUISITION 30 Choose the menu command MP45 gt Set up Acquisition 31 Note the acquisition parameter options of the Set up Acquisition dialog and read about them at right Biopac Student Lab CALCULATION CHANNELS dpfdt Min ae ae al RR interval dpyidt Max p ECG R R Interval ee Amplituckss Pints C3 calculation OFF dae calm tte ota c4 calculation OFF EEG beta 13 30 Hz EEG delta 1 5 Hz EEG theta 4 6 Hz EMS Integrated For example you can set up a calculation channel C1 to compute the average R R Interval of the ECG data on analog input channel CH 1 C2 calculation OFF al To do this click the Presets icon down arrow to the right of Calculation Channel C1 to generate a menu of calculation channel presets Choose the preset option ECG R R Interval SOUrCe CH1 ECG lead 2 jw Remove baseline Iv Auto threshold detect Noise rejection 5 To of peak Wew hannel Preset Scaling Cancel Note the Rate Parameters dialog for the ECG R R Interval preset you have chosen The Source data for the calculation is acquired from CH1 ECG lead 2 and the Function is to compute the interval in seconds Were you to record with these rate parameters Ana
30. ew Journal Load Text Save Text Print Journal Part 6 Printing Graphs 122 Read about the Print functions to the right 123 Adjust the graph window to show the portion of the graphs you wish to print 124 Choose File gt Print to generate the Print Graph dialog 125 Choose OK to print the graph window 126 Review the printed result BIOPAC Systems Inc 31 The Journal is saved with the data file If the data file is closed and reopened the Journal notes and window position will be as when the file was last saved Journal Text Tools are activated by icons in the Journal Toolbar at the top of the Journal and below the graph window m New Journal deletes all text currently in the Journal Load Text enables the import of data from other files into the Journal It generates a window to import files which are limited to files of type txt Save Text allows you to export your Journal notes to a separate file of type txt This is useful for exporting data to other programs such as spreadsheets for further manipulation and analysis Print Journal prints the contents of your Journal The File gt Print command will print what is displayed in the graph window You control how data will be printed by controlling how the data is displayed on the screen For instance if you ve zoomed changed scale or hidden a channel only the portion of data displayed in the graph window will be printed This
31. f an experiment Transform The Transform menu contains a number of Digital Filters functions that modify waveform data Math Functions Template Functions The Digital Filter sub menu has both FIR and Integral IIR types of filter operations For most datasets Derivative the default filter parameters may be used and will M produce relatively robust results Smoothing Diffsrence The Math Functions sub menu lists several Histogram available mathematical transformations Some Resample l S transformations produce a dialog with parameters Waveform Math that can be changed EFT The Template Functions sub menu provides a Find Peak cmp host of options to examine the relationship Find Next Peak Ciri E between two different waveforms Find All Peaks CthitR Find Rate Cris The Integral transformation results in a running total of all selected waveform values using a trapezoidal rule integration PRO Tutorial TRANSFORM gt FIND RATE 86 Select CH1 ECG 87 Choose Transform gt Find Rate 88 Select the function Rate BPM 89 Check to enable Find rate of entire wave 90 Click OK BSL PRO calculates the rate and displays its graph 91 Note the new CH5 Rate channel that has been added to the graph window 92 Remove the new channel selecting CH5 and choosing Edit gt Remove Waveform Transform gt Histogram BIOPAC Systems Inc 25 The Derivative trans
32. formation approximates an ideal differentiator It allows you to specify a low pass frequency to filter the data prior to performing the derivative The Difference transformation is a running subtraction over the number of points specified The Expression option has an Equation Generator that lets you perform a range of mathematical operations from addition and subtraction to arcsine and log transformations You can perform complex operations in a single step For example you can compute the mean of several channels and then use the arcsine function to transform the result and save the output to a new channel You can transform entire waveforms or sections of waveforms In addition to performing mathematical functions the BSL PRO software can search for peaks and calculate rate The Find Rate function will calculate rate information for a variety of cyclical data including BPM Hz peak max peak min P P area and mean For example suppose you want to calculate BPM for the entire ECG waveform once it has been collected To do this select CH1 and choose the menu command Transform gt Find Rate to Euinction x generate the Rate Detector dialog View Peak detect the functions available on the pull down Positive Negative menu and choose Rate BPM FF Remove baseine F Auto threshold detect Noite rejection 5 00 of peak Note the other options allowing you to customize the Rate calculation The Rate Detector can oper
33. heck your system settings Once the cursor is placed in the Journal click the Time tool clock icon The current time is entered at the cursor point di l Once the cursor is placed in the Journal click the Date tool calendar icon The current date is entered at the cursor point 5 Click the AutoTime tool clock icon with arrow upon activating the icon will appear depressed Then place the cursor in the Journal and press the Enter Return key If the AutoTime tool is activated and if the cursor is positioned in the Journal text region the current time is entered each time you press the Enter key e To reset the Enter key to its normal Return function in the text editor toggle the AutoTime icon again The AutoTime function records the time at the instant the Enter Return key is pressed This is very useful for entering time stamps during recording while data is being collected Journal Preferences options control how measurements and wave data are formatted when pasted directly from the graph window to the Journal using the Edit gt Journal submenu commands You can also set general text options for the Journal in Preferences To set the text word wrap option right click anywhere in the journal text entry region PRO Tutorial 109 110 111 Review the options that determine how measurements and wave data are pasted into the Journal Check that all Measurement Paste Options are selected a
34. id Options generates the Grid Options dialog with settings that control the display and locking of grid lines Alternately right click in the waveform region and choose Grid Options Note that the Lock grid lines option can affect the grid display In general Unlocked grids can help you view the data display on the monitor while Locked grids can help with printing See the BSL PRO Manual for more information about grids and grid options When grids are locked the scale dialog options include grid settings When grids are unlocked scale dialogs include scale options only as shown in these two screen shots of the vertical scale dialog Refer to the discussion of Scale earlier inthis PESO ewe Tutor ial Scaling x Channel fi ECG lead 2 Scaling 3 All Channela Scale Major Division my tei All Channels 1 0 Base Point 0 0 m Minor Division OFF Midpoint 0 3 m All Channels wi Precision Precision fi digits All Channels All Channels ho digits All Channels corc men MARKERS 56 Read about markers at right Append Markers Marker Label Event Markers Marker Tools selected Arca Markers are used to identify important data points in a recording so they can be referenced later For instance you may want to note when an experimental condition began or when an external event occurred so you can ex
35. ified signal appears once data is collected Some types of signals such as EEG typically need greater amplification than other types of signals such as ECG or EMG although ideal gain settings are best determined on a case by case basis To set the gain for a given channel choose a value from the pull down menu Higher gain results in greater amplification Setting the gain too high results in data that is clipped Setting the gain too small results in data that appears flatlined For the best resolution establish gain such that the maximum peaks BIOPAC Systems Inc www biopac com Offset Input Coupling Scaling 18 Click the Scaling button to 19 ZY generate the Change Scaling Parameters dialog Note the scaling parameter options and read about scaling and calibration at right Click Cancel to exit the dialog without changing scaling parameters and return to the Input Channel Parameters dialog Biopac Student Lab of the signal are close to the maximum range Consult the BSL PRO Manual for a complete discussion of gain Offset enables you to correct the offset of an incoming analog signal by adding or subtracting a constant prior to amplification The correction is generally zero but may be set if a transducer or electrode has inherent offset a condition especially true of signals collected in DC mode Input coupling is set for the type of signal you are recording For example AC coupli
36. ing seconds To optimize the horizontal scale for all waveforms and make all the data visible in the graph choose the menu command Display gt Autoscale Horizontal This sets the horizontal time scale range to start at 0 beginning of acquisition and end at the end of the recording The entire recording is displayed e Shortcut click on the Horizontal Autoscale icon in the Toolbar at the top of the graph window GRIDS AND GRID OPTIONS ae ae x a Toggle the Grid icon in xl the Toolbar to show grid M Show minor division lines Major grid lines Minor grid lines 53 Right click in the waveform sve Ail region and choose Grid Yicth __ Em Options from the pop up menu a C E to generate the Grid Options mi dialog IW Lock grid lines 5 4 Note the available grid options to adjust the horizontal or vertical scale when grid i locked uze Toggle the option Show minor are locked use division and click OK to view StartEnd parameters changes in the display Middle PointRange parameters Cancel PRO Tutorial 17 Note that the option to Lock grid lines affects grid display 55 Right click in the waveform The Grids icon on the Toolbar shows or hides grids Alternately region to generate a pop up choose the Display gt Show gt Grid or right click in the waveform region and menu and deselect Grid to select or deselect Grid hide the grid lines Display gt Show gt Gr
37. ion OFF c3 TE PT calculation OFF Y 4F TPF PT 4 calculation OFF a i Off New ECG RA Interval ECG R wave Amplitude EEG alpha 8 13 Hz EEG beta 13 30 Hz EEG delta 0 5 4Hz Analog Input Channels are the most common type of channel and are used to acquire any data with continuous values Examples of this include nearly all physiological applications where input devices transducers and electrodes produce a continuous stream of varying data e BSL PRO records and displays up to two analog signals from devices connected to analog input ports on the front of the MP45 acquisition unit Calculation channels transform data in some way rather than collect external data as input channels do Calculation channels acquire data from other channels The original source data is not altered it is mathematically transformed and stored in a modified form in the new calculation channel e For example if you wanted to know the difference between CH1 and CH2 data for every point of data collection you could set up a calculation channel to acquire the data from those two analog channels and plot a waveform of the difference e Up to 12 calculation channels can be configured This allows for complex calculations to be performed that involve two or more calculation channels such as filtering ECG data and then computing BPM www biopac com 6 Channel Specification Options 12 N
38. ion properly under normal use and within the manufacturer s specifications This warranty does not apply if in the sole opinion of BIOPAC Systems Inc your BIOPAC Systems Inc product has been damaged by accident misuse neglect improper packing shipping modification or servicing by other than BIOPAC Systems Inc Any returns should be supported by a Return Mail Authorization RMA number issued by BIOPAC Systems Inc BIOPAC Systems Inc reserves the right to refuse delivery of any shipment containing any shipping carton without the RMA number s displayed on the outside The Buyer shall prepay transportation charges to the site designated by BIOPAC Systems Inc BIOPAC Systems Inc makes no warranty or representation either expressed or implied with respect to this software its quality performance merchantability or fitness for a particular purpose As a result this software is sold as is and you the Buyer are assuming the entire risk as to its quality and performance In no event will BIOPAC Systems Inc be liable for direct indirect special incidental or consequential damages resulting from any defect in the software or its documentation even if advised of the possibility of such damages or for damage of any equipment connected to a BIOPAC Systems Inc product Trademarks Biopac Student Lab is a trademark of BIOPAC Systems Inc Windows is a trademark of Microsoft Corporation Mac OS X is a trademark of Apple Com
39. ive data on how the cardiac and respiratory cycles change as metabolic demands on the body change Demonstrated in this Tutorial Shows simultaneous recordings of Blood Pressure and Korotkoff Sounds Shows the nerve response from an isolated earthworm nerve Shows spontaneous contractions from isolated earthworm gut smooth muscle and the peak to peak response of each contraction Shows how EEG activity changed when the Subject opened and closed her eyes markers indicate change Shows simultaneous recordings of Clench Force and EMG correlating motor unit recruitment to increased power of skeletal muscle contraction and measuring changes in EMG and Force when the muscles become fatigued Shows simultaneous recordings of Force Displacement and Stimulator output The Stimulator pulse induced skeletal muscle twitch causing the Subject s finger to lift a small weight Channel displays the force generated from the twitch of a finger after a stimulus was delivered to the forearm of a human subject Channel 2 shows the stimulus The event markers above the graph window mark the points in the recording when the stimulus frequency was increased Channel 1 displays the stimulus voltage and Channel 2 the motor EMG response of a muscle The display is in the Overlap Segment mode which is an easy way to view the EMG response to different levels of stimulus Shows a typical Standard Curve used in colorimetric studies to find the concentration
40. log Input Channel CH1 would acquire the ECG data from the subject and Calculation Channel C1 would in turn compute the R R Interval of the data collected on CH1 Choosing the Off preset option turns off the calculation channel It will no longer acquire plot nor display data Once you have set up the channels to be acquired the next step is to set up the acquisition parameters The Set up Acquisition dialog displays options that control among other things how and where data will be stored the sample rate for data collection and the duration length of each acquisition Default acquisition parameters are to Record and Append using PC Memory at a sample rate of 200 samples per second for 30 seconds PRO Tutorial Data Storage Options Sample Rate Acquisition Length Repeat 32 Click the close box to exit the Set up Acquisition dialog and return to the graph window OUTPUT CONTROL AND OTHER MP45 MENU OPTIONS 33 Click on the MP45 menu and view other available commands BIOPAC Systems Inc Set up Acquisition x Record and Append using Pc Memory Sample Rate 200 0 Samples second Reset Max acquisition length Current acquisition requires Acquisition Length al Repeat every 0 f times The three pull down menus at the top of the Setup Acquisition dialog allow control over how and where the acquisition data will be saved Default parameters are set to Record and Ap
41. magnifies a portion of any wave for examination Click on the waveform with the Zoom tool in the area you wish to examine or hold the left mouse button and drag the Zoom tool so it forms a box over the desired area Then release the mouse button to display the enlarged area The Zoom tool magnifies all waveforms You may want hide other channels to focus on the channel you wish to zoom in on You may undo previous zooms up to 5 by choosing Display gt Zoom Back or by pressing Ctrl Control Minus keys Choosing Autoscale Horizontal and then Autoscale Waveforms Vertical from the Display menu is the standard way to quickly and easily return to a display of the entire recording PRO Tutorial MEASUREMENTS Measurement Boxes 74 Read about measurement boxes at the right Taking Measurements 75 Note the measurement boxes in the 4channel acq sample file As you drag the I Beam tool across waveforms note the change in values in the channel measurement boxes BIOPAC Systems Inc 21 Using the BSL PRO measurement tools you can easily use absolute functions such as value X axis time and samples that measure a single point and use functions such as min max mean and A Time that operate over a Selected highlighted area of data Measurement features can be automated so that measurements are taken and pasted into the Journal file when a specific event occurs or at pre specified user defi
42. ned time intervals Functions under the Transform menu such as Find Peak identify specific events based on a variety of threshold and window discriminators ee m 4 Time 117 400 sec SC delta 0 02960 5 mean 141 32612 sc none TAHE SC Selected Channel none Ch1 ECG lead 1 value Ch2 ECG lead 2 delta Ch4 Respiration s Pull down 3 PP Ch5 Arterial blood pressure max channel selection ee box mean stddev Pull down rece measurement box rae The measurement region is located near the top of the graph window For each measurement there are pull down boxes that specify the channel to be measured and type of measurement and a value box that displays the results To specify the channel to be measured click on the channel selection box and choose from the pull down menu When the designation SC is chosen default measurements are taken from the channel that is active or selected in the graph window To specify a measurement type click on the measurement box and choose from the pull down menu Measurement results are displayed in the box to the right of the measurement type Results reflect the waveform data selected in the graph window with the I Beam tool apo epee eee sev ate sec setters The sample file 4Channel acq displays five measurement boxes in the region below the Toolbar The first is configured to measure CH1 delta the second to me
43. nel acg sample file contains four different types of data each in its own channel with a border between each waveform display To the left of each waveform is a channel label color coded to help identify each waveform ECG Lead 2 PPG Pulse Pressure Stethoscope Heart Sounds Respiration PPG ECG lead 2 In the upper left of the graph window a row of small numbered boxes indicate the acquired channels The color for each channel s waveform label and indicator box correspond and can be changed The box on the m mi D i a te i T p ce a a iz left corresponds to the waveform at the top of the screen The box that appears depressed is the selected or active channel The label of the active channel is displayed to the right of the channel number boxes and is highlighted in the graph window to the left of the waveforms To make a channel active select its channel number box or its label with the arrow tool Only one channel can be active at a time In the example shown here Channel 2 PPG is active Transformations and editing operations generally apply to the active channel though in some cases to multiple channels Measurements can be taken from any channel whether active or not To focus on one channel it is sometimes helpful to temporarily hide another To hide a channel click on that channel s number box while holding down the Ctrl Control key The channel s number
44. ng is generally used for biopotential signals such as ECG and EEG while DC coupling is best for transducer signals measuring absolute values such as force pressure and temperature In AC coupled mode you may adjust the upper limit of the bandwidth of the signal being recorded by choosing one of three high pass HP filter options The Scaling button at the bottom of the Input Channel Parameters dialog generates the Change Scaling Parameters dialog which allows conversion of incoming signals into other units such as ft Ibs millimeters liters etc Change Scaling Parameters xX CH2 PPG 5 35 Hz Input value scale value Cali ja 000 microV Calz 1000 micro 1 Units label mi Scaling allows you to easily translate the voltage read by the MP45 into the units being measured Note that for CH2 PPG the Units label is mV To change the label enter text and click OK for instance type millivolts Calibration is performed prior to acquisition never while recording Input values Call and Cal2 from a transducer may be calibrated to known low and high values 1 e temperature When an acquisition is performed data samples from the selected channel are scaled accordingly and the vertical amplitude scale reflects the rescaled units Calibration is unique to each application and not demonstrated in this Tutorial To calibrate follow the guidelines of the specific lab lesson or application note To read more
45. nt of the MP45 also indicates that data is being collected stop 1 at pe Stop an acquisition at any time by clicking the Stop button or by pressing Alt Spacebar An acquisition automatically stops when it reaches the Acquisition Length parameter in the Set up Acquisition dialog In the default Append mode BSL PRO can record multiple segments in a single file Simply Start again to append another recording segment An append marker indicates the beginning of each new recording segment KI The Rewind button on the Toolbar deletes the last recorded segment You cannot change acquisition parameters while recording If you change acquisition parameters during a pause between recording segments when you attempt to start recording again BSL will warn that your previous data will be overwritten unless the Warn on Overwrite option in the MP45 menu is disabled Similarly deleting a recorded segment with the Rewind command will generate a warning After recording multiple short segments choose File gt Close to close the practice data window without saving Recording is unique to each application Follow the recording guidelines for your specific lab lesson experiment or application note To read more about recording consult the BSL PRO Manual PRO Tutorial Part 3 Display 36 Note the features of the BSL PRO display in the labeled figure at right CHANNELS Selecting Channels 37 Note the four channels
46. orms are in a single window with no borders between channels Waveforms can overlap Display menu options determine the display of waveform data Analyze the relationship between CH3 Stethoscope Heart Sounds and CH4 Respiration Note the differences between the Deep Breath and After Exercise data segments X Y Plots are useful for respiration studies vectorcardiograms and investigations into non linear dynamics In X Y display mode the X axis and Y axis labels correspond with the channels that are being plotted To set the axes click on the labels and choose PRO Tutorial e Set the Y axis to Stethoscope 101 Return to the Chart display mode show all channels and autoscale waveforms Part 5 Journal 102 Toggle the Journal icon on the Toolbar to hide and show the Journal region of the display 103 Adjust the size of the Journal region by dragging the boundary that separates the Journal from the graph window BIOPAC Systems Inc 27 the channel to plot from the pull down menu In X Y display mode the I Beam tool becomes a cross hair When scrolled across the graph window the X axis and Y axis values are displayed in the measurement region above the graph window In X Y display mode the icon for the Plot Standard Curve function 1s enabled in the Toolbar it generates a dialog that enables you to enter values for colorimetric studies Click on the Chart display icon to return
47. ote the three checkbox settings for each channel that determine whether a channel acquires data whether the data is plotted as a waveform on the screen and how the data values themselves may be optionally displayed Acquire Data Plot on Screen Enable Value Display Channel Presets 13 _ Click the Presets icon down arrow to the right of any analog channel label to view a menu of parameter presets Press ESC Escape key to close the menu without selecting a new preset option 14 Biopac Student Lab B Set up Channels l x Channel Acquire Data Label Presets View flhange Plot on Screen Parameters Enable Value Display ANALOG INPUT CHANNELS CHI M ei JECG 5 35Hz CH2 wie fe PPG 5 35Hz CHS fw fe fe Stethoscope Heart Sounds CH4 w fv fw Respiration _ _ _ _ m _ _ _ Acquire Data records data into memory via the selected channel but the data will not be displayed unless you also select Plot on Screen Plot on Screen displays the recorded data on screen so you can view the waveforms e In some cases you may not want to plot data on the screen For example you may want to acquire data on an input channel and run a calculation function before displaying it on screen In that case you would select Acquire Data but not Plot on Screen for the Input Channel and Acquire Data and Plot on Screen for the Calculation Channel Enable Val
48. pend using PC Memory which tells BSL PRO to append acquisitions until the file is saved and to store the data in computer memory RAM during the acquisition Options can be set to save data once each segment is acquired to autosave and to utilize the computer s hard disk for storage Sample Rate determines how many samples the MP45 will acquire per second and is analogous to the mm sec setting on a chart recorder Choose the desired value in the pull down menu you may need to scroll up to see higher values The default sample rate is 200 samples per second but the best sample rate will vary depending upon the nature of the data being acquired Acquisition Length is set using the scroll bar or entering the length directly into the value box Units are set in the pull down menu to the right Length of acquisition will vary depending upon the experimental design The MP45 will stop acquiring data when the specified length is reached You may also stop acquisition at any time by clicking on the Stop button that is enabled during recording in the lower right corner of the graph window Acquistion Length 30 OO0000 seconds 4 samples MSEC seconds Repeat every 0 minutes hours The Repeat option allows you to acquire data from repeated trials using the Same parameters for each trial second il tines Consult the BSL PRO manual for more information about acquisition parameters Note the options
49. puter Inc This document was created with Microsoft Word for Windows Adobe Photoshop Adobe Distiller Corel Draw 7 0 Mainstay Capture and JASC Inc JasCapture BIOPAC Systems Inc www biopac com
50. rms Vertical Select a channel Click the Vertical Autoscale icon in the Toolbar at the top of the graph window to optimize the vertical scale of the selected waveform Right click in another channel to select it and choose Autoscale from the pop up menu to optimize the vertical scale of that selected waveform Choose Display gt Autoscale Waveforms to optimize the vertical scale of all waveforms BIOPAC Systems Inc 15 You can adjust the horizontal scale to any range to compress or expand the displayed waveforms along the horizontal time axis The horizontal scale affects all channels Click in the horizontal scale region to generate the horizontal scale dialog Type in an upper range value that is about half the current value and click OK The horizontal scale of all waveforms should now be about twice as large as it was before e Note When grids are locked the scale dialog includes grid settings When grids are not locked the dialog includes scale setting only Refer to the discussion of Grid Options later in this tutorial Adjusting horizontal scale allows you to magnify the screen display to better examine a waveform but note that the waveforms may no longer fit in the data window The file however contains the complete record even if all data is not displayed on the screen To view the beginning of the recording time zero scroll left with the horizontal scroll bar To view the end scroll right
51. s well as the Include time values option of the Wave Data Paste Options Click OK to accept any preference option changes Set Measurements EA es 114 Set a measurement box for CH1 BPM e Choose CH1 as the selected channel e Choose bpm as the measurement Set a second measurement box for CH1 delta T Time e Choose CH1 as the selected channel e Choose delta T as the measurement Use the I beam tool to select an area of data from the peak of one R wave to the peak of the next R wave BIOPAC Systems Inc For this tutorial select all of the measurement and wave data paste options so when you paste data to the Journal you can easily identify them When you plan to export measurements and data to other programs such as an external spreadsheet program it is sometimes best not to select all of the options as some may affect formatting 29 x f O0 inch Change Fort Measurements Paste Options Tabs M Include measurement name M Include measurement units M Include channel numbers M Put each measurement on a separate line Wave Data Paste Options i Include time values Cancel From the pull down menus in the measurement box choose CH1 as the selected channel and BPM as the measurement e TIP If the channel you wish to measure is the active channel you can set SC selected channel as the channel to be measured From
52. s of unknown solutions Demonstrates multiple measurements on a selected area of data PRO Tutorial 33 Copyright Information in this document is subject to change without notice and does not represent a commitment on the part of BIOPAC Systems Inc This tutorial and the software it describes are copyrighted with all rights reserved Under copyright laws this tutorial or the software may not be copied in whole or part without the written consent of BIOPAC Systems Inc except in the normal use of the software or to make a backup copy The same proprietary and copyright notices must be affixed to any permitted copies as were affixed to the original This exception does not allow copies to be made for others whether or not sold but all of the material purchased with all backup copies may be sold given or loaned to another person Under the law copying includes translating into another language or format Biopac Student Lab Tutorial including all text and graphics are 1998 2009 BIOPAC Systems Inc with all rights reserved Warranty BIOPAC Systems Inc warrants its hardware products against defects in materials and workmanship for a period of 12 months from the date of purchase If BIOPAC Systems Inc receives notice of such defects during the warranty period it will at its option repair or replace the hardware products that prove to be defective This warranty applies only if your BIOPAC Systems Inc product fails to funct
53. t Lab Choosing Transform gt Histogram generates the Histogram Options feo pe dialog The dialog prompts you to enter fe Autorange the number of bins to sort the values into and if you do not accept the default autorange to enter the upper and lower bounds of the data to be sorted Type 50 in the bins entry and click OK The resulting waveform is generated in a new graph window and should resemble the histogram plot shown here Return to the 4Channel acq graph window Show channels that are hidden by holding the Ctrl key and clicking their channel number boxes BSL PRO offers a number of display options including Scope Chart X Y Plot and Overlap Segments Chart is the default display mode You can switch from one display mode to another using the Toolbar icons in the upper left hand corner of the window By clicking on these icons you can alternately have your display emulate a chart recorder Chart mode oscilloscope Scope mode or plot data from one channel against data from another channel X Y Plot Mode Overlap Segments display is useful for comparing the waveforms of appended segments The icon for this mode is enabled only in data files with multiple appended recording segments Overlap Segments mode is not enabled in the sample files included with BSL 3 7 Consult the BSL PRO manual for more information about display modes The Scope display mode emulates an oscilloscope All wavef
54. the pull down menus in another measurement box choose CH1 as the selected channel and delta T Time as the measurement ECG lead 2 Deep Breath gt IB Zoom in on the CH1 ECG data Identify and select a region from the peak of one R wave to the peak of the next R wave www biopac com 30 Paste Measurement Data 115 Click the cursor in the Journal where you want to paste the measurement 116 Choose the menu command Edit gt Journal gt Paste Measurement 117 Review the Journal entry PASTE WAVE DATA TO THE JOURNAL 118 Use the I Beam tool to select the portion of the waveform you are interested in 119 Click the cursor in the Journal where you want to paste the wave data and choose Edit gt Journal gt Paste Wave Data 120 Review the Journal entry Biopac Student Lab When you use the Paste E Measurement function olf all the measurements showing a value will be written to the Journal If you do not want a particular measurement pasted to the Journal go to the pull down menu for that measurement and select the option none Duplicate Waveform Select All Remove Vravreform Clipboard Jounal H Paste Measurement Cirl M Paste Wave Data CirleD The Paste Wave Data function writes to the Journal all the data points of the selected area in the graph window It is very easy to put a lot of data into the Journal using this command You are not pasting simply a measurement you
55. to Chart mode Show any hidden channels by holding the Ctrl key and pressing their channel number boxes Choose Autoscale Horizontal then Autoscale Waveforms to optimally scale and center all waveforms File Edit Transform Display Window MP35 Help Seid Edi ILS EA E eee p z Be A Show jride journal ECG lead 2 0 0 ace 6 4 9 6 12 5 Seconds p Slaa EE You can ENTER TEXT and SAVE TEST in this Journal I so others will see it when they open the file This is a great way to create your own lessans This data file shows simultaneous recordings of 1 ECG Lead ll electrode leads BIOF AC SS2LA Pulse pressure PPG BIOPAC ss4Lai 3 Heart Sounds Stethoscope BIOPAC SS30L 4 Respiration RSP BIOPAC S35L Ready Grid Off Chart Memory The Journal is a text editor built into the BSL software that allows you to record notes and data at the same time you are acquiring and analyzing data Journal entries are saved with the BSL PRO data file You can copy measurements and wave data in numeric format from the graph to the Journal and export Journal data to other programs for further analysis Toggle the Journal icon on the Toolbar to display the Journal region Alternately choose the menu command Display gt Show gt Journal You may adjust the size of the Journal display with the mouse by dragging the boundary bar that separates the Journal from the Graph window www biopac com 28 JOURNAL TEX
56. ue Display allows incoming data values to be displayed numerically and or in bar chart format in a separate window during acquisition Checking this option enables you to open under the MP45 menu a Show Input Values window in which the values are displayed e Enable Value Display is useful for tracking slowly changing values such as heart rate respiration rate or the concentration of chemicals in a substance E Set up Channels x Channel Acquire Data Label Presets View lhange Plot on Screen Parameters Enable WYalue Display ANALOG INPUT CHANNELS CH e ECG 5 35 Hz cH2 Miei PPG 5 35 Hz CHS fw fv fe Stethoscope Heart Sounds IM Iv Iw at BSLSTM 0 100 vol Switch Temperature ideg C Temperature ideg F Temperature Change deg Temperature Change deg Torsiometer Pressure 2 5 co Accelerometer 5 g Accelerometer 50 g Airflow Adult Blood Pressure Cuff BNC f 10 to 10 Volts max BMC f 50 to 50 Volks max t Cardiac Output 2 Cardiac Output dZ Eco o gt Pressure 25 cm H20 Note the many presets available for data types you may wish to acquire Respiration PRO Tutorial 7 View Change Parameters W Set up Channels x Channel Acquire Data Label Presets View Change Parameters 15 E Click the View Change Parameters icon wrench for Analog Input Channel CH2 PPG 5 35 Hz Plot on Screen Enable Value Display
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