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Flame Miniature Spectrometer

1. i Voltage oe Pin Function Pavel Description 1 Ground N A Ground 2 Trigger 5or3 3V The TTL trigger signal 3 e Strobe 5V TTL output signal used to pulse a strobe that is divided down from the Master Clock signal 5V TTL output pulse used as a strobe signal which has a 4 Single Strobe programmable delay relative to the beginning of the spectrometer integration period 5V A TTL signal that is driven Active HIGH when the Lamp 5 Lamp Enable Enable command is sent to the Flame 6 GPIO 0 2 5 V General Purpose Software Programmable Digital Inputs Output 2 5 V General Purpose Software Programmable Digital 7 GPIO 1 Inputs Output 225 00000 000 11 201505b 49 Kocen 6 Technical Specifications UP Opi cs Pin Function MEETS Description Level 2 5V General Purpose Software Programmable Digital a GPIO 2 Inputs Output 2 5 V General Purpose Software Programmable Digital S GPIO 3 Inputs Output 2 5 V General Purpose Software Programmable Digital ja Ground Inputs Output 2 5 V General Purpose Software Programmable Digital n SES Inputs Output 2 5 V General Purpose Software Programmable Digital We GPIO 5 Inputs Output 2 5 V General Purpose Software Programmable Digital 13 GPIO 6 Inputs Output 2 5 V General Purpose Software Programmable Digital lu GREY Inputs Output 15 Ground N A Ground 16 SPI Master Clock 3 3 V Master clock See SPI below 3
2. Flame Miniature Spectrometer User Manual For Products FLAME S FLAME T Document 225 00000 000 11 201505b A HALMA COMPANY AMERICAS amp WORLD HEADQUARTERS Phone 1 727 733 2447 GE T Inc 733 ouglas Ave Fax 1 727 733 3962 Dunedin FL 34698 Sales info oceanoptics com USA Orders orders oceanoptics com Manufacturing amp Logistics Support techsupport oceanoptics com 4301 Metric Dr Winter Park FL 32792 USA EUROPE MIDDLE EAST amp AFRICA Phone 31 26 319 0500 Sales amp Support Fax 31 26 319 0505 Geograaf 24 Email info oceanoptics eu 6921 EW Duiven The Netherlands Germany 49 711 341696 0 i e UK y 44 1865 811118 Manufacturing amp Logistics Maybachstrasse 11 France 33 442 386 588 73760 Ostfildern Germany ASIA Phone 86 21 6295 6600 Ocean Optics Asia Fax 86 21 6295 6708 l 666 Gubei Road Email asiasales oceanoptics com Kirin Tower Suite 601B Changning District Japan amp Korea 82 10 8514 3797 Shanghai PRC 200336 www oceanoptics com Copyright 2015 Ocean Optics Inc All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted by any means electronic mechanical photocopying recording or otherwise without written permission from Ocean Optics Inc Trademarks All products and services herein are the trademarks service marks registered trademarks or registered service marks of their respective owners Lim
3. FLAME T Firmware Hardware Description The Flame utilizes a Cypress FX2 microcontroller which has a high speed 8051 combined with an USB ASIC Program code and data coefficients are stored in external E2PROM which are loaded at boot up via the 12C bus Spectral Memory Storage The Flame can store a single spectrum in the spectral data section While spectra are being accumulated it is being co added to the existing spectra in memory With this approach it is capable to accumulate any number of spectra previous limit was 4 USB Information Ocean Optics Vendor ID number is 0x2457 and the Product ID is 0x1022 Instruction Set Command Syntax The list of the commands is shown in the following table followed by a detailed description of each command The length of the data depends on the command All commands are sent to the Flame through End Point 1 Out EP1 All spectra data is acquired through End Point 2 and 6 In and all other queries are retrieved through End Point 1 In EP1 The endpoints enabled and their order is Hi Speed Size Full Speed Size Endpoint Pipe Description Type Bytes Bytes Address 0 End Point 1 Out Bulk 512 64 0x01 1 End Point 2 In Bulk 512 64 0x82 End Point 6 In Bulk 512 64 0x86 End Point 1 In Bulk 512 64 0x81 225 00000 000 11 201505b 93 8 Firmware and Advanced Communications USB Command Summary EP2 Command Byte Value 0x01 0x02 0x03 0x05 0x0
4. 250 mA at 5 VDC Supply voltage 4 75 5 25 V Power up time 2S Connectors Micro USB and JAE DD4 DD4RA40JA1 40 pin connector Micro USB Absolute Maximum Ratings Vcc 5 5 VDC DD4 Absolute Maximum Ratings Vcc Pin 40 5 5 VDC Voltage on any pin other than input power 4VDC Interface USB RS 232 USB 2 0 480 Mbps 2 wire RS 232 Mechanical Spectrometer Design Asymmetric crossed Czerny Turner Input Fiber Connector SMA 905 or FC Gratings 15 different gratings See Chapter 5 How the Flame Spectrometer Works for more information Entrance Slit 5 10 25 50 100 or 200 um slits Slits are optional In the absence of a slit the fiber acts as the entrance slit Physical Dimensions 88 9 mm x 63 5 mm x 31 9 mm Weight 265 g Environmental Temperature Storage 30 to 70 C Operation 0 to 50 C 46 225 00000 000 11 201505b Ocean Optics 6 Technical Specifications Specification FLAME S FLAME T Humidity 0 90 noncondensing Compliance Electrical CE FCC CISPR 11 2010 EMC 2004 108 EC and EN 61326 1 2013 Material RoHS Shock IEC 60068 2 64 Vibration IEC 60068 2 31 Manufacturing ISO 9001 1 Dynamic range for a single acquisition is a measure of the ratio of full signal to noise light level at the maximum integration time Dynamic range of the system is the range o
5. 72 225 00000 000 11 201505b Ocean Sem USB High Speed 480Mbps Packet Format 8 Firmware and Advanced Communications The data is read from EP2In The packet format is described below Packet End Point Bytes Pixels 0 EP2in 512 0 255 1 EP2ln 512 256 511 2 EP2ln 512 512 767 3 EP2In 512 768 1023 4 EP2In 512 1024 1279 5 EP2In 512 1280 1535 EP2in 512 7 EP2In 512 1792 2048 8 EP2In 1 Sync Packet The format for the first packet is as follows all other packets except the synch packet has a similar format except the pixel numbers are incremented by 256 pixels for each packet Packet 0 Byte 0 Byte 1 Byte 2 Byte 3 Pixel 0 LSB Pixel 0 MSB Pixel 1 LSB Pixel 1 MSB Byte 510 Byte 511 Pixel 255 LSB Pixel 255 MSB Packet 15 Synchronization Packet 1 byte Byte 0 0x69 225 00000 000 11 201505b 73 Ocean Sem 8 Firmware and Advanced Communications USB Full Speed 12Mbps Packet Format In this mode all data is read from EP2In The pixel and packet format is shown below Packet End Point Bytes Pixels 0 EP2In 64 0 31 1 EP2In 64 32 63 2 EP2In 64 64 95 EP2In 64 63 EP2in 64 2016 2047 64 EP2In 1 Sync Packet Packet 0 Byte 0 Byte 1 Byte 2 Byte 3 Pixel 0 LSB Pixel 0 MSB Pixel 1 LSB Pixel 2 MSB Byte 62 Byte 63 Pixel 31 LSB Pixel
6. 9us The user needs to account for this time delay when the pixels are optically inactive especially in the external triggering modes Timing Signals Strobe Signals Single Strobe The Single Strobe signal is a programmable TTL pulse that occurs at a user determined time during each integration period This pulse has a user defined High Transition Delay and Low Transition Delay The pulse width of the Single Strobe is the difference between these delays It is only active if the Lamp Enable command is active Synchronization of external devices to the spectrometer s integration period is accomplished with this pulse The Strobe Delay is specified by the Single Strobe High Transition Delay SSHTD and the Pulse Width is specified by the Single Strobe Low Transition Delay SSLTD minus the Single Strobe High Transition Delay PW SSLTD SSHTD Both values are programmable in 500ns increments for the range of 0 to 65 535 32 7675ms The timing of the Single Strobe is based on the Start of Integration SOI SOI occurs on the rising edge of 4ROG which is used to reset the Sony ILX511 detector In all trigger modes using an External Trigger there is a fixed relationship between the trigger and the SOI In the Normal mode and Software Trigger mode the SOI still marks the beginning of the Single Strobe but due to the nondeterministic timing of the software and computer operating system this timing will change over time and is not periodic
7. Byte 0 Ox6C Return Format EP1In Byte 0 Byte 1 Byte 2 Read Result ADC Value LSB ADC Value MSB If the operation was successful the Read Result byte value will be 0x08 All other values indicate the operation was unsuccessful Read Irradiance Factors Reads 60 bytes of data which is utilized for Irradiance Calibration information from the desired EEPROM memory address 225 00000 000 11 201505b 81 8 Firmware and Advanced Communications Byte Format Byte 0 Byte 1 Byte 2 Ox6D EEPROM Address LSB EEPROM Address MSB Return Byte Format Byte 0 Byte 1 Byte 59 Byte 0 Byte 1 Byte 59 Write Irradiance Factors Ocean Sem Write 60 bytes of data which is utilized for Irradiance Calibration information to the desired EEPROM memory address Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Byte 62 Ox6E EEPROM Address LSB EEPROM Address MSB Byte 0 Byte 59 Query Status Returns a packet of information containing the current operating information Packet structure is given below Byte Format Byte 0 OxFE Return Format The data is returned in Binary format and read in by the host through End Point 1 In The structure for the return information is as follows Byte Description Comments 0 1 Number of Pixels WORD LSB MSB order 2 5 Integration Time WORD Integr
8. C Since dark current is expressed as a rate the average baseline will go up as integration time is increased How rapidly the baseline increases with integration time is a function of temperature This graph provides an indication of what to expect Note that the y axis is a log plot 225 00000 000 11 201505b 53 Ocean Corr 6 Technical Specifications Spectral Resolution vs Wavelength 1 8 1 6 4 p N Spectral resolution nm Co Di 0 4 4 0 2 200 300 400 500 600 700 800 Wavelength nm Measured on a FLAME S UV VIS with a 25 um slit This is a plot of the spectral resolution of Flame UV VIS across the wavelength range of the device The spectral resolution is calculated by the FWHM of several peaks in an HG 1 spectrum mercury argon line source The resolution in nm is relatively flat across most of the spectral range CCD Overview CCD Detector The detector used for the Flame is a charge transfer device CCD that has a fixed well depth capacitor associated with each photodetector pixel Charge transfer reset and readout initiation begin with the integration time clock going HIGH At this point the remaining charge in the detector wells is transferred to a shift register for serial transfer This process is how the array is read The reset function recharges the photodetector welle to their full potential and allows for nearly continuous integration of the light energy during
9. External Triggering The Flame Spectrometer has several ways of acquiring data In the Normal Free Run mode the spectrometer is free running That is the spectrometer is continuously scanning acquiring and making data available to your computer according to parameters set in the software In this mode there is no way to synchronize the scanning acquisition and transfer of data with an external event However trigger pulses for synchronizing an external event with the spectrometer are available Each trigger mode involves connecting an external triggering device to the spectrometer and then applying an external trigger to the spectrometer before the software receives the data The length of the integration time and the source for the integration clock depend upon the mode chosen All other acquisition parameters are set in the software You can trigger the Flame using a variety of External Triggering options through the 40 pin Accessory Connector on the spectrometer See the External Triggering Options document located at_http oceanoptics com wp content uploads External Triggering Options Firmware3 Q0andAbove pdf The triggering document contains further instructions for configuring External Triggering options for the Flame Triggering Modes The Flame supports three triggering modes plus Normal mode which are set with the Trigger Mode command Detailed information of each triggering mode follows Also refer to the Externa
10. Read Register Information Read PCB Temperature Read Irradiance Calibration Factors Write Irradiance Calibration Factors Query Information USB Command Descriptions A detailed description of all Flame commands follows While all commands are sent to EP1 over the USB port the byte sequence is command dependent The general format is the first byte is the command value and the additional bytes are command specific values Byte 0 Byte 1 Byte 2 sta Byte n 1 Command Command Command D Command Byte Specific Specific Specific Initialize Flame Initializes certain parameters on the Flame and sets internal variables based on the USB communication speed the device is operating at This command should be called at the start of every session however if the user does not call it it will be executed on the first Request Scan command The default values are set as follows Parameter Default Value Trigger Mode O Normal Trigger Byte Format Byte 0 0x01 225 00000 000 11 201505b 69 Ocean Sm 8 Firmware and Advanced Communications Set Integration Time Sets the Flame integration time in microseconds The value is a 32 bit value whose acceptable range is 1 000us 65 535 000us If the value is outside this range the value is unchanged For integration times less than 655 000us the integration counter has a resolution of 10us For integration times greater than this t
11. See the Frequently Asked Questions in Chapter 4 Troubleshooting for more information on determining your computer version 10 225 00000 000 11 201505b Ocean ip Optics 2 Installation and Setup Software Windows Apple Linux S 2000 XP Vista 7 8 8 1 OceanView N N N N OSX Version 10 5 Any version released for or later on Intel an x86 or amd64 platform processor since 2010 SpectraSuite 4 N N N OS X Version 10 0 Red Hat 9 or later or later Fedora any version Debian 3 1 or later Sarge SUSE 9 0 or later Centos any version and Ubuntu About OceanView OceanView is the latest generation of operating software for all Ocean Optics spectrometers It is a Java based spectroscopy software platform that operates on Windows Macintosh and Linux operating systems The software can control any Ocean Optics USB spectrometer and device OceanView is a user customizable advanced acquisition and display program that provides a real time interface to a variety of signal processing functions With OceanView you have the ability to perform spectroscopic measurements such as absorbance reflectance and emission control all system parameters collect and display data in real time and perform reference monitoring and time acquisition experiments Consult the OceanView manual for hardware requirements when using OceanView see Product Related Documentation Hardware Setup
12. After the integration period the spectrum is retrieved and available to the user As in normal mode no further spectra are acquired until the original spectrum is read by the user External Hardware Level Trigger Mode In the External Hardware Level Trigger mode a rising edge detected by the spectrometer from the External Trigger input starts the integration period specified through the software interface After the integration period the spectrum is retrieved and is ready to be read by the user As long as the trigger level remains active in a logic one state back to back acquisitions can occur as in the Normal mode until the trigger transitions to an inactive level As in normal mode no further spectra are acquired until the original spectrum is read by the user External Hardware Edge Trigger Mode In the External Hardware Edge Trigger mode a rising edge detected by the spectrometer from the External Trigger input starts the integration period specified through the software interface After the integration period the spectrum is retrieved and is ready to be read by the user If another trigger is sent a new integration cycle will begin If a spectrum request is not received before the integration cycle has ended then that data will be deleted and a new trigger and spectrum request is required Only one acquisition will be performed for each External Trigger pulse no matter what the pulse s duration is No further spectra are acquired u
13. The Flame Spectrometer connects to a computer via the USB port or serial port When connected through a USB 2 0 or 1 1 port the spectrometer draws power from the host computer eliminating the need for an external power supply The Flame like all Ocean Optics USB devices can be controlled by our OceanView software see About OceanView 225 00000 000 11 201505b 11 2 Installation and Setup Ocean Sm DH 2000 BAL Computer Ocea W Flame Spectrometer QP450 0 25 XSR fiber QP450 0 25 XSR fiber Ocean Optics Flame Fiber Optic Spectrometer Typical Set up Follow the steps below to connect the Flame to a computer via the USB port 1 2 3 Install the spectrometer operating software on the destination computer Locate the USB cable CBL MICROTOA USB provided with the Flame Insert the micro connector end of the cable into the side of the Flame and the larger end of the cable into the USB port of the computer Connect any spectroscopy accessories To find operating instructions for Flame compatible products such as light sources sampling chambers and probes consult the Ocean Optics website at http oceanoptics com support technical documents Attach the fiber to the fiber optic connector on the spectrometer If you installed the spectrometer operating software prior to connecting the Flame the software automatically installs the Flame drivers If the drivers do not successfully install
14. e Choice of USB or RS 232 serial communications supported by drivers and software that make it easy to integrate into almost any system e 8 GPIO pins and the ability to provide power to external devices through the 40 pin connector Key Feature Example Applications User Users who wish to vary resolution and Life science and other labs using a wide Interchangeable throughput during measurements or variety of biological samples Slits switch from absorbance to fluorescence in minutes Indicator LEDs Convenient visual reference for Teaching and general lab use spectrometer operation and status Thermal Stability Applications that require repeatable LED binning and light metrology results in industrial and other process monitoring environments with varying temperatures and conditions Reduced Unit to OEM applications such as uncalibrated OEM manufacturers of analytical Unit Variation sensitivity and other measurement instrumentation needs where users benefit from low variance 2 225 00000 000 11 201505b Ocean Ear 1 Introduction Key Feature Example Applications Configurability Optimizing your spectrometer for Laser characterization low signal application specific requirements fluorescence and optimizing for specific adjusting range throughput and absorbance bands resolution and adding features such as filters as required Plug amp Play Users that want the convenience of Remote sensing measurements in the Operati
15. what should do Hardware device driver installation is usually seamless on Microsoft Windows operating systems and should happen in the background when you connect your spectrometer to a computer with the software installed However some Windows systems require a bit more care when connecting your spectrometer for the first time 225 00000 000 11 201505b 31 Ocean Serr If your spectrometer is not recognized by OceanView on your computer you need to manually install the spectrometer drivers See your OceanView manual for this procedure Also consult the Correcting Device Driver Issues document see_Product Related Documentation How do know my spectrometer has power The red LED on the spectrometer should be on steadily if the unit is receiving power 4 Troubleshooting How do know my spectrometer is transmitting data The green LED on the spectrometer flashes when transmitting data I m installing OceanView but need a product key where can find this The product key was sent to the contact e mail on the sales order when you purchased your OceanView license Contact info oceanoptics com for more information You ll need your sales order number quotation number the serial number of the spectrometer that was purchased with the software and if known the e mail address under which your product key was created to recover your key connected the Flame to the computer before installing my spectroscopy operating s
16. 1 Byte 2 ia Byte N 1 of Bytes N Read Byte 0 Read Byte 1 oF Read Byte N Write Register Information Most all of the controllable parameters for the Flame are accessible through this command e g GPIO strobe parameters etc A complete list of these parameters with the associate register information is shown in the table below Commands are written to End Point 1 Out typically with 4 bytes some commands may require more data bytes All data values are 16 bit values transferred in LSB MSB order This command requires 100us to complete the calling program needs to delay for this length of time before issuing another command In some instances other commands will also write to these registers i e integration time in these cases the user has the options of setting the parameters through 2 different methods Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Register Data Byte LSB Data Byte ES Value MSB 78 225 00000 000 11 201505b Ocean Optics 8 Firmware and Advanced Communications l Default Min Max Time Base Register Description Value Value Value Address 0x00 Master Clock Counter Divisor 24 1 OxFFFF 48MHz 0x04 FPGA Firmware Version Read Only Sen S i Continuous Strobe 0x08 GE trobe Timer Interval 48000 0 OxFEEF Base Clock see Register 0x0C 0x0C Continuous Strobe Base Clock Divisor 4800 0 OxFFFF 48MHz 0x0C Co
17. 2 order non linearity correction coefficient 9 3 order non linearity correction coefficient Valie 10 4 order non linearity correction coefficient 11 5 order non linearity correction coefficient 12 6 order non linearity correction coefficient 13 7 order non linearity correction coefficient 14 Polynomial order of non linearity calibration 15 Optical bench configuration gg fff sss gg Grating 4 fff filter wavelength sss slit size 16 Flame configuration AWL V A Array coating Mfg W Array wavelength VIS UV OFLV L L2 lens installed V CPLD Version 17 Reserved 18 Reserved 19 Reserved Response ACK or NAK Default value N A To query the constants use the x DATA WORD format to specify the desired constant Query Variable Returns the current value of the parameter specified The syntax of this command requires two ASCII characters The second ASCII character corresponds to the command character which 92 225 00000 000 11 201505b Ocean Soom sets the parameter of interest acceptable values are B A I K T J y A special case of this command is x lower case which requires an additional data word bee passed to indicate which calibration constant is to be queried 8 Firmware and Advanced Communications Command Syntax ASCII character Response ACK followed by DATA WORD Default value N A
18. 3 V The SPI Master Out Slave In MOSI signal for w PASE N communications to other SPI peripherals See SPI below 3 3 V TTL output signal used to pulse a strobe that is divided down CR SPI Master CS from the Master Clock signal See SPI below 3 3 V The SPI Master In Slave Out MISO signal for i SEET communications to other SPI peripherals See SP below 20 Ground N A Ground 21 CC Master Clock 3 3 V DC Master Clock See 12C below 22 CC Master Data 3 3V DC Master Data See 12C below 23 Ground N A Ground 24 RS232 TX 6 to 6 V RS232 Transmit signal for communication with PC connect 25 RS232 RX N A RS232 Receive signal for communication with PC connect 26 Reserved N A Reserved 50 225 00000 000 11 201505b Ocean Optics 6 Technical Specifications Pin Function ere Description 27 Reserved N A Reserved 28 Reserved N A Do not connect 29 Reserved N A Reserved 30 Reserved ES Reserved 31 Reserved Ke Reserved 32 Reserved NA Reserved 33 Reserved NA Reserved 34 Reserved WA Reserved 35 Reserved Ke Reserved 36 Reserved N A Reserved 37 Reserved NA Reserved 38 Reserved N A Do not connect 39 Ground N A Ground 5V Can be used either to draw power from output or supply power to input the Flame Internally the pin is connected to the output of a load switch driven by the 5V USB power supply Output The load switch soft starts the auxiliary power supply and pr
19. 31 MSB Packet 64 Synchronization Packet 1 byte Byte 0 0x69 Autonulling Slot 0x11 17 contains autonulling information that has a scaling term used to adjust the magnitude of the entire spectrum This can be read out by sending bytes 0x05 11 to the low speed out endpoint 0x01 and then reading out 17 bytes from the low speed in endpoint 0x81 The bytes of use are Byte offset 6 and 7 The 17 bytes will be formatted as follows 0x05 11 XX XX XX XX SS SS XX XX XX XX XX XX XX XX XX 74 225 00000 000 11 201505b Koco WP Optics 8 Firmware and Advanced Communications Where XX reserved bytes most are either unused or are only used internally to the device SS saturation level of the device as two bytes LSB followed by MSB These need to be assembled into a single 16 bit value Any time that a spectrum is read from the spectrometer each pixel s intensity value should be multiplied by 65535 0 saturation_level to set the scale appropriately The contents of slot 0x11 are set at the factory and should not be altered Set Trigger Mode Sets the Flame Trigger mode to one of four states If an unacceptable value is passed then the trigger state is unchanged refer to the External Triggering Options Instructions for a description of the trigger modes Data Value 0 3 Normal Free running Mode Data Value 1 gt External Hardware Level Trigger Mode Data Value 2 3 External Sy
20. 9 Ocean Sem 2 Installation and Setup Caution Be sure to install the software BEFORE connecting the spectrometer to your PC The software installs the drivers required for spectrometer installation If you do not install the software first the system will not properly recognize the spectrometer If you have already connected the Flame to a computer running on a Windows platform prior to installing the operating software consult Chapter 4 Troubleshooting for information on correcting a corrupt Flame installation gt Procedure Use the following procedure to get your system up and running quickly See Hardware Setup for more detailed information 1 Install your spectroscopy operating software by following the installation wizard prompts See Software Installation for more in depth information 2 Locate the micro USB cable provided with the spectrometer Connect the spectrometer to the USB port on your computer using this cable USB Port 3 Take measurements Refer to your spectrometer operating software manual for information on the software user interface Software Installation Use OceanView version 1 5 and above for Flame Flame can be used with SpectraSuite but will appear as a USB2000 Spectrometer in the software You can use OceanView or SpectraSuite on the following operating systems Caution Be sure that you download the correct software package for your computer version 32 or 64 bit
21. DATA WORD 2 Data Word 1 FPGA Register address Value Data Word 2 FPGA Register Value Response ACK or NAK Default value N A ASCII Data Mode Sets the mode in which data values are interpreted to be ASCII Only unsigned integer values 0 65535 are allowed in this mode and the data values are terminated with a carriage return ASCII 13 or linefeed ASCII 10 In this mode the Flame echoes the command and data values back out the RS 232 port 90 225 00000 000 11 201505b Ocean port ICS 8 Firmware and Advanced Communications Command Syntax aA Response ACK or NAK Default value N A The command requires that the string aA be sent without any CR or LF This is an N attempt to insure that this mode is not entered inadvertently A legible response to the Version number query v command indicates the Flame is in the ASCII data mode Binary Data Mode Sets the mode in which data values are interpreted to be binary Only 16 bit unsigned integer values 0 65535 are allowed in this mode with the MSB followed by the LSB Command Syntax bB Response ACK or NAK Default value Default at power up not changed by Q command The command requires that the string bB be sent without any CR or LF This is an attempt to insure that this mode is not entered inadvertently Checksum Mode Specifies whether the Flame will g
22. Setup exe file to install the software The Welcome screen appears Click the Next button The Destination Location screen appears Accept the default installation location or click the Browse button to specify a directory Then click the Next button The Program Manager Group screen appears Click the Next button The Start Installation screen appears 8 Click the Next button to begin the installation Once the installation finishes the 10 11 12 13 Installation Complete screen appears Click the Finish button and reboot the computer when prompted Navigate to the USB EEPROM Programmer from the Start menu and run the software Click on the desired Flame device displayed in the left pane of the USB Programmer screen Double click on each of the calibration coefficients displayed in the right pane of the USB Programmer screen and enter the new values acquired in Steps 5 and 6 of the Calibrating the Wavelength of the Spectrometer section in this appendix Repeat Step 12 for all of the new values 225 00000 000 11 201505b Ocean VW beier Sex 7 Calibration 14 Click on the Save All Values button to save the information and then Exit the USB Programmer software The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the Flame lrradiance Calibrations Irradiance calibrations and relative irradiance calibrations are about quantifying the spectra by translating the signal in
23. Spectrometer Works Grating Intended Use Groove Density Spectral Range Blaze Wavelength Best Efficiency Number gt 30 aa UV VIS 2400 s0 1200m 120 nm Holographic VIS Holographic Vis 260 780nm 260780nm nm Grating efficiency curves are available to view by using our online range and resolution calculator at www oceanoptics com product category modular spectrometers Gratings Showing Light Diffracted into its Constituent Wavelengths 6 Focusing Mirror specify standard or SAG This mirror focuses first order spectra on the detector plane Both the collimating and focusing mirrors are made in house to guarantee the highest reflectance and the lowest stray light possible You can opt to install a standard or SAG mirror As with the collimating mirror the mirror type needs to be specified when ordering 7 Detector Collection Lens optional This cylindrical lens is fixed to the detector to focus the light from the tall slit onto the shorter detector elements It increases light collection efficiency and reduces stray light It also is useful in a configuration with a large diameter fiber and slit for low light level applications such as fluorescence Preconfigured Flame spectrometers with a collector lens are available look for ES at the end of the name 8 Detector There are two choices of detector available for the flame We offer a 2048 element FLAME S Sony ILX511B or a 3648 element FLAME
24. Wor ics Byte Format Byte 0 Byte 1 0x05 Configuration Index Return Format EP1 The data is returned in ASCII format and read in by the host through End Point 1 Byte 0 Byte 1 Byte 2 Byte 3 Byte 16 0x05 Configuration Index ASCllbyte0 ASCII byte1 ASCII byte 14 Write Information Writes any of the 19 stored spectrometer configuration variables to EEPROM The 19 configuration variables are indexed as described in the Query Information The information to be written is transferred as ASCII information Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Byte 16 0x06 Configuration Index ASCII byte 0 ASCII byte 1 ASCII byte 14 Request Spectra Initiates spectra acquisition The Flame will acquire a complete spectrum 2048 pixel values The data is returned in bulk transfer mode through EP2 The table below provides the pixel order overview for the 2 different speeds The pixel values are decoded as described below Byte Format Byte 0 0x09 Return Format The format for the returned spectral data is dependent upon the USB communication The format for both High Speed 480 Mbps and Full Speed 12Mbps is shown below All pixel values are 16 bit values which are organized in LSB MSB order There is an additional packet containing one value that is used as a flag to insure proper synchronization between the PC and Flame
25. block second and third order light from reaching specific detector elements Light reflected off the grating can propagate 2 and 3 order effects at whole multiples of the incident light While these signals are weak they may cause stray light that reduces the accuracy of the spectrometer response Order sorting filters reject this stray light only allowing the desired wavelength through to the detector Order sorting filters are combined with detector window upgrades The full range available is listed below These must be specified at the time of ordering Detector Window Upgrades optional The standard BK7 glass window on the detector begins to absorb light around 340nm For applications in the UV below 360nm we recommend the detector window upgrade This replaces the BK 7 glass with Quartz Typically these are used in conjunction with an order sorting filter to block the impact of 2 4 and 3 order effects at higher wavelengths 225 00000 000 11 201505b Ocean Sem 5 How the Flame Spectrometer Works Available Order Sorting and Detector Window Options Detector Description Spectrometer DET2B 200 535 Sony ILX511B detector installed with Custom OFLV Coated FLAME S Window Assembly for Grating 5 and Grating 5U S bench DET2B 200 850 Sony ILX511 detector installed with 200 850 nm variable FLAME S longpass filter and UV2 quartz window best for UV VIS systems configured with G
26. further understand your state of the art products and how they function located at http oceanoptics com glossary Document Version Document Number Version 225 00000 000 11 201503 First release 225 00000 000 11 201503b Updates technical specifications troubleshooting instructions and other minor issues 225 00000 000 11 201504 Updates the Electrical pinout information 225 00000 000 11 201505 Adds information for the Performance Charts 225 00000 000 11 201505b Updates information for changing the slit DD4 accessory connector pinouts and firmware commands Patent Pending Notice The Flame spectrometer is covered by Patents Pending Any violation of Ocean Optics intellectual property will be prosecuted Warranty Our 3 Year Warranty covers Ocean Optics miniature fiber optic spectrometers light sources and sampling accessories regardless of the application from manufacturing defects It also covers fibers and probes for a full 12 months http oceanoptics com services exclusive 3 year warranty vi 225 00000 000 11 201505b Ocean Ear This comprehensive warranty ensures you of the highest level of craftsmanship and reliability for years to come No other manufacturer offers such a solid guarantee of quality and reliability The Ocean Optics 3 Year Warranty applies to Ocean Optics equipment excluding OEM configurations purchased on or after July 1 2010 The warranty cover
27. in the wavelength region of your spectrometer e A Flame spectrometer e An optical fiber for spectrometers without a built in slit a 50 um fiber works best e A spreadsheet program Excel or Quattro Pro for example or a calculator that performs third order linear regressions Note If you are using Microsoft Excel choose Tools Add Ins and check AnalysisToolPak and AnalysisTookPak VBA Calibrating the Wavelength of the Spectrometer gt Procedure Perform the steps below to calibrate the wavelength of the spectrometer 1 Place the spectrometer operating software into Quick View Scope mode and take a spectrum of your light source Adjust the integration time or the A D conversion frequency until there are several peaks on the screen that are not off scale 2 Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum intensity 3 Record the pixel number that is displayed in the status bar or legend located beneath the graph Repeat this step for all of the peaks in your spectrum 4 Use the spreadsheet program or calculator to create a table like the one shown in the following figure In the first column place the exact or true wavelength of the spectral lines that you used 62 225 00000 000 11 201505b Ocean VW optics 7 Calibration In the second column of this worksheet place the observed pixel number In the third column calculate the pixel number squared and i
28. is transmitted immediately Command Syntax S Response If successful STX followed by data If unsuccessful ETX 225 00000 000 11 201505b 89 Ocean Sem The format of returned spectra includes a header to indicate scan number channel number pixel mode etc The format is as follows WORD OxFFFF start of spectrum WORD Data size flag 0 gt Data is WORD s 1 gt Data is DWORD s WORD Number of Scans Accumulated WORD Integration time in milliseconds WORD FPGA Established Baseline value MSW WORD FPGA Established Baseline value LSW WORD pixel mode WORDs if pixel mode not 0 indicates parameters passed to the Pixel Mode command P D WORDs spectral data depending on Data size flag WORD OxFFFD end of spectrum 8 Firmware and Advanced Communications Trigger Mode Sets the Flame s external trigger mode to the value specified Command Syntax T DATA WORD O Normal Free running Mode 1 Software Trigger Mode Value 2 External Hardware Level Trigger Mode 3 External Synchronization Trigger Mode 4 External Hardware Edge Trigger Mode Response ACK or NAK Default value 0 Set FPGA Register Value Sets the appropriate register within the FPGA The list of register setting is in the USB command set information This command requires two data values one to specify the register and the next to specify the value Command Syntax W DATA WORD 1
29. it is determined that the product must be returned the representative will issue an RMA number 2 Package your product ideally in the original packaging and return it to Ocean Optics along with the RMA number that you received Note For RMA returns under warranty we will organize and pay for shipping both ways For accidental damage you only pay to have the product delivered to your closest Ocean Optics or OOI Distributor Office 34 225 00000 000 11 201505b Ocean kaf Ree ooo Upon careful examination well advise you with an estimate When your product is ready it will be returned to you Servicing To keep your instrument in tip top shape we recommend yearly wavelength recalibration You can do this yourself if you have appropriate tools or we can do this for you Contact your local representative to find out more about service availability and cost We offer the following services e Wavelength Calibration e Absolute Irradiance Calibrations 225 00000 000 11 201505b 35 2Ocean 4 Troubleshooting e AE EICH 36 225 00000 000 11 201505b Chapter 5 How the Flame Spectrometer Works Overview This section provides an overview of the Flame spectrometer and how it works from light entering the slit through to the transmission of the spectrum over USB It also provides an overview of all the different possible configurations that are possible designed to help you optimize your spectrometer for specific applica
30. memory address Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Byte 62 EEPROM EEPROM Byte 0 Byte 59 Ox6E Address LSB Address MSB Query Status Returns a packet of information containing the current operating information The structure of the status packet is given below Byte Format Byte 0 OxFE Return Format The data is returned in Binary format and read in by the host through End Point 1 In The structure for the return information is as follows Byte Description Comments 0 1 Number of Pixels WORD LSB MSB order 2 5 Integration Time WORD Integration time in us LSW MSW Within each word order is LSB MSB 106 225 00000 000 11 201505b Ocean port ICs 8 Firmware and Advanced Communications Byte Description Comments 6 Lamp Enable 0 Signal LOW 1 Signal HIGH 7 Trigger Mode Value 8 Spectral Acquisition Status 9 Packets In Spectra Returns the number of Packets in a Request Spectra Command 10 Power Down Flag 0 Circuit is powered down 1 Circuit is powered up 14 Packet Count Number of packeis that have been loaded into End Point Memory 12 Reserved 13 Reserved 14 USB Communications Speed 0 Full Speed 12Mbs 0x80 High Speed 480 Mbps 15 Reserved FLAME T Serial Port Interface Communications and Control Information The Flame is a microcontroller based Miniatu
31. schematic view They are all various functions that take data in and provide an output To make a node right click on the schematic background Each node can be configured by double clicking on the node To join nodes press ctrl click and drag windows 5 Views Are a type of window that displays data To generate a new view right click on the schematic background More information about schematic view including detailed descriptions of the available nodes can be found in the Ocean View Installation and Operation Manual see Product Related Documentation and in the help section of the OceanView software 30 225 00000 000 11 201505b Chapter 4 Troubleshooting Overview Sometimes things dont quite go to plan hopefully you ll find some answers below If not dont hesitate to contact us and our Tech Support team will leap into action Some typical questions are answered here For more information consult the FAQs on the Ocean Optics website at http oceanoptics com faq connected the USB cable and started OceanView but don t see my spectrometer attached Use the Rescan button in the Device Manager to rescan for attached devices Device Manager x EARCH Use tection 0lFlame 5 RH use 0 Device Actions Disconnect Add Device Rescan IV Simulate device if none found JW Automatically connect to devices Rescan Button am having trouble installing the drivers
32. utilizes a Cypress CY7C68013 microcontroller that has a high speed 8051 combined with an USB2 0 ASIC Program code and data coefficients are stored in external EPROM that are loaded at boot up via the DC bus The microcontroller has 16K of internal SRAM and 64K of external SRAM Maximum throughput for spectral data is achieved when data flows directly from the external FIFO s directly across the USB bus In this mode the 8051 does not have access to the data and thus no manipulation of the data is possible USB Information Ocean Optics Vendor ID number is 0x2457 and the Product ID is 0x101E Instruction Set Command Syntax The list of the commands is shown in the following table followed by a detailed description of each command The length of the data depends on the command All commands are sent to the Flame through End Point 1 Out EP1 All spectra data is acquired through End Point 2 In and all other queries are retrieved through End Point 1 In EP1 The endpoints enabled and their order is 225 00000 000 11 201505b 67 8 Firmware and Advanced Communications 68 EP1 Command Byte Value Flame S Endpoints SS Detect Plug ins LED Status General UC Read Ocean C Endpoint Address 225 00000 000 11 201505b Kocen WP Opti ICS A ee 8 Firmware and Advanced Communications Communications EP1 Command Description Byte Value oe oi General C Write 0x62 General SPI I O Write Register Information
33. 01505b 101 Ocean Sem 8 Firmware and Advanced Communications Return Byte Format Byte 0 C Results General SPI Input Output Performs a general purpose write and read on the SPI bus for interfacing to attached peripherals The time to complete the command is determined by the amount of data transferred and the response time of the peripheral The SPI bus runs at 25KHz Clock The maximum number of bytes that can be written or read is 61 During this transfer the SPI Chip Select signal is driven to an active LOW TTL level Data is transmitted out the MOSI Master Out Slave In line on the rising edge of the clock signal Data is also latched in the from the MISO line on the falling edge of the clock signal Command Byte Format Byte 0 Byte 1 Byte 2 Byte 3 az Byte N 2 0x62 of Bytes N Write ByteO Write Byte 1 ba Write Byte N Return Byte Format Byte 0 Byte 1 Byte 2 Byte 3 es Byte N 1 of Bytes N Read Byte 0 Read Byte 1 Read Byte2 Read Byte N Write Register Information Most all of the controllable parameters for the Flame are accessible through this command e g GPIO strobe parameters etc A complete list of these parameters with the associate register information is shown in the table below Commands are written to End Point 1 Out typically with 4 bytes some commands may require more data bytes All data values are 16 bit value
34. 08 Command WR let le EE 109 Examples cca acea a tt a a i a a a a a a tt a at at anette cae 117 MAO APR ION PRE NOR NR SRR RR IRI PN A II RN NP RR 119 iv 225 00000 000 11 201505b About This Manual Document Purpose and Intended Audience Thank you for choosing Ocean Optics We hope that you ll be delighted with your decision This document provides the users of Flame Spectrometers with instructions for setting up calibrating and performing experiments with their spectrometer It also contains detailed technical specifications and information about firmware and hardware integration If you cant find what you re looking for in this document please do not hesitate to contact us at techsupport oceanoptics com or via www oceanoptics com Document Summary Chapter Description Chapter 1 Introduction Introduces the product features Contains descriptive information about the Flame Spectrometer It also provides a list of system requirements typical applications and product versions Chapter 2 Installation and Setup Provides installation instructions including how to set up the Flame with OceanView Also includes package contents and typical set ups for different measurement techniques Chapter 3 Flame Operation with Describes how to use the Flame with OceanView software OceanView including how to connect acquire save and other basic features Chapter 4 Troubleshooting Contains recommended steps to is
35. 4 Troubleshooting Sem How do determine whether my Windows computer is 32 bit or 64 bit Errors can occur if you download the wrong version of software for 32 bit or 64 bit computers To verify your computer version for most Windows computers go to the Properties window under Computer or My Computer If no version is listed then your system is a 32 bit For more information see the Microsoft Frequently Asked Questions at http windows microsoft com en us windows 32 bit and 64 bit windows 1TC windows 7 How do check the configuration of my spectrometer Check the label on the bottom of your spectrometer You can also check your configuration using your spectrometer operating software For OceanView Open the Schematic window and double click the spectrometer icon For SpectraSuite Click the plus icon next to the spectrometer to open properties and configuration Product Upgrades Repairs and Servicing Occasionally you may find that you need Ocean Optics to make a change or an upgrade to your system To facilitate these changes you must first contact Customer Support and obtain a Return Merchandise Authorization RMA number Please contact Ocean Optics for specific instructions when returning a product Repairs Sometimes accidents happen If you need to return your Ocean Optics Product for repair here is what to do gt Procedure 1 Contact us to speak to an Ocean Optics representative about the problem If
36. 6 NI SS NN SSES USB Command Descriptions Ocean CES ICS A detailed description of all Flame commands follows While all commands are sent to EP1 over the USB port the byte sequence is command dependent The general format is the first byte is the command value and the additional bytes are command specific values 94 225 00000 000 11 201505b Kocen eo Opi ics 8 Firmware and Advanced Communications Byte 0 Byte 1 Byte 2 Byte n 1 Command Command Command Command Byte Specific Specific Specific Initialize Flame Initializes certain parameters on the Flame and sets internal variables based on the USB communication speed the device is operating at This command should be called at the start of every session however if the user does not call it it will be executed on the first Request Scan command The default vales are set as follows Parameter Default Value Trigger Mode 0 Normal Trigger Byte Format Byte 0 0x01 Set Integration Time Sets the Flame integration time in microseconds The value is a 32 bit value whose acceptable range is 10 65 535 000us If the value is outside this range the value is unchanged For integration times less than 655 000us the integration counter has a resolution of 10us For integration times greater than this the integration counter has a resolution of 1ms Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Byte 4 0x02 LSW L
37. 7 16 298 b 2015 15 27 16 308 ali Li W USB4C022371_15 40 05 719 b 2015 15 40 10 813 am rng ia USB4CO22371_15 32 24 457 04 pag Feb 2015 15 32 24 467 WS ISubt2_15 21 33 515 bt 04 Feb 2015 15 21 33 536 5 bad Ng Subt2_15 21 55 483 b X Ni ES kW geg e E 5 e E E S Wavelength nm Saved Data Panel 225 00000 000 11 201505b 27 Ocean 3 Operation WS Optics 1 Saved Files List of saved files currently in the saved directory Arrange by name or date 2 Preview Shows a preview of the saved spectra time series or appended series saved data that can be stepped through acquisition by acquisition using the controls above the saved files list 3 File Path Set the file directory 4 Overlay Set the previewed spectra as an overlay on the active view 5 Notes Enter notes about the saved spectra Notes are saved with the same file name as a separate tsv file These can be viewed or edited with any text viewer such as Notepad Projects and Methods OceanView makes it easy to save and load projects and methods We define a project as a measurement set up made with a particular spectrometer If the software cannot find the spectrometer it will load this as a method and prompt the user to select a substitute spectrometer from those selected Click to save a project Alternatively select File Save Project from the menu Saves all view and schematic parameters to a single ASC
38. 9 3 order non linearity correction coefficient 10 4 order non linearity correction coefficient 11 5 order non linearity correction coefficient 12 6 order non linearity correction coefficient 13 7 order non linearity correction coefficient 14 Polynomial order of non linearity calibration 15 Optical bench configuration gg fff sss gg Grating fff filter wavelength sss slit size 16 Flame configuration AWL V A Array coating Mfg W Array wavelength VIS UV OFLV L L2 lens installed V CPLD Version 17 Autonulling information 18 Power up baud rate value 19 30 User configured Byte Format Byte 0 Byte 1 0x05 Data byte 96 225 00000 000 11 201505b Ocean Sem Return Format EP1 8 Firmware and Advanced Communications The data is returned in ASCII format and read in by the host through End Point 1 Byte 0 Byte 1 Byte 2 Byte 3 0x05 Configuration Index ASCII byte 0 ASCII byte 1 Write Information Writes any of the 31 stored spectrometer configuration variables to EEPROM The 31 configuration variables are indexed as described in the Query Information The information to be written is transferred as ASCII information Byte Format Byte 0 Byte1 Byte 2 Byte 3 Byte 17 0x06 Configuration Index ASCII byte 0 ASCII byte 1 ASCII byte 15 Request Spectra Initi
39. Ds Off Data Byte 1 gt LEDs On Byte Format Byte 0 Byte 1 0x12 Data byte General I2C Read Performs a general purpose read on the DC pins for interfacing to attached peripherals The time to complete the command is determined by the amount of data transferred and the response time of the peripheral The DC bus runs at 400KHz The maximum number of bytes that can be read is 61 Command Byte Format Byte0 Byte 1 Byte 2 0x60 IC Address Bytes to Read 76 225 00000 000 11 201505b Ocean port ICS 8 Firmware and Advanced Communications Return Byte Format Byte 0 Byte 1 Byte 2 Byte 3 T Byte N 3 CC Results IC Address Bytes to Read Data Byte0 Data byte N 2C Result Value Description 0 DC bus Idle 1 IC bus Sending Data 2 CC bus Receiving Data 3 CC bus Receiving first byte of string 5 CC bus in waiting for STOP condition 6 DC experienced Bit Error 7 CC experience a Not Acknowledge NAK Condition 8 C experienced successful transfer 9 CC bus timed out General 12C Write Performs a general purpose write on the DC pins for interfacing to attached peripherals The time to complete the command is determined by the amount of data transferred and the response time of the peripheral In all I2C communications the first byte of the transaction consists of a 7 bit address and a read write bit The addr
40. Flame should be compressed to speed data transfer rates Command Syntax G DATA WORD Response ACK or NAK 0 Compression off Range 10 Compression on Default value 0 Integration Time 16 bit Sets the Flame s integration time in milliseconds to the value specified Command Syntax I 16 bit DATA WORD Response ACK or NAK Range 1 65000 Default value 10 86 225 00000 000 11 201505b Ocean Som 8 Firmware and Advanced Communications Integration Time 32 bit Sets the Flame s integration time in microseconds to the value specified Command Syntax i 32 bit DATA WORD Response ACK or NAK Range 1000 65 000 000 Default value 10 000 Lamp Enable Sets the Flame s Lamp Enable line to the value specified Command Syntax J DATA WORD O Light source strobe ot Lamp Enable low Value 1 Light source strobe on Lamp Enable high Response ACK or NAK Default value 0 Baud Rate Sets the Flame s baud rate Command Syntax K DATA WORD 0 2400 1 4800 2 9600 Value 3 19200 4 38400 5 Not Supported 6 115 200 Response See below Default value 2 When changing baud rates the following sequence must be followed 1 Controlling program sends K with desired baud rate communicating at the old baud rate 2 The Flame responds with AC
41. I cean Som TI II ERE III DR CE 0 DI EEE e Oxygen USB LS 450 related commands CR Jesse SE Command Descriptions A detailed description of all Flame commands follows The indicates a data value which is interpreted as either ASCII or binary default The default value indicates the value of the parameter upon power up Add Scans 8 Firmware and Advanced Communications Sets the number of discrete spectra to be summed together Since the Flame has the ability to return 32 bit values overflow of the raw 16 bit ADC value is not a concern Command Syntax A DATA WORD Response ACK or NAK Range 1 5000 Default value 1 225 00000 000 11 201505b 85 8 Firmware and Advanced Communications Pixel Boxcar Width Ocean Sm Sets the number of pixels to be averaged together A value of n specifies the averaging of n pixels to the right and n pixels to the left This routine uses 32 bit integers so that intermediate overflow will not occur however the result is truncated to a 16 bit integer prior to transmission of the data This math is performed just prior to each pixel value being transmitted out Values greater than 3 will exceed the idle time between values and slow down the overall transfer process Command Syntax B DATA WORD Response ACK or NAK Range 0 15 Default value 0 Set Data Compression Specifies whether the data transmitted from the
42. II file Wi Load a project or method Spectroscopy Application Wizards OceanView provides you with a wealth of functionality to capture store and process your data This chapter contains descriptions of wizards and other functionality See your OceanView manual for more information on wizards A Click this button to set up a measurement using simple step by step wizards A large range of applications is available Application Wizard Window 28 AOceanView Set up a Spectroscopy Application with our Spectroscopy Wizards SR revision a anal in 225 00000 000 11 201505b Ocean QOR 3 Operation Dark and Reference Measurements Dark and reference measurements are commonly used in spectroscopy e Dark Measurements Subtract a background signal from the spectrum This can be considered the removal of a constant error Typically this is done when the light source is off to remove any background from the ambient environment hence the name dark e Reference Measurements Make the signal relative to the reference Consider this a normalization of the signal against a reference Typically this is taken with a reference sample and the light source turned on This lets you look at the relative spectral change when a sample interacts with the illumination source Most often you will set up your measurement with the reference and dark through the application wizards The wizards will pro
43. K at old baud rate otherwise it responds with NAK and the process is aborted 3 Controlling program waits longer than 50 milliseconds 4 Controlling program sends K followed by the desired baud rate For example to change the baud rate from 9600 to 115 200 in ASCII mode e At 9600 baud send K6 lt enter gt e You will receive an ACK at 9600 baud 225 00000 000 11 201505b 87 Kocen 8 Firmware and Advanced Communications for ics e Send K6 lt enter gt at 115200 Note that the K is not echoed back but the 6 is e You will receive two ACK characters and the prompt at 115200 baud 4 The Flame responds with ACK at new baud rate otherwise it responds with NAK and old baud rate is used If a deviation occurs at any step the previous baud rate is utilized a Clear Memory Clears the spectral data memory based on the valued specified Clearing memory is immediate since only pointer values are reinitialized Caution All stored spectra are lost when the Clear memory command is executed Command Syntax L DATA WORD 0 Clear spectral memory Value 1 Clear spectral memory Response ACK or NAK Default value N A Data Storage Mode Sets the data storage mode for future spectral acquisitions Command Syntax M DATA WORD 0 Scans transmitted through the serial port Value 1 Scans stored in spectral memory and not transmitted Response ACK or NAK Default
44. Ocean Optics also offers a range of FC connector slits in the same wavelengths with the product code INTFC XXX An INTFC KIT is also available Note that these items are made to order and have a longer lead time Contact an Ocean Optics Application Sales Engineer for more details gt Procedure To calculate the optical resolution for your spectrometer 1 Find the number of pixels for your detector 2 Divide the range of the spectrometer by the number of pixels 3 Multiply this number by the pixel resolution from the table above For example Resolution of the Flame S with a 50 um slit and 650 nm range 650 2048 x 6 5 2 1 nm 225 00000 000 11 201505b Ocean QORN 3 LongPass Absorbing Filter optional If selected an absorbing filter is installed between the slit and the aperture in the SMA 905 bulkhead The filter is used to limit bandwidth of light entering spectrometer or to balance color Filters are installed permanently A filter is for a specific slit If you anticipate needing the filter with multiple slit sizes then you must specify this at the time you order You will know which filter is installed in each slit because of the color coded dots on the outside as shown in the figure and described in the table below 5 How the Flame Spectrometer Works 10 Sa ltem Code Description Dot 1 Dot 2 OF1 BG28 Bandpass filter transmits gt 325 and lt 500 n
45. RS 232 port Command Syntax aA Response ACK or NAK Default value N A Note This command requires that the string aA be sent without any CR or LF This is an attempt to ensure that this mode is not entered inadvertently A legible response to the version number query v command indicates the Flame is in the ASCII data mode Binary Data Mode Sets the mode in which data values are interpreted to be binary Only 16 bit unsigned integer values 0 65535 are allowed in this mode with the MSB followed by the LSB Command Syntax bB Response ACK or NAK Default value Default at power up not changed by Q command Note The command requires that the string bB be sent without any CR or LF This is an attempt to insure that this mode is not entered inadvertently Checksum Mode Specifies whether the Flame will generate and transmit a 16 bit checksum of the spectral data This checksum can be used to test the validity of the spectral data and its use is recommended when reliable data scans are required See Technical Note 2 for more information on checksum calculation Command Syntax k DATA WORD 0 Do not transmit checksum value Value i 10 transmit checksum value at end of scan Response ACK or NAK Default value 0 225 00000 000 11 201505b 115 8 Firmware and Advanced Communications Version Number Query Returns
46. SB LSW MSB MSW LSB MSW LSB MSW amp LSW Most Least Significant Word MSB amp LSB Most Least Significant Byte Set Strobe Enable Status Sets the Flame Lamp Enable line J2 pin 4 as follows The Single Strobe and Continuous Strobe signals are enabled disabled by this Lamp Enable Signal Data Byte 0 gt Lamp Enable Low Off Data Byte 1 gt Lamp Enable HIGH On 225 00000 000 11 201505b 95 Koco 8 Firmware and Advanced Communications Tee ics Byte Format Byte 0 Byte 1 Byte 2 0x03 Data byte LSB Data Byte MSB Query Information Queries any of the 31 stored spectrometer configuration variables The Query command is sent to End Point 1 Out and the data is retrieved through End Point 1 In When using Query Information to read EEPROM slots data is returned as ASCII text However everything after the first byte that is equal to numerical zero will be returned as garbage and should be ignored The 31 configuration variables are indexed as follows Data Byte Description 0 Serial Number 1 0 order Wavelength Calibration Coefficient 2 1 order Wavelength Calibration Coefficient 3 2 order Wavelength Calibration Coefficient 4 3 order Wavelength Calibration Coefficient 5 Stray light constant 6 0 order non linearity correction coefficient 7 1 order non linearity correction coefficient 8 2 order non linearity correction coefficient
47. T Toshiba TCD1304AP linear CCD array These both have an effective range of 190 1100 nm The optics split the light into its component wavelengths which fall across the different 225 00000 000 11 201505b 41 5 How the Flame Spectrometer Works 42 Ocean Som pixels Each pixel responds to the wavelength of light that strikes it The detector outputs an analog signal from each pixel that is converted via the ADC into a digital signal The driver electronics process this signal and send the spectrum via the USB connection to the software The best choice of detector will depend on the application Detector Specifications Specification S Type FLAME S T Type FLAME T Detector Sony ILX511B linear silicon CCD Toshiba TCD1304AP linear silicon array CCD array Strengths e Strong response lt 350nm e Slightly higher SNR due to well good for UV measurements depth e Fast data output rate e Larger number of pixels can L ixel size i offer better resolution with e arger pixel size improves small slits sensitivity e Electronic shutter Watch for N A Offers strong all around e Signal lag at low integration performance times e Signal may bleed to neighboring pixels at high intensities blooming e Higher minimum integration time See Chapter 6 Technical Specifications for more detailed detector specifications OFLV Variable Longpass Order sorting Filter optional Our proprietary filters precisely
48. Tele e TEEN 14 DB15 Connector Cable FLAME CBL DD4P DB15P nenea nenea aaa ana na 15 PAK50 Connector Cable FLAME CBL DD4P PAK50P nene nenea nea ana na 16 Breakout Box HR4 BREAKOUT men nn nea aaa amana aaa ana ana 16 Interchangeable SIits sariini e Va aaa pa o dl 16 Light Sources Cuvette Holders and Other Accessories mmm eee nne eee nenene 17 Measurement Techniques Typical Set ups ENNEN 17 Vaeste dors Lu 17 Common UV Vis Applications nenea nana aa ana ana aaa ana aaa 18 225 00000 000 11 201505b i Ocean Sem Table of Contents Reflectance and Transmission mean eee emana amana enma ee ana 18 Common UV Vis Reflectance Applications men nenea aaa ana nana 18 Common UV Vis Transmission Applications ce nene nenea aaa ana nana 18 FIUOrESCENGE scurs iai ti ta atacat lac aa Dat tal ti aia ata a et z 19 Common Fluorescence Applications nenea ana nn eaanaae ana a n aan 19 lte ne 20 Common Irradiance Applications cena nenea nn ame aanaa anaae nnen nenn 21 Chapter 3 Flame Operation with OceanView ssceceeeeeeeeeeees 23 OVETVI EW eege a n a n rad 23 ERT 23 OceanView Main Serena cine a e ic e i meenet 24 Connect the Flame in OceanView nene nene manea ana nn aaa ana aaa 25 Set Acquisition Parameters c men nea nenea nana ana manea anna nana aaa 25 Quick View and Device Response nenea na ana ana aa
49. That is at a constant integration time the Single Strobe will not be periodic but it will indicate the start of the integration The timing diagram for the Single Strobe in External Hardware Trigger mode is shown below The Trigger Delay TD is another user programmable delay which specifies the time in 500ns increments that the SOI will be delayed beyond the normal Start of Integration Delay SOID An example calculation of the Single Strobe timing follows If the TD ims SSHTD 50ms and SSLTD 70ms then the rising edge of the Single Strobe will occur approximately 51 82ms 1ms 50ms 8 2us after the External Trigger Input goes high and the Pulse Width will be 20ms 70ms 50ms Continuous Strobe The Continuous Strobe signal is a programmable frequency pulse train with a 50 duty cycle It is programmed by specifying the desired period whose range is 2us to 60s This signal is continuous once enabled but is not synchronized to the Start of Integration or External Trigger Input The Continuous Strobe is only active if the Lamp Enable command is active 225 00000 000 11 201505b 57 Ocean Corr 6 Technical Specifications Synchronizing Strobe Events If the application requires more than one pulse per integration period the user needs to insure the continuous strobe and integration period are synchronized The integration time must be set so that an equal number of strobe events occurs during any given integration period
50. The Flame has the ability to function as a SPI master through the SPI port which comprises the SPI Master Clock SPI Master MOSI SPI Master CS and SPI Master MISO pins To send messages over the SPI port use the General SPI Input Output message The Flame does not send or receive any SPI data without direction from its host PC Because SPI is a full duplex transaction the Genera SPI Input Output message both reads and writes at the same time For instance a four byte write will return four bytes of dummy read data and a four byte read requires four bytes of dummy write data e MOSI data is established just prior to the rising edge of the SPI clock e MISO data is sampled just after a falling edge of the SPI clock DC The Flame has the ability to function as an I2C master through the I2C port which comprises the I2C SDA and I2C SCL pins To send messages over the I2C port use the General I2C Write and General I2C Read messages Note that the Flame does not send or receive any I2C data without direction from its host PC The I2C lines are pulled up internally to 3 3V by 2K resistors Performance Charts The following show some performance characteristics of the Flame S 52 225 00000 000 11 201505b Qosen gt Optics 6 Technical Specifications Dark Current vs Temperature Counts per ms 10 o 10 20 30 40 50 Temperature C This is a plot of the dark current in counts per millisecond versus temperature
51. XR1 ES FLAME T RAD 360 1000 Irradiance For more information and specifications on preconfigured models see www oceanoptics com 200 1025 225 00000 000 11 201505b 7 Ocean Crm 1 Introduction 8 225 00000 000 11 201505b Chapter 2 Installation and Setup What s In the Box a Packing List The packing list is inside a plastic bag attached to the outside of the shipment box the invoice arrives separately It lists all items in the order including customized components in the spectrometer such as the grating detector collection lens and slit The packing list also includes the shipping and billing addresses as well as any items on back order a Flame Spectrometer Your Flame spectrometer arrives pre calibrated and ready to plug and play a Micro USB Cable Use this cable CBL MICROTOA USB to connect your spectrometer to a computer running on a Windows Mac or Linux operating system a Wavelength Calibration Data Sheet Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information unique to your spectrometer OceanView reads this calibration data from your spectrometer when it interfaces to a computer via the USB port Note Please save the Wavelength Calibration Data Sheet for future reference Flame Installation The following procedure provides general instructions for getting your new Flame spectrometer up and running 225 00000 000 11 201505b
52. ZeInstalation and Sep AECH irradiance Irradiance is the technique of measuring the total energy of the light vs wavelength either relative to the output of a known source relative irradiance or in absolute units of power or energy absolute irradiance This is used widely in light metrology color measurement and environmental science Absolute irradiance measurements require an irradiance calibrated spectrometer This can be done in the factory for fixed configurations or by using a calibration lamp in the lab or field Every time a set up is changed it must be recalibrated Computer Flame Spectrometer LED Power Supply 400 um Fiber Integrating Sphere Typical Relative Irradiance Set up for Measuring Light Power Output of an LED Using and Integrating Sphere Flame Spectrometer Computer H 400 um Premium grade Patch Cord Radiometrically Calibrated Light Source Cosine Corrector Ray Typical Set up for an Absolute Irradiance Measurement Using Field Calibration with a Calibrated Light Source 225 00000 000 11 201505b Kocen ip Optics 2 Installation and Setup Common Irradiance Applications e Measuring the radiant output of lamps and LEDs e Measuring color using relative irradiance e Measuring the color rendering index CRI e Measuring UV exposure for health and safety 225 00000 000 11 201505b 21 Ocean ZeInstalation nd Setup AE E 22 225 00000 000 11 201505b Chapter 3 Flame Op
53. a measurement significantly 225 00000 000 11 201505b 25 Ocean Sem e Boxcar Boxcar is a form of averaging across pixels It applies a rolling average to multiple adjacent pixels to help smooth the spectral response and reduce the impact of noise e Electric Dark Correction on off There are pixels on the detector that are kept deliberately dark Dark correction subtracts the signal from these dark pixels to reduce the impact of thermal noise which produces a baseline signal from the detector e Non Linearity Correction on off Detectors do not have a completely linear response As they approach saturation typically their efficiency reduces e Stray Light Correction An advanced user option that allows you to set a 1 or 2 term polynomial correction for stray light correction e Trigger Modes Sets triggering mode For more information on triggering see External Triggering e Strobe Lamp on off Use this function to turn an attached light source on or off e GPIO Controls Can be used to control compatible accessories or custom hardware Can be set to three states on off and alternate Controls that appear in this window depend on the spectrometer model You can add and remove acquisition controls from this window 3 Operation Quick View and Device Response In Quick View mode formerly called Scope mode the spectra that are displayed have an arbitrary y axis given in counts This is the raw signal f
54. a E 67 USB Information sitiseni da aa tt a dt at ca a aie baia 67 lge ei BT 67 Command Synhtax tess cs otita t a n tt at ei Meena aa i la a a 67 USB Command SUMMA Y esiisa aa da anda a ta ta aaa la a 68 USB Command Descriptions mmm nea aaa amana aaa ana ana aaa 69 FLAME S Serial Port Interface Communications and Control Information mcce anna 83 Hardware Description sac cae ie e a at i it n it la a a e it a e ta it i 83 Instruction Set osii aere aaa e n aaa a a a d i e n ao a i ba 83 Command Descriptions men eee eee na ame eannaa aaa ane e Kassadan naadik narani 85 FLAME T Firmware aia pa a ala a ac 93 Hardware Description msi ceea e ea c ca bea ia c i a a aa al i a a a a a lees 93 Spectral Memory Storage cccecececeeeceeeeeeeeeeeceeeeeeeaeeeeeaeeeeeeecaeeeeaaeegeaeeseeeeeseaeeesaaeseeaeeseeeeess 93 lhstruction Set oc 3sadenascet desea oua ai aut ab ai a a pia al i i ta i n ta al a ne al i 93 Command Syntax TEE 93 225 00000 000 11 201505b iii Kocen Table of Contents Optics USB Command Gummar nenea amana ana nea aaa amana aaa ana ana 94 USB Command Descriptions mmm nea aaa amana ana ana aaa 94 FLAME T Serial Port Interface Communications and Control Information 107 Leite VEER le en DE 107 Spectral Memory Storage cita acte a a ct i a st a a la ta 107 Instruction Set scie sineta et a tt a a i tt a a ul ala ac 107 Command SUMmMary x o carta i a a ta a i a oa a i a ta o t a 1
55. a ana aaa 26 Continuous and Single Acquisitions mmm ana nn aaa ana na 26 Projects and Methods eco sinea inca atata au tea ata aa ata i ta Dc a a a a ata a da 28 Spectroscopy Application Wizards nenea enma ana nn aaa ana aaa 28 Dark and Reference Measurement ee eee eee enma ana ana enma ee ana 29 Schematic View tau nioo ot d a dat na a a d a a i ia aaae 29 Chapter 4 Troubleshooting m mun eeeeeeeeeene nene eeeenneneeaena aan 31 OVE WY e ni tt e i e ia a main a a ai a e aa e 31 Microsoft Windows Operating Systems nenea nea nneenaeaaa nnaae anaeaae 32 Apple Mac Operating Systems nenea nenea aaa amana aaa ana ana 33 Linux Operating Systems atita ao eae a at a e e Cr ns a ba ali 33 Chapter 5 How the Flame Spectrometer Works ccccseeseeeeeees 37 CEI OU ant a la ia a la ia na asa il t and aa ai ea na 37 Chapter 6 Technical Specifications ceseeeeeeeeeeeeeeeeeeeeeeeees 45 M c hanical eer 48 ISG ir iCal PiU e 49 DD4 Accessory Connector Pinout Diagram nenea nea ana nea nau ana aaa 49 SEL ert aaa aa da at ap ai a an ad d el tt a i a aan ba datat 52 Eesen Bedeelegte a d a i nad i a SA a la an adi d ia 52 IER AV IEN se ai acea aa one a au al nea ia oa Aaaa lime le el 52 CCD RTE 54 CCD Well Depth 2c ici acea a Be e nai aaa a a a a a a a e a e a deeded 55 ii 225 00000 000 11 201505b Koco Opt ICs Table of Conten
56. ates a spectral acquisition The Flame will acquire a complete spectrum 3840 pixel values The data is returned in bulk transfer mode through EP2 and EP6 depending on the USB Communication Speed The table below provides the pixel order overview for the 2 different speeds The pixel values are decoded as described below Byte Format Byte 0 0x09 Return Format The format for the returned spectral data is dependent upon the USB communication speed The format for both High Speed 480 Mbps and Full Speed 12Mbps is shown below All pixel values are 16 bit values which are organized in LSB MSB order There is an additional packet containing one value that is used as a flag to insure proper synchronization between the PC and Flame USB High Speed 480Mbps Packet Format In this mode the first 2K worth of data is read from EP6In and the rest is read from EP2In The packet format is described below 225 00000 000 11 201505b 97 Kocen 8 Firmware and Advanced Communications bor ICs Packet End Point Bytes Pixels 0 EP6In 512 0 255 1 EP6In 512 256 511 2 EP6In 512 512 767 3 EP6In 512 768 1023 4 EP2In 512 1024 1279 5 EP2In 512 1280 1535 EP2In 512 14 EP2In 512 3584 3840 15 EP2ln 1 Sync Packet The format for the first packet is as follows all other packets except the synch packet has a similar format except the pixel numbers are incremented by 256 pixels for each packe
57. ation time in us LSW MSW Within each word order is LSB MSB 6 Lamp Enable 0 Signal LOW 1 Signal HIGH 82 225 00000 000 11 201505b Ocean port ICS 8 Firmware and Advanced Communications Byte Description Comments 7 Trigger Mode Value 8 Spectral Acquisition Status 9 Packets In Spectra Returns the number of Packets in a Request Spectra Command 10 Power Down Flag 0 Circuit is powered down 1 Circuit is powered up 11 Packet Count Number of packets loaded into End Point Memory 12 Reserved 13 Reserved USB Communications 0 Full Speed 12Mbs Speed 0x80 High Speed 480 Mbps 15 Reserved FLAME S Serial Port Interface Communications and Control Information The Flame is a microcontroller based Miniature Fiber Optic which can communicate via the Universal Serial Bus or RS 232 This document contains the necessary command information for controlling the Flame via the RS 232 interface Hardware Description The Flame utilizes a Cypress FX2 microcontroller which has a high speed 8051 combined with an USB ASIC Program code and data coefficients are stored in external E7PROM which are loaded at boot up via the I C bus Instruction Set Command Syntax The list of the commands is shown in the following table along with the microcode version number they were introduced with All commands consist of an ASCII character passed over the seria
58. changed Start saving data Turns red when save is active If saving data continuously click when red E to stop saving Will only activate saving for acquisitions attached to that particular view m AD Global Save Activates all configured saves across all views Use to save data from multiple T spectrometers at the same time By default OceanView will save data as a single snapshot acquisition By configuring the save you can set the save behavior to fit your measurement needs from single snapshots to a continuous stream of data over time Saved Data Panel The saved data panel lets you see your data as it is saved and preview data It also makes it simple to add overlays of saved data to your active view B Views Bi View Minus Bkgnd_7 pioj i aeeoOo n lt oaPPRPaAaRe SP IB A de 0 Intensity counts T E Wavelength nm Saved Data oe C Users henry langston Desktop OV Testing 1 453 i Header Timestamp Frame oceanview V Auto scale use as over Autoset integration time false a mo Trigger mode 0 Integration Time sec 1 00000 on Bai Scans to average 10 N File name Creation Date Electric dark correction enabled zm pf N use4co22371_15 27 12 399 0e 02 Feb 2015 15 27 12 400 lt i ees f VA lusB40022371_15 27 36 999 be 102 Feb 2015 15 27 37 009 3 Na usesco22371_15 27 38 994 b 2015 15 27 39 004 mm pi wm use4c022371_15 2
59. chronization 3 External Hardware Trigger Response ACK or NAK Default value 0 ASCII Data Mode Sets the mode in which data values are interpreted to be ASCII Only unsigned integer values 0 65535 are allowed in this mode and the data values are terminated with a carriage return ASCII 13 or linefeed ASCII 10 In this mode the Flame echoes the command and data values back out the RS 232 port Command Syntax aA Response ACK or NAK Default value N A Note The command requires that the string aA be sent without any CR or LF This is an attempt to insure that this mode is not entered inadvertently A legible response to the Version number query v command indicates the Flame is in the ASCII data mode 225 00000 000 11 201505b 113 8 Firmware and Advanced Communications Binary Data Mode Ocean Sm Sets the mode in which data values are interpreted to be binary Only 16 bit unsigned integer values 0 65535 are allowed in this mode with the MSB followed by the LSB Command Syntax bB Response ACK or NAK Default value Default at power up not changed by Q command Note The command requires that the string bB be sent without any CR or LF This is an attempt to insure that this mode is not entered inadvertently Checksum Mode Specifies whether the Flame will generate and transmit a 16 bit checksu
60. cident number of photons to a calibration This can be either absolute an atomic emission light source of known output power or relative corrected for instrument response function but not absolute units It can be considered a measurement technique and is used widely in remote sensing light metrology and anywhere where you wish to characterize the incident light source Irradiance calibrations are not required for many techniques because these measure the relative signal changes with respect to the sample and not the light source You can find out more about irradiance calibration techniques at http oceanoptics com measurementtechnique irradiance OceanView has wizards that will step you through absolute irradiance and relative calibrations and more information on these is located in the OceanView manual see Product Related Documentation 225 00000 000 11 201505b 65 Ocean Crm 7 Calibration 66 225 00000 000 11 201505b Chapter 8 Firmware and Advanced Communications FLAME S Firmware The Flame is a microcontroller based Miniature Fiber Optic Spectrometer that can communicate via the Universal Serial Bus or RS 232 This section contains the necessary command information for controlling the Flame via the USB interface command This information is mainly relevant to those who dont wish to use Ocean Optics OmniDriver or SeaBreeze device drivers or to those who wish to communicate via RS 232 Hardware Description The Flame
61. click on the Unknown Device listing and select the Uninstall or Remove option 4 Click the OK button to continue A warning box appears confirming the removal of the Unknown Device Click the OK button to confirm the device removal 5 Disconnect the Flame from your computer 6 Replug the spectrometer into your computer The system should now able to locate and install the correct drivers for the USB device Apple Mac OSX Operating Systems Since there are no device files for the Flame Spectrometer in a Mac operating system you should not encounter any problems if you installed the spectrometer before the spectrometer operating software Linux Operating Systems For Linux operating systems all you need to do is install the spectrometer operating software then unplug and replug in the spectrometer Technically the driver files for Linux simply give nonprivileged users permission to use newly connected hardware There isn t any long term harm to plugging in the device before installing the software have both SpectraSuite and OceanView installed Will my spectrometer work with both Yes There should be no driver issues with Windows 64 bit Mac and Linux operating systems since they all use the winusb driver For Windows 32 bit systems you will need to swap the drivers when switching software since OceanView uses the winusb driver while SpectraSuite uses the ezusb driver 225 00000 000 11 201505b 4 Troubleshooting Ocean
62. d and Probe Holder Common UV Vis Reflectance Applications e Diffuse and Specular Color Measurements e Process control for Surface quality of metals e Thin film and semiconductor metrology Common UV Vis Transmission Applications e Turbidity measurements of chemical solutions e Measuring the transmission efficiency of optics and glass 18 225 00000 000 11 201505b Ocean We Optics Fluorescence Fluorescence is a technique where a sample is excited with a light source and 2 Installation and Setup fluorescent light emitted from the sample at a higher wavelength is measured by the spectrometer Typically the excitation source is applied at 90 to the sample to minimize light from the excitation source reaching the spectrometer Similarly filters are used to block lower wavelength light from reaching the detector Spectrometers used for fluorescence typically have a large slit sacrificing resolution for throughput sensitivity gt CVFL Q 10 Cuvette with Sample Solution Computer Flame Spectrometer PX2 Lamp QP1000 2 UV VIS QP600 1 SR CUV All Typical Fluorescence Set Up with an LED Excitation Source at 900 QR400 7 VIS NIR Common Fluorescence Applications e Identifying proteins using fluorophores NADH fluorescence Remote sensing of chlorophyll Medical diagnosis of tumors and tissue types Detection of anti counterfeiting tags 225 00000 000 11 201505b 19 Ocean
63. d for gt 400 nm 225 00000 000 11 201505b 43 2Ocean n the Flame Spectrometer Works OPTICS 44 225 00000 000 11 201505b Chapter 6 Technical Specifications Specification FLAME S FLAME T Optical and Spectroscopic Integration Time 1 ms 65 seconds 3 8 ms to 10 seconds Dynamic Range for single acquisition 1300 1 Dynamic Range of system 2 x 10 3 4 x 10 Signal to Noise single acquisition 250 1 300 1 Resolution FWHM 0 1 10 0 nm configuration dependent Stray Light lt 0 05 at 600 nm lt 0 10 at 435 nm Scan rate max 400 Hz 260 Hz Spectrometer Channels One Thermal Stability 0 02 nm C for 650 nm range 0 06 pixels C Triggering 4 modes Triggering Jitter 21 nanoseconds Detector Type Sony ILX511B CCD Toshiba TCD1304AP CCD Detector range 190 1100 nm Pixels 2048 pixels 3648 pixels Pixel size 14 um x 200 um 8 um x 200 um Electronic shutter No Yes Pixel well depth 62 500 electrons 100 000 electrons Readout noise single dark spectrum 50 counts RMS 300 counts peak to peak 225 00000 000 11 201505b 45 6 Technical Specifications Ocean ES Ics Specification FLAME S FLAME T Corrected linearity gt 99 8 Filters optional 2 and 3 order rejection long pass Electrical Power requirement spectrometer functions
64. d the data is retrieved through End Point 1 In When using Query Information to read EEPROM slots data is returned as ASCII text However everything after the first byte that is equal to numerical zero will be returned as garbage and should be ignored The 20 configuration variables are indexed as follows Configuration Index Description 0 Serial Number 1 0 order Wavelength Calibration Coefficient 2 1 order Wavelength Calibration Coefficient 3 2 order Wavelength Calibration Coefficient 4 3 order Wavelength Calibration Coefficient 5 Stray light constant 6 0 order non linearity correction coefficient 7 1 order non linearity correction coefficient 8 2 order non linearity correction coefficient 9 3 order non linearity correction coefficient 10 4 order non linearity correction coefficient 11 5 order non linearity correction coefficient 12 6 order non linearity correction coefficient 13 7 order non linearity correction coefficient 14 Polynomial order of non linearity calibration 15 Optical bench configuration gg fff sss gg Grating fff filter wavelength sss slit size 16 Flame configuration AWL V A Array coating Mfg W Array wavelength VIS UV OFLV L L2 lens installed V CPLD Version 17 Reserved 18 Reserved 19 Reserved 225 00000 000 11 201505b 71 Kocen 8 Firmware and Advanced Communications
65. e Trigger 0x30 Reserved 225 00000 000 11 201505b 103 Kocen 8 Firmware and Advanced Communications Opt ics i Time Base Register E zg Max Value Description alue alue Address Single Strobe High 0x38 Clock Transition 1 0 OxFFFF 2MHz Delay Count Single Strobe Low 0x3C Clock Transition 5 0 OxFFFF 2MHz Delay Count 0x40 Lamp Enable 0 0 1 N A GPIO Mux Register 0x48 or pinis GE pin 0 0 oxo3FF N A 1 pin is alternate function GPIO Output Enable 0x50 1 pin is output 0 0 Ox03FF N A O pin is input GPIO Data Register For Output Write 0x54 value of signal 0 0 Ox03FF N A For Input Read current GPIO state 0x58 Reserved 0x5C Reserved 0x74 Offset Value 0 0 OxFFFF N A Offset Control Bit 0 Enable Auto Nulli 0x78 ai 0 0 OxFFFF N A Bit 1 Enable Auto Nulling Saturation Ox7C FE GA Piograri ie Geo N A N A N A Read Only 0x80 oe saturation per Ja OxFFFF N A 104 Notes User should not change these values because spectrometer performance can be affected This information is included just for completeness amp These values are controlled by other command interfaces to the Flame i e Set integration time command 225 00000 000 11 201505b Koco Opt ICs 8 Firmware and Advanced Communications Read Register Information Reads the values from any of the registers above This command is sent to End Po
66. e broad The traditional boxcar algorithm averages n pixel values on each side of a given pixel 6 Technical Specifications Time based and spatial based algorithms are not correlated so therefore the improvement in S N is the product of the two processes In review large well devices are far less sensitive than small well devices and thus require a longer integration time for the same output Large well devices achieve a good S N because they integrate out photon noise Small well devices must use mathematical signal averaging to achieve the same results as large well devices but small well devices can achieve the results in the same period of time This kind of signal averaging was not possible in the past because analog to digital converters and computers were too slow Large well devices consume large amounts of power resulting in the need to build thermoelectric coolers to control temperature and reduce electronic noise Then even more power is required for the temperature stabilization hardware But small well devices only need to use signal averaging to achieve the same results as large well devices and have the advantages of remaining cool and less noisy Internal Operation Pixel Definition A series of pixels in the beginning of the scan have been covered with an opaque material to compensate for thermal induced drift of the baseline signal As the Flame warms up the baseline signal will shift slowly downward a few counts depend
67. e responds with ACK at new baud rate otherwise it responds with NAK and old baud rate is used Notes If a deviation occurs at any step the previous baud rate is used The power up Baud rate can be set by setting the EEPROM Memory slot to the desired value i e 6 for a value of 115 200 Baud Pixel Mode Specifies which pixels are transmitted While all pixels are acquired on every scan this parameter determines which pixels will be transmitted out the serial port 225 00000 000 11 201505b 111 Ocean Som 8 Firmware and Advanced Communications Command Syntax P DATA WORD Description Example 0 all 3870 pixels P O spaces for clarity 1 every n pixel with no averaging only 2 N A P 1 lt Enter gt 3 pixel x through y every n pixels N lt Enter gt 4 up to 10 randomly selected pixels P2 N A between 0 and 2047 denoted p1 p2 P3 lt Enter gt p10 x lt Enter gt Value y lt Enter gt n lt Enter gt P 4 lt Enter gt n lt Enter gt pi lt Enter gt p2 lt Enter gt p3 lt Enter gt p10 lt Enter gt Response ACK or NAK Default value 0 Note Since most applications only require a subset of the spectrum this mode can greatly reduce the amount of time required to transmit a spectrum while still providing all of the desired data This mode is helpful when interfacing to PLCs or other processing equipment Spectral Acquisition Acquires spectra with the current set of
68. ell device that integrates to saturation at one short At and then signal average mathematically n times Theoretically both approaches achieve the same results though there are large differences in actual operation Traditional spectroscopic instruments use large well devices and 16 bit ADCs to achieve the defined S N The Flame uses a small well device and utilizes signal averaging to achieve the same S N A brief comparison of large and small well devices is shown below Well Depth Comparison Large well CCDs Small well CCDs Low photon noise Medium photon noise that can be averaged out Low optical sensitivity High optical sensitivity High power consumption Low power consumption gt 10 MHz operating speeds Moderate operating speeds 2 MHz Signal Averaging Signal averaging is an important tool in the measurement of spectral structures It increases the S N and the amplitude resolution of a set of samples The types of signal averaging available in our software are time based and spatial based When using the time base type of signal averaging the S N increases by the square root of the number of samples Signal averaging by summing is used when spectra are fairly stable over the sample period Thus a S N of 2500 1 is readily achieved by averaging 100 spectra 225 00000 000 11 201505b 55 Ocean Sm Spatial averaging or pixel boxcar averaging can be used to improve S N when observed spectral structures ar
69. enerate and transmit a 16 bit checksum of the spectral data This checksum can be used to test the validity of the spectral data and its use is recommended when reliable data scans are required Command Syntax k DATA WORD 0 Do not transmit checksum value Value 10 transmit checksum value at end of scan Response ACK or NAK Default value 0 Version Number Query Returns the version number of the code running on the microcontroller A returned value of 1000 is interpreted as 1 00 0 Command Syntax v Response ACK followed by DATA WORD Default value N A 225 00000 000 11 201505b 91 Ocean Sm 8 Firmware and Advanced Communications Calibration Constants Writes one of the 16 possible calibration constant to EEPROM The calibration constant is specified by the first DATA WORD which follows the x The calibration constant is stored as an ASCII string with a max length of 15 characters The string does not check to see if it makes sense Command Syntax X DATA WORD ASCIl STRING DATA WORD Index description 0 Serial Number 1 0 order Wavelength Calibration Coefficient 2 1 order Wavelength Calibration Coefficient 3 2 order Wavelength Calibration Coefficient 4 3 order Wavelength Calibration Coefficient 5 Stray light constant 6 0 order non linearity correction coefficient 7 1 order non linearity correction coefficient 8
70. eration with OceanView Overview The following information enables you to perform the basics of acquiring and saving data with your Flame Spectrometer and OceanView software More detailed information about OceanView is in the OceanView Manual see Product Related Documentation Launch OceanView Once you have installed your software and connected your spectrometer you are ready to display your measurement data using OceanView Launching OceanView differs depending on your operating system and where you have placed your OceanView program files For PCs running Microsoft Windows the default location is Start Programs Ocean Optics OceanView OceanView For Mac computers the default location is the Applications folder When you first start OceanView the Welcome screen appears Welcome Screen xi Quick Load a Spectroscopy View Saved Project Application Wizards Restore Last Session OceanView Welcome Screen Version 1 5 Select from the following tasks e Quick View Displays the spectrum in Quick View mode showing raw unprocessed data This is uncorrected for instrument response vs wavelength Quick View shows you a live shot of what the spectrometer is seeing From Quick View you can launch application wizards or construct your own method 225 00000 000 11 201505b 23 Ocean Ear e Load a Saved Project Loads a previously saved project Click Restore Last Session to reload the schematic and views a
71. ess that is passed as the second byte of the LC write command is this 7 bit address which will be shifted 1 bit left and appended with the R W bit to form the first byte of the DC write transaction The DC bus runs at 400KHz The results codes are described above Command Byte Format Byte 0 Byte 1 Byte 2 Byte 3 wii Byte N 3 0 DC Address Bytes to Data ByteO Data byte N x61 Write Return Byte Format Byte 0 CC Results 225 00000 000 11 201505b 77 Ocean Sem 8 Firmware and Advanced Communications General SPI Input Output Performs a general purpose write and read on the SPI bus for interfacing to attached peripherals The time to complete the command is determined by the amount of data transferred and the response time of the peripheral Wait at least 10 ms after sending a Write command before reading the Return value The SPI bus runs at 125KHz Clock The maximum number of bytes that can be written or read is 61 During this transfer the SPI Chip Select signal is driven to an active LOW TTL level Data is transmitted out the MOSI Master Out Slave In line on the rising edge of the clock signal Data is also latched in the from the MISO line on the falling edge of the clock signal Command Byte Format Byte 0 Byte 1 Byte 2 Byte 3 ise Byte N 2 0 of Bytes N Write ByteO Write Byte 1 ba Write Byte x62 N Return Byte Format Byte 0 Byte
72. events the spectrometer auxiliary power from drawing excessive current from the USB power supply Devices drawing power from this pin must comply with USB specifications especially that the total power draw of the spectrometer and the user s device must not exceed 500mA Therefore it is recommended that devices draw less than 100mA from this pin as future Flame spectrometer variants may internally require up to 400mA Auxiliary Power 40 In Out Input This pin can also be used to supply power to the Flame when 5V USB is not present In this case because the pin is connected downstream of the load switch the soft start and overcurrrent protection have been bypassed For future compatibility it is recommended that any peripherals supplying current into this pin must be able to supply at least 500mA 225 00000 000 11 201505b 51 Ocean Sm 6 Technical Specifications Voltage Level Description Pin Function Note Do not supply both USB input power and auxiliary input power concurrently If both supplies are connected the result will be a load share Although the load switch should protect the two supplies from contention if they are connected the performance of the spectrometer may be affected See the Caution below Caution Do not connect the GPIO pins to 5V The GPIOs are not 5V tolerant and will be damaged if connected to 5V The maximum voltage is 4V SPI
73. f signal 0 0 0x03FF N A For Input Read current GPIO state 0x58 Reserved 0x5C Offset Value 0 0 OxFFFF N A Offset Control 0x60 Bit 0 Enable Auto Nulling 0 0 OxFFFF N A Bit 1 Enable Auto Nulling Saturation 0x64 FPGA Programmed Read Only 0x5501 N A N A N A 0x68 Maximum Saturation Level Ox55FO 0 OxFFFF N A Notes User should not change these values because spectrometer performance can be affected This information is included just for completeness amp These values are controlled by other command interfaces to the Flame i e Set integration time command 80 225 00000 000 11 201505b Kocen pori ICS 8 Firmware and Advanced Communications Read Register Information Read the values from any of the registers above This command is sent to End Point 1 Out and the data is retrieved through End Point 1 In Byte Format Byte 0 Byte 1 Ox6B Register Value Return Format EP1In Byte 0 Byte 1 Byte 2 Register Value Value LSB Value MSB Read PCB Temperature Read the Printed Circuit Board Temperature The Flame contains a DS1721 temperature sensor chip which is mounted to the underside of the PCB This command is sent to End Point 1 Out and the data is retrieved through End Point 1 In The value returned is a signed 16 bit A D conversion value which is equated to temperature by Temperature C 003906 ADC Value Byte Format
74. f the detectable light level and can be thought of as the maximum detectable light level at the minimum integration time divided by the minimum detectable non real time operating system 3 Scan rate is dependent on the operating computer and not the spectrometer These figures assume a Contact info oceanoptics com to obtain copies of certifications 225 00000 000 11 201505b 47 Mechanical Diagram GENERAL NOTES UNLESS NOTED OTHERWISE 1 All dimensions subject to change without notice 2 For STEP file see 225 00000 00_CD step 3X 4 40 X 7 30 MOUNTING HOLES Figure 1 Flame Outer Dimensions 48 225 00000 000 11 201505b Koco ip Optics 6 Technical Specifications Electrical Pinout The Flame features a 40 pin Accessory Connector located on the front of the unit as shown Aflame C Ocean Miniature Spectrometer DD4 Connector Slit Location of Flame Accessory Connector DD4 Accessory Connector Pinout Diagram When facing the 40 pin Accessory Connector on the front of the vertical wall of the Flame pin number 1 is on the right Listed below is the pin description for the Flame Accessory Connector located on the front vertical wall of the unit The Flame will include a JAE DD4 receptacle part number DD4RA40JA1 Most accessories that plug into the Flame will include a JAE DD4 plug part number DD4PA40MA1 There is also a vertical connector JAE part number DD4BA40WA1
75. h a 20 150ms integration time set number of scan to add to 5 and operate at 115 200 Baud aA Set ASCII Data Mode gt K6 lt CR gt Start baud rate change to 115 200 Wait for ACK change to 115200 wait for 20ms gt K6 lt CR gt Verify command communicate at 115200 gt A5 lt CR gt Add 5 spectra gt i20150 lt CR gt Set integration time to 20 150ms S Acquire spectra Repeat as necessary Application Tips e During the software development phase of a project the operating parameters of the Flame may become out of synch with the controlling program It is good practice to cycle power on the Flame when errors occur e If you question the state of the Flame you can transmit a space or another non command using a terminal emulator If you receive a NAK the Flame is awaiting a command otherwise it is still completing the previous command e Use a terminal emulator and configure the following port settings a Bits per second Baud rate Set to desired rate b Data bits 8 Cc Parity None 225 00000 000 11 201505b 117 Ocean e Firmware and Advanced Communications AE ICH d Stop bits 1 e Flow control None 118 225 00000 000 11 201505b A absorbance 17 accessories 14 17 accessory connector diagram 49 acquisition continuous 26 single 26 Acquisition Parameters 25 application wizards 28 applications 3 breakout box 16 C cables DB15 15 PAK50 16 calibration 61 irradiance 65 prepa
76. he integration counter has a resolution of ims Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Byte 4 LSW LSW MSB MSW LSB MSW MSB 0x02 LSB MSW amp LSW Most Least Significant Word MSB amp LSB Most Least Significant Byte Set Strobe Enable Status Sets the Flame Lamp Enable line J2 pin 4 as follows The Single Strobe and Continuous Strobe signals are enabled disabled by this Lamp Enable Signal Data Byte 0 gt Lamp Enable Low Off Data Byte 1 3 Lamp Enable HIGH On Byte Format Byte 0 Byte 1 Byte 2 0x03 Data byte LSB Data Byte MSB Set Shutdown Mode Sets the Flame shutdown mode When shutdown the internal FX2 microcontroller is continuously running however all other functionality is disabled In this power down mode the current consumption is reduced to 250mA operating current for the FX2 microcontroller When shutdown is active active low the external 5V signal V5_Switched pin 3 is disabled in addition to all other signals except DC lines Data Byte 0 gt Shutdown everything but the FX2 Data Byte 0 3 Power up entire Spectrometer 70 225 00000 000 11 201505b ocem WP Optics 8 Firmware and Advanced Communications Byte Format Byte 0 Byte 1 Byte 2 0x04 Data byte LSB Data Byte MSB Query Information Queries any of the 20 stored spectrometer configuration variables The Query command is sent to End Point 1 Out an
77. ics accessories that use a DB 15HD connector These include the PX 2 LLS and HL 2000 FHSA light sources 225 00000 000 11 201505b 5 1 15 11 DD4 DB15 Pin Connections me Lamp Enable Reserved NC GPIO 4 jam oo C ee rate C 15 Ocean ES ics 2 Installation and Setup PAK50 Connector Cable FLAME CBL DD4P PAK50P This cable connects the Flame to the Breakout Box DD4P to PAK50P 30 Pin Connections PAK50P DD4 Name PAK50P DD4 Name 1 25 RS232 Rx 16 11 GPIO 4 2 24 RS232 Tx 17 4 Single Strobe 3 8 GPIO 2 18 12 GPIO 5 4 40 VUSB 19 16 SPI CLK 5 1 GND 20 3 Continuous Strobe 6 21 I2C SCL 21 18 SPI CS 7 6 GPIO 0 22 13 GPIO 6 8 22 I2C SDA 23 28 Reserved do not connect 9 7 GPIO 1 24 NC Reserved NC 10 2 External Trigger In 25 5 Lamp Enable 11 9 GPIO 3 26 14 GPIO 7 12 40 VUSB 27 10 GND 13 17 SPI MOSI 28 NC Reserved NC 14 40 VUSB 29 15 GND 15 19 SPI MISO 30 NC Reserved NC For the DD4 connect shield to connector case if shield is included Breakout Box HR4 BREAKOUT The Breakout Box is a passive module that separates the signals from the Flame s DD4 40 pin connector to an array of standard connectors and headers enabling functionality with a wide range of accessories In addition to the accessory connector the breakout box features a circuit board based on a neutral breadboard pattern that allows custom circuitry to be protot
78. ing on the external environment The baseline signal is set at the time of manufacture If the baseline signal is manually adjusted it should be left high enough to allow for system drift The following is a description of all of the pixels both as they exist on the hardware device and as they are actually read from the device via USB Pixels on the FLAME S Pixels on the FLAME T Pixel Type Description Pixel Type Description 1 2 Discard Not valid 1 2 Discard Not valid 3 4 Dummy Not sensitive to thermal 3 4 Dummy Not sensitive to thermal current or light current or light 5 22 Optical Sensitive to thermal 5 17 Optical Mask Sensitive to thermal current mask current but masked but masked from light from light 23 25 Bevel Partially sensitive to light 18 20 Bevel Partially sensitive to light 26 2048 Optical Sensitive to light 21 3648 Optical Active Sensitive to light Active RE CEI RET SE Beer E S gt cen EE may gt 3648 Discard Not visible in Ocean View E AAIYE ayy KOL but may be present in direct A i ON USB or RS232 Discard OceanView copies pixel 3 into pixels 1 and 2 56 225 00000 000 11 201505b Koco WP Opi ICs 6 Technical Specifications CCD Detector Reset Operation At the start of each integration period the detector transfers the signal from each pixel to the readout registers and resets the pixels The total amount of time required to perform this operation is 8
79. int 1 Out and the data is retrieved through End Point 1 In Byte Format Byte 0 Byte 1 0x6B pal Return Format EP1In Byte 0 Byte 1 Byte 2 Register Value Value MSB Value LSB Read PCB Temperature Reads the Printed Circuit Board temperature The Flame contains a DS1721 temperature sensor chip which is mounted to the underside of the PCB This command is sent to End Point 1 Out and the data is retrieved through End Point 1 In The value returned is a signed 16 bit A D conversion value which is equated to temperature by Temperature C 003906 ADC Value Byte Format Byte 0 Ox6C Return Format EP1In Byte 0 Byte 1 Byte 2 Read Result ADC Value LSB ADC Value MSB If the operation was successful the Read Result byte value will be 0x08 All other values indicate the operation was unsuccessful Read Irradiance Factors Reads 60 bytes of data which is utilized for Irradiance Calibration information from the desired EEPROM memory address 225 00000 000 11 201505b 105 8 Firmware and Advanced Communications Ocean Sem Byte Format Byte 0 Byte 1 Byte 2 Ox6D EEPROM Address LSB EEPROM Address MSB Return Byte Format Byte 0 Byte 1 Byte 59 Byte 0 Byte 1 Byte 59 Write Irradiance Factors Write 60 bytes of data which is used for Irradiance Calibration information to the desired EEPROM
80. it Sets the Flame s integration time in microseconds to the value specified Command Syntax i 32 bit DATA DWORD Response ACK or NAK Range 10 65 000 000 Default value 6 000 Lamp Enable Sets the Flame s Lamp Enable line to the value specified Command Syntax J DATA WORD Value O Light source strobe off Lamp Enable low 1 Light source strobe on Lamp Enable high Response ACK or NAK Default value 0 Baud Rate Sets the Flame s baud rate 110 225 00000 000 11 201505b OCE pop Ics 8 Firmware and Advanced Communications Command Syntax K DATA WORD Value 0 2400 1 4800 2 9600 3 19200 4 38400 5 Not Supported 6 115 200 7 230 400 Response See below Default value 2 When changing baud rates the following sequence must be followed 1 Controlling program sends K with desired baud rate communicating at the old baud rate 2 The Flame responds with ACK at old baud rate otherwise it responds with NAK and the process is aborted 3 Controlling program waits longer than 50 milliseconds 4 Controlling program sends K followed by the desired baud rate For example to change the baud rate from 9600 to 115 200 in ASCII mode e At 9600 baud send K6 lt enter gt You will receive an ACK at 9600 baud e Send K6 lt enter gt at 115200 Note that the K is not echoed back but the 6 is e You will receive two ACK characters and the prompt at 115200 baud 5 The Flam
81. it of Liability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this manual Table of Contents About This Manual i iei cacao ci EE a Ee Vv Document Purpose and Intended Audience men eee enma emana ee ana v Document SUMIMANY e tri e beata da ada det Da a Ata a ln o a bad Ca da ca v Product Related Documentation cnnneeeee nenea ea nana ee anna ee amana vi Document Versio LEE vi Patent Pending NEIE sa once antet anii oa tt ui a n aa fn e a e vi NAV Ju e vi ISO CeriificatiOn NEE vii Chapter 1 Introduction 00 capete alai aan d du 28 lau za ala ana 1 Productintroduciioni sm scantei E ca eta aa a E a aaa ab p tale taia tata neta de 1 Product D le 2 Typical Applications i otto ot og Saab ao arabe SL bd au be ana Da SU be 3 Product Versi n ke eta ba aaa delta zebra db db dog dar ag data 6 Chapter 2 Installation and Setup cccce cecene eee eeneeeeeaeaaaae 9 What s In the BOX anna 9 Flame e GET e EE 9 Software Instalati M EE 10 ef ee EE 11 Hardware Setup iesen gege kee eege eege 11 Hardware Features in ari Ae a a a 0 a a a a al i at ad bain EE EEN 13 Flame RE 13 ER e EE 13 e ee 14 EISE Ree
82. l Triggering Options document located on our website at_http oceanoptics com wp content uploads External Triggering Options_Firmware3 0andAbove pdf The following paragraphs describe these modes Normal In the Normal Free run mode the spectrometer will acquire a spectrum based on the integration period specified through the software interface This data is made available for reading as soon as all the data is stored The spectrometer will then immediately try to acquire two additional spectra even if none have been requested If a new spectrum request has come from the user during either the second or third integration cycle then the appropriate spectrum will be available to the user If a second spectrum has not been requested then the spectrometer will not save the second or third spectrum and will go into an idle mode waiting for a new spectrum request from the user In this scenario a new acquisition begins when a new spectrum is requested No further spectra are acquired until the original spectrum is read by the user 58 225 00000 000 11 201505b Kocen Opt ICs 6 Technical Specifications External Synchronous Trigger Mode In the External Synchronous Trigger mode two external triggers are required to complete a data acquisition The first rising edge starts the integration period and the second rising edge stops the integration and starts the next Thus the integration time is the period between the two external trigger pulses
83. l port followed by some data The length of the data depends on the command The format for the data is either ASCII or binary default The ASCII mode is set with the a command and the binary mode with the b command To insure accurate communications all commands respond with an ACK ASCII 6 for an acceptable command or a NAK ASCII 21 for an unacceptable command i e data value specified out of range In the ASCII data value mode the Flame echoes the command back out the RS 232 port In binary mode all data except where noted passes as 16 bit unsigned integers WORDs with the MSB followed by the LSB By issuing the v command Version number query the data mode can be determined by viewing the response ASCII or binary 225 00000 000 11 201505b 83 8 Firmware and Advanced Communications Ocean CE ics In a typical data acquisition session the user sends commands to implement the desired spectral acquisition parameters integration time etc Then the user sends commands to acquire spectra S command with the previously set parameters If necessary the baud rate can be changed at the beginning of this sequence to speed up the data transmission process RS232 Command Summary Letter Description Set Pixel Boxcar SES Ge E Clear Memory Set Data Storage Mode Partial Pixel Mode Starts spectral acquisition with previously set parameters Sets trigger mode 84 225 00000 000 11 201505b A
84. ly appear when you start OceanView and should be acquiring with the default acquisition parameters If you do not see a signal or the Flame icon on the schematic you may need to rescan for spectrometers gt Procedure To rescan for attached devices 1 Click on the Device Manager icon E 2 Click Rescan The spectrometer should automatically connect Device Manager x EARCH NES O Flame 5 H juse o Device Actions Disconnect Add Device IV Simulate device if none found Rescan JV Automatically connect to devices Rescan Button Set Acquisition Parameters Set Acquisition parameters in the Acquisition Group Window to control the spectrometer This window may be minimized when you first start OceanView You can either expand or open a new window from the menu Window Acquisition Group An active acquisition is required for the Acquisition window to appear Functions available to control in the Acquisition window include the following e Integration Time Sets the integration time the time over which the detector captures incident light At the end of the integration time the accumulated signal is read from the detector by the electronics e Averaging Signal especially at low levels is often significantly impacted by noise Averaging several spectra together reduces the impact of noise and provides a cleaner result However at long integration times averaging can increase the total time of
85. m blue red OF1 WG305 Longpass filter transmits light gt 305 nm black white OF1 U325C Bandpass filter transmits gt 245 and lt 390 nm white green OF1 GG375 Longpass filter transmits light gt 375 nm red black OF1 GG395 Longpass filter transmits light gt 395 nm white red OF1 CGA420 Longpass filter transmits light gt 420 nm orange white OF1 GG475 Longpass filter transmits light gt 475 nm green green OF1 0G515 Longpass filter transmits light gt 515 nm pink yellow OF1 OG550 Longpass filter transmits light gt 550 nm orange orange OF1 OG590 Longpass filter transmits light gt 590 nm red pink OF1 RG695 Longpass filter transmits light gt 695 nm white blue OF1 RG830 Longpass filter transmits light gt 830 nm black blue OF1 CGA1000 Nonfluorescing longpass filter transmits gt 1000 nm red green OF1 CGA760 Nonfluorescing longpass filter transmits gt 760 nm blue black OF1 CGA780 Nonfluorescing longpass filter transmits gt 780 nm white yellow OF1 CGA830 Nonfluorescing longpass filter transmits gt 830 nm green orange OF1 CGA475 Nonfluorescing longpass filter transmits gt 475 nm yellow pink 4 Collimating Mirror specify Standard or SAG The collimating mirror is matched to the 0 22 numerical aperture of our standard optical fibers Light reflects from this mirror as a collimated beam toward the grating You can opt to install a standard mirror or a NIR enhancing but UV absorbing SAG mirror SAG mirrors are often specified for flu
86. m of the spectral data This checksum can be used to test the validity of the spectral data and its use is recommended when reliable data scans are required Command Syntax k DATA WORD Value 0 Do not transmit checksum value 0 transmit checksum value at end of scan Response ACK or NAK Default value 0 Version Number Query Returns the version number of the code running on the microcontroller A returned value of 1000 is interpreted as 1 00 0 Command Syntax V Response ACK followed by DATA WORD Default value N A Set FPGA Register Value Sets the appropriate register within the FPGA The list of register setting is in the USB command set information This command requires two data values one to specify the register and the next to specify the value Command Syntax W DATA WORD 1 DATA WORD 2 Data Word 1 FPGA Register address Value j Data Word 2 FPGA Register Value Response ACK or NAK Default value N A 114 225 00000 000 11 201505b Kocen pori ICs 8 Firmware and Advanced Communications ASCII Data Mode Sets the mode in which data values are interpreted to be ASCII Only unsigned integer values 0 65535 are allowed in this mode and the data values are terminated with a carriage return ASCII 13 or linefeed ASCII 10 In this mode the Flame echoes the command and data values back out the
87. mpt you to take your reference and darks Alternatively you can use the quick dark and quick reference features Once a dark and or reference measurement has been set you can update it with the controls on the top bar of the view Quick Reference click to take a reference and set up a new view After clicking it will prompt the user to take a dark This will normalize the signal against the reference in Quick Dark click to take a dark measurement and sets up a new Quick View minus dark view bi Reference click to update the stored reference measurement KE Dark click to update the stored dark measurement Schematic View C B S Y subt 3 Background A D _ gt mira FLMS00005_1 Device FLMS00005 A Ke Subt_5 View_3 225 00000 000 11 201505b Mult_6 Relative A Constant_7 Relativeview_10 29 3 Operation Ocean Sem The schematic view is a graphical interface that allows you to move from device through to processed data There are a few basic components to consider i Device FLMS00005 Devices Each spectrometer will appear as a separate device Right click to open a menu that can generate an acquisition control a TEC if applicable and add other device controls Acquisitions A spectrometer can output one acquisition per detector channel Right click to open menu Mult_6 Nodes These are the building blocks of the
88. n the fourth column calculate the pixel number cubed Independent Values Computed i Dependent from the Regression Variable Variables Output True Wavelength nm Pixel Pixel 4 Pixel 4 3 E Difference 253 65 175 30625 5359375 253 56 0 09 296 73 296 87616 25934336 296 72 0 01 302 15 312 97344 30371328 302 40 0 25 313 16 342 116964 40001688 313 02 0 13 334 15 402 161604 64964808 334 19 0 05 365 02 490 240100 117649000 365 05 0 04 404 66 604 364816 220348864 404 67 0 01 407 78 613 375769 230346397 407 78 0 00 435 84 694 481636 334255384 435 65 0 19 546 07 1022 1044484 1067462648 546 13 0 06 576 96 1116 1245456 1389928896 577 05 0 09 579 07 1122 1258884 1412467848 579 01 0 06 696 54 1491 2223081 3314613771 696 70 0 15 706 72 1523 2319529 3532642667 706 62 0 10 727 29 1590 2528100 4019679000 727 24 0 06 738 40 1627 2647129 4306878883 738 53 0 13 751 47 1669 2785561 4649101309 751 27 0 19 5 Use the spreadsheet or calculator to calculate the wavelength calibration coefficients In the spreadsheet program find the functions to perform linear regressions If using Quattro Pro look under Tools Advanced Math If using Excel look under Analysis ToolPak 6 Select the true wavelength as the dependent variable Y Select the pixel number pixel number squared and the pixel number cubed as the independent variables X After executing the regression you will obtain an output similar to the one shown below Number
89. nce Below is a typical absorbance set up shown with a cuvette and a broadband light source Flow cells can be used interchangeably with the cuvette holder for liquid or gaseous samples Absorbance is typically a relative measurement comparing the spectrum from the sample to a reference Absorbance is commonly used for concentration measurements DH 2000 BAL Computer Flame Spectrometer QP450 0 25 XSR fiber QP450 0 25 XSR fiber Typical Absorbance Set Up 225 00000 000 11 201505b 17 AL cean Sm 2 Installation and Setup Common UV Vis Applications e Quantification of DNA amp proteins in life science samples e Concentration of solutions amp gaseous samples e Identification of trace gases in a mixture Reflectance and Transmission Reflectance spectroscopy compares the relative level of light in reflected off a sample relative to a reference Typically a reflectance standard is used to set the reference level of 100 Transmission is similar but compares the light transmitted through a sample rather than reflected off it Typically Reflectance uses a fiber optic probe attached to a light source and a spectrometer Transmission measurements may use a transmission probe cuvettes or flow cells similar to absorbance measurements Tablet PC Flame Spectrometer Tungsten Halogen Light Source ail e Reflection Probe with Holder A Reflectance Set Up with Probe Reflectance Standar
90. nchronization Trigger Mode Data Value 3 gt External Hardware Edge Trigger Mode Byte Format Byte 0 Byte 1 Byte 2 Ox0A Data Value LSB Data Value MSB Query Number of Plug in Accessories To query the number of preset plug in accessories use the Query Plug in Identifiers command Ox0C below matching plug in IDs to the known IDs Query Plug in Identifiers Queries the Plug in accessories identifiers This command returns 7 bytes with the last byte always being zero at this point Each of the first 6 bytes correspond to Ocean Optics compatible devices which responded appropriately for DC addresses 2 through 7 respectively The DC addresses are reserved for various categories of devices and the value for each category is shown below DC addresses 0 1 are reserved for loading program code from EEPROMS Byte Format Byte 0 0x0C 225 00000 000 11 201505b 75 Ocean Sem 8 Firmware and Advanced Communications Return Format The data is returned in Binary format and read in by the host through End Point 7 Byte 0 Byte 1 Byte 5 Byte 6 Value I C address 2 Value I C address 3 Value I C address 7 0x00 Detect Plug ins Reads all of the plug in accessories that are plugged into the DC bus No data values are returned Byte Format Byte 0 0x0D LED Status Sets the Flame LEDs as follows Data Byte 0 gt LE
91. nchronizing events 58 225 00000 000 11 201505b Ocean Sorem timing signals 57 transmission 18 triggering 58 modes 58 troubleshooting 31 Linux systems 33 Mac systems 33 Windows systems 32 typical set ups 17 USB command summary FLAME T 94 USB Commannd Summary FLAME S 68 225 00000 000 11 201505b Index W warranty vi Wavelength Calibration about 61 Wavelength Calibration Data File 9 Wavelength Calibration Data Sheet 9 wavelength range 40 121 Ocean Crm Index 122 225 00000 000 11 201505b
92. ntil the original spectrum is read by the user 225 00000 000 11 201505b 59 6 Technical Specifications be e Flame Timetable for Hardware Edge Trigger Mode Time between Trigger Rising Edges Minimum Trigger High d 00 ns 1 TRIGGER Integration in Progress Programmable Trigger Delay 1 l 0 32 768 ms ke WEEN A 6 RE A 8 WA Reset CCD 8 9 us Integration e Readout Data e Time 1 Detector to FIFO I l 900 us l ppp UR 1 Additional Delay 0 us Initialize CCD Binning 9 000 us Integration time 1 000 us Read Detector 900 us Minimum Idle States 1 000 us Min Trigger Cycle 2 909 us Min Trigger Cycle 2 909 us Max Trigger Rate 344 Hz I 1 Idle 4 Read FIFO Data 1 Time 8 1ms _ See DD4 Accessory Connector Pinout Diagram to locate the pins to set up triggering 60 225 00000 000 11 201505b Chapter 7 Calibration Overview This chapter provides information for performing your own wavelength calibration and irradiance calibration An EEPROM flash memory chip in each Flame contains wavelength calibration coefficients linearity coefficients and a serial number unique to each individual spectrometer The spectrometer operating software application reads these values directly from the spectrometer enabling the ability to hot swap spectrometers between computers without entering the spectrometer coefficients manually on each computer USB Programmer software is freely a
93. ntinuous Strobe LSB Register 4800 0 OxFFFF 48MHz 0x10 Integration Period Base Clock Divisor 480 0 OxFFFF 48MHz 0x10 Integration Period LSB Register 480 0 OxFFFF 1KHz Set base_clk or base_clkx2 0x14 0 base_clk 0 0 1 N A 1 base_clkx2 Integration Period 0x18 Integration Clock Timer Divisor 600 0 OxFFFF Base Clock see Register 0x10 0x18 Integration Period MSB Register 0x20 Reserved Hardware Trigger Delay Number of Master Clock cycles to delay when in 0x28 External Hardware Trigger mode 0 0 OxFFFF before the start of the integration period Hardware Trigger Delay Delay the 0x28 start of integration from the rising edge 0 0 OxFFFF of the trigger in 500ns increments Trigger Mode 3 0 Free Running 0x2C 1 External Hardware Level Trigger 0 0 4 N A 1 External Synchronization Trigger 3 External Hardware Edge Trigger 0x30 Reserved 225 00000 000 11 201505b 79 Ocean Sem 8 Firmware and Advanced Communications e Default Min Max Time Base Register Description Value Value Value Address 0x38 Single Strobe High Clock Transition 4 0 OxFEFF 2MHz Delay Count Single Strobe Low Clock Transition 0x3C Delay Count 5 0 OxFFFF 2MHz 0x40 Lamp Enable 0 0 1 N A GPIO Mux Register 0x48 0 pin is GPIO pin 0 0 OxO3FF N A 1 pin is alternate function GPIO Output Enable 0x50 1 pin is output 0 0 OxO3FF N A O pin is input GPIO Data Register 0x54 For Output Write value o
94. ny variants of the Flame Spectrometer exist Ocean Optics offers both preconfigured units as well as custom configured units enabling you to order a customized spectrometer optimized for your application You can tell which kind of spectrometer you have by looking at the product code located on the bottom of your spectrometer You can find more information about the various components and possible configuration options in Chapter 5 How the Flame Spectrometer Works Product Code Taxonomy Spectrometer Family Detector Type Preconfigured or Custom UV VIS VIS NIR XR S Sony ILX511B CUSTOM FLAME UV VIS VIS NIR XR T Toshiba TCD1304AP CUSTOM Preconfigured Models FLAME S Preconfigured Models Sony ILX511B Detector Filters amp Detector Resolution std slit Grating nm ui Options FLAME S UV VIS OSF 200 FLAME S UV VIS 850 General ES Purpose FLAME S VIS NIR OSF 350 FLAME S VIS NIR 1000 ES Extended FLAME Sh OSF 200 Range FLAME S XR1 ES ER 6 225 00000 000 11 201505b Ocean Sore 1 Introduction FLAME T Preconfigured Models Toshiba TCD1304TP Detector Filters amp Detector Options Resolution std slit Grating Mirror nm Type FLAME T UV VIS 200 850 FLAME T UV VIS ES General Purpose ELAME T VIS NIR 350 1000 FLAME T VIS NIR ES FLAME T XR1 Extended Range FLAME T
95. o teach students the basic principles of spectroscopy a research lab looking to make a breakthrough a scientist working in the field to study the world around us or an engineer working to integrate a spectrometer into an OEM system the Flame will provide you with the performance and features you need to make your UV Vis spectroscopy application successful 225 00000 000 11 201505b 1 Ocean Sm 1 Introduction Product Features e Incredibly configurable with over 1 billion possible off the shelf configurations across the wavelength range 190 1100nm Balanced throughput resolution and range to optimize the spectrometer for your application e Works seamlessly with Ocean Optics large range of light sources accessories and software e Easy to use Plug and play via the micro USB connection e User interchangeable slit allows you to vary the resolution and throughput of the spectrometer on demand In seconds go from high resolution to high throughput using the same spectrometer e Indicator LEDs show the power and data transfer status of the spectrometer at all times e Compact and lightweight The go anywhere spectrometer for the lab and in the field e The introduction of new high tech manufacturing methods has dramatically improved optical alignment accuracy resulting in improved unit to unit performance and reproducibility e High thermal stability allows for accurate and repeatable measurements in demanding environments
96. oftware to install the drivers What do do now The steps to take to resolve this issue differ depending on your computer s operating system Microsoft Windows Operating Systems If you connected your Ocean Optics Flame device to the computer prior to installing your Ocean Optics software application on a Windows platform you may encounter installation issues that you must correct before your Ocean Optics device will operate properly Follow the applicable steps below to remove the incorrectly installed device device driver and installation files Note If these procedures do not correct your device driver problem you must obtain the Correcting Device Driver Issues document from the Ocean Optics website http oceanoptics com wp content uploads Correcting Device Driver Issues pdf 32 225 00000 000 11 201505b Ocean Ear Remove the Unknown Device from Windows Device Manager gt Procedure 1 Open Windows Device Manager Consult the Windows operating instructions for your computer for directions if needed 2 Locate the Universal Serial Bus Devices option and expand the Universal Serial Bus Devices selection by clicking on the sign to the immediate left Note Improperly installed USB devices can also appear under the Universal Serial Bus Controller option Be sure to check this location if you cannot locate the unknown device 3 Locate the unknown device marked with a large question mark Right
97. olate and correct common problems Chapter 5 How the Flame Describes how the Flame operates illustrating the various Spectrometer Works parts and functions Chapter 6 Technical Contains technical specifications and connector pinouts for the Specifications Flame Spectrometer Chapter 7 Calibration Provides instructions for calibrating the Flame Spectrometer Chapter 8 Firmware and Contains a description of firmware commands Advanced Communications 225 00000 000 11 201505b v Ocean ROS ICS About This Manual Product Related Documentation You can access documentation for Ocean Optics products by visiting our website at http www oceanoptics com Select Support gt Technical Documents then choose the appropriate document from the available drop down lists Document for Document Location OceanView software http oceanoptics com wp content uploads OceanViewlO pdf SpectraSuite software http oceanoptics com wp content uploads SpectraSuite pdf HR 4 Breakout Box http oceanoptics com wp content uploads HR 4 Breakout Box pdf External triggering http oceanoptics com wp content uploads External Triggering Options Firmware3 0andAbove pdf Replacing the slit http oceanoptics com wp content uploads INTSMA Slit pdf Device driver issues http oceanoptics com wp content uploads Correcting Device Driver Issues pdf Ocean Optics offers a Glossary of spectroscopy terms to help you
98. on Product Identification and Authentication Quality Control and Process Monitoring Defect Identification Raw Material Inspection Verification Testing Environmental Monitoring Air and Water Quality Analysis Remote Sensing Volcanic Research You can find more information about applications of UV Vis spectroscopy and the Flame at www oceanoptics com 225 00000 000 11 201505b Ocean Sem 1 Introduction Specifications Summary Specification FLAME S FLAME T SPECTROSCOPIC Optical resolution 0 1 10 0 nm FWHM configuration dependent Signal to noise ratio 250 1 300 1 Integration time 1 ms 65 seconds 3 8 ms 10 seconds Stray light lt 0 05 at 600 nm lt 0 10 at 435 nm Corrected linearity gt 99 8 ELECTRONIC A D resolution 16 bit Power requirement spectrometer functions 250 mA 5 VDC Inputs Outputs 8 x digital user programmable GPIOs Trigger modes 4 modes Strobe functions Yes Gated delay feature Yes Connectors Micro USB and JAE DD4 40 pin connector DETECTOR Detector Sony ILX511B linear silicon Toshiba TCD1304AP linear CCD array CCD array Detector range 190 1100 nm Pixels 2048 pixels 3648 pixels PHYSICAL Dimensions 88 9 mm x 63 5 mm x 31 9 Weight 265 g 225 00000 000 11 201505b Ocean ROS ICS 1 Introduction Product Versions Ma
99. on AWL V A Array coating Mfg W Array wavelength VIS UV OFLV L L2 lens installed V CPLD Version 17 Auto nulling configuration information 18 Startup Baud rate entry 19 30 Reserved Response ACK or NAK Default value N A To query the constants use the x DATA WORD format to specify the desired constant 116 225 00000 000 11 201505b Ocean QORN Koco tip Optics 8 Firmware and Advanced Communications Query Variable Returns the current value of the parameter specified The syntax of this command requires two ASCII characters The second ASCII character corresponds to the command character which sets the parameter of interest acceptable values are B A I K T J y A special case of this command is x lower case and W which requires an additional data word be passed to indicate which calibration constant is to be queried Command Syntax ASCII character Response ACK followed by DATA WORD Default value N A Examples Below are examples on how to use some of the commands Commands are in BOLD and descriptions are in parenthesis For clarity the commands are shown in the ASCII mode a command instead of the default binary mode In ASCII mode the Flame transmits a gt prompt that is useful to determine when it is ready to accept a command The desired operating conditions are acquire spectra from the spectrometer wit
100. on simple fast operation via the micro field including air and water quality USB connection lets users take the monitoring and solar irradiance measurement to the sample Ease of OEMs and developers who need to Engineering labs developers OEM Integration integrate a spectrometer as part of a manufacturers works with LabVIEW system via USB or RS 232 and other design platforms Typical Applications Application Area Examples Light Laser LED Laser Characterization LED Measurement Light Metrology Measurement Research and Education EELER Basic Research Teaching Labs for Physics Chemistry Biomed Life Sciences Biotechnology Medical Diagnostics Protein and Nucleic Acid Analysis Biomaterial Analysis Materials Identification Metallurgical Analysis Polymer Analysis Semiconductor Materials Analysis Semiconductors Processing and Thin Film Plasma Monitoring 225 00000 000 11 201505b 3 1 Introduction Ocean CES Ics Application Area Examples Metrology Process Endpoint Detection Thickness Measurement Farm to Table Technologies Agricultural Measurements and Monitoring Food and Beverage Quality Control Food Safety Energy Technologies Biofuels Analysis Mining and Exploration Oil and Petroleum Analysis Photovoltaic Analysis Solar Simulators Anti Counterfeit Testing and Qualificati
101. operating parameters When executed this command determines the amount of memory required If sufficient memory does not exist an ETX ASCII 3 is immediately returned and no spectra are acquired An STX ASCII 2 is sent once the data is acquired and stored If the Data Storage Mode value is 0 then the data is transmitted immediately If the Scans to Accumulate is 1 then the data is returned as WORDs However if it is greater than 1 then the data is returned as DWORDs to avoid overflow Command Syntax S If successful STX followed by data If unsuccessful ETX The format of returned spectra includes a header to indicate scan number channel number pixel mode etc The format is as follows Response 112 225 00000 000 11 201505b ny kee es WORD OxFFFF start of spectrum WORD Data size flag O Data is WORDS 1 Data is DWORDs WORD Number of Scans Accumulated WORD Integration time in milliseconds WORD FPGA Established Baseline value MSW WORD FPGA Established Baseline value LSW WORD pixel mode WORDs if pixel mode not 0 indicates parameters passed to the Pixel Mode command P D WORDs spectral data depending on Data size flag WORD OxFFFD end of spectrum Trigger Mode 8 Firmware and Advanced Communications Sets the Flame s external trigger mode to the value specified Command Syntax T DATA WORD 0 Normal Continuously scanning 1 Software trigger Value 2 External Syn
102. or if you connected the Flame to the computer before installing the software consult Chapter 4 Troubleshooting 12 Note The Flame driver appears as USB2000 to your computer since a common driver is used to ensure backwards and forwards compatibility This does not affect functionality 225 00000 000 11 201505b Ocean gt Optics 2 Installation and Setup Hardware Features Flame LEDs The Flame features two indicator lights that operate as shown below LED Steady Flashing red Unit is on N A green Unit is ready Unit is acquiring data Note that LEDs can be turned off in OceanView or by using a firmware command Change the Slit The Flame allows you to change your spectrometer s slit to match your application requirements There s no need to calibrate your spectrometer when changing the slit just install and start measuring There is one exception to this You cannot change from a standard slit to a slit with a filter because it changes the optical focus and wavelength calibration of the spectrometer In this case you would need to send the spectrometer back to Ocean Optics A filter must be ordered for each slit if your application requires a filter and the spectrometer needs to be calibrated and focused with the filter installed This only applies to filters installed inside the slit assembly gt Procedure 1 Find the SMA connector If a fiber is attached remove it CES Miniatu
103. orescence These mirrors absorb nearly all UV light which reduces the effects of excitation scattering in fluorescence measurements Unlike typical silver coated mirrors the SAG mirrors won t oxidize They have excellent reflectivity more than 95 across the VIS NIR Specify standard or SAG mirrors when ordering your spectrometer 225 00000 000 11 201505b 39 Ocean Sem 5 How the Flame Spectrometer Works 100 5 EA TTT 80 60 40 Reflectance 20 i sl T Lem SA T T gt a T 3 200 nm 500 nm ym 2 um 5 um Wavelength Reflectance vs Wavelength for Aluminum Gold and Silver Mirrors By Bob Mellish in Wikipedia 5 Grating and Wavelength Range specify grating and starting wavelength We install the grating on a platform that we then rotate to select the starting wavelength you have specified Then we permanently fix the grating in place to eliminate mechanical shifts or drift Use our online Range and Resolution Calculator to find out how your grating choice affects spectral range and optical resolution by viewing the grating efficiency curves The available gratings are summarized below Grating Intended Use Groove Density Spectral Range Blaze Wavelength Best Efficiency Number gt 30 opo e m om w e oms e m o ea Co see se JL mes 0am Lens e oa a oan ere e a az rem mara a a amzor nem anamen 40 225 00000 000 11 201505b Kocen ip Optics 5 How the Flame
104. p and whenever the Plug in Detect command is issued Byte Format Byte 0 0x0B Return Format The data is returned in Binary format and read in by the host through End Point 7 225 00000 000 11 201505b 99 8 Firmware and Advanced Communications Byte 0 Value BYTE Query Plug in Identifiers Queries the Plug in accessories identifiers This command returns 7 bytes with the last byte always being zero at this point Each of the first 6 bytes correspond to Ocean Optics compatible devices which responded appropriately for DC addresses 2 through 7 respectively The DC address are reserved for various categories of devices and the value for each category is shown below 1 Caddresses 0 1 are reserved for loading program code from EEPROMS Byte Format Byte 0 0x0C Return Format Ocean Sem The data is returned in Binary format and read in by the host through End Point 7 Byte 0 Byte 1 Byte 5 Byte 6 Value QC Value EC Value C 0x00 address 2 address 3 address 7 Detect Plug ins Reads all of the plug in accessories that are plugged into the DC bus No data values are returned Byte Format Byte 0 0x0D LED Status Sets the Flame LEDs as follows Data Byte 0 LEDs Off Data Byte 1 gt LEDs On Byte Format Byte 0 Byte 1 0x12 Data byte 100 225 00000 000 11 201505b Ocean tip O
105. ptics 8 Firmware and Advanced Communications General DC Read Performs a general purpose read on the I2C pins for interfacing to attached peripherals The time to complete the command is determined by the amount of data transferred and the response time of the peripheral The I2C bus runs at 400KHz The maximum number of bytes that can be read is 61 Command Byte Format Byte0 Byte 1 Byte 2 0x60 IC Address Bytes to Read Return Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Byte N 3 CC Results IC Address Bytes to Read Data ByteO Data byte N 2C Result Value Description 0 CC bus Idle IC bus Sending Data IC bus Receiving Data C bus Receiving first byte of string CC bus in waiting for STOP condition C experienced Bit Error CC experience a Not Acknowledge NAK Condition CC experienced successful transfer CC bus timed out O oO N I OD oO AIN General 12C Write Performs a general purpose write on the DC pins for interfacing to attached peripherals The time to complete the command is determined by the amount of data transferred and the response time of the peripheral The DC bus runs at 400KHz The results codes are described above Command Byte Format Byte 0 Byte 1 Byte 2 Byte 3 su Byte N 3 0 IC Address Bytes to Write Data vibe Data byte N x61 Byte 0 225 00000 000 11 2
106. rating 1 or 2 DET2B 200 1100 Sony ILX511 detector installed with 200 850 nm variable FLAME S longpass filter and UV2 quartz window best for UV VIS systems configured with XR 1 grating DET2B 350 1000 Sony ILX511 detector installed with 350 1000 nm variable FLAME S longpass filter best for VIS systems configured with Grating 2 or 3 DET2B UV Sony ILX511 detector installed with UV2 FLAME S quartz window best for systems configured for lt 360nm DET2B VIS Sony ILX511 detector installed with VIS BK7 window best FLAME S for systems configured for gt 400nm DET4 200 535 Toshiba TCD1304AP detector installed with Custom OFLV FLAME T Coated Window Assembly for Grating 5 and Grating 5U S bench DET4 200 850 Toshiba TCD1304AP detector installed with 200 850 nm FLAME T variable longpass filter and UV2 quartz window best for systems configured with Grating 1 or 2 DET4 200 1100 Toshiba TCD1304AP detector installed with 200 850 nm FLAME T variable longpass filter and UV4 quartz window best for systems configured with XR 1 grating DET4 350 1000 Toshiba TCD1304AP detector installed with 350 1000 nm FLAME T variable longpass filter best for VIS systems configured with Grating 2 or 3 DET4 UV Toshiba TCD1304AP detector installed with UV4 quartz FLAME T window best for systems configured for lt 360 nm DET4 VIS Toshiba TCD1304AP detector installed with VIS BK7 quartz FLAME T window best for systems configure
107. re Fiber Optic which can communicate via the Universal Serial Bus or RS 232 This document contains the necessary command information for controlling the Flame via the RS 232 interface Hardware Description The Flame utilizes a Cypress FX2 microcontroller which has a high speed 8051 combined with an USB ASIC Program code and data coefficients are stored in external E7PROM which are loaded at boot up via the I C bus Spectral Memory Storage The Flame can store a single spectrum in the spectral data section While spectra are being accumulated it is being co added to the existing spectra in memory Instruction Set Command Syntax The list of the commands is shown in the following table along with the microcode version number they were introduced with All commands consist of an ASCII character passed over the serial port followed by some data The length of the data depends on the command The format for the data is either ASCII or binary default The ASCII mode is set with the a command and the binary mode with the b command To insure accurate communications all 225 00000 000 11 201505b 107 Kocen 8 Firmware and Advanced Communications WP Opt ics commands respond with an ACK ASCII 6 for an acceptable command or a NAK ASCII 21 for an unacceptable command i e data value specified out of range In the ASCII data value mode the Flame echoes the command back out the RS 232 port In binary mode all da
108. re Spectrometer 225 00000 000 11 201505b 13 Ocean ZeInstalation and Sep AECH 2 Use the Allen key to remove the 2 the screws attaching the slit to the spectrometer 3 Pull the slit out of the spectrometer 4 Put the new INTSMA slit connector into the spectrometer with the key of the connector on the left side 5 Install the 2 screws again Use the Allen key to tighten the screws carefully do not over tighten 6 If necessary connect the fiber again Accessories Ocean Optics provides a range of standard cables and accessories that connect the Flame to our large range of sampling and light source accessories Items specifically designed for the Flame are described here they are not provided with the Flame spectrometer and must be purchased separately Visit us at www oceanoptics com for a complete list of products available for all of your spectroscopy needs Cables and Connectors Cables are available to connect your Flame Spectrometer to accessories e Accessory cable for light sources and other accessories DB15 Connector Cable e Accessory cable for HR type connector PAK50 Connector Cable e Breakout board DD4 BREAKOUT BOARD This breaks out the 40 pin DD4 connector to a set of header pins that can be used to wire each pin as required 14 225 00000 000 11 201505b Ocean Som 2 Installation and Setup DB15 Connector Cable FLAME CBL DD4P DB15P This cable connects the Flame to existing Ocean Opt
109. ring for 62 procedure 62 calibration coefficients saving in USB mode 64 CCD 54 detector 54 detector reset 57 well depth 55 compliance vii 225 00000 000 11 201505b Index detector 41 specifications 42 detector window 42 document audience v purpose v summary v document version vi E electrical pinout 49 external triggering 58 F features 2 fiber optic connector 38 filter 42 firmware 67 FLAME S 67 FLAME T 93 fluorescence 19 grating 40 H hardware features 13 setup 11 119 Index I2C 52 installation 9 software 10 internal operation 56 irradiance 20 ISO certification vii L LEDs 13 lens 41 longpass filter 39 measurement dark 29 reference 29 measurement techniques 17 mechanical diagram 48 memory chip 61 methods 28 mirror collimating 39 focusing 41 O OceanView 11 connect Flame 25 launch 23 main screen 24 product key 32 120 Ocean Sorm packing list 9 patent notice vi pixel definition 56 product features 2 introduction 1 versions 6 product related documentation vi projects 28 Quick View mode 26 Reflectance 18 repairs 34 S schematic view 29 serial port FLAME S 83 FLAME T 107 servicing 35 setup 9 signal averaging 55 slit 16 38 change 13 specifications 45 summary 5 spectrometer how it works 37 spectrometer configuration 34 SPI 52 strobe 57 continuous 58 single 57 sy
110. rom the detector and is proportional to the voltage induced by the light falling on the detector It is very important to realize that this is uncalibrated data and that a counts signal does not represent a particular power or energy from one wavelength to the next Because the response of the detector is linear twice the counts at a particular wavelength do indicate that the amount of light at that wavelength has doubled relative to another wavelength However a small peak relative to a big peak does not indicate that there is less or more light at a particular wavelength relative to another in absolute terms To understand the true relationship you need to do a relative measurement including relative irradiance or if you want a quantified result an absolute irradiance measurement Continuous and Single Acquisitions There are two sets of controls for taking or pausing acquisitions The set on the Acquisition group window allows you to control each spectrometer individually The set on the top bar is a global control that will allow you to start and pause all spectrometers currently attached gt Acquire data continuously Pil Take a single acquisition and then pause fl Pause all acquisitions 26 225 00000 000 11 201505b kee i Save Data 3 Operation D Configure Saving set saving parameters and file type file directory and file naming convention Once selected the file directory will persist until
111. s of importance are noted Regression Statistics Multiple R 0 999999831 R Square 0 999999663 _ R Squared Adjusted R Square 0 999999607 Standard Error 0 125540214 Observations 22 Intercept Coefficients SE Error Intercept 190 473993 0 36904753 First coefficient X Variable 1 0 36263983 001684745 225 00000 000 11 201505b 63 7 Calibration 7 Ocean Sm X Variable 2 1 174416E 0 8 35279E 07 X Variable 2 656608E 1 Second coefficient Third coefficient Record the Intercept as well as the First Second and Third Coefficients Additionally look at the value for R squared It should be very close to 1 If not you have most likely assigned one of your wavelengths incorrectly Keep these values at hand Saving the New Calibration Coefficients USB Mode Ocean Optics programs wavelength calibration coefficients unique to each Flame onto an EEPROM memory chip in the Flame You can overwrite old calibration coefficients on the EEPROM if you are using the Flame via the USB port 64 gt Procedure To save wavelength calibration coefficients using the USB mode perform the following St Gs Se N steps Ensure that the Flame is connected to the computer and that you have closed all other applications Point your browser to http www oceanoptics com technical softwaredownloads asp and scroll down to Microcode Select USB EEPROM Programmer Save the setup file to your computer Run the
112. s parts and labor needed to repair manufacturing defects that occur during the warranty period We also will cover the costs of shipping warranty related repairs from our customers to Ocean Optics and from us to our customers ISO Certification Ocean Optics the industry leader in miniature photonics has been certified for ISO 9001 2008 certification applicable to the design and manufacture of electro optical equipment since 2009 About This Manual Compliance A WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures FCC COMPLIANCE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his own expense a WARNING The authority to operate this equipment is conditioned by the requirement that no modifications will be made to the equipment unle
113. s they were when the software was last closed 3 Operation e Spectroscopy Application Wizards Use this function to set up a measurement using simple step by step wizards A large range of applications is available OceanView Main Screen No matter what route you take on start up you will soon end up on the OceanView main screen This is where you can set and view acquisitions save and load data and save projects File Window He ASUS Gp pi il egen Group vorm Gives Ges F12QQQ8 enn opIne Ge I 1 Acquisition Group Window Use to set acquisition parameters such as integration time Controls the spectrometer acquisition 2 Schematic View Schematic view graphically displays the flow of information from the spectrometer to the view Use nodes to mathematically modify the data to create processed measurements methods This function is extremely flexible and incredibly powerful 3 View Display Display your data view save and display controls as well as other features such as peak finder and quick dark amp reference 4 Global Controls Control all spectrometers synchronously save projects and start a new application wizard 5 Saved Data Displays data saved in the active save file path Preview data store notes and load overlays directly to the active view Click to open 24 225 00000 000 11 201505b Ocean UN Connect the Flame in OceanView The Flame should automatical
114. s transferred in MSB LSB order This command requires 100us to complete the calling program needs to delay for this length of time before issuing another command In some instances other commands will also write to these registers i e integration time in these cases the user has the options of setting the parameters through 2 different methods Byte Format Byte 0 Byte 1 Byte 2 Byte 3 Ox6A Register Value Data Byte LSB Data Byte MSB 102 225 00000 000 11 201505b Ocean ip Optics 8 Firmware and Advanced Communications i Time Base Register Bi Sea Max Value Description alue alue Address 0x00 Mase Clack Conner L 1 OxFFFF 48MHz Divisor FPGA Firmware 0x04 Version Read Only Continuous tani Get Strobe Base ontinuous Stro Clock 0x08 Timer Interval Divisor zii S SSES see Register 0x0C Continuous Strobe 0x0C Base Clock Divisor 4800 0 OxFFFF 48MHz Integration Period 0x10 Base Clock Divisor 480 0 OxFFFF 48MHz Set base_clk or base_clkx2 7 i N A oxis 0 base_clk 0 1 base_clkx2 Integration Sech Period Base 0x18 ntegration oc 600 0 OxFFFF Clock Timer Divisor i see Register 0x10 0x20 Reserved Hardware Trigger Delay Number of Master Clock cycles to delay when in 0x28 External Hardware 0 0 OxFFFF 2MHz Trigger mode before the start of the integration period Trigger Mode 0 Free Running ec Leona 0 0 2 N A Synchronization 2 External Hardwar
115. ss the changes or modifications are expressly approved by the manufacturer 225 00000 000 11 201505b vii Ocean Spee Manual AECH viii 225 00000 000 11 201505b Chapter 1 Introduction Product Introduction The Flame Spectrometer is the latest generation of Ocean Optics ubiquitous Czerny Turner design With the release of the world s first miniature spectrometer in 1993 Ocean Optics helped to make spectroscopy portable inexpensive and flexible in a way that was never before possible Researchers educators and OEM manufacturers embraced the technology and have used it in ways we never thought possible Aflame ROSER Miniature Spectrometer Flame Spectrometer Now fueled by our passion for solving problems and inspired by the feedback of our customers we have reinvented our core miniature spectrometer platform to meet the most demanding challenges of today s applications Flame combines our heritage creativity and insight in a way that delivers the power of miniature modular spectroscopy without compromise Flame is built using industry leading manufacturing techniques that help deliver high thermal stability and low unit to unit variation without compromising the flexibility and configurability that are the hallmark of the design New features such as interchangeable slits indicator LEDs and simpler device connectors deliver more freedom and less frustration Whether you are an educator looking for an instrument t
116. t Packet 0 Byte 0 Byte 1 Byte 2 Byte 3 Pixel 0 LSB Pixel 0 MSB Pixel 1 LSB Pixel 2 MSB Byte 510 Byte 511 Pixel 255 LSB Pixel 255 MSB Packet 15 Synchronization Packet 1 byte Byte 0 0x69 USB Full Speed 12Mbps Packet Format In this mode all data is read from EP2In The pixel and packet format is shown below Packet End Point Bytes Pixels 0 EP2In 64 0 31 1 EP2in 64 32 63 2 EP2In 64 64 95 EP2in 64 119 EP2in 64 3808 3839 120 EP2In 1 Sync Packet 98 225 00000 000 11 201505b Ocean Optics 8 Firmware and Advanced Communications Packet 0 Byte 0 Byte 1 Byte 2 Byte 3 Pixel 0 LSB Pixel 0 MSB Pixel 1 LSB Pixel 2 MSB Byte 62 Byte 63 Pixel 31 LSB Pixel 31 MSB Packet 120 Synchronization Packet 1 byte Byte 0 0x69 Set Trigger Mode Sets the Flame Trigger mode to one of the following states If an unacceptable value is passed then the trigger state is unchanged Data Value 0 3 Normal Free running Mode Data Value 1 gt Software Trigger Mode Data Value 2 3 External Synchronization Trigger Mode Data Value 3 gt External Hardware Trigger Mode Byte Format Byte 0 Byte 1 Byte 2 Ox0A Data Value LSB Data Value MSB Query Number of Plug in Accessories Queries the number of Plug in accessories preset This is determined at power u
117. ta except where noted passes as 16 bit unsigned integers WORDs with the MSB followed by the LSB By issuing the v command Version number query the data mode can be determined by viewing the response ASCII or binary In a typical data acquisition session the user sends commands to implement the desired spectral acquisition parameters integration time etc Then the user sends commands to acquire spectra S command with the previously set parameters If necessary the baud rate can be changed at the beginning of this sequence to speed up the data transmission process Command Summary e Set Pixel Boxcar 1 00 0 Set FPGA Register Information 1 00 0 108 225 00000 000 11 201505b Ocean tip Optics 8 Firmware and Advanced Communications X SES E DEER e setea mere teroare um Setintaratonvaie GE vaue anus meremens 100 Command Descriptions A detailed description of all Flame commands follows The 4 indicates a data value which is interpreted as either ASCII or binary default The default value indicates the value of the parameter upon power up Add Scans Sets the number of discrete spectra to be summed together Since the Flame has the ability to return 32 bit values overflow of the raw 16 bit ADC value is not a concern Command Syntax A DATA WORD Response ACK or NAK Range 1 5000 Default value 1 Pixel Boxcar Width Sets the number of pixels to be averaged
118. the integration time while the data is read out through serial shift registers At the end of an integration period the process is repeated 54 225 00000 000 11 201505b Koco WP Opi ICs 6 Technical Specifications When a well is fully depleted by leakage through the back biased photodetector the detector is considered saturated and provides the maximum output level The CCD is a depletion device and thus the output signal is inversely proportional to the input photons The electronics in the Flame invert and amplify this electrical signal CCD Well Depth We strive for a large signal to noise S N in optical measurements so that small signal variations can be observed and a large dynamic range is available The S N in photon noise limited systems is defined and measured as the square root of the number of photons it takes to fill a well to saturation In the Flame the well depth of the CCD pixels is about 160 000 photons providing a S N of 400 1 S N can also be measured as the saturation voltage divided by near saturation RMS noise There is also a fixed readout noise component to all samples The result is a system with a S N of 275 1 There are two ways to achieve a large S N e g 6000 1 in CCD detectors where photon noise is predominant 1 Use a large well device that integrates to saturation over a long period of time until the photon noise is averaged out by the root of 7 multiples of a defined short At 2 Use a small w
119. the version number of the code running on the microcontroller A returned value of 1000 is interpreted as 1 00 0 Command Syntax v Response ACK followed by DATA WORD Default value N A Calibration Constants Writes one of the 32 possible calibration constant to EEPROM The calibration constant is specified by the first DATA WORD which follows the x The calibration constant is stored as an ASCII string with a max length of 15 characters The string is not check to see if it makes sense Command Syntax x DATA WORD ASCII STRING Value DATA WORD Index description 0 Serial Number 1 0 order Wavelength Calibration Coefficient 2 1 order Wavelength Calibration Coefficient 3 2 order Wavelength Calibration Coefficient 4 3 order Wavelength Calibration Coefficient 5 Stray light constant 6 0 order non linearity correction coefficient 7 1 order non linearity correction coefficient 8 2 order non linearity correction coefficient 9 3 order non linearity correction coefficient 10 4 order non linearity correction coefficient 11 5 order non linearity correction coefficient 12 6 order non linearity correction coefficient 13 7 order non linearity correction coefficient 14 Polynomial order of non linearity calibration 15 Optical bench configuration gg fff sss gg Grating 4 fff filter wavelength sss slit size 16 Flame configurati
120. tions You ll find more useful information including a glossary of spectroscopy and spectrometer terms on our website at www oceanoptics com Flame Open Bench 225 00000 000 11 201505b 37 5 How the Flame Spectrometer Works 38 Ocean Sem 1 Fiber Optic Connector Light from a fiber enters the optical bench through the SMA 905 Connector The SMA 905 bulkhead provides a precise location for the end of the optical fiber slit absorbing filter and fiber clad mode aperture While we supply SMA connectors as standard FC connectors are also available See 2 for available options Interchangeable Slit Light passes through the installed slit which acts um The slit is fixed in the SMA 905 bulkhead to sit against the end of a fiber Smaller slit sizes achieve the best optical resolution while larger slits have higher light throughput Slit size is labeled as shown as the entrance aperture Slits come in various widths from 5 um to 200 i 25 Slit Description Pixel Resolution INTSMA 5 5 um wide x 1 mm high 3 0 pixels INTSMA 10 10 um wide x 1 mm high 3 2 pixels INTSMA 25 25 um wide x 1 mm high 4 2 pixels INTSMA 50 50 um wide x 1 mm high 6 5 pixels INTSMA 100 100 um wide x 1 mm high 12 pixels INTSMA 200 200 um wide x 1 mm high 24 pixels INTSMA 000 Interchangeable bulkhead with no slit NA INTSMA KIT Interchangeable SMA Kit connectors 5um 10um 25um NA 50um 100um and 200um
121. together A value of n specifies the averaging of n pixels to the right and n pixels to the left This routine uses 32 bit integers so that intermediate overflow will not occur however the result is truncated to a 16 bit integer prior to transmission of the data This math is performed just prior to each pixel value being transmitted out Values greater than 3 will exceed the idle time between values and slow down the overall transfer process Command Syntax B DATA WORD Response ACK or NAK Range 0 15 Default value 0 225 00000 000 11 201505b 109 Ocean 8 Firmware and Advanced Communications oe Opi is Set Data Compression Specifies whether the data transmitted from the Flame should be compressed to speed data transfer rates For more information on Flame Data Compression see Technical Note 1 Command Syntax G DATA WORD Response ACK or NAK be 0 Compression off SS 10 Compression on Default value 0 Integration Time 16 Bit Sets the Flame s integration time in milliseconds to the value specified This command accepts just a 16 bit value and is expressed in ms for backward compatibility with the USB2000 Use the i command for full 32 bit functionality Command Syntax I 16 bit DATA WORD Response ACK or NAK Range 1 65 000 000 Default value 6ms Integration Time 32 B
122. ts Signal Averaging coe caci iai z cat ol aa a ti iad e aa a aiba i la ba i a 55 uer Le TEE 56 NG lr Le 56 CCD Detector Reset Operation 57 Timing SANS socanta oi ir fa e ci a e m li am 57 Strobe Signals siana sais aaa a a LA ei a i a a i ae eee 57 ele Ee EE 57 Continous Stobe sssini aa dade eat ali a oc dl e a a le a d a 57 Synchronizing Strobe Events 58 External Triggering scosese eu rors ene diame cai deal e ai 58 Triggenng Modesto e aa m do ot i a a a ap i a tii Pa b 58 NOTA see ceaun EE n a a a d dt a a ba d d e DE E a a d d a 58 External Synchronous Trigger Mode mmm nn nn aaa amana 59 External Hardware Level Trigger Mode mn eee eee nnea ee nnaa ee anaae 59 External Hardware Edge Trigger Mode men eee nnaa ee anaa ee anaae 59 Chapter 7 Calibra i Ori sa ear minim ai bi painii 61 KEE eege 61 Wavelength Calibratian ne ae e eea eacat e ca 61 About Wavelength Calibration nenea nea amana ea aaa ana 61 Calibrating the Spectrometer Wavelength men nea eee aaa ana na 62 Preparing for Calibration nene nenea neam ana ana anna ana nana aaa 62 Calibrating the Wavelength of the Spectrometer mme nenea ana nana 62 lradianee Calibra re sect melci ese a ti i n ea al ai a 65 Chapter 8 Firmware and Advanced Communications 67 FLAME S Pinar iii crea ea a ea 3 e le 67 Hardware Description sasa eee ca aaa ne a a p a e a B i a i ing
123. vailable from Ocean Optics http oceanoptics com support software downloads This software can be used to write calibration coefficients to the spectrometer and reload firmware if the spectrometer becomes corrupted Wavelength Calibration This section describes how to calibrate the wavelength of your spectrometer Though each spectrometer is calibrated before it leaves Ocean Optics the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions Ocean Optics recommends periodically recalibrating the Flame About Wavelength Calibration You are going to be solving the following equation which shows that the relationship between pixel number and wavelength is a third order polynomial Ap I GDL GG Lt GO Where A the wavelength of pixel p 1 the wavelength of pixel 0 G the first coefficient nm pixel G the second coefficient nm pixel 225 00000 000 11 201505b 61 Ocean Sem 7 Calibration C the third coefficient nm pixel You will be calculating the value for Jand the three CS Calibrating the Spectrometer Wavelength Preparing for Calibration To recalibrate the wavelength of your spectrometer you need the following components e A light source capable of producing spectral lines Note Ocean Optics HG 1 Mercury Argon lamp is ideal for recalibration If you do not have an HG 1 you need a light source that produces several at least 4 6 spectral lines
124. value 0 88 225 00000 000 11 201505b Ocean Ear Pixel Mode Specifies which pixels are transmitted While all pixels are acquired on every scan this 8 Firmware and Advanced Communications parameter determines which pixels will be transmitted out the serial port Command Syntax P DATA WORD Description Example 0 all 2048 pixels P 0 spaces for clarity 1 every n pixel with no averaging emy 2 N A P 1 lt Enter gt 3 pixel x through y every n pixels Neeme 4 up to 10 randomly selected pixels Pa NA between 0 and 2047 denoted p1 p2 osema p10 x lt Enter gt Nelle y lt Enter gt n lt Enter gt P 4 lt Enter gt n lt Enter gt pi lt Enter gt p2 lt Enter gt p3 lt Enter gt p10 lt Enter gt Response ACK or NAK Default value 0 Since most applications only require a subset of the spectrum this mode can greatly reduce the amount of time required to transmit a spectrum while still providing all of the desired data This mode is helpful when interfacing to PLCs or other processing equipment Spectral Acquisition Acquires spectra with the current set of operating parameters When executed this command determines the amount of memory required If sufficient memory does not exist an ETX ASCII 3 is immediately returned and no spectra are acquired An STX ASCII 2 is sent once the data is acquired and stored If the Data Storage Mode value is 0 then the data
125. yped on the board itself See Product Related Documentation to access the manual for the Breakout Box Interchangeable Slits The Flame offers the capability of changing the slit size to match your measurement needs You can order additional replacement slits either individually or as a kit in various widths from 5 um to 200 um See Change the Slit and Chapter 5 How the Flame Spectrometer Works for more information 16 225 00000 000 11 201505b Kocen ip Optics 2 Installation and Setup Light Sources Cuvette Holders and Other Accessories Ocean Optics supplies a large range of accessories for use with our spectrometers Visit us at www oceanoptics com for a complete list of products available for all of your spectroscopy needs a Fibers Light Sources Integrated Sampling Systems Cuvettes including microfluidic cuvettes Filter Holders amp Filters including Low Pass Band Pass and High Pass DOOD Measurement Techniques Typical Set ups The Flame in conjunction with Ocean Optics light sources and sampling accessories can be used for many different measurement techniques One of the key advantages of modular fiber optic spectroscopy is that you can change components of the system without having to buy a whole new system Here we show a range of typical UV Vis set ups for basic spectroscopy techniques You ll find lots more information about measurement techniques at www oceanoptics com measurementtechnique Absorba

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