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Denville Atlantis Microplate Washer

1. The instrument may only be operated by qualified personnel If a warranty is brought into operation Denville Scientific shall repair or replace any defects which have resulted from faulty material or during production as it check fit No costs shall arise for the client except cargo rates All components found in the original equipment or an adequate and full compatible alternative shall be available for a period of 5 five years after production This warranty refers to the obligations of Denville Scientific and can only be amended upon the written consent of Denville Scientific In original condition the instrument meets all safety regulations for a risk less operation Denville Scientific Instruments can not warrant damages or any resulting costs caused by alterations repairs or modifications of the equipment Issue 01 Page 19 7 7 1 Software Principle Software Navigation Drawing 2 Software Navigation ge lt to scroll through the menus yse service Prime Rinse lt lt gt gt manual DEDE lt j Run Procedure lt lt gt gt Procl enter washi iquid OK No Yes Waste empty No Yes to scrol through the menu EL gt i x Quar The software was designed to make navigation through the different menus as easy as possible The main menus are organized like a wheel which can be turned in both directions by means of the two first ke
2. Yes j No Plate inserted if disabled Yes FK Perform N re Stop S Procedure J Table 4 Run Procedure For details please refer to chapter 9 1 Run Procedure Issue 01 Page 23 7 3 2 Prime Rinse gt gt gt D Prime Rinse Esc i Esc Manual lt Liquid e d Manual EM X prime rinse _ A A Esc Esc Automatic Automat Liquid lt Volume ON Bme Esc Esc Esc Stop Period Liquid lt j Volume Ley Rinse V periodic Rinse a interval e J d Table 5 Prime Rinse For details please refer to chapter 9 2 Issue 01 Page 24 7 33 Define Procedure Table 6 Define Procedure lt lt Def Procedure ae gt y Esc Name lt v o a Esc Liquid zi bee Esc Cycle 1 8 Cycles e el v p ed Esc Final asp time lt v Final as ae ed Esc Dunt if Final asp time t lt 4 Processin pes Esc mode T lt y A Esc Plate lt v p lt lt Esc Ask strip nr lt y Les if P
3. 4 3 Cleaning This instrument is a precision instrument and it requires regular cleaning to ensure the continued precision gt Liquid spillage If any liquid is spilled on the instrument it should be IMMEDIATELY removed so that the liquid does not attack the surface of the housing gt Regular cleaning The housing of the instrument should be cleaned regularly with a mild household cleaning agent Warning Do not use aggressive solutions The area surrounding the plate carrier should be cleaned with a dry cloth gt Fittings and tubing The wash buffer tends to crystallize easily on air contact and can block tubing and fittings Use the automatic rinse program daily and do not leave the tubing filled with wash buffer for an extended period of time 4 4 General features The Atlantis incorporates state of the art technology and software both necessary to allow optimum performance and easy use The Atlantis is designed around your application gt Easy operation Through the menu structure of the on board software The display 2x16 characters and the keyboard with 5 keys provides clear access to all features of the flexible software If desired the washer may also be operated from a PC using the optional Windows based software Issue 01 Page 9 8 or 12 way manifold As standard configuration Additional manifolds available as options The manifold is easily exchanged Depending on the type of strip plate 8 or 12 way s
4. The instrument should be disinfected using a suitable disinfection solution 1 Disconnect the instrument from the mains power supply 2 Carefully wipe all the outside surfaces of the instrument and the plate area with a wad of cotton wool that has been soaked in the disinfection solution Note Ensure that disposable gloves are worn Issue 01 Page 65 3 Place the instrument in to a large plastic bag 4 Place a wad of cotton wool that has been soaked in the disinfection solution in to the plastic bag Note Ensure that the wad is not touching the instrument 5 Close and seal the plastic bag 6 Leave the instrument to stand in the plastic bag for at least 24 hours 7 After the standing time remove the instrument from the plastic bag and wipe all the outside surfaces of the instrument and the plate support area with a wad of cotton wool which has been soaked in a 50 Alcohol solution 8 Repeat the disinfection procedure on any accessories which are also being moved or returned The pumps can be disinfected by dispensing and aspirating a sufficient amount of disinfectant fluid After the disinfection procedure dispense gt 200 ml of distilled water to remove all disinfectant fluid from the liquid carrying parts Issue 01 Page 66 12 Intended Use This device is intended to be used to remove non bound sample sera or to remove reagents from 96 well respectively 384 well microplates or format identical strips By means
5. lt gt Or lt gt Use these keys to toggle between 8 strip 12 strip and 16 strip plate lt enter gt Confirm the selection of plate format and go to the next screen Well shape lt lt ESC Flat Screen 59 Well Shape Selection gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to toggle between flat and round bottom plates lt enter gt Confirm the selection of well shape and go to the next screen Center pos Esc Mov 0 1 mm Screen 60 Center Position Entry This menu appears only if a round bottom plate is selected For round bottom plates it is recommended to position the aspiration needle in the center of the well The center position may be defined by teach in move the plate to the correct position with the lt gt and lt gt keys Issue 01 Page 49 gt Center pos Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes Select Mov to position the plate to the current center position and switch to the teach in mode lt gt Or lt gt Use these keys to enter the center position The plate can be moved in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the center position and go to the next screen Al Mov 0 1 mm Screen 61 Center Position Teach i
6. Def Cycle lt lt gt gt lt new gt Screen 42 Define Cycle Menu This menu allows to define all parameters of a cycle A cycle is required to define a complete wash procedure Issue 01 Page 42 gt Function keys lt F1 gt or lt F2 gt Select another menu lt gt Or lt gt Select lt new gt to define a new cycle or select an existing cycle to edit it lt enter gt Confirm the selection of the cycle and go to the next screen Name Esc Chg 1 Screen 43 Cycle Name Entry The name of the cycle may be changed gt Function keys lt F1 gt or lt F2 gt Select Chg to change the name for the cycle Name Esc Sel Screen 44 Cycle Name Entry gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or Sel to select the current letter number lt gt Or lt gt Use these keys to scroll through the available letters A Z and numbers 0 9 lt enter gt Confirm the name of the cycle and go to the next screen Aspiration time lt lt Esc 5 sec Screen 45 Aspiration Time Entry gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to change the aspiration time the time the Issue 01 Page 43 manifold remains inside a well during aspiration in steps of Isec rang
7. Or lt gt Select lt new gt to define a new procedure or select an existing procedure to edit it lt enter gt Confirm the selection of the procedure and go to the next screen Name Esc Chg 1 Screen 31 Procedure Name Entry The name of the procedure may be changed Issue 01 Page 38 gt Function keys lt F1 gt or lt F2 gt Select Chg to change the name for the procedure Screen 32 Procedure Name Entry gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or Sel to select the current letter number lt gt Or lt gt Use these keys to scroll through the available letters A Z and numbers 0 9 lt enter gt Confirm the name of the procedure and go to the next screen Liquid lt lt Esc Wash 1 Screen 33 Wash Liquid Selection Select the wash liquid to be used for this procedure gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to enter the wash liquid lt enter gt Confirm the wash liquid and go to the next screen Screen 34 Cyclel Selection Select the first cycle to be used for this procedure The cycles must be defined in the Define Cycle Menu prior to the definition of the procedure gt Function keys lt F1 gt or lt F2 gt Select Esc to retu
8. Side pos rear wise asp No lt v Aspiration Ht a Esc lt v a Esc Disp pos lt EYES at a Esc Bottom disp ht lt v a Esc Ovflow disp ht lt li Storing ES Table 8 Define Plate Issue 01 For details please refer to chapter 9 5 Define Plate Menu Page 27 7 3 6 Setup Setup gt gt gt lt A Esc Language lt v Lee Esc Manifold v M Esc Wash liquids ime Te T Er v la Esc Wastfulldet 4 zi v Rinse empty lt lt ld Esc _ e det E v Wash 1 empty if Wash JE det M diuids 1 T zi v Wash2 empty if Wash poss det M liquids 2 P pes v Wash3 empty ed Esc sl det m T E v Esc Liquid warning gt T ee y eee Esc Waste warnin 9 T ee y Eso Esc Plat i d Esc ate Warning T m v ad Esc Keyclick els ME Ww C Storing 3 Table 9 Setup For details please refer to chapter 9 6 Setup Menu Issue 01 Page 28 7 3 7 Service lt lt Service gt gt P lt A Sensors I Esc v Valves Esc po v Pumps I Esc F2 v Platlnit I Esc Y PlatPos Esc p Y MFInit I Esc A
9. v MFPos Esc A v MFAdjust Esc A v PlAdjust Esc A v Eeprom Esc gt A y DelParam Esc F Table 10 Service For details please refer to chapter 9 7 Service Menu Issue 01 Page 29 7 4 Description of the key functions lt F1 gt lt F2 gt Function Keys Used to scroll through the different menus Depending on the menu other functions can be assigned to these keys The current function is always shown in the display right above the key lt gt lt gt These keys are used for numerical or text input and to select options from a list e g selection of the procedure to be started lt enter gt confirms the text numerical entry or selection and proceeds to the next dialogue element Issue 01 Page 30 8 Start up and system setup 8 1 Start Up Place the device on a suitable working surface Follow the steps described in the chapters 4 8 Installation requirements Environmental operating conditions and 4 9 Instrument installation procedure Connect power cable to standard mains plug e Switch on main switch rear left side The Atlantis performs the complete initialization and shows the main menu after approx 10 seconds Display Run Procedure lt lt gt gt Proci Screen 11 Display Description The first line of the display shows the name of the menu or the current fun
10. Overflow Dispense Height Teach in mode 9 6 In this teach in mode you can move the manifold to the correct height with the lt gt and keys Function keys F1 or F2 Select Y to automatically detect the bottom of the well lt gt Or lt gt Use these keys to move the manifold The manifold can be moved in steps of 0 1mm range 0 18 8mm lt enter gt Confirm the overflow dispense height The set parameters will be stored and the program returns to the main menu Setup Menu Please make sure to set all options according to your requirements Issue 01 Page 56 Setup lt lt gt gt Screen 75 Setup Menu Use the lt F1 gt lt F2 gt keys to select the setup menu Confirm the selection with lt enter gt Language Esc English Screen 76 Language Setup Use the lt gt lt gt keys to select the language and confirm with lt enter gt Manifold lt lt Esc 8needle Screen 77 Manifold Setup Use the lt gt lt gt keys to select the manifold and confirm with lt enter gt Wash liquids lt lt Esc Screen 78 Liquids Setup Use the lt gt lt gt keys to select the number of wash liquids connected to your instrument and confirm with lt enter gt Depending on the hardware configuration of your instrument you can use up to 3 wash liquids Issue 01 Page 57 Waste full det lt lt Esc Off Screen 79 Waste Setup Use
11. Page 51 lt gt Or lt gt Use these keys to enter the side position front The plate can be moved in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the side position front and go to the next screen Side pos rear Esc Mov 0 1 mm Screen 65 Side Position Rear Entry gt In this menu the side position rear for a flat bottom plate is defined It is only available if crosswise aspiration is enabled and it defines the second aspiration position The side position rear may be defined by teach in move the plate to the correct position with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes Select Mov to position the plate to the current side position rear and switch to the teach in mode lt gt Or lt gt Use these keys to enter the side position rear The plate can be moved in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the side position rear and go to the next screen Side pos rear Y Mov 0 1 mm Screen 66 Side Position Rear Teach in mode gt In this teach in mode the plate may be moved to the correct position with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Y to automatically detect the bottom of the well changes to qe Select T to move the manifold up Select Mov to move the plate transport to the curr
12. board software The display and the keyboard provides clear access to all features of the flexible software The Atlantis can be operated with up to 3 different wash liquids selected via the software An easy plate setup procedure is provided to optimize the movement of the plate and the manifold according to the different dimensions of different plate types Issue 01 Page 4 Warnings and Hazards gt General If inflammable toxic or biologically hazardous substances are used when operating the equipment please observe the instructions and precautions enclosed with such substance Never spill fluids in or on the equipment Wash your hands thoroughly after handling test fluids If equipment has been in contact with hazardous substances it must be disinfected prior to shipment in accordance with the effective provisions Do not touch the plate during movement of the plate transport risk of injury The equipment may not be opened other than as described in chapter 11 Maintenance since it contains live parts danger of life gt Electrical Voltages dangerous to human life are present in this device Before removing any covers disconnect the device from the power source Ensure that the power cord supplied with the unit is used The power cord may only be inserted in a socket outlet provided with a protective ground earth contact The protective action must not be negated by use of an extension cord without a protecti
13. gives the user the optimum choice to operate the Atlantis Software updates on the washer are performed conveniently from the computer Existing procedures or plate setups can easily be copied to another unit without the need to go through the programming again 4 5 Standard delivery content Washer Atlantis external tubing set e Manifold 8 or 12 way e lor3 wash bottles 21 depending on configuration Rinse bottle 21 Waste bottle 2 51 e Power cord e User manual e Spare fuses Cleaning tools for needles 4 6 Warnings and directions If inflammable toxic or biologically hazardous substances are used when operating the equipment please observe the instructions and precautions enclosed with such substances Issue 01 Page 11 Never spill fluids in or on the equipment Wash your hands thoroughly after handling test fluids If the equipment has been in contact with hazardous substances it must be disinfected prior to shipment in accordance with the effective provisions Do not touch the plate during movement of the plate transport risk of injury The equipment may not be opened other than as described in the chapter 11 Maintenance since it contains live parts danger to life Issue 01 Page 12 4 7 Unpacking Procedure 1 Visually inspect the container for damage before opening it Note Report any damage immediately to the forwarding agent or to the delivery carrier 2 3 4 5 Plac
14. of repeated liquid exchange down to a defined minimum rest volume for which the sample reagent liquid is aspirated from the wells into a waste bottle and the wash liquid is dispensed from stock wash bottles into the wells of the microplate the concentration of the sample shall be diluted until no diagnostically relevant rest remains The main field of use is ELISA testing operated and carried out by trained specialist stuff medical and or laboratory The Atlantis microplate washer has been designed to meet the 98 79 EC directive of the EU for in vitro diagnostic devices 12 1 Quality control The quality control criteria of a diagnostic kit have to be described in the package insert of the kit For the performance of any reagent quality control it has to be referred exclusively to those instructions Itis the user s responsibility to ensure proper operation according to the instructions given with each individual test Issue 01 Page 67
15. the lt gt lt gt keys to select whether a liquid level sensor is attached to the waste bottle and confirm with lt enter gt To use this function a liquid level detector must be connected to the instrument and the waste bottle If activated the liquid level of the waste bottle will be checked before the procedure is started In case that the level of the liquid in the waste bottle 1s above the sensor a corresponding warning will be displayed Rinse empty det lt lt Esc Off Screen 80 Rinse Setup Use the lt gt lt gt keys to select whether a liquid level sensor is attached to the rinse bottle and confirm with lt enter gt To use this function a liquid level detector must be connected to the instrument and the rinse bottle If activated the liquid level of the rinse bottle for a started procedure will be checked before the procedure is started In case that the level of the liquid in the rinse bottle is below the sensor a corresponding warning will be displayed Wash1 empty det lt lt Esc Off Screen 81 Washl Setup Use the lt gt lt gt keys to select whether a liquid level sensor is attached to the washl bottle and confirm with enter To use this function a liquid level detector must be connected to the instrument and the wash bottle If activated the liquid level of the washl bottle for a started procedure will be checked before the procedure is started In case that the level of
16. the instrument as shown on picture 3 Itis therefore recommended to save the original carton with its foam parts and accessories box Warranty claims are void if transport damages are caused by improper packing picture 1 picture 2 Issue 01 Page 16 5 1 5 2 5 3 5 3 1 5 3 2 Theory of Operation Introduction This chapter gives the description of the main components of the instrument and describes the hydraulic system Main components The main components of the washer are the control electronics the plate transport mechanism the manifold transport the dispensing and aspirating pumps the keyboard with the LCD display and the power supply unit Hydraulic system Dispensing system The dispensing system consists of the input selection valves the diaphragm pump the damping tube the pinch valve and the manifold The input selection valves are connecting the respective liquid reservoirs wash and rinse solution to the internal dispense circuits The valves are controlled by the washer software to select the correct liquid The dispensing pump delivers the liquid to the manifold the operating time of the pump together with the operating time of the pinch valve are controlling the programmed volume Aspirating system The aspiration is performed by a liquid pump connected to the aspiration needles of the manifold Compared to the traditional vacuum pump operation this method produces much less noise and does
17. Denville Atlantis Microplate Washer Users manual DENVILLE SCIENTIFIC INC Issue 01 Page 1 N 3 4 5 6 7 8 9 Li 3 1 4 1 4 1 1 4 1 1 4 2 4 2 1 4 2 2 4 2 3 4 2 4 4 3 4 4 4 5 4 6 4 7 4 8 4 9 4 9 1 4 10 5 1 5 2 5 3 5 3 1 9 32 6 1 7 1 7 2 7 3 7 3 1 7 3 2 7 3 3 7 3 4 7 3 5 7 3 6 7 3 7 74 8 1 8 2 9 1 9 2 9 2 1 Table of Contents Introduction do es o Ec Ec Warnings and Hazards ee le le dA AAA A A 6 AAA nana CA I Specifications Metrological sp cifications net ln no none Rated operating conditions Limits of error and tolerance OS cc s General technical data et ta nt DIM NSIONS enn a A ti POWer Supply EA EO I A ARE A is HA alo General Teatre AAA Standard delivery content Warnings and directions Unpacking Procedure ais Installation requirements Environmental operating conditions Instrument installation procedure cc Connecting the instrument with an external computer Re packing to prevent damage during transport Theory of Operation Introduction Main components Hydraulic system Dispensing system Aspirating SY StOM Een a esa moc A AMA nc ln one nn ne ne A IIA AA AA IO IA Software Principle SAN AA RS SE te Programming SCQuenCe nn i nue RUN Procedur dd A SERERE A SANS ss Define Procedure Define Cycle Define Plat
18. and to the waste bottle Connect the wash fluid tube with blue fitting to the connector at the backside of the washer and to the wash bottle Connect the rinse fluid tube with the white fitting to the connector at the backside of the washer and to the rinse bottle Ensure that the mains power switch at the back panel of the instrument is in the OFF position Insert the power cable into the mains power socket in the back panel of the instrument Switch the washer on If the instrument is turned on using the power switch on the rear side of the device the green power indicator on the left front side of the device has to be illuminated When connecting the instrument with an external computer make sure the instrument is switched off the power indicator is dark If the instrument is turned on turn it off using the power switch on the rear side of the device Issue 01 Page 14 Please Note The power indicator is located on the front left side of the unit and is marked with the following symbol O The stand by indicator may show 3 different states Green light Instrument is in stand by ready for operation Not illuminated Instrument is not ready for operation The power indicator does not indicate that the instrument is turned on or off or that it is connected with mains but only that the instrument is ready for operation In order to make sure the instrument is disconnected from power the main switch on the rear side of th
19. anifold Transport Error MF trans error Press any key Screen 98 Manifold Transport Error The manifold transport failed gt Function Keys lt enter gt Confirm the message Issue 01 Page 63 11 Maintenance 11 1 Recommended regular inspection The Atlantis is designed in a way that during appropriate use no danger for the user is caused Correct functional performance is assured by an automatic check of the relevant technical parameters during operation In case of a problem the instrument reports an error message Denville Scientific recommends to have the instrument inspected by an authorized service partner at an interval of about 2 years During this inspection the correct function of the instrument can be verified 11 2 Approved parts Except for the parts shown in the following list only parts supplied by Denville Scientific or an authorized Denville Scientific Distributor may be installed in or used with Denville Scientific Atlantis Fuses as specified in chapter 4 1 1 Rated operating conditions JPower cable CE marked power cable with connected protective earth and protective earth connector see also 4 8 Installation requirements Environmental operating conditions JExternal Computer controlling the instrument see also 4 9 1 Connecting the instrument with an external computer 11 3 Exchanging a Fuse In case of malfunction the green stand by indicator is not illum
20. ction of a sub menu The second line is split into 3 parts current function assigned to the first function key e g previous menu escape gt gt current function assigned to the second function key e g next menu escape Procl current selection or field for text or numerical input use the lt gt and lt gt keys to alter a selection or change a value 8 2 Control of the system setup Setup lt lt gt gt Screen 12 Setup Menu Please make sure to set all options according to your requirements before programming new procedures plates or cycles and before running procedures For details on this menu please refer to chapter 9 6 Setup Menu Issue 01 Page 31 9 Programming 9 1 Run Procedure Run Procedure lt lt gt gt Procl Screen 13 Run Procedure In this menu you can select the procedure to be started All procedures defined in the Define Procedure Menu can be selected gt Function keys lt F1 gt or lt F2 gt Select another menu lt gt Or lt gt Select a procedure to be started lt enter gt Confirm the selection of the procedure and go to the next screen Washliquid OK No Yes Screen 14 Washliquid Check A warning to check the liquid level of the wash bottle is displayed this function needs to be activated in the Setup Menu gt Function keys lt F1 gt or lt F2 gt Select Yes or No If No is selected the instrument wi
21. ctive earth connection carrying the CE mark The power outlet the instrument is connected to has to meet the applicable technical safety requirements Issue 01 Page 13 4 9 Instrument installation procedure The following procedures detail the necessary steps to be followed when installing the Instrument 1 Place the instrument into the required position Note Make sure the distance between the back panel of the instrument and the wall is at least 5 cm Remove all packing material and transport lock tapes 2 Remove all packing material from the manifold Look for the two manifold connecting tubes packed together with the manifold Connect the aspiration tube with the red quick fit connector to the corresponding connector at the top plate of the washer and slide the other end of the tubing on the red connector of the manifold Now connect the thin dispense tubing with the blue quick fit connector to the corresponding connector Press down now the black pinch valve actuator and slide the dispense tube into the side slot of the pinch valve Make sure that the tubing is fully inside the valve Afterwards slide the dispense tube on the blue connector of the manifold Place the manifold now on the horizontal manifold holder bar so that the two holding pins are resting in the slots of the manifold holder 3 4 T7 8 Connect the long aspiration tube with the red fitting to the connector at the backside of the washer
22. e Start up and system setup s dh Start U P ees or A a Sacalm cita Control of the system setup A A i Run Procedure tn 31 ACA M Manual Prime Rinse menmanna nnnm naera ae a aeee 34 O ci y 9 23 Penodic Prime RIJsex A ne ne etched 36 9 3 Define Pro ce dr Mt ten he ds Lt Br la 38 9 4 Define MA o 4 9 5 Define Plate Mense reete tette e tii 46 9 6 Setup Men US use e TE EE RR M AM t A E AT EL M A pas AE 56 9 7 Service Menus sell 60 10 ERROR MESSA CA aci 10 1 1 Duplicate A dd aci n l 10 1 2 Wrong Manol Le a e e MA D A a o l 10 1 3 No Cycle Define AAA el l 10 14 Li ta sl temes Sl 10 1 5 Waste Filles st 6 10 16 A A A fl 10 1 7 Stop Pressed AA ates ens NA A L 8 10 1 8 Plate Transport A 8 10 1 9 Manifold Transport Etforz i 8 11 INU ema a 1E T1 V6 a 11 1 Recommended regular inspection AN 64 11 2 Approved paris sh dl 64 11 3 Exchanging 4 EUse ucc ael el 64 11 47 Cleaning dhexnstr mentaszo soc as al Le 6 11 5 Disinfection Procedure ol 6 12 AN 12 1 AAA cel l Issue 01 Page 3 1 Introduction The Atlantis is a fully automated programmable 96 well microplate washer with 8 or 12 way manifolds An optional 16 channel manifold allows washing of 384 well microplates The Atlantis is designed around your application It incorporates state of the art technology and software both necessary to allow optimum performance and easy use Easy operation through the menu structure of the on
23. e 0 10 seconds lt enter gt Confirm the selection of the aspiration time and go to the next screen Aspir power lt lt Esc High Screen 46 Aspiration Power Entry gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to change the power with which the liquid is aspirated off a well Low Medium High lt enter gt Confirm the selection of the aspiration power and go to the next screen Disp volume lt lt Esc 1000 ul Screen 47 Dispense Volume Entry gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to change the dispense volume for this cycle in steps of 50ul range 0 2000u1 lt enter gt Confirm the selection of the volume and go to the next screen Disp power lt lt Esc High Screen 48 Dispenser Power Selection gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen Issue 01 Page 44 lt gt Or lt gt Use these keys to change the power pressure of the liquid for dispensing Low Medium High lt enter gt Confirm the selection of the dispens
24. e manifold can be moved in steps of 0 1mm range 0 18 8mm lt enter gt Confirm the aspiration height and go to the next screen Issue 01 Page 53 Disp pos Esc Mov 1 6 mm Screen 69 Dispense Position gt Disp pos For washing the dispensing needle is placed above the well From there 1t dispenses the washing solution into the well The aspiration needle aspirates an overflow and prevents liquid spilling on next wells The dispense position defines the position of the plate below the manifold during washing The dispensing needle should be above the well and the aspiration needle within It may be defined by teach in move the plate to the correct position with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes Select Mov to position the plate to the current dispense position and switch to the teach in mode lt gt Or lt gt Use these keys to enter the dispense position in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the dispense position and go to the next screen Mov 0 1 mm Screen 70 Dispense Position Teach in mode gt In this teach in mode the plate can be moved to the correct position with the lt gt and lt gt keys With Mov and J check if the needles are in correct position Function keys lt F1 gt or lt F2 gt Select Y to au
25. e the carton in an upright position and open it Remove the upper cardboard box carefully Lift the instrument out of the carton and place it on a flat surface free from dust vibration and away from direct sunlight Visually inspect the instrument for loose bent or broken parts Note Report any damage immediately 6 6 Te Compare the instrument s serial number attached on the rear panel of the instrument against the serial number of the instrument on the delivery shipping note and packing list Check the instrument accessories against the delivery shipping note and packing list Please save all packing materials as they maybe required for later transportation 4 8 Installation requirements Environmental operating conditions Please Note Flat dry clean and vibration proof working area 50 cm x 35 cm length x width Additional room for cables connections etc No direct sunlight Clean ambient air free of corrosive vapours smoke and dust Ambient temperature between 15 C and 40 C Humidity between 15 and 85 Before the instrument is installed and switched on it should be left to stand for at least 2 hours so there is no possibility of condensation causing a damage or malfunction Use the power cable supplied with the instrument to connect the instrument s main plug with a power outlet providing protective earth Only use the power cable supplied with the instrument or a power cable with prote
26. e unit has to be switched to the OFF position indicated with a 0 symbol Alternatively the power cord can be disconnected from the unit 4 9 1 Connecting the instrument with an external computer Please Note Turn the instrument off Connect the serial interface 9 pin DB9 connector on the computer with the connector at one end of the serial cable supplied by Denville Scientific Lock the connector to the plug using the corresponding screws on the connector Connect the other end of the serial cable with the plug marked RS232 on the rear side of the instrument Lock the connector to the plug using the corresponding screws on the connector Only use the original cable supplied with Atlantis Other cables with identical connectors may not be used to establish communication between Atlantis and a computer Issue 01 Page 15 4 10 Re packing to prevent damage during transport The original Atlantis packing has been specially designed for this equipment 1 Remove the manifold and tubing and the plate carriage and pack them into the accessories boxes 2 Lock the holder of the manifold with the foam part as shown in picture 1 3 Put the transport lock plate at the location shown in picture 2 and secure the plate with adhesive tape 4 Place the foam parts left and right on the washer and put the washer into the transport box picture 3 5 Place the the bottles and the accessory boxes into the spaces left and right of
27. e you can move the manifold to the correct height with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Y to automatically detect the bottom of the well lt gt Or lt gt Use these keys to move the manifold The manifold can be moved in steps of 0 1mm range 0 18 8mm lt enter gt Confirm the bottom dispense height and go to the next screen Ovflow disp ht Esc Mov 4 0mm Issue 01 Page 55 Screen 73 Overflow Dispensing Height gt It is recommended to use a dispense volume slightly higher than the well volume to produce an overflow phase The slight flow rate variations among dispensing needles are compensated by the overflow phase The overflow dispensing height defines the height of the manifold for overflow washing The position must be higher or equal as the aspiration height It may be defined by teach in move the manifold to the correct position with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes Select Mov to position the manifold to the current overflow dispense height and switch to the teach in mode lt gt Or lt gt Use these keys to enter the desired overflow dispense height in steps of 0 1mm range 0 18 8mm lt enter gt Confirm the overflow dispensing height and go to the next screen Ovflow disp ht 0 1 mm Y Screen 74
28. ent side position rear Issue 01 Page 52 lt gt Or lt gt Use these keys to enter the side position rear The plate can be moved in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the side position rear and go to the next screen Aspiration ht Esc Mov 7 0 mm Screen 67 Aspiration Height Position gt The aspiration height position defines the height of the manifold during aspirating a well It may be defined by teach in move the manifold to the correct position with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes Select Mov to position the manifold to the current aspiration height and switch to the teach in mode lt gt Or lt gt Use these keys to enter the aspiration height in steps of 0 1mm range 0 18 8mm lt enter gt Confirm the aspiration height and go to the next screen Aspiration ht y 0 1 mm Screen 68 Aspiration Height Teach in mode gt In this special teach in mode the manifold can be moved to the correct height with the lt gt and lt gt keys This mode features an automatic bottom detection For an optimal aspiration result move the aspiration needle about 0 5mm up Function keys lt F1 gt or lt F2 gt Select Y to automatically detect the bottom of the well lt gt Or lt gt Use these keys to move the manifold Th
29. es user exchangeable 2 pcs 2 5A TH 250V AC Built in Fuse on power supply 1 pes F 2A TH 250VAC e Display 2x16 characters display with backlight e Keyboard Keyboard with 5 function keys e Ambient temperature 15 C 40 C operation 25 C 50 C storage Relative humidity 15 8596 non condensing operation 95 non condensing storage e Air pressure tolerance 54 000 106 000 Pascal Maximum altitude operation up to 5000 m 4 1 1 Limits of error and tolerance limits Dispense volume Wash liquid volume adjustable from 50 2000ul in increments of SOul e Dispensing accuracy 5 at 300u1 across the plate Residual volume lul per well The tolerances of the measuring devices are based on the manufacturer s information 4 2 General technical data 4 2 1 Dimensions Weight 6 2 kg net e Dimensions width x length x height 21 cm x 46 cm x 21 cm 4 2 2 Power supply Consumption SOVA maximum Operating voltage 90 130V 180 250V AC autosensing frequency range 47 63 Hz Issue 01 Page 8 4 2 3 Interfacing e Serial interface RS232 9 pin male connector for remote control of basic wash functions instrument status and setup by external PC with null modem serial cable used for PC connection 4 2 4 Options liquid level sensors for wash and waste bottles e 8 and 12 way manifold for 96 well plates e 16 way manifold for 384 well plates Serial interface cable
30. he final aspiration Issue 01 Page 40 gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to enter the power for the final aspiration Low Medium High lt enter gt Confirm the final aspiration power and go to the next screen Processing mode lt lt ESC Plate Screen 38 Processing Mode Select the processing mode to be used for this procedure gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to scroll through the available modes Plate or Strip lt enter gt Confirm the selection of the processing mode and go to the next screen Screen 39 Plate Selection Select the plate to be used for this procedure The plate must be defined in the Define Plate Menu prior to the definition of the procedure gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to scroll through the available plates lt enter gt Confirm the selection of the plate and go to the next screen Ask strip nr lt lt Esc No Issue 01 Page 41 Screen 40 Ask for Last S
31. he procedure cycle or plate is already used gt Function keys lt enter gt Confirm the message 10 1 2 Wrong Manifold Wrong manifold Press any key Screen 91 Wrong Manifold The wrong manifold for the selected procedure is installed gt Function keys lt enter gt Confirm the message 10 1 3 No Cycle Defined No cycle defined Press any key Screen 92 No Cycle Defined There is no cycle defined gt Function keys lt enter gt Confirm the message 10 1 4 No Plate Defined No plate defined Press any key Screen 93 No Plate Defined There is no plate defined Issue 01 Page 61 gt Function keys lt enter gt Confirm the message 10 1 5 Waste Full Waste full Press any key Screen 94 Waste Full The waste is full Empty the waste bottle gt Function keys lt enter gt Confirm the message 10 1 6 Liquid Empty Liquid empty Press any key Screen 95 Liquid Empty The selected wash liquid is empty gt Function keys lt enter gt Confirm the message Issue 01 Page 62 10 1 7 Stop Pressed Stop pressed Press any key Screen 96 Stop Pressed The user has stopped the instrument gt Function keys lt enter gt Confirm the message 10 1 8 Plate Transport Error Platetrans error Press any key Screen 97 Plate Transport Error The plate transport failed gt Function keys lt enter gt Confirm the message 10 1 9 M
32. inated the fuses located in the mains inlet next to the mains socket on the rear side of the device can be checked and replaced if necessary e Disconnect the instrument from mains by unplugging the power cable e Open fuse carrier next to the mains socket with a screwdriver e Remove fuse e Insert spare included in supply 2 pcs 2 5 A TH250VAC e Close fuse carrier e Turn device on and check function In case of malfunction call a service technician Issue 01 Page 64 11 4 11 5 Cleaning the instrument This instrument is a precision instrument and the requires regular cleaning to ensure the continued precision Liquid spillage If any liquid is spilled on the instrument it should be IMMEDIATELY removed so that the liquid does not attack the surface of the housing Regular cleaning The housing of the instrument should be cleaned regularly with a mild household cleaning agent Warning Do not use aggressive solutions The area surrounding the plate carrier should be cleaned with a dry cloth Fittings and tubing The usual wash buffer tends to crystallize easily on air and can block tubing and fittings then Use the automatic rinse program daily and do not leave the tubing filled with wash buffer for an extended period of time Disinfection Procedure If the laboratory has no specific disinfection procedure the following procedure should be used to disinfect the instrument
33. ing power and go to the next screen Wash mode lt lt Esc Overflow Screen 49 Wash Mode Selection Two different wash modes are available Bottom the needles of the manifold are placed into the well to the defined position for the used plate during the wash procedure This means that basically only the bottom of the well is washed Overflow the needles of the manifold are placed to a certain position above the well to be also defined in the Define Plate Menu Therefore the whole well is thoroughly washed as the wash solution is only aspirated after it fills the well up to the defined position and reaches the aspiration needles of the manifold gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Shaking time lt lt Esc 5 Use these keys to toggle between Bottom and Overflow Screen 50 Shaking Time Entry gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to change to shaking time in steps of 1sec range 0 10 seconds Issue 01 Page 45 lt enter gt Confirm the selection of the shaking time and go to the next screen Shaking intens lt lt Esc Medium Screen 51 Shaking Intensity Entry gt Function ke
34. ld operate this instrument Alternating current VD In Vitro Diagnostic Device Protective ground terminal ON Caution see enclosed document gt o0 6e6lB The symbol on the product indicates that this product shall not be treated as household waste Instead it shall be handed over to the applicable collection point for the recycling of electrical and electronic equipment Issue 01 Page 6 3 1 Precautions Cleaning the instrument This instrument is a precision instrument and it requires regular cleaning to ensure the continued precision Liquid Spills If any liquid is spilled on the instrument it should be IMMEDIATELY removed so that the liquid does not attack the surface of the housing Regular cleaning The housing of the instrument should be cleaned regularly with a mild household cleaning agent Warning Do not use aggressive solutions The area surrounding the plate carrier should be cleaned with a dry cloth gt Fittings and tubing The usual wash buffer tends to crystallize easily on air and can then block tubing and fittings Use the automatic rinse program daily and do not leave the tubing filled with wash buffer for an extended period of time Issue 01 Page 7 4 Specifications 4 1 Metrological specification 4 1 1 Rated operating conditions e Warming up time Ready for operation immediately after switching on e Operating voltage 90V 130V 180V 250V autosensing e Fus
35. ll return to the main menu Waste empty No Yes Screen 15 Waste Check A warning to Ocheck the liquid level of the waste bottle is displayed this function needs to be activated in the Setup Menu Issue 01 Page 32 gt Function keys lt F1 gt or lt F2 gt Select Yes or No If No is selected the instrument will return to the main menu Warning If Yes is selected without a sufficient volume for waste liquid in the waste bottle the instrument can be damaged Plate inserted No Yes Screen 16 Plate Check A warning to check whether a plate is inserted is displayed this function needs to be activated in the Setup Menu gt Function keys lt F1 gt or lt F2 gt Select Yes or No If No is selected the instrument will return to the main menu Warning If Yes is selected without a plate inserted to the instrument spillage will be caused which can damage the instrument and or be harmful to the ambient depending on the used wash liquid Nr of strips Esc 12 Screen 17 Nr of stripes check The display prompts you to enter the right numbers of strips this function needs to be activated in the Setup Menu gt Function keys lt F1 gt Select Esc the instrument will return to the main menu lt gt lt gt Select number of strips lt enter gt Confirms the selection and starts procedure After confirmation of the above warnings the selected procedure wi
36. ll be started During the run the display will show the currently performed cycles Issue 01 Page 33 9 2 Prime Rinse Menu Prime rinse lt lt gt gt Manual Screen 18 Prime rinse Menu This menu allows to start a manual automatic or periodic prime rinse cycle s gt Function keys lt F1 gt or lt F2 gt Select another menu lt gt Or lt gt Select the desired mode for prime rinse Available options are manual automatic periodic lt enter gt Confirm the selection of the prime rinse mode and go to the next screen 9 2 1 Manual Prime Rinse Screen 19 Liquid Selection Select the liquid to be used for prime rinse gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt gt Or lt gt Select the desired liquid lt enter gt Confirm the selection of the liquid and go to the next screen Manual Pri Rinse ESC Go Screen 20 Manual Prime rinse The prime rinse can be started with the corresponding function key Go and will be performed as long as this key is pressed gt Function keys lt F1 gt or lt F2 gt Select esc to return to the main menu or Go to start the Issue 01 Page 34 prime rinse keep this key pressed as long as you like the prime rinse to be performed 9 2 2 Automatic Prime Rinse Liquid Esc Wash 1 Screen 21 Liquid Selection Select the liquid to be used for prime rinse gt Functio
37. n mode gt In this teach in mode the round bottom plate can be moved to the correct center position by using lt gt or lt gt Function keys lt F1 gt or lt F2 gt Select Y to automatically detect the bottom of the well changes to xiii s Select T to move the manifold up Select Mov to move the plate transport to the current center position lt gt Or lt gt Use these keys to enter the center position The plate can be moved in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the center position and go to the next screen Crosswise Aspir lt lt Esc Yes Screen 62 Crosswise Aspiration Selection If a flat bottom plate is selected this menu appears When using crosswise aspiration the aspiration needle is first moved close to one wall of the well side pos front and aspirates there After the defined aspiration time the needle is moved up to avoid scratching the bottom and is shifted to the opposite wall side pos rear There the needle is moved down and another aspiration sequence takes place For a better aspiration result crosswise aspiration is advisable Crosswise aspiration can only be used for flat bottom plates Issue 01 Page 50 gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to toggle between Yes and No l
38. n keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt gt Or lt gt Select the desired liquid lt enter gt Confirm the selection of the liquid and go to the next screen Screen 22 Volume Selection Select the liquid volume for prime rinse Issue 01 Page 35 gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt gt Or lt gt Select the desired volume in 1ml steps max 200ml lt enter gt Confirm the selection of the liquid volume and go to the next screen Start Pri Rinse Esc Screen 23 Start Prime Rinse gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt enter gt Press lt enter gt to start the prime rinse cycle with the above set parameters P R in process Stop Screen 24 Status Message gt Function keys lt F1 gt or lt F2 gt Select Stop to cancel the current prime rinse cycle and return to the main menu 9 2 3 Periodic Prime Rinse Screen 25 Liquid Selection Select the liquid to be used for prime rinse Issue 01 Page 36 gt Volume Esc Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt gt Or lt gt Select the desired liquid lt enter gt Confirm the selection of the liquid and go to the next screen Screen 26 Volume Selection gt Rinse Period Select the liquid volume for pe
39. not require tight closed bottles Any kind of container with sufficient volume can be used for the waste In addition the risk of infectious aerosols is much reduced Issue 01 Page 17 Diagram 1 Hydraulic Diagram Liquid Flow Diagram Inlet Solenoid Valves Liquid Connector Rinse Blue Manifold gt Dispense Wash1 p Liquid ump gt Connector Red Wash2 7 gt Aspirate Pump Wash3 gt To Waste Container Issue 01 Page 18 6 1 Warranty Liability Warranty shall lose effect if J Atlantis is not used in the defined scope of application J Atlantis has obviously been damaged by external influences which are not in accordance with the provisions for the nominal range of use J Atlantis has been modified or parts exchanged by a person other than Denville Scientific personnel or an authorized servicing agent Jthe warranty seals on the housing of the instrument are broken Jparts and subassemblies are implemented which are not original from Denville Scientific Jthe Atlantis serial number is no longer legible has been removed or altered JThe Atlantis has not been installed in accordance with the instructions supplied The Atlantis has been damaged during return transport due to wrong packing e g not in original packing material Jthe Atlantis was damaged due to wrong operation not according to the descriptions in the manual
40. riodic prime rinse Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt gt Or lt gt Select the desired volume in 1ml steps max 200ml lt enter gt Confirm the selection of the liquid volume and go to the next screen esc 10 min Screen 27 Rinse Period Selection gt Select the period of time after which the instrument is rinsed Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt gt Or lt gt Select the desired time period in steps of 1min max 200min lt enter gt Confirm the selection of the time and go to the next screen Start Pri Rinse esc Issue 01 Page 37 Screen 28 Start Prime Rinse gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu lt enter gt Press lt enter gt to start the prime rinse cycle with the above set parameters Periodic rinse Stop Screen 29 Status Message gt Function keys lt F1 gt or lt F2 gt Select Stop to cancel the current prime rinse cycle and return to the main menu 9 3 Define Procedure Menu Def Procedure lt lt gt gt lt new gt Screen 30 Define Procedure Menu In this menu a procedure can be defined Every procedure contains one or more cycles and a plate The cycles and the plate must be defined prior to the procedure gt Function keys lt F1 gt or lt F2 gt Select another menu lt gt
41. rn to the main menu without saving any changes or lt lt to return to the previous screen Issue 01 Page 39 lt gt Or lt gt Use these keys to scroll through the available cycles lt enter gt Confirm the selection of the first cycle and go to the next screen Screen 35 Cycle2 8 Selection Select the cycles 2 to 8 to be used for this procedure The cycles must be defined in the Define Cycle Menu prior to the definition of the procedure By selecting the previous cycle will be the last cycle in the procedure gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to scroll through the available cycles lt enter gt Confirm the selection of the cycle and go to the next screen Final asp time lt lt Esc sec Screen 36 Final Aspiration Time Entry Select the duration of the final aspiration gt Function Keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to enter the time for the final aspiration in steps of 1sec max 10 seconds lt enter gt Confirm the final aspiration time and go to the next screen Final asp speed lt lt Esc High Screen 37 Final Aspiration Power Entry Select the power for t
42. rocessing Esc Delay final asp mode Strip AND Final asp T time gt 0 lt ie 3 UN Storin a 9 For details please refer to chapter 9 3 Define Procedure Menu Issue 01 Page 25 7 3 4 Define Cycle Def Cycle aag gt A Y Esc Name pe m T lt La Esc Aspir time m p 4 T v P rs ed Esc Aspir power if Aspir time 0 lee Esc Disp volume b P m T lt v Legs Esc Disp power e pp a if Disp El volume 2 0 Lee Esc Wash mode R t_ lt aed Esc 4 Shaking time T 8 t_ lt Shaking H lt if Shaking ees intensity lg time 0 y Minimum soak r t Esc time i v y dE URS Storing Pu P Table 7 Define Cycle For details please refer to chapter 9 4 Define Cycle Menu Issue 01 Page 26 7 3 5 Define Plate 4 gt gt gt Def Plate 1 v Esc Name t_ lt v PERS a Esc Plate format M lt v Esc Well shape lt Flat i Round lt lt 4 Esc Crosswise asp T 4 a dl Esc Center pos dl Esc Side pos front T lt v If Cross M4 Esc
43. s displayed prior to the start of a procedure Waste warning lt lt Esc On Yes Screen 85 Waste Warning Setup Issue 01 Page 59 Use the lt gt lt gt Keys to activate deactivate the waste warning and confirm with lt enter gt If activated a reminder to check the level of the waste bottle is displayed prior to the start of a procedure Plate warning lt lt Esc On Yes Screen 86 Plate Warning Setup Use the lt gt lt gt keys to activate deactivate the plate warning and confirm with lt enter gt Keyclick lt lt Esc Screen 87 Keyclick Setup Use the lt gt lt gt keys to activate deactivate the keyclick and confirm with lt enter gt Emergencystop lt lt Esc On Screen 88 Emergencystop Setup Use the lt gt lt gt keys to activate deactivate the emergencystop and confirm with lt enter gt If a strip of a microplate or the microplate itself is not inserted correctly the tips of the manifold will hit the bottom This triggers the bottom detection sensor and every process will be stopped immediately 9 7 Service Menu Service lt lt gt gt Sensors Screen 89 Service Menu This menu allows to check various functions of the instrument It is only intended to be used by trained service personnel Issue 01 Page 60 10 ERROR MESSAGES 10 1 1 Duplicate Name Duplicate name Press any key Screen 90 Duplicate Name The name for t
44. s optimization of plate specific parameters The instrument is able to automatically detect the bottom of the well This results in improved washing performance and minimized residual volume without the manifold touching the bottom of the well with flat bottom plates only Wash cycles and procedures Wash cycles Up to 20 may be defined according to specific requirements and are stored on the instrument Once a wash cycle is created it may be used in different wash procedures Issue 01 Page 10 Some of the main features are Bottom and Overflow washing Adjustable Dispense volume and speed Adjustable aspiration power and time Adjustable soak time Shaking 3 modes Wash procedures Up to 50 may be created by putting up to 8 previously defined wash cycles in sequence Washing procedures can either be performed step by step over the whole plate PLATE mode or strip by strip STRIP mode The number of strips to be processed may be defined at the time the wash procedure is started Options Liquid level detectors to check volumes in wash and waste bottles Remote control software for PC Windows based software for programming of washing procedures and plate configurations The washer may be remotely controlled from the PC to run the instrument in a Windows environment Data may be transferred between instrument and PC The combination of both platforms the flexible onboard software and the convenient Windows based program
45. t enter gt Confirm the selection for crosswise aspiration and go to the next screen Side pos front Esc Mov 0 1 mm Screen 63 Side Position Front Entry gt In this menu the side position front for a flat bottom plate is defined If you choose crosswise aspiration this is the first aspiration position Else this is the aspiration position for a better aspiration result it is recommended to position the needle close to one wall The side position front may be defined by teach in move the plate to the correct position with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes Select Mov to position the plate to the current side position front and switch to the teach in mode lt gt or lt gt Use these keys to enter the side position front The plate can be moved in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the side position front and go to the next screen Side pos front Y Mov 0 1 mm Screen 64 Side Position Front Teach in mode gt In this teach in you can move the plate to the correct position with the lt gt and lt gt keys Function keys lt F1 gt or lt F2 gt Select Y to automatically detect the bottom of the well changes to eos Select T to move the manifold up Select Mov to move the plate transport to the current side position front Issue 01
46. the liquid in the wash1 bottle is below the sensor a corresponding warning will be displayed Wash2 empty det Esc Off Issue 01 Page 58 Screen 82 Wash2 Setup Use the lt gt lt gt keys to select whether a liquid level sensor is attached to the wash2 bottle and confirm with lt enter gt To use this function a liquid level detector must be connected to the instrument and the wash2 bottle If activated the liquid level of the wash2 bottle for a started procedure will be checked before the procedure is started In case that the level of the liquid in the wash2 bottle is below the sensor a corresponding warning will be displayed Wash3 empty det lt lt Esc Off Screen 83 Wash3 Setup Use the lt gt lt gt keys to select whether a liquid level sensor is attached to the wash3 bottle and confirm with lt enter gt To use this function a liquid level detector must be connected to the instrument and the wash3 bottle If activated the liquid level of the wash3 bottle for a started procedure will be checked before the procedure is started In case that the level of the liquid in the wash3 bottle is below the sensor a corresponding warning will be displayed Liquid warning lt lt Esc On Screen 84 Liquid Warning Setup Use the lt gt lt gt keys to activate deactivate the liquid warning and confirm with lt enter gt If activated a reminder to check the level of the wash bottle s i
47. tomatically detect the bottom of the well changes to y Select T to move the manifold up Select Mov to move the plate transport to the current dispense position lt gt Or lt gt Use these keys to enter the dispense position The plate can be moved in steps of 0 1mm range 5 0 5 0mm lt enter gt Confirm the dispense position and go to the next screen Issue 01 Page 54 Bottom disp ht Esc Mov 0 0mm Screen 71 Bottom Dispensing Position For additional bottom washing the needles are moved upwards to Bottom dispensing height and then down again to the aspiration height The bottom dispensing height defines the vertical position of the manifold during dispensing of a bottom wash cycle The dispensing height must be higher or equal the aspiration height It may be defined by teach in move the manifold to the correct position with the lt gt and lt gt keys Function keys F1 or F2 Select Esc to return to the main menu without saving any changes Select Mov to position the manifold to the current bottom dispense height and switch to the teach in mode lt gt Or lt gt Use these keys to enter the dispense height in steps of 0 1mm range 0 18 8mm lt enter gt Confirm the bottom dispensing position and go to the next screen Bottom disp ht 4 0 1 mm Screen 72 Bottom Dispense Height Teach in mode gt In this teach in mod
48. trip Select Yes if the selection of a number of strips should be possible every time this procedure is started This can be useful if for example full plates and plates with just a few strips are used subsequently with the same procedure gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to toggle between Yes and No If Yes is selected you can define the last strip every time the procedure is started No means that every time all strips of a plate are washed lt enter gt Confirm the selection and go to the next screen Delay final asp lt lt Esc Yes Screen 41 Delay of Final Aspiration Select Yes to delay the final aspiration of the plate This is used in strip mode The final aspiration is performed only after the last cycle in the last filled strip is done and the soak time has expired gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to toggle between Yes and No If Yes is selected the final aspiration is performed after the last cycle for all strips is finished lt enter gt Confirm the selection The set parameters will be stored and the program returns to the main menu 9 4 Define Cycle Menu
49. trips the corresponding manifold is used to wash a selected number of strips or the complete plate Up to 3 different wash liquids The basic version incorporates 1 liquid line for wash buffer rinse liquid and waste The extended version accesses to up to 3 different wash buffers connected to the instrument which can be selected by software Vacuum and pressure free system Any appropriate container can be used to hold the wash buffer No special bottles are required Quiet operation The Atlantis is very quiet due to the special pumps used Additionally the pumps are only operating when the washing procedure is performed Automatic periodic rinse May be activated in order to avoid clogging if the washer is not being operated Aerosol Protection Covers the manifold and the rear plate position It can easily be removed for cleaning and maintenance Easy access to all tubes and valves All valves and tubes which may require handling by the user are easily accessible to assure fast and convenient maintenance Plate setup Is used to optimize the movement of the plate and manifold according to the specific dimensions of different plate types Residual volume is minimized by the crosswise aspirate function which aspirates at the edges of the well Up to 20 different plate types flat and round bottom can be configured and stored on the instrument Bottom Detection As part of the plate setup this unique feature support
50. ttom and curved bottom plates Issue 01 Page 47 Def Plate lt lt gt gt lt new gt Screen 55 Define Plate Menu gt This menu allows to define all parameters for a plate A defined plate is required to for a complete wash procedure Function keys lt F1 gt or lt F2 gt Select another menu lt gt Or lt gt Select lt new gt to define a new plate or select an existing plate to edit it lt enter gt Confirm the selection of the plate and go to the next screen Screen 56 Plate Name Entry gt Name Esc Sel The name of the plate may be changed Function keys lt F1 gt or lt F2 gt Select Esc to return to main menu without saving any changes Select Chg to change the name for the plate lt enter gt Confirm the existing name and go to the next menu Screen 57 Plate Name Entry gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or Sel to select the current letter number lt gt Or lt gt Use these keys to scroll through the available letters A Z and numbers 0 9 lt enter gt Confirm the name of the plate and go to the next screen Issue 01 Page 48 Plate format lt lt Esc 8 strip Screen 58 Plate Format Selection gt Function keys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen
51. up to 8 cycles Up to 50 procedures can be stored NOC SI to 20 cycles can be stored Drawing 3 Software Principle Note Please make sure to program the cycle and plate BEFORE the procedure Issue 01 Page 21 J 3 Menus Run Procedure Proci lt lt gt gt 1 Prime Rinse lt lt gt gt Automat y 1 Def Procedure lt lt gt gt 4 Def Cycle lt lt gt gt 4 Def Plate lt lt gt gt 1 Setup lt lt gt gt i f Service lt lt gt gt d new new new The following flow diagrams give an overview of all menus and the way of navigating through them Detailed information for all menus and their functions can be found in the following chapters For details please refer to chapter 9 1 Run Procedure For details please refer to chapter 9 2 Prime Rinse Menu For details please refer to chapter 9 3 Define Procedure Menu For details please refer to chapter 9 4 Define Cycle Menu For details please refer to chapter 9 5 Define Plate Menu For details please refer to chapter 9 6 Setup Menu For details please refer to chapter 9 7 Service Menu Issue 01 Page 22 7 3 1 Run Procedure 34 Run gt gt gt Procedure eat 4 v lt Esc Nr of strips if Ask strip nr No a No Washliquid OK if disabled Yes j a No Waste empty if disabled
52. ve grounding contact Do not replace fuses without first removing the main power cord Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuse holders is prohibited When the apparatus is connected to the main power source the opening of the covers or removal of components is likely to expose life parts The device shall be disconnected from all voltage sources before it is opened for adjustment or repair Any adjustment or repair of the opened apparatus under voltage should be avoided but if necessary it must be carried out by qualified service personnel who are aware of the hazards involved Issue 01 Page 5 gt Magnetic e The removable plate carriage of this instrument has a very strong permanent magnet mounted Be sure to never bring any other magnets or Ferro magnetic parts near to the magnet of the plate carriage risk of injury e Never place credit cards or IDs or any other items using magnet code floppy disks audio or video tapes near to the magnet of the plate carriage risk of data loss e Becareful when placing the plate carriage onto other sensitive medical devices which may be influenced by magnetic fields 2 1 1 Safety symbols The following safety symbols may be found in several locations on the instrument Only persons who fully understand the safety precautions and recognize shock hazards shou
53. ys lt F1 gt or lt F2 gt Select Esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to change the intensity for shaking Low Medium High lt enter gt Confirm the selection of the shaking intensity and go to the next screen Min soak time lt lt Esc 10 sec Screen 52 Minimum Soaking Time Entry The minimum soaking time defines the time which must elapse at least between dispensing and aspirating a well gt Function keys lt F1 gt or lt F2 gt Select esc to return to the main menu without saving any changes or lt lt to return to the previous screen lt gt Or lt gt Use these keys to change the minimum soaking time in steps of 10sec range 0 1000 seconds lt enter gt Confirm the selection of the minimum soaking time The set parameters will be stored and the program returns to the main menu 9 5 Define Plate Menu The following drawings help to find the correct positions of the manifold and plate Please note that curved bottom plates do not allow crosswise aspiration Dispense needle Aspiration needle Dispense needle Aspiration needle Issue 01 Page 46 I Center pos Ll I Horizontal 0 Position Dispense needle Aspiration needle Vertical 0 Position Horizontal 0 Position i Horizontal 0 position i Drawing 54 Wash Positions for flat bo
54. ys function key 1 F1 and function key 2 lt F2 gt When pressing F1 or F2 the next respectively the previous menu will be displayed The two function keys may have different functions assigned in the various sub menus The current assignment is always displayed right above the corresponding key The lower right of the display will show the current selection of different options e g Procl for the procedure with this name To change the selection use the lt gt and lt gt keys These keys can also be used for numerical or alphanumerical inputs by means of in or decreasing a value or scrolling through the alphabet By pressing enter the current selection is confirmed and the software leads to the next menu to allow further selections Issue 01 Page 20 7 2 Programming Sequence The software offers the possibility to program up to 50 so called procedures a procedure defines a complete wash program To define a procedure cycles defines one part cycle of a wash program e g bottom wash and plates defines the shape and measures of the plate used are necessary Aie zy riate 1 Plate defines the shape of a plate and the relative Positions of plate and manifold Up Procedure 0 to 20 plates can be stored Cycle 20 ae Y Cycle defines the washing methode Different ways can be selected eg bottom wash aspiration J Up Procedure defines tha plate and

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