User manual OR 100
Contents
1. 5 If ordered position the AS 100 autosampler and DECADE II LC EC workstation at the right side of the OR 100 with a distance between the instruments of a few centimetres Align the back panels of the equipment as depicted in Fig 5 OR 100 AS 100 DECADE II Fig 5 Top view of the ALEXYS LC EC system schematic Circle indi cates location of OR 100 drain on mobile phase tray CHAPTER 2 Installation guide 15 6 Verify that the silicone tubing is connected which is in the ship kit to the drain of the mobile phase tray at the back side of the OR 100 110 to prevent leakage of solvents onto the electrical equipment below LC 100 pump or AC 100 acquisition controller 7 Connect Teflon tubing part 180 0204 with solvent filter to make a low pressure connection between mobile phase reservoir and degasser 8 Connect tubing part 180 0206 from degasser to pump inlet see Fig 6 Only use supplied nut ferrule set part 180 1550 180 1552 pro vided in the ship kit to connect tubing to degasser 9 Connect 0 02 ID stainless steel tubing part 180 0210 to make a high pressure connection between pump and pulse damper Only use sup plied nut ferrule set for pump part 182 0404 182 0406 pulse damper part 180 1554 180 1556 Further details on installation of all liquid connections are in user manual of the LC connection kit Filter Mobile phase Pump inlet Injector Pump outlet Pulse damper Fig 6 LC connect2. Corrosive solvent precautions 33 System shutdown 33 Trouble shooting guide 35 All ALEXYS system components are off 35 Degasser LEDs are off 35 Degasser yellow status LED is on or flashing 35 Air bubbles in FEP PTFE tubing 35 No solvent flow through degasser 36 Regular baseline oscillations 36 Leakage of mobile phase via OR 100 drain bottom plate 36 Specifications ALEXYS OR 100 110 Organiser Rack 37 Accessories 38 Index 39 10 User manual OR 100 110 edition 4 CHAPTER 1 OR 100 110 organiser rack Congratulations on your purchase of the OR 100 or 110 organiser rack OR 100 The OR 100 is a multi functional organiser rack for the ALEXYS 100 LC EC system It is used to accommodate one or two LC 100 pumps an AC 100 acquisition controller and mobile phase bottles and is equipped with an integrated two channel degasser and pulse damper 2 dampers op tional Moreover it has a power divider on the back panel to supply up to 8 external devices All ALEXYS system components such as pump s autosampler and DECADE II can be fed via the OR 100 110 power divider so only a single power cord is used to connect a complete ALEXYS LC EC system to a mains outlet This construction prevents generation of ground loops and ensures optimum performance of the ALEXYS 100 LC EC sys tem See figure 1 and cover page of this manual for a photograph of the OR 100 Fig 1 Front view of the ALEXYS 100 LC EC system with OR 100 organ iser rack L
3. disposed as unsorted municipality waste Collection amp recycling information Please ship the instrument back to the manufacturer Antec Leyden the Netherlands at the end of life time of the product The manufac turer will take care of the proper disposal and recycling of the in strument at its facilities Shipping address for the end of life products Antec Leyden Industrieweg 12 2382NV Zoeterwoude The Netherlands In case of questions or if further information is required about the collec tion amp recycling procedure please contact your local distributor ROHS directive Our instruments are currently exempt from the RoHS directive because they fall under WEEE Annex IA categories 8 and 9 which includes medi cal devices and monitoring and control instruments Nevertheless we have taken steps to eliminate all restricted substances from our products ISO 9001 2000 Q e A Antec Leyden is an ISO 9001 2000 certified company A QUALITYMASTERS iv User manual OR 100 110 edition 4 Symbols The following symbols are used on the rear panel and oven compartment of the OR 100 f Consult the manual for further safety instructions Frame or chassis ground terminal The following pictograms are used in the OR 100 110 manual f Caution Caution risk of electric shock or other electrical hazard high voltage Introduction Safety practices A AN The following safety practices are intended to ensure
4. on the degasser the microprocessor examines the vacuum sensor signal to confirm that it is within an expected range Following the start up test the microprocessor ramps the vacuum pump to 30 User manual OR 100 110 edition 4 high RPM to quickly exhaust atmosphere from the vacuum chamber As the vacuum level approaches the preset control value the pump will slowly ramp down to a low speed typically 40 to 60 RPM and will vary slightly as needed under the changing degassing load to maintain a virtually constant vacuum level During initial pump down the yellow Status LED will be lit Once the vac uum has reached normal operating level the yellow LED will extinguish and the green Vacuum LED will illuminate If you want to confirm that the pump is running beyond the front panel LEDs the slight vibration caused by the micro stepping of the motor driving the vacuum pump may be felt by placing your hand on the instrument By varying the speed of the vacuum pump the degasser maintains a con stant vacuum pressure of 50mm Hg absolute nominal depending on the degassing load in the system The pump is designed for at least 5 years of constant running and has integral in pump venting which eliminates the need for stop start running If a potential leak is detected if the pump RPM gt 300 for 30 minutes the pump will be shut down and the yellow Status LED will flash The vacuum is maintained as long as the degasser is pow ered on Solve
5. pump and monitored by the microprocessor through an inte grated absolute pressure sensor Degassed mobile phase exits the vac uum degasser and enters the pump Pressure Sensor HPLC pump Channel 1 Channel 2 voie G C Vacuum Phase C Pump Vacuum chamber Fig 12 Block diagram of OR 100 degasser Principles of degassing using Teflon AF membranes The fully amorphous nature of Teflon AF has a molecular level porosity superior to the mechanically induced porosity in extruded PTFE tubing in many other devices The very small surface area required to degas the mobile phase reduces the carryover from one solvent to another to virtu ally zero Teflon AF is inert to all solvents normally used in HPLC How ever Teflon AF is soluble in perfluorinated solvents such as Fluorinert FC 75 and FC 40 and Fomblin perfluoro polyether solvents Use of such Chapter 4 Operation 29 solvents in the degasser will result in dissolution and destruction of the membrane Teflon AF is permeable to some degree to water vapour whereas PTFE is not While the vacuum pump in the degasser contains internal provi sions for sweeping water or solvent vapour from the pump continuously it is possible that over time high concentration buffers may form crystals within the channel due to the loss of water within the channel Therefore Flush system with pure water before switching off OR 100 110 for longer period of time days as precipitation o
6. safe operation of the equipment Electrical hazards The removal of protective panels on the OR 100 110 can result in expo sure to potentially dangerous voltages Therefore disconnect the instru ment from all power sources before disassembly Unqualified personnel should not open the instrument V 100 240 FUSE RATING POWER OUTLETS max 1900 VA 240 VAC 50 60Hz max 80 VA 100 VAC BAT 250V MAX 3OOVA ouruer WARNING RISK OF FIRE WARNING RISK OF ELECTRIC SHOCK REPLACE FUSE AS MARKED CISCONNECT POWER BEFORE SERVICING AVERTISSEMENT RISQUE DE FEU AVERTISSEMENT RISQUE DE CHOC ELECTRIQUE REMPLACEZ LE PUSIBLE COMME NDIQUE COUPER L ALIMENTATION AVANT LA MAINTENANCE Replace blown fuses with fuses of proper type and rating as stipulated on the side panel and specified in the installation section of this manual The fuse holder is integrated in the mains connector Ensure that the instru ment is never put in operation with fuses of a different type This can cause fire Connect the detector to a grounded AC power source line voltage 100 240 VAC frequency 50 60 Hz The instrument should be connected to a protective earth via a ground socket The power source should exhibit minimal power transients and fluctuations Replace faulty or frayed power cords vi User manual OR 100 110 edition 4 The OR 100 110 is equipped with 8 AC power outlets at the back panel divided in two blocks of 4 The maxim
7. to make the connection on the low pressure side so from MP bottle gt degasser and degasser gt pump inlet Only use the supplied nut ferrule set part 180 1550 180 1552 provided in the ship kit to connect FEP tub ing to degasser For the connections on the high pressure side stan dard 10 32 PEEK Fingertights can be used 18 User manual OR 100 110 edition 4 Electrical connections OR 100 In this section the electrical connections to the OR 100 are described for an ALEXYS 100 system based on the LC 100 pump and AC 100 control ler For the OR 110 skip this part and continue with the section Electrical connections OR 110 For a complete installation procedure of an ALEXYS LC EC system and its components consult the individual user manuals pump autosampler etc and the installation guide provided in the ALEXYS connection kit Mains connections OR 100 does not have a power switch Plugging in power cord to mains immediately powers up all connected devices Before connecting power cords check fuse rating and mains power specifications of ALL devices See user manuals for details e Connect the 0 6m power cords p n 250 0140 to the LC 100 AC 100 and AS 100 and socket 4 6 on the OR 100 see Fig 9 e Connect the 1 8m power cord p n 250 0141 to the DECADE II and socket 3 e Connect the 5m power cord p n 250 0143 to the PC and socket 2 Fig 9 Back view of the OR 100 with LC 100 pump and AC 100 Connec tors
8. 1 10 are numbered for reference CHAPTER 2 Installation guide 19 A N Do NOT connect OR 100 to mains power at this point yet Table Il Connection scheme OR 100 power cables Outlet no Part no Connect to 1 250 0116 Mains 2 250 0143 Personal Computer 3 250 0141 DECADE II detector 4 250 0140 AS 100 autosampler 5 250 0140 LC 100 pump 6 250 0140 AC 100 acquisition controller Use the manufacturer supplied power cable s only to connect all other devices with the OR 100 Manufacturer will not accept any li ability for damage direct or indirect caused by connecting this in strument to devices which do not meet relevant safety standards Serial connections Every individual ALEXYS system component is shipped with a specific RS232C cable for computer control These cables can be found in the corresponding ship kits The part numbers are listed in the table below Table Ill Serial cables of ALEXYS 100 LC EC system components Part no Description Qty 250 0122 DECADE II RS232 cable 181 0596 AS 100 serial cable 9M 9F pins 182 0544 LC 100 serial cable 9F 25F pins 183 0502 AC 100 serial cable 9F 9F pins lt A a es e Connect serial cables specified in Table IV in corresponding ports of the AC 100 See Fig 9 for port numbers Use always D A P DECADE Il Autosampler Pump order of connect ing devices to be compatible with pre configured device drivers in ALEXYS data system 20 User manual OR 100 110 e
9. 10 and related liquid and electrical connections only For a complete installa tion procedure of ALEXYS LC EC system consult the user manual of all system components pump autosampler etc and the installation manual provided in the ALEXYS connection kit For installation of the LC connections on the OR 100 110 the appro priate LC connection kit is required containing a complete set of tubing and fittings Unpacking of OR 100 110 Unpack the OR 100 100 in the following way Lift it from its box by both hands as illustrated in Fig 3 and put it on a suitable location meeting specifications as described in the section operating environment Lift Lift Drain Drain here Fig 3 Lift instruction OR 100 organiser rack left side and OR 110 right side User manual OR 100 110 edition 4 Installation of OR 100 The installation procedure of the OR 100 consists of the following steps 1 Remove the aluminium covers in front of the pulse damper and de gasser to avoid scratches on pump and controller during installation Gently pull on the covers until they release snap in connectors 2 Put the LC 100 pump into the OR 100 organiser rack with its feet into the holes of the alignment plate as depicted in Fig 4 3 Stack the AC 100 acquisition controller on top of the LC 100 pump see Fig 5 LC 100 HPLC pump Alignment plate Remove aluminium front panel Fig 4 Stacking LC 100 pump on OR 100 organiser rack
10. C 100 pump AC 100 controller and AS 100 autosampler Chapter 1 OR 100 110 organiser rack 11 OR 110 The OR 110 is a multi functional organiser rack for the ALEXYS systems based on the LC 110 pump It is equipped with an integrated two channel degasser and pulse damper Moreover it has a power divider on the back panel to supply up to 8 external devices All ALEXYS system components such as pump s autosampler and DECADE II can be fed via the OR 100 110 power divider so only a single power cord is used to connect a complete ALEXYS LC EC system to a mains outlet This construction pre vents generation of ground loops and ensures optimum performance of the ALEXYS LC EC system Fig 2 Front view of OR 110 organiser rack top ALEXYS LC EC system with LC 110 pumps 12 User manual OR 100 110 edition 4 CHAPTER 2 Installation guide Unpacking Inspect the transport box for possible damage as it arrives Immediately inform the transport company in case of damage otherwise she may not accept any responsibility Keep the transport box as it is designed for op timum protection during transport and it may be needed again Carefully unpack the instrument and inspect it for completeness and possible dam age Contact your supplier in case of damage or if not all marked items on the checklist are included Prior to shipment your OR 100 110 has been inspected and tested to ensure the best possible performance The results of these test
11. Proven Performance Antec Antec ALEXYS OR 100 110 Organiser rack user manual 184 0010 Edition 4 2009 Antec Leyden bv Industrieweg 12 2382 NV Zoeterwoude The Netherlands Tel 31 71 5813333 e fax 31 71 5813334 e mailbox myantec com e www myantec com Introduction Copyright 2008 Antec Leyden All rights reserved Contents of this publication may not be reproduced in any form or by any means including electronic storage and retrieval or transla tion into a foreign language without prior agreement and written consent from the copyright of the owner The information contained in this document is subject to change without notice DECADE II is a trademark and ALEXYS a registered trademark of Antec Leyden BV Whatman word and device and Whatrnan word only are trademarks of Whatman International Ltd SOLVENT IFD and AQUEOUS IFD are trademarks of Arbor Technolo gies Inc Clarity DataApex are trademarks of DataApex Ltd Microsoft and Windows are trademarks of Microsoft Corporation The information provided herein is believed to be reliable Antec Leyden shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this manual All use of the hardware or software shall be entirely at the user s own risk ii User manual OR 100 110 edition 4 DECLARATION OF CONFORMITY The manufacturer h
12. cal normal operation see Powering up the degasser page 29 System leak or fault Contact your local supplier for service Air bubbles in FEP PTFE tubing Possible cause Loose fittings Mixing of solvents requires more capacity of degasser Flow rate too high Degasser not functioning Damaged PTFE tubing Remedy Check and if needed tighten the input and output 1 8 fittings on the solvent ports Connect both channels to double capacity Connect both channels to double capacity Switch on degasser Replace FEP PTFE tubing 36 User manual OR 100 110 edition 4 No solvent flow through degasser Possible cause Remedy Air in pump head Prime purge the pump head Blocked 0 2 um in line filter Replace filter Blocked degasser solvent Use a different degasser channel or port caused by unfiltered or _ flush back flush the channel with water or buffered mobile phases other appropriate solvent MeOH iso propanol Note draw the solvent through the channel to dissolve salts or remove particles Regular baseline oscillations Possible cause Remedy Malfunctioning pump regular Check pump seals valves pattern Back pressure too low for For optimal dampening of flow pulsations a pulse damper backpressure of at least 7 MPa 70 bar 1000 psi is needed Use backpressure regulator or restriction capillary between pulsdemper and injector to increase pres sure Pulse damper defect internal Contact your local supplier for se
13. dition 4 Table IV Connection scheme RS232 cables on AC 100 Port Part no Connect to PC 7 183 0502 Personal computer 1 5 8 250 0122 DECADE II 2 6 9 181 0596 AS 100 autosampler 3 7 10 182 0544 LC 100 pump Please refer to the manuals of the individual system components LC 100 AC 100 AS 100 and DECADE Il for specific installation instructions These manuals contain important information about the installation main tenance and safety practices Individual ship kits of ALEXYS 100 components have a small overlap of parts with ALEXYS installation kit Therefore In case of a complete ALEXYS 100 system installation a few super fluous parts in the kit are left over Electrical connections OR 110 In this section the electrical connections to the OR 110 are described for an ALEXYS system based on the LC 110 pump Such system is delivered without AC 100 controller For the OR 100 refer to the previous section Electrical connections OR 100 For a complete installation procedure of an ALEXYS LC EC system and its components consult the individual user manuals pump autosampler etc and the installation guide provided in the ALEXYS connection kit Mains connections OR 110 does not have a power switch Plugging in power cord to mains immediately powers up all connected devices Before connecting power cords check fuse rating and mains power specifications of ALL devices See user manuals for details e Connec
14. duction run of the instrument Spare parts will be available after this five years period on an as available basis Manufacturer provides a variety of services to support her customers after warranty expiration Repair service can be provided on a time and material basis Contact your local supplier for servicing Technical support and training can be provided by qualified chemists on both contractual and as needed basis viii User manual OR 100 110 edition 4 Table of contents DECLARATION OF CONFORMITY ii Intended use iii WEEE directive iii ROHS directive iii Symbols iv Safety practices v Electrical hazards v General precautions vii Spare parts and service availability vii OR 100 110 organiser rack 10 Installation guide 12 Unpacking 12 Operating environment 12 Installation liquid connections 13 Electrical connections OR 100 18 Mains connections 18 Serial connections 19 Electrical connections OR 110 20 Mains connections 20 Serial connections 21 Powering up system 23 Description of instrument 25 Front side 25 Back side 27 Operation 28 OR 100 110 integrated dual channel degasser 28 Principle of Operation 28 Principles of degassing using Teflon AF membranes 28 Start Up Test and Pump Operation 29 Smart Leak Detection 29 Powering up the degasser 29 Extending the degassing flow rate range 30 OR 100 110 integrated pulse damper 31 Principle of operation 31 Maintenance 32 Precautions and system shutdown 32
15. e drain on the bottom of the mobile phase tray is not blocked or closed Do not allow flammable and or toxic solvents to accumulate Fol low a regulated approved waste disposal program Empty and clean waste container regularly Never dispose of such products through the municipal sewage system Clean the OR 100 110 external surfaces on a regular basis Use a cloth wetted with water only to clean the detector Do not use any organic solvents to clean the exterior Use only high purity gases when drying contact areas Ensure that all new tubing stainless steel is passivated and thoroughly flushed before making pump connections Never allow large pressure drops in the system because this can damage the pulse damper membrane To avoid such pressure drops remove fittings of high pressure tubing only after the pump is switched off and the pressure has dropped to atmospheric pres sure When the ALEXYS LC EC system is not used for a longer period of time weeks follow the system shutdown procedure Precautions and system shutdown The ALEXYS system has been developed for continuous operation For maximum stability it is advised to leave the system ON continuously If preferred the flow cell may be switched OFF at night However precau tions should be taken when Chapter 5 Maintenance 33 e system is not in use for longer period of time more than a few days e using mobile phase buffers at pH lt 2 or pH gt 9 e using corrosive mobi
16. ent Front side Fig 10 front side of the OR 100 organiser rack Element Description 1 P Degasser power LED green Indicates when power is applied to the degasser power cord connected and the power switch at the back panel ON 2 S Degasser status LED orange Indicates when the vacuum exceeds the operating range It may also indi cate an error condition The LED will flash in case of an internal leakage or a vacuum signal error 3 V Degasser vacuum LED green Indicates when the vacuum level is within operating range LED will go on 26 User manual OR 100 110 edition 4 Element Description after the initial pump down and will remain on as long as the degasser is powered up and the vacuum level is below 100mm of Hg absolute 0 13 bar 4 Degasser In and outlet port of degasser channel 1 Fitting 4 28 solvent female One port inlet should be connected by ports 1 tubing via the in line mobile phase filter to the mobile phase container other port outlet to the inlet of the pump 5 Degasser In and outlet port of degasser channel 2 Identical to solvent degasser channel 1 ports 2 6 Pulse Stainless steel membrane type pulse damper Fitting damper 1 16 32 female Bottom inlet top outlet 7 Solvent tray Tray for storage of bottles with mobile phase waste and solvent for pump piston back flush 8 Alignment Stainless steel plate t
17. ereby declares that the product ALEXYS OR 100 110 organiser rack type 184 to which this declaration relates is in conformity with the following direc tives Safety 73 23 EEC Safety requirements for laboratory equipment EN61010 1 2001 Class Installation cat Il Pollution degree 2 Immunity 89 336 EEC Electromagnetic immu EN61326 1 1997 A1 1998 nity EN61000 4 2 EN61000 4 3 ENV50204 EN61000 4 4 EN61000 4 5 EN61000 4 6 EN61000 4 8 EN61000 4 11 Emissions 89 336 EEC Electromagnetic emis EN61326 1 1997 A1 1998 sion EN55011 Class B EN61000 3 2 EN61000 3 3 Attention Use manufacturer supplied power and I O cable s only to connect all other devices with the OR 100 110 Manufacturer will not accept any li ability for damage direct or indirect caused by connecting this instrument to devices which do not meet relevant EMC and safety standards September 25 2008 Introduction iii Intended use For research purposes only While clinical applications may be shown this instrument is not tested by the manufacturer to comply with the In Vitro Diagnostics Directive WEEE directive All equipment of Antec Leyden which are subjected to the WEEE directive shipped after August 13 2005 are compliant with the WEEE marking re quirements Such products are labelled with the crossed out wheelie depicted on the left site The symbol on the product indicates that the product must not be
18. f salts may occur Al ways switch off flow cell first Start Up Test and Pump Operation Immediately upon turning on the instrument the microprocessor examines the vacuum sensor signal to confirm that it is within an expected range Following the start up test the microprocessor ramps the vacuum pump to high RPM to quickly exhaust atmosphere from the vacuum chamber As the vacuum level approaches the preset control value the pump will slowly ramp down to a low speed typically 40 to 60 RPM Afterwards the pump RPM will vary slightly as needed under the changing degassing load to maintain a virtually constant vacuum level 50 mm Hg 70 mbar This is necessary due to the extremely low mass high response of the de gassing device hence ensuring a highly stable baseline Smart Leak Detection A potential leak in the vacuum degassing system can be observed by monitoring the RPM of the pump If a leak occurs inside the chamber the microprocessor will increase the pump RPM to maintain the vacuum level If the pump fails to do so if it runs at an elevated RPM for more than 30 minutes the yellow LED will flash indicating a possible leak condition and the system will shut down and go into a safe mode Powering up the degasser With the OR 100 connected to mains with a power cord switch on the degasser by means of the power switch at the rear panel of the OR 100 The green Power LED illuminates Immediately upon turning
19. ions OR 100 110 CAUTION Never push the solvent through the channel 16 User manual OR 100 110 edition 4 Installation of OR 110 The installation procedure of the OR 110 consists of the following steps 7 In case the OR 110 was ordered as a part of the ALEXYS system based on the new LC 110 pump Remove the aluminium instru ment support on the bottom of the unit This can be achieved by removing the two Allen hex screws on the left side panel which fix the aluminium support to the OR 110 housing see figure 2 In case the OR 110 is not used in combination with an ALEXYS sys A tem do not remove the aluminium support 2 Position the system components and OR 110 as displayed in fig ure 7 or 8 with a distance of a few centimetres between the equip ment 3 s O T Fig 7 ALEXYS system with one pump and AS 100 top or AS 110 bottom autosampler CHAPTER 2 Installation guide 17 Fig 8 ALEXYS system with two pumps and AS 100 top or AS 110 bottom autosampler 5 Incase the OR 110 was purchased as a part of an ALEXYS system please refer to the LC connection kit install guide supplied with the ALEXYS system for further instructions If the OR 110 was purchased stand alone so without ALEXYS sytem use orange striped PEEK tubing 1 16 OD 500 um ID to make the LC connections on the high pressure side so from pump outlet gt pulse damper and pulse damper gt injector Use FEP tubing 1 8 OD 1 16 ID
20. iser rack is shipped with a number of parts The listing in the table below may not be complete see checklist of delivery for com plete listing Table VIII Accessories OR 100 organiser rack Part no 184 0204 250 0115 250 0116 250 0140 250 0141 250 0143 250 1550 250 1552 250 1554 250 1556 Description OR 100 mobile phase tray drain Fuses 8AT 250V Mains cable EUR OR 100 110 power cord 0 6 m OR 100 110 power cord 1 8 m OR 100 110 power cord 5m Ferrule for OR 100 110 degasser Nut for OR 100 110 degasser Ferrule for OR 100 110 pulse damper Nut for OR 100 110 pulse damper The OR 110 organiser rack is shipped with a number of parts The listing in the table below may not be complete see checklist of delivery for com plete listing Table IX Accessories OR 110 organiser rack Part no 250 0115 250 0116 250 0140 250 0141 250 0144 250 1550 250 1552 250 1554 250 1556 Description Fuses 8AT 250V Mains cable EUR OR 100 110 power cord 0 6 m OR 100 110 power cord 1 8 m OR 110 LC 110 power cord Ferrule for OR 100 110 degasser Nut for OR 100 110 degasser Ferrule for OR 100 110 pulse damper Nut for OR 100 110 pulse damper Chapter 7 Index Index ACCESSOSIES n nenene 38 cables POWER cceseeseeeeeseeeeees 19 21 se al spa enasna 19 22 degaSSer eis chide 28 degasser LED cere 25 degasser power switch 27 degassing principle 28 GAIN aate
21. le phase Always switch off flow cell when changing to solvents with out electrolytes First read column manufacturer recommendations Take out column in case of solvent incompatibility Start always with flushing LC system with water when switch ing solvents Corrosive solvent precautions When the system is not in use overnight or weekends switch off the cell and read column manufacturer recommendations Flush with an appropri ate solvent and only take out column in case of solvent incompatibility Flush system with pure water and set the HPLC pump to a low flow rate Next working day change water for mobile phase and continue operation Before switching on the cell make sure mobile phase has been flushed throughout the system Corrosive solvents are high concentrations of sodium hydroxide carbo hydrate analysis or other buffers of pH gt 9 or pH lt 2 System shutdown When not in use for a number of days flush the OR 100 110 first with water then with iso propanol or 50 50 water methanol Store column and flow cell according to manufacturer recommendations Then turn off all ALEXYS system components Turn off the OR 100 Disconnect all tubing from the degasser and pulse damper Plug all of the ports on the OR 100 with the appropriate caps Before using the OR 100 110 again completely purge the degasser and pulse damper with the correct mobile phase before reconnecting the col umn and restarting the system Avoid precipi
22. ll guide p n 190 7004B carefully Every individual ALEXYS system component is shipped with a specific RS232C cable for computer control These cables can be found in the corresponding ship kits The part numbers are listed in Table VI 22 User manual OR 100 110 edition 4 Table VI Serial cables of ALEXYS 100 LC EC system components Part no Description Qty 250 0122 DECADE II RS232 cable 181 0596 AS 100 serial cable 9M 9F pins 191 0528 AS 110 serial cable 9M 9F pins 193 0412 LC 110 serial cable 9F 9F pins Depending on the configuration purchased l 2 The serial cables for the detector autosampler and pump specified in Table VI should be connected to PC COM port 7 8 and 9 respectively The serial cable of a second pump should be connected to COM 10 Use always D A P DECADE II Autosampler Pump order of connect ing devices to be compatible with pre configured device drivers in ALEXYS data system Table VII Connection scheme RS232 cables on PC COM ports PC port Part no Connect to COM7 250 0122 DECADE II COM8 181 0596 or 191 0528 AS 100 or AS 110 autosampler COM9 193 0412 LC 110 serial cable 9F 9F pins COM10 193 0412 LC 110 serial cable 9F 9F pins Please refer to the manuals of the individual system components LC 110 AS 100 110 and DECADE II for specific installation instructions These manuals contain important information about the installation maintenance and safety practices Individual
23. nt flowing through the degasser will continue to be de gassed as long as the instrument is on and running Extending the degassing flow rate range Certain organic solvents used in reversed phase chromatography outgas upon mixing with water if not properly degassed These solvents are gen erally alcohols e g methanol acetonitril and tetrahydrofuran Passing water and methanol through a single channel is generally sufficient to de gas these solvents To more thoroughly degas a mobile phase connect the outlet of the or ganic channel to the inlet a second channel and the outlet of the second channel to the pump This places the two channels in series and doubles the degassing capacity for the organic portion of the mobile phase Chapter 4 Operation 31 OR 100 110 integrated pulse damper Principle of operation The OR 100 110 is equipped with a membrane type pulse damper with stainless steel housing Pump pulsations are strongly suppressed improv ing detection limits in HPLC EC The pulse damper reduces pulsations by compressing a fluid held within the bottom cavity of the unit This fluid is isolated from the LC mobile phase by a durable but flexible inert diaphragm As the LC system pres sure increases the fluid compressed When the LC pump piston is retract ing the compressed fluid expands maintaining system pressure and more important keeps the mobile phase flowing at a constant rate When the system pressure is at its maxim
24. o align and fix the LC 100 pump plate and AC 100 controller into the organiser rack The instrument feet of the pump fit in the holes in the align ment plate 9 Does not apply for the OR 110 CHAPTER 3 Description of instrument Back side 27 Fig 11 Back side of the OR 100 organiser rack Description Degasser power switch Element 1 Power switch 2 AC outlets 3 Mains inlet Block of 8 AC outlets to connect all ALEXYS system components such as pump autosampler and DECADE II to mains Mains inlet with in line fuse holder to supply power to the integrated degasser and the 8 AC outlets 4 Drain Drain for tubing connection to prevent possible leak age of solvents from the tray onto the electrical equip ment stacked below does not apply for OR 110 28 User manual OR 100 110 edition 4 CHAPTER 4 Operation OR 100 110 integrated dual channel degasser Principle of Operation The OR 100 110 vacuum degasser has 2 vacuum chambers degassing tube variable speed vacuum pump microprocessor controller sensor and check valves The solvent mobile phase flows through a degassing tube which is inside a vacuum chamber In accordance with Henry s Law de creased pressure in the chamber causes the outward movement of gas dissolved in the mobile phase across the tube wall thus degassing the mobile phase The pressure in the vacuum chamber is established by the vacuum
25. r t a 12 electrical hazards 006008 V environmental conditions 12 fuse replacement 33 fuse replacement 08 v installation kit 13 leak detection c c ccceeeeeee 29 lOakAage iiiiecisselieaie estes 36 LED degasset cceeeeeee 25 lift instruction seenen 13 maintenance 32 pulse damper 31 safety practices 0 eee v specifications OR 100 37 trouble Shooting 35 39
26. rvice membrane ruptured Leakage of mobile phase via OR 100 drain bottom plate Possible cause Remedy Internal leakage of degasser Contact your local supplier for service vacuum chambers or tubing Internal leakage of pulse Contact your local supplier for service damper When trouble shooting is not successful contact supplier Chapter 6 Specifications ALEXYS OR 100 110 Organiser Rack 37 CHAPTER 6 Specifications ALEXYS OR 100 110 Organiser Rack General Power specifications Operating conditions Storage conditions Power outlets Degasser Pulse damper Physical Dimensions specifications 100 240 VAC 50 60 Hz 20 VA degasser only 10 35 C 20 80 RH non condensing 0 50 C 20 80 RH non condensing 8 outlets 100 VAC max 800 VA total 240 VAC max 1900 VA total max 300 VAC per outlet 2 channels volume 480 uL channel ca pacity lt 2 ppm O at 1 mL min max flow rate 10 mL min LED for power start up and vacuum level wetted materials PEEK PTFE Teflon AF membrane type dead volume 0 9 mL at max pressure 41 4 MPa 6 000 PSI wetted materials 316 SS and FEP OR100 35 D x 39 W x 44 H cm 13 6 D x 15 2 W x 17 3 H OR 110 35 D x 9 W x 44 H cm OR 100 12 5 kg 27 5 Ibs with 1 pulse damper OR 110 10 3 kg 22 7 Ibs with 1 pulse damper 38 User manual OR 100 110 edition 4 CHAPTER 7 Accessories The OR 100 organ
27. s are summarized in the Certificate of Performance shipped with the OR 100 110 Operating environment Install the OR 100 110 and ALEXYS LC EC System in an area which meets the environmental conditions listed in Table Place the OR 100 110 on a flat and smooth surface Table I Environmental conditions Parameter Requirement Storage temperature 0 50 C Storage humidity 0 80 non condensing Operating temperature 10 35 C Operating humidity 20 80 non condensing The OR 100 110 has a drain at the bottom plate located at the front side under the pulse damper This drain is to dispose of liquids due to possible internal leakage of the pulse damper or degasser Do not close or block this drain The manufacturer will not accept any liability for damage direct or indirect caused by connecting this instrument to devices that do not meet the rele vant EMC and safety standards The pump connected to the system CHAPTER 2 Installation guide 13 should be specifically designed for use in LC and capable of delivering flow rates typically in the range between 1 L min up to 10 mL min Use of this product outside the scope of this manual may present a hazard Do NOT connect OR 100 110 to mains until the entire installation pro cedure is completed Installation liquid connections This section is NOT a complete installation description for a complete ALEXYS LC EC System It describes the installation of the OR 100 1
28. ship kits of ALEXYS 100 components have a small overlap of parts with ALEXYS installation kit Therefore In case of a complete ALEXYS system installation a few superfluous parts in the kit are left over CHAPTER 2 Installation guide 23 Powering up system Last step in the installation procedure is connecting OR 100 110 to mains and powering up all devices Before connecting power cords check fuse rating and mains power specifications of ALL connected devices See user manuals for de tails OR 100 110 does not have a power switch Plugging in power cord to mains immediately powers up all connected devices 1 Make sure that the power switches of all connected devices are switched OFF 2 Connect mains cable p n 250 0116 to OR 100 110 mains entry see Fig 9 connector 1 Plug in power cord in wall socket to power up system 3 Switch on OR 100 110 degasser LEDs light up indicating system is functioning OR 100 110 is now ready for use Switching off your ALEXYS LC EC system is not recommended The ALEXYS LC EC system has been developed for continuous opera tion For maximum stability it is advised to leave the system ON continu ously If preferred the flow cell may be switched OFF at night If not in use for longer period of time more than a few days see recommendations for system shutdown 24 User manual OR 100 110 edition 4 CHAPTER 3 Description of instrument 25 CHAPTER 3 Description of instrum
29. t the 1 8m power cord p n 250 0141 to the DECADE II de tector and socket 2 of the OR 110 see Fig 9 CHAPTER 2 Installation guide 21 e Connect the 0 6 m power cord p n 250 0140 to the AS 100 or AS 110 autosampler and socket 3 of the OR 110 e Connect the OR 110 LC 110 power cord p n 250 0144 to the LC 110 pump and socket 4 of the OR 110 If the system has 2 pumps connect the second pump to socket 5 A N Do NOT connect OR 110 to mains power at this point yet Table V Connection scheme OR 100 power cables Outlet no Part no Connect to 1 250 0116 Mains 2 250 0141 DECADE II detector 3 250 0140 AS 100 110 autosampler 4 250 0144 LC 110 pump Use the manufacturer supplied power cable s only to connect all other devices with the OR 110 Manufacturer will not accept any li ability for damage direct or indirect caused by connecting this in strument to devices which do not meet relevant safety standards Serial connections The first step to establish all serial connections is to install the Serial port connection kit p n 190 0202B or 190 0204B supplied with your ALEXYS LC EC system This kit consists of one or two 2 port serial PCI cards which needs to be installed in the PC which controls the LC system This kit will add 2 or 4 COM ports to the PC necessary for the serial connec tions with your LC system components For the installation and configura tion of the PC COM ports follow the instructions in the insta
30. tation of buffer salts in organic solvents Start always with flushing system with water when switching solvents Replace blown fuses with fuses of proper type and rating 8AT 250V UL CSA approved stipulated on the side panel and specified in the instal 34 User manual OR 100 110 edition 4 lation section of this manual The fuse holder is integrated in the mains inlet V 100 240V FUSE RATING 50 60Hz E amp E 8AT 250V WARNING RISK OF FIRE REPLACE FUSE AS MARKED AVERTISSEMENT RISQUE DE FEU REMPLACEZ LE FUSIBLE COMME INDIQU Fig 14 Fuse holder To access the fuse holder push plastic lever on the bottom side of the holder completely and pull holder out of inlet Ensure that the instrument is never put in operation with fuses of a different type This can cause fire Chapter 5 Trouble shooting guide CHAPTER 5 Trouble shooting guide 35 All ALEXYS system components are off Possible cause No power Power switch off Faulty fuses Divergent mains voltage Degasser LEDs are off Possible cause No power Power switch off Faulty OR 100 mains fuses Divergent mains voltage Remedy Plug in power cord Turn power switch on Replace fuses Check line voltage see page 11 Remedy Plug in power cord Turn power switch on Replace fuses Check line voltage see page 11 Degasser yellow status LED is on or flashing Possible cause Yellow LED is on Yellow LED is flashing Remedy Typi
31. um allowable pressure 40 MPa 6000 psi the volume of mobile phase in the damper is only 0 9 mL HPLC pump Injector Membrane lso propanol Pulse Damper Fig 13 Schematic representation of OR 100 pulse damper Operation of the pulse damper is automatic The pulse damper works best if the pressure is at least 35 bar 500 psi 3 5 MPa and preferably 70 bar 1000 psi 7 0 MPa In case of low pressure it may be desirable to install a restrictor between damper and injector The advised operating tempera ture is between 15 35 C CAUTION Never induce large and sudden pressure drops in the sys tem because this will damage the internal diaphragm of the pulse damper resulting in leakage To avoid pressure drops remove fittings of high pressure tubing only after the pump is switched off and the pressure has dropped to atmospheric pressure 32 User manual OR 100 110 edition 4 CHAPTER 5 Maintenance Perform preventive maintenance to ensure that your OR 100 110 with integrated degasser and pulse damper will perform consistently at an op timal level The following measures are recommended Use only high purity solvents preferably HP LC Grade for mo bile phases Water should be bottled LC Grade or ultra pure 18 MOhm cm Filter all solvents to prevent particulate contamination and plugged tubing Perform periodic leak checks on LC tubing and connections on the degasser and pulse damper of the OR 100 110 Check if th
32. um power which can be drawn from these power outlets is 1900 Watt at 240VAC 960 Watt at 120V and 800 Watt at 100 VAC The maximum rated power per outlet is 300 Watt Do not exceed the maximum rated power this can cause fire or other po tentially dangerous situations It is advised to use the outlets only to con nect ALEXYS system components such as the LC 100 pump AC 100 controller AS 100 autosampler and DECADE II electrochemical detec tor In case of the OR 100 not applicable for OR 110 always have the silicone tubing connected which is in the ship kit to the drain of the mobile phase tray at the back side of the OR 100 to prevent leakage of solvents onto the electrical equipment below LC 100 pump or AC 100 acquisition con troller Leakage of solvent on the connectors on the back of the electrical equip ment can lead to short circuit Introduction vii General precautions Execute periodic leak checks on LC tubing and connections Do not allow N flammable and or toxic solvents to accumulate Do not close or block drains Follow a regulated approved waste disposal program Never dis pose of such products through the municipal sewage system Use of this product outside the scope of this manual may present a hazard Spare parts and service availability Manufacturer provides operational spare parts of the instrument and cur rent accessories for a period of five years after shipment of the final pro
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