Valve INV-907
Contents
1. Channel plate Distribution plate Valve INV 907 Instructions 56 8101 01 Edition AC Slide the screws out 4 Remove the distribution plate containing the ports 5 Remove the old channel plate and insert a new one 6 Remount a new distribution plate so that the text 3 is horizontal and to the right of the central tubing connection Using the Allen key tighten the 4 screws in turn a little at a time until the distribution plate is fixed to the valve body Valve INV 907 Instructions 56 8101 01 Edition AC 5 Trouble shooting If the suggested actions do not correct the fault call Amersham Pharmacia Biotech Fault Action The valve is not switching 1 Check the connection to the pump The valve should be connected to the UniNet 2 socket not the UniNet 1 socket 2 Check the ID switch on the valve The ID number should correspond to the number set in UNICORN 3 Check the UniNet cable and replace if required The valve is switching to wrong position The valve parts may have been incorrectly assembled after replacement 1 Check that the distribution plate marking 3 is horizontal External leakage 1 Check the tubing connections Tighten or replace if required Internal leakage Internal leakage are easy to detect at the small hole on the underside of the valve body 1 Internal valve parts may be worn Change channel plate and distribution plate according to section 4 High back pressure2. 1 Introduction Valve INV 907 is a motorised rotary 7 port valve The valve is used in AKTA design chromatography systems It is powered from the AKTAdesign P 900 series system pump and is controlled from UNICORN control system The valve has 3 positions used for loading a sample loop injecting the sample onto the column washing the system pump The valve can also be used to switch between upflow and downflow in a column Features e Completely swept flow path minimises eluent or sample memory effect e Flow rates up to 100 ml min e All wetted parts are plastic PEEK Safety WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken 2 Installation CAUTION Before connecting Valve INV 907 ensure the power is switched OFF at the system pump or the complete system Unpacking Unpack the valve and check the items against the packing list Inspect the items for obvious damage which may have occurred during transportation Installing the valve 1 Connect the valve with two UniNet cables as a part of the UniNet 2 chain i 2 Set the ID switch at the Os bottom to UniNet 2 network 56 8101 01 Edition AC Valve INV 907 Instructions the required valve number 0 9 The number should correspond to that used in UNICORN Note All valves must have different
3. Item Quantity Code no per pack Valve INV 907 including one UniNet cable fill port needle and syringe holder are not included 1 18 1108 40 Injection kit INV 907 including fill port needle and syringe holder 18 1110 89 Valve kit INV 907 including channel plate and distribution plate 1 18 1109 05 Sample loops 100 ul 1 18 1113 98 500 ul 1 18 1113 99 1 ml 1 18 1114 01 2 ml 1 18 1114 02 Superloop 10 ml 1 18 1113 81 Superloop 50 ml 1 18 1113 82 Superloop 150 ml 1 18 1023 85 Cable UniNet 0 7 m 1 18 1109 74 Mounting bracket 1 18 1109 11 Teflon tubing i d 1 8 o d 3 16 3m 18 1112 47 Tubing connector for 3 16 o d tubing 10 18 1112 49 Ferrule for 3 16 0 d tubing 10 18 1112 48 Stop plug 5 16 5 18 1112 50 Stop plug 1 16 5 18 1112 52 Union Luer female 1 16 male 2 18 1112 51 Union 1 16 female M6 male 6 18 1112 57 Union M6 female 1 16 male 8 18 1112 58 PEEK tubing i d 0 75 mm o d 1 16 2m 18 1112 53 Teflon tubing i d 0 75 mm o d 1 16 2m 18 1112 54 PEEK tubing i d 1 0 mm o d 1 16 2m 18 1115 83 Fingertight connector 1 16 10 18 1112 55 Valve INV 907 Instructions 56 8101 01 Edition AC Important user information WARNING The Warning sign is used to call attention to the necessity to follow an instruction in detail to avoid personal injury Be sure not to proceed until the instructions are clearly understood and all stated conditions are met CAUTION The Caution sign is
4. clogging 1 Do cleaning in place according to the instructions in section 4 2 Change channel plate and distribution plate according to section 4 Other faults Contact Amersham Pharmacia Biotech Reference information Description The valve consists of two main parts i Housin e Housing which 4 encloses the motor and Valve body electronics e Valve body with a rotating central core As the channel plate is turned by the motor different ports are eal Valve switching is controlled from UNICORN by reading the actual position of the channel plate The geometry of the valve assures that the flow path is completely swept so that solvent or sample memory effect is virtually non existing The valve rotates the shortest way to the next position max 180 The material used in the switching parts ensures both long mechanical and chemical lifetime The valve housing contains no user replaceable items Technical specifications Operating data Max Flow rate Max Pressure 100 ml min 25 MPa 250 bar 3600 psi Back pressure lt 50 kPa at 100 ml min with water Leakage lt 0 1 l min at 25 MPa pH stability range 1 13 1 14 lt 1 day exposure Viscosity Max 5 cP Switch time lt 260 ms between two adjacent positions Operating life time gt 50 000 cycles two adjacent positions 4 to 40 C 20 95 relative humidity 84 106 kPa 840 1060 mbar atmospheric pressure Environment
5. numbers 3 Mount the valve vertically in the way it is shown in the user documentation of your AKTAdesign chromatography system 4 Apply an identification number from the labels supplied The number should be the same as that of the rotary switch at the bottom of the valve Place the number so that it is easily readable from the front Ae 5 Connect the tubing O amersham pharmacia biotech 6 When used as an injection valve connect the tubing as follows Port Connect to 1 Column inlet 2 Sample loop or bottom tubing of Superloop Injection port Waste Waste Sample loop or top tubing of Superloop Outlet of the pump ou w N LOAD position 1 1 Column Pump Sample syringe 7 When used as an upflow downflow valve connect the valve as indicated in the user documentation of your KTAdesign chromatography system 3 Operation The valve is controlled from UNICORN When used as an injection valve use the instruction InjectionValve in System Control Manual Flowpath The valve can be set in 3 positions e LOAD position 1 home position e INJECT position 2 e WASTE position 3 If the valve has not been activated it is in position 1 INJECT position 2 Column WASTE position 3 Column Valve INV 907 Instructions 56 8101 01 Edition AC Manual sample loading The sample loop and Superloop can be loaded with a syringe Connect Superloop with the bottom
6. Physical data Internal volume Pos 1 LOAD Port 1 7 9 ul Port 2 3 5 ul Port 4 6 8 ul Pos 2 INJECT Port 1 2 7 ul Port 3 5 6 ul Port 6 7 9 ul Pos 3 WASH Port 2 4 8 ul Port 5 7 9 ul Flow channel diameter 0 8 mm Valve principle Motor controlled valve Functions Switching 3 positions controlled from UNICORN Degree of protection IP 43 Wetted materials PEEK polyetheretherketone Chemical resistance The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 ethylacetate 100 hexane and 100 tetrahydrofuran THF Power requirement 32 V DC 10 from the system pump Power consumption Up to 9 W UniNet 2 address 0 9 Inlet and outlet tubing UNF 10 32 2B Fingertights for capillary tubing 1 16 outer diameter Dimensions HxWxD 135 x 80 x 120 mm Weight 1 2 kg EMC Standards This product meets the requirement of the EMC Directive 89 336 EEC through the harmonized standards EN 50081 1 emission and EN 50082 1 immunity Note The declaration of conformity is valid for the instrument when it is e used in laboratory locations used in the same state as it was delivered from Amersham Pharmacia Biotech except for alterations described in the user manual connected to other CE labelled Amersham Pharmacia Biotech instruments or other products as recommended Valve INV 907 Instructions 56 8101 01 Edition AC Accessories and spare parts
7. tubing connected to port 2 and the upper tubing to port 6 For large volumes use a luer female 1 16 male union connected to port 3 For smaller volumes use an injection needle together with the injection fill port as described below Check the fill port nozzle is screwed into the fill port Note The thread is left handed 1 Loosely thread the fill port screw into valve port 3 2 Insert the injection needle 0 7 mm o d into the injection fill port 3 Tighten the fill port until the nozzle has formed a seal around the needle tip When the seal is adjusted correctly it feels as if you are penetrating a septum at the end of the injection fill port The seal should provide easy insertion and removal of the needle 4 Mount the syringe holder on the fill port Valve INV 907 Instructions 56 8101 01 Edition AC Two techniques can be used for filling the sample loop partial or complete filling Type of filling Volume to load Partial filling max 50 of the sample loop volume Complete filling 2 5 times the sample loop volume Partial Filling Partial filling is used when high recovery is required Partial filling allows the injected volume to be changed without changing the loop and does not waste sample Partial filling is achieved as follows 1 Set injection valve to position LOAD 2 Load the syringe with a large volume of buffer 5x the loop volume 3 Fill the sample loop ca
8. used to call attention to instructions or conditions that shall be followed to avoid damage to the product or other equipment Be sure not to proceed until the instructions are clearly understood and all stated conditions are met Note The Note sign is used to indicate information important for trouble free or optimal use of the product Should you have any comments on this instruction we will be pleased to receive them at Amersham Pharmacia Biotech SE 751 84 Uppsala Sweden Trademarks AKTA and UNICORN are trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries Amersham is a trademark of Nycomed Amersham plc Pharmacia and Drop Design are trademarks of Pharmacia amp Upjohn Inc Terms and Conditions of Sale All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Pharmacia Biotech group which supplies them A copy of these terms and conditions of sale is available on request Adresses Amersham Pharmacia Biotech UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Pharmacia Biotech AB SE 751 84 Uppsala Sweden Amersham Pharmacia Biotech Inc 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Pharmacia Biotech AB 1998 All rights reserved o amersham pharmacia biotech TC information ab Uppsala Printed in Sweden by TK i Uppsala AB October 1998
9. refully with buffer 4 Set the injection valve to position INJECT before taking out the syringe Note If the syringe is taken out when the injection valve is in position LOAD self drainage will occur and air will enter the sample loop 5 Load the syringe with the required volume of sample No more than half 50 a loop volume of sample should be loaded into the loop 6 Insert the syringe in position 3 on the injection valve Set the injection valve to position LOAD Note Do not load the sample before the valve is in position LOAD 7 Gently load the syringe contents into the loop 8 Leave the syringe in position The sample will be injected onto the column when the valve is switched to INJECT in the method Complete filling In this method a surplus of sample is used to ensure that the sample loop is filled completely allowing reproducible sample volumes In preparative applications the sample volume should be at least 2 times the volume of the sample loop For analytical reproducibility a sample volume 5 times the volume of the sample loop should be used With complete filling the sample volume can only be changed by changing the loop size Complete filling is achieved as follows Set valve to position LOAD 2 Load the syringe with sample 2 5 times the loop volume 3 Gently load the syringe contents into the loop 4 Leave the syringe in position The sample will be injected onto the column when the val
10. ve is switched to INJECT in the method Note If the syringe is taken out before the sample is injected onto the column self drainage will occur and the loop will be emptied Emptying the sample loop When emptying the sample loop a buffer volume of approx 5 times the sample loop volume should be used to flush the loop and ensure that all sample is injected onto the column Storage Overnight The valve can be left filled with a buffer Weekend or Long time storage Flush the valve with water and then fill it with 20 Ethanol 4 Maintenance CAUTION Only spare parts approved or supplied by Amersham Pharmacia Biotech may be used for maintaining and servicing the valve Action Change channel plate and distribution plate Period Every 12 months or when required Cleaning in place Pump a cleaning or sanitizing agent through the valve The standard recommendation is to pump 1 M NaOH for 30 minutes and then wash out with buffer WARNING NaOH is injurious to health Avoid spillage Changing channel plate and distribution plate A replacement kit Valve Kit INV 907 is available see ordering information in Reference Information 1 Ensure that the valve is in position 1 and then disconnect it from the pump 2 Remove the 4 screws on the front using the supplied 3 mm Allen key Loosen each one equally in turn so the distribution plate comes off parallel to the valve body
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