Electrophoresis Power Supply User Manual EPS 3500
Contents
1. 1 The front panel of the EPS 3500 4 1 2 Keyboard Enter Enters a value locks the value if correct and moves programming to the next field Valid values are voltage 35 3500 V current 1 150 mA power 1 100 W time 0 01 500 h volthours 1 500 000 Vh 4 Technical description 01 continue E Set Puts the instrument into SET its programming and starting mode In the RUN mode pressing also shows the programmed parameters for the actual run In addition allows you to make changes in the program during a run after first pressing Change up Change down Changes the parameter value or other choice in the field which is blinking Numerical values are changed in an accelerating manner when a key is held down Clicking a key changes the value in preset increments Parameters or units e g Vh and YES NO choices are changed with one key push The keys can also be used to switch between time and volthours in RUN PAUSE and END The values scroll i e they automatically change from maximum to minimum value or vice versa Run Pressing El starts the run and puts the program into RUN mode The program number current values for voltage current and power are shown on the display The elapsed time or volthours are also displayed Switch between time and volthours with m M Pause Puts the instrument in PAUSE mode and svvitches off the voltage The display shovvs the status of the run at the time the key vva2. The output is switched off and an error message is shown in the display FAIL Code No xxx Call service Please read the error code number and contact your Pharmacia LKB Biotechnology representative 8 Technical specification 8 Technical specifications Regulation Output range Programming range Output resolution Programming resolution Accuracy Line regulation Load regulation Ripple Short term stability Long term stability Start current check Sudden load change check Ground leakage check Output protection Recovery after power failure Ambient operating temperature Ambient operating humidity Ambient operating pressure Mains requirements Power consumption Dimensions WxDxH Weight Maximum voltage current and power with automatic cross over at preset limits Voltage 0 3500 V DC Current 0 150 mA Power 0 100 W Voltage 35 3500 V DC Current 1 150 mA Power 1 100 W Time 00 01 500 h Volthour 1 500 000 Vh Voltage 1 V Current 100 HA 0 14 9 mA 1 mA 15 150 mA Power 1 W Voltage 5 V Current 1 mA Povver 1 VV Time 1 min 00 01 99 59 1 h 100 500 h 1 Vh 1 9999 Vh 100 Vh 10 0 99 9 kVh 1 kVh 100 500 kVh Voltage 1 5 V Current 1 1 mA Power 2 1 W Timer 0 1 1 min 00 01 99 59 h 0 1 10 min 100 500 h Volthour lt 0 2 lt 1 at load change 10 90 of maximum load lt 1 at 3500 V 0 2 10 h after warm up lt 1
3. faulty and incorrect use of the product CopyrightO 1994 Pharmacia Biotech AB All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted in any form by any means without permission in written form from the company Contents 1 Introductionis one n Todo dla O sa 3 2 Safety TALO A Odd ca 4 2 Safe precationis sis 4 22 Ansbiilt safety n 4 3 Unpacking and installation A ada 5 AS Technical descripta 6 41 Frontpanel iiit dta land ad 6 A UT Display ba ada ai a 6 412 KeyDOatd tia 6 4 1 3 Output sockets coectetuer ird 8 AZ Reat panela edente ia 8 5 ENOL ATION 9 EL COPIE 9 9 2 OSA ro aider tees en oe 9 Running a ProStreet pe dpi da 11 24 Optional programming ASL 13 do Choosing run parameters coria 14 AAN 16 G EAMES A a ted 17 7 Trouble shooting oodd thes 18 8 Technical specifications unidad 19 9 Ordene information inse teous oap f rer yer 20 1 Introduction 1 Introduction The Pharmacia Electrophoresis Power Supply EPS 3500 is a high quality high precision and safe power supply for electrophoresis applications that require a maximum of 3500 volts EPS 3500 is primarily designed for the following techniques e DNA sequencing e EF Isoelectric focusing EPS 3500 is also suitable for SDS PAGE Polyacrylamide Gel E
4. loading and or changing parameter values by pressing bell Voltage and current will no longer be supplied the HV on LED goes off and vou mav safelv load vour samples The displav shows the status of the run when was pressed Switch between time and integrated voltage bv pressing 2 When sample loading is complete press again or e and the run will continue from where it was interrupted When in the PAUSE mode you can also press to make changes in the program This mode is called the P SET mode When the P SET mode is 12 5 Operation 5 4 Optional programming A entered you can make changes as described under programming The P SET mode is the same as the SET mode apart from restrictions in setting the breakpoint Naturally it is not possible to enter a time or volthour that is already passed Press or to go back from P SET to PAUSE Press ES or to proceed with the electrophoresis View programmed values It is also possible to view the programmed values during a run by pressing Note that no values can be changed here The display returns automatically to show RUN values after 5s Alternatively use or EJ Stopping the run and viewing end parameter values When the programmed time or volthour is attained the program will enter the END mode Itis also possible to break the run manuallv bv pressing bel In both cases the voltage current and povver vvill go to zero as indicated by the HV on LED svvitching off Th
5. 5 Keep the instrument as dry and clean as possible Wipe regularly with a soft damp cloth Let the power supply dry completely before use If wetted unplug the power supply until the instrument is dry 6 Use only undamaged electrical wire and equipment specified for the voltages you will use High voltage wires must meet the requirements of the IEC 1010 2 031 1993 electrical standard Any electrophoresis equipment connected to the power supply should meet the requirements of the IEC 1010 1 1993 electrical standard 7 Note that the output is connected to the chassis reference earth The EPS 3500 is designed in accordance with the IEC 1010 electrical safety standard The power supply also has several built in safety functions Error messages are also shown on the display 1 Functional earth leakage Should the power supply be connected to an electrophoresis unit that has a leakage path to earth the EPS 3500 will detect this fault and the high voltage is turned off 2 Start current check To ensure that an electrophoresis unit is connected correctly the power supply checks that the resistance is not higher than a specified limit at a low safety voltage lt 40V If this resistance is too high the voltage is turned off Too high a resistance can also be caused by using buffers with extremely low conductivity The high voltage is also turned off in this case and an error message is shown This function can be disabled to perform ce
6. e end parameter values are displayed Switch between elapsed time and integrated voltage by An alarm will sound if selected in the program You can stop the alarm by STOP pressing A run cannot be continued after pressing 7 Disconnect the leads and proceed with post electrophoretic techniques Since diffusion will begin as soon as the voltage is turned off you should remove the gel and begin staining blotting or autoradiography immediately You can disable the start current check that otherwise checks that the resistance is not higher than a specified limit This disabling function is set in the MORE mode instead of the SET mode in order not to confuse users who do not need this feature and for safety reasons The MORE mode is described in Fig 5 After pressing the program e asks you for the program number and if you want to check Choose program number and change betvveen YES and NO by mi Iu and confirm with Choosing NO for start current check means that the function is disabled and high voltage can be applied despite a ep the start current very high resistance Leave the MORE mode by Warning disabling the start current check the power supply can deliverhigh voltage even if it is not connected to electrophoresis equipment 13 5 Operation 5 5 Choosing run 14 parameters P24 50 mA 3000 Vh 30 W SET 2500 V 1 Enter MORE Selec
7. ed in section 5 2 Running a program is described schematically in Fig 4 Running Press 7 to start the electrophoresis The display vvill shovv current values for voltage current povver and elapsed time or volthours You can svvitch betvveen shovving the elapsed time or volthours by Hi The limiting parameter will attain the programmed limit The unit for that parameter is underlined A light emitting diode shows when voltage is applied HV on 11 5 Operation 1 Choose Program number SET P1 OFF 3000V 50mA 20VV Select by SET P2 3000 Vh 2500V 50mA 30W 2 Start the run Start by P2A 6 mA RUN 500 V 1Vh 30 W PAUSE P2 500Vh 1200V 25mA 30W SET P2 3000 Vh 2500V 50mA 30W RUN P2 amp 500Vh 1200V 25mA 30W ass 3 During a run you can vievv the settings t is also possible to interupt the run and make changes in the program P SET P24 3000 Vh 2500V 50mA 30W Programming 4 Stop the run manually END P2A 2500 V 50mA RUN P24 2500Vh 2500V 10mA 25W 2500 Vh 25W 5 The run is stopped automaticallv END P2A 2500V 9mA 3000 Vh 23W Fig 4 Step by step summary of run and pause function If no current is displayed please check the electrical connections to the electrophoresis equipment Pausing You can interrupt the electrophoresis for sample
8. esis equipment and controlling by power is recom mended The main part of the IEF is thus controlled by power phase II The conductivity is gradually decreasing because the carrier ampholytes and sample will loose their net charge during the build up of the pH gradient Thus the current will decrease and the voltage increase at constant power During the early stage of the formation of the pH gradient it is important to limit the current Otherwise the gradient will be irregularly shaped phase I The conductivity is not constant along the gel so it is important to also limit the voltage This means running at constant voltage for the last phase phase III to prevent local overheating For detailed information about parameter values always follow the gel supplier s recommendations 15 5 Operation 5 6 Short 16 instructions Preset time Alarm Fig 6 Power fluctuations which occur during a typical IEF run This section summarizes the main points covered earlier in this chapter Use it as a check list once you are familiar with the detailed programming and running instructions Refer also to the separate schematic operating guide included with the power supply We recommend you keep this schematic guide close by the instrument 1 Turn mains power ON The display should have the program number flashing a Press E for the actual program number or use 4 Hi to select the program number desired Pr
9. ess Fi For each of the parameters Voltage Current and Power press ui Hi until the value desired is reached Confirm by pressing E after each one and the display will automatically move to the next parameter Choose between manual break or automatic break For automatic break choose breakpoint units in either Volthours Vh or time h Select the correct unit or for manual break choose OFF with Confirm with If volthours or time were chosen set the value to be used for the breakpoint with 12 If an automatic breakpoint was chosen under 4 you will be asked if you wish an alarm to sound at the end of the run Select YES or NO with Iu Hi and press If YES was chosen this is indicated by a small A as in 1 to the right of the program number in the display 5 Operation 6 Maintenance 7 When programming is completed connect your electrophoresis unit to the outputs and press 8 The actual voltage current and power are shown on the display together with the elapsed time or volthours switch with Iu Hi 9 can be used to interrupt and then continue the run 10 The programmed parameters can be checked during the run by pressing 11 The programmed run stops automatically or by pressing bd f 6 Maintenance Check regularly that the fan is working properly Wipe the instrument regularly with a damp cloth Let the instrument dry completely before use Otherwise no user mainte
10. lectrophoresis e Native PAGE Agarose electrophoresis e Electroblotting DNA pulsed field electrophoresis Electrophoresis separations can be controlled by voltage current or power The EPS 3500 automatically switches over the controlling parameter according to programmed limits and conductivity variations in the system Two electrophoresis units can be connected to the EPS 3500 and run with the same programmed method at one time Three programs can be saved 2 Safety information 2 1 Safety precautions A A 2 2 In built safety features 2 Safety information Extreme caution should be exercised in the operation of this instrument as it can develop sufficient voltage and current to produce a lethal shock To avoid any risk of injury the instrument should only be operated by properly trained personnel and always in accordance with the instructions provided Read this entire manual before using this power supply 1 This instrument is designed for indoor use only 2 The instrument must always be used with the protective earth lead of the power cord correctly grounded to earth at the mains outlet 3 To permit sufficient cooling ensure that the vents in the rear and sides of the instrument are not covered 4 Do not operate the instrument in extreme humidity above 95 Avoid condensation by letting the unit equilibrate to ambient temperature when taking the power supply from a colder to a warmer environment
11. n be followed step by step in Fig 3 Setting voltage current and power limits The display will now flash for the set voltage limit Using the Hi keys select the maximum voltage desired for the run Confirm with Repeat the same procedure for limiting current and limiting power Programmable values for voltage are 35 3500 current 1 150 mA power 1 100 W time 0 01 500 h volthours 1 500 000 Vh Setting break point Choose between manual break or automatic break For automatic break choose breakpoint units in either Volthours Vh or time h Select the correct unit or for manual break choose OFF with 5 Operation 1 Choose Program number 2 Set Voltage limit 3 Set Current limit 4 Set Power limit 5 Choose Breakpoint unit h or Vh or no Breakpoint OFF 6 Set Breakpoint 7 Choose Alarm YES or NO 8 Program ready SET P1 OFF 3000 V 50 mA 20 W Select by mi Confirm by SET P2 OFF 35V 150 mA 100W Select by 6 0 Confirm by SET P2 OFF 2500 V 150 mA 100 W Select by ES Confirm by SET P2 OFF 2500 V 50 mA 100 W Select by zi 4 Confirm by SET P2 OFF 2500 V 50 mA 30 W Select by zi 4 Confirm by SET P2 0 Vh 2500V 50 mA 30W Select by 6 0 Confirm by Alarm at End Y Select by Hi Confirm by SET P2A 3000 Vh SET 2500V 50mA 30 VV 2500 V Breakpoint off P2 50 mA OFF 30 W 10 Fig 3 Step by step summary of pr
12. nance is necessary All servicing should be entrusted to qualified personel only Please contact your local Pharmacia representative for more service information 17 7 Trouble shooting Error message HALT Low start current HALT Ground leakage current HALT Mains Voltage too low HALT Mains fail Program stopped 18 7 Trouble shooting If an error that can be corrected by the user occurs either during a run or when switching on the power supply the program enters the HALT mode and the output is switched off Four different types of errors can cause HALT The following list shows the error message on the display the cause and the remedy Cause The current is less than the lower limit This can be due to incorrect connection of the electrophoresis equipment or due to use of buffers with extremely low conductivity The current to ground leakage in the electrophoresis unit is too high The mains voltage is too low see Specifications Mains power failure for more than 7 s Remedy 1 Check connections and or buffers 2 Press Switch off the start current check in the MORE mode IMPORTANT Read Section 5 4 first A Check the electrophoresis unit Check voltage selector Check mains voltage pause A Press to continue continue H STOP running a program or e le interrupt the run If a serious error occurs the program enters the FAIL mode
13. ogramming 5 Operation 5 3 Running a program Confirm with kal If volthours or h time were chosen the displav will flash for the break value Set the value with Hi Iu and confirm with Choosing alarm When a breakpoint has been entered the following question is shown Alarm at End Y Select YES or NO with m Iu and confirm with isi H YES is selected a small A appears on the right of the program number i e 24 Back to start position The program is novv back to the start position in the SET mode vvith the program number flashing It is possible to go back to this position at any stage during programming in SET mode by pressing or Note that the program is saved automatically vvhen is pressed Disabling the current check See Section 5 4 if you want to use this feature Connect the leads from the electrophoresis unit red to red and black or blue to blue Red is positive and black or blue negative Up to two electrophoresis units can run at the same voltage at one time Please remember to double the maximum current and povver conditions if tvvo units are to be run Voltage will be the same regardless of how many units are run The current should also be doubled if two gels are run on the same unit Choosing a program Press and select the program you wish to run by pressing Hi W until the value after the P is correct Confirm with E Omit this step if you have just programmed a method as describ
14. ooling of electrophoresis equipment is recommended 5 Operation Cooling will also reduce smiling effects which are caused by mobility differentials across an electrophoresis gel resulting from poor heat transfer Since the cooling efficiency cannot be increased indefinitely the power should be limited when programming the power supply The parameter that should be chosen as the constant and thus control the electrophoresis depends on the type of electrophoresis In the case of homogeneous buffers throughout the system same electrode and gel buffer the conductivity is constant during the electrophoresis If the conductivity is constant the voltage will be proportional to the current and the power to the square of the current according to 1 and 2 This means that the result of the electrophoresis will be the same regardless which parameter is chosen for constant For historical and practical reasons voltage is most commonly used for regulation Submarine gel electrophoresis of DNA RNA and pulsed field electrophoresis are usually run at constant voltage SDS PAGE using continuous buffer systems is run at constant voltage or current For discontinuous buffer systems the resistance will increase as the electrophoresis proceeds due to a decrease in conductivity Running at constant voltage will result in decreasing current and power Constant voltage will thus be safe in the respect that the power will not increase and prod
15. p H A R M A C I A B I O T E C H Electrophoresis Power Supply EPS 3500 A izm User Manual 19 3500 05 e Pharmacia Edition AB Biotech Important user information Reading this entire manual is necessary for full understanding and safe use of this product The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument The lightning symbol within an equilateral triangle is intended to alert the user to the risk of exposure to high voltages Should you have any comments on this manual we will be pleased to receive them at Pharmacia Biotech AB S 751 82 Uppsala Sweden Pharmacia Biotech reserves the right to make changes in the specifications without prior notice Warranty and Liability Pharmacia Biotech AB guarantees that the product delivered has been thoroughly tested to ensure that it meets its published specifications The warranty included in the conditions of delivery is valid only if the product has been installed and used according to the instructions supplied by Pharmacia Biotech AB Pharmacia Biotech AB shall in no event be liable for incidental or consequential damages including without limitation lost profits loss of income loss of business opportunities loss of use and other related exposures however caused arising from the
16. rtain applications see 5 4 Optional programming 3 Sudden load change detection This function prevents accidents under running conditions due to a break in the electrical circuit such as a bad connection to the electrophoresis unit The high voltage is turned off in such an event 3 Unpacking and installation A A 3 Unpacking and installation Unpacking Check the contents against the packing list supplied Inspect for any damage that may have occurred during transit Report any damage immediately to your local Pharmacia representative and to the transport company concerned Mains connection Select the appropriate voltage range 100 120 or 220 240 V see Fig 2 Warning If the power supply is connected to 220 240 V with the range set to 100 120 V the instrument can be severely damaged Select the appropriate mains cable and connect one end to the mains socket on the EPS 3500 power supply see Fig 2 and the other end to an AC grounded outlet Local regulation for Great Britain WARNING IMPORTANT This appliance must be earthed The wires in the mains lead are coloured in accordance with the following code Green and yellow Earth Blue Neutral Brown Live If the plug provided is unsuitable for socket outlets the plug must be cut off and suitable plug fitted The cut off plug should be disposed of and must not be inserted into any 13 amp socket as this can result in electric shock The plug or adapter or the distrib
17. s pressed only operates in RUN mode Time and integration values are retained In the pause mode be used to make changes in the program Return to RUN mode by pressing or by pressing Stop Stops the run and puts the instrument in END mode The voltage is switched off and the end parameters are displayed Switch between time and integrated voltage by pressing Hi IDI A run cannot be continued after STOP pressing Continue by pressing to run the same method again or pressing to choose another program program a new method or make changes in an existing method 4 Technical description 4 2 Rear panel More You can disable the start current check that otherwise detects if the resistance is too high and switches off the power See Section 5 4 Exit Stops the execution of an operation such as the entry of a value Only values units that have already been confirmed by 7 Jre retained Returns the instrument to the mode that was left or the start position in SET 4 1 3 Output sockets There are two sets of output sockets to allow two electrophoresis units to be connected and run at the same time see Fig 1 The voltage output is 0 3500 V The negative output socket gives between 0 and 1750 V and the positive gives between 0 and 41750 V The rear panel is shown in Fig 2 On the rear panel there is 1 A mains switch Press in 1 to switch on the power to the power supply Press 0 to s
18. t MORE by MORE Select program Select by 2 Select program Confirm by 3 Choose current check no Current check at start YES no Select by Confirm and Exit by B Back to SET mod ack to mode P2A 50 mA 3000 Vh 30 W SET 2500 V Fig 5 Disabling the current check EPS 3500 is an automatic cross over povver suppiy that allovvs the user to set limits for voltage current and povver During electrophoresis only one of the parameters is limiting at a time The limiting parameter determines together vvith the conductivity in the electrophoresis system the values for the other tvvo parameters Voltage current povver and conductivity are related by the follovving equations U I L 1 P UxI 2 Where U Voltage I Current P Power and L Conductivity Equation 1 is more familiar if the conductivity is replaced by the reciprocal resistance R U RxI Ohm s law The electric field E measured in V cm is the driving force behind electrophoresis E U d where E Electrical field strength U Voltage d distance The electrical field strength is achieved by applying a voltage The higher the voltage the faster the electrophoresis Fast electrophoresis is beneficial since it counteracts diffusion The disadvantage of increasing the voltage too much is that most of the generated electrical energy the product of power and time is transformed to heat Therefore c
19. uce more and more heat On the other hand the separation will slow down and impair resolution due to an increased time available for diffusion Running at constant power would give a faster electrophoresis and controlled power while running at constant current would at the first sight seem to be problematic because of increasing voltage and power During discontinuous electrophoresis however the voltage is not distributed evenly across the gel These gels have a region with low ionic strength that causes a high electrical field strength This region increases as the electrophoresis proceeds This means that the main part of the voltage is spread over a longer and longer distance and a higher and higher power is tolerated This is the reason why constant current is chosen for such applications It is however recommended to also limit the power as a precaution against overheating the gel The power supply will probably switch over to limiting power at the end of the run due to increased voltage The crossing over between different parameters controlling the electrophoresis can be illustrated by IEF isoelectric focusing using carrier ampholytes A graphical representation of the changes in power voltage and current that may occur during a typical IEF run is given in Fig 6 Since the pK values of the carrier ampholytes and the proteins are temperature dependent IEF must be carried out at a constant temperature Therefore cooling of electrophor
20. un simultaneously with the same program Please remember to double the limiting current and power if two electrophoresis units are run at the same time The voltage will be the same regardless of the number of units 4 Technical description 4 1 Front panel 4 Technical description The front panel consists of an alphanumeric display a keyboard with 9 membrane keys a light emitting diode LED that lights when voltage is applied HV on and connectors for two electrophoresis units 4 1 1 Display A 32 digit alphanumeric display guides you through the programming shows current parameter values during the electrophoresis and final parameter values afterwards It also asks questions and shows error messages The display has an upper and lower row Fig 1 shows the display in the start position when power is switched on for the first time The mode in this case SET is shown in the upper row on the left The program number is in the center of the upper row The number shown is that of the previously entered program As no program has yet been entered the first available program number P1 is shown The blinking figure in this case 1 indicates that it can be changed by using the 2 keys The upper right shows the programmed breakpoint In this case itis off The lower left middle and right positions show voltage current and power respectively Electrophoresis Power Supply EPS 3500 Pharmacia Fig
21. ution panel should be provided with 13 amps fuse As the colours of the wires in the mains lead of this appliance may not correspond with coloured markings identifying the terminals in your plug proceed as follows The green and yellow wire must be connected to the terminal in the plug which is marked with the letter E or by the earth symbol or coloured green or green and yellow The blue wire must be connected to the terminal which is marked with the letter N or coloured black The brown wire must be connected to the terminal which is marked with the letter L or coloured red Note After replacing or changing a fuse the fuse cover in the plug must be replaced with a fuse cover which corresponds to the colour of the insert in the base of the plug or the word that is embossed on the base of the plug and the appliance must not be used without a fuse cover Only 13 Amps fuse approved to B S 1362 A S T A should be used Switch on the power Each time the instrument is turned on a self diagnostic test is done If an error is detected during the test a message will appear on the display and an alarm will sound Connection of the electrophoresis unit s Connect the leads from the electrophoresis unit red to red and black or blue to blue see Fig 1 The red lead is the positive and black or blue is the negative Warning Use only undamaged electrical wire and equipment approved for the voltage you will use Two electrophoresis units can be r
22. witch off the power 2 A socket for the mains cable 3 A switch for voltage range The left position corresponds to 100 120 V and the right to 220 240 V 4 Fan vents 100 120 220 240 V AC 50 60 Hz Power 140W 2 5 A Max MAINS Disconnect before servicing Fig 2 The rear panel of the EPS 3500 5 Operation 5 1 Overvew 5 2 Programming 5 Operation The operation of the EPS 3500 is described in the following sequence 1 Programming or editing a method 2 Running a method When the method is running it can be interrupted put in PAUSE mode to apply samples and or change the program During a run it is also possible to look at the program settings Programming and running are discussed in more detail in the follovving tvvo sections Blinking characters are shovvn as bold characters Start position When the power supply is switched on the display shows the start position in SET mode The previous program set is shown and that program number is blinking for example SET P1 OFF 3000 V 50mA 20 W If the power supply is switched on for the first time the breakpoint para meter and the alarm are off and the values for voltage current and power are 35 V 150 mA and 100 W respectively Choosing a program Up to 3 programs can be entered Press to confirm the program shown on the display or use keys to choose another Confirm with The programming procedure ca
23. year Resistance not greater than 1 MO at 40 V current less than 40 Can be disabled Leakage not greater than 500 HA Fully protected against any overload conditions Duration lt 8 s The program continues automatically Duration gt 8s The program continues after manual restart 4 40 C 0 95 106 84 kPa corresponds to 0 2000 m 100 120 V 220 240 V 50 60 Hz Max 140 W 250 x 385 x 95 mm 4 7 kg 19 9 Ordering information 20 9 Ordering information Designation Code No Electrophoresis Power Supply EPS 3500 19 3500 00 v66L 3sn ny eyesddn YL av 4 Aq uspems u paud Pharmacia Biotech
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