ÄKTA™ start - GE Healthcare Life Sciences
Contents
1. List of hazardous substances and their concentrations Indication for each major part if substance exceeds limit Meaning Indicates that this toxic or hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in SJ T11363 2006 SJ T11363 2006 KRAT Indicates that this toxic or hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in SJ T11363 2006 e Data listed in the table represents best information available at the time of publication SJ T11363 2006 36 AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 5 Declaration of Hazardous Substances DoHS List of hazardous substances Component Hazardous substance name Pb Hg Cd Cr6 PBB PBDE Eo R BRERA ZR AKTA start 29 0220 94 1 Frac30 2 11 UNICORN start 29 0187 5112. 5 Safety instructions 3 1 Safety precautions 25 WARNING To avoid damage to person when installing or moving AKTA start follow the instructions below Moving the instrument horizontally One person is recommend ed when moving the instrument horizontally Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before connecting the power cord Power cord Only use grounded power cords delivered or ap proved by GE Healthcare Life Sciences Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect Installing the computer optional The computer should be installed and used according to the instructions provided by the manufacturer of the computer Disconnect power Always switch off power to AKTA start be fore an instrument module is removed or installed or a cable is connected or disconnected CAUTION To avoid damage to person when installing or moving AKTA start follow the instructions below Protective ground AKTA start must always be connected to a grounded power outlet Installing and moving the instrument AKTA start Product Documentation 29 0270 51 AD NOTICE To avoid damage to AKTA start or other equipment when installing or moving the instrumen
3. 1 The product has not been tested as per the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Product AKTA start Product Documentation 29 0270 51 AD 37 4 EC Declaration of Conformity 4 EC Declaration of Conformity GE Healthcare EC Declaration of Conformity We GE Healthcare Bio Sciences AB Declare under our sole responsibility that the product Type of equipment Chromatographic System Brand name AKTA start and Frac30 Code Article no 29022094 29023051 Is in conformity with the provision of the 2006 42 EC Machinery Directive MD And in conformity with the provision of the following EC Directives 2004 108 EC Electromagnetic Compatibility Directive EMC And that the standards referenced have been applied EN 12100 2010 Safety of machinery General principles for design Risk assessment and risk reduction EN 61010 1 2010 Safety requirements for electrical equipment for measurement control and laboratory use EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements This product was CE marked in 2013 Uppsala 10 Sep 2013 Ge e Ewa Bylund Director Regulatory Affairs Declaration No 29064056 GE Healthcare Bio Sciences AB tel 46 18 612 00 00 Bj rkgatan 0 46 18 0 Declaration Part No 29065506 ES us SE 751 84 Uppsala Edition AB Sweden Web site
4. Function AKTA start Product Documentation 29 0270 51 AD 2 General specifications 2 4 Technical specifications 2 4 4 Injection valve Parameter Data 2 4 5 Gradient formation Parameter Gradient flow rate range 0 5 to 5 ml min Gradient composition 596 accuracy Conditions 596 to 9596 B 1 to 5 ml min 0 8 to 2 cP and fresh pump tubing 2 4 6 Pressure sensor Parameter Placement of sensor Pressure sensor is located after the Pump Range 0 to 0 5 MPa 5 Bar Accuracy 0 05 MPa 2 4 7 UV Parameter Wavelength range 280 nm 3 nm Single wavelength Absorbance range 0 1 to 2 AU Linearity Within 5 up to 1 5 AU Operating pressure 0 to 0 5 MPa Flow cell 2 mm optical path length Total cell volume is 30 ul 2 4 8 Conductivity Parameter Conductivity range 0 mS cm to 300 mS cm Resolution 1 mS cm Accuracy 5 or 2 mS cm whichever is greater 20 AKTA start Product Documentation 29 0270 51 AD 2 General specifications 2 4 Technical specifications 2 4 8 Conductivity Parameter Operating pressure 0 to 0 5 MPa Flow cell volume 22 ul Temperature monitor 4 C to 35 C range Temperature monitor 10 or 5 C whichever is greater accuracy 2 4 9 Frac30 Parameter Number of fractions Up to 30 Vessel type e Centrifuge tubes 10 to 12 ml e Falcon tubes 15 ml e Eppendorf tubes 1 5 ml or 2 ml e b5m
5. 1722536 d regun E 50 60 Hz Mfg Yeor 2013 95 V FSALZSQV Protection Class IP21 60 x 569 CEB ma I y nam J t althcore Bio Sciences AB in Sweden y scm Sweden 9 222 Me 6100 1 ANSI UL Sad 61010 La rupture du sceau annule la garantie Warranty void if seal is broken AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 2 Labels Description Placement Keep the pump cover open when not using the system Open the pump cover after you switch off the instrument This product does not comply with the ATEX directive and must not be used in explosive atmo spheres Pinch hazard Switch off the Pump before tubing loading Label on Frac30 The illustration below shows the labels that are attached to Frac30 Description Placement 29025051 Frac30 Instrument label including oan 29002975 Voltage 24 V f b d Mfg Yeor 2013 Max Power 10VA SQ ety sym 0 5 an Specl Protection Closs ip21 9 6 fication A LI GE Healthcore Bio Sc 751 88 Uppsala Swed e Do not open any covers onthe instrument This will void the warranty La rupture du sceau annule la garantie Warranty void if seal is broken 30 AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 2 Labels Safety symbols The following safety symbols are used in the labels Medning Warning Do not use AKTA start before rea
6. Emergency procedures Introduction This section describes how to do an emergency shutdown of AKTA start The section also describes the result in the event of power failure Emergency shutdown In an emergency situation Switch off power to the instrument by pressing the power switch to the O position or by disconnecting the power cord from the instrument The run is interrupted immediately 32 KTA start Product Documentation 29 0270 51 AD 5 Safety instructions 3 3 Emergency procedures WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect Power failure The result of a power failure depends on which unit is affected Power failure to will result in AKTA start The run is interrupted immediately 7 The data collected up to the time of the power fail ure is available on the USB memory stick or if the system is connected to a computer UNICORN start UNICORN start on a The computer with UNICORN start installed shuts computer down On the AKTA start Instrument Display all four touch buttons will be highlighted The run is interrupted immediately Data generated up to 10 seconds before the power failure can be recovered Note The UNICORN start client may close down during a temporary overload of the processor This may ap
7. Page 1 1 Reg No SE 55 61 08 1919 01 http www gehealthcare com Form 70999034 AF 38 AKTA start Product Documentation 29 0270 51 AD Page intentionally left blank GE and GE monogram are trademarks of General Electric Company AKTA and UNICORN are trademarks of General Electric Company or one of its subsidiaries Eppendorf is a trademark of Eppendorf AG Falcon is a trademark of Becton Dickinson and Company All other third party trademarks are the property of their respective owner Any use of UNICORN start is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this Standard Software End User License Agreement is available on request UNICORN start O 2013 2014 General Electric Company 6 2013 2014 General Electric Company All rights reserved First published Sep 2013 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1
8. assembly dispensing the liquid into fractions Tubing holder Holds the tubing used for dispensing the liquid fractions into the collection tubes Collection tubes 10 to 18 mm diameter tubes used to collect the fractions Bowl assembly Holder for collection tubes which supports tubes of four sizes Base unit Case for electromechanical assembly and holder for the Bowl assembly Power on indicator 12 AKTA start Product Documentation 29 0270 51 AD 1 Introduction 1 5 Instrument view Illustration of the instrument modules The illustration below shows the locations and gives brief descriptions of the modules placed on the wet side of the instrument 9 10 Description A3 port valve that is used as a switching valve for gradient formation It enables the use of two buffers which are required for forming a gradient during runs A static mixer that is used for mixing of buffers A and B A 3 port valve that allows either the buffer or the sample to enter the flow path The Sample valve enables direct application of the sample onto the column using the Pump 13 Function 1 Buffer valve 2 Mixer 3 Sample valve AKTA start Product Documentation 29 0270 51 AD Description A peristaltic pump which delivers buffer or sample tothe flow path with a flow rate of up to 5 ml min For cleaning procedures the Pump can flush the flow path at a flow rate of 10 ml min The Pres
9. cm i d 0 75 mm 10 32 UNF connections PEEK tubing Length 23 cm i d 0 75 mm 10 32 UNF connections Marprene tubing Length 25 cm i d 0 8 mm 10 32 UNF connections PEEK tubing Length 13 cm i d 0 75 mm 10 32 UNF connections PEEK tubing Length 17 cm i d 0 75 mm 10 32 UNF connections PEEK tubing Length 15 cm i d 0 75 mm 10 32 UNF connections 17 Parameter System configuration Control system Connection between PC and instrument Dimensions W x D x H Weight excluding packaging Power supply Power consumption Fuse Enclosure protective class Tubing and connectors Inlet Buffer valve to Mixer Mixer to Sample valve Sample valve to Pressure sensor via Pump Pressure sensor to Wash valve Wash valve to Injection valve Injection valve to Column KTA start Product Documentation 29 0270 51 AD 2 General specifications 2 1 System specifications Parameter Doj Ko Column to UV PEEK tubing Length 15 cm i d 0 75 mm 10 32 UNF connections UV to Conductivity PEEK tubing Length 20 cm i d 0 75 mm 10 32 UNF connections Conductivity to Outlet valve PEEK tubing Length 19 cm i d 0 50 mm 10 32 UNF connections Outlet valve to Frac30 PEEK tubing Length 50 cm i d 0 75 mm 10 52 UNF connections Waste tubing ETFE tubing Length 60 cm i d 1 0 mm Fingertight connector 1 16 Sample tubing ETFE tubing Length 25 cm i d 1 0 m
10. AKTA start Product Documentation Page intentionally left blank Table of Contents Table of Contents Emergency procedures Recycling procedures sees Declaration of Hazardous Substances DoHS AKTA start Product Documentation 29 0270 51 AD Introduction cco RR NT 11 Important user information 1 2 Associated documentation ee 1 5 Instrument VIEW ee 14 Process and instrumentation 065 General specifications 2 1 System specifications 2 0 Environmental ranges ee 2 9 Operating Tonge essetis terere band DIRE 244 iii 2 4 1 RU 2 4 2 MINE aie terror eerie CLIE I E 2 4 3 Valves Buffer Sample Wash and Outlet 2 4 4 IE 2 4 5 Gradient formation ee 2 4 6 Pressure SCNSOM Sn 2 4 7 p eet 2 4 8 CONGU Rd 2 4 9 Safety instructions Safety precautions ascribi estote rtectbeedn 1 3 1 5 2 3 3 5 4 EC Declaration of Conformity 1 Page intentionally left blank 1 Introduction 1 Introduction Purpose of this document This document gives an overview of AKTA start general specifications and EC Declaration of Conformity In addition important user information and safety instructions are included For more information about AKTA start and operating instructions refer to the user documentation listed in Section 1 2 Associated documentation on page 7 1 1 Important user i
11. D 1 Introduction 1 2 Associated documentation Access documentation from mobile units Scan the code using your mobile phone or tablet computer to access the product page for AKTA start Select documents to download under the Related Documents tab AKTA start Product Documentation 29 0270 51 AD 9 1 Introduction 1 5 Instrument view 1 5 Instrument view Illustration of the instrument The illustration below shows the main parts of the instrument um 10 AKTA start Product Documentation 29 0270 51 AD 1 Introduction 1 5 Instrument view Description Function Instrument Display User interface for controlling the system and visualization of the runtime data Wet side The modules interconnected by tubing have the following functions e to deliver the liquid in a specified flow path and divert the flow as required to monitor the UV absorbance and conductivity of the liquid Buffer tray Location intended for the placement of buffer bottles used during chromatography runs Power switch Connects or disconnects the power USB port To connect a USB memory stick for storage of results and transfer of files AKTA start Product Documentation 29 0270 51 AD 11 1 Introduction 1 5 Instrument view Illustration of the Fraction collector The illustration below shows the Fraction collector Frac30 Description Function Dispenser arm Holds and positions the tubing holder for
12. Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29 0270 51 AD 06 2014 21836 For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com AKTA
13. ding the AKTA start Operating Instructions Do not open any covers or re place parts unless specifically stated in the user docu mentation The system complies with the requirements for electro magnetic compliance EMC in Australia and New Zealand The system complies with applicable European direc C 6 tives This symbol indicates that AKTA start has been certified 2 by a Nationally Recognized Testing Laboratory NRTL R 7 i NRTL means an organization that is recognized by the UsTeD US Occupational Safety and Health Administration OS Intertek HA as meeting the legal requirements of Title 29 of the Code of Federal Regulations 29 CFR Part 1910 7 Labels concerning hazardous substances The following symbols on the labels concern hazardous substances Medning This symbol indicates that electrical and electronic equipment must not be disposed of as unsorted munic ipal waste and must be collected separately Please contact an authorized representative of the manufac turer for information concerning the decommissioning of equipment AKTA start Product Documentation 29 0270 51 AD 31 5 Safety instructions 5 2 Labels Meaning This symbol indicates that the product contains haz ardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Sub stances in Electronic Information Products 5 5
14. e done in accordance with the Product Maintenance Procedures This product must not be disposed of as unsorted municipal waste and must be collected separately and handled properly after decom missioning AKTA start Product Documentation 29 0270 51 AD 35 5 Safety instructions 5 5 Declaration of Hazardous Substances DoHS Meaning SJ T11363 2006
15. e hazardous to humans if consumed Flush out the denaturated alcohol before assembling testing or integrating AKTA start into the intended process context WARNING To avoid hazardous situations when working with AKTA start take the following measures for personal protection Spread of biological agents The operator has to take all necessary actions to avoid spreading hazardous biological agents in the vicinity of the equipment The facility should comply with the na tional code of practice for biosafety CAUTION To avoid hazardous situations when working with AKTA start take the following measures for personal protection e Always use appropriate personal protective equipment during operation and maintenance of AKTA start Spillage hazard When using KTA start use personal protective equipment like goggles lab coat protective shoes and gloves to avoid any circumstances of spillage e Cutinjuries The tubing cutter is very sharp and must be han dled with care to avoid injuries e Hazardous substances When using hazardous chemical and biological agents take all suitable protective measures such as wearing protective glasses and gloves resistant to the sub stances used Follow local and or national regulations for safe operation maintenance and decommissioning of the equip ment AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 1 Safety precautions Personal protection 24
16. ed could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTICE e NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A note is used to indicate information that is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures 6 AKTA start Product Documentation 29 0270 51 AD 1 Introduction 1 2 Associated documentation 1 2 Associated documentation Introduction This section describes the user documentation that is delivered with the instrument and how to find related literature that can be downloaded or ordered from GE Healthcare Life Sciences User documentation on the CD The user documentation listed in the table below is available on the AKTA start User Documentation CD A display help is available from the Instrument Display Documentation Main contents AKTA start Operating Instructions Instructions needed to install operate and maintain AKTA start in a safe way AKTA start Instrument Display Help Dialog descriptions of the functionality menu for AKTA sta
17. ity flow cell is provided with a temperature sensor that measures the tempera ture of the liquid in the Conductivity flow cell Note The buffers used should be within the conductivity range of the instrument 0 to 300 mS cm Outlet valve A 3 port valve that is used to direct the flow to the Fraction collector or to Waste AKTA start Product Documentation 29 0270 51 AD 15 1 Introduction 1 4 Process and instrumentation design Process and instrumentation design 1 4 Illustration of the flow path The illustration below shows the flow path for AKTA start The flow path contains Pump Mixer Valves and UV Conductivity and Pressure monitors The individual instrument modules are presented in the table below For a detailed description of the modules see the AKTA start Product Documentation Description Description Buffer valve Injection valve manual Mixer Column Sample valve UV Monitor Pump Conductivity Monitor Pressure sensor Outlet valve Wash valve Fraction collector AKTA start Product Documentation 29 0270 51 AD 16 2 General specifications 2 General specifications 2 1 System specifications Benchtop system Instrument Display and or UNICORN start USB 540 mm x 280 mm x 560 mm 8 kg 100 to 240 VAC 50 60 Hz 95 VA Fast blow glass tube type F5AL250V IP21 PTFE tubing length 100 cm i d 1 6 mm 5 16 24 UNF connections PEEK tubing Length 15
18. l of Pollution caused by Electronic Information Products SJ T11364 2006 Symbols used in pollution control label Meaning This symbol indicates the product contains hazardous materials in ex cess ofthe limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Sub stances in Electronic Information Products The number in the symbol is the Environment friendly Use Period EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating conditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintain the declared EFUP the product shall be operated normally according to the instructions and environmental conditions as defined in the product manual and periodic maintenance schedules specified in Product Maintenance Procedures shall be followed strictly Consumables or certain parts may have their own label with an EFUP value less than the product Periodic replacement of those consumables or parts to maintain the declared EFUP shall b
19. ltubes 12 x 75 mm Fraction volumes 0 5 to 15 ml Flammable liquids NO Delay volume UV to Dis 0 49 ml default penser head Dimensions W x D x H 270 x 280 x 285 mm Weight 5 Kg AKTA start Product Documentation 29 0270 51 AD 21 5 Safety instructions 5 Safety instructions About this chapter This chapter describes safety precautions and emergency shutdown procedures for the product The labels on the system and recycling procedures are also described See page 22 29 32 34 55 In this chapter This chapter contains the following sections Section 5 1 Safety precautions 5 2 Labels 3 3 Emergency procedures 3 4 Recycling procedures 3 5 Declaration of Hazardous Substances DoHS 5 1 Safety precautions Introduction AKTA start is powered by mains voltage and handles liquids that may be hazardous Before installing operating or maintaining the system you must be aware of the hazards described in this manual Follow the instructions provided to avoid personal injuries or damage to the equipment The safety precautions in this section are grouped into the following categories e General precautions e Using flammable liquids e Personal protection e Installing and moving the instrument 22 AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 1 Safety precautions Do not operate AKTA start in any other way than described in the AKTA s
20. m Fingertight connector 1 16 2 2 Environmental ranges Parameter Storage and transport temperature range 25 C to 60 C Chemical environment 20 to 80 non condensing Relative humidity 2 5 Operating range Parameter Operating temperature range 4 C to 35 C Relative humidity 20 to 80 non condensing 2 4 Technical specifications 2 4 1 Pump Parameter Pump type Peristaltic pump Single channel four roller pump head with low pulsation 18 AKTA start Product Documentation 29 0270 51 AD 2 General specifications 2 4 Technical specifications 2 4 1 Pump 0 5 to 5 ml min operating range 10 ml min Wash flow e Accuracy Flow rate gt 1 ml min 15 Flow rate gt 1 ml min 10 e Precision Flow rate gt 1 ml min 15 Flow rate gt 1 ml min 10 Condition 0 8 to 2 cP and fresh pump tubing 0 to 0 5 MPa 5 Bar 0 6 to 5 cP Static mixer 0 4 ml Valves Buffer Sample Wash and Outlet Solenoid type switch valve 5 ports e Buffer valve and Sample valve 2 in 1 out e Wash valve and Outlet valve 1 in 2 out Parameter Flow rate Flow rate specifications Pressure range Viscosity range 2 4 2 Mixer Parameter Mixing principle Mixer volume 2 4 5 Parameter Type No of ports 2 4 4 Injection valve Parameter 19 Rotary type manual valve Sample injection through Loop Type
21. nformation Read before operating the product All users must read the entire Operating Instructions before installing operating or maintaining the product Always keep the Operating Instructions at hand when operating the product Do not operate the product in any other way than described in the user documentation If you do you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Intended use AKTA start is a liquid chromatography system used for preparative purification of proteins at laboratory scale The system can be used for a variety of research purposes to fulfill the needs of the users in the academia and in the life sciences industry AKTA start is intended for research use only and shall not be used in any clinical proce dures or for diagnostic purposes AKTA start Product Documentation 29 0270 51 AD 5 1 Introduction 1 1 Important user information Prerequisites In order to follow this manual and use the system in the manner it is intended it is im portant that e you understand the concepts of liquid chromatography you have read and understood the Safety instructions chapter in the AKTA start Product Documentation Safety notices This user documentation contains safety notices WARNING CAUTION and NOTICE concerning the safe use of the product See definitions below WARNING WARNING indicates a hazardous situation which if not avoid
22. pear as a computer failure The run continues and you can restart the UNICORN start client to regain control AKTA start Product Documentation 29 0270 51 AD 33 5 Safety instructions 5 4 Recycling procedures 5 4 Recycling procedures Introduction This section describes the procedures for disposal and recycling of AKTA start Decommissioning of the equipment AKTA start must be decontaminated before decommissioning Follow local regulations for scrapping of the equipment Disposal general instructions When taking AKTA start out of service the different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances AKTA start contains hazardous substances Detailed information is available from your GE Healthcare Life Sciences representative Disposal of electrical components Waste comprising electrical and electronic equipment must not be disposed of as un sorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment 34 AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 5 Declaration of Hazardous Substances DoHS 55 Declaration of Hazardous Substances DoHS Introduction The following product pollution control information is provided according to SJ T11364 2006 Marking for Contro
23. rt only accessible from the Instrument Display AKTA start Maintenance Manual Detailed instrument and module descrip tions including instructions needed to maintain and troubleshoot AKTA start AKTA start System Cue Card A condensed guide to prepare and run chromatographic techniques on AKTA start AKTA start Maintenance Cue Card A condensed guide to handling routine maintenance operations and trou bleshooting AKTA start AKTA start Product Documentation 29 0270 51 AD 7 1 Introduction 1 2 Associated documentation From the Help menu in UNICORN start or on the UNICORN start DVD the following user documentation is available Documentation Main contents UNICORN start 1 0 User Manual Overview and detailed descriptions of the system control software designed for AKTA start which includes process pic ture map for real time monitoring method editor evaluation and adminis tration modules UNICORN start Online Help Dialog descriptions for UNICORN start only accessible from the Help menu Data files application notes and user documentation on the web To order or download data files application notes or user documentation see the instruc tion below Step Action 1 Go to www gelifesciences com AKTA 2 Click AKTA Lab Scale Systems 3 Select AKTA start from the list 4 Click Related Documents 5 Select to download the chosen literature 8 AKTA start Product Documentation 29 0270 51 A
24. start is placed in a cold room place the computer outside the cold room and use the PC Connectivity cable delivered with the in strument to connect to the computer e Keep the pump cover open when not using the system Open the peristaltic pump cover after you switch off the equipment This will enhance the life time of the pump tubing AKTA start Product Documentation 29 0270 51 AD 27 5 Safety instructions 5 1 Safety precautions Maintenance WARNING To avoid damage to person when performing maintenance on AKTA start follow the instructions below e Electrical shock hazard Do not open any covers or parts unless specified in the user documentation Except for the mainte nance and service described in the user documentation all other repairs should be done by service personnel authorized by GE Healthcare Life Sciences e Only spare parts and accessories that are approved or supplied by GE Healthcare Life Sciences may be used for maintaining or servicing AKTA start e Disconnect power Always switch off power to the instrument before replacing any component on the instrument or cleaning the instrument unless stated otherwise in the user documen tation Spillage Hazard Avoid spillage of fluids on the surfaces of the instrument which have cables plugs and other wirings Be careful if there is spillage of fluids on the tray while trying to remove the tray from AKTA start e NaOH is corrosive and
25. sure sensor reads the pressure in the flow path and senses overpressure A 3 port valve that is used to divert the flow path to waste The Wash valve switches automatically during the predefined cleaning procedure Pump wash In a manual run the valve can be set to the intended position by configuring the run parameters A 6 port manually operated valve that is used to transfer the sample loaded in the sample loop on to the column A sample loop is connected to the appropriate ports of the valve The valve is switched manually to positions e Load sample default to allow the loading of the sample into the sample loop e Inject to column to transfer the sample from the loop on to the column during a chromatography run The UV Monitor continuously measures the ab sorbance of the liquid in the UV flow cell at a set wavelength of 280 nm The UV flow cell has a path length of 2 mm AKTA start Product Documentation 29 0270 51 AD Function Pressure sensor Wash valve Injection valve UV Part 1 Introduction 1 5 Instrument view 14 1 Introduction 1 5 Instrument view Part Function Description Conductivity The Conductivity Monitor continuously reads the conductivity of the liquid in the Conductivity flow cell The conductivity is automatically calculated by multiplying the measured conductance by the cell constant of the flow cell The cell constant is factory calibrated The Conductiv
26. t follow the instructions below e Vents on KTA start To ensure adequate ventilation keep papers and other objects away from the vents of the instru ment e Any computer used with the equipment shall comply with IEC 60950 and be installed and used according to the manufactur er s instructions e Frac30 should not be connected or disconnected from KTA start when the instrument is powered ON WARNING To avoid personal injury when operating AKTA start follow the in structions below e Rotating the instrument Make sure that there is always at least 20 cm of free space around AKTA start to allow for suffi cient ventilation When turning or moving the instrument take care not to stretch or squeeze tubing or cables A disconnected cable may cause power interruption or network interruption Stretched tubing may cause bottles to fall resulting in liquid spillage and shattered glass Squeezed tubing may cause in crease in pressure or block liquid flow To avoid the risk of knocking over bottles always place bottles on the buffer tray and turn or move carefully e Hazardous chemicals during run When using hazardous chemicals run the System cleaning template to clean and flush the entire system tubing with distilled water before service and maintenance e Setting Check that the correct outlet size settings are used Make sure that tubing and fittings are properly connected and secured Make sure that the pressure limit se
27. tart user documentation Operation and user maintenance of AKTA start should be per formed according to the instructions described in AKTA start Operating Instructions and AKTA start Maintenance Manual Do not use any accessories not supplied or recommended by GE Healthcare Life Sciences Do not use AKTA start if it is not working properly or if it has suffered any damage for example damage to the power cord its plug or the Frac30 cable damage caused by dropping the equipment damage caused by splashing liquid onto it Fire Hazard Before starting the system make sure that there is no unintentional leakage in the instrument or tubing Explosion hazard To avoid building up an explosive atmo sphere when using flammable liquids make sure that the room ventilation meets the local requirements 23 WARNING Always follow these General precautions to avoid injury when using KTA start WARNING When using flammable liquids with KTA start follow these precau tions to avoid any risk of fire or explosion e System operation e Maintenance General precautions Using flammable liquids AKTA start Product Documentation 29 0270 51 AD CAUTION To avoid hazardous situations when unpacking installing or moving AKTA start follow the precautions below KTA start is filled with denaturated alcohol 5096 C2H5OH ethanol at delivery The denatured alcohol mixture can b
28. therefore dangerous to health When using hazardous chemicals avoid spillage and wear protective glasses and other suitable Personal Protective Equipment PPE CAUTION To avoid damage to person when performing maintenance on AKTA start follow the instructions below e Hazardous UV light Always switch off power to the instrument before replacing the UV flow cell e f hazardous chemicals are used for system or column cleaning wash the system or columns with a neutral solution in the last phase or step before maintenance 28 AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 1 Safety precautions Cleaning Keep the instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the instrument dry completely before use NOTICE 5 2 Labels Introduction This section describes the safety labels and labels concerning hazardous substances that are attached to AKTA start The instrument serial number is also visible from the instrument product label which is illustrated here Labels on AKTA start The illustrations below show the labels that are attached to AKTA start Description Placement Instrument label including safety symbols and speci fication Do not open any covers on the instrument This will void the warranty 29 29022094 KTA start Code no 29003181 Voltage 100 240 V Serial no
29. ttings are correct before starting the run AKTA start Product Documentation 29 0270 51 AD 5 Safety instructions 5 1 Safety precautions e System operation 26 5 Safety instructions 3 1 Safety precautions CAUTION To avoid personal injury when operating AKTA start follow the in structions below e Max weight on Buffer tray Do not place containers with a volume of more than 1 liter each on the Buffer tray The maxi mum allowed weight on the Buffer tray is 5 kg Large spillage Switch off AKTA start and unplug the power cord if large spillage occurs NOTICE e To avoid damage to AKTA start or other equipment when operating the instrument follow the instructions below e Keep UV flow cell clean Do not allow solutions containing dissolved salts proteins or other solid solutes to dry out in the flow cell Do not allow particles to enter the flow cell as damage to the flow cell may occur e Pre fill UV flow cell Make sure that the UV flow cell is pre filled with liquid before starting the system e Avoid condensation If KTA start is kept in a cold room cold cabinet or similar keep the instrument switched on in order to avoid condensation e Avoid overheating If KTA start is kept in a cold cabinet and the cold cabinet is switched off make sure to switch off the instrument and keep the cold cabinet open to avoid overheat ing Place the computer in room temperature If AKTA
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