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1. 9 1 Make sure that hand wheel is in the lock position when the instrument is not in use 9 2 Change the broken lamp accordingly 110V 12W pay attention to safety when doing the excharige 9 3 Change the broken lamp accordingly 110V 8W pay attention to safety when doing the exchange 9 4 The elements of the electric system such as control panel electric control box require expert maintenance 9 5 The cooling system mainly consists of compressor condenser filter and evaporator The condenser with fan may accumulate dust after long usage this can affect the cooling function It is necessary to open the side ventilation board and use brush to clean the dust on the projecting vanes of condenser or use high pressure air to blow it clean 9 6 Frequently clean the constant temperature chamber to keep it clean Remove blade before cleaning 9 7 There are 5 fuse seats at the rear of the instrument from left to right models and functions are as following F1 F2 F3 F4 F5 5A 1A 1A 3A 3A power UV lamp lamp extractor peltier If broken please change them according to above model 10 Clean and maintenance These machines are precision instruments Pay attention to cleanliness maintenance and repair work Regularly clean and disinfect 10 1Daily cleaning work or freazing chamber Conduct the following steps before cleaning each time Take out ai specimens from freezing2. cover extractor hole to keep the freezing chamber enclosed to avoid moisture entering chamber 8 Troubleshooting Problems Caus s Heinedies Tissue cracking Select thinner tissue The freezing time is not quick enough Tissue specimen off Apply more embedding material The cutting tissue is soft i The iemperature of the specimen disc is low Tissue moves across blade bui without slicing Ensure that the blade is fastened and seated well Fix the specimen clamp securely Check that the the specimen disc is securely fixed on the clamp Increase the blade angle Tissue section rolling Temperature is not low enough chooser lower temperature Blade is not sharp change to new one Check the edge of anti rolling plate if broken change to new one Anti rollplate is not clean Clean with alchohol Check the gap between the anti roll plate and the blade adjust the angle of blade Increase the height of the anti rolling plate Check the angle of the anti roll plate and the blade Tissue becomes soft when cutting Prolong the refrigeration time of the anti roll plate and the blade Tissue section stick to the anti rolling plate Use short hair brush to clean the anti roll plate Prolong the refrigeration time Tissue section overlapping Clean the blade with camel hair brush The clearance angle between the blade and the anti roll plate is too narrow V
3. mode Left side LED displays F1 1 Front F1 stands for first mode the other 1 stands for switch on mode The lights from Mon to Fri all light up concurrently That means the instrument is on the working temperature From Monday to Friday instrument goes into stand by mode on the other time Under this condition if D button is pressed it does not function Hour button H and minute button M are used to adjust hour and minute respectively When in programming ic mode LED right digit will flash Then press again to go into switch off mode Left side LED displays F1 0 F1 this is the same function as above F1 0 stands for switch off mode Switch off programming method is same as above 10 seconds after finishing programming the instrument returns working state 6 6 3 Programming the working time for each day Press button three times continuously indicator lights up and unit goes into time programming Left side LED displays F2 1 first 2 stands for second time programming mode 1 at the end stands for switch on mode User may program different time for every day within one week If sigle light is on among Monday to Sunday user may program that day working time Press D to change among days Hour button H and minute button M are to adjust hour and minute respectively When under programming LED flash with right digit user then neess to press function as above 0 stan
4. 200ml 10 formalin or other sterile solution into the barrel in advance The disposal of the waste shall follow all the related regulations of the hospital 6 9 Left side control panel Refer to Fig 3 6 9 1 Specimen forward and backward feed Specimen forward and backward feed is or user fast move specimen position If user press W button specirien goes to home position in normal speed press button specimen N l goes forward in normal spesd Press L i button specimen moves to home A position in fast ne J speed press outon moves to opposite direction in fast speed Press y button to cancel moving conmimand 6 9 2 Section Trim thickness selection i Fig 9 is shows section trim control Above LED displays the thickness value of section trim SECT If SECT lights up LED displays section thickness TRIM TIS otherwise is trim thickness Press T S button per to change from section to trim one time or conversely Fig 9 Section Trim selection and display Section thickness range 1 90um default value is 64m 1 20um inium steps 20 40um in 2um steps 20 60um in 5um steps Trim thickness range 10 400um default value is 30pm 10 50um in 5um steps 50 100um in 10umsteps 100 400um in 50umsteps 6 9 3 Thickness selection Under current working mode adjust or to select section or trim thickness 6 9 4 Section Turn the hand wheel one rotation t
5. Cryostat Microtome RD 2298 Operation Manua Helpful Suggestions The User manual should be considered part of the instrument The user should read this user manual carefully before first installation and before operating the machine and place the manual close to the machine for reference The cryostat microtome must be operated by a trained operator Before operation please carefully read the operating instructions and familiarize yourself with the steps and processes Unit must be shipped in the upright position If the unit was shipped lying down or stored lying down then it MUST stand upright for 36 hours before use for all lubricating oils to enter the compressor Do not put instrument under extreme temperatures or a nigh humidity environment Failure to follow this procedure will cause severe damage Do not place instrument directly under sunshine or near air conditioning vents No other obstruction should be anund the instrument within a 30cm distance Check that your power outlet is we i grounded Ensure that power supply is stable constant and aclequaie Compressor requires a start up current between 45 and 504 Tnereiore a professional electrical engineer should inspect the electrical circuit before installation in order to ensure the smooth operation of the instrument Requirements The irstrument should be switched on 24 hours every day Blade is sharp exercise extreme caution when changing it remove when cleaning the insi
6. al order are Freezing shelf area Freezing chamber area Time setting area and Special function area 6 4 Peltier element i Under a normal state the peltier element is off LED ol shows freezing shelf temperature Press T button indicator light comes on and peltier element Zegins to Rs J work to cool down the freezing staticns LED displays L15 which means peltier eiament will work 15 minutes Fig 6 Peltier element The countdown of the ramaining cooling time is now permanently displaved The Peltier element turns off automaticaliv aiter 19 minutes Once the remaining cooling time disc aved is 4 minutes the figure 4 is followed by a point 4 At this stage ihe Peltier slement may be deactivated by pressing peltier button again Once deactivated the display indication returns to PE pressing the button during the peltier element working process will stop peltier freezing immediately 6 5 Temperature setting Compressor will start to work after turning on power switch allowing the freezing chamber to cool down depending on the interval betwen turning off and on if switched within 3 minutes the instrument requires 3 minutes for compressor to start again Freezing chamber temperature is shown and set by temperature set area as Fig 6 Normally LED displays temperature of sensor in the freezing chamber Freezing chamber temperature displayi and setting System def
7. ault temperature is 22 C When the mE instrument is under idle state press A to increase M Aly temperature press to decrease temperature At present the unit s LED is flashing to display set value When set to desired temperature wait about 15 seconds the instrument go back to normal state Fia 6 Freezina chamber temnerature User can adjust temperature within the adjustable range 6 6 Time setting Displays buttons on the panel stand for SUN Sunday MON Monday ae NEM Ee R E TUE Tuesday WED Wednesday C AN N THU Thursday FRI Friday PM SAT Saturday i AM Morning PM Afternoon W DAY Werking day H Hour ye w D H M Fig 7 Time setting area M ivtinite 6 6 1 Present time setting After turning on system default time it is noi correct then reset it When system is under idle state press D button to adjust cay light will flash correspondingly Press H to adjust hour the left two digit place oi LED will flash Press M to adjust minute the right two digit piace of LED will flash About 10 seconds after setting the instrument will return normal state A Note if setting time is over 10 seconds between two operations system will automatically return to normal state Below setting is the same 6 6 2 Programming working time a When under idle state Press O button once O symbol lights and is ready to enter programming
8. chamber Turn uo the specimen grip to the top and lock the hand wheel Remove tiie biade from the blade holder and put it back to the blade box Activate extractor to clean away scraps Empty waste tray Use alcohol or common disinfectants based on alcohol to clean baffle and brush shelf Collect waste cleaning liquid into wasted liquid barrel then dispose it Do not use organic solvents or any other aggressive substances to clean and sterilize the chamber Only use the cleaning agents specified in this manual such as alcohol or common disinfectants based on alcohol A Please do manual defrost before cleaning if chamber full of frost A If necessary the external painted surface can be cleaned with light du commercial housework cleaner Use wet cloth rub it until dry Do not apply xyler or alcoholic liquid eg glass cleaner 10 3 Spray disinfection The cryostat has to be disinfected after each daily use and cleaning Steps are as following Cool down freezing chamber temperature to 20 C Spray disinfectant after temperature decrease enough Wait for 15 minutes Wipe chamber off with tissue paper or dry cloth Reprogram temperature according to slicing requirement after disinfection 10 4 Blade holder cleaning If blade holder sticks contains impurities ang is sticking dismount and clean it as below Turn the adjustable handles in the iateral of rotator and draw it out from sideward Push the
9. ck to freezing chamber carrying on opposite procedure m Ensure that the clamp is in ine iowest position and then place the microtome slightly into the eft side of freezing chamber m Apply a drop of ow iemperature lubrication oil on the plastic coupling so that it may connect well wiih shaft B Use right hand to put handle of handwheel at the lowest position and universal clamp at the owest position too m Use left hand push microtome to right side turn the handwheel up and down to ensure proper alignment of plastic coupling and shaft Do not directly use heater to dry off freezing chamber This can cause damage to the cooling system
10. d has problem Display board is defective Compressor doesn t operate after turning on instrument more than 4 minutes Check the solid state reiay and change if necessary Compressor is darnaged No defrost Defective Soiencid valve Defective Soicnoid valve power supply Lamp does not come on Lamp is broken Laip wire has problem Chamber temperature stays same Defective Temperature sensor Freezing shelf temperature stays same Defective Temperature sensor chamber temperature can t reach to the programming temperature under running condition Lack of refrigerant please call to service to add some T404A and check for leaks Room temperature is too high keep more ventilation or install air conditioning to regulate chamber temperature at 25 C Bad ventilation on both sides of the instrument Move things away from both sides keep good ventilation Compressor is working without freezing Capillary tube pressure relief valve blocked call service Leakage of the refrigerant call service Fan working but compressor not Defective Compressar Freezing shelf temperature is unstable Water in the cooling distribution system ice blockage If the outiet of the compressor is sometimes hot and some times cold indicates water causing ice blockage Call to me nufacturer to vacuume and recharge system 9 Safety Maintenance and importance
11. d wheel speed 3 Fine adjustment of the anti rolling plate 4 Sharp blade edge correct blade angle 4 Frozen sections are just the opposite of paraffin slicing the frozen sections should not be cut in wide sections better to cut less wide sections so that the line of contact is shorter 7 9 Hand wheel lock To achieve operator convenience and safety we designed a handwheel locking function for the cryostat Lock handwheel at the top position not in use to avoid unnecessary harm f The locking position 3 8 o clock are at the top while gearing AS i p handle is facing to operator as showing in __ A Locking spanner the Fig 18 Rotary lever 7 gt TON E f N N aSo Fig 18 Hand wheel locker 7 10 Installing and Changing the Section Extraction Please take care of this precision instrument carefully It needs daily cleaning and regular mair tenance io achieve a long life for the instrument Suction nozzle Hose Filter Extractor install hole an tha inatviimant Fig 19 Extractor assembly Connect assemblies of extractor according to Fig 19 Assembly in Fig 19 is extractor install hole on the instrument Screw assembly into assembly to r connect with main instrument Press symbol to activate extractor Filter should be cleaned and changed regularly and be kept clear Other assemblies such as hose should be cleaned regularly as well A If extractor is not being used please use rubber cap to
12. de of the chamber Do not place blade anywhere with the cutting edge facing upwards Before changing specimens always lock the hand wheel and cover the blade edge with the blade guard In case of malfunction contact a qualified service technician Don t try to solve problem by yourself 1 Introduction The Semi automatic Cryostat Microtome is a national regulated 1st class medical device is used for rapid freezing of pathological sections of human and animal body tissue It can be widely used for pathological diagnosis analysis and research in hospitals medical colleges and by legal medical experts and propagation institutes The cryostat microtome is the eighth generation of our cryostat It utilizes advanced horizontal feeding The specimen chamber area is much larger than previous models The instrument consists of 4 major parts 1 1 The computer control panel is on the upper part This area displays the temperature and the general working conditions of the instrument 1 2 The center part is the constant low temperature chamber used to rapidly freeze the tissue and to conduct the cutting 1 3 Behind the chamber is the mechanical transmission and motor drive 1 4 The lower part is the compressor unit cooling condenser and evaporator 2 Scope of Application Pathological sectioning in order to study the histology of plant animal or numan tissue 3 Technical Parameters Section thickness range 1 90um Adjustable Incremeni i
13. ds for switch off mode Switch off programming method is again to go into switch off mode Left side LED distlays F2 0 front 2 same same as above 10 seconds after finish programming ine instrument returns working state 6 6 4 Programming the defrost cycie Press 9 l button that is in time control area Defrost indicator light is on and enter defrost programming Incicator lights of week on the top line go out currently It represents that program same defrost time every day W button is not available Hour button F and minuie button M are to adjust hour and minute respectively Programming time is defrost beginning time About 10 seconds after programming system return idle state 6 7 Function buttons e PA UV There are fast defrost stand by UV l sterilization lamp and illumination e O Y a g buttons in function area as shown in Fig 8 Fig 8 6 7 1 Locking key PJ When user press locking key time setting area is locked and could not be changed unless press it again 6 7 2 A T Fast defrost button Under normal freezing state it is in unlit Press to activate fast defrost function and indicator light is on system goes into fast defrost mode Press button again to cancel fast defrost indicator light goes out simultaneously Unit should then defrost withing a 6 minute time period A If system automat
14. ertical crack of tissue section Edge of the blade has a defect Foreign matter on the blade Tissue section narrows Adjust the clearance angle of anti roll plate and the blade evenly Tissue section broken Too low temperature of the tissue too long time freezing Blade is not sharp change to new one Blade sticking with sere impurity clean and brush Vibration when slicing Unfixed blade The angie of the vlade is too big or too small Specimen did not arrive at slicing temperature please continue to freeze it Specimen clamp is unfixed The specimen disc is not fixed well Cutting speed is too fast Uneven thickness of the tissue section Temperature is not proper Microtome did not dry completely Dull blade Angle is too small The specimen disc is not fixed Rotate handwheel but no feeding Microtome inside is not dry completely allow to dry Microtome itself damaged Please contact with technical service Defective Photoelectric sensor Defective stepper motor Defective stepper motor driver No display after power on Check the power line Reconfirm power to line Fuse blown Change fuse Can t reset after turning on instrument Defective Stepper motor Defective Stepper motor driver Lead screw or nut is broken Upper limitation Photoelectric sensor is defective No time and temperature display Connection cable between main board and display boar
15. f are peltier stations that the lowest temperature may arrive to 60 C Procedure of preparing specimen is as follows Cut specimen into proper size Put specimen onto freezing disc between layers of tissue freezing medium Put freezing disc into station on the freezing shelf Use hammer plat the specimen surface The specimen is frozen by low temperature quickly Move away hammer after it is frozen Insert freezing disc with specimen into hole of clamp on the microtome then tighten locking handle Adjust angle of knife holder and blade Rotate handwheel to cut specimen into sections 7 2 Microtome component introduction 1 Freezing disc locking handle Orientation adjustment handle Orientation lever Extractor hole Knife holder locking block Pak oD Specimen hole Inserted freezing disc Fig 13 Microtome parts introduction 7 3 Inserting freezing disc A Note Before inserting freezing disc please lock handwheel well Release freezing disc locking handle Insert the snaft of the freezing disc in the location hole of the specimen clamp Make sure that the shaft of the freezing disc is fully inserted The entire rear surface of the prism should contact well with the specimen clamp there should be no gap between them Lock ireezing disc locking handle and fix it Observe cutting angle if necessary adjust orientation Procedure for adjusting orientation Loosen orientation adjustable handle
16. h Fig17 Making flush between edge of blade and anti rolling plate The upper part of the anti roll plate should not contact the A tissue specimen 2 When the quality of the tissue section is not good check the edge of the blade check any segment of tissue on the plate 3 Avoid touching the front of the plate because high temperature will adhere the tissue section to plate 7 8 Section reference 1 When using the cryostat microtome controling the blade speed and correctly adjusting the anti roll plate are important factors in cutting high quality sections The proper speed of the blade can only be acquirea by practice and experience along with skillful control of the hand wheel Tine proper adjustment of the anti roll plate requires even more practice Sometisnes they interfere with each other and require careful adjustment 2 When freezing living tissue the water within the tissue will condense into ice the tissue will become hard The hardness changes with the temperature the lower temperature the farder the iissue becomes Different tissue reacts differently under different temperatures achieving high quality sections can only be obtained through practice cutting temperatures of the specimen tissue without fat and termalin shouid be between 13 C 23 C 3 For high quaiity sectioning pay attention to the following 1 Proper selection of working temperature in the chamber 2 Correct slicing operation and han
17. he system will cut the one piece of section one time and the thickness is the preset thickness Note When adjusting specimen quick fra ara nack movements the hand wheel must lock at the right position Refer to Fig 18 um extractor two control sections 6 10 Right side contrai panel Refer to Fig 3 6 10 1 Counter As left Fig 10 above LED displays number of total pieces and ENS thickness When n lights up total pieces is shown in LED on the contrast Zum lights up total thickness is shown Press M sm M button to change from total pieces to total thickness one time i or conversely Press C to clear all the numbers to 0 Fig 10 100 6 10 2 Use of vacuum extractor 50 F Vacuum extractor is designed for cleaning tissue scrap Press button on the right control panel to enable extractor motor Indicator light on the upper corner of symbol is on Move speed controller on the left side to adjust motor speed to choose suitable air volume Press again to disable it indicator light is off simultaneously 7 Mechanical introduction 7 1 Freezing shelf Freezing shelf Station oo Freezing disc Peltier station Specimen Fig 12 Freezing shelf description Freezing shelf with 20 stations is located to the left of freezing chamber The lowest temperature of freezing shelf may arrive to 40 C Two project stations in the front of freezing shel
18. ically enter fast defrost mode button lights up too Press it again to stop fast defrost and indicator light goes out 6 7 3 UV sterilize lamp ee UV is UV sterilization button Press it to make UV lamp ective press once more to stop it Indicator light is on or off correspondingly It is better to start to sterilize with UV lamp under stand by situation Note UV radiation is harmiul for human body When doing UV sterilize do not forget to close glass winaow and keep hands away from chamber Note Betore doing UV sterilization please remove all specimens and clean cnainoer first 6 7 4 Stand dy ea is stand by button Under normal state it is dark Press it one time system a goes into stand by mode Press it again to return normal working state Indicator light is on or off correspondingly Under stand by state freezing chamber temperature is maintained between 4 C 9 C to conserve electricity If system automatically enter stand by mode e button lights up too Press it again to stop stand by function and indicator light goes out 6 7 5 Illumination he Illumination button It is dual function button First press lamp is on second press lamp is off 6 8 Use of the liquid waste container Use the wasted liquid barrel to collect defrosted liquid tissue thawing solution flushing liquid and waste objects In order to prevent human and environmental hazard from the waste put
19. knife cig mp back wnich have knife clip and shift it out from the rotator Turn the eccentric rod handle in the lateral of the knife clap and draw it out from sidewara Clean all the assemblies of blade holder Dry off ai tie assemblies and apply a drop of low temperature lubricating oil on them Reassembly blade holder and put into freezing chamber A Before putting the blade holder back make sure all the assemblies are Ans 10 5 Lubrication Do low temperature oil lubrication for the following parts weekly 1 2 drop is well enough 10 6 Removal of the microtome After long periods of not being used inside of microtome needs lubrication please remove microtome out of freezing chamber Steps are as following Remove glass window Take all the accessories out chamber such as brush shelf left storage shelf right storage shelf freezing disc waste tray blade holder Use Allen key to loosen screw Take off plug of step motor Remove temperature sensor Slightly lift the microtome and pull it to the left to disengage the plastic coupling 5 connecting the axes Take the microtome out of the freezing chamber Loosen the screws on both sides of cover Take away cover and dry off microtome Yon may place microtome into oven at 40 C 50 C teniverature about 4 hour to dry it But you must apply low temperature iubricatici oil to cross roller bearings Please pay attention to below notico wien user put microtome ba
20. m Adjust orientation lever to choose best cutting angle Relock orientation adjustable handle 7 4 Blade holder position adjustment Fastening slide knob Clamping lever for locking blade Clamping lever for moving blade holder fro and back Turning lever to f z move blade holder m oo left to right i S Turn rotator to NY N adjust cutting ang QW of blade Anti rolling plate height knob Fig 14 Blade holder introduction Release anti clockwise blade holder lock handle on the left Fig 13 manually move the hoider fro and back and lock it at the desired position When the holder moves to the terminal point the whole blade holder can be taken out and put it back by reversing procedure Release counter clockwise blade holder lock handle on the right manually move the holder left and right and lock it at the desired position Note Remember to lock the blade holder to right position when doing forward or backward specimen adjustment 7 5 Change the blade Release clamping lever Oon the upper right to open blade clearance insert blade from left to right then lock the clamp lever Fig 13 VAN Note Blade is sharp beware when you change it 7 6 Adjust the angle of the blade Loosen the lowest adjustment handle on the right side of blade holder Refer to reference scale to turn rotator in order to choose best cutting angle of blade Retighten the adjustable handle 7 7 Adjust the anti
21. rolling plate a N pkarallel ade O OLS z N Anti rolling plate Cy sS i Knob f E PA A clamping anti rollir abe eT pie I f A NG N J s j A J f NN IEN fe h a Ai Ne LI 5 es M a Anti rolling plate height knob Using hexagonal wrench to blade Handle for replace scrap collector and slide make parallel Fig 15 Making parallel between edge of blade and slide The anti roir piate is made of Plexiglas Adjustment includes 1 Make the edge of the anti rolling plate parallel to the edge of the blade Fig 15 Insert blade and lock the clamping lever Lock two knobs Use hexagonal wrench to make blade and anti rolling plate parallel Fasten hexagonal screw Turn height turning knob to adjust height of anti rolling plate Note Please use Handle to replace scrap collector do not touch other knobs unless you want to adjust parallel 2 Adjust the anti rolling plate angle with the blade Fig 16 Anti rolling plate __Tissue Adjustable angle Angle of cutting lade edge Edge of plate and blade maatinna fliich gt Anti rolling plate hainht tiirnina kna
22. to ailicw proper air flow to the cooling unit There should be a minimum of 300mm space to facilitate ventilation and heat dispersion 4 5 he instrument uses a single phase AC power supply the voltage is 110 115V 11V 4 6 The instrument working ambier t temperature is 5 C 28 C If the temperature is above 26 air conditioning must be insiailed otherwise the life time of the cooling compressor will be shortered 4 7 Environment relative humidity should be no more than 80 c SSS i gt High temperature or humidity will influence freezing effect and shorten compressor life 5 Installation 5 1 Freezing chamber assembly Place left and right shelf in the freezing chamber outside of microtome Place the waste tray in the freezing chamber Install blade holder on the microtome base and lock it Insert blade to precool Place all tools needed for specimen preparation in the chamber Close glass window to enclose chamber 5 2 Connect Power VAN 6 Operation 6 1 General overview Check your power socket is well grounded Ensure that power supply is stable constant and adequate Compressor requires a start up current between 45 and 50A Therefore a professional electrical engineer must inspect the electric circuit before installation to ensure the smooth operation of the instrument Main Control Pane Left side AW Control Panel lt 3 Lo Main Right side Control Panel Moving Handle Hand
23. um Increment 20 Trimming thickness range 10 400um Adjusiabic Increment 10um Increment 20 Specimen retraction 2Cum Horizontal stroke 25mm Vertical stroke 59mm Specimen orientation 12 kK Y Z three directions Voltage 220V 10 Frequency 50Hz Power 650W Largest start up current 5 second 50A Chamber temperature 10 C 35 C Adjustable Freezing shelf temperature 50 C Number of freezing station 18 Peltier number 2 Refrigerant R404a 300g 10g Compressor oil 0 6L EMKARATE RL 22S ICI Dimensions Width No hand wheel 650mm Width With hand wheel 790mm Depth 700mm Height 1150mm Weight 140kg 4 Working Condition and Pre adjustment 4 1 The instrument is a movable floor type with 4 caster wheels Two front wheels can be up and down and are adjusted by turning knobs on the foot of the wheel These front wheels only function when the instrument needs to move After removing the packaging material put the front wheels on the ground and move instrument to the working position and secure it then disable the front wheels 4 2 The instrument should only be used after 4 hours of standing still to ensure proper lubricant distribution 4 3 Confirm the power supply is grounded triple socket then connect the instrument with the main power socket turn on the power switch at the back of the instrument the machine is now in operation mode 4 4 Areas on both sides of the instrument should be ciear
24. wheel Waste Liquid bottle Freezing shelf area Freezing chamber area Time setting area Special function area lt lt lt SUN NON TUE WED THU FRI SAT fo ZZ f o n e LS 6150 t Cryostat Microtome fam Fig 2 Main control panel Fig 3 Left side control panel Fig 4 Right side control panel 6 2 Turning the Insirument On Confirm the power supply is grounded triple socket then use attached power line to connect the instrument with the main power socket turn on the power switch on the right of the instrument the machine is in operation mode Main power is shown as Fig 5 on the right of the instrument The switch must be in the top position for on and in the bottom position for switching Fig 5 off Turn on the power switch on the right side of the instrument the machine is now in operation mode the sectioning arm automatic reset LED on the left side control panel should now display 06 um that indicates default section thickness is 06 um and present work mode is sectioning mode LED on the right side control panel displays to waiting for system reset After reset buzzer LED on the right control panel displays 0 to show total sectioning number 6 3 Function of main control panel Please refer to Fig 2 Main control panel is separated into 4 parts From left to right in sequenti
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