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Sorvall MX Plus Series Micro-Ultracentrifuge User Manual

1. xg S140AT S140AT Speed Control Accuracy Control Accuracy 50rpm 5 000rpm to maximum speed a Temperature 2 C set temperature is from 0 C to 40 C Control Display Accuracy Set SetSpeed 5 000 5 000 rpm to maximum speed in increments of 1 000 rpm to maximum 5 000 rpm to maximum speed in increments of 1 000 rpm in increments of 1 000 rom Vacuum Se Oil rotary vacuum pump and oil diffusion pump combined Ultimate vacuum below 0 6Pa 0 005 Torr Noise Level 45 dB A scale measured 1m in front of the instrument Maximum Heat 0 7 kW or less Dissipation into Room Cooling Method Method Thermo module Thermo module cooling CFC free CFC free screen a and touch sensitive LCD Operation Dimensions Width 440 mm Depth 520 mm Height 910 mm Height to top deck 790 mm Single phase 110 or 120 Vac 10 50 60 Hz 15 A 208 220 230 or 240 Vac 10 50 60 Hz 8 A Ambient temperature for performance 10 C to 30 C Ambient temperature for operation 5 C to 35 C RCF is an acronym for relative centrifugal force Please note that the LCD panel may contain a few dead or stuck pixels The voltage to be used is the one that you specified when purchasing the centrifuge CE The MX Plus Series ultracentrifuges satisfy CE marking requirements The CE marking is an international symbol which shows that the product conforms to EC directives Thermo Scientific MX Plus Series 6 1 Stan
2. I e Kc Scie Ta WS ne 2012 Thermo Fisher Scientific Inc All rights reserved Delrin TEFLON and Viton are registered trademarks of DuPont Noryl is a registered trademark of SABIC POLYCLEAR is a registered trademark of Hongye CO Ltd Hypaque is a registered trademark of Amersham Health As RULON A and Tygon are registered trademarks of Saint Gobain Performance Plastics Alconox is a registered trademark of Alconox Ficoll is a registered trademark of GE Healthcare Haemo Sol is a registered trademark of Haemo Sol Triton X 100 is a registered trademark of Sigma Aldrich Co LLC All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Thermo Fisher Scientific Robert Bosch StrafSe 1 D 63505 Langenselbold Germany Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete
3. 3 Press the Yes button on the deletion confirmation box Thermo Scientific RUN screen Menu Rotor Management Name S N Hours Runs Last run S1504T 01253 1000 run screen NENU Rotor Management S N Hours Runs Last run 0123 0 Are you sure you want to delete this rotor RAUN screen S I O01 Dec 2008 13 49 Rotor Management Name S N Hours Runs Last run Ho registered rotor Operation 2 a The Rotor Management screen appears e The deletion confirmation box is displayed MX Plus Series e The registered rotor Is deleted 2 65 2 Operation 6 Actual Run Timer setting Aun Screaen i llenu T Actual Run Timer Setting Actual Run Timer lt Actual Run Timer is enabled gt am i When you press the Enable button the timer starts Enable Disable counting after reaching the set speed lt Actual Run Timer is not enabled gt When you press the Disable button the timer starts Figure 2 21 Actual Run Timer setting screen counting immediately after the start of operation Press either the Enable or Disable button and make sure that the selected button is surrounded with a red frame Then press the x button or the ADMIN screen tab to store the setting 7 Date and time run SCENEN ilienwu 11 Dec 2008 6 50 Use this function to precisely set the current time setting Sen of the internal clock Month Hour Minute Set an exact date and time for RTC operation here Caa 11 8H 50 Figur
4. 5 Apply a thin coat of vacuum grease to the door seal O ring insert it into the groove and close the door Vacuum Pump If displaying the VACUUM alarm poor evacuation or other trouble occurs frequently the vacuum pump oil may deteriorate or the oil mist trap may be clogged or the vacuum pump may have a problem The vacuum pump oil should be changed once a year to prevent deterioration of the vacuum pump and clogging of the oil mist trap Note that the oil change frequency may differ depending on the use conditions and the environment Call a service representative when an oil change is required or any trouble occurs Oil for the vacuum pump is furnished with this centrifuge Store it in a safe place and give it to the service representative if requested 3 4 MX Plus Series Thermo Scientific Others 1 Storage period of service parts Service parts are kept in stock ten years after the discontinuation of production The term service parts means the parts that are necessary to ensure the correct functioning of the centrifuge Thermo Scientific MX Plus Series 3 5 o M Troubleshooting Contents e Alarm Indicators on page 4 3 e User corrected Problem on page 4 5 Thermo Scientific MX Plus Series 4 1 4 2 Be sure to read and keep in mind the following cautionary information before troubleshooting DANGER Before removing the cover or other components for troubleshooting always turn off t
5. Stop Status Stop Button Press the Run screen tab to return to the Run The Run screen appears screen You can view the applicable rotor names and their specifications In the MENU screen press the Rotor Catalog RUN SC EET icon The Rotor Catalog ROTOR CATALOG EA screen appears Hame SPEED RCFmax RCFavg ml E Previous Page button di S1SO04T 150000 501000 712000 a 1 00 gt Next Page button ER 314087 140000 1050000 883000 Gal fF H S140nNT 140000 752000 607000 2 00 ae Si20AT2 120000 650000 530000 i s 1 00 ip 5120aT3 120000 650000 S534000 idx 0 50 Number of tubes X ml wm p Mess If you want to view the MENU screen press e The MENU screen appears the x button or MENU screen tab If you want to view the Run screen press the e The Run screen appears Run screen tab 2 46 MX Plus Series Thermo Scientific Operation 2 Spin down Operation While you press this button and are holding down it the centrifuge accelerates up to the set speed at the maximum acceleration rate When you release this button it starts deceleration This feature is useful to remove the adhered samples on the interior walls of the tubes PULSE Operation e Touch Panel Operation Screen Displays and Notes In the MENU screen press the PULSE e The PULSE button is Operation Icon fi 19 Jan 2009 15 29 e oe ices displayed on the Run Remain Screen 0 SET 150000 SET 2 30 ROTOR PROGRAM fi PU
6. Basic Operation of the programmed Operation Feature If you create change or delete a program see below hers E Jen 7009 10 11 ti a TIME hhmm pi TEMP el e ol 0 00 25 0 set 150000 am 2 30 SET 4 0 SPEED rpm BOTOp ATG SET rpm S1504T START STOP SpEER accea PROGRAM RCF 1 N USER Lem wm Press the PROGRAM button Program No which stores the run parameters Is displayed PROGHAM Program nams sooorpe 3 Sppprpe 4 DHA STEP 1 ppppr pe Eme EA ul P 2 123456 TRS OOS EAT YU IOPASOPGHHJKZ If you load the run parameters into the centrifuge see below PROGRAM x HI Program namg Parameter a DNA STEI Eege pr pm Press the row of the desired program No Programmed operation possible Press the Run button to go back to the Run screen The loaded run parameters are displayed on the Run screen If you create or change a program C Press the Select button If you delete a program CH Press the Delete button Note You cannot create change or delete a program while in running Perform these operations while not in running However you can search the PROGRAM screen every time If you do not know the program No of the program which you need see section Programmed Operation 2 b 2 18 MX Plus Series Thermo Scientific Operation 2 1 Programming procedure for run conditions creati
7. H SILICONE RUBBER Oils Other Oleic Acid C Z C C DM NM DN DM WM WM DM DM MD NM YM C C C Se N N Gol c z c oi oi oi ol o A Gol Gol Gol Gol Go Mm WM WM WN Oxalic Acid Perchloric Acid 10 Perchloric Acid 70 Phenol 5 Phenol 50 WM MAM MM MM MN MW DM WM WM WM Z Cc WM C Z C C nan GO A a E A Col Go CT NM oi ol YM NM NM YM NM ol Z ns ol oi Ws Gol oi oi Gol oi WM Gol W ol CT Go ol a VITON Phosphoric Acid 10 Phosphoric Acid conc Physiologic Media Serum Urine WM Mm MM MD WM WM CM WM DM DM e Picric Acid I Pyridine 50 Rubidium Bromide Rubidium Chloride Mn nM Hin vn zZz Gol cn sl zZ nnn ce nan nnn Gol Z aj Gol wm oi ol Cc a POLYALLOMER MN Col MW Cl Gol WwW sl Gol c c c CC Gol Go sl nn Cl nm nm nm wm oi Cl oi oi CC si Go POLYCARBONATE MN Col WM Gol Gol WwW zZz Gol z ol zZ ol Gol nm Cl Gol nm ol wm wm ol nm ol oi oi ol Co a POLYPROPYLENE Gol nm mM Cl aj Go c c c Z nm wn Z Gol wn ol Gol Go Z Gol wm ol Gol wm ol Gol wm oi ol wm Go Z z JEC o z Cle amp j c SE SG cloj aj al E al aol E al z E ol z S z aj wm I l A A a c Eu aj z z c c c c z cl a ajaj r ajaj ajal zl E al ajl om EI SI E I LE I I I Fr L I I 41 I I LE I I I Nal aj aj c aj oaj c c z z c c c aJ aja I LE I I I E I Gol A ajaj z a z v c c c val c Ga a
8. Make a check then if you wantto Set itto 9 Set it to 7 change other settings press the area of the desired item If you do not want to change any other ACCEL o ACCEL E setting press the ENTER button Use the CE button to cancel input DECEL S DECEL 7 Setting range and units 1 9 free coasting FREE Operational Procedure This section describes the procedure for normal operation Note Before starting this device carefully read the operation manual for your rotor and make sure that you have selected the correct type of tubes and have entered the correct amount of sample Thermo Scientific MX Plus Series 2 11 2 Operation Step Touch Panel Operation Screen Display and Notes Turn on the POWER switch of the device e The touchscreen is displayed e The door is unlocked Install the quick setting rotor e Install the rotor securely on the crown It does not have to be screwed into 1 the crown e Always use a quick setting rotor see section Applicable Rotors Set run conditions e See section Setting Run Conditions and set run conditions Press the VACUUM button e The device starts evacuating the rotor chamber You can omit this step e Temperature control starts e The vacuum indicator on the VACUUM button displays the vacuum level in the rotor chamber 1 Low vacuum frente 1 bar 2 Medium vacuum a cona 2 bars 1 High vacuum TT 3 bars e f th
9. Note 1 Selecting the rotor is required to display and set an RCF value see section Displaying and Setting RCF 2 You can select your rotor in the Rotor Management screen if you registered your rotor in advance see section Administrator Admin Functions 5 Rotor Management 3 Selecting the rotor is required when the rotor lockout function is enabled see section Administrator Admin Functions 3 Rotor Lockout Displaying and Setting RCF This ultracentrifuge stores the maximum and average radii of each rotor in the internal memory Setting a speed causes this centrifuge to automatically calculate and display the RCF relative centrifugal force value while setting an RCF value causes the centrifuge to automatically calculate and display the speed This section describes the procedure for displaying and setting RCE 1 How to display an RCF value Step Touch Panel Operation Screen Displays and Notes 1 Check that the desired rotor is displayed in the ROTOR indicator of 18 Jan 2008 10 52 the Run screen Go to step 3 when the Zz T TME mnm desired rotor is displayed When the ere desired rotor is not displayed press 0 the ROTOR indicator to show the a I DS ROTOR indicator ROTOR ROTOR CATALOG screen r S1SOAT PROGRAM 2 Press the row of the rotor you want to see the RCF value and make sure that ROTOR CATALOG the pressed row turns blue BCC RFs AGF aN The
10. PROGRAM RCF DBCEL C USER F ai RCFn s 850000 SET BOT000 MX Plus Series 2 41 2 Operation Press the SPEED RCF button to turn the RCFmax RCFavg area to the SPEED area Mari Ric he SPEED value of the Ze em e gem rotor displayed in the ROTOR indicator is displayed on the lower part of the SPEED area This is the SPEED value for the RCF value entered in step 3 Note 1 To shift from the SPEED area to the RCF area press the button 2 This centrifuge is not capable of checking rotors Select the correct rotor Especially when you wish to determine a speed based on an RCF value selecting the wrong rotor and thus setting an RCF value exceeding the capacity of your rotor will cause the system to calculate and set a speed exceeding the maximum allowable speed However this ultracentrifuge has an overspeed detector which prevents an overspeed run 3 When you run this centrifuge at a speed determined based on an RCF value there may be occur a slight error of up to 2 between the set RCF value and the actual value because the speed setting is set in increments of 1 000 rpm User Login After logging in individual users can control the operation history of the centrifuge 1 Procedure for user login Step Touch Panel Operation Screen Displays and Notes In the Run screen press the USER area 19 Jan 2008 10 52 m apu hh mm n TEMP dd Remain USER area ROTOR ET P
11. accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history Original Instruction Manual 50136548 01 printed in August 2012 For Research Use Only Not for use in diagnostic procedures ThermofFisher SCIENTIFIC KONFORMITATSERKLARUNG DECLARATION OF CONFORMITY Name und Anschrift des Herstellers und des Bevollmachtigten fur die Zusammenstellung der relevanten technischen Unterlagen Name and address of the manufacturer and of the authorized representative to compile the relevant technical documentation Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg 37520 Osterode Germany Hiermit erklaren wir dass die nachstehend beschriebene Maschine Herewith we declare that the machinery described below Marke brand tt CCSS Modell Nr model no 50135642 50135643 50135644 Gultig ab Equipmentnr 41386606 Valid from equipment no mit allen einschl gigen Bestimmungen der Maschinenrichtlinie 2006 42 EG in Ubereinstimmung ist is in conformi
12. decelerate slowly to obtain sharp bands Pelleting using a fixed angle rotor Rapid pelleting of samples is possible the run time decreases Density gradient centrifugation 5 to 8 5 to 8 The sample and gradient do not reorient using a swinging bucket rotor Therefore mixing of the layers is less than that in the case of using a vertical rotor But It is safe not to accelerate or decelerate the rotor by selecting minimum time Thermo Scientific MX Plus Series 2 15 2 Operation How to use the OPTION Function PROGRAM button SPEED RCF button RTC button This ultracentrifuge incorporates a number of features such as step mode and other programmed running display and setting of centrifugal force and RTC real time control that can run the centrifuge at a required date and time Buttons for these features are displayed and specified on the Run screen 19 Jan 2009 10 11 ff SPEED rpm D TIME hh mm d Remain 150000 2 30 SET 150000 SET 2 30 SPEED 150000rpm ROTOR Fro button mica Si al PROGRAM gt e ACCEL 3 7 RCF DECEL 9 o m Figure 2 6 OPTION Buttons Programs stores and recalls run conditions This feature also offers a step mode operation a continuous run of multiple run conditions This feature is used to cause the system to automatically calculate and display an RCF value It also sets an RCF value and calculates the speed RCFmax indicates the maximum centrifugal force
13. displayed in the Rotor Management screen or not When the desired rotor is displayed press the row of the desired rotor and press the OK button When the desired rotor is not displayed press the Rotor Catalog button In the ROTOR CATALOG screen press the row of the desired rotor and make sure that the pressed row turns blue Then press the Enter button The desired rotor is displayed in the ROTOR indicator of the Run screen MX Plus Series ier 18 Jan 2009 10 52 TIME hh mm hh TEMP i Remain en SPEED rpm 0 SET 150000 ROTOR S150AT PROGRAM ler 18 Jan 2008 14 53 Rotor Management x Name S N Hours Runs Last run Ho registered rotor ROTOR indicator e The Rotor Management screen appears Nothing is displayed in the ROTOR indicator by pressing the Cancel button You can select your rotor in the Rotor Management screen if you registered your rotor in advance see section Administrator Admin Functions 5 Rotor Management ROTOR CATALOG Hane SPEER Lem EC 40000 1050000 883000 px 1 00 lt i gt TI S04 08T Eidel 752000 07000 fx 2 00 fp S120AT2 120000 BS0000 S30000 10x 1 00 whe ba St20aTS 120000 B60000 634000 ids D SO SPEED acceL s PROGRAM RCE DECEL 7 USERA F e The ROTOR CATALOG screen appears The pressed row turns from white to blue e The Run screen re appears ROTOR indicator Thermo Scientific Operation 2
14. 3150 AT 150000 agarad 711250 Gx 1 00 AD S140 AT ere Roter Catalog Hame SPEED ACKmax 140000 10456684 082296 10x 1 00 L s120 aT2 120000 ue 643826 820190 10x 1 00 fp 8120 aT3 120000 649826 599211 14x 0 50 f sizo vT 120000 bel ken 500237 410162 Bx 2 00 mee mc The PROGRAM screen re appears If you selected ST50AT these characters are displayed in the Rotor field Program m me DNA STEP User nane Thermo Scientific Rotor 51S0AT Parameter Thermo Scientific Operation 2 select run parameters as follows To enter a SPEED value If you want to enter a SPEED value as a parameter press the SPEED area and make sure that it turns blue Then press the Edit button e The PROGRAM entry screen appears To enter a RCF value If you want to enter a RCF value as a parameter press the RCFmax or RCFavg area and make sure that It turns blue Then press the Edit button RCFmax area RCFavg area Edit button Thermo Scientific MX Plus Series 2 33 2 Operation Step Touch Panel Operation Screen Display and Notes 7 Enter run parameters as follows a Make sure that the STEP 1 row is a The on screen keypad appears blue and then press the Edit button Using the on screen keypad enter the run parameters for STEP 1 see section Preparing Tubes Bottles and Rotor If you want to enter an acceleration or deceleration rate press the ACCEL DECEL but
15. In this example you set the above run conditions 2 through 6 set the start time for RTC operation to 6 00 April 8 and start the centrifuge You can make an identical setting by setting the finish time to 8 00 instead of setting the start time to 6 00 2 48 MX Plus Series Thermo Scientific Operation 2 1 How to perform an RTC operation Step Touch Panel Operation Screen Displays and Notes 1 In the Run screen enter the run parameters 08 Dee 2008 11 50 fr SPEED rpm apu hh mm wf Remain ser 150000 ser 2 00 ROTOR S150AT RTC button PROGRAM Press the RTC button If you want to set a run to start on a particular date and time press the Start Time button e To specify the run time centrifugation time do not set HOLD Enter the desired numeric value U If you want to set a run to stop on a particular Start time button date and time press the Stop Time button Stop time button e The Set RIC screen 08 Dec 2008 11 46 Minute x appears Enter the start time or stop time on the on screen keypad e You can enter the start time or stop time on the on screen keypad e f you want to change any of the Month Day Hour or Minuites setting press the area of the desired item and enter the numeric value e To set the hours use a number between 0 and 23 in the 24 hours system e Enter a time later than the current time When setting the stop time a
16. MU gt oO M S Ms S 8 S M U G M Aniline S S US Uwe Ms U U U U U UU SMUUSSSSUS Sodium Hydroxide lt 1 U MS S S gt M S S e MMS S amp S S amp S So S M SS U Cesium Acetate M S SSS gt G 2 2 e 9 gt Z oS aaa Mo D Thermo Scientific MX Plus Series A 1 CHEMICAL Cesium Bromide Cesium Chloride Cesium Formate Cesium lodide Cesium Sulfate Chloroform Chromic Acid 10 Chromic Acid 50 Cresol Mixture Cyclohexane Deoxycholate Distilled Water Dextran Diethyl Ether Diethyl Ketone Diethylpyrocarbonate Dimethylsulfoxide Dioxane Ferric Chloride Acetic Acid Glacial Acetic Acid 5 Acetic Acid 60 Ethyl Acetate Ethyl Alcohol 50 Ethyl Alcohol 95 Ethylene Dichloride Ethylene Glycol Ethylene Oxide Vapor Ficoll Hypaque Hydrofluoric Acid 10 Hydrofluoric Acid 50 MX Plus Series MATERIAL c c Z Go Go nm wn Go Z aj wn wn c Z al nm wm wn Z Eat Go wm Go C C c Z Z Z ALUMINUM c Mm N A W _ ANODIC COATING for ALUMINUM Mm WN WN WN WN WN Mi Mm el NMA MA NM CTC NM WM WM BUNA N C C WM C WM C WM WM C C Z cy WM C C C C C DM WM WM WM C C C C ox WM WM ND GO CELLULOSE ACETATE BUTYRATE K Cl CT anj Go WM C CH Loi C CG C WM N N N Gol WI a POLYURETHANE ROTOR PAINT Mm WN WN WN WN Mm WN WN WN WN WN cC Z al Col Gol Gol Gol a COMPOSITE Carbon Fiber Epoxy N WN WN WN WN Mm WN WN WN WN WN Gol Gol Z Z Z c Z
17. SET ROTOR alarm message appears this ultracentrifuge does not accept the CE or VACUUM button for 20 minutes to ensure safety Wait at least 20 minutes then press the CE button e f you want to clear this alarm when 20 minutes have not passed since this alarm message appeared see the ACTION of the alarm message POWER FAILURE rom MX Plus Series 4 3 IMBALANCE 1 Rotor is not properly balanced and e Check if the sample tubes exceed allowable imbalance level Tighten rotor cover or cap rotor sign of sample leakage SH 2 Rotor cover or cap is not properly e Tighten rotor cover or cap securely tightened INVALID SPEED Rotor speed is set higher than the e Set the speed within their permitted limits Check max speed of the maximum allowable speed rotor Set its max or lower speed again POWER FAILURE A power failure occurred while the rotor Unless the set run time has elapsed restart the run Running re started was rotating e H the instrument was automatically restored and the rotor is automatically or is rotating at set speed then let the run continue suspended Check status Refer also to section Emergency Recovery from Power Failure A power failure occurred while the rotor This ultracentrifuge does not accept the CE or VACUUM was rotating and the speed could not be button for 120 minutes from power failure detected after power returned If you are convinced that the rotor has co
18. are done by anyone else the ultracentrifuge warranty will be null and void 1 Place of installation 1 Locate the ultracentrifuge on a level vibration free floor capable of withstanding 350kg m 71 7 b ft 2 Ambient temperature for operation is 5 to 35 C If the room temperature rises above 35 C the temperature of the rotor may become too high Avoid installing the ultracentrifuge in direct sunlight 3 Keep the back of the instrument at least 20cm away from the wall We recommend you install the sides of the instrument about 15cm away from the walls However the centrifuge s performance will not be affected if its sides are about 5cm away from the walls provided that the walls are at heights similar to those of this ultracentrifuge Make sure that the air can circulate adequately around this ultracentrifuge Avoid installing this ultracentrifuge close to a heat generating device which might reduce this centrifuge s cooling capacity WARNING For operator safety maintain a 30 cm clearance envelope around the instrument and keep out that area while the rotor is spinning Do not store dangerous substances capable of developing flammable or explosive vapors on nor near the centrifuge Thermo Scientific MX Plus Series 5 1 5 2 Figure 5 1 Clearance for Ultracentrifuge 2 Electrical power requirement CAUTION Your ultracentrifuge can be damaged if connected to a wrong voltage Check the voltage before plug
19. cover and the same dimensions The following is the external view of the MX 150 Micro Ultracentrifuge Touchscreen display operation Safety cover Door Top deck External connection POWER switch 910 Front cover 490 Depth Including safety cover 520 Note This height is measured from level floor surface Figure 1 1 External View of MX 150 Micro Ultracentrifuge 1 2 MX Plus Series Thermo Scientific Structure Touchscreen The touchscreen with color liquid crystal screen is incorporated in the MX Plus Series You can set the run conditions perform the operation and display Run History Programmed Run and User Customizations Screens by pressing the screen The figure 1 2 shows the touchscreen Tabs of each screen 19 Jan 2009 10 11 Be SPEED rpm TM hh mm wf Remain 150000 2 30 SET 150000 SET 1 Display areas 2 30 da 7 RTC button ges SEH S150AT pm ne 6 PROGRAM button SPEED ACCEL 9 PROGRAM ae DECEL 9 8 USER button 3 VACUUM 5 STOP button button Indicator 2 Rotor indicator 4 START button Figure 1 2 Touchscreen Display at the normal Operation The following screen appears by pressing the SPEED TIME or TEMP button 19 Jan 2009 10 11 3 TIME hh mm Remain 9 On screen keypad Figure 1 3 Touchscreen Display when Setting the Run Conditions such as the Speed etc MX Plus Series 1 3 Thermo Scientific Functions of the Run Screen Displ
20. e Do not open the door while the rotor is spinning e Do not attempt to slow or stop the spinning rotor by hand e Do not incline or move the instrument while the rotor is spinning Do not place any object on the instrument or lean on the instrument Do not attempt to unlock the door forcefully while the rotor is spinning e For operator safety maintain a 30 cm clearance envelope around the instrument while the rotor is spinning Do not store dangerous substances capable of developing flammable or explosive vapors on nor near the centrifuge e Repairs disassembly and other modifications to the centrifuge are strictly prohibited unless performed by a Thermo Fisher Scientific authorized sales service representative e Do not use the others manufacturer s rotor without Thermo Fisher Scientific s permission e Always use a quick setting rotor for this ultracentrifuge The screw type rotors are inapplicable e Check the chemical resistance chart attached to the rotor and do not use any sample inapplicable to the rotor including the buckets Using such a sample could corrode the rotor including the buckets e Do not exceed the maximum rated speed of the rotor or buckets in use e Do not use corroded scratched or cracked rotor and buckets Check that the rotor and buckets are free of such abnormalities before operation e When using a swing rotor check that the buckets are properly engaged with the rotor pins before operation Wrong
21. for the maximum radius Rmax of the rotor which is used RCFavg indicates the average centrifugal force for the average radius Ravg of the rotor which is used Sets a start time or a finish time and runs the ultracentrifuge at a desired date and time Figure 2 7 Rotor Radius The above features can be used in combination Note To perform a combination of PROGRAM and RTC first set PROGRAM and then set the RTC Once RTC is activated you cannot change the run time You therefore cannot activate PROGRAM Programmed Operation When a centrifugal condition is to be used frequently entering the same condition every time you want to perform centrifugation is inconvenient This ultracentrifuge has a programmed operation feature that stores run conditions Storing run conditions which you often use allows you to call those conditions however often you may wish thus saving time in setting Even while the POWER switch is OF this centrifuge retains the conditions entered 2 16 MX Plus Series Thermo Scientific Operation 2 This centrifuge incorporates the program areas indicated below It has twenty memory areas and nine steps in each memory unit Running this machine with each memory unit retaining multiple steps will allow you to change the speed run time temperature and others while in operation Step mode operation Memory 20 Figure 2 8 Program Areas Thermo Scientific MX Plus Series 2 17 2 Operation
22. login is not required to start operation Figure 2 19 User Lockout Setting Screen Press either the Enable or Disable button and make sure that the selected button is surrounded with a red frame Then press the x button or the ADMIN screen tab to store the setting 3 Rotor Lockout Enable Select a rotor from the registered rotors to start operation It is impossible to select from the rotor catalog See section Administrator Admin Functions 5 Rotor Management ee a a Rotor Lockout Setting Rotor Lockout Disable The centrifuge is operable without selecting the rotor It is possible to select a rotor from the rotor catalog Enable Disable Figure 2 20 Rotor Lockout Setting Screen Press either the Enable or Disable button and make sure that the selected button is surrounded with a red frame Then press the x button or the ADMIN screen tab to store the setting 4 Japanese You can select whether to give displays in English or Japanese by pressing this button TT SA Japanese 2 60 MX Plus Series Thermo Scientific Operation 2 5 Rotor Management You can use and control the rotor data such as total operation hours and number of runs by registering your rotors in the centrifuge in advance It is recommended to use the rotor lockout e function see section Administrator Admin Functions 3 together in order to control the rotor data such as total operation hours and n
23. not want to change any other setting press the ENTER button Use the CE button to cancel input Setting range and units K rpm S 100000 SET 100000 Color Blue Co The last three digits are fixed l SPEED rpm 150000 SET 100000 Set it to 150 000rpm SPEED rpm 0 SET 150000 T Can be set to any value in the range from 5 000 rpm to maximum speed in increments of 1 000 rpm Running Time Temperature TIME TEMP ame hh mm Remain 1 00 SET 1 00 Color Blu EED UHN TEE EH Pressthe C button to turn the number of minutes blue For a continuous run press the Hold button 9 TIME hh mm Remain 2 30 SET 1 00 Set it to 2 30 2 hours 30 minutes C TIME hh mm Remain a 0 00 SET 2 30 Can be set to any value up to 99 hours 59 minutes in increments of 1 minute Color Blu N TEMP c 4 0 SET 20 0 Set it to 4 C Can be set to any value in the range from 0 to 40 C in increments of 1 C 2 10 MX Plus Series Thermo Scientific Setting Item Acceleration Deceleration ACCEL DECEL Lal E E Press the ACCEL 5 DECEL 5 button to show the on screen keypad Press the area of the desired item 3 Enter the desired numeric value with the on screen keypad L7 jis e Ile Ike ACCEL 5 O ab oO O _ ioe Cc O Wei ab Q A a a a E For free coasting press the FREE button
24. samples that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods e If there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales service representative e It is your responsibility to sterilize and or decontaminate the centrifuge rotor or parts properly before returning them to a Thermo Fisher Scientific authorized sales service representative Notice for an Earthquake Depending on the magnitude an earthquake might damage centrifuge If you observe some abnormality stop using the centrifuge immediately and ask for inspection by the Thermo Fisher Scientific service representative Vill MX Plus Series Thermo Scientific Oo 8 Description Contents e External View of Ultracentrifuge on page 1 2 e Structure on page 1 3 Thermo Scientific MX Plus Series 1 1 External View of Ultracentrifuge The MX Plus Series Micro Ultracentrifuges are floor standing types and require a small amount of floor space These two types of ultracentrifuges have the same external view except for the model name printed on front
25. the Normal or No Waiting Acceleration button and make sure that the selected button is surrounded with a green frame Then press the x button or the CUSTOM screen tab to store the setting Note 1 Select Normal mode when using the sample that is sensitive to temperature increases If you select No Waiting Acceleration the rotor does not wait until the vacuum reaches an medium level and it might cause the rise in the rotor retention temperature by the friction heat 2 If the rotor chamber has frost on it by the repeated operation it takes a long time to reach an intermediate vacuum In that case wipe it off with a cloth a sponge etc 3 Once you select the condition of the waiting for the vacuum Normal or No Waiting Acceleration your centrifuge memorizes this condition of the waiting for the vacuum If necessary change the condition of the waiting for the vacuum 4 If the low vacuum condition persists for more than one minute in the No Waiting Acceleration mode while the rotor rotates at the set speed the alarm message VACUUM ERROR is displayed and the rotor is stopped 2 54 MX Plus Series Thermo Scientific Operation 2 Administrator Admin Functions 2 You can set the administrative items such as language mainly Manager CAdmin In the MENU screen press the Manager Admin icon Then the ADMIN screen showing the eight functions is displayed as shown in figure 2 18 Tab of each screen RUN S
26. the color of the covers may be changed or the coating may be peeled off After use cover the centrifuge with a cloth to protect it from direct exposure WARNING e Your centrifuge must be grounded properly to avoid electrical shock hazards CAUTION e Do not place containers holding liquid in the rotor chamber or on or near the instrument If they spill liquid may get into the instrument and damage electrical components e If the machine will not be used for a long time turn off the main circuit breaker Safety against Risk of Fire Thermo Scientific WARNING e This centrifuge is not explosion proof Never use explosive or flammable samples or materials that chemically react vigorously Do not centrifuge such materials in this instrument nor handle or store them near the instrument MX Plus Series vil P Preface safety Notices Chemical and Biological Safety WARNING e Take all necessary safety measures before using samples that are toxic or radioactive or blood samples that are pathogenic or infectious You use such samples at your own responsibility e Take all necessary safety measures when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled and that more than one level of protection shall be provided in the case of materials of a higher group e If the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood
27. the registered This row of the desired rotor and make sure that operation is not required the pressed row turns blue for a new rotor Then press the Edit Hours button Name S N Hours Runs Last run Aun screen u NENU Hours b Enter the total operation hours of the rotor and press the Enter button Aun screen enw Rotor Management x Name S N Hours Runs Last run 0123 i000 Thermo Scientific MX Plus Series 2 63 2 Operation 3 Procedures for entering the total number of runs of the rotor are described as follows a EE e The total number of Rotor Management Name S N Hours SE runs of a rotor having 0123 1000 operation history can be a In the Rotor Management screen press the registered This row of the desired rotor and make sure that operation is not required the pressed column turns blue for a new rotor Then press the Edit Runs button b Enter the total number of runs of the rotor and press the Enter button ALUM screen Menu Rotor Management Name S N Hours Runs Last run a1 50A4T 0123 1000 Press the Run screen tab e The Run screen appears 2 64 MX Plus Series Thermo Scientific 2 Deleting a registered rotor Step Touch Panel Operation Screen Displays and Notes 1 In the ADMIN screen press the Rotor Management icon 2 In the Rotor Management screen press the row of the rotor which you want to delete and make sure that the pressed row turns blue Then press the Delete rotor button
28. the temperature in the rotor chamber Over Speed Sensor _ _ Stops the rotor when it rotates faster than the maximum Imbalance Detection Device allowable speed of the rotor Detects abnormal balance and Two sensors provided stops the rotor Figure 1 4 Rotor Chamber Safety Devices 1 Protection of Rotor Chamber Should the rotor spinning at high speed fails or comes off the drive shaft the safety of the operator is ensured by the thick protector Protection steel tube enclosing the cooling bowl figure 1 4 2 Imbalance Detector If during operation the vibration of the rotor becomes excessive due to serious imbalance or improper bucket setting the imbalance detector detects the situation and decelerates the rotor immediately However the ultracentrifuge is designed to tolerate imbalance associated with visual balancing it is equipped with an imbalance tolerant drive For more information on the balancing of rotors see section Preparing Tubes Bottles and Rotor 1 6 MX Plus Series Thermo Scientific 3 Door Lock System The chamber door automatically locks for safety while the rotor is spinning When the power supply is off the door remains locked The door can only be opened and closed when the rotor is at rest and the rotor chamber is vented To open the door in the event of a power failure see section Emergency Recovery from Power Failure 4 Over speed Detector This ultracentrifuge incorp
29. to 40 C in increments of 1 C Press this button to switch the area from the SPEED display to the RCF display Press this button to set the acceleration and deceleration e ACCEL Acceleration mode indicator Displays acceleration modes 1 through 9 e DECEL Deceleration mode indicator Displays deceleration modes 1 through 9 along with free coast F Press this button to display the ROTOR CATALOG where you can select the desired rotor Thermo Scientific Thermo Scientific VACUUM button button PROGRAM button When entering the deceleration rate FREE Is displayed in place of Hold e Press this button to turn the vacuum pump on or off When the vacuum pump is turned off the pressure in the rotor chamber will change to equal the atmospheric pressure While the rotor is rotating you can not turn off the vacuum pump e Temperature control starts as soon as the vacuum pump Is turned on The following four stages are displayed depending on the vacuum of the rotor chamber 1 r Atmospheric state The vacuum pump is not active 2 fruman Low vacuum The rotor waits at 5 000 rom until the vacuum reaches medium level 3 besen Medium vacuum NOTE If the sample is sensitive to temperature increases do not press 4 beameet High vacuum the START button until the chamber is at a high vacuum level Press this button to start rotor rotation If VACUUM is off pressing this button starts the vacuum pump and
30. value for the SPEED value entered in step 3 Thermo Scientific 2 How to set an RCF value Operation 2 Select the desired rotor and enter an RCF value and the centrifuge will calculate set and display the speed Shown below is the procedure Step Touch Panel Operation Screen Displays and Notes Check that the desired rotor is displayed in the ROTOR indicator of the Run screen Go to step 3 when the desired rotor is displayed When the desired rotor is not displayed press the ROTOR indicator to show the ROTOR CATALOG screen Press the row of the rotor you want to see the RCF value and make sure that the pressed row turns blue Then press the Enter button When SPEED is displayed in the area press the SPEED RCF button to show RCFmax RCFavg in the RCFmax RCFavg area Press the RCFmax RCFavg area and enter the SPEED value you want to convert into the SPEED value with the on screen keypad e g Rotor S150AT RCFmax 850 x 1 000 x g Press Ce and KE press the Enter button to enter the setting Thermo Scientific enw D rpm TIME hhmm Remain 501000 i2000 ap S14 AT 140000 1OS0000 883000 10x 1 00 ZB siaouT 40000 752000 607000 Ge 2 00 dip S120472 120000 B60000 S30000 IO 1 00 Lem KE E 120000 B60000 534000 idx D SO ROTOR indicator The pressed row turns from white to blue SR _ RCFmax area SET 901000 orem SET 50000 rem S1504T SPEED Orp SPEED acce g
31. 2 44 MX Plus Series Thermo Scientific Operation 2 Customize You can select rotor stop signal etc which enable you to easily run the centrifuge see xX section Customizing the Settings Customize Manager You can set the items which the administrator should set such as language see section Admin9 Administrator Admin Functions Manager Admin Press the desired icon Then the corresponding item is displayed Displaying the Run History and Loading the Information About Run Parameters The information about the run parameters of a previous normal operation can be stored automatically in the centrifuge and you can load It for a new run In the MENU screen Press the Run History Icon ES e The Run History screen Run History appears Date SPEED Time Temp Rotor _ 5000 0 01 25 0 S150AT Adain vee up Soreer Thermo Scientific MX Plus Series 2 45 2 Operation If you want to load the information about the run parameters for a new run press the ee column of the information which you want to SOc E rx _ Select the row load for a new run The row turns from white s 0ec 08 sooo a 01 25 ales to blue Check that the pressed row turns blue and press the Load for a new run button If you want to check the details of the selected information press the View Details button AUD SECEED Ss e The Run History Details Run History Details x SCPE hap pects Actual Speed 4770 Actual Temp 25 0
32. 3mL Sim 80 000 414630 23 323 452 580 30 Ann S70 AT 20x0 5mL mn mmm 307 052 31 31 0 op 561 30 45866 pu X 13 5mL D n 58 000 288 958 50 396 m 769 mm 45865 oui Guipmt n mmm 201 046 40 37 0 483 595 45 45979 S55 A 8 X13 5mL D n 55 000 259 839 56 396 m 769 mm 45540 mu Goin nn 50 000 209 438 pn 41 2 mi mn mm 45592 S45 A 12x 1 5m 18ml 45 000 124 858 D 324 438 552 A ii notor Cam e Volume ml Max Speed Max RCF k R Re Russ Tube Sau nominal rpm x g Factor mm mm mm Angle Swinging Bucket Rotors Vertical Rotors 45593 120 VT 8 x 2 0 mL 16 mL 120 000 500 237 L9 ER 25 0 31 1 0 Basic Operation WARNING Do not incline or move the instrument while the rotor is spinning Do not place any object on the instrument or lean on the instrument CAUTION 1 Do not press the touchscreen with a sharp pointed object such as a ballpoint pen 2 If abnormal sound is heard during the operation stop the operation immediately and contact a Thermo Fisher Scientific authorized sales service representative 2 6 MX Plus Series Thermo Scientific Setting Run Conditions This section will first describe the screen for basic operation the Run screen For the display at the normal operation and the display when setting the run conditions refer to section Touchscreen Run Screen The screen for displaying the specified setting and current status is called the Run screen Speed time and temperature areas display the current
33. Cl sl oi wm Z ol wm ol Gol Gool c c Z ol oi ol Go a DERIN ETHYLENE PROPYLENE So TEE NM DM ND ND nN nD ND WN WN WM Gol Mm WM WM WM wm GLASS Mm WN WN WN WN WN Mm Cc Wm WM N WM NEOPRENE Cl nl Gol Gol ni clini wm Cc al wm a WwW NORYL Mm CT MN WN TWN WN WN CC ol DW Gol Gol oi ol Gol Go Cl YM CT A Gol WM ol YM Go Gol Go oi Gol C c Cl soi ol oi ol w NYLON Cl Gol toi Gol Cl Cc Cc Gol Cc Gol Gol a Gol Go PET POLYCLEAR CLEARCRIMP C El El Cl E S SS MN Coin Go Go Go Cu toi Go Z Si E Ei to si nn Z Ei Go Go Go C C oi oi Z oi oi Co a POLYALOMER C C C C C nn wm C C Z Z SC a A oi a a POLYCARBONATE ES eh ee EEN EE ee a eS POLYESTER GLASS THERMOSET CH cy n Go WM CI WN Gol Z POLYTHERMIDE CH cy nn Go N N CI AN WM N aA Aa Co YM C ajl al NN NN YN Z al aj Z Ei ajl al anaj Z c oi anj Z na wm a a POLYRTHYLENE MN NAN Go YM Go C ajl Go Go Z E cl aj Z al aj Z ca aj al wm c c a oi Z oi a a POLYPROPYLENE c c Z a Gol Gol Gol a POLYSULFONE ZI WM WM WM GO C C sei C C oi nm wn Z C Z Z S j oi oi j POLYVINYL CHLORIDE NIAJ WN WN WN A A A Col ANA An W AM A DMA AIL MAI AIL AL AL AL al al al al nA a a RULONA TEFLON Z Z N N N oi W SILICONE RUBBER N z aj a Z Gi C C C Z Z Z Z z STAINLESS STEEL Nj Z aj ni nj anaj Co TYGON Mm WM WN aA C WN c cl Oo
34. LSE While you press the PULSE button and are e When the rotor stops the PULSE button disappears and the START button holding down it the rotor rotates and the STOP button appears e To cancel the spin down operation press the tab of the MENU screen tab and press the PULSE Operation icon again Note 1 While performing the spin down operation you cannot change run parameters until the rotor stops RTC Real Time Control Operation The MX Plus Series contains an internal clock allowing you to run the machine at a specified start or finish time for centrifugation This feature for running the machine at a specified time is called the RIC real time control feature The feature saves you the trouble of calculating the delay time for delayed start operation Explained below is how to perform an RIC operation with an example Example If you wish to install your rotor on the centrifuge under the run conditions listed below on the night of April 7 and to take out the samples around 8 00 the next morning 1 Rotor SISOAT 2 RPM 150 000 rpm 3 Separation time 2 hours 4 Control temperature 4 5 Acceleration mode 9 6 Deceleration mode 7 Thermo Scientific MX Plus Series 2 47 2 Operation 2h 150 000 Speed rpm START key ON 11 30 p m April 7 April 8 q 8 04 Automatic start at 6 00 a m Stop aroun Finish time 9 30 a m Figure 2 11 Typical RTC Operation
35. MX Plus Series N Col NA EI al a ajl NM WM C si sl aj nn wn C Z ajal wn aj Si Gol al nm Gol WM aj a Cc TITANIUM TYGON lt CIANA an WN N C aj sel n anaj Gi VITON A 3 CHEMICAL Lactic Acid 20 MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber Epoxy DELRIN ETHYLENE PROPYLENE GLASS PET POLYCLEAR CLEARCRIMP GO STAINLESS STEEL TITANIUM TYGON ro CELLULOSE ACETATE BUTYRATE NYLON Go POLYTHERMIDE N Butyl Alcohol N Butyl Phthalate N N Dimethylformamide Sodium Borate Sodium Bromide WM NM WM CC Z WM POLYESTER GLASS THERMOSET Sodium Carbonate 2 Sodium Dodecyl Sulfate Sodium Hypochlorite 5 Sodium lodide Sodium Nitrate Nj Nn nM Col Gol Loi Gol Gol c Cl z POLYVINYL CHLORIDE Gol Col MW Z Gol wn nm Gol Go c Col Z NEOPRENE Nj Nn MA Col WM WM Gol Go c Cc Z Go NORYL Gol DM WD WD ND ND WD WM C a W POLYRTIHYLENE Nj nM MA Col WM WM Gol WM Col Z Go POLYSULFONE MDM DA DNA AL NA AD ADA WD a a RULONA TEFLON Gel N N a Ge Cc Gel Ge N N Gel Gel N Gel Gel Gel Gel Gel Gel Gel Gel Gel Ge Cc Ge Ge Ge Gel Gel Gel Gel I WN I I I Gel Gel Gel Gel Gel N N N N Gel Gel Gel N Z N N N N Ge a Sodium Sulfate Sodium Sulfide Sodium Sulfite NILMA WM WM WM WN A A ANA GT anal WN Nickel Salts Oils Petroleum CC ol Go Gol Gol Col Z nm ol WM toi Z Z
36. N 1 Before using a rotor read the rotor instruction manual carefully 2 Do not use any corroded scratched cracked or otherwise damaged rotor or bucket Before operation always check if there is no corrosion and damage on the rotor surface 3 Before running a swing rotor make sure that each bucket is hooked on a pin securely Poor setting might seriously damage the instrument Even though all of them are not used make sure to install all buckets at any time Make sure that all the rotor buckets are of the same type 4 Some tubes and adapters are inapplicable at the maximum speed of the rotor Refer to the rotor instruction manual carefully 5 The S58A rotor S55A rotor and S50A rotor can handle sample whose mean density is 1 2 g ml or less Do not rotate this rotor at its maximum speed when using a sample whose mean density is over 1 2 g ml Refer to the rotor instruction manual Part No S999276 for how to limit the maximum speed when using a sample whose mean density is over 1 2 g ml 2 4 MX Plus Series Thermo Scientific Applicable Rotors Rotors applicable to the MX Plus Series are limited to the quick setting type illustrated below A quick setting rotor can be installed by merely placing it on the drive shaft crown in the rotor chamber Screw type rotors used in older models RC M100 RC M120 and RC M120EX Centrifuges are inapplicable to the MX Plus Series CAUTION 1 Always use an automatic securing rotor for th
37. PEECCECCER c The PROGRAM screen re appears Program nase User nane 4 Select a user name as follows a In the PROGRAM screen Press the i a The PROGRAM User name User name field Access level Last run Screen appears Administrator 0S Dec 2008 10 15 GGG Administrator 02 Dec 2008 10 09 Thermo Scientific Administrator n Enter em wm h b Press the row of the user name you want to select Then press the Enter button Sal b The PROGRAM screen Prodr as nans re appears If you selected a Thermo Scientific these User nane ees characters are displayed in Thermo Scientific stor the User name field Parameter Thermo Scientific MX Plus Series 2 31 2 Operation Step Touch Panel Operation Screen Display and Notes Select a rotor as follows a In the PROGRAM screen press the Rotor field b In the PROGRAM Rotor Management screen press the Rotor Catalog button c In the PROGRAM ROTOR CATALOG screen press the row of the rotor you want to select Then press the Enter button 2 32 MX Plus Series a The Program Rotor Management screen appears PROGRAM x Rotor Management Hane SIN Hours Rune Ho registered rotor Last run e f you registered your rotor in advance see section Administrator Admin Functions 5 Rotor Management you can select it in this PROGRAM Rotor Management screen b The PROGRAM ROTOR EE E CATALOG screen appears dip
38. ROGRAM 2 42 MX Plus Series Thermo Scientific Operation 2 In the User Management screen press the desired user name and make sure that the menu e he User Management User Management pressed row turns blue Then press the LOGIN User nane ES screen appears button Admin was registered as a user SE he pressed row turns name for this centrifuge before shipment from white to blue LOGIN button Enter the desired PIN on the on screen keypad and press the Enter button ch e The Login screen The PIN is the 4 digits number you entered on ES APPEALS the PIN registration screen see section Administrator Admin Functions 1 PIN field 1111 is the PIN of Admin registered as a user name for this centrifuge before shipment If necessary change the PIN Menu e The masked PIN is LOGIN PIN displayed Enter button The user name is displayed in the User area of the Run screen Menu 19 Jan 2009 15 10 e The Run screen T SPEED rpm 6 TIME hh mm p TEMP e re appears Remain SET 150000 SET 2 30 SPEED rpm ROTOR SET rem SISO4T ah ae USER area USER d Adain Note 1 User login is required to start operation when the user lockout function has been enabled 2 User Lockout You cannot operate cc CC as described in section Administrator Admin Functions the centrifuge without logging in Perform registration according to section Administrator cc CC Admi
39. Samples can be easily balanced visually see section Preparing Tubes Bottles and Rotor 8 This product spins very quietly and is thus well suited for personal use 9 A CFC free thermomodule cooling system is employed featuring a powerful cooling capacity Samples can be kept at 0 C at maximum speed when the ambient temperature is 30 C 10 The real time control RTC feature enables setting a start time or a finish time thus letting you run your machine at a desired date and time 11 Centrifugal force RCFmax and RCFavg can be displayed and set Note 1 12 Twenty varieties of nine stepped modes can be programmed for a wide range of applications such as step running 13 In addition to a door lock and an imbalance detector two independent microprocessors are incorporated for overspeed detection a dual CPU overspeed prevention mechanism for even greater safety Note RCF Relative Centrifugal Force Thermo Scientific MX Plus Series iii P Preface safety Notices Safety Notices Safety Reminders Carefully read and fully understand the following safety instructions e Operate your instrument according to the instruction manual e Be sure to observe the all safety precautions in the instruction manual and safety instructions on your instrument If neglected personal injury and or instrument damage can be caused e Ifthe equipment is used in a manner not specified by the manufacturer the protection provided by the equi
40. User Management box If you do not want to place the registered Admin Administrator 05 Feb 2009 14 44 screen Thermo user on the same access level with the TESR 2 User 05 Feb 2009 14 26 Scientific is added to administrator press User ower Administrator is displayed as the access level The screen display shows the case when placing the registered user on the same access level with the administrator Access level button PIN button e To change the access level or the PIN number after registration of the user name press the row of the desired user name and check that the row turns blue Press the Access level button or the PIN button to make a change For the difference of access level refer to section Administrator Admin Functions 2 e Jo delete the user name after registration press the row of the desired user name and check that the row turns blue Press the Delete User button to delete it Thermo Scientific MX Plus Series 2 59 2 Operation 2 User Lockout Enable User login is required to start operation You can RUN SC a See section Administrator Admin Functions 1 User User Management and User Login Soera In addition login of a user having the administrator access level is required to call up the Admin screen not set run conditions and start operation without logging User Lockout Setting A in Prior user registration is required Disable User
41. a oa Gol Gol ol oi oi Gol Gol ol z z z z val oi Goal w Gol Gol Go Cc nm Gol Gol wm c z sel Si wm wm Cc Cc oi wm 2 ajaj c c aj z a c c z Z a S aj Gr E r Gol Gol Ml Dl Gol Gol WD Gol Gol oi Gol Gol oi Gol Gol oi w Er ajaj a c a c c c c c cl oj z Gol Gol anaj Gol Gol al Cl GC WwW ol Z rm Gol Gol Col Go Gol Sai aj c z Nj nm Gol clala Gl al al cl CS al Sch Ech SS E Mm Col anj Cl Gol Loi Cc Gol Gol Go z Gol Gol Gol ou Cc El c Hi Si Se S S Hi Susi SC SF So Nj A aj c E Sucrose A 4 MX Plus Series Thermo Scientific 1 S 2 E 2 5 222 Seg2eeeese bs B2o2be2 2 82_ 2 28 BEE SSB eR 82 Sucrose Alkaline MS S e 2 D S S e oo Ha 8 SB OS 8 8 MS 8 S Polyethyleneterephthalate Thermo Scientific MX Plus Series A 5 Key S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc Suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable material Chemical resistance data is included only as a guide to product use No organized chemical resistance data exists for materials under the stress of centrifugation When in doubt we recommend pretesting sample lots A 6 MX Plus Series Thermo Scientific Warranty Thermo Scientific Thermo Fisher Scientific warrants that the Products will operate substanti
42. aft crown of the centrifuge once a month e Storing the rotor on the shelf is permitted if the shelf is taken necessary countermeasures against earthquakes not to drop the rotor CAUTION e Do not pour any solution such as water detergent or disinfectant directly into the rotor chamber and be careful not to spill the sample into the rotor chamber If you do so the bearings of the drive unit might corrode or deteriorate and it might cause vacuum failure e Do not operate the display panel Touchscreen using a ball point pen Safety during Installation and Maintenance vi MX Plus Series DANGER e Before removing the cover or other components for maintenance always turn off the POWER switch of the instrument unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock WARNING e For maintenance and repairing of the rotors tubes etc see the rotors and tubes instruction manual e After installation and before any test run this ultracentrifuge always needs the internal check by a Thermo Fisher Scientific authorized sales service representative e Repairs disassembly and other modifications to the centrifuge are strictly prohibited unless performed by a Thermo Fisher Scientific authorized sales service representative Thermo Scientific Electrical Safety P Preface safety Notices CAUTION e If the centrifuge is exposed to ultraviolet rays for a long time
43. ally in conformity with Thermo Fisher Scientific published specifications when subjected to normal proper and intended usage by properly trained personnel for a period of 12 MONTHS after shipment to the Customer the Warranty Period In addition the drive is warranted for 5 years after shipment to the Customer Thermo Fisher Scientific agrees during the Warranty Period provided it is promptly notified in writing upon the discovery of any material defect and further provided that all costs of returning the defective Goods to the Thermo Fisher Scientific are pre paid by the Customer to repair or replace at Thermo Fisher Scientific s option defective Goods so as to cause the same to operate in substantial conformance with the said specifications Replacement parts may be new or refurbished at the election of Thermo Fisher Scientific All replaced parts shall become the property of Thermo Fisher Scientific All consumable or expendable items are expressly excluded from the warranty under this Clause Thermo Fisher Scientific s sole liability with respect to equipment materials parts or software furnished to Thermo Fisher Scientific by its third party suppliers shall be limited to the assignment by Thermo Fisher Scientific to the Customer of any such third party supplier s warranty to the extent the same is assignable In no event shall Thermo Fisher Scientific have any obligation to make repairs replacements or corrections required in who
44. anual provided with the rotor Rotor Chamber CAUTION Do not pour any liquid such as water detergent or disinfectant directly into the rotor chamber and be careful not to spill any part of a sample into the rotor chamber If you do so the bearings of the drive unit might corrode or deteriorate and might cause a vacuum failure To maintain the rotor chamber 1 When the ultracentrifuge is not in use keep the rotor chamber ventilated 2 If the bowl is moist wipe it with a clean dry cloth or sponge 3 If the rotor chamber is dirty wipe it with a clean cloth wrung out in a diluted solution of a mild non alkaline detergent For sterilization wipe it with a cloth dampened with 70 ethanol Drive Shaft Crown Cabinet CAUTION Once a month clean the inside of the drive hole crown hole of the rotor and the surface of the drive shaft crown of the centrifuge If the drive hole or the drive shaft is stained or if foreign matter adheres to it the rotor may be improperly installed and come off during operation This part is very important because the rotor is mounted on it and the crown transmits the driving force to the rotor Before mounting a rotor wipe the outer surface of the crown with a soft cloth sufficiently dampened with water Always keep the top deck and the cabinet of the centrifuge clean to prevent dust and other materials from falling into the rotor chamber Wipe the top deck and the cabinet with a cloth or spon
45. ay areas SPEED area RCF area TIME area TEMP area button SPEED es ACCEL DECEL button Rotor indicator ROTOR 1 4 MX Plus Series These areas display various types of information The SPEED RCF TIME and TEMP areas display the current status in the upper part and the specified setting in the lower part For settings see section Setting Run Conditions e SPEED Speed indicator Upper part Displays the speed in increments of 10 rom when lower than 5 000 rom and in increments of 100 rom at 5 000 rpm or more Lower part Used to set and display a speed from 5 000 to the maximum speed in increments of 1 000 rom The lower three digits 1 10 and 100 positions display zeros Maximum speed MX 150 150 000 rom MX 120 120 000 rom For details on RCF see section Displaying and Setting RCF e TIME Running time indicator Upper part Displays the remaining operation time If the running time is set to HOLD the upper part displaythe elapsed time Lower part Used to set and display a range from 1 minute to 99 hours 59 minutes in increments of minutes and hours e TEMP Temperature indicator When the pressure in the rotor chamber equals the atmospheric pressure the temperature of the inside of the rotor chamber is kept at 25 C to prevent condensation Upper part Displays the temperature in increments of 0 1 C Lower part Used to set and display a temperature in the range from 0 C
46. be set for identification see section Administrator Admin Functions 8 The operation history data of the centrifuge can be exported to a USB flash drive If your centrifuge has a LAN communication option LAN communication start stoppage can be specified see section Administrator Admin Functions 9 Press the desired icon Then the corresponding item is displayed After setting press the tab of the desired screen 2 56 MX Plus Series Thermo Scientific Operation 2 1 User Management A User Management You can register up to 40 users with the system How to store register and how to change a user name are described below Step Touch Panel Operation Screen Displays and Notes 1 Press the User Management icon Then press the Add User button e The User User Management Silbe Management Last run Administrator OS Feb 2009 14 44 Admin whose PIN is 1111 has been registered in this centrifuge before shipment If necessary change the PIN 0S Feb 2009 14 26 User name box mm x button Ej e A screen to register a user name Is displayed Clear button Space button shift button Delete button Enter button e Press the Shift button to toggle case e Press the Clear button to delete all the characters in the user name box e Press the Space button to put one space after the last character in the user name box e Press the Delete button to delete t
47. ber Vacuum release knob Release wire of the door lock TR i iN A V Vu A d A d e A i Square hole of the frame Figure 2 29 Door Lock and Vacuum Release Knob 6 After taking out the rotor insert the hooks which are on both sides of the front cover into the frame and then fasten the front cover with the fastening screws Top deck Front cover ei Figure 2 30 Inserting the Front Cover Thermo Scientific MX Plus Series 2 71 2 Operation 7 Plug the power cord into the wall outlet 8 After the power returns turn on the power switch on the centrifuge Press the VACUUM button to turn on the vacuum pump for approximately 15 minutes and then press the VACUUM button to turn off the vacuum pump Then turn off the power switch on the centrifuge These procedures are effective ways of keeping the vacuum pump in good condition 2 12 MX Plus Series Thermo Scientific Maintenance Thermo Scientific MX Plus Series 3 1 3 2 Be sure to read and keep in mind the following cautionary information before maintenance DANGER Before removing the cover or other components for maintenance always turn off the POWER switch on the device unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock WARNING 1 If the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood samp
48. button to check the run parameters If you do not think that the selected program is the program you need press the x button to switch to the previous screen After checking the run parameters press the Run button if the selected program is the program you need STEP 1 3 In this case this program has three steps In the Run screen the run parameters for the first step in this program are displayed 5 Run the centrifuge under normal operation without making changes to the run parameters PROGRAM EN Mi Program name Parameter Nun 3000r pm 2 123956 TSS 0OWERTYUIOPASOFGHHIK2 S000r pm Program name User name Thermo Scientific Rotor Paranetor Operation 2 Edit button 2 120000 1 00 200 an a 100000 1 00 20 0 8 9 7 TI Pr 19 Jan 2008 10 52 i TEMP cl Remain 0 00 25 0 180000 SET 20 0 rpm ACTOR ATC fam S1SG4T START STOP Pag t rpa T TIME hhmm SPEED_ ACCEL 4 d PROGRAM BEE DECEL 9 DHA STEP Program name e The program name is displayed on the PROGRAM button indicator e f the STEP 1 3 is displayed on the PROGRAM button indicator this means that the program you have just called stores multiple run parameters steomode feature For details see section Step mode Operation e Run this centrifuge according to section Operational Procedure e f you make changes to the run parameters such as SPEED and TIME after calling a prog
49. centrifugal force RCF values from set speed CF It can also calculate reversely i e finding speed from Section such values Displaying and Settin RCF centrifugal force Go SN Ing value display setting Other features RCF value setting Spin down operation Spin down operation is useful to remove the adhered Section samples on the interior walls of the tubes Spin down Operation Run starts or completes at a required date and time A finish date and TC a time required a T Bross START section RTC Real Time button Control Operation 3 31 PM5 00 an Auen Time gt 4 RTC real time control operation 2 2 MX Plus Series Thermo Scientific Run Preparation WARNING 1 This centrifuge is not explosion proof Never use explosive or flammable samples or materials that chemically react vigorously Do not centrifuge such materials in this instrument nor handle or store them near the instrument 2 Take all necessary safety measures before using samples that are toxic or radioactive or blood samples that are pathogenic or infectious You use such samples at your own responsibility CAUTION Do not place containers holding liquid in the rotor chamber on the centrifuge or near the centrifuge If spilt liquid might get into the instrument and damage electrical and mechanical components Starting up this Machine Before setting run conditions display the Run screen Screen for setting run co
50. ces in electrical and electronic equipment please visit the website www thermo com WEEERoHS e Contents Chapter 1 UE 101 C1 a ee eee eee ee eee eee eee 1 1 Ee EE 1 2 EE 1 3 AUIS Ge E 1 3 Rore e ENEE 1 6 ee 1 6 Chapter2 Operation EE 2 1 IT E 2 3 Startas up CIS Machine ebion roroi aanne ea n a a a 2 3 Preparing Tubes Bottles and Rotor 2 3 EENG 2 5 Dasi ols ale eee rene rene a ee eee 2 6 EE EE 2 7 Operanonal POC CCS essien eneas iae aaa ERINRAR 2 11 Acceleration Rate and Deceleration Rate 2 15 How to tise the OP TION Pane tioncstccwosudsacescscenescnnesesetwackivncoosbbasacwsacdeesesaedeensandadese 2 16 eegene 2 16 ane 041814 8 ee 2 29 KE 2 37 IR e ROP aerarii e ener eee eee 2 39 Dees 2 42 Features of the MENU Screen 2 44 Displaying the Run History and Loading the Information About Run PPA AC CET ee 2 45 Eege 2 46 GEREENT OMe PaO sseni irens on ena EE E EERE a 2 47 RTC Real Time Control Operation exces yenovaiyenateecdeniececsauctctsacteatbeatazecekaaceaiads 2 47 Customizing the sc an cannon ere re nr ieee ere ene ere ten eee eee ee 2 51 Administrator Admin FUunctions cccccssssscccccssssssscccccsssssscccessessssscceseesesssess 2 55 Emergency Recovery from Power Failte siccccscceccteascsnapiecssisivevsdebaweteseessteieensesdceuiee 2 69 Chapter WOEN 3 1 FROG eenegen ebe 3 3 Dive e EE 3 3 CAD oss vse a E E EAEE TA EEA AEA AAE 3 3 Chomo r Eeer 3 3 eege 3 4 EE 3 4 EE EE 3 5 Thermo Sci
51. cient by Thermo Fisher Scientific It is your responsibility to bear the cost of sterilization or decontamination If you have any question please send e mail Note that Thermo Fisher Scientific cannot repair or inspect the centrifuge the rotor or the accessory unless sterilization or decontamination is completed CAUTION Do not perform any operation not specified in this manual If any problem is found on your centrifuge contact a Thermo Fisher Scientific authorized sales service representative Before removing the cover top deck or other component for troubleshooting always turn off the POWER switch of the instrument unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock This ultracentrifuge incorporates a self diagnosis feature that diagnoses the cause of any problem which may occur when you start the centrifuge or while in operation MX Plus Series Thermo Scientific Alarm Indicators If any trouble occurs this machine gives a buzzer sound and displays an alarm message in the message field of the Run screen to warn of the trouble i Jan 2012 8 15 SPEED rpm TIME hh mm TEMP T D d Remain 0 0 00 0 0 s 150000 BUS AR SPEED rpm SET rpm i i Alarm message SPEED ACCEL a RCF DECEL 9 N USER Figure 4 1 Displaying an alarm message 1 Responding to an alarm signal If an alarm message appears remove the caus
52. cl z a a om c c c Si z c z Eh Sl a mo u Cc Hi N C nN wm C C C C Z C a C C to c Ce NN Gol ND WM Gol NM WM Gol WM WM Gol Co VITON lt C c oi Co DN YN ND ND ND ND ND ND ND ND ND ND NN YN YN YN Zn Z oi c a WM WM WM W TITANIUM 1 A WN c c sl toi z Thermo Scientific CHEMICAL Hydrochloric Acid conc Formaldehyde 40 Glutaraldehyde Glycerol Guanidine Hydrochloride Haemo Sol Hexane lsobuty Alcohol Isopropyl Alcohol lodoacetic Acid Potassium Bromide Potassium Carbonate Potassium Chloride Potassium Hydroxide 5 Potassium Hydroxide conc Potassium Permanganate Calcium Chloride Calcium Hypochlorite Kerosene Sodium Chloride 10 Sodium Chloride sat d Carbon Tetrachloride Aqua Regia Solution 555 20 Magnesium Chloride Mercaptoacetic Acid Methyl Alcohol Methylene Chloride Methyl Ethyl Ketone Metrizamide Lactic Acid 100 Thermo Scientific MATERIAL mM nm C Z nm Cc ALUMINUM SCH ZE co E CS Sl al Cc Se Cc om SE S Sch Sal Cc El Et Sa EI GS C aj c E aj cl ajaj MN Col c aj Gol Z Z ANODIC COATING for ALUMINUM N NM C A ANA AN WN BUNA N N c CELLULOSE ACETATE BUTYRATE C A C Aa POLYURETHANE ROTOR PAINT Mm MN WN WN WN ANJ A Mm ND WN WN WN WN N N aj Z ana ana GO a c COMPOSITE Carbon Fiber Epoxy CcC ajl anaj anaj Z anj Gi NLAJ WN WN AN WN WM MM WM WM Z WM Go Si a n v
53. dards concerning these directives are as follows e Product safety EN 61010 1 and EN 61010 2 020 Environment requirements e indoor use e altitude up to 2000 m e maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Pollution degree 2 Installation category II e Electromagnetic compatibility EN 61326 1 EN 61000 3 2 and EN 61000 3 3 6 2 MX Plus Series Thermo Scientific Supply List The items below are those supplied with the ultracentrifuge Aen ne PartNo Units Supplied item Drawing No e Power Cord Assembly S204730 a i 2 5 m long Tool Box C S205074 _ H Supplied in 1 Vacuum Pump Oil S410357 1 Container R 2 Instruction Manual 50136548 Thermo Scientific MX Plus Series 7 1 e En Chemical Compatibility Chart gt me ge E 3 e a e p E a See aa S ER O 2 mercaptoethanol gt 5 U S MS SUS SUSS gt gt gt Oo US So onoo D Acetaldehyde S U U U MU U UMM MS U S U Acetone MS U S UMS S U US US UUUS S UUS MMS U U Acetonitrile S SU SIMs e Us UM YU U Se MU Des e E A Alconox US es SS oS Se SS SS DO OMS SS SS So SD SS Ss SU Allyl Alcohol U S S 8 839 MS 8S 8 MS 9 Aluminum Chloride U U SSSSUSSSSISMSSSS SSS a SMU US 8S Formic Acid 100 SMU U U SMUUSS U S U S U Ammonium Acetate gt S U SS D 5 gt oo eo oe noUs OC dE dE DD D Ammonium Sulfate UMS S S USSSSSSSSS SSSSSSUSSU Amyl Alcohol S
54. e 2 22 Setting at Time screen Press the area of the desired item Year Month Day Hour or Minute Check that the pressed area turns red and enter the date and time on the on screen keypad If you want to change the other items press the area of the other items You can also turn the desired area to red by pressing the gt button and lt button If you do not want to change the other items press the Enter button on the on screen keypad 2 66 MX Plus Series Thermo Scientific Thermo Scientific Operation 2 RUN screen Menu 11 Dec 2008 8 55 REIS Example Year Month Day Hour Minte Month April Day 8th Time 8 58am Figure 2 23 Setting at Time Screen If you want to change the setting of the other items on the ADMIN screen press the ADMIN screen tab 8 ID Contact When you have two or more centrifuges you can set the centrifuge ID for each centrifuge for T identification ID Contact 19 Jan 2009 18 45 Machine ID JAE Service contact Make a memo of your service Set the centrifuge ID Renee person s contact address 4 digit number Figure 2 24 Centrifuge ID and Service Contact Setting Screen Press the Machine ID field E CH Press the Service contact field muy screen Nenu 18 Jan 2008 16 45 13 Jan 2008 18 45 Machine ID Figure 2 25 Centrifuge ID Entry Screen Figure 2 26 Service Contact Entry Screen MX Plus Series 2 67 2 Operation 9 USB LAN communication This function is
55. e of the trouble as described below and press the CE button You will then be able to resume your centrifugation authorized services representative WARNING Unspecified repairs remodeling or disassembly of the centrifuge that is not listed below is strictly prohibited by any person other than a Thermo Fisher Scientific If the alarm message persists even after you have done what is specified below contact service representative to order a repair CLOSE DOOR The VACUUM or START button has been Close the door completely pressed with the chamber door left Press the VACUUM or open START button VACUUM ERROR 1 Required level of vacuum cannot be Wipe off moisture inside reached the rotor chamber or 2 After a satisfactorily high level of check a sample leakage vacuum was reached it lowered due to for instance sample leakage SET ROTOR The rotor is not installed Set the rotor Wait up to 20 min If the alarm is not cleared Thermo Scientific e Close the door completely and press the VACUUM or START button e Wipe off the moisture from inside the rotor chamber e Clean the door packing then apply a thin coat of vacuum grease Refer to section Chamber Door Seal e Check if the sample is leaking from the rotor and or tubes If so the tubes may be overfilled or may be cracked or broken In these cases reduce the amount of sample in the tubes or change the tubes e Install the rotor e Ifa
56. e procedure for performing a programmed operation that is how to call a stored set of run parameters and run this centrifuge accordingly a If you know the program name you need Step Touch Panel Operation Screen Display and Notes Turn on the POWER switch of the e Touchscreen appears centrifuge e The door unlocks Install a rotor e Install the rotor securely on the shaft The rotor does not have to be screwed into the shaft e Always use a quick setting rotor see section Applicable Rotors In the Run screen press the PROGRAM button i rpm g TIME hh mm Rema A PROGRAM button 1 apt S15 04T LAROGAAM e The PROGRAM screen appears 2 24 MX Plus Series Thermo Scientific Operation 2 Press the row of the program name for which you want to perform a program PROGRAM and make sure that the pressed row Ba E Parameter d 122456 70S 00RERTYUIOPASDFGHHIKe 2000r pa 3 i SO00r pa ad i STEP Boar pm turns blue Then press the Run button sev 1 30 J ser 20 0 ROTOR S150aT PROGRAM DNA STEP STEP 1 3 In this case this program contains Program name three steps In the Run screen the run parameters for the first step in this program are displayed e The program name is displayed on the PROGRAM button indicator e f the STEP 1 3 is displayed on the PROGRAM button indicator this means that the program you have just called stores multiple run parameters step
57. e rotor compartment has moisture or frost on It it takes a long time to reach medium or high vacuum level In such a case wipe it off with a clean dry cloth or sponge e f the sample is sensitive to temperature increases do not press the START button until the chamber is at a high vacuum level Press the START button e The START button blinks and the rotor starts rotating e The timer starts When an actual run timer is set the timer begins operating after the speed reaches the set speed e When the speed reaches the set speed the START button lights up e The ultracentrifuge waits at 5 000 rpm until medium vacuum level is reached The specified centrifugation time elapses e The STOP button blinks and the rotor starts decelerating time out or you press the STOP button 7 The rotor stops e The STOP button lights up e The stop signal sounds to indicate that the rotor has stopped Press the VACUUM button e The vacuum pump stops the air leak valve activates and the rotor chamber reaches atmospheric pressure cua tll e The door unlocks and can be opened and closed Remove the rotor e Make sure the rotor has stopped completely before removing It 2 12 MX Plus Series Thermo Scientific Operation 2 Note If the rotor chamber is evacuated insufficiently before starting operation or the ambient temperature is low 10 C or below the time waiting for a vacuum at 5 000rpm might become long Also during acceleration
58. e starting from an intermediate step You cannot run the centrifuge starting from an intermediate step in a memory unit that contains multiple steps Store register run parameters for the intermediate step and later steps in another memory unit Then call the memory unit and run it 3 What if a SPEED alarm goes on If a step stores a speed exceeding the maximum allowable speed of your rotor the system will detect it in the STEP1 run and display the SPEED alarm Double check the speed of all steps and correct any wrong ones 4 Stopping the centrifuge in operation 5 Press STOP button The rotor stops and the system does not move to the next step Selecting the Rotor This ultracentrifuge stores the maximum and average radii of each rotor in the internal memory Setting a speed causes this centrifuge to automatically calculate and display the RCF relative centrifugal force value while setting an RCF value causes the centrifuge to automatically calculate and display the speed You can control the rotor data such as total operation hours and number of runs by registering your rotors in the centrifuge Thermo Scientific MX Plus Series 2 37 2 Operation 2 38 1 Procedure for selecting the rotor Touch Panel Operation Screen Displays and Notes Check that the desired rotor is displayed in the ROTOR indicator of the RUN SCREEN When the desired rotor is not displayed press the ROTOR indicator Check whether the desired rotor is
59. ears e The deletion confirmation box Is displayed Thermo Scientific Operation 2 Press the Yes button on the deletion confirmation box PROGRAM E The program is deleted NO Program name Parameter 1 Nun gogor pm 2 1239456789 O00WERTYUIOPASOFGHHJK2 5000rpm s pee eo wae he eee ee ee Res 5000r pm Step mode Operation This ultracentrifuge incorporates a step mode operation feature which stores two or more run conditions in one program memory area and switches between different values of speed running time temperature and other parameters while in operation This centrifuge can store up to nine steps This section explains how to make settings by citing some examples 1 How to activate a step mode operation Typical settings Shown below is the example of a three step run and how to activate a step mode operation Step 1 Step 2 Step 3 150 000 rom 120 000 rom 100 000 rom Run time 1 h 30 min 30 min Temas me wc me Acceleration mode Deceleraionmode 9 9 Step 1 150 000 120 000 e Sse Jae 100 000 Bae 22222 Speed rom eee AT WY 11h30min 1h 30 min Run time Figure 2 9 A typical Step mode Run Thermo Scientific MX Plus Series 2 29 2 Operation Step Touch Panel Operation Screen Displays and Notes 2 30 In the Run screen press the PROGRAM button Press the row of the program No for which you want to create or change a program and make sure that the p
60. eleration Rate In order to meet various experimental protocols the acceleration and deceleration rates can be adjusted The figure and table below show the relationship between ACCEL DECEL code numbers selected and resulting approximate acceleration deceleration times Code Acceleration time Deceleration time No minutes minutes a ACCEL DECEL code numbers ee TE e Minimum time Minimum time 9 8 7 No Brake 6 15 5 2 4 2 5 E 3 3 E 2 3 5 ZS 1 4 aa ti if A A S F Coasting 500 i deceleration 0 The minimum time is the one that occurs when the rotor is being accelerated or decelerated with the maximum torque of the driving motor This time varies with the type of rotor in use Note The times for acceleration and deceleration may be longer than the values listed above depending upon the type of rotor in use Typical examples of application of acceleration and deceleration rates Type of Centrifugation Characteristic of Separation Density gradient centrifugation The sample and gradient in tubes reorient during using a vertical rotor acceleration and deceleration Therefore the sample and gradient can become mixed especially in wide tubes if you use rapid acceleration or deceleration DNA separation by CsCl isopycnic You can operate at maximum acceleration separation self forming gradients because the density gradient is not formed during the run As for the deceleration it is better to
61. entific MX Plus Series i Contents Chapter 4 Chapter 5 Chapter 6 Chapter 7 Appendix A Appendix B Appendix C MX Plus Series PLOW TSS NO OCU ciseus EE 4 1 lege tee 4 3 User corrected Problem viciniscibiadeciseatsaseereweddehensasisagseatalbesediebedentoblasdaealen e HE eher 4 5 JC E d EEN 5 1 SHEET 6 1 SUMED EIS E 7 1 Chemical Compatibility Chart 00 0 0 sesesessessseeeseseseseeenesaeseseeeseeaeaesesesenenanseseens A 1 WARE Hee B 1 EE EEN B 2 Contact Informatio M ee C 1 Thermo Scientific o p Preface General Description The MX Plus Series is designed to separate liquid suspended materials having different densities and particle size The MX Plus Series is a product that pursues user friendliness and reliability based on our many years of experience in developing centrifuges This centrifuge offers many features that we are confident of satisfying your requirements These features include the following 1 The maximum speed is 150 000 rpm 1 048 684 x g MX 150 2 Small and compact floor type 3 The touchscreen with easy to see color liquid crystal screen is incorporated 4 The displayed language can be switched over between Japanese and English 5 Various alarm indications can notify users of the causes and necessary actions of the troubles It can realize troubleshooting easier and quicker 6 The rotor can be set by simply mounting it on the drive shaft in the rotor chamber quick setting type 7
62. exclusively for the MX 150 Micro Ultracentri fuge Note When your centrifuge is the MX 120 Micro Ultracentrifuge you can not proceed to the ss USB LAN communication setting screen even if you press the USB LAN Communication Icon USB LAN Communication USB MEMORY The operation history of the centrifuge can be output in CSV format to a USB flash drive on the market CSV format This is a versatile file format that is compatible with spreadsheet softwares such as EXCEL USB host side RUN Screen NEMU 11 Dec 2008 9 49 USB MEMORY x Press the DATA EXPORT para The driving history data button e export s transmit Writing in the USB flash drive is automatically started Insert the USB flash drive on the market into the USB for external connection host side at the right side of the centrifuge Figure 2 27 USB LAN Communication Setting Screen Note 1 Be sure to back up your data before using the USB flash drive Thermo Fisher Scientific will not be liable for any data loss 2 This centrifuge cannot use the USB flash drive with security function 3 When powered on with the USB flash drive the centrifuge may not recognize the USB flash drive In such a case pull out the USB flash drive from the centrifuge and then insert it again into the centrifuge LAN Communication If your centrifuge has a LAN c
63. g time open the door and take out the rotor as described below WARNING Before following this procedure listen carefully for sound from within the rotor chamber to make sure that the rotor is not rotating Never unlock the chamber door while the rotor is rotating The rotor chamber is in a vacuum with low air resistance so the rotor may therefore go on rotating for approximately 120 minutes before stopping Allow enough time before opening the door 1 Make sure that the rotor has stopped 2 Unplug the power cord of the centrifuge from the wall outlet 3 Use a Hex key to remove the four screws that fasten both sides of the front cover and remove the front cover while pulling it towards you Top deck Front cover Front cover fastening screw Front cover hook Figure 2 28 Removing the Front Cover 4 Remove the vacuum release knob at the left of the vacuum chamber by turning its tip counterclockwise to allow air to enter the rotor chamber After the rotor chamber reaches atmospheric pressure do not forget to return the vacuum release knob to its previous position 2 70 MX Plus Series Thermo Scientific Operation 2 5 Pull the release wire of the door lock in the left side of the vacuum chamber toward you and open the door After opening the door make sure that the rotor is not rotating If it is rotating close the door immediately WARNING Never touch the rotor while it is rotating Vacuum cham
64. ge dampened with a diluted solution of neutral detergent If any solution that is toxic radioactive or pathogenic is spilled inside or outside the centrifuge take necessary action according to your proper laboratory procedures and methods Chamber Door Seal Thermo Scientific If the door seal O ring is dusty or scratched a high vacuum level might not be possible Always keep the door seal O ring clean If the ultracentrifuge is used frequently every three to four months ordinarily once a year take out the door seal O ring and wipe it with a clean soft cloth and then put a thin coat of vacuum grease on it If the door seal O ring is damaged replace it Wipe the groove for the door seal O ring with a clean soft cloth dampened with alcohol or a similar solvent MX Plus Series 3 3 Removing the Door Seal O Ring 1 While the door is open turn off the POWER switch and unplug the power cord from the wall outlet 2 Open the door completely Then hold the handle of the door and pull up the door Handle Door seal O ring Figure 3 1 Removing the Door Seal O Ring 3 Use a tool such as a toothpick to remove the door seal O ring from the groove At this time be careful not to damage the door seal O ring or groove 4 Clean the door seal O ring and groove If the door seal O ring is damaged replace it The part code of the replacement door seal O ring is 84520135 Note that a replacement is not provided with the accessory
65. ging the ultracentrifuge into a power source WARNING Your ultracentrifuge must be grounded properly An emergency switch circuit breaker should be installed that turns off the main power supply in the event of malfunctioning The desirable installation location of the emergency switch is outside the room or near the exit Do not position an object so that it is difficult to disconnect the power cord from the outlet If you do so you cannot disconnect the power cord from the outlet when you observe some abnormality Your ultracentrifuge can operate on one of the following six power voltages 110 or 120 Vac 50 60 Hz 15 A 208 220 230 or 240 Vac 50 60 Hz 8 A The voltage requirement for your ultracentrifuge is mentioned on the rectangular marking plate rating plate affixed near the power cord connector which is visible in the rear panel of the ultracentrifuge Be sure to read the marking plate before plugging the ultracentrifuge If the voltage requirement does not match the voltage of the available power source quit plugging and call your Thermo Fisher Scientific representative 3 Leveling 1 While referring to figure 5 2 turn the level adjusters with a spanner until the casters are to 5 to 10 mm off the floor MX Plus Series Thermo Scientific Ultracentrifuge About 5 to 10 mm Level adjuster Caster Figure 5 2 Leveling Adjustment 2 Turn on the power switch on the ultracentrifuge open the chamber do
66. he POWER switch on the device unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock WARNING 1 If the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood samples that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods 2 If there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales service representative Note that Thermo Fisher Scientific cannot repair the centrifuge rotor or the accessory unless sterilization or decontamination is completed 3 It is your responsibility to sterilize and or decontaminate the centrifuge rotor or parts properly before returning them to a Thermo Fisher Scientific authorized sales service representative In such cases copy the decontamination sheet at the end of this manual and fill out the copied sheet then attach it to the item to be returned Thermo Fisher Scientific may ask you about the treatment for the centrifuge rotor or the part if the decontamination is checked and judged as insuffi
67. he last character in the user name box e Press the Enter button to turn the screen to the PIN registration screen e Press the x button to return to the user administration screen Thermo Scientific MX Plus Series 2 57 2 Operation 2 Register the user name and press the Enter button User name E Thermo Scientific EI or PRO BS eee PS pae lear Enter button RUN screen Nenu e The PIN registration screen Is displayed 3 Enter the desired PIN number 4 digit in the e The masked PIN PIN box and press the Enter button number is displayed a aT e The PIN confirmation PIN confirm screen is displayed 2 58 MX Plus Series Thermo Scientific Operation 2 4 Enter the same PIN number 4 digit as the ake one entered in the above step 3 in the PIN PIN confirm field confirm box Press the Enter button A MUN screen Ment PIN confirm e The masked PIN number is displayed Difference of access level Admin The administrator can do every operation of this centrifuge Enter button User The user cannot use the Admin functions when the user lockout function is enabled RUN screen Menu e The access level PIN confirm selection box Is splayed Access level selection box 5 To place the registered user on the same access level with the administrator Dress Run screen Menu e The screen returns to ini j i User Management Administrator in the access level selection sen GENEE the
68. ilization or decontamination is completed CAUTION Do not perform any operation not specified in this manual If any problem is found on your centrifuge contact a Thermo Fisher Scientific authorized sales service representative This centrifuge does not require complicated maintenance and inspection To ensure safe and trouble free use for a long time follow the instructions below MX Plus Series CAUTION Using a cleaning or sterilization method other than the ones recommended in this instruction manual might cause corrosion or deterioration of the centrifuge Refer to the chemical resistance chart provided with the rotor or contact Thermo Fisher Scientific CAUTION For sterilization of the surface of the centrifuge and the rotor chamber wipe them with a cloth dampened with 70 ethanol Using the method other than the above method might cause corrosion or deterioration of the centrifuge Refer to the chemical resistance chart provided with the rotor or contact Thermo Fisher Scientific While we recommend that 70 ethanol is used for sterilization no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use CAUTION Disconnect the power cord from the outlet before cleaning or sterilizing the centrifuge Thermo Scientific For information on the maintenance of rotors and tubes see the rotor instruction m
69. inutes before pressing the CE button This 120 minutes is the time which passed after a power failure occurs 4 4 MX Plus Series Thermo Scientific User corrected Problem Some problems are not identified and reported by the self diagnostic capability of the ultracentrifuge To correct these problems take the action s described in the table below Symptom Ultracentrifuge will not Start up when Its power Is on Rotor does not cool or rotor temperature Is rising You press the START button but the rotor will not turn The backlight level of the screen is too dark or too bright You can not open the door because the STOP button continues to blink Thermo Scientific 1 Building power circuit breaker is open 2 The power cord is unplugged from the outlet Rotor was accelerated at low or intermediate vacuum level High vacuum level cannot be reached If the SET ROTOR alarm message appears the overspeed detector may issue a signal that prevents the rotor from rotating even though you pressed the CE button This is not a fault The brightness of the screen is not adjusted You pressed the START button and then you pressed the STOP button before the rotor started to rotate Possible Cause Action e Close the circuit breaker e Plug the cord into the outlet e Start accelerating the rotor when the rotor chamber is at high vacuum level after the vacuum indicator is displayed as
70. is micro ultracentrifuge The screw type rotors are inapplicable Automatic securing rotors are not applicable in older models RC M100 RC M120 and RC M120EX Centrifuges 2 The rotor must be gently placed on the drive spindle to avoid damaging the drive shaft 3 Use only rotors approved for use in the Sorvall MX Plus Series see list of approved rotors in section 7 Specifications Rotor set handle Cap blue without a screw hole Screw cap with a screw hole Cover black Cover blue Quick setting type Screw type for older models 1 Applicable rotor 2 Inapplicable Table 2 1 List of approved Rotors PN motor eieiei Volume ml Max Speed Max RCF k Rin Pave Rmax Tube places x vol ame nominal rpm x g Factor mm mm mm Angle Fixed Angle Rotors 45582 150 AT 8 x 2 0 mL 16 mL 150 000 899 744 6 1 20 8 28 3 35 8 30 45978 5140 AT 10 x 2 0 mL 20 mL 140 000 1 048 684 50 32 6 40 3 47 9 35 45584 120 AT3 14x 0 5 mL 7 mL 120 000 649 826 78 259 33 2 40 4 30 Thermo Scientific MX Plus Series 2 5 PN Rotor Capacity pices ment an e K be Se Bowe 45583 120 AT2 10 x 2 0 mL 20 mL 120 000 649 826 78 259 33 2 40 4 30 45539 S110 8x50mL Ant 110 000 690 652 15 245 378 511 30 45588 mai 8x5 0mL Ant 100 000 603 1180 18 26 9 mp 540 30 45586 mai 6x35mL nt 100 000 ue 16 258 371 484 30 45585 mai 20x0 2mL Ant 100 000 435 630 70 296 343 390 30 45589 mum 30x0 5mL on mmm 357 440 14 355 op mn 30 45590 mon 8x8
71. it button SPEED Ui DKG Keypad appears Using the on screen keypad enter the run parameters for STEP 1 see section Preparing Tubes Bottles and Rotor If you want to enter an acceleration and deceleration rate press the ACCEL DECEL button Run parameters for ee a OK button SPEED 150 000 rom TIME 2hr 30min 3 b The PROGRAM entry screen TE MP 20 C E TEP AD displays the run parameters ACCEL rs entered for STEP 1 b After entering the run parameters press the OK button If you want to change the run parameters press the STEP 1 row and make sure that it turns blue l Then press the Edit button Run button Edit button e To enter the run parameters in multiple steps in a step mode operation continue the operation from the above see section Step mode Operation e You can not skip a STEP If you enter nothing in STEP 1 you can not enter anything in STEP 2 In the PROGRAM screen press the Run button EEDEERERN a The Run screen appears TIME bb mm TEMP Remain d Program name Note 1 If you make and store changes in a column that already stores run parameters the previous parameters are replaced by the new parameters 2 You cannot store a run parameter while in running while the rotor is rotating Always perform this function while not in running Thermo Scientific MX Plus Series 2 23 2 Operation 2 How to perform a programmed operation This section describes th
72. le or in part as the result of i normal wear and tear ii accident disaster or event of force majeure iii misuse fault or negligence of or by or on behalf of the Customer iv use of the Goods in a manner for which they were not designed v causes external to the Goods such as but not limited to power failure or electrical power surges or vi use of the Goods in combination with equipment or software not supplied by Thermo Fisher Scientific If Thermo Fisher Scientific determines that Goods for which the Customer has requested warranty services are not covered by the warranty hereunder the Customer shall pay or reimburse Thermo Fisher Scientific for all costs of investigating and responding to such request at Thermo Fisher Scientific then prevailing time and material rates If Thermo Fisher Scientific provides repair services or replacement parts that are not covered by the warranty provided in this Clause the Customer shall pay Thermo Fisher Scientific therefore at Thermo Fisher Scientific then prevailing time and materials rates Any installation maintenance repair service relocation or alteration to or of or other tampering with the Goods performed by any person or entity other than Thermo Fisher Scientific without Thermo Fisher Scientific prior written approval or any use of replacement parts not supplied by Thermo Fisher Scientific shall immediately void and cancel all warranties with respect to the affected Goods Terms may va
73. les that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods 2 If there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales service representative Note that Thermo Fisher Scientific cannot repair the centrifuge rotor or the accessory unless sterilization or decontamination is completed 3 It is your responsibility to sterilize and or decontaminate the centrifuge rotor or parts properly before returning them to a Thermo Fisher Scientific authorized sales service representative In such cases copy the decontamination sheet at the end of this manual and fill out the copied sheet then attach it to the item to be returned Thermo Fisher Scientific may ask you about the treatment for the centrifuge rotor or the part if the decontamination is checked and judged as insufficient by Thermo Fisher Scientific It is your responsibility to bear the cost of sterilization or decontamination If you have any question please send e mail Note that Thermo Fisher Scientific cannot repair or inspect the centrifuge the rotor or the accessory unless ster
74. leting a program To delete a program delete all steps in that program Note You cannot delete a program while in running while the rotor is rotating Always perform this function while not in running Step Touch Panel Operation Screen Displays and Notes 1 In the Run screen press the emm PROGRAM button Ee set 150000 SES UNE GEN PROGRAM button SPEED npm SET rpm SPEED ACCEL 9 RGF DECEL7 qe TONE ei The PROGRAM screen HO Program name Paraneter appears TEEN 1S0000f pm Press the row of the program name for which you want to delete a program and make sure that the pressed row turns blue Weeer Then press the Delete button rated naw Sou a delete this progran PROGRAM e The deletion confirmation Wi rogram name Parameter box IS displayed 5000rpm 2 36 MX Plus Series Thermo Scientific Operation 2 Press the Yes button on the deletion confirmation box PROGRAM x Wi Program name Parameter 1 Kuna S000F pm 2 123456780 008ERT YU OPASOFGHHJIK2 SO000r pm e The program is deleted d suai rabardisa 2000r pm 3 Other procedures 1 Making changes to the run parameters Call the memory unit you want to make changes to and make the changes Alternatively delete the memory unit then store a new set of run parameters To make a change that will result in fewer steps delete the memory unit and then enter a new set of run conditions and store them 2 Running the centrifug
75. llow for centrifugation time and set the centrifugation start time to a time later than the current time e You cannot set the centrifugation start time to a date more than 20 days in the future 2 Press the Enter button e The START confirmation box appears 04Dec 6 00 08Dec 8 00 Thermo Scientific MX Plus Series 2 49 2 Operation 3 Press the OK button and the START button The RIC run does not get activated unless you 08 Dec 2008 11 50 press the START button 7 SPEED rpm CD TIME _ nh mm fama i n Di 0 00 SET 150000 SET 2 00 ROTOR ue d START 09 Dec 6 00 eT STOP 09 Dec 8 00 PROGRAM e The START time and STOP time for centrifugation are displayed on the RIC field by pressing the START button This centrifuge will turn the rotor automatically at the specified time e Pressing the START button enters the system into a condition causing the centrifuge to wait until the set time comes This centrifuge will turn the rotor automatically at the specified time and run It for the specified time e Once the RIC setting is made you cannot change the run time centrifugation time If you wish to make a change to the run time cancel RIC first If you want to cancel the RIC press the RTC button on the Run screen and press the Cancel button on the SET RIC screen e f you cancel RTC when the user lockout function see section Administrator Admin Functions 2 on this function is enabled the foll
76. me to a complete stop This alarm code appears when the press the SPEED column HOLD 9 0 1 HOLD and Enter detector of this ultracentrifuge could not Dutton in order when the Run screen is displayed Then press identify which status status 1 or status the VACUUM button This ultracentrifuge accepts the VACUUM 2 occurred button Status 1 The rotor stopped normally First open the door a little to confirm that rotor has come to a Status 2 Although the rotor continued to complete stop rotate the speed of the rotor was not Then open the door properly detected due to a failure of the speed detector If this alarm code appears this ultracentrifuge does not accept the CE or VACUUM button for 120 minutes to ensure safety The same thing can be said of E13 Refer also to section Emergency Recovery from Power Failure E11 E64 Refer to NOTE e Calla service representative If any alarm message between E11 and E64 appears this ultracentrifuge requires maintenance by a POWER FAILURE kkk rom Door is closed Refer to manual for further detail service representative When ordering a repair inform us of the alarm code you have received Note E13 is an alarm code that indicates a failure of the speed detector If this alarm code appears this machine does not accept the CE or VACUUM button for 120 minutes to ensure safety These 120 minutes are required for the rotor to stop Wait at least 120 m
77. min Functions 5 Rotor Management you can select it in the PROGRAM Rotor Management screen b The PROGRAM ROTOR Rotor Catalog Name dv S150 AT 150000 29744 711250 Be 1 00 ip 120 AT3 120000 645826 533211 fsx 0 50 si20 vT 120000 500237 410182 Bx 2 00 wen kend c The PROGRAM screen re appears If you selected the S150AT these characters are displayed in the Rotor field Program name User namo Thermo Scientific Paraneter SPEED ACFimax MX Plus Series 2 21 2 Operation Step Touch Panel Operation Screen Display and Notes select run parameters as follows PROGRAM Program nane To enter a SPEED value If you want to enter a SPEED value as mole Thermo Scientific a parameter press the SPEED area and omg make sure that it turns blue Then Edit button Parameter SPEED ROCF avg SPEED area d EDIT button EM e The PROGRAM entry screen STEP SPEED TIE appears S J Le ed d KS C LS C DS ES To enter a RCF value If you want to enter a RCF value as a PROGRAM parameter press the RCFmax or ve RCFavg area and make sure that it User name turns blue Thermo Scientific Then press the Edit button RCFmax area RCFavg area Edit button 2 22 MX Plus Series Thermo Scientific Operation 2 Step Touch Panel Operation Screen Display and Notes 7 Enter run parameters as follows a Make sure that the STEP 1 row is a The on screen blue and then press the Ed
78. mode feature For details see section Step mode Operation Run the centrifuge under normal e Run this centrifuge according to section Operational Procedure operation without making changes to e If you make changes to the run parameters such as SPEED and TIME after calling the run parameters a program the program you have just called is canceled You must call it again to use it Thermo Scientific MX Plus Series 2 25 2 Operation b If you do not know the program name you need Touch Panel Operation Screen Displays and Notes Turn on the POWER switch of this e Touchscreen appears device e The door unlocks 2 Install a rotor e Install the rotor securely on the shaft The rotor does not have to be screwed into the shaft 1 e Always use a quick setting rotor see section Applicable Rotors 3 In the Run screen press the PROGRAM button BN a i PROGRAM button ASCE S i a SS e The PROGRAM screen HO Program nane Paraneter appears 2 123456 7SS00WEATYUI OPASOPGHHJES S000r pe DOOD pm 2 26 MX Plus Series Thermo Scientific 4 Press the row of the program name whose contents you want to check and make sure that the pressed row turns blue Then press the Select button Check the user name and rotor name in the Program screen and decide whether this selected program is the program that you need or not If you think that the selected program is the program you need press the Edit
79. n Functions 1 User Management before attempting to log in Thermo Scientific MX Plus Series 2 43 2 Operation Features of the MENU Screen The Menu screen appears by pressing the MENU screen tab on the Touchscreen These features are designed to allow you to use the MX Plus Series with additional handy options see figure 2 10 MENU tab wf History mw 2 Se N be PULSE Customize Manager Operation CAdmin gt Figure 2 10 MENU Screen The functions of each icon on the MENU screen are described in the table below The information about the run parameters of a previous normal operation can be stored automatically in the centrifuge and you can load it for a new run see section Displaying the Run History and Loading the Information About Run Parameters Rotor Catalog You can view the applicable rotor names and their specifications see section Rotor CH Catalog spin Down While you press the PULSE button on the Run screen and are holding down it the Operation l e centrifuge accelerates up to the set speed at the maximum acceleration rate When you release this button it starts deceleration This feature is useful to remove the adhered samples on the interior walls of the tubes see section Spin down PULSE mee Operation Operation RIC real time Run starts or completes at a required date and time see section RTC Real Time control et Control Operation Operation
80. n press the Enter button oam 712000 8x 1 00 The pressed row turns from white to blue nen 15 Jan 2009 14 54 CO om DIe0AT 140000 10S0000 883000 10x 1 00 Ech S140HT 140000 72000 GOOD Bx 2 00 jp S120AT2 120000 660000 530000 10x 1 00 LA ip S120aTa 120000 650000 534000 14x 0 50 Thermo Scientific MX Plus Series 2 39 2 Operation 3 When RCFmax or RCFavg is displayed in the area press the SPEED RUF button to show SPEED in the SPEED area Press the SPEED area and enter the SPEED value you want to convert into the RCF value with the on screen keypad e g Rotor S150AT SPEED 100 000 rpm Press CTT HI and Cl press the Enter button to enter the setting 4 Press the button to turn the SPEED area to the RCFmax area or the RCFavg area 2 40 MX Plus Series e See section Setting Run Conditions SPEED area set 150000 SPEED r ROTOR SET rpm SISOAT SPEED ACCEL 9 PROGRAM ron DECEL 7 om i Jan 2009 14 52 ee Dh mm N TEMP Cc S Remain 100000 0 00 25 0 set 150000 ser 2 30 x GERS ee ee re e The RCF value of the rotor displayed in the ROTOR indicator is displayed on the lower part of the RCF area T TIME h mm Remain npm SET 1 00000 rpm SPEED ACCEL 9 RCE DECEL T N USER RCFmax area The maximum RCF value for the rotor set speed is displayed RCFavg area The average RCF value for the rotor set speed is displayed This is the RCF
81. n wn c Zn nm wn n wn Z Si a nm nm wm wm wm wm wm YM YM Co Co Cc DERIN _ ETHYLENE PROPYLENE N CI Gol WN GLASS Mm WN WN WN WN WN Mi MH MA A A ND YN WM Mm MN WN WN WN WN WN Z z AI nN WwW NA nM WwW WM Z C C a oi WM Gol oi a C NEOPRENE Z Hl c CO WM il WM Cl nm oi Gol Mm oi NORYL Mm MN WN WN ND ND WN WN WN Mm WM C TWN WN WN WN NM Mm ND DI WD WI WMD ND NDI WM WI Gol WwW C NYLON Mm MN WN WN WN WI Z Tl NI WD WD WN CT Got WN WM MN nN Z C C C oi nN Loi Gol Z PET POLYCLEAR CLEARCRIMP C C C Co Wm WM C Cc Gol Got Z AM Col Go Cl oi TC nM Gol CTC zZz oi wm Z Gol nm ol Z Go ol nm nm nm wm wm Z oi ol ol WwW ol POLYALLOMER WM nm C C Cl C wm WM C Cl oi Cou Z Z Z ol C C wm C wm oi C wm CC oi Gol oi Go Gol c POLYCARBONATE CC POLYESTER GLASS THERMOSET TMA ND MD DM YW Z C Gi CC POLYTHERMIDE CMM DM WM Hi nn Gi Clay nN WN Go POLYRTHYLENE AM Col Go Cl oi TC NM MW CTC ZI oi wm Z ol wm Z Z Go nl oi nm nm Gol mM ol oi WM ol oi Go Co POLYPROPYLENE POLYSULFONE WM MM MN MN YM Z Mi H nm nm GO WM C Cc NA WN WM WN amp POLYVINYL CHLORIDE Z Hl c CC WM WM ZER S UU S S M S S S S S S S U o S S U S r e S M S MS S S M S S S S S U S MU U U S S M S S M S MU o U S S S SS MMM S S S S S M S U S S S M S M 5 Ss D e S M S MS
82. nditions 1 Displaying the Run screen Screen for setting run conditions Thermo SCIENTIFIC SORVALL MX Series Mhicra Witracentnfige Initial screen 2 The initial screen appears 18 Jan 2008 10 11 AOTOR S15 04T _ PROGRAM 3 The Run Screen appears Run Screen Figure 2 1 Initial Screen and Run Screen Preparing Tubes Bottles and Rotor The MX Plus Series allows you to balance by eye tubes or bottles containing a sample solution and then centrifuge them Make sure that the approximate difference between meniscus levels of sample solution in tubes or bottles is within 5 mm see figure 2 2 Thermo Scientific MX Plus Series 2 3 Within 5mm For an S140AT rotor an S100AT5 rotor and an S50A rotor set the approximate meniscus level difference to within 3 mm and for an S110AT rotor and an S80AT3 rotor set it to within 4 mm Figure 2 2 Balancing Iubes Bottles containing a Sample Solution CAUTION Do not run the ultracentrifuge in an extremely imbalance condition This might cause a mechanical failure When balancing tubes or bottles by eye alarm message IMBALANCE might appear Balance tubes or bottles more accurately again if alarm message IMBALANCE appears Depending on the type of tube or rotor combined with this machine an excessively low liquid level may limit the speed or crush the tube e When sealed tubes are used fill the tubes with liquid to the full level CAUTIO
83. ng or changing This section describes the procedure for storing creating or changing a run condition If you want to store the user name in the program you have to load the user name into the centrifuge in advance see section Administrator Admin Functions 1 bal Touch Panel operation Screen Display and Notes In the Run screen press the PROGRAM button ee ees PROGRAM button SPEED r m SET mm E ACCEL 8 e RCE DECEL 7 au PROGRAM Tel e The PROGRAM screen NW Program name Paranoter appears 1 Mun sooorpm 2 12345678S900WEATYUIOPASOFGHHJK2 s00orpm S000r pm e Ir a program name and parameter are displayed in the row of a program No run parameters have already been stored for that program e f you want to create a new program press a row that does not display a program name or parameter 2 Press the row of the program No for which If vou want to store the run you want to create or change a program Ain ea pho as program and make sure that the pressed row turns pL gn oe blue Then press the Select button No 4 press the row of the 2 123456 7890QWERTYUIOPASDFGHHJEZ S000rpm program No 4 2000r pm Select button If you want to change the contents of the SS e The PROGRAM screen program change the desired item see Program nane changes below a nane Program name field User name field Rotor field Parameter SPEED 7 Thermo Scientific MX Plus Series 2 19 2 Opera
84. ommunication option LAN communication start stoppage can be specified Insert the LAN cable into the LAN port at the right side of the centrifuge LAN port option 2 68 MX Plus Series Thermo Scientific Operation 2 Connect LAN communication starts Press this button to establish the communication between himac LogManager option on your computer and your centrifuge It is possible to manage operation results of this centrifuge by using himac LogManager option If you press this button either of the below icons is displayed on the upper side of the VACUUM button on the Run screen J5 There is no problem in communication between himac LogManager option on your computer and your centrifuge There might be problems in communication between himac LogManager option on your computer and your centrifuge Follow the below instructions e Check whether the LAN cable is connected to the centrifuge properly e Check whether the himac LogManager on your computer is started e Check whether the LAN works properly Disconnect LAN communication stops Note Even if your centrifuge does not have a LAN communication option the Connect button is surrounded with a red frame as you press the Connect button In that case this icon is always displayed on the Run screen but there is no problem Emergency Recovery from Power Failure Thermo Scientific DANGER Before removing the fron
85. or and then turn off the power switch If this ultracentrifuge is still unplugged refer to section Emergency Recovery from Power Failure remove the front cover and open the chamber door 3 Place the level furnished with this ultracentrifuge on the drive shaft in the rotor chamber and turn the four level adjusters to adjust the levelness of this ultracentrifuge figure 5 3 4 After adjusting the levelness make sure that the four level adjusters are placed on the floor properly If the ultracentrifuge does not rattle when it is lightly pushed around it is installed properly Figure 5 3 Level Placement CAUTION Your ultracentrifuge must be grounded properly After installation and before any test run this ultracentrifuge always needs the internal check by a service representative 4 Moving the ultracentrifuge Before moving the ultracentrifuge disconnect the power cord turn the level adjusters with a spanner and lower the casters onto the floor Raise the level adjusters sufficiently and move this ultracentrifuge After moving this ultracentrifuge always install and level it again CAUTION Before moving the centrifuge take the rotor out of the rotor chamber Pay attention to unevenness or an inclination of the floor and move the centrifuge so that it is not fallen down Thermo Scientific MX Plus Series 5 3 ee Specifications Maximum Maximum Speed 150 150 000rpm 120 Dim Maximum Re 1 Te n 68 xg 771
86. orates a detector designed to prevent the rotor from spinning at a speed exceeding the maximum allowable speed Two independent microprocessors CPUs check the rotor for overspeed thus increasing safety further dual CPU overspeed preventive mechanism The first CPU detects overspeed and performs control and display Should the rotor be set to a speed exceeding the permissible speed this CPU detects an alarm massage of SPEED from the low speed range about 2 000 rpm and stops the rotor But this second CPU does not display an alarm message because it is not connected to the display performing CPU Should the alarm device be activated pressing START button will not run the instrument Turn off the POWER switch wait for several minutes turn the POWER switch on again then pressing START button Thermo Scientific MX Plus Series 1 7 Operation Thermo Scientific MX Plus Series 2 1 The MX Plus Series is capable of operation in more than one mode to meet a wide range of applications RER Section Normal operation a Basic Operation You can store set run conditions in memory for later use in repeated operation L7 Store gt L s Call Section Programmed Operation Programmed operation Ime More than one normal operation can be combined into a sequence of operations or step for successive C7 centrifugation Section Step mode operation Step mode Operation This feature calculates
87. owing screen appears Inputting the login user PIN or the administrator PIN is required in this screen 2 50 MX Plus Series Thermo Scientific Operation 2 Note 1 You cannot make an RTC setting in any of the following cases 1 When the Run screen is set to HOLD continuous run Set the run time centrifugation time not to HOLD but to a numerical value 2 When it is past the start time Set the start time to a time later than the current time 3 When the start time is more than 20 days after the current time Set the time to a time no more than 20 days afterwards 2 To change the run time centrifugation time after making an RTC setting cancel RTC and then set a new run time 3 To perform a combination of a programmed operation including a step mode operation with an RTC run call a program memory unit then set RTC The system calculates the total run times of all steps of the programmed operation and calculates the start time for RTC Therefore cannot call the program memory unit after setting RTC 4 To stop this ultracentrifuge in RTC operation press the STOP button The system then stops RTC and stops the rotor Customizing the Settings You can customize the settings on the Run screen and the settings on the CUSTOM X screen such as the stop signal sound volume and backlight Customize In the MENU screen press the Customize icon Then the CUSTOM screen showing the five functions i
88. pment may be impaired e The safety reminders are indicated as shown below The signal words DANGER WARNING and CAUTION are indicated together with the hazard alert symbols in this manual DANGER This note indicates an imminently hazardous situation which if not strictly observed could result in personal severe injury or possible death WARNING This note indicates a potentially hazardous situation which if not strictly observed could result in personal severe injury or possible death CAUTION This note indicates a potentially hazardous situation which if not strictly observed could result in personal injury or severe damage to the instrument This hazard alert symbol indicated together with a signal word is a reminder to emphasize important safety instructions The signal word NOTE indicates a note which has no direct bearing on personal safety e Do not perform any operation not specified in the instruction manual If any problem is found on your instrument contact a Thermo Fisher Scientific authorized sales service representative e Although the safety precautions in the instruction manual and safety instructions on your instrument have been fully considered an unexpected situation may arise Observe the instructions in the instruction manual and always be careful yourself when operating this instrument iv MX Plus Series Thermo Scientific Mechanical Safety Thermo Scientific P Preface Safety Notices WARNING
89. pressed the Enter button repeat step 1 of the previous page and then enter the correct value 2 When setting the two or more of three run conditions SPEED TIME and TEMP you do not have to press the Enter button after each setting You can store the setting by pressing the desired item 3 If the system is running in HOLD and you want to set it to shut down at a future time enter a new time setting while the instrument is in operation enter the sum of the time elapsed plus the time remaining If for example this machine has run continuously for five hours and you want to stop it one and a half hours later press the TIME area then enter 6 Bo Thermo Scientific MX Plus Series 2 9 INO s How to set speed running time temperature and other parameters Here are some examples and descriptions RPM SPEED Typical setting 150 000 rpm D gt O oO O A oO O Wei ab Q Press the SPEED TIME or TEMP area to show the on screen keypad The last entered value is displayed on each area Press the area of the desired item again if the color of the first digit is white Go to procedure 3 if the color of the first digit is blue Enter the desired numeric value with the on screen LZ SST Entered numbers are moved to the left every time a new number is entered Make a check then if you want to change other settings press the area of the desired item If you do
90. ram the program you have just called is canceled You must call it again to use it Note 1 To perform a combination of a programmed run with RTC real time control see section RTC Real Time Control Operation call a programmed memory unit then set RTC The system will then calculate the total of the running times of all steps of the programmed run and calculate the start time for RTC Therefore cannot call the program memory after setting RTC Thermo Scientific MX Plus Series 2 2 2 Operation 3 Deleting a program This section describes the procedure for deleting a program To delete a program delete all steps in that program Note You cannot delete a program while in running while the rotor is rotating Always perform this function while not in running Step Touch Panel Operation Screen Displays and Notes 1 In the Run screen Press the PROGRAM button 19 Jan 2009 10 11 T Te hh mm Remain set 150000 PROGRAM NO Program name Parameter 1 Run 5000r pm 2 123456789 0QWERTYUIOPASDFGHHJE2 5000r pm SOOO F pin 4 DWA STEP 150000r pm a e H Press the row of the program name for which you want to delete a program and make sure the pressed row turns x blue Then press the Delete button Program nane Parameter 1 Nun Sopor pm Are you sure you want to delete this program 2 28 MX Plus Series PROGRAM button e The PROGRAM screen app
91. ressed column turns blue Then press the Select button MX Plus Series enw jan 2009 10 11 Je ee ee d D rpa T TIME hemm l il TEMP Ram 0 00 25 0 set 150000 set 2 30 UU set 4 0 PROGRAM button npm pm BD ACEL 7 CAM e En Gr e The PROGRAM screen apears PROGHAM x HO Program mame Parameter 1 Hun SO000r pm 2 12345676900HERTYU 0OPASDFGHHJKZ 2000r pm e f a program name and parameter are displayed in the row of a program No run parameters have already been stored for that program e f you want to create a new program press a row that does not display a program name or parameter If you want to store the run parameters as program No 4 press the row of the program No 4 PROGRAM NW Program name Parameter 1 Run SO00r pm 2 123456 TRS 00RERTYU OPASDFGHHIKE 5000r pm a ahaa eee ante en eg we ae ed po eee eae 50007 pm Select button e The PROGRAM screen changes Program name ee User name Program name field User name field Rotor field Thermo Scientific Operation 2 3 Enter a program name as follows a In the PROGRAM screen press the a The Program name screen program name field Program name appears one step H dei b If you enter the b In the Program name screen enter ih 2q od oy Sy od 7H 4 OM OF DNA i STEP th Ae OAD RAG CL characters are e in m p m el the Program name field c Press the Enter button biad
92. ry by country Please contact your local sales office for further information MX Plus Series B 1 After sales Service Periodic inspection of the centrifuge is recommended to assure safe and efficient operation If the centrifuge fails to function normally do not attempt to repair it yourself Contact a Thermo Fisher Scientific authorized sales service representative It is requested that you return the faulty product with this Decontamination Sheet in order to repair it safely in our plant B 2 MX Plus Series Thermo Scientific Contact Information Country United States Canada Austria Belgium Germany France Italy Netherlands Nordic Baltic Countries CIS Russia Spain Portugal Switzerland UK Ireland Australia China India Japan Other Asian Countries New Zealand Latin America Other Countries o Y Telephone number 866 9 THERMO 1 866 984 3766 43 1 801 400 32 53 73 42 41 08001 536 376 49 6184 90 6940 33 2 2803 2180 39 02 95059 448 31 76 571 4440 358 9 329 10200 7 812 703 42 15 34 932 23 09 18 41 44 454 12 12 44 870 609 9203 61 39757 4300 86 21 6865 4588 86 10 8419 3588 1800 22 8374 91 22 6716 2200 81 45 453 9220 852 2885 4613 64 9 980 6700 1 866 984 3766 49 6184 90 6940 MX Plus Series C 1 Part of Thermo Fisher Scientific SCIENTIFIC
93. s displayed as shown in figure 2 12 Tab of each Run scrfen et le giffe Omm Zoom Stop Signal Volume Backlight E Bn Vacuum Waiting Figure 2 12 CUSTOM Screen Thermo Scientific MX Plus Series 2 51 2 Operation The functions of each icon on the CUSTOM screen are described in the table below bes eon Jose The display on the Run screen can be zoomed in see section Customizing the settings 1 Stop signal The rotor stop signal can be selected from six kinds of sound including five tunes and D electric beep see section Customizing the Settings 2 Stop Signal Volume The volume of the stop signal can be adjusted see section Customizing the settings 3 Brightness The brightness of the screen can be adjusted see section Customizing the Settings TY 4 Ae pe Backlight Vacuum The conditions of the waiting for the vacuum of the rotor acceleration state can be Standby Setting WI specified see section Customizing the Settings 5 Vacuum Waiting Press the desired icon Then the corresponding item is displayed After setting press the tab of the desired screen 1 Zoom The display on the Run screen can be zoomed in RUN SCTrEEN Menu Zoom Setting 1 Normal Displays the ordinary Run screen 2 Zoom The speed and time display is zoomed when 20 seconds have passed after reaching the set speed Press either the NORMAL or ZOOM button and make sure that the
94. screen Menu User R Rotor Japanese Management Lockout Rotor Date Time 1D Contact Management USB LAN Communication h Figure 2 18 ADMIN screen The functions of each icon on the ADMIN screen are described in the table below User Management The user name can be registered and deleted see section Administrator D Admin Functions 1 User Management Thermo Scientific MX Plus Series 2 55 2 Operation User Lockout Rotor Lockout Japanese Rotor Management Actual Run Timer Date and Time display Centrifuge ID service Contact USB LAN Communication sa Rotor Lockout p AS KE Japanese Ce Rotor Management Actual Run Timer Ld Date Time II D Contact ao USB LAN Communication User login can be required to start operation see section Administrator Admin Functions 2 Run history of each rotor can be controlled see section Administrator Admin Functions 3 English and Japanese can be toggled in display see section Administrator Admin Functions 4 You can use and control the rotor data such as total operation hours and number of runs see section Administrator Admin Functions 5 Actual run timer can be selected see section Administrator Admin Functions 6 The date and the time can be set see section Administrator Admin Functions 7 Centrifuge ID can
95. selected button Is surrounded with a green frame Then press the x button or the CUSTOM tab to store the setting To return from the zoom screen to the normal screen i l during operation press anywhere except the STOP Figure 2 13 Zoom setting screen button Then the screen returns to the normal screen Press the Customize icon of the MENU screen and select the NORMAL according to the above procedure 2 52 MX Plus Series Thermo Scientific gt Stop Signal Oz L Backlight Thermo Scientific 2 Stop signal The rotor stop signal can be selected from six kinds of sound including five tunes and electric beep Press the row of the desired stop signal Then the stop signal sounds and the row of the selected stop signal turns blue Press the Enter button to store the setting To set another function on the CUSTOM screen press the x button or the CUSTOM tab 3 Volume adjustment The volume of the stop signal can be adjusted The sound volume is turned up as the green range in the volume setting indicator increases The stop signal does not sound if there is only the black range Adjust the sound volume by pressing the following buttons lt The volume is lowered by pressing this button gt The volume is turned up by pressing this button The stop signal does not sound by pressing this button The stop signal sounds at full volume by pressing this button Press the x button or the CUSTOM screen
96. setting can cause severe damage to the instrument Make sure that all the rotor buckets are of the same type e If abnormal sound or vibration occurs stop the operation immediately and contact a Thermo Fisher Scientific authorized sales service representative MX Plus Series V P Preface safety Notices CAUTION e Be sure to remove the rotor from the rotor chamber when centrifuge is not used for a long time or when the machine is moved Otherwise the drive shaft crown may be damaged e Before using a rotor be sure to read through the rotor instruction manual e Check the chemical resistance chart attached to the rotor and do not use any sample inapplicable to the tubes tube caps bottles or bottle caps etc Using such a sample could corrode or deteriorate such parts e Use the rotor tubes and bottles within their actual capacities e Mount the rotor onto the drive shaft gently and properly e Do not drop the rotor or apply excessive force to the drive shaft to avoid damage to the drive shaft e Maximum rotor speed depends on the tubes or adapters to be used Follow the instructions on the rotor instruction manual e Approximately even quantities of sample in the tubes are sufficient for balancing and extremely different sample quantities must be avoided Refer to section Preparing Tubes Bottles and Rotor for the sample balancing e Clean the inside of the drive hole crown hole of the rotor and the surface of the drive sh
97. starts temperature control Press this button to stop rotor rotation Displays the program No if program operation has been selected Press this button to specify program settings see section Programmed Operation Displays the start time or finish time for centrifugation Press this button to set a start time or finish time see section RTC Real Time Control Operation Displays the user name see section Administrator Admin Functions Use the on screen keypad to enter numeric values for run parameters When entering a time Switches from hours to minutes Hold When entering the operation time Sets continuous running FREE When entering deceleration conditions Sets free coasting CE Press this to cancel input for example if you enter the wrong number or the wrong value for a run parameter Enter Press this to save the entered setting X Press this to close the keypad display MX Plus Series 1 5 External Connection This Function is exclusively for the MX 150 Micro Ultracentrifuge 10 USB host side Use the USB connection to output the operation history of the centrifuge to a 3 USB flash drive Rotor Chamber The structure of the rotor chamber vacuum chamber is shown in figure 1 4 Cooling Bowl Rotor Chamber Cools the rotor Chamber Door Seal a See Drive Shaft crown oS a TNN T EEE E Protector Protection steel tube _ Temperature Sensor Measures
98. status in the upper part and the specified setting in the lower part The button for the acceleration ACCEL and the deceleration DECEL displays the specified setting 19 Jan 2009 10 11 Lf SPEED rpm TIME hh mm Le om Ve emain Current status set 150000 SET 2 30 Specified setting SPEED rpm ROTOR SET rpm S150AT PROGRAM _ ne SPEED ACCEL 9 are DECEL 7 Figure 2 3 Run Screen Thermo Scientific MX Plus Series 2 7 Display and Operations when Entering the Run Parameters The on screen keypad appears by pressing the SPEED area TIME area or ACCEL DECEL button 1 Press the area of the desired item to turn the first digit blue rpm 2 Enter the desired numeric value with the on screen keypad e g SPEED 150 000rpom Press the 1 5 0 On screen keypad f SPEED rpm d 150000 SET 100000 3 If you do not want to change any other setting press the ENTER button on the on screen keypad If you want to change other settings press the area of gm SPEED ae rpm the desired item and the pressed area will become the input wait state The new setting is displayed in 0 the lower part of the pressed area set 150000 Figure 2 4 Displaying the New Setting 2 8 MX Plus Series Thermo Scientific The next page describes how to set run condition by citing some examples Note 1 If you enter the wrong value press the CE button and then enter the correct value If you have
99. t cover for troubleshooting always turn off the POWER switch on the device unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock WARNING 1 Never open the door during rotation 2 Never touch the rotor during rotation CAUTION Never conduct operations in a manner other than as described in this operation manual Contact with your nearest service representative if any problem arises 1 Rotation of the rotor The rotor coasts to a stop free coasting However if the rotor did not go below 300 rpm or lower when a power failure occurred after power returns the rotor will automatically accelerate again and return to the set speed before the power failure If the speed did go below 300 rpm after power returns the rotor will decelerate and stop If the rotor is stopping when the power returns buttons such as the VACUUM button will have no effect on the centrifuge see section Alarm Indicators POWER FAILURE rpm 2 Touchscreen display MX Plus Series 2 69 2 Operation All displays are off during a power failure After power returns the instrument starts control again at the settings specified before the power failure The settings have battery backed back ups The device then displays an alarm message indicating that it has suffered a power failure 3 Removing the rotor from the ultracentrifuge If a power failure lasts or is likely to last a lon
100. tab to store the setting 4 Backlight setting The brightness of the screen can be adjusted The backlight brightens up as the green range In the backlight setting indicator increases The backlight level is darkest if there is only the black range Press the following buttons to adjust the brightness of the screen Press the x button or the CUSTOM screen tab to store the setting lt The brightness is decreased by pressing this button gt The brightness Is increased by pressing this button Operation 2 CL A3 screen enw The beeper sounds two beepes Figure 2 14 Stop signal setting screen ALM screen Menu Volume Setting Figure 2 15 Volume setting screen ALM SCEE Menu Backlight Setting Figure 2 16 Backlight setting screen MX Plus Series 2 53 2 Operation 5 Vacuum standby setting The conditions of the waiting for the vacuum of the rotor Aun screen Menu acceleration state can be specified WI Vacuum Standby Setting Vacuum Waiting 1 Normal When the vacuum level returns from medium to low during acceleration at 5 000 rpm or higher speed the viet centrifuge keeps on running at that speed until the Boras Reie vacuum level becomes medium again 2 No Waiting Acceleration The centrifuge keeps on accelerating when It Is accelerating at 5 000 rom or higher speed even after the vacuum level returns from medium to low Figure 2 17 Vacuum standby setting screen Press either
101. the following In a high vacuum e Inspect the door seal O ring see section Chamber Door Seal e Turn off the POWER switch wait several minutes turn on the POWER switch again and press the START button If this procedure still does not activate this ultracentrifuge call a service representative e Make the adjustment while referring to the clause Backlight setting in section Customizing the Settings e Because the detector of this ultracentrifuge could not identify which status status 1 or status 2 occurred the safety devices function The centrifuge maintains deceleration status at that time Status 1 The rotor stops normally Status 2 The speed detector is faulty Open the door of the centrifuge after the safety devices are released after from 10 seconds to approximately four minutes MX Plus Series 4 5 ee Installation This chapter describes the electrical power requirements location and environment that you must provide for your ultracentrifuge prior to its installation by an authorized Thermo Fisher Scientific representative DANGER Before removing the cover or other components always turn off the POWER switch on the device unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock Note The installation and leveling of your ultracentrifuge must be done by an authorized Thermo Fisher Scientific representative If they
102. tion 3 Enter the program name as follows a In the PROGRAM screen press the a The Program name program name field e y screen appears j EE b If you enter DNA b In the Program name screen enter the these characters are program name AHH displayed in the program name field c Press the Enter button c The PROGRAM screen Progran nane re appears User name Rotor 4 Select a user name as follows D a The PROGRAM User name Access level Last run Screen appears OS Dee 2008 10 18 a In the PROGRAM screen press the User name field b Press the row of the user name you want to select Then press the Enter button b The PROGRAM screen remm re appears If you selected ea te Thermo Scientific these characters are displayed in the User name field Parameter 2 20 MX Plus Series Thermo Scientific Select a rotor as follows a ln the PROGRAM screen press the Rotor field b In the PROGRAM Rotor Management screen press the Rotor Catalog button c In the PROGRAM ROTOR CATALOG screen press the row of the rotor you want to select Then press the Enter button Thermo Scientific Operation 2 Step Touch Panel Operation Screen Display and Notes a The PROGRAM Rotor PROGRAM Eg notor manaeun Management screen appears Name SIN Hours Runs Ho ragistered roter Last run e f you registered your rotor in advance see section Administrator Ad
103. ton Run parameters for STEP 1 SPEED 150 000 rpm TIME br 20mm TEMP 20 SS b The PROGRAM entry screen ACCEL 9 SCENE displays the run parameters entered DECEL 9 SE EEN E for STEP 1 After entering the run parameters press the OK button If you want to change run parameters press the row of the desired STEP and make sure that it turns blue Then press the Edit button b Use the on screen keypad to enter Run button Edit button the run parameters for STEP 2 ina e You can not skip a STEP If you enter nothing in STEP 1 you similar way cannot enter anything in STEP 2 Run parameters for STEP 2 rom map O c The PROGRAM entry screen SPEED 120 000 rpm displays the run parameters entered TIME 1hr eae a ieee for STEP 2 TEMP 20 ACCEL 9 DECEL 9 c Use the on screen keypad to enter the run parameters for STEP 2 ina similar way ec d The PROGRAM entry screen ee 3 O Ine displays the run parameters entered 100 000 rpm for STEP 3 TIME 30min TEMP 20 ACCEL 9 DECEL 9 2 34 MX Plus Series Thermo Scientific Operation 2 In the PROGRAM screen press the Run button PROGRAM DNA STEP En The Run screen appears STEP SPEED TIME EP A D 1E0000 1 30 20 0 8 8 F 1 19 Jan 2008 10 52 TEMP d Set 20 0 P P PROGRAM 5 DNA STEP Program name Run the centrifuge under normal e Run the centrifuge according to section Operational Procedure operation witho
104. ty with all relevant terms of directive for machinery 2006 42 EC Die Maschine ist auch in Ubereinstimmung mit allen einschl gigen Bestimmungen der Richtlinie 2004 1 08 EG ber elektromagnetische Vertr glichkeit The machinery is in accordance with all relevant terms of directives for electromagnetic compatibility 2004 108 EC Die Schutzziele der Niederspannungsrichtlinie 2006 95 EG werden eingehalten The protection goals of the directive for low voltage 2006 95 EC are met Angewandte harmonisierte Normen Harmonized standards used EN 61010 1 2004 EN 61010 2 020 2006 EN 61326 1 2006 CLASS A Osterode den 20 07 2012 dh dl Dr Andreas Karl Director R amp D Ort Datum Name Funktion und Unterschrift Place date name function and signature po Name i Datum ee LD kument O Revision S O O O Erstellt H Sindram 20 07 2012 50136856 _MX120 _Mx150 Doc 20 07 2012 VAHSDOC KONF EE WEEE Conformity This product is subject to the regulations of the EU Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 It is marked by the following symbol Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device For information on recycling and disposal companies in Germany and on the products of Thermo Fisher Scientific which fall under the RoHS Directive Restriction of the use of certain hazardous substan
105. umber of runs Rotor Management Thermo Scientific MX Plus Series 2 61 2 Operation 1 Registering the rotor Step Touch Panel Operation Screen Displays and Notes 1 Procedures for registering the rotor are described as follows RUN Screen MENU 01 Dec 2009 13 43 a The Rotor Rotor Management Eg Management screen Name SIN Hours Runs Last run appea rs a In the ADMIN screen press the Rotor No registered rotor Management icon RUN screen Menu b The ROTOR CATALOG Rotor Catalog screen appears b Press the Add rotor button iin S140AT 140000 10500060 683000 10x 1 00 RA S140NT 140000 752000 607000 8x 2 00 it S120AT2 120000 650000 530000 10x 1 00 em S120AT3 120000 650000 534000 14x 0 50 amn c The Enter S N screen appears c Press the row of the desired rotor name and SR press the Enter button Enter a 4 digit number In the case of 123 prefix zero to the number 0123 d Enter the S N Serial number and press the d Registration is Enter button completed if it is a RUN screen Nent Rotor Management x new rotor having no Name S N Hours Runs Last run S1SOAT 0123 0 o operation history 2 62 MX Plus Series Thermo Scientific Operation 2 Procedures for entering the total operation hours of the rotor are described as follows RUN Screen MENU e The total operation Rotor Management hours of a rotor having operation history can be a In the Rotor Management screen press
106. up to the set speed the device might enter a waiting for vacuum state Therefore before press the START button press the VACUUM button to evacuate the rotor chamber sufficiently aprox 15 minutes Check that the displayed vacuum is at a high vacuum level 3 bars and then press the START button Thermo Scientific MX Plus Series 2 13 2 Operation Figure 2 5 summarizes the operational procedure START Turn on the POWER switch Open the chamber door and install the rotor Close the door and set the For information on how to set a E a a run conditions the run conditions see section SES PE Setting Run Conditions Press the VACUUM button Press the VACUUM button Both of the vacuum pump and temperature control turn off 1 Vacuum pump is ON l Geen Se SSC Air vent opens and the door lock is released 2 If the sample in the rotor is sensitive to a temperature increases wait until the rotor chamber is at a high vacuum level Open the chamber door remove the rotor then turn Press the START ultracentrifuge power off button The rotor is rotating at set speed End This step may be omitted in which case pressing the START button later in the procedure turns on the vacuum pump and the rotor stays at 5000 rpm to wait for chamber vacuum to reach its medium level Figure 2 5 Operational Procedure 2 14 MX Plus Series Thermo Scientific Operation 2 Acceleration Rate and Dec
107. ut changing to the run e If you change to a run parameter such as SPEED or TIME after calling a program parameters the program you have just called is canceled You must call it again to use it e Pressing the START button rotates the rotor under the run parameters of STEP 1 e When STEP 1 ends the system moves to STEP 2 and STEP 3 automatically Note 1 Step display The PROGRAM button indicator displays steps as follows In this case this program contains three steps In the Run screen the run parameters for the first step in this program are displayed When the system finishes separating step 1 and moves to step 2 When the system moves from step 2 to the final step 3 Thermo Scientific MX Plus Series 2 35 2 Operation Thus you can see at a glance how many steps are stored in the specified memory and which step the centrifuge is following 2 You cannot store a run parameter while in running while the rotor is rotating Always perform this function while not in running 3 To perform a combination of a step mode run with an RTC real time control run see section RTC Real Time Control Operation call a program memory unit then set RTC The system then calculates the total of running times of all steps of the programmed run and calculates the startup time for RTC You therefore cannot call a program memory unit after setting RTC 3 Deleting a program This section describes the procedure for de

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