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DR 2800 - ELICROM

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1. ccccccccccccssseceeeeeseeeeseeeeeeeeseeeeeeeseeaeeeeesnaeeeees 40 5 5 4 Setting Basic Test and Sample Data Settings sesseeeeeree 41 5 5 0 Dai Mle BIGINK E 42 5 9 0 Updating a Barcode TeSt a 5iucesietecaetes eet nhecietetes ec N 42 Section 6 Advanced ObperallOlS ccnn a cod edeai cuts 47 Ord WISER IP FOGKAINS TE EET 47 6 1 1 Programming a New User Method ccccccssccccssseeeceececceeececceueeesseueeessageesseaeeessageeessaes 48 6 1 2 Seleciung a User Progra succede usb ectetur e aa a natus ocu d uude v prede eae Eee de feres 56 6 1 3 Adding Editing and Deleting User programs to the Favorites List 56 6 2 Standard Additions Monitoring Checking Results ccccccccsseeceeeeeeeeseeeeeseeeesaeeeesseeeeeseeeeeas 57 6 2 1 Using the Standard Addition Option 2 5 dabis Ec UH E ae ice ain bs a be RA EUER RP a eins 58 6 3 Single Wavelength Mode cccccssececesececscseeecceeeeescesecesceneesceseeescuseecsuseeesaueeessaseeeseeeeesoeseees 60 6 3 1 Setting Up Single Wavelength Mode cccccccccssseecceeeeccesececceueeesseeecceueeesseueeeseaesesseaes 60 6 3 2 Performing a Single Wavelength Measurement seeeessseesseeeeenneeeennnn 61 sm ai u qeugMse c C a 62 6 4 1 Setting the Reading Mode at Different Wavelengths cccceccceeeeseeeeeeeeeeeeeeeeeeeeeeesneees 62 6 4 2 Performing a Measurem
2. C Alldata 4 5 3 2 Time Course The instrument can store 20 Time Course Data sets The data can be stored manually at the user s discretion after viewing the data 5 3 2 1 Data Storage from Time Course Time Course 1 After a reading is taken in Time Course mode press OPTIONS gt STORE The Store Data List will display 0 000 Abs Time amp Interval Main Menu 2 Press STORE to save the current scan to the highlighted numbered line A scan can also be overwritten 9 IUN 05 07 52 10 560 nm 29 JUN 05 07 52 10 Run z 560 nm Cancel 30 Standard Operations 5 3 2 2 Recalling Stored Data from Time Course wee 1 From the Main Menu press RECALL DATA Select TIME COURSE io display the required data If the program is already running press OPTIONS gt MORE gt RECALL DATA 29 JUN 05 07 52 10 Run 2 560 nm Z9 JUM 5 08 15 46 Run 3 CAO nm 2 Press GRAPH or TABLE to view data in a graph or table Note Press VIEW SUMMARY to return to the Recall Data list 0 000 Abs 2 400 min View Summary View Summary 31 Standard Operations 5 3 2 3 Sending Data from Time Course Time Course 29 JUN 05 07 52 10 Run 2 560 nm 29 JUN 05 08 15 46 Run 3 S60 nm 5 min Scale amp Units Instrument Setup Option1 The files will be automatically sent as CSV files Comma Separated Value to a TCData Time Course Data file which can be processed using a spreadsheet program The file
3. Data 0 3 mW wavelength 650 nm This symbol indicates that the instrument contains a Class 1 laser device Data 0 3 mW X 650 nm 2 2 Overview of Product Function The DR 2800 Spectrophotometer is a visible spectrum spectrophotometer with a wavelength range of 340 to 900 nm for laboratory and field analysis The instrument comes with a complete set of application programs and multi language support The DR 2800 Spectrophotometer contains the following application modes Stored Programs pre installed tests Barcode Programs User Programs Favorite Programs Single Wavelength Mode Multi Wavelength Mode and Time Course Mode The DR 2800 Spectrophotometer provides digital readouts in direct concentration units absorbance or percent transmittance When a user generated or programmed method is selected the on screen menus and prompts direct the user through the test This menu system can also generate reports statistical evaluations of generated calibration curves and perform instrument diagnostic checks Section 3 _ Installation 3 1 Unpacking the Instrument 3 2 Operating Environment CAUTION Only qualified personnel should conduct the tasks described in this section of the manual The DR 2800 Spectrophotometer comes packaged with the following items e DR 2800 Spectrophotometer e External power supply with country specific adapter plugs e Dust cover e Two square glass cells 10 mL matched e 3 cell ad
4. Use the alphanumeric keypad to enter the values Note For faster data entry enter all the concentration values then all the absorbance values or vice versa Press OK and enter the corresponding absorbance value Press OK The entered data are displayed in the table Repeat the sequence for each data point you need to enter To change a value in the table activate the appropriate line press the unit key e g mg l or Abs and enter the changed value via the alphanumeric keypad When the data have all been entered press GRAPH to display the curve that results from plotting the entered data Note The correlation coefficient r is shown on the left below the axes The linear equation corresponds to the standard setting Press NEXT CURVE to display the polynomial 2nd order curve Press NEXT CURVE again to display the polynomial 3rd order curve Press FORCE 0 to change the setting from Off to On The curve now passes through the origin of the coordinate system Press TABLE to display the table again When the table has been completed and the curve type has been chosen press DONE when the graph is displayed or EXIT when the table is displayed Go to section 6 1 1 4 on page 54 Advanced Operations Calibration by Reading Standards User css nen 0 1 Select Read Standards and press NEXT Calibration 2 To enter the standard concentrations in the displayed table press the symbol Use the alphanumeric keypad to e
5. Type A Functions as a stopwatch Helps to ensure that the steps of an analysis are correctly timed e g Timer icon reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program A corrective dilution factor can be entered in order to take account of certain properties The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is 1 corresponding to no dilution Note When a dilution is in effect the dilution icon will appear on the display Note If you are using undiluted samples set the dilution factor to 1 again Chemical Form For some stored programs an alternate chemical form and corresponding text can be selected Edit 0 Modifies an existing program Save as User Program Stores the selected parameters as a User Program see section 6 1 on page 47 Recalls saved measurement data wavelength scans or time courses see section 5 3 on Recall Data page 28 Instrument Setup Basic operation settings of the instrument see section 5 2 on page 23 Dilution Factor Off On 41 Standard Operations 5 5 5 Sample Blank 313 Chromium Turbidity and color in the sample matrix can falsify the results of a After blank value corr photo
6. iniu Em d nen Units Lis er Fim es ee ee Fx Ea Absorbance Formula Absorbance Formula KaAat KzAz RaAat KzAz KsAz KaA4 FKzA zT ERA TEAA Kia fKzAz KaAatKeAz Kole KasA 4 KzA z Kz Az Kaha usg er ae ae Ee nre nen Absorbance Formula N Ki atKzAz KrAr t K4 50 hat Azi hz hat ibi 500 700 600 500 Kat Es Kat Kat aa 1 0000 1 0000 1 0000 1 0000 Units of Measure Select the required unit from the list and press NEXT Note A user specific unit that is not included in this list can be added in the edit program under PROGRAM OPTIONS gt EDIT Absorbance Formula The Absorbance Formula menu is used to define the wavelengths and the coefficients used in the formula The absorbance formula defines the calculation for the multi wavelength measurement Press the appropriate key to edit the input Press the FORMULA key In the displayed list select the formula for the program and press OK to confirm List of available Absorbance Formulas A is the absorbance at wavelength 1 A is the absorbance at wavelength 2 and so on K4 is the factor at wavelength 1 K is the factor at wavelength 2 and so on If a subtraction has to be performed the factors can be entered with a minus sign Wavelength i Multi wavelength Press a A key and use the alphanumeric keypad to enter a wavelength Press another A key and enter the next wavelength If necessary repeat until all the wavelengths for the for
7. work step In the third column the times for each timer are Cancel OK entered in mm ss User n Aen Chemical Form 2 Chemical Form 3 Chemical Form 4 Chemical Forms If Chemical Form 1 has been defined up to three additional jp alternative forms can be entered 1 Highlight the appropriate line in the overview of the program data and press EDIT 2 Select or deselect the check boxes to activate or deactivate the chemical forms 3 Press the left key to enter another chemical form with the alphanumeric keypad and press OK to confirm Press the right key to enter the conversion factor to calculate the concentration of the additional chemical form from the concentration of Chemical Form 1 and press OK to confirm 4 Press STORE to save the program data Press CANCEL to return to the Main Menu 55 Advanced Operations 6 1 2 Selecting a User Program User Programs 950 Multi Wavelength 951 Single Wavelength Program Options From the Main Menu press USER PROGRAMS to view an alphabetical list of user programs with program numbers The User Programs list will appear Note Highlight the selection by pressing it or press SELECT BY NUMBER to search for the program by number Press START to run the program 6 1 3 Adding Editing and Deleting User programs to the Favorites List The most frequently used tests methods in the user program menu can also be added to the list of favorites to simplify selection
8. 6 6 4 Output Checks 6 6 5 Lamp History Sy str i t ls iom m lm z Lamp History VISIBLE Hours 0 12 S err la sale y Service Code 6 7 Favorite Programs Advanced Operations From the System Checks menu press OUTPUT CHECKS If a printer is connected a test printing of the current screen will be printed The Lamp History menu provides the amount of time that the lamp has been on Hours From the System Checks menu press LAMP HISTORY After a lamp is replaced the display of the total operating time is reset to 0 1 Press RESET VIS and the Visible Lamp will be reset A system check is performed The duration of the check is 6 minutes 2 Press OK to return to System Checks The Factory Service menu is password protected This menu is not intended for customer use The most frequently used tests methods in the Stored Programs menu and the User Programs menu can also be added to the list of favorites to simplify selection To add Stored Programs and or User Programs to the favorites list or the favorite programs see section 6 1 3 on page 56 71 Advanced Operations 6 7 1 Recalling a Favorite Program Favorite Programs 10 Aluminum Alurnin 0 800 mg L 45 Boron LR Select by Remove Number Program 6 7 2 Deleting a Favorite Program Favorite Programs O AS Boron LR 950 User Program Select by Remove Number Program 72 From the Main Menu press FAVORI
9. UNIT key to select the units for concentration measurements or to create a new unit 3 Press OK to confirm Selecting the Concentration Resolution 1 From the Options menu press CONCENTRATION RESOLUTION 2 Select the resolution and press OK 6 3 2 Performing a Single Wavelength Measurement Single Wavelength Tree Insert the blank cell into the cell holder Press ZERO Note The READ key is only active after the zero measurement has Q 3 4 O been performed E Abs 9 Insert the sample cell into the cell holder Press READ 3 For data storage see section 5 3 1 on page 28 29 JUN 2005 12 56 35 Main Menu Options 61 Advanced Operations 6 4 Multi Wavelength Mode In the multi wavelength mode absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums differences and relationships 6 4 1 Setting the Reading Mode at Different Wavelengths 1 From the Main Menu press MULTI WAVELENGTH 2 Press OPTIONS for Parameter Setup Multi Wavelength Options Concentration Concentration Factor Off Resolution 0 01 Absorbance Save as Formula User Program haoo Asoo Instrument Setup Table 10 Multi Wavelength Setup Options Option Deempion oooo With STORE ON selected all measurement data are stored automatically With STORE OFF Store Off On selected no measurement data are st
10. name will be formatted as TCData X csv X number of scans 1 20 1 From the Main Menu press RECALL DATA and then TIME COURSE to send the data to a USB memory stick or to a printer 2 Press OPTIONS and then the PC PRINTER icon to print the data Option 2 The files will be automatically sent as CSV files Comma Separated Value to a TCData Time Course Data file which can be processed using a spreadsheet program The file name will be formatted as TCYear Month Day Hour Minute Second CSV Time Course Data 1 Press TIME COURSE and then OPTIONS MORE SEND DATA to send the data to a USB memory stick or to a printer 2 Press OPTIONS and then the PC PRINTER icon to print the data 5 3 2 4 Deleting Stored Data from Time Course Time Course 29 JUN O5 07 52 10 Runi 560mm 32 1 From the Main Menu press RECALL DATA and then TIME COURSE or from the Options menu MORE gt RECALL DATA A listing of the stored data is displayed Highlight any data to be deleted 3 Press DELETE in the Options menu and press OK Standard Operations 5 4 Stored Programs The DR 2800 Spectrophotometer contains more than 200 programmed procedures that you can access through the Stored Programs menu 5 4 1 Selecting a Stored Program 1 From the Main Menu press STORED PROGRAMS to view an alphabetical list of stored programs with program numbers Stored Programs 1B luminum Alumun 0 800 rg L 2 Select
11. of the sequence using the alphanumeric keypad Press OK to return to the Instrument Setup menu 7 The Sample ID is activated Each Sample ID is automatically numbered in ascending order after a measurement The number is shown in parentheses behind the Sample ID Note To remove a Sample ID highlight the ID and press DELETE Note Alternatively a Sample ID can be entered or changed in measurement mode In the results screen press OPTIONS gt MORE gt INSTRUMENT SETUP If a Sample ID is already assigned select the Sample ID symbol in the results screen Standard Operations 5 2 3 Setting the Date and Time From Instrument Setup press DATE amp TIME Instr I ee ee 1 Date amp Time 2 he date and time are subdivided over a number of fields Press the appropriate field and use the arrow keys to change the highlighted value 3 Press OK to confirm The instrument will return to Instrument Setup 5 2 4 Setting the Display and Sound Preferences 1 From Instrument Setup press DISPLAY amp SOUND The Instr Display E following options will be displayed f a LAT IL Display Contrast e Display Contrast Adgjusts the display contrast to suit lighting conditions and viewing angle e Screen Touch Activates a short beep each time the Sine screen is pressed Default off Screen touch e Reading Done Activates Deactivates a sound when a Iv Reading done icd reading is complete Default s
12. six precision glass filters target values and instructions is available as an aid for carrying out comprehensive in house instrument checks 6 6 3 1 Wavelength Check Optic mad Oh m m lm E Wavelength Check Wavelength 807 nm Please insert sample cuvette and touch Start i a Ali m amla am Optic Wavelength Check Wavelength 807 nm Peak 806 4 nm 6 6 3 2 Absorbance Check Optic iN TIL m Absorbance Check Wavelength 546 nm Please remove sample cuvette and touch Zero Optic al Akasaka E Absorbance Check Wavelength 546 nm Reading 1 of 5 Advanced Operations The Wavelength Check test is used to check wavelength accuracy at 807 nm The wavelength check requires the Filter Set Cat No LZV537 1 2 From the Optical Checks menu press WAVELENGTH CHECK Insert Adapter A for 10 mm rectangular cells in Cell Compartment 2 and insert the sample cell Neodym or BG20 2 in the adapter Close the cell compartment Touch Start The result is displayed and is to be compared with the nominal data of the sample cell Press CANCEL to return to Optical Checks The Absorbance Check test is used to test the photometric accuracy and repeatability of the instrument 1 2 3 4 From the Optical Checks menu press ABSORBANCE CHECK Press A to input the wavelength Input the wavelength and confirm with OK Remove any cells from the cell
13. than gt Greater than lt Less than or equal to gt Greater than or equal to IF THEN ELSE When the evaluation formula C1 has been entered completely press OK to confirm Press OK again to return to the Formula display When the formula C1 has been entered and confirmed the parameter name upper and lower limits of the measuring range and display result yes no can be entered Entering the Next Calibration Formula C2 or Cn EE Formula 1 Select C2 Off and press EDIT 2 Select C2 Off again and press EDIT The display switches to C2 On 3 Activate the next line C2 to define the formula and press EDIT In addition to the keys described in Table 16 only one other function is available 95 Free Programming esa Select Conc Key ial Select Conc 1 1 If an already defined formula in this case C1 is to be taken into account in the formula for C2 press SELECT CONC Enter the number of the formula e g 1 for C1 and press OK to confirm Cn can now be linked with a mathematical operation Note The Cn concentrations that are to be calculated are numbered in sequence C1 C2 C3 etc Note When the first Cn formula has been defined the Formula list is automatically extended by Cn 1 See Table 17 on page 96 for variable definitions 1 Select the Variables line in the data overview and press EDIT 2 Select the variable to be edited press EDIT and use
14. the following equation Concentration Conc factor Abs reagent blank value Save as User Program Stores the selected parameters as a User Program see section 6 1 on page 47 Recall Data Recalls saved measurement data wavelength scans or time courses see section 5 3 on page 28 Instrument Setup Basic operational settings of the instrument see section 5 2 on page 23 5 4 3 Using Program Timers Some procedures do not require the use of timers Other procedures require several timers These timers are pre programmed into each Stored Program along with a description of the activity to be performed during the timed period 10 oo 1 Press the TIMER icon on the display E Timer 2 Press OK to start the first timer The timer will count down on Invert Timer 01 00 the screen C Shake Timer 00 30 E 3 To start the next timed activity for the Stored Program press the TIMER icon and OK The Timer will beep at the end of the General Timer running time C Timer 15 00 Note To view the Program screen while the timer is running press CLOSE The time will be shown on the bottom left instead of the date Note If necessary press CANCEL to terminate the timer midway through the countdown 34 10 Ale ee ee d ee ee Timer mm s5s 5 4 4 Setting the Dilution Factor S22 nnm Options Standard Operations A general purpose timer is also available in many programs When the timer icon is vi
15. the performance of the instrument 4 Check the reagents Repeat the standard additions using fresh reagents If the results are now good the original reagents were probably bad 5 If nothing else is wrong the standard is almost certainly bad Hepeat the standard additions with a new standard 6 Ifthe problem persists contact Technical Support 57 Advanced Operations 6 2 1 Using the Standard Addition Option 9 Aluminum ECR i 1 From the Main Menu select STORED PROGRAMS Select the required programs Press START 3 Analyze a sample without added standard solution in accordance with the instructions in the Procedures Manual When the measurement is complete leave the sample cell in the cell holder 29 JUN 2005 08 07 28 4 Press OPTIONS gt MORE gt STANDARD ADDITION E Se Dilution ata Factor Off Standard Standard Additions Adjust Off Chemical Form Reagent Blank S Off Instrument An overview of the data of the standard addition procedure is displayed Standard Concentration 5 Press OK to accept the standard values for standard 50 00 mg L AI concentration sample volume total and standard addition empire volume Press EDIT to change any of these values 50 0 mL Spike Volumes mL 0 0 0 1 0 2 0 3 Cancel Edit OK 6 Press the key of the value you want to change Use the Standard Additions alphanumeric keypad to change the value and press OK gt NEXT Optic Standard Co
16. the program number by name or use the arrow keys to scroll through the list quickly and highlight the program or press SELECT BY NUMBER to search for a specific program number Use the alphanumeric keypad to enter the test number and press OK 3 Press START to run the program After a program is selected the screen for that parameter will appear The wavelength does Select natal EERE not need to be selected Number Options 4 Follow the chemical procedures described in the corresponding Procedures Manual 5 4 2 Stored Program Options 1 From the Main Menu select STORED PROGRAMS Choose the desired method and press START 2 Press OPTIONS for Parameter Setup Refer to Table 3 for stored program descriptions 10 Aluminum Alumin Dilution Factor Off Standard Standard Additions Adjust Off Chemical Form Reagent Blank Off 29 JUN 2005 08 18 13 Main D Instrument Menu i Y Table3 Stored Program Options With STORE ON selected all measurement data are stored automatically With STORE OFF selected no measurement data are stored owitches to 96 transmittance concentration or absorbance readings Sends data to a printer computer or USB memory stick Type A Functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g Timer icon reaction times wait times etc can be exactly specified When the specified time has elapsed an audible signal is emitted
17. wavelength mode absorbance Abs or percent transmittance 9e T is measured at up to MULTI WAVELENGTH four wavelengths and absorbance differences and absorbance relationships are calculated Simple conversions into concentrations can also be carried out TIME COURSE The time scan records the absorbance or 96 transmittance at a wavelength over a defined time SYSTEM CHECKS The system checks menu offers a number of options including optical checks output checks lamp history and instrument update RECALL DATA Stored data can be called up filtered transmitted and deleted In this mode user specific or method specific settings can be entered Operator ID Sample ID Date ae eae amp Time Display amp Sound Power Management PC amp Printer and Password 22 5 2 Instrument Setup Mode Instrument Setup Sample ID Printer ae aE Standard Operations 1 Select INSTRUMENT SETUP in the Main Menu A number of options are displayed to configure the basic instrument settings Use this option to enter up to 30 sets of operator initials up to five characters each into the instrument This feature helps record which operator measured each sample 1 From Instrument Setup press OPERATOR ID 2 Press NEW to enter a new Operator ID 3 Use the alphanumeric keypad to enter a new Operator ID Note Spaces cannot be used in this function Use underscore symbols instead on the key YZ_ 4 Pres
18. Cell Compartment 2 The instrument needs to be at the Main Menu or press BARCODE PROGRAMS in the Main Menu Insert the sample cell or blank depending on the working procedure into the cell compartment After Program not available a data overview is displayed including the specifications of the test that is to be revised The working procedures of the corresponding test contains the new data Wavelength Factors Measurement Ranges Conversion Factor for chemical Form etc Highlight the corresponding line including the specifications of the test that is to be programmed and press EDIT When finished programming press STORE and then CANCEL to return to the User Programs menu Press START to start the test with the new data 45 Visit us at www hach com Section6 Advanced Operations 6 1 User Programs User programs provide the opportunity of performing made to measure analyses The User Programs database is empty when the instrument leaves the factory and is used to accommodate programs created by users for specific needs Here are a few examples of entries e Programming of user created procedures The analysis procedure must be developed first before it can be programmed The user must define or determine the program sequences calculation formulas measurement wavelengths factors measuring range limits etc e Modified tests e Assignment of user programs to the favorites menu for frequently used test
19. D Catalog Number DOC022 53 00720 DR 2800 USER MANUAL September 2005 Edition 1 Hach Company 2005 All rights reserved Printed in the U S A te dk Visit us at www hach com Table of Contents S clion T SSDecIIiCallOfS 2 deret m s a ia dede tes ec fune uad 5 Section 2 General Informallon eio e IE e ra dot dada seb etu taeda ce unl ieteudus 7 2 1 DANO LY Inlorralloh sae Sane uicti E ade iui a e r E tU EueTS 7 2 NEST xr ediiedtuiie rec c a ine nane anuiadee T 2 42 Precautionary Lapes aee a optet uice uu iai Lese pli tell E R 7 AWS GIASS A LASER nei Et 8 2 2 IVEIVIEW Or P FOOUCH FUNCION c aseiue2ateiedtaekentintshaeideead node a S esaet 8 Section 9 InstallallOn aeons A rete ce er 9 3 1 Unpacking te st TIGE racea tr E betae oR Hb des uet tects e e Coo Een Eres tome iudice 9 3 2 Operating EhviFODFiell uoto ote ted ta ero a tab Coated tese ues a turco Nba du doit uet 9 9 9 POWELL CODUDOOUONDS scteisotaieh e t pr OUEX aue aad mi pter tic Dreier O edt m scu eua E 10 94 aller OW Cl astutus utu tus iuduieiestaa bua uite etii tota Desrete LEN oia tApe do a ote udi etat 10 3 5 Serial Printer and Personal Computer PC Connections cccccceeecceseeeeeeeeeeeeeseeeeeeaeeeeeeanes 10 3 6 Cell Compartments Cell Adapters Light Shield and Protective Cover 12 3 6 1 Cell Compartments and Adapters cccccccccesecceceeeeeeeeceeeeeeeseaeeeeeseeeeeeeesseeeeesseaeeeseaaae
20. Discrepancy between barcode test data and stored information Data updating Error Program not available Barcode test missing Data updating Please update program data Error The cell cuvette is soiled or there are Clean the cuvette allow the particles to Clean Cuvette undissolved particles in the cuvette settle Error Selfcheck stopped Self Check Test stops while starting the Please check the lamp instrument Please close the lid Check the lamp and replace it if necessary Close the lid and press START AGAIN Error Selfcheck stopped Please remove the cuvette Contact the manufacturer or a sales Hardware error Electronic defect representative Its recommended to execute a Full System Check The instrument needs a new Remove cell close the lid and press Please remove cuvette Full System Check START Please close the lid Negative result The calculated result is negative Check the concentration of the sample Check the programming N a Error in the test database user o evaluation Contact the manufacturer or a sales database representative l The measured absorbance is above the Dilute the sample and repeat the Over measuring range Mp calibration range of the test measurement Please check the lamp The lamp output is too low EU era mprane ie piace Nu necessary ole ee The instrument sensors detect too much Close the lid shade the instrument Move instrument into shade moei or place the protectiv
21. LTIMUM 76 Sections Tro bleshoollIid ee sox end ec tesa esate true nent ed Eua ED abe M o aea Dos 79 Section 9 Replacement Parts and Accessories seseeseeeeseeernee 81 9A Replacement Ail S usse Mes To soU buche anite st du fades dpt LA ET M Cate tate Ch n Dn 81 Seclion 10 HOWTO Order roter ie tertias asso soda EE rom E n DEO 83 Secti n T4 Repair SOIC Casas secede es 84 Section 12 Limited W rranty io ies a ties tanauenieatusideebiestdes 85 Section 1 Specifications Specifications are subject to change without notice Operating Mode Transmittance Absorbance and Concentration 0 005 Abs at 0 0 0 5 Abs 1 at 0 50 2 0 Abs 0 5 at lt 2 Abs 196 at 2 Abs Photometric Accuracy Photometric Linearity with neutral glass at 546 nm 0 296 T at 340 nm with filter KV450 3 lt 0 1 T at 340 nm with NaNO 4 06 kg 8 95 Ib without battery 4 38 kg 9 66 Ib with battery Stray Light Weight Enclosure Ratin IP41 with lid closed IP42 with Protective Cover Operating Requirements 10 to 40 C 50 to 104 F max 80 relative humidity non condensing Storage Requirements 40 to 60 C 40 to 140 F max 80 relative humidity non condensing General Specifications DR 2800 15VDC 30VA output Power Requirements External power supply 100 240 VAC 50 60 Hz input Use only shielded cable with maximum length of 3 m 1 USB for PCs only 1 USB reading device for memory cards a
22. OK Press OK again to confirm the input Note The entered measurement sequence is displayed UseprP 969 Timer Key Timer mm ss P 1 Press the TIMER key to enter any waiting reaction or handling times that have to be taken into account Use the alphanumeric keypad to enter the time and press OK Press OK again to confirm the input Note This time is integrated directly into the measurement process Note The entered measurement sequence is displayed ee R Key Reading Measurement Process 1 Press the R key to program a measurement of the substance that is to be analyzed and press OK 2 Press NEW and then READING and use the alphanumeric keypad to enter the wavelength at which the measurement is to be performed and press OK Press OK again to confirm the input Note The entered measurement sequence is displayed 92 Measurement Process Measurement Process Tlooize Free Programming Key Elements of the measurement sequence that are to be repeated are placed in brackets The left bracket marks the start of the sequence that is to be repeated and the right bracket marks the end Note The key showing the right bracket remains inactive until a left bracket is entered 1 Press 2 Press the key that defines the sequence that is to be repeated Z or R and press OK to confirm 3 Press NEW and then press ZEROING or READING and use the alohanumeric keyp
23. TE PROGRAMS The Favorite Programs list will appear Highlight the selection by pressing it or press SELECT BY NUMBER to search for the program by number Note Use the scroll bar to scroll through the list quickly Note Use the alphanumeric keypad to enter the test number program number and press OK to confirm Press START From the Main Menu press FAVORITE PROGRAMS The Favorite Programs list will appear Highlight the selection by pressing it or press SELECT BY NUMBER to search for the program by number Note Use the scroll bar to scroll through the list quickly Note Use the alphanumeric keypad to enter the test number program number and confirm your input by pressing OK Press REMOVE PROGRAM and press OK Note If a favorites program is removed it will not be deleted from User Programs or Stored Programs Note If a stored program is deleted in User Programs it will also be deleted in Favorite Programs Section 7 Maintenance 7 1 Cleaning Requirements 7 1 1 Spectrophotometer 7 1 2 Display 7 1 3 Cells 7 1 3 1 Glass Cells 7 1 3 2 Plastic Cells DANGER Only qualified personnel should conduct the tasks described in this section of the manual DANGER Always disconnect the power and remove the battery from the DR 2800 before attempting any cleaning operations e Clean the enclosure cell compartments and all accessories with a soft damp cloth A mild soap solution can also be used Do not g
24. The timer has no influence on the measurement program 33 Standard Operations Table 3 Stored Program Options continued Mew cs UTE A corrective dilution factor can be entered in order to take account of certain properties The number entered at the dilution factor prompt will be multiplied by the result to compensate for Dilution Factor Off On the adjustment For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is 1 corresponding to no dilution Note When a dilution is in effect the DILUTION icon will appear on the display E Enables the accuracy of the measurements to be checked The working procedure for a test Standard Additions parameter contains a detailed explanation of how to use this function The working procedure for a test parameter indicates whether a standard adjustment is Standard Adjust necessary and if so how to proceed Adjusts the pre stored calibration for variations between reagent lots or instrument variation i For some stored programs an alternate chemical form and corresponding test range can be Chemical Form o Enables the reagent blank value to be added to or subtracted from the subsequent readings The reagent blank value shifts the calibration curve along the y axis without changing the shape Reagent Blank or gradient of the curve The effect corresponds to a y axis intercept of the calibration straight line This is made clear by
25. USB Interface Cable 1 m USB Keyboard keyboard layout US USB Memory Stick LZV551 LZV647 LZV583 LZV585 LZV584 HYH019 LZV537 LZY274 LZV565 LZV646 9940400 LZV610 LZV642 24954 02 26126 02 21228 00 59405 06 24102 12 LZV566 LZV567 LZV582 LZV568 81 Visit us at www hach com Section 10 How to Order U S A Customers Information Required International Customers By Telephone 6 30 a m to 5 00 p m MST Monday through Friday 800 227 HACH 800 227 4224 By Fax 970 669 2932 By Mail Hach Company P O Box 389 Loveland Colorado 80539 0389 U S A Ordering information by e mail orders hach com e Hach account number if available Billing address e Your name and phone number e Shipping address e Purchase order number e Catalog number e Brief description or model number Quantity Hach maintains a worldwide network of dealers and distributors To locate the representative nearest you send an e mail to intt hach com or contact Hach Company World Headquarters Loveland Colorado U S A Telephone 970 669 3050 Fax 970 669 2932 Technical and Customer Service U S A only Hach Technical and Customer Service Department personnel are eager to answer questions about our products and their use Specialists in analytical methods they are happy to put their talents to work for you Call 1 800 227 4224 or e mail techhelp hach com 83 Section 11 84 Repair Service Autho
26. User Programs 950 Multi Wavelength 951 Single Wavelength Program Options Lis er Nias See a unm Program Options 950 Add to t Der Favorites Edit Delete Cancel Ls er Fiam ee a um Delete Program 950 Program 950 User Program Delete Program 56 1 From the Main Menu press USER PROGRAMS The User Programs list will appear Highlight the selection by pressing it or press SELECT BY NUMBER to search for the program by number Note Use the scroll bar to scroll through the list quickly Use the alphanumeric keypad to enter the test number program number and press OK to confirm Press PROGRAM OPTIONS Press ADD TO FAVORITES EDIT or DELETE and press OK Note If the stored program is deleted in User Programs it will also be deleted in Favorites Programs Advanced Operations 6 2 Standard Additions Monitoring Checking Results Refer to section 6 2 1 on page 58 for instructions on how to perform a standard addition using the DR 2800 The accuracy of measured values their correspondence with the actual concentration of the parameter in the sample and the precision correspondence of the measurement results obtained from several samples containing the same concentration of the test parameter can be determined or improved using the standard addition method This method also referred to as spiking serves to identify sample specific interference factors e g substances in the sampl
27. ad to enter the wavelength at which the measurement should be performed Press OK to confirm 4 Press to end the sequence Note If an action such as a zero measurement recurs at different stages of a measurement sequence the series of actions is numbered sequentially e g Z1 Z2 etc Deleting an element of a measuring sequence Select the appropriate line and press DELETE The element is deleted Inserting an Element of a Measuring Sequence Select the line in the measuring sequence where the insertion is be made and press NEW A new element can be entered at the selected position When the input is complete press OK in the Measurement Process display The data overview is then displayed B 2 Entering the Calibration Formula Evaluation Formula User Program 953 Cancel The calibration formula evaluation formula defines the calculation and display of intermediate and final results The previously defined elements of the measuring sequence are the basis for calculating the concentrations Entering Calibration Formula C1 1 Highlight the Formula line in the data overview and press EDIT 93 Free Programming User Penne ne 2 Select the line C1 Off and press EDIT Formula Select C1 Off again and press EDIT The display changes to C1 On 4 Select the next line C1 to define the formula and press EDIT Refer to Table 16 for detailed information on the Edit formula keys Note The evaluation formu
28. am by number Chlorine Ozone 3 Press ADD TO FAVORITES and press OK to confirm Add to Favorites L 4 The program can now be selected from FAVORITE PROGRAMS on the Main Menu 38 Standard Operations 5 5 Barcode Programs A special barcode reader in the cell compartment automatically reads the coded information on each TNTplus 13 mm vial while the vial completes a single rotation The instrument uses the barcode identification to automatically set the correct wavelength for the analysis and calculates the result immediately with stored calibration information Multiple points around the vial are measured during the rotation Outlier values are recognized and eliminated and the average of the remaining readings is calculated This method increases the precision of the measurement results and minimizes errors due to soiling of the vial Figure 10 Cell Compartment 1 for 13 mm and 16 mm Round Vials 5 5 1 Performing a TNTplus Test 1 Insert the Light Shield in Cell Compartment 2 ZEUG 2 Prepare the TNTplus vial in accordance with the working eor ME procedure o M a When a coded cell is placed in Cell Compartment 1 Proarams Proarams l i TET s the corresponding measurement program is Reading barcode 5 i i activated in the Main Menu Please wait OR b From the Main Menu press Barcode Programs Time Course System Instrument Checks Setup 39 Standard Operations Ba
29. and use the alphanumeric keypad to enter a previously recorded reagent blank value 8 Press OK Note The Reagent Blank function is deactivated when you leave the measurement program To use the same blank value later for other tests using the same reagent lot enter the value per step 7 Note The results calculated using the reagent blank value must lie within the limits of the measuring range of the test method Note The reagent blank icon is shown in the result display see arrow when the function is active 37 Standard Operations 5 4 8 Analysis of Samples 1 Press STORED PROGRAMS and select a program 10 Aluminum Alumin 2 Insert the blank cell into the cell holder 0 0 0 0 3 Press ZERO 29 JUN 2005 08 20 55 e Main Menu Options 4 Remove zero solution cell and insert sample cell into the cell compartment Press READ The result will be displayed Q 1 3 1 5 For data storage see section 5 3 1 on page 28 10 Aluminum Alumin j 522 nm e9 JUN 2005 08 21 08 Main Menu 5 4 9 Adding Stored Programs to the Favorite Programs List The Favorites menu simplifies test selection by creating a list of the most frequently used tests from the Stored Programs and User Programs Stored n 1 From the Main Menu press STORED PROGRAMS The Stored Add to Favorites Programs list will appear TN 2 Highlight the selection or press SELECT BY NUMBER to search Program 210 for the progr
30. apters A B and C e Light Shield e Protective Cover e DR 2800 User Manual e Procedure manual on CD ROM If any of these items are missing or damaged contact the manufacturer or a sales representative immediately Note Retain the original packaging materials Instruments returned for service should be shipped in the original packaging material to protect against damage during transportation The following conditions are necessary to ensure correct instrument operation and accurate results Place the instrument firmly on an even surface Do not push any objects under the instrument Maintain an ambient temperature of 10 to 40 C 50 to 104 F for proper instrument operation The relative humidity should be less than 80 moisture should not condense on the instrument Leave at least a 15 cm 6 in clearance at the top and on all sides for air circulation to avoid overheating of electrical parts Do not operate or store the instrument in extremely dusty damp or wet locations Keep the surface of the instrument the cell compartment and all accessories clean and dry at all times Splashes or spills on and in the instrument should be cleaned up immediately section 7 1 on page 73 Important Note Protect the instrument from temperature extremes including heaters direct sunlight and other heat sources Installation 3 3 Power Connections 1 Adapter Plug US version shown Install the correct adapter p
31. ationships This is an advanced form of programming for original user developed methods In Free Programming the user defines the measurement process variables and calculations involved in obtaining a reading See Appendix B on page 91 for information on Free Programming parameters Free Programming 48 Advanced Operations 6 1 1 1 Single Wavelength Programming Lis er apr T nen Units Us er Vh ini ics iini a nen s Wavelength X nm Ls er ae ee ee nfm Concentration Resolution Lis er ee ee ne Chemical Form ona Selecting Units of Measure Select the required unit from the list and press NEXT Note Units of measure not included in this list can be added in the edit program under PROGRAM OPTIONS gt EDIT Select UNITS gt EDIT gt NEW There is no 7 character available Defining the Wavelength Use the numeric keypad to enter the measurement wavelength The entered wavelength must be in the range from 340 900 nm Press NEXT to proceed Concentration Resolution Select the required number of decimal places from the displayed list and press NEXT Setting the Chemical Form Enter the chemical formula used in the display to represent the analysis parameter Use the alphanumeric keypad to enter the chemical form and press NEXT to enter Calibration settings Go to section 6 1 1 3 on page 51 to continue with Calibration 49 Advanced Operations 6 1 1 2 Multi wavelength Programming Ls er Vh anii
32. ber is already assigned to another user program a message appears asking whether the existing program should be replaced Press OK to overwrite the existing program User eee nen Program Name Program Name 4 Use the alphanumeric keypad to enter a program name The name can be a maximum of 28 characters long Repeat a key prr S press to select another of the three letter options Use the abc J ABC E DEF GHI CE underscore key for spaces Tem eoo JKL MNO PaR q 5 Press BACK to go to the previous program point or press NEXT el B to continue with the input of the program data ner ud mm MaumuumEcwEN Program Type Program Type 6 Select the required option Table 7 and press NEXT Single Wavelength _ 7 Ifthe Single Wavelength section 6 1 1 1 on page 49 or Multi wavelength section 6 1 1 2 on page 50 is selected mm define the unit wavelength absorbance formula C Free Programming wavelength iy concentration factor Ky concentration F resolution chemical form and calibration equation See Appendix B on page 91 for information on Free Programming parameters C Multi Wavelength Table 7 Program Descriptions Single Wavelength Measurements at a defined wavelength In the Multi Wavelength mode absorbance values can be measured at up to four wavelengths Multi Wavelength l l and the results can be mathematically processed to obtain sums differences and rel
33. compartment and press ZERO Insert the sample cell into the cell compartment and press READ Five replicates of blanking and reading lead to results 69 Advanced Operations IEEE 7 The result is displayed and is to be compared with the nominal m Absorbance Check data of the sample cell 8 Press CANCEL to return to Optical Checks Wavelength 546 nm Average 0 2602 Abs Std Deviation 0 0014 Abs 6 6 3 3 Stray Light Check Optic haa The Stray Light test is used to measure the stray light in the m Stray Light Check instrument at 340 nm Wavelength 240 0 nm 1 From the Optical Checks menu press STRAY LIGHT CHECK Remove any cells from the cell compartment Please remove sample cuvette and touch Zero 3 Insert the Adapter A for 10 mm rectangular cells in Cell Compartment 2 Press ZERO Cancel Zero Optic 4 Insert the sample cell into the Cell Compartment 2 Close the n Stray Light Check cell compartment Press START Wavelength 340 0 nm Please insert sample cuvette and touch Start 5 The Average and Standard Deviation are calculated from three Stray Light Check successive absorbance measurements The result is displayed and is to be compared with the nominal data of the sample cell 6 Press CANCEL to return to Optical Checks Optic sl oS mee Wavelength 340 0 nm Average 3 045 Abs Std Deviation 0 0276 Abs Cancel 70
34. consumable components of a product such as but not limited to lamps and tubing Contact Hach Company or your distributor to initiate warranty support Products may not be returned without authorization from Hach Company Limitations This warranty does not cover e Damage caused by acts of God natural disaster labor unrest acts of war declared or undeclared terrorism civil strife or acts of any governmental jurisdiction e Damage caused by misuse neglect accident or improper application or installation e Damage caused by any repair or attempted repair not authorized by Hach Company e Any product not used in accordance with the instructions furnished by Hach Company e Freight charges to return merchandise to Hach Company e Freight charges on expedited or express shipment of warranted parts or product e Travel fees associated with on site warranty repair This warranty contains the sole express warranty made by Hach Company in connection with its products All implied warranties including without limitation the warranties of merchantability and fitness for a particular purpose are expressly disclaimed Some states within the United States do not allow the disclaimer of implied warranties and if this is true in your state the above limitation may not apply to you This warranty gives you specific rights and you may also have other rights that vary from state to state This warranty constitutes the final complete and exclusive
35. e cover over or close the lid id sample cell compartments Self Check Test stops while starting the Remove the cell and press OK instrument If possible select a test with a lower measurement range or use a cuvette with a longer path length The measured absorbance is below the Under measuring range calibration range of the test Close the lid or place the protective cover over sample cell compartments and repeat the measurement Fluctuating ambient light conditions Te itions Unstable lighting conditions during the measurement 79 Visit us at www hach com Section9 Replacement Parts and Accessories 9 1 Replacement Parts Description Catalog Number Battery rechargeable lithium Cell Adapter Set sample cell adapters A B and C Cell Adapter A 10 mm square Cell Adapter B Pour Thru Cell Cell Adapter C 1 inch round Dust Cover Filter set certified for self checks 6 precision glass filters with target values Hach Data Trans PC software for data transfer Lamp tungsten Light Shield Pour Thru Cell Kit Power Supply external Protective Cover adapter holder Sample Cell 10 mL 1 inch glass square matched pair Sample Cell 25 mL 1 inch glass square matched pair with stoppers Sample Cell 10 mL round glass with cap each Sample Cell multi path 10 and 25 mL plastic with caps pk 6 Sample cell 25 mL 1 inch square plastic with cap pk 12 USB Barcode Scanner hand held scanner
36. e that alter the analysis results sample matrix effect a defective measuring instrument or contaminated reagents Standard Additions is performed by adding a known amount of a standard solution to a sample If results are not close to 100 recovery an identifiable problem exists If the use of standard additions is appropriate for a test a Standard Additions method will be in the procedure under Accuracy Check Follow the detailed instructions provided If the results are about 100 recovery for each addition they are likely correct Results can be verified by running a standard solution through the test Poor recovery indicates that a problem exists For example to test for interference in the sample repeat the standard additions using deionized water as the sample to check the reagents instrument and technique If recovery is now about 100 for each addition interference is likely present in the sample 1 For poor recoveries with the deionized water use the following list to find the problem e Follow the procedure exactly e Are the reagents added in the correct order e s enough time allowed for color development e Is the correct glassware in use e Is the glassware clean e Does the test need a specific sample temperature e Is the sample pH in the correct range 2 Consult the procedure in the DR 2800 Procedure Manual to answer these questions 3 Follow the instructions in Troubleshooting on page 79 to check
37. e working procedure in advance to determine whether this point needs to be changed 1 Activate the Variables line in the data overview and press EDIT 2 Select the variable to be edited press EDIT and use the alphanumeric keypad to enter the data specified in the working procedure for F1 F2 A1 U1 etc Confirm each entry with OK Abbreviation of Variables e Ff Factor 1 e F2 Factor 2 e Ad Wavelength 1 e Ut Conversion Factor 1 for the first chemical form e U2 Conversion Factor 2 for additional chemical forms etc Timer 1 Timer 2 Timer 3 Timer 4 The timers are activated or deactivated with the control boxes on the left of the display In the next column a selection can be made from a list of names that describe the corresponding work step In the third column the times for each active timer are entered 5 5 6 2 Updating an Existing Barcode Test Program 342 Marne Mitrit 44 Note Only in very few cases does the revision of a test require all test specifications to be updated Option 1 Manual Update of Test Data 1 Insert the Light Shield in Cell Compartment 2 2 he instrument needs to be at the Main Menu or press BARCODE PROGRAMS in the Main Menu 3 Insert the sample cell or blank depending on the working procedure into the cell compartment 4 After Barcode controlnumber a data overview is displayed including the specifications of the test that is to be revised Note The w
38. en a new formula is selected the number of variables changes to match The following formulas are available K Ay KoAo K A KoAn K3A3 K A KoAn K3A3 Ky4Ay4 K1A4 K2A5 K1A4 K2A5 K5A3 K1A41 K2A2 K3A3 4 K 4A 4 A 4 refers to the absorbance at wavelength 1 A refers to the absorbance at wavelength 2 etc K 4 refers to the coefficient at wavelength 1 K gt refers to the coefficient at wavelength 2 etc Coefficients can be set negative where subtraction is required 3 To change a wavelength press one of the Ax keys Enter the desired wavelength into the numeric keypad Press OK to confirm 4 To change a coefficient press one of the Ky keys Enter the desired coefficient into the numeric keypad Press OK to confirm Note The instrument allows up to 5 significant digits with a maximum of 4 significant digits after the decimal point Setting the Concentration Factor 1 From the Options menu press CONCENTRATION FACTOR OFF Select On to activate this feature 2 Press the Factor key to enter the factor by which absorbance readings are to be multiplied Press the Unit key to select the units for concentration measurements or to create a new unit 3 Press OK to confirm 63 Advanced Operations Selecting the Concentration Resolution 1 From the Options menu press CONCENTRATION RESOLUTION Optic Concentration Resolution 2 Select the resolution and press OK 6 4 2 Performing a Mea
39. ent in the Multi Wavelength Mode sss 64 6 5 Time Course ModE 2 casi tub eode recede bae eC be ata Ub eda a e eu de oram EU CUN 64 6 5 1 Time Course Setup Parameters ccccccccccccseseceeceeseceeseeeeeeeeseeeeceseeeaeeeesseeeeessaeeeeeeseggees 64 65 2 dime Gourse Scan Reading ssssescasessques uiae up cbe et teen e eee 66 6 5 3 Analysis of Time Course Data sssaaa ie iaae aa a a nne nenne nnne nnn nnns nnns 66 Do System one toccato tunc datei NES eee Naan Le MUS M Pea Lee CU dU EE 67 6 61 mMsrument ieirentur iio Pr ees 68 6 6 2 Upgrading the Instrument Software sassi nena r nennen ns 68 66 ODBIG3I GBecKS sessiya aE heo Uta E ven dicens Basta sends EuM d RUE Ue 68 5 54 OBUDUt CMCC i Sires aie E heated at td Si aah ac E LI ae eee ea Ah La e aua 71 6 625 AMO ISIONY MPO TRE OT 71 6 7 AVON Programs IO Eom 71 6 741 Re lling a Favorite Program sieniin nn a e a a 72 67 2 Deleung a Favorite POgEI aliia viceeti ctas udis ican A dote Lese Kee chee co od tn RE Eden pe us DE 72 SECTION 7 Maintenant e oido diet a um Datei bubo a Aa 73 7 Ee iase azote Tm M 73 FAs SSDECIOOMOTOMEICN cease t o S mom 73 Fo BS 6 cl ar 73 r IRS oat E Ae 73 Ze Battery Hepiacemellb circe ta neanqneteaissiuoraiacratenapsulenetlsned eeruentanaducs 74 Fel Charging TNE DAMON Y osea rte b o e ass modas nt ode tute C udi aan De ceased siete 75 Tee alia Mq 75 To LAMP cile zie EID U
40. eraser or a stylus Do not press the screen with a sharp object such as the tip of a ball point pen e Do not place anything on top of the screen to prevent damage or scratching on the screen e Press keys words or icons to select them e Use scroll bars to move up and down long lists very quickly Press and hold the scroll bar then move up or down to move through the list e Highlight an item from a list by pressing it once When the item has been successfully selected it will be displayed as reversed text light text on a dark background 5 1 2 Using the alphanumeric keypad This display is used to enter letters numbers and symbols as needed when programming the instrument Unavailable options are disabled grayed out The icons on the right and left of the screen are described in Table 1 The central keypad changes to reflect the chosen entry mode Continue to press a key until the desired character appears on the screen A space can be entered by using the underscore on the YZ key Instr een ant Cede Operator ID ee tes at Sample ID BH te ac or on E EXESESESES s e on v Cancel OK Cancel OK Table 1 Alphanumeric Keypad Functions eon Description ABC Alphabetic When entering alphabetic characters e g user entered units toggles between upper and lower case letters Symbols Punctuation symbols and numerical sub and superscripts may be entered Enters regular numbers Clears t
41. et excess water in the cell compartments Do not insert a brush or sharp object into Cell Compartment 1 to avoid damaging the mechanical components e Dry the cleaned parts carefully with a soft cotton cloth Important Note Under no circumstances should the instrument display or the accessories be cleaned with solvents such as petroleum distillates acetone etc e Take care not to scratch the display Do not touch the screen with the tip of a ball point pen pencil or similar pointed objects e Clean the display with a soft lint free and oil free cotton cloth Diluted window cleaner can also be used CAUTION Use proper laboratory practices whenever there is a risk of chemical exposure Important Note Glass cells that have been used for organic solvents such as chloroform benzene toluene etc must be rinsed with acetone before being treated with cleaning agents In addition another rinse with acetone or water is necessary as a final treatment step before the cells are dried e Clean glass cells with cleaning agents and water e Avoid scratching the optical surfaces with brushes or other cleaning devices e Afterwards rinse the cells several times with tap water and then thoroughly with deionized water e Clean with detergent and water or with mild acid brief exposure e Avoid scratching the optical surfaces with brushes or other cleaning devices e Rinse with deionized water 73 Maintenance 7 2 Bat
42. etting from Off to On The curve then passes through the origin of the coordinate system 11 Press TABLE to display the table again 12 When the table has been completed and the curve type has been chosen press DONE when the graph is displayed or EXIT when the table is displayed Go to section 6 1 1 4 on page 54 53 Advanced Operations Ls er Fiam insi E a nen Calibration Calibration by Entering the Formula 1 Select Enter Formula and press NEXT Enter alues Read Standards OO SS LL LLLLLLLLL LLL ILIILIIIIIILLLILLLLLZLZLLLLLLLLLXLLLL amanis Enter Formula TEE 2 Press the formula key key that contains an equation Enter Formula A list of available formulas linear and 2nd and 3rd order Its polynomial is displayed Up to 4 coefficients can be entered dais z depending on the selected formula Press the required formula b 3 Depending on the selected formula the required coefficients a A 1 0000 m l ui b c are displayed Press the coefficient keys and enter the B corresponding values via the alphanumeric keypad After each entry press OK to confirm Note The coefficients can have up to 5 digits and can have a positive or a negative sign micas Loc The input of the basic method data is now complete An overview of Name Nitrate the variable program data is displayed 1 Toenter more specifications or chan
43. ettings is used to search for specific items Activate On to turn on the filters to select data by Sample ID Operator ID Start Date Parameter or any combination of the four Press OK to confirm The chosen items are listed T Press VIEW DETAILS to get more information 5 3 1 3 Sending Data from the Data Log Send Data Single point C Filtered data 2 C Alldata 4 Data is sent from the data log as CSV Comma Separated Value files through a USB memory stick to a file named DATALOG The file can then be processed using a spreadsheet program The file name will be formatted as DLYear Month Day Hour Minute Second CSV To send data to a printer refer to section 5 2 6 2 on page 27 1 Plug in the USB device Figure 2 on page 11 2 From the Main Menu press RECALL DATA Press OPTIONS and then the PC amp PRINTER icon 3 Select the data to send to the memory stick and press OK Note The number in parenthesis is the total number of data sets assigned to this selection 29 Standard Operations 5 3 1 4 Deleting Stored Data from the Data Log 3 5 1 From the Main Menu press RECALL DATA 2 Press DATA LOG gt OPTIONS and then DELETE 3 Activate Single Point or Filtered data or All data and press OK Delete Data Single point Note The number in parenthesis is the total number of data sets assigned C Filtered data 2 to this selection
44. ews Figure 13 on page 77 11 Remove the halogen lamp with the plug panel 12 Carefully unplug the halogen lamp from the plug panel Important Note Hold the lamp by the base only To prolong lamp life do not touch the glass 13 Plug a new halogen lamp Cat No LZV565 into the plug panel 14 Insert the halogen lamp into the lamp housing with the rounded part of the lamp base pointing down 15 Secure the lamp firmly with the two knurled screws 16 Carefully insert the lamp housing into the instrument 17 Use a screwdriver to replace the screws securing the lamp fitting 18 Reinstall the battery and cover see section 7 2 on page 74 19 Use a screwdriver to replace the screw securing the cover 20 Carefully stand the instrument upright 21 Plug in the power supply The Instrument is now ready for use Maintenance 22 Reset the Lamp History see section 6 6 5 on page 71 Figure 12 Removing the Lamp 2 Lamp Housing Screw 2X Figure 13 Replacing the Lamp 1 Halogen Lamp 3 Plug Panel 2 Lamp Base 4 Rounded Part of Lamp Base T7 Visit us at www hach com Section8 Troubleshooting Problem Display screen Likely Cause Action 0 Absorbance gt 3 5 The measured absorbance exceeds 3 5 UE Dip SAMPE ARE TEPER INE measurement Calculated concentration is higher than Dilute the sample and repeat the Concentration too high 999999 measurement Error Barcode control number Please update program data
45. f the measurement result is carried out automatically Note The selected Chemical Form will appear on the display Test results will be calculated and displayed in this chemical form Alternatively the standard setting can be changed by pressing OPTIONS gt MORE gt CHEMICAL FORM Select the required form and press OK 5 4 7 Running a Reagent Blank 10 Alm a err e Reagent Blank 22 nm m Adjust to a 0 000 mg L AI 10 Aluminum Alumin 522 nm 0 131 7 zU JMIM z 005 C S zD 4d ilh Opti ons Standard Operations some of the stored tests methods include the Reagent Blank function This enables the reagent blank value to be measured and then taken into account in calculating the measurement result Measurement Analysis of a Reagent Blank 1 Prepare the Test Method as specified with the working procedure Instead of a sample distilled water is used to determine the reagent blank value 2 Select the test If required by the working procedure place the zero solution in the cell compartment Press ZERO 3 Place the prepared cell in the cell compartment and press READ The result is displayed 4 Press OPTIONS MORE REAGENT BLANK Press On to activate the Reagent Blank function The concentration shown on the key is the measured value of the reagent blank To use this value for more analyses of this parameter press OK T Ifthe measurement does need to be saved press the key
46. ge existing information highlight the appropriate line and press EDIT 2 Select STORE to save the user program Lower Lirnit Off Timer 1 Off E 6 1 1 5 Additional User defined Parameters and Functions In addition to the previously defined basic data additional parameters and functions can be defined for user programs e measuring range upper and lower limits e timer functions e chemical forms 54 Advanced Operations TELE mm Upper and Lower limits of the Measuring Range Enter a maximum upper and minimum lower measurement value to define when an error message is displayed for readings above the upper limit or below the lower limit 1 Highlight the appropriate line in the overview of the program data and press EDIT 2 Select On and press the 0 000 key to enter the measuring range limit using the numeric keypad Press OK to confirm UserP nra Timer 1 Timer 2 Timer 3 Timer 4 nm This function can define time intervals for up to four timers Timer ts NND mm designations such as Shake Wait and Swirl can be assigned E X ms 1 Highlight the appropriate line in the overview of the program qi 2 PIDE pode data and press EDIT oS Timer 00 00 2 The timers are activated or deactivated with the check boxes in zi the left part of the display In the next column a selection can memi a Timer 00 00 be made from a list of names that designate the corresponding
47. h measurements are carried out the number of absorbance measurements needed the keys to be used any waiting periods between measurements etc Measurement process Definition of the formulas with which the test result is calculated The number of variables shown in the display depends on the definition of the measurement Variables process and the formulas Input of the numerical values of the wavelengths factors constants etc This function can be used to enter abbreviations and defined times for up to four timers Activate the appropriate line and press EDIT The timers are activated or deactivated with the control boxes on the left of the display In the next column a selection can be made from a list of names that describe the corresponding work step In the third column the times for each active timer are entered Timer 1 Timer 2 Timer 3 Timer 4 42 Program 342 Marne Mitrit de oe oer om ien EXESESESES ime 323 stu vwx vz Prog oh Lu E om Measurement Process Standard Operations Enter the Name Check the working procedure in advance to determine whether this point needs to be changed 1 Highlight the line containing Name and press EDIT Use the alphanumeric keypad to enter the name indicated in the working procedure 2 Press OK to confirm Enter the Versi
48. h the plastic windows Appendix B Free Programming Free Programming is an advanced option for entering original User Program 950 user developed methods When the Free Programming option is Mame CK selected an overview of the specifications of the programmed test Versions o Se et eee is displayed Each input option can be modified to develop the user Measurement Process 4 method Refer to Table 15 for more information To modify an input option select the appropriate line and press EDIT Important Note Perform steps 1 6 of Programming a New User Method section 6 1 1 on page 48 before proceeding with Free Programming Cancel Table 15 Free Programming Options Name of the analysis parameter An abbreviation or version number assigned by the user is entered here Exact definition of the test the number of wavelengths at which measurements are made the Measurement Process number of absorbance measurements needed the keys to be used any waiting periods between measurements etc Definition of the formulas with which the test result is calculated The number of variables shown in the display depends on the definition of the measurement process Variables and the formulas Input of the numerical values of the wavelengths factors constants etc Used to enter abbreviations and defined times for up to four timers Activate the appropriate line and Timer 1 Timer 2 touch EDIT The timers are activated or deactivated wi
49. he entry Moves back one position Deletes the character previously entered in the new position Moves to the next space in an entry when two adjacent characters occur on the same key 21 Standard Operations 5 1 3 Main Menu A variety of modes may be selected from the Main Menu Table 2 n n briefly describes each menu option Barcode Programs Favorite Programs System Instrument Checks Setup Table 2 Main Menu Options Stored programs are pre programmed methods that make use of reagents cell tests and STORED PROGRAMS pipette tests BARCODE PROGRAMS Refer to the DR 2800 Procedure Manual for step by step procedures for analyses using stored programs User programs make made to measure analysis possible Users can program methods they have developed themselves Stored methods can be saved as user programs The tests can then be modified to suit the user s requirements FAVORITE PROGRAMS List of frequently used methods tests selected by the user Single wavelength measurements are Absorbance measurements The light absorbed by the sample is measured in absorbance units USER PROGRAMS Transmittance measurements 96 The percentage of the light that passes through the sample and reaches the detector is measured Concentration measurements A concentration factor can be entered to enable the measured absorbance values to be converted into concentration values SINGLE WAVELENGTH In the multi
50. hort beep every time a Timer Short Long ane reading is complete Cancel OK e Timer Adjusts the length of the timer sound Select Short or Long Long beeps are recommended for noisy environments 2 Press OK to confirm The instrument will return to Instrument Setup 5 2 5 Setting the Power Management Options The power management option places the instrument in a standby mode or shuts the instrument off automatically after an idle period to prolong battery life The battery is an optional accessory and is not included with the instrument epe 1 From Instrument Setup press POWER MANAGEMENT The Power Management battery symbol indicates the battery life in 96 SSSI ok Note The timer settings are only available when the instrument is using 5 Minutes battery power Auto off 25 Standard Operations z 2 Select one of the options under Standby mode to set the length Standby for Battery Operation of the idle period that can elapse before the instrument switches to the Standby mode Press OK In J lE tT D M P JE issuu Note In Standby mode the instrument backlight is turned off To return the 30 Seconds instrument to active operation touch anywhere on the display 1 Minute 5 Minutes 3 Select one of the options under Auto off to set the length of the Auto off for Battery Operation idle period that can elapse before the instrument automatical
51. ion DR 2800 S M 1137297 Instrument Version 0 25b Hach Programs Version 02 Lange Programs Version 53B 1 Press INSTRUMENT INFORMATION 2 he model serial number and software version are displayed 6 6 2 Upgrading the Instrument Software S zr Fu oe els Hida Instrument Update Please insert an Instrument Update Module inta a USB Port or connect to 4 computer that is running the update program const o 6 6 3 Optical Checks Optical Checks Wavelength Absorbance Check Check Stray Light Check 68 Obtain the software for the update from the Internet at www hach com 1 Go to http www hach com 2 Onthe DR 2800 product page click DOWNLOADS gt SOFTWARE Locate the appropriate download and follow the prompts for saving the file s to the USB memory stick On the DR 2800 press INSTRUMENT UPDATE 5 Connect the USB stick to the USB interface on the DR 2800 Figure 2 on page 11 Press OK The link is established automatically and the software is updated 6 Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed 1 From the System Checks menu press OPTICAL CHECKS The Optical Checks menu contains programs for checking the wavelength accuracy stray light and photometric accuracy An optional test aid kit Cat No LZV537 containing
52. ittance Sends data to a printer computer or USB memory stick Recalls saved measurement data wavelength scans or time courses section 5 3 on page 28 Instrument Setup Basic operational settings of the instrument section 5 2 on page 23 Setting the Time and Interval ne TEGEE Time amp Interval 1 From the Options press TIME amp INTERVAL Total Time Fa 2 Input the total time and the reading time and press OK 5 Minutes Note In total 500 measuring steps are possible If a total time and a time interval is selected that would cause this number of measurements to be exceeded the time interval is defined automatically and the OK key is Reading Interval inactivated 12 Seconds Setting the Scale and Units Scale Units l 1 From the Options menu press SCALE amp UNITS 2 Select Abs or T as the required units i Abs zale 3 Select Auto or Manual scaling on the graph s y axis Auto C Manual Note If manual scaling is selected use the alohanumeric keypad to set the limits Ymin and Ymax The graph is adjusted to display only the values in the 0 300 Abs 3 500 Abs selected range If automatic scaling is selected the instrument sets the limits automatically so that the total range can be displayed 4 Press OK to confirm b Press RETURN to return to the scan mode 65 Advanced Operations 6 5 2 Time Course Scan Reading Time Course j 560 nm Options Time Co
53. la is built successively in the display in accordance with the input Note The ARROW key deletes the most recently entered element of the formula Table 16 Edit Formula Key Descriptions Key Description Press the SELECT ABS key to select from the displayed list the required element of the defined measuring ABS VARIABLES sequence and therefore the corresponding measurement wavelength so that this can be taken into account in the formula New Number NEW NUMBER Press NEW NUMBER to enter a new factor or constant 94 Free Programming Table 16 Edit Formula Key Descriptions continued Press to enter a mathematical operation Select the ADIT D i en User Math operator operation and confirm your input by pressing OK The available choice of mathematical operations depends on the defined formula This means that functions such as or In log etc are only active if a term in parentheses or the calculation of a logarithm is mathematically permissible in the defined formula this also applies to all further basic mathematical operations The following basic mathematical operations are available Addition Subtraction Division Multiplication Exponent Ln Natural logarithm Log Common logarithm ee Press gt lt to include logic statements links conditions in Conditional operator the formula The following functions are available Equal to lt Less
54. lation 2 Press the Protective Cover lightly into the correct position until the cell compartment is completely sealed and the cover cannot slip off Figure 9 Protective Cover Installed 17 Visit us at www hach com Section 4 Start Up 4 1 Powering the Instrument On and Off 4 2 Language Selection Main LI P Language English 4 3 System Diagnostics Main MA mmt DR 2800 Self Check STSTEM TESTS LAMP TESTS FILTER ADJUSTMENT CALIBRATION mb VOLTAGE TESTS 1 Plug the external power supply into an electrical outlet or insert the battery 2 Press the On Off switch for approximately one second to power on the instrument 3 Press the On Off switch for 3 to 5 seconds to turn the instrument off An acoustical signal confirms that the instrument has been powered off Note Do not switch the instrument off and on in rapid succession Always wait about 20 seconds before switching the instrument on again otherwise you may damage the electronic and mechanical systems The DR 2800 Spectrophotometer software includes several language options The first time the instrument is powered on the language selection screen will appear 1 Select the desired language 2 Press OK to confirm the language selection The self check will start automatically Note Once the language is selected the instrument will power up in that language until a different language is selected To change the language after initial ins
55. ld be legible on the adapter Figure 5 Note The arrow on top of the adapter indicates the direction of the light beam path 13 Installation Figure 5 Installing a Cell Adapter 14 Installation 3 6 3 Using the Light Shield for TNTplus or Test N Tube Measurements The Light Shield Figure 6 prevents light interference when using Test N Tube or TNTplus vials and must be in place before measurements can be taken in Cell Compartment 1 The Light Shield is required only when using Test N Tube or TNTplus vials The DR 2800 is shipped with the Light Shield installed Remove the Light Shield before using Cell Compartment 2 The Light Shield can be stored in the Protective Cover Figure 8 on page 16 Figure 6 Light Shield Cat No LZV646 Installing the Light Shield 1 Open the cell compartment 2 Insert the light shield so the arrow on the light shield points to the left and the orientation tab fits the groove in the compartment opening Figure 7 Figure 7 Light Shield Installed 1 Light Shield Cat No LZV646 15 Installation 3 6 4 Using the Protective Cover for Field Measurements The Protective Cover Figure 8 prevents bright light and rain from interfering with sample measurements Use the Protective Cover when both of the following conditions occur 1 Tall cells multi path or 25 mL sample cells are used in Cell Compartment 2 these cells prevent the sliding door fro
56. list of available evaluation forms appears PO P PO4 P total POs Note The selected chemical form is displayed but does not become PO total the default To change the default form see section 5 5 3 1 2 Press the required chemical form and confirm with OK P205 P20 total conc ox i 5 5 3 1 Changing the Chemical Form Default Setting 1 Insert the sample cell or blank depending on the working procedure into the cell compartment 40 Standard Operations In the result display press OPTIONS gt MORE gt CHEMICAL FORM 3 A list of available evaluation forms appears Select the default setting Press OK to confirm 4 Press EDIT and then SAVE The current result and all further measurements will be displayed in the new chemical form 5 5 4 Setting Basic Test and Sample Data Settings 1 Press OPTIONS to change test or sample specific settings 348 Phosphate Dilution Data Factor Off 1 57 m e reme PO P Save as User Program 29 JUN 2005 11 44 13 Main w Instrument Menu ero Options Setup Table 4 Barcode Program Options Options Description With the Store On setting all measurement data are stored automatically With the Store Off Store Off On setting no measurement data are stored Abs Trans Switches to transmittance or absorbance readings Send Data icon Sends data to a printer computer or USB memory stick
57. lug onto the supplied external power supply Figure 1 Plug the external power supply cord into the connector on the back panel of the instrument then plug the supply into a power outlet 100 240 V 50 60 Hz Press the power switch on the back of the instrument to initialize power Figure 2 on page 11 Figure 1 Power Adapter 3 Adapter Plug Lock Release Button 2 Power Supply 4 Power Supply with Adapter Plug Installed 3 4 Battery Power The DR 2800 can also be powered using an optional lithium battery pack Refer to section 7 2 on page 74 for more information on battery replacement and charging 3 5 Serial Printer and Personal Computer PC Connections The DR 2800 has two USB interfaces as a standard feature located on the back of the instrument Figure 2 The USB Type A interface is used for communications with a printer USB memory stick or keyboard A USB memory stick is used to update instrument software section 6 6 2 on page 68 The USB Type B interface is used for communications with a PC The optional Hach Data Trans software Cat No LZY274 must be installed on the PC for this use Important Note USB cables must not be longer than 3 meters 10 feet Installation Figure 2 Interfaces 1 On Off Switch 4 Cover 2 USB Type B for PC to DR 2800 connection 5 USB Type A for printer USB stick or keyboard 3 External Power Supply Socket 11 Installation 3 6 Cell Compartments Cell Adapter
58. ly switches off Press OK 5 Minutes C 2 Hours 15 Minutes disabled 30 Minutes 1 Hour 5 2 6 Printer and PC Communications 5 2 6 1 Printer Setup Instr nt EUER Note For compatibility the printer language must be HP PCL 3 PC amp Printer Printer Deskjet 6500 1 From Instrument Setup press PC amp PRINTER A list of connection information will be displayed Press PRINTER Press SETUP to display the Printer Setup screen Select the resolution 100 150 300 dpi and paper size letter legal ledger executive A4 Press OK to confirm and press OK again to return to the Instrument Setup menu nstr fee mek ee nm nstr fe ee umi a n Printer Setup Resolution Paper Resolution 100 dpi 150 dpi 26 5 2 6 2 Printing Data Data Log 3 9 JUN O5 07 38 43 ww 01 a 0 339 Abs Single 29 JUN 05 07 38 55 ww 02 0 515 Abs Single AAA Sending Data View lm Details Options ie 0e G9 mn Standard Operations From the Main Menu press RECALL DATA Select the data source where the data to be printed are stored Select the data record that is to be printed from this list Press the PRINTER icon to send the data table graph immediately to the printer Activate Single point Filtered data or All data and press OK Sending Data is displayed until the data have been printed 5 2 7 Setting Security Options Password Protection Instr eee ee ee a Pas
59. m closing AND 2 The instrument is used outdoors in bright sunlight Most other conditions do not require use of the Protective Cover however in some lighting conditions the error message Too much ambient light Move instrument into shade or close the lid may appear If this warning appears either close the lid move the instrument away from direct sunlight or place the Protective Cover over both sample compartments Figure 9 The Protective Cover can also be used in rainy weather to prevent water from entering the cell compartments The enclosure rating of the instrument increases from IP41 to IP42 when the Protective Cover is in place The inside of the Protective Cover can be used for storage of three cell adapters Figure 8 The cell adapter recesses in the Protective Cover are marked with the corresponding letters and arrows of the adapter The arrows indicate the direction of insertion When a cell adapter is in use and removed from the cover the Light Shield can be stored in the Protective Cover Figure 8 Protective Cover 1 Protective Cover Cat No LZV642 3 Protective Cover with adapter A stored in position A 2 Protective Cover inside view Positioning the Protective Cover 1 After the sample is placed in the cell compartment position the Protective Cover over both cell compartments The lettering Protective Cover must be readable from the display side of the instrument Figure 9 16 Instal
60. metric analysis The interference factors come from the mg l sample or are created by reactions with the reagents 0 1 2 O Cr The influence of turbidity and or color can be eliminated or reduced by taking a sample blank reading In the barcode mode a special cell LCW919 containing the sample blank is placed in the round cell compartment after the E sample reading has been taken and is automatically measured Z8 APR 2005 11 52 56 e The sample reading is then corrected by adding or subtracting the blank value and the final result is displayed together with the message After blank value corr Some barcode tests do not require a sample blank value to be determined as turbidity and color are dealt with during the test procedure 5 5 6 Updating a Barcode Test 5 5 6 1 Manually Updating a Barcode Test Using the data provided in the barcode the instrument automatically sets the measurement wavelength and factors If a discrepancy is detected between the barcode data and the stored data or a new test is identified the instrument requests an update Barcodes controinumber Data types are described in Table 5 Instructions for editing values are in the sections following Table 5 Please update program data Table 5 Program Update Options Name Name of the analysis parameter An abbreviation assigned by the user or the version number etc is entered here Exact definition of the test the number of wavelengths at whic
61. mula have been entered The wavelengths must be in the range from 340 900 nm Press OK Concentration Factor Kx Multi wavelength Multiplication factor for converting absorbance values into concentration values Advanced Operations Press a factor key and use the alphanumeric keypad to enter a factor If the formula includes more than one factor press another factor key and enter another factor and repeat until all the factors have been entered Press OK When all the relevant data have been entered press NEXT Note Up to five digits can be entered including a maximum of 4 decimal places to the right of the decimal 277 aaa Concentration Resolution Single and Multi wavelength Concentration Resolution Select the required number of decimal places from the displayed list and press NEXT ET Chemical Form Single and Multi wavelength Chemical Form Chemical representation of the analysis parameter in the cn result display do Use the alphanumeric keypad to enter the chemical form and press MEM ee DEF GHI A NEXT to enter Calibration settings see section 6 1 1 3 on page 51 90 JKL MNO Par 5 123 stu vwx vi 6 1 1 3 Calibration Settings for Single and Multi Wavelength Mode A method is calibrated by determining the absorbance values of several standard solutions of known concentration There are three ways to create and store a calibration curve Instructi
62. n of written procedures often suggests a standard solution concentration for this purpose Head a standard before setting Standard Adjust to On 1 Follow the entire procedure using a known standard for the sample 2 After reading the concentration press OPTIONS MORE STANDARD ADJUST 3 If Standard Adjust is set to Off turn it On The Current Reading will show the concentration The box on the right will show the suggested default standard value for the test as mentioned in the procedure 4 Ifthe measurement used a standard concentration that is different from the one displayed in the box press the box on the right to enter a different standard value and enter the new value Press OK to confirm 5 Press ADJUST to enable the Standard Adjust The STANDARD ADJUST icon will appear Note The adjustment must be within certain limits which vary with each program The allowable percentage is shown after Adjustment Note When a Standard Adjust is in effect the STANDARD ADJUST icon will appear on the display oome Stored Programs allow a variety of chemical forms to be selected Press the unit e g mg L or the chemical representation of the evaluation form e g Ale A list of available chemical forms is displayed Select the required form by pressing the corresponding entry in the list Note When the program is exited the evaluation form reverts to the standard setting Note The stoichiometric conversion o
63. ncentration 50 00 mg L Al 58 Optic Standard Additions Standard Additions mL mg l Standard Standard Additions Approx Cone O 305 mg Lal 0 151 ma L Curve Fit r 1 0000 r 10 11 Advanced Operations Press the keys to enter the standard addition volumes Use the alphanumeric keypad to enter the new data and press OK A table of the reading is displayed e The first column shows the standard addition volume O0 mL stands for a sample containing no added standard e he second column shows the reading of the samples with and without added standard e The third column shows the detection rate of the added standard e The black highlighted line is active The reading of the sample in the instrument without added standard is automatically shown under 0 mL Prepare the standard addition solution as described in the procedure Use the arrow keys to select the first standard addition volume in the table and place the cell with the corresponding volume of added standard in the cell compartment Press READ Hepeat the procedure from step 9 with all the other standard addition solutions 12 13 After all the standard addition solutions have been measured press GRAPH The regression line through the standard addition data points is displayed The correlation coefficient r indicates how close the data points are to the line If the correlation coefficient 2 1 the cur
64. nd keyboard Safety Class Safety class Il Visit us at www hach com Section 2 General Information 2 1 Safety Information 2 1 1 Use of Hazard Information 2 1 2 Precautionary Labels Please read this entire manual before unpacking setting up or operating this equipment Pay attention to all danger and caution statements Failure to do so could result in serious injury to the operator or damage to the equipment To ensure that the protection provided by this equipment is not impaired do not use or install this equipment in any manner other than that specified in this manual In addition to the instructions in this manual users must comply with the national general safety and accident prevention regulations of the country in which the instrument is used DANGER Indicates a potentially or imminently hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a potentially hazardous situation that may result in minor or moderate injury Important Note Information that requires special emphasis Note Information that supplements points in the main text Read all labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A symbol if noted on the instrument will be included with a danger or caution statement in the manual This symbol if noted on the instrument references the instruction manual for operation and o
65. nter the standard concentration Press OK C Enter Values Read Standards C Enter Formula 3 Press the symbol again and enter the next standard concentration Repeat this sequence until all standard concentrations maximum of 24 solutions have been entered 4 Highlight the appropriate concentration line for the first standard and insert the cell with the corresponding standard solution 5 Highlight the zero concentration line Place the zero solution in the cell compartment Close the compartment Press ZERO 6 Highlight the appropriate concentration line for the first standard and insert the cell with the corresponding standard solution Press READ 7 Highlight the second standard line Place the second standard solution in the cell compartment Press READ Repeat this sequence until all the standard solutions have been measured maximum of 24 solutions The entered and measured data are displayed in the table Note To delete a standard concentration select the appropriate line and press the Delete icon 8 When the data have all been entered and the measurements have all been performed press GRAPH to display the curve that results from plotting the entered data 9 The linear equation corresponds to the standard setting Press NEXT CURVE to display the polynomial 2nd order curve Press NEXT CURVE again to display the polynomial 3rd order curve 10 Press FORCE 0 to change the s
66. o each selected measurement point of the scan Curve Icon Delta mode A second cursor is activated The position of the fixed cursor was previously defined in Cursor Mode Single Use the active cursor to select any point on the measurement curve The difference in time between the two cursors The delta values are correspondingly highlighted and displayed on the x and y axes The slope of the curve and the square of the correlation coefficient r2 between the cursor points in the Delta mode are shown under the curve The right and left arrow keys are used to move the cursor depending on the selected mode to the next data point The data of the point wavelength absorbance or transmittance value are highlighted on the x and y axes Press any point on the curve to display the associated data Arrow Keys This function is used to magnify a section of the curve The original curve size can be restored by pressing the ZOOM icon again 6 6 System Check Main Menu W 1 From the Main Menu press SYSTEM CHECKS Stored Barcode Programs Programs User Favorite Programs Programs Single Wavelength System Instrument Checks u The System Check menu contains instrument information and Frege ers ae various performance tests Information Update Optical Checks Output Checks Lamp History Factory Service 67 Advanced Operations 6 6 1 Instrument Information M str Pa ud rS s y Instrument Informat
67. omatically switches off the battery can be used for 7 days before it needs recharging The battery has a limited lifespan The more it is used the more its capacity is reduced If a fully charged battery only allows the instrument to be used for a relatively short time replace the battery with a new one Note The battery does not need to run down completely before recharging Recharging the battery with a residual charge does not reduce capacity 75 Maintenance 7 3 Lamp Replacement 76 The source lamp operates at high temperatures To avoid a possible electric shock disconnect the instrument from the power source before servicing the lamp Make sure that the instrument cannot be accidentally switched on again To prevent a possible burn allow the lamp to cool at least 30 minutes before handling CAUTION If the instrument has been in use wait until the lamp cools down Contact with the hot lamp can cause burns Remove the cell from the cell compartment if necessary Switch the instrument off Unplug the power cord Turn the instrument over carefully and place it on a soft surface Use a screwdriver to remove the screw from the cover Remove the cover Remove the battery if installed Figure 11 on page 75 ean OG M S o YS Use a Phillips screwdriver to remove the screws from the lamp housing Figure 12 on page 77 9 Carefully lift the lamp housing from the instrument 10 Carefully remove both knurled scr
68. on 1 Activate the line containing Version and press EDIT Use the alphanumeric keypad to enter the name indicated in the working procedure 2 Press OK to confirm Enter the Measurement Process For detailed information on entering the measurement process see section B 1 on page 91 Check the working procedure in advance to determine whether this point needs to be changed 1 Activate the Measurement Process line in the data overview and press EDIT 2 Press EDIT select the sequence to be edited and press DELETE 3 Press NEW and use the alphanumeric keypad to enter the process indicated in the working procedure Enter the Formula Concentration Units Designation Measuring Ranges For detailed information on entering a Formula see section B 2 on page 93 Check the working procedure in advance to determine whether this point needs to be changed 1 Activate the Formula line in the data overview and press EDIT 2 Select the formula to be edited press EDIT and use the alphanumeric keypad to enter the data specified in the working procedure for C12 C2 units name measuring range limits etc 43 Standard Operations Prog ZA 47 Yariables F1 5 0380 Fe 0 4080 Timers Dimer DO Timer 00 00 Timer Enter the Variables Factors Wavelength and Conversion Factors For detailed information on entering Variables see Appendix B Free Programming on page 91 Check th
69. ons for each method follows Table 8 Table 8 Calibration Settings e IM o oo uL A calibration table is created by entering the concentration values and the absorbance values of the Enter Values analyte solution The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph page 52 A calibration table is created by entering the concentration values of the standard solutions and then Read Standards measuring the absorbance of the analyte solutions The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph page 53 If the calibration curve can be determined from the mathematical relationship between concentration and Enter Formula absorbance by linear regression etc the corresponding formula can be selected linear 2nd or 3rd order polynomial from a list and the appropriate factors can be entered page 54 51 Advanced Operations ug er Res Se See Calibration Enter Values Read Standards C Enter Formula Calibration Values 1 000 Abs 5 00 mg L Curve Fit r 1 0000 Calibration Values C a b c 1 000 Abs 5 00 mg L Curve Fit r 1 0000 52 Calibration by Entering Calibration Values Select the Enter Values and press NEXT To enter the standard concentrations and corresponding absorbance values in the displayed table press the symbol
70. ored Toggles between 96 transmittance absorbance or concentration readings Absorbance Measures the amount of light absorbed by the sample in units of Absorbance 95 Trans Abs Conc Transmittance Measures the percent of the original light that passes through the sample and reaches the detector Concentration Allows the selection of a specific multiplier for converting absorbance readings to concentration X Wavelength Enter the measurement wavelengths Use the alohanumeric keypad to enter the measurement 9 wavelengths The entered wavelengths must be in the range from 340 900 nm Functions as a stopwatch It helps to ensure that the steps of an analysis are correctly timed e g Timer icon reaction times wait times etc can be exactly specified When the specified time has elapsed an audible signal is emitted The use of the timer has no influence on the measurement program Concentration Factor Multiplication factor for converting absorbance values into concentration values Concentration Resolution Select the position of the decimal point in the calculated concentration readings 62 Unit hy Advanced Operations Absorbance Formula 1 Press ABSORBANCE FORMULA 2 The formula selected in the top key determines the number of wavelength and coefficient keys that will appear below To change the absorbance formula press the top key select a formula from the displayed list and press OK Wh
71. orking procedures of the corresponding test contains the new data Wavelength Factors Measurement Ranges Conversion Factor for chemical Form etc 5 Highlight the corresponding line and press EDIT When finished programming press STORE and then CANCEL to return to the User Programs menu T Press START to start the test with the new data Standard Operations Option 2 Manual Check Revision of Test Data ss Dilution If an incorrect entry was made during the data update and is not Factor Of recognized via the barcode of the cell e g an incorrect measuring Chemical Form range another option is available for checking and if necessary POs P revising the test data User Program Instrument Setup 5 5 6 3 Programming a New Barcode Test Main te 1 2 Program not available 3 Please update program data Program 342 4 SSS el Marne Mitrit 5 6 Cancel 7 Insert the Light Shield in Cell Compartment 2 The instrument needs to be at the Main Menu or press BARCODE PROGRAMS in the Main Menu Insert the sample cell or blank depending on the working procedure into the cell compartment Press OPTIONS gt MORE gt EDIT A data overview is displayed including the specifications of the test that is to be revised Compare the displayed data with the data in the working procedure and edit the displayed data in accordance with the working procedure data Insert the Light Shield in
72. portant Note Make sure that the battery contacts are always clean Soiled contacts can result in contact heating and voltage drop interfering with the proper operation of the spectrophotometer 10 Use a screwdriver to replace the screw securing the cover 11 Carefully stand the instrument upright 12 Plug in the power supply The Instrument is now ready for use The instrument can be operated using line power while the battery charges Maintenance 7 2 1 Charging the Battery 7 2 2 Battery Life Figure 11 Battery Replacement 2 Battery 4 Instrument Contacts CAUTION Use only the manufacturer s specified external power supply to operate the instrument and charge the internal lithium battery pack The specified power supply and its plug shape voltage rating 12V 2A and electromagnetic characteristics are critical to ensure DR 2800 performance and the safety of the user Important Note Optimal ambient temperature for charging the lithium battery to 100 is 10 30 50 86 F The battery is charged automatically when the plug in power supply is connected and is plugged into a mains socket 100 240 Volt 50 60 Hz The time required to charge a newly installed battery is about 3 5 hours Operating time of a fully charged battery If a battery is charged to 10096 capacity and the user carries out 10 measurements per day and the instrument is left on after each measurement for the full 15 minutes before it aut
73. r safety information Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after 12 August of 2005 In conformity with European local and national regulations EU Directive 2002 96 EC European electrical equipment users must now return old or end of life equipment to the Producer for disposal at no charge to the user Note For return for recycling please contact the equipment producer or supplier for instructions on how to return end of life equipment producer supplied electrical accessories and all auxiliary items for proper disposal This symbol if noted on the product indicates the need for protective eye wear General Information 2 1 3 Class 1 LASER A Class 1 LASER is installed in this instrument Class 1 LASERS are products where the radiant power of the LASER beam accessible the accessible emission is always below the Maximum Permissible Exposure value Therefore for Class 1 LASERS the output power is below the level at which it is believed eye damage will occur Exposure to the beam of a Class 1 LASER will not result in eye injury Class 1 LASERS may therefore be considered safe However Class 1 LASER products may contain LASER systems of a higher Class but there are adequate engineering control measures to ensure that access to the beam is not reasonably likely Examples of such products include LASER printers and compact disc players CDRH assession number 0510555 01
74. rcode Programs ZH 3 Insert the sample cell or blank depending on the working procedure into the cell compartment The measurement process and the result are displayed automatically Please insert barcode cuvette To evaluate other TNTplus cell tests and other parameters place the prepared cell in the cell compartment and read the result 29 JUN 2005 11 40 52 Main Menu 5 5 2 Selecting the Measuring Range Some tests can be used for different measuring ranges After the sample cell has been inserted a list of the different measuring 326 Magnesium ranges is displayed Stored Programs 326 Magnesium Select the measuring range by pressing the appropriate line Press START PERMANENT if this measuring range is to apply to all subsequent measurements Changing the standard setting In the result display press OPTIONS gt MORE gt PERMANENT ON The key now reads Permanent Off Selectbv Number 5 5 3 Selecting the Chemical Evaluation Form The chemical form of the test result of a number of parameters can be selected individually 1 In the result display press the unit e g mg L or the chemical representation of the evaluation form e g PO4 P A list of possible evaluation forms is displayed from which the required form can be selected Press OK to confirm Another way of changing the standard setting is 1 Inthe result display press OPTIONS gt MORE gt CHEMICAL FORM Chemical Form A
75. rization must be obtained from Hach Company before sending any items for repair Please contact the Hach Service Center serving your location In the United States Hach Company Ames Service 100 Dayton Avenue Ames lowa 50010 800 227 4224 U S A only FAX 515 232 3835 In Canada Hach Sales amp Service Canada Lid 1313 Border Street Unit 34 Winnipeg Manitoba R3H 0X4 800 665 7635 Canada only Telephone 204 632 5598 FAX 204 694 5134 E mail canada hach com In Latin America the Caribbean the Far East Indian Subcontinent Africa Europe or the Middle East Hach Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Telephone 970 669 3050 FAX 970 669 2932 E mail intt hach com Section 12 Limited Warranty Hach Company warrants its products to the original purchaser against any defects that are due to faulty material or workmanship for a period of one year from date of shipment unless otherwise noted in the product manual In the event that a defect is discovered during the warranty period Hach Company agrees that at its option it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges Any product repaired or replaced under this warranty will be warranted only for the remainder of the original product warranty period This warranty does not apply to consumable products such as chemical reagents or
76. s e Creation of a specific selection of methods and tests 1 From the Main Menu press USER PROGRAMS and then PROGRAM OPTIONS Program Options The Program Options menu contains several input and editing np say Program Options options Table 6 Table 6 Program Options Option Description oooo Select NEW to program a new user program The first time PROGRAM OPTIONS is selected only the NEW i option is available The other options remain inactive gray until the first program has been created Add to Favorites Select ADD TO FAVORITES to add an existing user program to the list of frequently used programs Select EDIT to modify an existing user program Select DELETE to remove a program from the list of user programs The program will be simultaneously Delete l deleted from the Favorites list 47 Advanced Operations 6 1 1 Programming a New User Method All input steps and their significance and options are explained in the following sections 1 From Program Options select New jenem Program Number Programi PMumber SO 295 Specific test number with which the program can subsequently be called up from the selection list in the User Programs menu or the Favorites menu 2 Use the alphanumeric keypad to enter a program number between 950 and 999 The lowest available number appears automatically 3 Press OK Note If the program num
77. s Light Shield and Protective Cover 3 6 1 Cell Compartments and Adapters The DR 2800 has two cell compartments Figure 3 Cell Compartment 2 uses adapters Figure 4 to accommodate different cell types Only one cell type at a time can be used fora measurement Cell Compartment 1 e 13 mm TNTplus or 16 mm TNT round cells Note Cell Compartment 1 contains a barcode reader for TNTplus vials Cell Compartment 2 e 1 inch square or 50 mm rectangular cells can be inserted directly into the cell compartment without using an adapter e Adapter A 10 mm square cells e Adapter B Pour Thru and multi path cells Note Pour Thru cell must be used with Adapter B not Adapter C e Adapter C One inch round cells and AccuVac Ampuls Note One inch round cells and AccuVac Ampuls must be used with Adapter C not Adapter B Figure 3 Cell Compartments 1 Cell Compartment 1 2 Cell Compartment 2 12 Installation Figure 4 Cell Adapters 1 Adapter A Cat No LZV583 10 mm square cell adapter 2 Adapter B Cat No LZV585 1 inch Pour Thru and multi path adapter 3 Adapter C Cat No LZV584 1 inch round cell adapter 3 6 2 Installing Cell Adapters 1 Open the cell compartment select the correct adapter for the cell type Insert the adapter so the arrow on top of the adapter points to the left and the orientation tab fits the groove in the compartment opening The cell type imprint shou
78. s 12 9 5 2 Installing Gell Adapters xiatstesicissihEi cia pa est eon SH leas nde ie ated ee eee he 13 3 6 3 Using the Light Shield for TNTplus or Test N Tube Measurements 15 3 6 4 Using the Protective Cover for Field Measurements cccsecccceececeeeeeceeeeseeeeeaeeeeeaaees 16 SECHNA oa o o dem TM 19 4 1 Powering the Instrument On and Off 2 0 ecccccccseeeceeceeeeeeeseeeeeeeeseeeeeeeseeaeeeeeseaaeeeeeseaaeeessnaaes 19 AZ Language Selecion aiii uctus toam lin statt deel pte ea E e MM CM ALL t D DI dc uty 19 4 9 oystemdIagriOSliCS aneirin a sce a aU sas e ER MEER MI MM R 19 Section 5 Standard Operations Etre es De a Eu aces ios e M sot bs OM o asm uM 21 SM GENSAN U e CTI E 21 541 Mips tor Using the POUCH SCIECN acsee e e A 21 5 1 2 Using the alphanuimeric Keypad Jo seed eeu idus tunc e idee pa ti Ged Moon a Eua ieee 21 5o Main MENU m L 22 5 2 Isidis ziMwi qsemjee mL 23 5 2 Setting the ODeraltor ID uc eres ede etude 10 E Fee a sate cetiaeeeedteeetig 23 62 2 Setting INE Sample ID em 24 0 2 3 Seting the Dale ang TIME 299 cede iar teenie acl eben uelit be atuanual da pd 25 5 2 4 Setting the Display and Sound Preferences ccccecccccseeeeceeeeeceeeeeesseeeesaeeseesaeeessaaeees 25 5 2 5 Setting the Power Management Options cccccceeeecceceeeeececeeeeesee
79. s OK to confirm The display shows the chosen Operator ID Press OK The instrument will return to Instrument Setup and show the selected operator identifier 7 Operator ID is activated Note To remove an Operator ID highlight the ID and press DELETE Note Alternatively an Operator ID can be entered or changed in measurement mode In the results screen press OPTIONS gt MORE gt INSTRUMENT SETUP If an Operator ID is already assigned select the Operator ID symbol in the results screen 23 Standard Operations 5 2 2 Setting the Sample ID Instr E 1 T1 E ee TEES Sample ID Instr fee ee PII Sample ID HE abc ABc DEF ani CE EN Boo faa ln lt Off gt WASTE WATER Number x TE A New Delete OK 24 Use this option to enter up to 30 Sample Identification tags up to 13 characters each into the instrument Sample IDs can be used to specify the sample location or other sample specific information 1 From Instrument Setup press SAMPLE ID 2 Press NEW to enter a new Sample ID 3 Use the alphanumeric keypad to enter a new Sample ID Note Spaces cannot be used in this function Use underscore symbols instead on the key YZ 4 Press OK to confirm 5 Tonumber the Sample IDs sequentially e g Inflow 01 etc select Add Number e Use the arrow keys to specify the first number of the sequence e Use the key between the arrow keys to enter the first number
80. s often as specified in the procedure Refer to the instruction sheet included with the Pour Thru Cell for more information Pour Thru Cell A 4 Using the Pour Thru Cell The Pour Thru Cell is intended primarily for those methods that specifically call for its use These use liquid reagents and are typically denoted as ULR or Rapid Liquid methods These methods obtain maximum benefit from the Pour Thru Cell by avoiding turbidity from un dissolved reagents and provide the precision and sensitivity required to determine very low concentrations These methods require no modification when the Pour Thru Cell is used It is possible to use the Pour Thru Cell for other methods with a few minor modifications The cell requires a minimum volume of 25 mL for proper flushing of any previous solution from the cell so methods that utilize a 10 mL sample volume cannot be used directly Tripling the sample and reagent volumes on these methods or use of 25 mL reagents and sample volumes will provide enough liquid to use the cell however an additional modification is needed Since the Pour Thru Cell has a slightly shorter 1 inch pathlength than the square cells for which most methods are calibrated it is necessary to multiply the results by a pathlength factor of 0 95 or to perform a standard adjustment section 5 4 5 on page 36 on any method that utilizes 1 inch square cells Always confirm results with a standard solution to make sure modifica
81. seeeeseeseaeeeeeeseeaeeeeeeas 25 5 26 Printer and PG GommMuUniCalOnS sospes ek ai gh pdbae eoe epu ebria but uet YEN epe oin itp Sa Nea mendo 26 5 2 7 Setting Security Options Password Protection cccccsseeceeceeeeeeeeeeeeeeesaeseeeeesaeeeeeeseas 27 5 3 Storing Recalling Sending and Deleting Data esseseeeeeeeerreeeeee 28 591 The DAA LOG Nc 28 D32 MME COUSO dcs atat aei a a a e sy ccu he NIS EAD Eta EU LURUf 30 DA Stored Progam S usr a utentes E tes ette O csset a ee 33 54 1 Selecung a Stored Progra sssrinds e A N 33 54 2 Stored Program ODUONS dinee a a a 33 5ko WISIING Prograin TIMES sparse aa A E T 34 544 Setmng the Bleue T 35 0 429 Bunripdg a Standard AJUS t iriran a uie MeL Ee eut 36 54 6 Set ng the Chemical EON oia tm o oe T eedecccussncs Eus equ Ono estat 36 5 2 7 RUMIN a Heagent BANK esa cidusviede dni ue Maven ice oannes dude eva undated sudo ce Eu e 37 548 Analysis OSAMDESET E 38 5 4 9 Adding Stored Programs to the Favorite Programs List seeeseeeeessseuss 38 5 9 Barcode PIOGLAITIS qsesseseniseetatesbupbisueete e ntat iiM iret sede cbu stead dated rude ui set dx aprenden peut eua tune 39 5 5 1 Pertormning a TNTDIUS Tesla eh rdiet QR DR UR es oap S any ested o oe Duae uult e as toe 39 Table of Contents 5 5 2 Selecting the Measuring Range sersem ene a aE aeRO nnns 40 5 5 3 Selecting the Chemical Evaluation Form
82. sible press the icon and select GENERAL TIMER A new screen will appear Enter the length of the timed interval and press OK to start the timer The timer will beep when the timed interval ends The Dilution Factor function is used to calculate the original concentration of a sample that has been diluted by a known ratio For example dilution factor can bring the analyzed concentration within the test range 1 Press OPTIONS gt MORE gt DILUTION FACTOR The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment and display the concentration of the original undiluted sample For example if the sample has been diluted by a factor of 2 enter 2 The default setting of the dilution factor is 1 corresponding to no dilution 2 Press OK to confirm Press OK again Note When a dilution is in effect the dilution icon will appear on the display Note If using undiluted samples set the dilution factor to 1 again 35 Standard Operations 5 4 5 Running a Standard Adjust Standard Adjust Current Reading 0 131 Adjust to 0 111 mg L Al cancel Read Adjust 10 Aluminum Alumin 522 nm 0 111 29 JUN 2005 08 19 55 Q ilh Options Optic EU Ghemical Form 36 The Standard Adjust functions allows the calibration curve for a stored program to be adjusted based on analysis of a known standard solution The Accuracy Check sectio
83. specific multiplier for converting absorbance readings to concentration Functions as a stopwatch Ensures that the steps of an analysis are correctly timed e g reaction Timer icon times wait times etc can be exactly specified When the specified time has elapsed an audible signal is emitted The timer has no influence on the measurement program Multiplication factor for converting absorbance values into concentration values In a graph of Concentration Factor concentration versus the absorbance the concentration factor is the slope of the line Concentration Resolution Select the position of the decimal point in the calculated concentration readings Save as User Program Stores the selected parameters as a User Program section 6 1 on page 47 Recall Data Recalls saved measurement data wavelength scans or time courses section 5 3 on page 28 Instrument Setup Basic operation settings of the instrument section 5 2 on page 23 X Wavelength Enters the measurement wavelength Use the alphanumeric keypad to enter the measurement 9 wavelength The entered wavelength must be in the range from 340 900 nm 60 Advanced Operations Setting the Concentration Factor 1 From the Options menu press CONCENTRATION FACTOR OFF Select On to activate this feature Optic Concentration Factor 2 Press the FACTOR key and use the alphanumeric keypad to enter the factor by which absorbance readings are to be multiplied Press the
84. statement of warranty terms and no person is authorized to make any other warranties or representations on behalf of Hach Company Limitation of Remedies The remedies of repair replacement or refund of purchase price as stated above are the exclusive remedies for the breach of this warranty On the basis of strict liability or under any other legal theory in no event shall Hach Company be liable for any incidental or consequential damages of any kind for breach of warranty or negligence 85 Visit us at www hach com Appendix A Pour Thru Cell CAUTION Do not use the Pour Thru Cell in tests that call for the use of organic solvents such as alcohol toluene chloroform trichloroethane or cyclohexanone These solvents may not be compatible with the plastic components of the Pour Thru Cell creating the potential for equipment damage and chemical exposure for the analyst The Pour Thru module is an optional accessory for the DR 2800 Spectrophotometer Working with the module improves measurement accuracy as zeroing measurements and measurement comparisons are carried out under the same optical conditions As the samples all flow through the same cell errors attributable to the different optical characteristics of individual cells are excluded The module is designed to allow the sample to be introduced into the cell without having to handle the cell in any way The Pour Thru cell can be oriented to provide two pathlengths e Approxima
85. surement in the Multi Wavelength Mode Multi Wavelength eee 61 Insert the blank cell into the cell holder Press ZERO Note The READ key does not become active until the zero 0 8 7 8 measurement has been performed E ABS 5 Insert the sample cell into the cell holder Press READ haoo Asoo 2527 0251 For data storage see section 5 3 1 on page 28 Options 6 5 Time Course Mode The Time Course Mode is used to collect data in either absorbance or transmittance for a user specified length of time After the data are collected they can be displayed in either graphic or tabular format 6 5 1 Time Course Setup Parameters 1 From the Main Menu press TIME COURSE 2 Press OPTIONS to configure parameters Time Course Options a Send Scale amp Units cH Data Interval 29 3UN 2005 13 14 06 Main v p Instrument 64 Advanced Operations Table 11 Time Course Setup Options Store folder icon Stores the scanned data Time amp Interval Enters the total time for data collection and the time interval between the collection of data points Wavelength Enters the wavelength setting Displays readings in absorbance or transmittance This can be changed after sample data are collected Scale In the automatic scaling mode the y axis is automatically adjusted so that the total range is displayed The manual scaling mode allows sections of the scan to be displayed Units Choice of absorbance or 96 transm
86. sword Security v Off C n m Set Password Security List i Instp I Wt Password The Password menu contains a variety of security settings to control access to various functions From Instrument Setup press PASSWORD select SET PASSWORD to activate the Security List Use the alphanumeric keypad to enter a new Password up to 10 characters and press OK Access to the Security List is activated Press SECURITY LIST to lock various functions for unauthorized users Activate the desired functions to control Press OK to confirm the Security List The Password menu will be displayed Press ON to activate the new settings of the Security List Enter the new Password again to confirm Press OK to return to Instrument Setup Note The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting 27 Standard Operations 5 2 7 1 Deleting or Changing a Password Instr Se ee Password fo PEG B iai Instr DT TIS IIT Password Security 1 From Instrument Setup press PASSWORD 2 Use the alphanumeric keypad to enter the former Password and press OK to confirm 3 Press SET PASSWORD 4 Press OK to deactivate the former Password and return back to the Password menu Set a new password refer to section 5 2 7 on page 27 5 3 Storing Recalling Sending and Deleting Data 5 3 1 The Data Log 5 3 1 1 Auto Manual Data S
87. tallation press any area of the display with the instrument off Turn the instrument on continue to press the display until the list of languages is displayed Each time the instrument is powered up a series of diagnostic tests are performed automatically to ensure operation of major system components This procedure which takes approximately two minutes checks the System lamp filter adjustment wavelength calibration and voltage Each test which functions correctly is confirmed with a check mark The Main Menu is displayed when power up diagnostics are completed 19 Start Up u an If the instrument detects any deviation relative to the last n Full System Check calibration it is recommended to perform a system check 1 Remove the cell from the cell compartment if present Close It s recommended to execute the lid and press START a Full System Check Please remove the cuvette Please close the lid Cancel Sree ONSET The system check is carried out duration approximately y Full System Check 6 minutes Note For more information on error messages during self check see section Section 8 on page 79 Please wait Approx 6 minutes Please keep lid closed oa Cancel 20 Section5 Standard Operations 5 1 Getting Started 5 1 1 Tips for Using the Touch Screen The entire screen is touch activated To make a selection press the screen with a fingernail fingertip pencil
88. tely 1 inch e 1cm These two pathlengths can be used over the entire wavelength spectrum of the DR 2800 Spectrophotometer A 1 Pour Thru Module Specifications Path length Wavelength Range Rinsing Volume A 2 Unpacking Specifications are subject to change without notice 22 mm approximately 1 inch 1 cm 0 394 inch 340 to 900 nm At least 25 mL Remove the Pour Thru module from its shipping carton and inspect it for damage The Pour Thru Module set includes e Pour Thru cell e Stand including mountings for glass funnel and stand pipe e Plastic tube inside diameter 1 8 for the cell inflow and outflow e Stand pipe e Rubber tube Inside diameter 1 4 drain tube for stand pipe e Glass funnel e Instruction sheet If any of these items are missing or damaged contact the manufacturer or a sales representative immediately 87 Pour Thru Cell A 3 Assembling the Pour Thru Module Installing the Pour Thru Cell 1 2 Open the cell compartment Insert the Pour Thru Cell Adapter B in Cell Compartment 2 so that the light path arrow on the adapter points left see Figure 5 on page 14 Note Do not use Adapter C It places the cell too low and does not prevent rotation of the Pour Thru Cell Clean the Pour Thru Cell with a lint free cloth and insert it fully in the cell holder so that the cell is oriented for the necessary pathlength and cannot rotate in the adapter Note For the 1 inch orienta
89. tery Replacement 74 CAUTION Pay special attention to the following information for the Lithium battery pack Do not short circuit the contacts Keep the battery away from fire e Donot expose the battery to temperatures above 60 C such temperatures may occur for example in a car left in the sun or in direct sunlight The performance of the battery decreases with the temperature e Ensure that the battery does not become wet e Take care that the battery is not exposed to impacts blows etc e Donottake the battery apart or modify it in any way CAUTION For quality and safety reasons only the manufacturer s lithium batteries Cat No LZV551 should be used for this instrument Use of non instrument specific battery types may impair the functioning of and or damage the instrument electronics by overloading them or depending on battery types can result in fire or explosion Remove the sample cell from the cell compartment Switch the instrument off Unplug the power cord Turn the instrument over carefully and place it on a soft surface Use a screwdriver to remove the screw from the cover Remove the cover pU OU ODE Ge I9 x Remove the old battery by lifting the end over the cover screw insert and pulling to remove Figure 11 8 Carefully place the new battery into the compartment with the label facing up 9 Pushin the battery so that the instrument contacts plug into the battery socket Im
90. th the control boxes on the left of the display Timer 3 Timer 4 In the next column a selection can be made from a list of names that describe the corresponding work step In the third column the times for each active timer are entered B 1 Measurement Process The measurement process defines the handling and the measurements of the test e At which and how many wavelengths should measurements be performed e How many absorbance measurements must be performed e When should the zero measurement and the sample measurement be performed e Are waiting times necessary between measurements e Should individual program sequences be repeated The elements of a measuring sequence such as zero and sample measurements and the timer s reaction times waiting times etc are individually defined 91 Free Programming B 1 1 Entering a New Element of a Measuring Sequence Important Note Each component of the measurement process User EEUU mm SSEBrILET must be entered in the order in which it will be performed teas ure mie mt 55 1 Highlight the Measurement Process line in the data overview and press EDIT 2 Press EDIT again and then NEW Content and Definition of the Keys Measurement Process Z Key Zeroing 1 Press the Z key to program a zero measurement and press OK 2 Press NEW and then ZEROING Use the alphanumeric keypad to enter the wavelength at which the zero measurement is to be performed and press
91. the alphanumeric keypad to enter the data specified in the working procedure for F1 F2 A1 U1 etc Press OK to confirm each entry Abbreviation of Variables F1 Factor 1 F2 Factor 2 1 Wavelength 1 U1 Conversion Factor 1 for the first chemical form U2 Conversion Factor 2 for additional chemical forms etc Table 17 Free Programming Variable Definitions Variable Definition a Rad K1 K4 Wavelength weighting factors the sum or difference of the absorbance measured at different wavelengths K values can be fractions e g K 0 75 or negative e g K 1 Absorbance as a function of the Reading and Zero values C1 R1 Z1 C2 Concentration as a function of calibration factors and absorbance values The concentration formula is F1 F2 x C14 F3 x C1 FA x C13 x U1 B 4 Saving a Free Programming User Program Press STORE to save the entered data The data can be stored under any data point Measurement sequence Formula Timer etc 96
92. tion the inlet and outlet fittings are parallel to the light path For the 1 cm orientation the inlet and outlet fittings are perpendicular to the light path 4 Connect the cell inflow and outflow tubes to the Pour Thru Cell Note Make sure there are no kinks in tubes to impair functionality and prevent proper bubble removal A 3 1 Installing the Sample Inflow and Outflow 88 Refer to following instructions to install the sample inflow and outflow 1 Connect the Inflow Tube to the fitting at the bottom of the glass funnel and clamp the glass funnel to the stand Connect the Outflow Tube to the top fitting of the stand pipe Connect the Drain Tube black rubber tube to the bottom fitting of the stand tube and clamp the stand pipe to the stand Connect the free end of the rubber tube to an appropriate waste vessel Note The drain tube must flow freely to the drain or waste vessel Shorten tubes to minimize flow restrictions The funnel height and orientation may be adjusted The funnel height determines the speed of sample flow through the cell The higher the funnel the faster the flow To minimize air bubbles adjust the funnel so that it drains completely with the final level of liquid in the tube about 5 cm 2 inches below the tip of the funnel Take instrument readings after the solution has stopped flowing through the cell Always rinse the cell thoroughly with deionized water after each series of tests or a
93. tions have been performed correctly Although the Pour Thru cell can be used for the methods described in Table 13 the cell must be thoroughly purged with distilled water between individual samples Table 13 Methods that Require Additional Purging Aluminum Aluminon Chlorine Dioxide LR Cobalt PAN Copper Porphyrin Hardness Calmagite Manganese LR PAN Nickel PAN Nitrate MR Nitrate HR The Pour Thru Cell can also be used to run Nitrogen Ammonia Nessler Method and TKN chemistries Clean the cell by pouring a few sodium thiosulfate pentahydrate crystals into the cell Rinse out the crystals with deionized water Important Note In general the Pour Thru Cell cannot be used for the methods described in Table 14 Table 14 Methods that Cannot be Used with the Pour Thru Cell Cyanuric Acid Formaldehyde Lead LeadTrak Suspended Solids Sulfate Volatile Acids Surfactants Anionic Detergents 89 Pour Thru Cell A 5 Pour Thru Cell Maintenance 90 Important Note Do not use solvents e g acetone or alcohol to clean the Pour Thru Cell A dilute acid solution can be used for cleaning Rinse thoroughly with deionized water If windows are cloudy or soiled or bubbles form add 50 mL of a detergent solution to the cell and wait for a few minutes to allow it to take effect Purge the cell thoroughly with distilled water Wipe the windows of the cell with a soft cloth only Paper towels and other paper products may scratc
94. torage g Aluminum ECR Abs gt Trans 29 JUN 2005 08 07 28 Main Menu fero 28 The Data Log will store up to 500 readings taken in the following modes Stored Programs Barcode Programs User Programs Favorite Programs Single Wavelength and Multi Wavelength A complete record of the analysis is stored including the Date Time Results Sample ID and Operator ID The data storage parameter indicates whether data are to be stored automatically or manually in which case the user has to decide which data to store 1 From the Options menu press STORE ON OFF e With the Store On setting all measurement data are stored automatically e With the Store Off setting no measurement data are stored However this setting can be changed to Store On in the result display through Configuration The reading currently shown in the display is then stored Note When the instrument memory data log is full the oldest data are automatically deleted allowing the new data to be stored Standard Operations 5 3 1 2 Recalling Stored Data from the Data Log 29 IUN 05 07 35 43 0 339 Abs Single A 29 JUN 05 07 38 55 0 515 Abs Single 9 JUN 05 07 40 43 0 063 u mgl PO eR View Details Reca Fr mi m Filter Settings C pff Parameter zc AIL 1 From the Main Menu press RECALL DATA 2 Press DATA LOG A listing of the stored data is displayed 3 Press FILTER ON OFF 4 The function Filter S
95. urse 560 nm 6 5 3 Analysis of Time Course Data Time Course j 560 am After the parameters have been selected the instrument must be zeroed then the sample can be analyzed 1 Insert the blank cell into the cell holder Press ZERO The blank reading is shown on the display Insert the sample cell into the cell holder Press READ Start collecting time course kinetic data Note During the measurement the ZERO and READ keys change to MARK and STOP Select MARK to mark the next data point collected This mark may indicate a significant event such as the addition of a sample or other reagent The mark is also shown in the table Select STOP to stop taking the sample readings After the data are collected the following manipulations can be done on the graphic data The Time Course Program is complete if the sound is activated the instrument beeps when the readings are done the graph is shown full size the scaling of the x axis occurs automatically the Cursor functions in the vertical navigation bar are activated 6 5 3 1 Navigating a Time Scan Graph or Analysis 66 After a time scan has been performed the time and the absorbance transmittance data are displayed as a curve Advanced Operations The corresponding absorbance and the elapsed time is highlighted where the cursor is positioned on the curve Table 12 Navigating the Time Scan Choice of Cursor Mode Cursor Mode Single The cursor moves t
96. ve is linear The concentration shown above the curve is the estimated concentration of the sample without the added standard Note Press TABLE to display all the data in the table again Press IDEAL LINE to display the relationship between the added standard solutions and the ideal line detection rate 100 59 Advanced Operations 6 3 Single Wavelength Mode The Single Wavelength Mode can be used in three ways For sample measurements at a single wavelength the instrument can be programmed to measure the absorbance transmittance or concentration of the analyte 6 3 1 Setting Up Single Wavelength Mode 1 From the Main Menu press SINGLE WAVELENGTH 2 Press OPTIONS for Parameter Setup Single Wavelength Concentration Concentration Factor Off Resolution 0 01 Save as User Program 29 JUN 2005 12 55 28 Main gt T Instrument Menu ERO Options Setup Table 9 Single Wavelength Setup Options Option Description ooo With STORE ON selected all measurement data are stored automatically With STORE OFF Store Off On selected no measurement data are stored Toggles between transmittance absorbance readings or concentration Absorbance Measures the amount of light absorbed by the sample in units of Absorbance 95 Trans Abs Conc Transmittance Measures the percent of the original light that passes through the sample and reaches the detector Concentration Allows the selection of a

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