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User Manual - SUPERTECH Instruments

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1. condition would happen even the internal software firmware can be destroyed The enough time before the lonFlow Bipolar can be switched on again after switching off if the last screen content not the backlight but the letters disappears completely and 10 seconds more is spent The connectors of the high voltage output section are signed with a black square around them They are that connectors what you should use the most carefully you can see the Security rules above The Applicator Pipette Output and the Reference Connector are the output points of the current generator end stage The polarity what you select in the software is the polarity of the Applicator Pipette Output referring to the Reference Connector The Floating Shield is an optional connector There are equipment versions assembled with and there are versions manufactured without it There is a special internal electrostatic and electromagnetic shield built onto the floating power supply which is running on high frequency If the Floating Shield connector is present on the back side it means that the special shield is connected to this banana connector and not connected to anywhere else so you should connect the shield to the central Ground point or any other Ground potential chosen by you If the Floating Shield connector is not present on the back side it means that the special shield is connected to the internal low frequency Ground point of the equipment
2. and you should not take care of it The most right input is the Start for Processor BNC connector TTL High level is the active state at this input In the Pos tive External Control and in the Negative External Contro functions the Start for Processor signal serves as a direct external control signal to the current generator end stage in logical OR relation with the Start button In the Positive Timer Negative Timer and in the Programmable Alternating functions the Start for Processor input is an alternative source of a START event see its definition above If there is no signal source connected to the Start for Processor input the microcontroller realizes a TTL Low level as an inactive state Functions in the firmware After switching the lonFlow Bipolar on the microprocessor loads the default setup values saved last time and checks that parameter values If the parameters seem to be valid the processor tries to start the function used last time There are enhanced protection features built into the firmware If any malfunction appears the fault is described on the LCD screen to inform the user about the details Operating modes In all operating functions the timing parameters are generated by the built in microcontroller but the selection of the range and the setting of the currents are carried out with the appropriate analogue controls Positive External Control free control capability from an external device such as a
3. ated floating power supply It is designed for such applications where a signal recording task is running simultaneously with another microelectrode which is located close to the electrophoretic electrode The floating power supply is necessary to avoid the hum noise from the sensitive recording amplifiers Specifications The selectable ranges of the output current O 1 uA 0 100 vA 0 10 mA Compliance voltage of the current generator 120 V Worst case error of the output current generator lt 5 The output current can be set independently for the positive and for the negative polarity with scaled 10 turn precision helical potmeters Resolution of the helical potmeters 0 1 True output current indicator there is a floating LCD digital panelmeter with backlight on the front plate It shows the real output current in the percents of the actual range full scale readout is 100 0 Resolution of the true output current indicator 0 1 Worst case error of the true output current indicator lt 5 Worst case error of the timing parameters generated by the built in microcontroller with firmware lt 0 2 Mains supply voltage 115 VAC or 230 VAC It should be specified in the order The actual supply voltage is sealed at the back side of the equipment Tolerance of the mains supply voltage referring to the nominal value 10 Mains frequency 50 60 Hz Internal power supplies mains frequency power supply with iso
4. computer or from the Start push button on the front panel The Start for Processor signal TTL High level at the back plate serves as a direct external control signal to the current generator end stage in logical OR relation with the Start button Negative External Control same as Positive External Control function with opposite polarity of output current Positive Timer Programmable interval timer running in software in the range of 10 sec to 990 sec with 10 sec of resolution The polarity of the output current is positive Negative Timer same as Positive Timer function with opposite polarity of output current Programmable Alternating continuous square wave alternating current is flowing at the output The positive and the negative period in the cycle can be programmed in the range of 1 sec to 59 sec with 1 sec of resolution The positive and the negative current amplitudes can be set independently of each other Warranty We give you full warranty service including rest parts for the period of 3 years by default Longer warranty periods can also be defined and agreed the actual conditions should be discussed before placing the order Detailed technical and the most recent information www super tech eu International technical hotline by phone 36 20 9234 386 Technical hotline by email csaba niedetzky superte ch
5. dy The output wiring are made by the user or by an authorized technician The professional quality and the electric security of the external wiring and the external circuitry are not the responsibility of Supertech Ltd The electrical security aspects of the lonFlow Bipolar are completely under the sole responsibility of the user Front panel controls On the left side there is a 3 position switch You can choose the actual range of current with this switch The numbers written to the three positions are the full scale values of the ranges Next to the range selector switch there are the potmeters signed Negative and Positive They are used to set the values of the current respectively The potmeters can be used to trim the currents in the actual range The full scale value is generated by the current generator end stage when the potmeter shows 10 in the small window and O on the rotary dial Next to the potmeters there is the true output current indicator It is a 7 segment digital LCD panelmeter with backlight This panelmeter is supplied from a dedicated floating power supply part of the internal power supply unit In this way the user always can monitor the real output current independently of any internal or external circumstances The true output current indicator shows the real output current in the percents of the actual range full scale readout is 100 0 The equipment is assembled with a 2 x 16 character green LCD dis
6. g on Start input The two source of the START event can be used together or independently any time they are in logical OR relation Start button is used to start the output sequence in the Positive Timer Negative Timer and in the Programmable Alternating functions see their descriptions later These functions can be interrupted temporarily with Start button and they can be restarted again with it In the Positive External Control and in the Negative External Contro functions the Start button serves as a direct manual control signal to the current generator end stage in logical OR relation with the signal at the Start for Processor input There is a green LED on the front plate signed as Active It shows when the current generator end stage is passing any current to the output Connectors at the back side On the back plate of the lonFlow Bipolar there is the AC input plug at the left side Connect it to the 115 VAC or 230 VAC mains system with the appropriate cable The actual supply voltage is sealed next to the AC input connector NEVER connect the equipment to other supply voltage than the sealed value because in such a case the equipment will be destroyed The mains switch is also located at the left side of the back plate If the lonFlow Bipolar is switched off it is necessary to wait until the power supply looses all its energy It is prohibited to switch the equipment on again without waiting enough otherwise a faulty reset
7. lation voltage security of 4 kV guaranteed by the manufacturer of the mains transformer and an additional internal floating power source with isolation capacitance lt 10 pF Display 2 x 16 characters alphanumeric LCD with bright green backlight Programming three function buttons and a Start push button on the front plate in menu system Automatic internal diagnostic algorithm in the software Flash program memory Non volatile EEPROM memory to store the parameters used last time Dimensions of the instrument 290 x 250 x 90 mm Weight 2 0 kg Security rules lonFlow Bipolar is supplied from the 230 VAC or 115 VAC 50 Hz mains system They are dangerous voltages All general electrical security precautions should be kept in mind during the usage of the equipment The current generator end stage of the lonFlow Bipolar is a very dangerous electronic circuit On one hand a good current source should have a compliance voltage high enough to pass the current through on high impedance output loads On the other hand a high compliance voltage will be a serious danger to the user of the equipment because the output wires are driven by the compliance voltage And the top of all that the output current of this equipment can be adjusted up to 10 mA It is a very dangerous level of current since the human heart can be forced to stop even with 1 or 2 mA The actual compliance voltage is high enough to pass this current through the human bo
8. lonFlow Bipolar electrophoretic equipment IBP 5 series User s Manual SUPERTECH The choice of the different lonFlow models In our choice there are three different electrophoretic iontophoretic ionophoretic systems This manual describes the lonFlow Bipolar iontophoretic equipment Our other electrophoretic products are lonFlow Modular and lonFlow Pro families All of them are up to date easy to use highly reliable microprocessor controlled constructions We manufacture three different ionophoretic families because the three product lines are optimized for different application fields Please read through the appropriate descriptions for the details lonFlow Bipolar Single channel high voltage general purpose electrophoretic equipment for microelectrode research applications lonFlow Modular Multichannel modularly expandable version of lonFlow Bipolar with an additional current compensating channel for multibarrel electrodes lonFlow Pro High voltage electrophoretic equipment for all general human dental and cosmetic applications The following sections of this manual deal with lonFlow Bipolar General description of the lonFlow Bipolar lonFlow Bipolar is a single channel high voltage general purpose electrophoretic equipment for microelectrode research applications lonFlow Bipolar is optimized to apply tracer solutions in tissues and tissue slices Furthermore it is very effective for in vivo and in vitro si
9. play and a user friendly 4 button keypad on its front plate The development strategy of the firmware was to design an easy to use user interface while highly professional capabilities are activated behind the simple menu system On the front panel of the lonFlow Bipolar there are only four push buttons to program the parameters and control the equipment but with them you can do nearly all what you would like to do Up and Down buttons Up and Down button is used to navigate in menus and to modify parameter values up or down respectively Use them to choose from the menu items and configure the values Where you see the cursor you can adjust the appropriate menu point or you can set that numeric value what is under the cursor F Function button F button has got two functions according to the actual situation Use it in the menu as enter during the programming and parameter setup actions When you are in a programming phase and the Function button works as enter the microprocessor automatically saves the newly selected value if the numeric parameter is valid after the keypress Furthermore Function button is used to cancel a function if the function is running actually When a function is running the F button can be used as escape to inactivate the function to leave the actual task completely and to jump to the Function Choice menu Start button START event means a keypress on Start button or a TTL rising edge appearin
10. tuations and for single cell experiments lonFlow Bipolar can be used efficiently to apply tracers drugs and any other solutions from micropipettes The electrophoresis which collective word covers the effects of ionophoresis and electro osmosis offers a comfortable opportunity to pass medicines and or other chemical substances into any conductive organs e g the human body through the surface of the skin or directly into tissue cultures in correctly measured small quantities with electric current During ionophoresis the ions move due to an electric potential field The non ionic substances can be passed similarly with electro osmosis The substances which are designed in the pharmaceutical factories for human electrophoretic purposes meet these requirement The solutions used in research labs should be designed according to these facts The quantity of substances passed from the electrode can be controlled exactly by two parameters the current and time of the electrophoresis Our electrophoretic equipments offer high accuracy due to their microprocessor based fully digital internal electronic design Since we use state of the art electronic components and extremely thorough quality checking during the manufacturing process the reliability of our products is delicious lonFlow Bipolar has got menu driven internal software firmware so it is very easy to learn how to use it The power supply of lonFlow Bipolar is a highly sophistic

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