User Manual - Leica Microsystems
Contents
1. Parfocal means that a specimen conti i nues to remain exactly in focus even if the magnification on the stereomicroscope is modified All stereomicroscopes from Leica Microsystems are parfocally matched Howe ver the parfocality requires a personal dioptric correction for the user Leica S Series Dioptric Correction In order to parfocally match the stereomic roscope at least one eyepiece with dioptric correction is necessary The setup is described on the following pages With one adjustable and one fixed eyepi ece from page 37 With two adjustable eyepieces from page 40 If you do not wear glasses Depending on the preferences of the observer eyecups can be used To avoid eye infections we recommend A that every user uses his or her own pair of eyecups User Manual If you wear glasses Eyeglass wearers must remove or fold back the eyecups Fig below left as otherwise they cannot see the entire field of view The correct interpupillary distance i The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen If you are still a novice microscope user you may need a short time to become accustomed to this Not to worry after a little while it will become automatic Reference value The interpupillary distance can be set between 55 and 75 mm An exit pupil distance is the distance between eye and eyepiece With the 10x 22. Responsibilities of person in charge of instrument Ensure that the Leica stereomicroscope is operated maintained and repaired by authorized and trained personnel only Important Safety Notes User Manual The individual modules of the Leica stereomi croscope include an interactive CD ROM with all relevant user manuals in several languages Keep it in a safe place and readily accessible to the user User manuals and updates are also available for you to download and print from our web site www leica microsystems com This User Manual describes the special func tions of the Leica StereoZoom stereomicro scopes S series and contains important inst ructions for their operational safety maintenance and accessories The Safety Concept booklet contains additi onal safety information regarding the service work requirements and the handling of the stereomicroscope accessories and electrical accessories as well as general safety instruc tions You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the user manual and the safety requirements of the supplier Before installing operating or using the instru ments read the user manuals listed above In particular please observe all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings con
3. 150 4 lt gt User Manual Leica S Series Leica S6 D with incident light and transmitted light illumination 8 fs Si 207 x ae U 5 a 5 2 di t 00 sp o 076 iN 1 8 ad Y 107 205 rn 205 220 User Manual Leica S Series dent light and transmitted light illumination INCI S8 APO with i ica Le T 107 las 278 107 pa 278 dd 6 o N D Leica S Series Technical data Overview of technical features StereoZoom Zoom Enlargement Viewing angle Extras Leica S4E 4 8 1 6 3x 30x 38 ErgoObjective Leica S6 E 6 3 1 6 3x 40x 38 ErgoObjective Leica S6 6 3 1 6 3x 40x 60 ErgoObjective Leica S6T 6 3 1 6 3x 40x 38 Terminator ErgoObjective Video photo tube Leica S6 D 6 3 1 6 3x 40x 38 ErgoObjective Apochromatic Greenough system Leica S8 APO 8 1 10x 80x 38 Apochromatic zoom Apochromatic objectives Video photo tube StereoZoom is a trademark registered in the Principal Register of the US Patent and Trademark Office Leica S Series User Manual Technical data StereoZoom Optical system Leica S4E 12 Greenough using b
4. User Manual Apochromatic correction The Leica S8 APO is a completely apochromati cally corrected Greenough system The modern optical technology of the StereoZoom Leica S8 APO corrects chromatic aberration perfectly removes bothersome color seams and displays pin sharp images of even the finest enlarged detail Contrast brilliance image sharpness resolution color fidelity and image precision are unsurpassed The benefit of apochromatic correction is best seen in specimens that have a fine low contrast structure such as large animal cells cilia plants or metallic microelectronic structures The technical features of the individual models are found on page 52 The model series Leica S4E Leica S6E Leica S6 Leica S6T Leica 6 D Leica S8 APO Leica S Series User Manual On We Go If your new Leica stereomicroscope has already been assembled and commissioned by your Leica consultant click here to skip through the installation instructions and go directly to the Quick Start Guide on Page 26 Leica S Series If on the other hand you are assembling the stereomicroscope yourself continue with the Assembly chapter which begins on Page 16 User Manual Assembly Installing the basic equipment overview Leica S Series 1 Sub base for transmitted light with glass stage plate 2 Incident light base with stage plate 3 Focusing column with microscope carrier 4 Additional objective opt
5. when changing magnification The focus needs to be readjusted only if you want to view a specimen detail that is located higher or lower Use this advantage as often as possible it is not available on all stereomicroscopes Gi The diopters can be set between 5 and 5 Leica S Series The following adjustments have to be Li carried out only once by each user Using reticules leads to slightly deviated settings which are described in the user manuals on the reticules measurement User Manual Adjusting the diopter settings 1 With Leica S6 D and Leica 8 APO turn the rotary knob to the Vis setting 2 Turn the dioptric correction on both eyepi eces to the center position Continued on next page Dioptric correction with two adjustable eyepieces continued 3 Place a flat specimen under the objective 6 Set the highest magnification level 9 Turn the eyelens of the eyepiece as far as it will go in the direction without looking 4 Set the lowest magnification level 7 Optimize the focusing with the focusing into the eyepieces while doing so drive 10 Look through the eyepieces and close one eye 11 With the other eye monitor the specimen and turn the eyelens of the eyepiece slowly in the direction until this eye sees the specimen sharply 5 Observe the specimen through the eyepi eces and bring it into sharp focus with the 8 Set the lowest magnification level 12 Repeat steps 1
6. phy or in use combination with the Leica S8 APO we offer different high performance transmitted light stands and LED incident light illumination as in the Leica LED3000 series for example Please ask your Leica consultant about the options User Manual Camera design Leica S6 D and S8 APO The Leica S6 D and S8 APO are equipped with an integrated video phototube which allows the simple fast mounting of digi tal cameras for photos and video Please ask your Leica consultant about the options Detailed information about the availa ble camera systems accessories and the software packages from Leica can be found in the corresponding manuals Leica S Series Installing the camera 3 Insert the unit into the video photo output 1 Remove the protective dust cover from the of the stereomicroscope and screw it in video projection lens C mount adapter and the microscope camera Always close the video photo output with the protective dust cover if no 2 Screw the camera into the video projection camera is installed lens C mount User Manual Quick Start Guide An overview of an S series stereomicroscope 1 Magnification changer right drive knob with magnification scale 2 56 S8 models Stop for zoom limit 3 Focusing drive 4 Fixing screw for optics carrier in the microscope carrier 5 Adjustable tube Interpupillary distance adjustable from 55 to 75mm 6 Eyepieces 7 Threads for fastening the light
7. the graticule correctly Eyepieces You can use your Leica StereoZoom Li together with a fixed or adjustable eyepi ece For models in which a graticule is included in an eyepiece for measurement or photogra phy two eyepieces are necessary We recom mend that you also equip the high powered StereoZoom Leica S8 APO with two adjustable eyepieces Leica S Series Inserting the eyepieces 1 Push eyepieces as far as they will go into the tubes 2 Check that the eyepieces are seated firmly and precisely in place User Manual Risk of infection Direct contact with eyepieces is a poten Li tial transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Leica LED illumination The Leica KL300 LED cold light source with its fiber optic light guides is ideally suited for the Leica S4 E S6 E S6 and S6 T stereomicroscopes A number of matching adapters are available for connecting the Leica KL300 LED cold light source to various stereomicroscope stands and for stand alone operation For detailed information about installation and use please refer to the user manual for the Leica KL300 LED A Please note that the universal light guide on the Leica S8 APO can only be used with the light arm mounted on the side Leica S Series High output illuminators For higher demands such as photogra A
8. 0 and 11 with the other eye focusing drive Continued on next page Leica S Series User Manual Dioptric correction with two adjustable eyepieces continued Checking parfocality 1 Select the highest magnification level 2 Monitor the specimen if necessary refocus it slightly 3 Change from the highest to the lowest magnification The sharpness should be constant parfocal If this is not the case repeat this procedure Leica S Series User Manual Photography amp Video Photography amp Video For most stereomicroscope users digital docu mentation has become an invaluable part of their work Research results can be presented in an appealing form while measurements on the digital image provide clarity Adapters If camera control using the Leica Application Suite is not required conventional mirror reflex and rangefinder cameras from third party manufacturers can be used Leica Microsystems offers various adapters for these Leica S Series Leica DFC cameras If you require absolute control over the camera and need the capability for measurement evaluation and more in addition to photogra phy the digital Leica DFC cameras are exactly right for you Together with the Leica Applica tion Suite they provide virtually limitless free dom of use For additional information about Leica cameras refer to the camera s documen tation User Manual Leica Application Suite The Leica Application S
9. 10x 23 16x 15 25x 9 5 40x 6 10x 23 16x 15 25x 9 5 40x 6 Interpupillary distance 55 75 mm 55 75 mm 55 75 mm 55 75 mm User Manual 55 75 mm 55 75 mm Appendix Leica S Series User Manual Calculating the total magnification field of view diameter Parameter Example Mo Magnification of the additional objective Mo Additional objective 1 6x ME Magnification of eyepiece ME 20x 12 eyepiece Z Magnification changer position Z Zoom position 4 0 Nfov Field number of the eyepiece Field numbers are printed on the eyepieces 10x 23 16x 16 20x 12 10x 23B 16x14B 25x 9 5B 40x6B Magnification in the binocular tube Calculation example Field of view diameter in the specimen MoT vis Mo x ME xz ol 1 6 X 20 x 4 128 Leica S Series User Manual Troubleshooting The field of view is shadowed Adjusting the correct Distance P 52 Interpupillary The image goes out of focus Inserting the eyepieces correctly P 55 Perform diopter correction exactly accor ding to the instructions from P 31 The focusing drive gradually sinks on its own or is difficult to turn Regulate the ease of movement P 30 Leica S Series In the case of failures of electrically opera ted devices always first check Is the voltage selector set correctly Is the main power switch switched on Is the power cable connected correctly Are all connecting cable
10. 3B wide field eyepiece for eyeglass wearers it is approx 22 mm For those who do not use the eyepiece for eyeglass wearers it is 12mm Leica S Series Setting the eye distance 1 Bring the eyes slowly to the eyepieces Push the tubes together or apart with both hands until you see a single round circular image field without shadows with both eyes User Manual Focusing Focusing raises and lowers the stereomi Li croscope using the focusing drive The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective fil The focusing drive can be operated either left or right handed Leica S Series Focusing 1 Align the specimen under the objective 2 Set the lowest magnification level i At the lowest magnification the desired specimen detail can be easier to localize due to the large field of view User Manual 3 Look into the eyepieces and insert the desi red specimen detail in the center 4 Focus on the specimen with the drive knob Changing magnification zooming All stereomicroscopes of the S series Li allow a continuous magnification change The magnification changer can be operated with the left and the right hand The image scale is shown on the right drive knob The basis for the calculation of the total magnification and the field of view can be found on page 26 Leica S Series Changing magnification 1 Look into the eyep
11. 4 0 144 0 2 Aperture Working distance basic 110 mm 110 mm 110 mm 110 mm 110 mm 75mm config Object field diameter 36 5 mm 36 5 mm 36 5 mm 36 5 mm 36 5 mm 23 mm Adjustable zoom limits 2 2 2 2 2 Video photo outlet 100 visual or 100 100 visual or 100 video photo and 100 switchable video photo and 100 visual in the left eyepiece visual in the left eyepiece Image acquisition coax lighting Yes Yes Standard objectives lead free Achromats Achromats Achromats Achromats Achromats Apochromats 0 63x 1 6x 2 0x 0 32x 0 5x 0 63x 0 32x 0 5x 0 63x 0 32x 0 5x 0 63x 0 32x 0 5x 0 63x 0 32x 0 5x 0 63x Achromat 0 32x ErgoObjective 0 6x 0 75x 77 137 mm 0 7x 1 0x 48 98 mm 0 6x 0 75x 77 137 mm 0 7x 1 0x 48 98 mm 0 6x 0 75x 77 137 mm 0 7x 1 0x 48 98 mm 0 6x 0 75x 77 137 mm 0 7x 1 0x 48 98 mm 0 6x 0 75x 77 137 mm 0 7x 1 0x 48 98 mm Adjustable Objectives 0 3x 0 4x 200 350 mm 0 3x 0 4x 200 350 mm 0 3x 0 4x 200 350 mm 0 3x 0 4x 200 350 mm 0 3x 0 4x 200 350 mm Ergonomic eyepieces fixed and adjustable with cups 10x 23 16 16 20x 12 10x 23 16x 16 20x 12 10x 23 16x 16 20x 12 10x 23 16x 16 20x 12 10x 23 16x 16 20x 12 Ergonomic eyepieces for eyeglass wearers adjustable with eyecups 10x 23 16x 15 25x 9 5 40x 6 10x 23 16x 15 25x 9 5 40x 6 10x 23 16x 15 25x 9 5 40x 6 10x 23 16x 15 25x 9 5 40x 6
12. 6 8 625 45 45 8625 45 10 Switzerland Heerbrugg 41 717263434 71726 3444 Singapore 65 6779 7823 6773 0628 Spain Barcelona 34 934949530 93 494 95 32 USA Buffalo Grove lllinois 1 8002480123 847 405 0164 People s Republic of China Hong Kong 852 25646699 2564 4163 Shanghai 86 21 6387 6606 21 6387 6698 101DS10060EN Copyright by Leica Microsystems Schweiz AG CH 9435 Heerbrugg 2013 Subject to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH eca MICROSYSTEMS
13. 6 D with incident light and transmitted light illumination 49 Mounting a camera Leica S6 D and S8 APO 25 Leica S8 APO with incident light and transmitted light illumination 50 Leica S Series User Manual Overview of technical features 52 Specifications 53 Calculating the total magnification field of view diameter 55 Troubleshooting 56 Care Maintenance Contact Persons 57 Leica S Series User Manual Leica S series Congratulations Congratulations on obtaining your new Leica StereoZoom line stereomi croscope S series We are convinced it will exceed your expectations as this instrument embodies all the qualities you associate with the name Leica Microsystems excellent objectives high quality engineering and reliability Furthermore the modular design ensures that the Leica stereo microscope adapts perfectly to your needs no matter which accessories you require for your tasks Thanks to the parfocal system with simultaneously large working distan ces and object fields you can always view your microscopic specimens accurately from the complete image to the finest detail Leica S Series Though the reliability and robustness of Leica stereomicroscopes is world renowned like any high tech product the Leica S series requires a certain degree of care and attention Therefore we recommend that you read this manual It contains all the information you need regarding operation safety an
14. Living up to Life Seica MICROSYSTEMS User Manual Leica S series i General Instructions Safety concept Before using your stereomicroscope for the first time please read the Safety concept brochure included with your instrument It contains additional information about handling and care Bezpe nostn koncepce Sikkerhedskoncept Sicherheitskonzept Ohutuseeskiri Safety concept _ Concepto de seguridad Concept de s curit Concetto di sicurezza Dro bas noteikumi Saugos samprata Biztons gi el r sok Veiligheidsconcept Sikkerhetskonsept Koncepcja bezpiecze stwa Conceito de seguran a Bezpe nostn pokyny Varnostni koncept Turvallisuus S kerhetskoncept RES geri Leica S Series Use in clean rooms The Leica S series can be used in clean rooms without any problems About cleaning Do not use any unsuitable cleaning agents chemicals or techniques for cleaning Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abra ded particles In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms User Manual Servicing Repairs may only be carried out by Leica Microsystems trained service technicians Only original Leica Microsystems spare parts may be used
15. arm both sides and rear 8 Illumination Leica LED3000 SLI 9 Illumination Leica LED3000 RL 10 Leica microscope camera 11 C mount adapter and video photo tube Leica S Series User Manual Tips for working ergonomically Align your stereomicroscope optimally You must configure the settings descri bed here precisely in order to be able to take full advantage of its outstanding optical and ergonomic advantages Align your workstation optimally Consider the height of the bench and chair Use the whole seat surface and the back rest Ensure that your lower arms are supported When carrying out other tasks perform exercises to relax and relieve muscle tension Leica S Series Ergonomic objectives The ErgoObjective for the Leica S4 E and all S6 models provides for fatigue free work The ErgoObjective 0 6 0 75x with an adjustable working distance of 77 137 mm and the Ergo Objective 0 7 1 0x with an adjustable working distance of 48 98 mm allow the fine adjust ment of the working distance the magnifica tion and the viewing level without time consu ming changing of lenses The Leica S6 with a 60 viewing angle offers optimum viewing height on the inclined stereomicroscope User Manual Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer Simply push them into the tube and they are ready to use What does parfocal mean
16. bjective F Leica S Series User Manual Available Graticules The optional graticules enable measure ment and in addition provide valuable information when comparing and capturing still images of the specimens Insert the grati cule before you set the eyepiece in place Leica S Series Available Graticules The following graticules and objective micro meters for calibrating may be ordered Graticule 10 mm 0 1 mm Graticule 5 mm 0 1 mm Graticule 5 mm 0 05 mm Graticule 100 Div 0 002 Graticule 100 Div 0 001 Graticule 150 Div 0 0005 Crosshairs Stage micrometer 50 mm 0 1 0 01 mm graduation Stage micrometer 1 0 001 graduation User Manual Inserting graticules A Graticules can be inserted in the adjus table eyepieces and the in the eyepieces for eyeglass wearers A The procedure for taking measurements is described in the Measuring user manual Leica S Series Inserting graticule s 1 Use the stereomicroscope to determine the side on which the scale is inscribed The scale must not appear reversed Remove the insert from the bottom of the eyepiece and place it on the bench with the knurled side down User Manual Hold the graticule by the edges to avoid leaving fingerprints and push it into the holder from the side Replace the insert in the eyepiece and press it firmly into place Insert the eyepiece in the tube and turn the eyepiece in the tube to align
17. cally intended for that purpose The devices and accessories described in this User Manual have been tested with regard to potential hazards The responsible Leica affiliate must be consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Leica S Series Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use Refer to Safety Concept booklet Electrical components must be placed at least 10 cm away from the wall and from flammable substances Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort measurements and micro graphic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus User Manual Responsibilities of person in charge of instrument Refer to Safety Concept booklet Ensure that The S series stereomicroscopes and acces sories are operated maintained and repaired by authorized and trained person nel only All operators have read understood and observe this User Manual and particularly the safety instructions Safety Instructions continued Repairs service work Refer to Safety Concept booklet Only original Leica Microsystems spare parts may be used Before opening the instruments s
18. close together so the stereomicro scopes can be of very slender design espe cially towards the base of the instrument The advantage The advantages of this design are that it has a small space requirement for use on bonders and in machine applications unobst ructed access to specimens plenty of space for tools and a completely clear view of the object field The Greenough system enables cost effective correction of aberrations such as chromasia image field curvature and distortion with mini mal effort In the new Leica StereoZoom line the optimum corrected center of the objec tive is used for the image This provides supe rior optical performance with large level and undistorted fields of view and chromatically optimized high contrast images Leica S Series ESD protection The Leica S4 E S6 E S6 S6 D and S8 APO including its cold light source and stand is made from highly conductive material with surface resistivity of 2 10 Ohm square with a discharge time of lt 2 seconds 1000 V to 100 V The Leica S6 T terminator version for highly complicated working ranges and the T incident light stand consist of a dissipative material with a surface resistivity of 10 10 Ohm square and a discharge time of lt 0 1 seconds from 1000 V to zero Photography The StereoZoom models Leica S6 D and S8 APO are equipped with an integrated video phototube which allows the simple fast moun ting of digital cameras
19. d maintenance Simply observing a few guidelines will ensure that even after years of intensive use your stereomicroscope will continue to work as smoothly and reliably as on the very first day We wish you the best of success in your work after all you are now equip ped with the best tool User Manual The Modular Design Everything is possible The Leica S series provides a high degree of flexibility in choosing equipment thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades The optics carriers eyepieces stands and more can be combined in any way you choose allowing you to create the stereomicroscope that best suits your needs Leica S Series Despite this you will notice that the controls and individual components do not differ signi ficantly Whichever configuration you choose you will quickly feel right at home with your new stereomicroscope User Manual Have a special request Let us know Leica Microsystems enjoys an exceptional repu tation when it comes to devising customer specific solutions If you have a special request that cannot be met with standard parts contact your Leica consultant He or she has a solution for every application What your stereomicroscope has to offer you The optical system of the Leica StereoZoom line consists of two beam paths converging at 12 The objective pairs of each optical path are positioned
20. d parfocality 1 adjustable amp 1 fixed eyepiece continued 3 Place a flat specimen under the objective 6 Set the highest magnification level 9 Turn the eyelens of the eyepiece as far as it will go in the direction without looking 4 Set the lowest magnification level 7 Optimize the focusing with the focusing into the eyepieces while doing so drive 10 Close your eye on the fixed eyepiece and look with the other eye into the into the adjustable eyepiece 11 Rotate the eyelens of the eyepiece slowly in the direction until the eye can see the specimen sharply 5 Observe the specimen through the eyepi eces and bring it into sharp focus with the 8 Set the lowest magnification level Continued on next page focusing drive Leica S Series User Manual Diopter settings and parfocality 1 adjustable amp 1 fixed eyepiece continued Checking parfocality 1 Select the highest magnification level 2 Monitor the specimen if necessary refocus it slightly 3 Change from the highest to the lowest magnification The sharpness should be constant parfocal If this is not the case repeat this procedure Leica S Series User Manual Dioptric correction with two adjustable eyepieces If you set the diopters on the adjustable Li eyepiece exactly as described the image will remain equally sharp and constant parfo cal from the lowest to the highest magnifica tion This means you do not have to refocus
21. est Leica S6 12 Greenough using best Leica S6E 12 Greenough using best Leica S6T 12 Greenough using best Leica S6 D 12 Greenough using best Leica S8 APO 12 Greenough using best lead free corrected central partof corrected central partof corrected central partof corrected central part ofthe corrected central part of corrected central part of the objective the objective the objective objective the objective the objective Zoom 4 8 1 6 3 1 6 3 1 6 3 1 6 3 1 8 1 apochromatic Viewing angle 38 60 38 38 38 38 ESD protection antistatic antistatic antistatic Terminator dissipating antistatic antistatic Specific surface 2x10 Q square 2x10 0 square 2x10 0 square 10 10 0 square 2x10 0 square dischar 2x10 Q square 0 75x 1 6x 2 0x 0 75x 1 6x 2 0x 0 75x 1 6x 2 0x 0 75x 1 6x 2 0x 0 75x 1 6x 2 0x resistivity discharge time discharge time discharge time discharge time ge time discharge time lt 2 seconds from lt 2 seconds from lt 2 seconds from lt 0 1 seconds from 1000 V lt 2 seconds from lt 2 seconds 1000 V to 100V 1000 V to 100V 1000 V to 100V to zero 1000 V to 100V from 1000 V to 100V Magnification basic confi 6 3x 30x 6 3x 40x 6 3x 40x 6 3x 40x 6 3x 40x 10x 80x guration Maximum resolution 372 Ip mm 432 Ip mm 432 Ip mm 432 Ip mm 432 Ip mm 600 Ip mm Maximum num About the 0 124 0 144 0 144 0 14
22. ieces 2 Focus on the object see page 16 3 Rotate the magnification changer until the desired magnification is configured User Manual Limiting zoom range With the S6 models and the S8 APO the zoom Defining the lower limit 3 Set the stop on the left drive knob forward range can be limited at the top and bottom In 1 Loosen the hollow screw on the left drive until it touches the built in zoom stop the same manner a fixed magnification level knob with the Allen key provided can be set The following example shows the limit to the range between 1 to 3 2 2 Turn the right drive knob to position 1 4 Carefully tighten the Allen screw Continued on next page Leica S Series User Manual Limiting zoom range continued Defining the upper limit stop 3 Set the stop backward on the right drive 1 Loosen the hollow screw on the right drive knob until it touches the built in zoom knob with the Allen key provided stop 4 Carefully tighten the allen screw Leica S Series User Manual Regulating the resistance of the focusing drive Adjusting the resistance Is the focusing drive too loose or too tight Does the equipment tend to slide downwards The resistance can be adjusted individually depen ding on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focu
23. ional 5 StereoZoom optics carrier 6 Eyepieces fixed and or adjustable User Manual Focusing column A Never unscrew the 3 screws on the right Focusing column on the incident light base 3 Insert the stage plate back into place side of the focusing column 1 Removethe stage plate 2 Route the 3 hollow screws from below through the base plate and screw these securely into the focusing column Leica S Series User Manual Sub base for transmitted light and cold light source 1 Remove the glass stage plate 4 Press the locking mechanism backward 6 Insert the universal light guide in the The incident light base and the sub base opening at the rear 2 Pull the locking mechanism forward for transmitted light are now connected 3 Set the incident light stand to the sub base 5 Insert the glass stage plate for transmitted light and engage it with the A Additional information can be found in connection screw the User Manual for cold light source Leica KL300 LED Leica S Series User Manual Optics carrier and additional objective Optics carrier Additional objective optional Protective objective glass optional 1 Insert the optics carrier carefully in the 1 Screw the desired objective counterclock 1 Screwtheobjective protective glass directly microscope carrier and fasten it in the desi wise into the optics carrier onto the StereoZoom or on the additional red position with the clamping screw o
24. oped our five corporate values pioneering high end quality team spirit dedication to science and continuous improvement We call making these values reality Living up to Life INDUSTRY DIVISION The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result by providing the best and most innovative imaging systems for their needs to see measure and analyze microstructures Its solutions are used in routine and research industrial applications in materials science and quality control in forensic science investigations and educational applications www leica microsystems com Leica Microsystems an international company with an experienced worldwide customer service network Internationally active Phone Fax Australia North Ryde 61 288703500 2 9878 1055 Belgium Diegem 32 27909850 2790 98 68 Denmark Ballerup 45 44540101 44540111 Germany Wetzlar 49 6441294000 64 41 29 4155 England Milton Keynes 44 8002982344 1908 246312 France Nanterre Cedex 33 811000664 15605 23 23 Italy Milan 39 02 574 861 02 574 03392 Japan Tokyo 81 3 5421 2800 3 5421 2896 Canada Concord Ontario 1 8002480123 847 405 0164 Korea Seoul 82 2514 65 43 2514 65 48 The Netherlands Rijswijk 31 70 4132 100 70 4132 109 Austria Vienna 43 148680500 1 486 80 50 30 Portugal Lisbon 351 21 388 9112 21 385 4668 Sweden Kista 4
25. rtion of the user to a minimum A Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups User Manual Table of Contents General Instructions 2 Important Safety Notes 3 A stereomicroscope of the S series in overview 27 Symbols Used 4 Tips for working ergonomically 28 Safety Instructions 5 Using the Eyepieces 29 Table of contents 8 The correct interpupillary distance 30 Focusing 31 Switching magnification zooming 32 Congratulations 11 Limiting zoom range 33 The Modular Design Everything is Relative 12 Regulating the resistance of the focusing drive 35 What your stereomicroscope has to offer you 13 Changing the position of the optics carrier 36 The model series 14 Diopter settings and parfocality 1 adjustable amp 1 fixed eyepiece 37 On We Go 15 Dioptric correction with two adjustable eyepieces 40 Installing the basic equipment overview 17 Photography amp Video 44 Focusing column 18 Photography with the Leica S6 D and S8 APO 45 Sub base for transmitted light and cold light source 19 Optics carrier and additional objective 20 Available graticules 21 Leica S6 E S4 E S6 T with incident light and transmitted light Inserting graticules 22 illumination 47 Eyepieces 23 Leica S6 48 Leica LED illuminators 24 Leica S
26. s attached correctly Are the fuses intact User Manual Photos are blurry Focus accurately P 23 Bring the reticule into sharp focus and perform diopter correction exactly accor ding to the instructions P 37 Insert the eyepieces correctly up to the stop P 35 Check that the graticules are securely in place in the eyepiece P 31 The image from the camera stays black Switch the beam splitter on the photo tube to the Doc setting P 37 Care Maintenance Contact Persons We hope you enjoy using your stereomicro scope Leica devices are renowned for their robustness and long service life Observing the following care and cleaning tips will ensure that even after years and decades your Leica stereo microscope will continue to work as well as it did on the very first day Warranty benefits The guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Leica S Series Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Schweiz AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com User Manual Care Protect your stereomicroscope from mois ture fumes and acids and from alkaline caustic and corrosive materials and keep chemicals away from the instruments Plugs optical sy
27. sing Leica S Series User Manual Changing the position of the optics carrier The optics carrier can be turned side Changing position ways in the microscope carrier if the user 1 Unscrew the clamping screw wants to work from the side 2 Turn the optics carrier laterally to the desi red position 3 Carefully tighten the clamping screw Leica S Series User Manual Diopter settings and parfocality 1 adjustable amp 1 fixed eyepiece If you set the diopters on the adjustable Li eyepiece exactly as described the image will remain equally sharp and constant parfo cal from the lowest to the highest magnifica tion This means you do not have to refocus when changing magnification The focus needs to be readjusted only if you want to view a specimen detail that is located higher or lower Use this advantage as often as possible it is not available on all stereomicroscopes Gi The diopters can be set between 5 and 5 Leica S Series The following adjustments have to be Li carried out only once by each user Using reticules leads to slightly deviated settings which are described in the user manuals on the reticules measurement User Manual Adjusting the diopter settings 1 With Leica S6 D and Leica S8 APO turn the rotary knob to the Vis setting 2 Turn the dioptric correction on the adjus table eyepiece to the center position Continued on next page Diopter settings an
28. stems and mechanical parts must not be disassembled or repla ced unless doing so is specifically permit ted and described in this manual Protect your stereomicroscope from oil and grease Do not grease guide surfaces or mechanical parts Care Maintenance Contact Persons continued Protection from dirt Dust and dirt will affect the quality of your results Puta dust cover over the stereomicroscope when it will not be used for a long time Use dust caps to protect tube openings tubes without eyepieces and eyepieces Keep accessories in a dust free place when not in use Leica S Series Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsu itable cleaning agents and techniques can damage polymers User Manual Permitted measures Clean the stereomicroscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety regulations Remove dust with a pneumatic rubber bulb or with a soft brush Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol The productive cooperative effort with the user for the user has always been the basis for the innovative strength of Leica Microsystems On this we have devel
29. tained in these user manuals User Manual Symbols Used Warning of a danger This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged inst ruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged inst ruments Leica S Series Danger due to hot surface A This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Explanatory notes This symbol within the text stands for addi tional information and explanations Figures 1 Numbers in parentheses within the descriptions relate to the figures and the items within those figures User Manual Safety Instructions Description The individual modules fulfill the highest requi rements for observation and documentation of Leica stereomicroscopes of the S series Intended use Refer to Safety Concept booklet Non intended use Refer to Safety Concept booklet Never use S series microscopes or their compo nents for surgical procedures e g on the eye unless they are specifi
30. uite or LAS for short is as it were the digital extension of your stereomicroscope With it you can not only take images but also control the illumination the camera and more For additional information refer to the LAS online help Photography with the Leica S6 D and S8 APO A The observation beam path and the photobeam path can be switched For this the light splitting is influenced as follows Position Vis 100 light in both eyepie ces but no light in the video photo beam path Position Doc 100 light in the right eyepiece but no light in the left eyepiece 100 of the light crosses the video photo beam path Leica S Series The focusing and the framing are done with the left eyepiece video photo beam path Flat specimens are sometimes partially blurry on the left and right edge of the image This blurring is based on laws of optics and does not mean there is a malfunction of the camera or the microscope User Manual Capturing images and videos 1 Ifthe image detail and image sharpness are set to your satisfaction switch on the Doc setting and capture your image Dimensional drawings in mm Leica S6 E S4 E S6 T with incident light and transmitted light illumination papa q aa o 223 Leica S Series User Manual Leica S6 915 8 25 atte
31. witch off the power and unplug the power cable Touching the live circuit can cause injury Leica S Series Transport Use the original packaging for shipping or transporting the individual modules of the Leica S stereomicroscopes and the acces sory components In order to prevent damage from vibra tions all moving parts that according to the user manual can be assembled and disassembled by the customer should be disassembled and packed separately User Manual Integration in third party products Refer to Safety Concept booklet Disposal Refer to Safety Concept booklet Legal regulations Refer to Safety Concept booklet EC Declaration of Conformity Refer to Safety Concept booklet Safety Instructions continued Health risks Workplaces with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken Optimal arrangement of workplace work assignments and work flow changing tasks frequently Thorough training of the personnel giving consideration to ergonomic and organizati onal aspects Leica S Series The ergonomic optical design and const ruction of the Leica S stereomicroscopes are intended to reduce the exe
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