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User Manual - Meadows Instrumentation

1. 135 Repairing the Autosampler Sampling Unit 6 Turn the tray base locking screw 4 turn 7 Slide the tray base back to disengage the tray anti clockwise base from the sampling unit 136 Repairing the Autosampler Sampling Unit Installing the Sampling Unit NOTE The replacement sampling unit is supplied without injection valve and metering head assembly If you are exchanging the complete sampling unit remove the injection valve and metering head from the defective sampling unit Install the valve and metering head in the new sampling unit See Injection Valve Assembly on page 140 and Gripper Arm on page 123 1 Mount the tray base onto the sampling unit Ensure the tray base is positioned flush against the sampling unit 2 Tighten the locking screw 1 turn clockwise Correct positioning of the tray base 137 Repairing the Autosampler Sampling Unit 3 While holding the sampling unit and tray base slide the leak sensor into the holder in the leak plane 4 Place the sampling unit and tray ba
2. Figure 42 Board identification E E Processor BCD register N 12 BCD lt ___ _ gt L gt interface Pa connector Line driver o 0m ce External External contact gt contacts A 4x connector LAN Board The HP JetDirect cards are network interface cards used in HP printers NOTE One board is required per Agilent 1100 stack It is recommended to add the LAN board to the detector with highest data rate NOTE The LAN board can only be used together with a main board version G13XX 66520 DAD MWD VWD Pump ALS or G13XX 66500 FLD RID and above e a Agilent ChemStation software revision A 06 01 or above 229 Table 48 NOTE Theory of Operation Optional Interface Boards The following cards can be used with the Agilent 1100 modules LAN Boards Agilent Order Number Supported networks J4106A Ethernet 802 3 RJ 45 10Base T J4105A Token Ring 802 5 DB9 RJ 45 10Base T J4100A Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 Minimum firmware of the JetDirect cards is A 05 05 Recommended Cables For point to point connection not using a network hub use a twisted pair cross over LAN cable P N 5183 4649 10 feet long For standard network connections using a hub use category 5 UTP cables P N G1530 61480 8 m long 230 Theory of Operation Interfaces Interfaces
3. Remote cable CAN Bus cable to control module ooo0000 0000000 CAN Bus cable AC power oo e 000000 ooo0000 o Analog signal to recorder GPIB or LAN to LC ChemStation 23 Installing the Autosampler Optimizing the Stack Configuration Figure 3 Recommended Stack Configuration for a Thermostatted ALS Front View Detector EEG k o Control Module SK ee O jazm a t a a i TT i ee Column VX a Compartment mE y Se L Co 5 Solvent Cabinet Autosampler or 1 Micro ALS or Preparative ALS J S e Ss a Degasser t Cc ALS Thermostat Pump al oy iz ST ar ES F 24 Installing the Autosampler Optimizing the Stack Configuration Figure 4 Recommended Stack Configuration for a Thermostatted ALS
4. Connector Pin Pin Active 01046 60201 Universal Agilent 1100 Signal Name TTL 1 White Digital o ground ie i ol l 2 Brown Prepare run Low oo Dp KEY 3 Gray Start Low o on E jpo 4 Blue Shut down Low EE ac 5 Pink Not o0 connected oo Jof 6 Yellow Power on High 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 194 Parts and Materials BCD Cables BCD Cables One end of these cables provides a 15 pin BCD connector to be connected to the Agilent 1100 Series modules The other end depends on the instrument to be connected to Agilent 1100 to 3392 3 Integrators Connector Pin Pin 18584 60510 3392 3 Agilent 1100 Signal Name BCD Digit 10 1 BCD 5 20 11 2 BCD 7 80 3 3 BCD 6 40 9 4 BCD 4 10 7 5 BCD 0 1 5 6 BCD 3 8 12 7 BCD 2 4 4 8 BCD 1 2 6 Key 1 9 Digital ground 2 15 5V Low 195 Parts and Materials BCD Cables Agilent 1100 to 3396 Integrators Connector Pin Pin 03396 60560 3392 3 Agilent 1100 Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3e 45 3 3 BCD 6 40 F 4 4 BCD 4 10 o 5 5 BCD 0 1 e 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low Agilent 1100 to General Purpose Connector Pin 18594 60520 Wire Color Agilent 1100 Signal Name BCD Digit Green 1 BCD 5 20 Violet 2 BCD 7 80 Blue 3 BCD 6 40 ie a Yellow 4 BCD 4 10 D
5. 149 Repairing the Autosampler ASM Board 7 Install the transport assembly page 132 8 Install the top cover and foam page 128 9 Turn on the autosampler 10 11 NOTE Enter the 10 character autosampler serial number The serial number can be entered using either the control module or the ChemStation see Entering the Serial Number using the Control Module on page 150 or see Entering the Serial Number using the ChemStation on page 151 Check the firmware revision of the autosampler If the firmware revision is older than the current firmware revision of the autosampler update the firmware see Replacing the Autosampler Firmware on page 151 Entering the Serial Number using the Control Module Connect the control module to the autosampler Turn on the autosampler In the control module press System F5 then Records F4 Using the up down arrows make sure that the autosampler is highlighted Press FW Update F5 Now press the m key This will display a box which says Update Enter Serial Press Enter This will display the box labeled Serial Letters and numbers are created using the up and down arrows Into the box labeled Serial enter the 10 character serial number for the autosampler When the 10 character serial number is entered press Enter to highlight the complete serial number Then press Done F6 For firmware revisions below A02 00 it is very important ne
6. lt 6 os lia ll 21 Install the metering head assembly see Gripper Arm on page 123 On completion of this procedure 1 Install the top cover page 128 1 Verify the transport assembly alignment page 99 145 Repairing the Autosampler Fan KEES Fan Frequency When defective Tools required None Parts required Fan 3160 1017 CAUTION The ASM board is sensitive to electrostatic discharge Always use the ESD strap see Using the ESD Strap on page 105 when handling electronic boards E Before beginning this procedure 1Disconnect the fan connector from the ASM board L Remove the top cover and foam page 128 Li Remove the transport assembly page 132 Y fv UAAR RARA ARAR AHA y 2 Lift the fan and carefully pull the fan out of 3 Install the new fan Ensure the cable is the foam installed as shown 146 Repairing the Autosampler Fan 4 Connect the fan connector to the ASM board a On completion of this procedure 4 Install the transport assembly page 132 1 Install the top cover and foam page 128 Ll Verify the transport assembly alignment page 99 147 Repairing the Autosampler ASM Board ASM Board Frequency Tools required Parts required When defective 5 mm wrench for remote connector screws 7 m
7. 5 Lower the cover into place Ensure the clips 6 Slide the power lock to the right and install close firmly the power cable Q gt a pae a 3 F n r a poe ERE Oa Sa ke Tl uaa a Sc DET GERMANY 7 fo S fke ITE i rA oe N 131 Repairing the Autosampler Transport Assembly Transport Assembly Frequency Sticking or jammed transport assembly Defective flex board or sensors Tools required If interface board installed Flat head screwdriver Parts required Transport assembly G1313 60009 for G1373A Transport assembly G1329 60009 for G1329A 1389A G2260A 1 Remove the top cover top plate and foam see Top Cover and Foam on page 128 2 Lift out the transport assembly This may require a flat head screwdriver to separate the transport assembly from the sampling unit Figure 18 Removing the Transport Assembly AEE WOO IOQE MQSQ Aint N N 132 Repairing the Autosampler Transport Assembly Installing the Transport Assembly 1 Slide the transport assembly into the autosampler 2 Ensure the slot on the top of the transport assembly engages with the stud on the sampling unit N Ra WWWQQ QS RSS SOOO Ss NN NY Transport assembly Sampling unit On completion of this procedure 1 Install the top co
8. Copyright Agilent Technologies 2001 All rights reserved Reproduction adaption or translation without prior written permission is prohibited except as allowed under the copyright laws Part No G1313 90004 Edition 08 01 Printed in Germany Warranty The information contained in this document is subject to change without notice Agilent Technologies makes no warranty of any kind with regard to this material including but not limited to the implied warranties or merchantability and fitness for a particular purpose Agilent Technologies shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material WARNING For details of safety see Safety Information on page 279 Warning Symbols Used In This Book The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Agilent 1100 Series Standard Micro and Preparative Autosamplers Reference Manual In This Book This manual contains technical reference information about the Standard Micro and Preparative Autosamplers and their respective Thermostatted version The manual describes the following e installing the autosampler
9. TT KE 12 Feed the motor cable through to the SUD 13 Install the motor with the four fixing screws board Connect the cable to labelled Ensure the belt is seated correctly over the Metering M gear and motor shaft On completion of this procedure LI Install the sampling unit page 134 1 Install the transport assembly page 132 1 Install the top cover page 128 1 Verify the transport assembly alignment page 99 143 Repairing the Autosampler Needle Drive Motor and Belt Needle Drive Motor and Belt Frequency When defective Pozidrive No 1 screwdriver Needle drive motor 5062 8590 Belt 1500 0697 Tools required Parts required ee Before beginning this procedure L Remove the top cover page 128 14 Lift away the drive belt 15 Disconnect the motor connector from the SUD 16Remove the metering head assembly see Gripper Arm on page 123 board labelled Needle M 5y F 144 Repairing the Autosampler Needle Drive Motor and Belt 17 Remove the four fixing screws and remove the motor 18 Feed the motor cable through to the SUD board Connect the cable to the connector labelled Needle M 7 19 Secure the motor in place with the four fixing screws 20 Install the belt 7 OY
10. On completion of this procedure Li Install the sampling unit page 134 LI Install the transport assembly page 132 1 Install the top cover page 128 1 Verify the transport assembly alignment page 99 153 Repairing the Autosampler Power Supply Power Supply Frequency When defective Tools required Pozidrive No 1 screwdriver Parts required Power supply 0950 2528 CAUTION The ASM board is sensitive to electrostatic discharge Always use the ESD strap see Using the ESD Strap on page 105 when handling electronic boards ey Before beginning this procedure 1 Remove the bottom foam Li Remove the top cover page 128 Li Remove the transport assembly page 132 Li Remove the sampling unit page 134 1 Remove the ASM board page 148 m Remove the fan page 146 2Remove the power switch light pipe from the coupler MADE See asesors MMII 7 154 5 Remove the coupler from the of the power supply 7 Install the power supply in the module 9 Install the bottom foam Ensure the cables are positioned as shown 155 Repairing the Autosampler Power Supply 4 Lift the power supply out of the module TINA JUL JU 6 Insert the coupler onto the switch of the new power supply 8 Connect the light
11. e Agreen status indicator indicates the instrument is performing an analysis run mode e A yellow indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed for example front cover not installed or while a self test procedure is running e An error condition is indicated when the status indicator is red An error condition indicates the instrument has detected an internal problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis 52 Troubleshooting and Test Functions Error Messages Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument log book This section describes the meaning of autosampler error messages and provides information on probable causes and suggested actions how to recover from error conditions 53 Troubleshooting and Test Functions Timeout Timeout The timeout threshold was exceeded Probable Causes e
12. The Agilent 1100 Series modules provide the following interfaces Table 49 Agilent 1100 Series Interfaces DA Detector Thermostatted MW Detector VW Detector Column Vacuum Interface Type Pumps Autosampler FL Detector RI Detector Compartment Degasser CAN Yes Yes Yes Yes Yes No GPIB Yes Yes Yes Yes Yes No RS 232C Yes Yes Yes Yes Yes No Remote Yes Yes Yes Yes Yes Yes Analog Yes No 2x 1x No Yes Interface board Yes Yes Yes Yes No No The vacuum degasser will have a special connector for specific use For details see description of main board WARNING e CAN connectors as interface to other Agilent 1100 Series modules e GPIB connector as interface to the Agilent ChemStation e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog Output connector s for signal output and e Interface slot for specific interfacing external contacts BCD LAN and so on For identification and location of the connectors Figure 6 on page 27 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations see External Tray G1313 60004 on page 184 231 Table 50 Theory of Operation Interfaces Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board GPIB Interface The GPIB conn
13. battery 221 safety information 282 BCD board 228 BCD LAN board 228 bench space 15 boards interface board BCD LAN 228 bypass 205 bypassing the autosampler 35 Cc cable connecting APG remote 23 25 connecting CAN 23 25 connecting GPIB 23 25 connecting LAN 23 25 connecting the ChemStation 23 25 connecting the power 23 25 cables 185 CAN interface 232 capillaries 31 change metering seal 83 change needle 81 choice of vials and caps 46 cleaning the autosampler 104 common electronics 220 condensation 16 configuration switch 236 control module Analysis screens 247 Config screens 259 Control screens 258 Error Log screen 260 Firmware Update screens 262 Info Log and EMF screen 261 Introduction to ALS screens 246 Maintenance screens 263 Method screens 257 Records screen 260 Run Times screen 254 Settings screens 250 Signal Plot screens 255 Status screens 255 System screen 258 Tests and Diagnostic screens 264 Thermostat screen 254 Timetable screens 252 D damaged packaging 17 delay 38 delay volume 35 38 delivery checklist 17 DRAW 46 draw speed 46 E EJECT 46 eject speed 46 electronics 218 ASIC 220 ASM board 220 battery 221 electronic fuses 221 fan drive 221 firmware 226 sample transport control 221 sampling unit control 223 sensors 219 electrostatic discharge 105 environment 14 16 error messages 50 53 arm movement failed 66
14. compensation sensor open 61 compensation sensor short 62 fan failed 63 ignition without cover 64 initialization failed 72 initialization with vial 75 invalid vial position 79 leak 58 leak sensor open 59 leak sensor short 60 metering home failed 73 missing vial 71 missing wash vial 78 motor failed 74 needle down failed 70 needle up failed 69 remote timeout 56 restart without cover 65 safety flap missing 76 shutdown 55 synchroniztion lost 57 time out 54 valve to bypass failed 67 valve to mainpass failed 68 vial in gripper 77 ESD strap 105 F failure 50 firmware 226 main system 226 resident system 226 updates 227 flow connections 31 fuses 14 BCD board 228 power supply 243 G GPIB default addresses 232 interface 232 gripper 210 alignment 50 external vials 50 287 Index gripper alignment 87 gripper fingers 210 gripper position verification 89 H half trays 32 half tray combinations 32 hall sensor 219 injection sequence 205 injection valve 202 207 209 injection volume precision 46 injection volumes 46 injection volumes less than 2 ul 46 installing the autosampler 26 flow connections 31 interface cables 26 power cable 26 safety 26 sample trays 32 installing the sample tray 32 installing the thermostatted autosampler interface cables 28 power cable 28 power cable and interface cable 29 preparation 28 safety 26
15. e autosampler started with the top cover and foam removed LI Replace the top cover and foam 65 Probable Causes Suggested Actions Troubleshooting and Test Functions Arm Movement Failed Arm Movement Failed The transport assembly was unable to complete a movement in one of the axes The processor defines a certain time window for the successful completion of a movement in any particular axis The movement and position of the transport assembly is monitored by the encoders on the stepper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated See figure Transport Assembly on page 210 for axes identification Arm Movement 0 Failed X axis Arm Movement 1 Failed Z axis Arm Movement 2 Failed Theta gripper rotation Arm Movement 3 Failed Gripper gripper fingers open close e Mechanical obstruction e High friction in transport assembly e Defective motor assembly e Defective transport assembly flex board e Defective ASM board Ensure unobstructed movement of the transport assembly Exchange the transport assembly Exchange the ASM board Ooo 66 Probable Causes Suggested Actions Troubleshooting and Test Functions Valve to Bypass Failed Valve to Bypass Failed The injection valve failed to switch to the bypass position The switching of the injection valve is monitored by two microswitches o
16. e troubleshooting and test functions e repairing the autosamplers e parts and materials e introduction to the autosamplers e screens of the local control module e specifications e safety and warranty TT G1313 90004
17. DSP XAxis lq Z Axis i theta 1 Quadrature 4 moa decoder Encoder feedback data heta 225 Theory of Operation Firmware Description Firmware Description The firmware of the instrument consists of two independent sections e anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 series modules Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C e memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization via APG remote e error handling e diagnostic functions and so on or module specific functions like e internal events such as metering device gripper and needle movements 226 NOTE Figure 41 Theory of Operation Firmware Description Firmware Updates Firmware updates can be done using your user interface e handheld control module with files from a PC card or e Agilent ChemStation with files from floppy disk The file
18. RSD of peak areas from 2000 5000 ul Typically lt 3 RSD of peak areas from 1 5 ul 1 ul from 5 ul sample in 100 ul microvial or 1 ul from 10 ul sample in 300 ul microvial 0 2 50 cp 100 x 2 ml vials in 1 tray 15 x 6 ml vials in tray Agilent vials only Typically 50 s depending on draw speed and injection volume 273 Specifications Performance Specifications 274 Legal Safety and Warranty Information Warranty Statement Warranty Statement All Chemical Analysis Products Agilent Technologies warrants its chemical analysis products against defects in materials and workmanship For details of the warranty period in your country call Agilent During the warranty period Agilent will at its option repair or replace products which prove to be defective Products that are installed by Agilent are warranted from the installation date all others from the ship date If buyer schedules or delays installation more than 30 days after delivery then warranty period starts on 31 day from date of shipment 60 and 61 days respectively for products shipped internationally Agilent warrants that its software and firmware designed by Agilent for use with a CPU will execute its programming instructions when properly installed on that CPU Agilent does not warrant that the operation of the CPU or software or firmware will be uninterrupted or error free Limitation of Warranty Onsite warranty servic
19. The fan provides a PWM signal which is proportional to the revolution This fan status signal is used for diagnostics Electronic Fuses The circuits that are connected to 36 V are fused on the board electronically Onboard Battery An onboard lithium battery buffers the electronic memories when the module is turned off For safety information on lithium batteries see Lithium Batteries Information on page 282 Autosampler Specific Electronics The autosampler specific functions provided by the electronics are e closed loop control of four axis vial handling servos e electric valve control e Needle unit control e metering device control Transport Unit Control The transport drive electronics use current controlled pulse width modulation PWM to drive the X Z O and gripper motors in closed loop servo control mode Dedicated electronics in the SGS L6506 provide the current control loop Commutation is done in FPGA logic SGS L6201 SMT output drivers are used for all four stepper motors Motor encoder signals are connected to the ASIC where the encoder quadrature decoded clock and the up down signal are used in the FPGA to provide instantaneous stepper motor commutation with respect to the motor rotor position Wiring between the autosampler main board ASM and the motors and encoders uses a flat band cable 64 pin and a flex board on which 10 221 Theory of Operation Autosampler Main Board ASM reflection light
20. Choose System from the pull down menu This screen shows the last activities in the system Time HOT Abort Ready Module Message Id Date Time Autosampler Door closed NF 07 02 14 53 10 Autosampler Door opened N 07 02 14 53 04 Autosampler Module method deleted INFD 07 02 14 51 49 Autosampler Module method saved N 07 02 14 51 22 Controller No analysis SIRE 07 02 14 49 01 Use the F1 key Control to select the Autosampler Here you receive information about the not ready conditions if needed F2 key Reset does a re initialization of the ALS Use the F8 key On Off to turn on the thermostat The F1 key Release Vial releases a vial held in the gripper the F2 key Home Gripper returns the gripper to the home position and the F3 key Park Gripper parks the gripper in preparation for transport 1 Control Time o oE Introduction to the Control Module Screens available from the System screen System Use the Esc key to receive Views on the F5 key Choose System from the Configuration pull down menu Use the F2 key Configure to select the ALS Here you define further special setpoints for the ALS operation uWucs 0 OOM Nore g mer ssreyy Options External Contacts Syringe Volume eas u Multiple Draw Wait 1 s Seat Capillary 2 3 8 pl On Missing Vial Abort H B o Options External Contacts pge Volume 100E ui Multiple Draw Wait 1 s Capillary 2 3 5
21. The stepper motor positions are determined by the optical encoders mounted onto the stepper motor housing The encoders monitor the position of the motors continually and correct for position errors automatically e g if the gripper is accidentally moved out of position when loading vials into the vial tray The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly 211 Introduction to the Autosampler Early Maintenance Feedback EMF Early Maintenance Feedback EMF Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user setable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The autosamplers provides two EMF counters Each counter increments with autosamplers use and can be assigned a maximum li
22. Using the Automated Needle Wash on page 41 the gripper arm replaces the sample vial positions the wash vial below the needle lowers the needle into the vial then lifts the needle out of the wash vial 9 The gripper arm checks the safety flap is in position 10 The gripper arm replaces the vial and returns to the home position Simultaneously the needle lowers into the seat 11 The injection valve switches to the mainpass position 204 Figure 31 Figure 32 Introduction to the Autosampler Sampling Sequence Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position Figure 34 In this position the mobile phase flows through the autosamplers metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over Mainpass Position 0 O Lee When the sample sequence begins the valve unit switches to the bypass position Figure 32 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 1 t Bypass Position 205 Figure 33 Figure 34 Introduction to the Autosampler Sampling Sequence Next the needle is raised and the vial is positioned below the needle The needle moves down into the vial and the metering unit draws the sample into the sample loop Figure 33 Drawing the Sample W
23. Valve Assy 2 Remove the three fixing screws 140 Repairing the Autosampler Injection Valve Assembly 4 Feed the cable of the new valve through to the 5 Connect the valve cable to the connector SUD board co labelled Valve Assy 6 Fix the valve assembly in place securely with the three screws On completion of this procedure 4 Install the sampling unit page 134 m 1 Install the top cover page 128 m 31 E Verify the transport assembly alignment page 99 Install the transport assembly page 132 Replace the injection valve capillaries page 141 Repairing the Autosampler Metering Drive Motor and Belt Metering Drive Motor and Belt Frequency When belt or motor defective Tools required Pozidrive No 1 screwdriver Parts required Metering drive motor 5062 8590 Belt 1500 0697 Before beginning this procedure 7 Disconnect the motor connector from the SUD board labelled Metering M Li Remove the top cover page 128 Li Remove the transport assembly page 132 Li Remove the sampling unit page 134 8 Remove the four fixing screws 142 Repairing the Autosampler Metering Drive Motor and Belt 10 Remove the belt 11 Place the belt over the shaft
24. because automatic electronic fuses are implemented in the power supply The thermostatted autosampler comprises two modules the autosampler and the thermostat G1330B Both modules have a separate power supply and a power plug for the line connections The two modules are connected by a control cable and both are turned on by the autosampler module To disconnect the autosampler from line power unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off To disconnect the thermostatted autosampler from line power unplug the power cord from the autosampler and the ALS thermostat The power supplies still use some power even if the power switch on the front panel is turned off Please make sure that it is always possible to access the power plug Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified 14 WARNING WARNING Installing the Autosampler Site Requirements Power Cords Your autosampler is delivered with a power cord which matches the wall socket of your particular country or region The plug on the power cord which connects to the rear of the instrument is identical for all types of power cord Never operate your instrumentation from a power outlet that has no ground connection Never use a power cord other than the power cord designed for your region Never use cabl
25. for example 2CHCI O gt gt 2COCl 2HCI This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF 284 Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1100 series modules for download 285 Agilent Technologies on Internet 286 Index A address switch 236 Agilent on internet 285 air circulation 15 analog signal output 232 Analytical 165 analytical head 208 APG remote interface 232 ASIC 220 ASM board 220 autosampler accessory kit contents 18 177 178 179 180 autosampler control 218 autosampler main board 220
26. pl On Missing Vial Abort cam Installed Trays Left Right 100 2 ml No tray 259 Introduction to the Control Module Screens available from the Records screen Screens available from the Records screen Records screen Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F4 key Records to select the ALS Errors are reported either into the System Log F2 or Error Log F3 uuc 0 O08GI Ready Serial Version On Time DE83100000 B 01 01 0d00 07h DE12345678 A 03 80 0d00 07h G1330A DE73300317 System Error Log Use the F2 key System Log or F3 key Error Log to look for errors For troubleshooting reasons they can be printed or saved to a file on the PCMCIA card pressing the m key uuc 0 008 Ready Message nitialization done 07 02 99 15 18 44 07 02 99 15 18 44 Records foes o OOF Ready Thermostat off Thermostat at setpoint 260 Info Log EMF Early Maintenance Feedback Introduction to the Control Module Screens available from the Records screen Use the m key to receive a pop up menu Select Info Log A list of the last events are listed For troubleshooting reasons they can be printed or saved to a file on the PCMCIA card pressing the m key Message Tray changed Info Ox00d9 10 External abort request On Error action executed Door closed 07 02 99 15 16 46 Use the F1 key EMF to set EMF
27. positions can be selected from the Maintenance menu in the Diagnosis display In the Control Module the functions can be selected in the Test screens of the autosampler User Interface The functions for the ChemStation and Control Module different names for functions in the Control Module are shown in brackets are Change Needle moves the safety flap away from the needle and positions the needle arm for easy access to the needle and needle seat Change Piston relieves the tension on the metering spring draws the piston to the outer position enabling easy disassembly of the metering head assembly Park Arm Park Gripper secures the gripper arm to the park position behind the sampling unit ready for transport or shipping of the autosampler Home moves the tray arm to its home position for better access and exchange of the trays Change Gripper The change gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism 80 WARNING NOTE Troubleshooting and Test Functions Change Needle Change Needle For needle exchange the needle arm moves down automatically when the front cover is removed To avoid personal injury keep fingers away from the needle area during needle movement The change needle seat function moves the safety flap out of position and positions the needle for easy exchange and alignment of the needle and needle s
28. signals from other modules Use the Right Left arrows to switch between Available and Selected Signals Use the F8 key Move to enter available signals into the box for selected signals or vice versa uuc 0 008 Ready elected signals Autosampler Auxilary 1 Autosampler Air Temp i a a Of Autosampler Auxilary 2 Time Range min 256 Method screens Introduction to the Control Module Screens available from the Analysis screen Use ESC to return to the Analysis screen Use the F3 key Method to view the parameters in a method and F8 key Save As to save the method in the mdodule s The PC Card key is only active when a PCMCIA card is inserted in the control module SoCRmy ORE Inj uuc o OOM Neteyg mmm at 1 a Setting ig AATEST aL iMetable Use F2 key PC Card to save a method on a PCMCIA card Use the Right Left arrows to switch between PC Card and Instrument window Use the UP Down arrows to select the method Use the F7 F8 keys Copy to enter available signals into the box for selected signals or vice versa Press the m key to open the context sensitive menu IISDLO206 07 02 99 14 51 H end of method list 4 Print Director 2Restart 257 System screen System Control Introduction to the Control Module Screens available from the System screen Screens available from the System screen Use the Esc key to receive Views on the F5 key
29. 1 2 3 4 5 6 7 8 TEST BOOT 1 1 0 0 1 0 0 0 To return to normal operation set switches back to your GPIB or RS 232C configuration settings 240 Theory of Operation The Main Power Supply Assembly The Main Power Supply Assembly The main power supply comprises a closed assembly no onsite repair possibility The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 120 or 220 240 volts AC 10 and needs no manual setting 241 Theory of Operation The Main Power Supply Assembly Figure 45 Main Power Supply MPS Blockdiagram MPS 36V 36V not used supply line i 24V gt primary ann voltage circuits 24V 4 54 5V switching J 45V 3A regulator linear voltage gt n a regulators 5 3 242 Figure 46 Table 61 Theory of Operation The Main Power Supply Assembly To disconnect the instrument from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off No accessible hardware fuse is needed because the main power supply is safe against any short circuits or overload conditions on the output lines When overload conditions occur the power supply turns off all output
30. 1100 Series of Modules 1 Year Onsite GC LC UV Visible and LAS supplies and 90 Days Onsite accessories Columns and Consumables 90 Days Return to Agilent Gas Discharge and Tungsten Lamps 30 Days Return to Agilent Repairs performed on site by Agilent 90 Days Onsite This warranty may be modified in accordance with the law of your country Please consult your local Agilent office for the period of the warranty for shipping instructions and for the applicable wording of the local warranty i Warranty services are included as specified for Analytical products and options purchased concurrently provided customer is located within a Agilent defined travel area Agilent warranty service provides for 8 a m to 5 p m on site coverage Monday through Friday exclusive of Agilent holidays Columns and Consumables are warranted to be free from defects for a period of 90 days after shipment and will be replaced on a return to Agilent basis if unused Agilent repair warranty is limited to only the item repaired or replaced 278 WARNING Safety Information Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability fo
31. 28 tray cover and front cover 35 installing the tray cover front door 35 instrument status indicator 52 interface board BCD LAN 228 interfaces analog signal output 232 APG remote 232 CAN 232 GPIB 232 overview 231 RS 232C 235 internet 285 introduction to the autosampler 202 IR sensor 219 L LAN interface board 228 LAN cables 200 LAN interface board 229 low volume injections 46 low volume capillary kit 35 low voume capillary kit 183 M main board 220 mainpass 205 maintenance functions 50 80 change metering seal 83 change needle 81 message ignition without cover 64 metering device 46 207 metering seal 165 metering seal 900 ul 165 167 micro thermostatted autosampler accessory kit contents 20 microswitches 219 missing parts 17 multi draw option 202 N needle drive 207 208 numbering of vials 32 0 optimizing performance automated needle wash 46 bypassing the autosampler 35 delay volume 46 delay volume adjustment 46 early maintenan 46 inject valve seal 46 low volume capillary kit 35 46 minimizing delay volume 35 38 P park arm 84 park transport assembly 17 35 parts and materials 17 900 ul injection upgrade kit 183 accessory kit 178 als thermostat 162 analytical head assembly 165 analytical head assembly optional 900 microlitre 165 autosampler accessory kit 177 autosampler main assemblies 161 autosampler sampling unit assembly 163
32. G1373 29A Analytical head assembly 40 ul for G1389A Preparative head assembly 900 ul for G7313 29A P lt 200Bar Preparative head assembly 900 ul for G2260A P lt 400Bar Inj valve Anal head cap 160 mm 0 25 mm for G1313 29A Inj valve Anal head cap 200 mm 0 05 mm for G1389A 20 pl FS Inj valve Anal head cap 200 mm 0 10 mm for G1389A 100 pl FS Inj valve Prep head cap 160 mm 0 50 mm for G2260A Injection valve assembly for G1313 29A Injection valve assembly for G1389A Injection valve assembly for G2260A Leak sensor Waste tube injection valve assy 120 mm for G1373 29A G2260A Waste tube injection valve assy 120 mm 0 25 mm for G1389A Safety cover 61313 60008 61329 60008 61329 60018 62260 60008 61313 66503 1500 0697 5062 8590 01078 87302 61329 87302 61375 87303 61313 87303 9022 2133 01078 60003 61377 60013 61313 60007 G2260 60007 61313 87301 61375 87302 61375 87312 62258 87301 0101 0921 0101 1050 0101 1267 5061 3356 61313 87300 61377 87301 61329 44105 163 Table 18 Parts and Materials Sampling Unit Assembly Autosampler Sampling Unit Assembly Item Description Part Number 11 12 13 14 15 Needle seat assy 0 17 mm i d 2 3 yl for G1313 29A STANDARD Needle seat assy 0 12 mm i d 1 2 ul for G1313 29A Needle seat assy 0 10 mm i d 1 2 ul for G1389A STANDARD Needle seat assy 0 05 mm i d 0 3 ul for G7389A Needle seat assy 0 5
33. NC 4 Blue Shut down Low 00r NC 5 Pink Not J connected Mie NC 6 Yellow Power on High 4 Key 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 to 3394 Integrators Connector Pin Pin Active 01046 60210 3394 Agilent 1100 Signal Name TTL 9 1 White Digital ground O A NC 2 Brown Prepare run Low o F e 3 3 Gray Start Low So NC 4 Blue Shut down Low o 3 a NC 5 Pink Not le connected Q NC 6 Yellow Poweron High 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected 190 Parts and Materials Remote Cables NOTE START and STOP are connected via diodes to pin 3 of the 3394 connector 191 Parts and Materials Remote Cables Agilent 1100 to 3396A Integrators Connector Pin Pin Active 03394 60600 3394 Agilent 1100 Signal Name TTL 9 1 White Digital ground O m NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected QO NC 6 Yellow Poweron High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 to 3396 Series II 3395A Integrators Use the cable 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 192 Parts and Materials Remote Cables Agilent 110
34. Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD_ FC Switches 1 and 2 define which set of parameters for example for GPIB RS 232C and so on will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory In the non volatile memory the parameters are kept regardless of whether you turn the instrument off and on again They will be kept until the same 236 Table 54 Theory of Operation Setting the 8 bit Configuration Switch set of parameters is subsequently changed and power is reset All other previously stored configuration settings will still remain in the non volatile memory In this way you can store more than one set of parameters using the same 8 bit configuration switch twice for example for both GPIB and RS 232C GPIB Default Addresses If you just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses Default Addresses for Agilent Series 1100 Modules Module Address Binary Address Pump 22 00010110 FLD 23 00010111 VWD 24 00011000 Agilent 8453A 25 00011101 DAD MWD 26 00011010 Column 27 00011011 compartment Autosampler 28 00011100 RID 29 00011101 where 0 means that the switch is down and 1 means that the switch is up 237 Tab
35. The analysis was completed successfully and the timeout function switched off the pump as requested e Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Suggested Actions 1 Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required 54 Probable Causes Suggested Actions Troubleshooting and Test Functions Shutdown Shutdown An external instrument has generated a shut down signal on the remote line The autosampler continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message e Leak detected in an external instrument with a remote connection to the system e Shut down in an external instrument with a remote connection to the system e The degasser failed to generate sufficient vacuum for solvent degassing 1 Fix the leak in the external instrument before restarting the autosampler 4 Check external instruments for a shut down condition m Check the degasser for an error condition Refer to the Reference Manual for the Agilent 1100 Series degasser 55 Troubleshooting and Test Functions Remote Timeout Remote Timeout A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions e g a not ready condition during detect
36. Typically lt 0 1 lt 0 05 with external needle cleaning 0 2 50 cp 1 99 100 x 2 ml vials in 1 tray 40 x 2 ml vials in 2 tray 15 x 6 ml vials in tray Agilent vials only Typically 50 s depending on draw speed and injection volume 270 Table 63 Specifications Performance Specifications Performance Specifications Agilent 1100 Series Autosampler G1313A and Thermostatted Autosampler G1329A Valid when standard 900 pl metering head installed Type Pressure GLP features Communications Safety features Injection range Replicate injections Precision Minimum sample volume Carryover Sample viscosity range Sample capacity Injection cycle time Specification Operating range 0 20 MPa 0 200 bar 0 2950 psi Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 900 ul in 0 1 ul increments recommended 1 ul increments Up to 1800 ul with multiple draw hardware modification required 1 99 from one vial Typically lt 0 5 RSD of peak areas from 5 2000 ul Typically lt 1 RSD of peak areas from 2000 5000 ul Typically lt 3 RSD of peak areas from 1 5 ul 1 ul from 5 ul sample in 100 u
37. a tissue and remove all liquid from it e RESET the injector 43 NOTE NOTE Optimizing Performance Fast Injection Cycle and Low Delay Volume Fast Injection Cycle and Low Delay Volume Short injection cycle times for high sample througput is one of the main issues in analytical laboratories Shortening cycle time starts with e shortening column length e high flow rate e steep gradient Having optimized these parameters further reduction of cycle times can be obtained using the overlapped injection mode Overlapped Injection Mode In this process as soon as the sample has reached the column the injection valve is switched back to bypass and the next injection cycle starts but waits with switching to mainpass until the actual run is finished You gain the sample preparation time when using this process Switching the valve into the bypass position reduces the system delay volume the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory in narrow bore and micro bore HPLC Having the valve in bypass position can increase the carry over in the system The injection cycle times also depend on the injection volume In identically standard condition injecting 100 pl instead of 1 pl increase the injection time by approximately 8 sec In this case and if the vi
38. boards are sensitive to electrostatic discharge ESD In order to prevent damage always use an ESD strap supplied in the accessory kit when handling electronic boards and components Using the ESD Strap 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 14 Using the ESD Strap 105 Repairing the Autosampler Overview of Main Repair Procedures Overview of Main Repair Procedures Figure 15 Main Assemblies ASM board page 148 Transport assembly page 132 Metering seal see page 123 MUI K MMMM f KN MMMM MUU a Needle page 108 Rotor seal page 111 Power supply 7 Needle seat page 154 page 111 106 Table 16 Repairing the Autosampler Simple Repairs Simple Repairs The procedures described in this section can be done with the autosampler in place in the stack You will do some of these procedures on a more frequent basis Simple Repair Procedures Procedure Exchanging the needle assembly Exchanging the seat assembly Exchanging the rotor seal Exchanging the metering seal Typical Frequency When needle shows indication of 15 minutes damage or bloc
39. cables 185 cover parts 171 external tray 184 foam parts 172 injection valve assembly 168 leak system parts 174 main assemblies 160 maintenance kit 181 micro thermostatted autosampler acc kit 179 multi draw kit 182 power and status light pipes 173 preparative ALS acc kit 180 preparative head assembly 167 sheet metal kit 170 thermostatted autosampler accessory kit 178 transport assembly 160 162 tray cover parts 171 vial trays and tray base 175 parts identification cables LAN cables 200 performance specifications 270 autosampler 270 271 preparative autosampler 273 micro autosampler 272 physical specifications 16 17 power consideration 14 power cords 15 power requirements 14 power supply description 241 specifications 243 288 Index power supply indicator 52 preparative autosampler accessory kit contents 21 preparative head 208 R reflection sensor 219 repair procedures 106 repairs ASM board 148 exchanging internal parts 126 fan 146 gripper arm 123 injection valve assembly 140 leak sensor 157 main board ASM 148 main cover 127 metering plunger 120 metering seal 120 metering drive motor and belt 142 needle assembly 108 needle drive motor and belt 144 needle seat assembly 111 power supply 154 rotor seal 117 sampling unit 134 simple repairs 107 stator face 114 SUD board 152 top cover and foam 128 130 transport assembly 132 restart without
40. computer through an RS 232 connection using appropriate software 214 Introduction to the Autosampler Electrical Connections This connector needs to be activated by the configuration switch module next to the GPIB connector The software needs the appropriate drivers to support this communication See your software documentation for further information e The Thermostat Autosampler connection only on thermostatted autosampler is used for control signal transfer and synchronization of the two modules The cable must be installed for operation of the ALS thermostat WARNING DO NOT disconnect or reconnect the autosampler to ALS thermostat cable when the power cords are connected to either of the two modules This will damage the electronics of the modules e The power input socket accepts a line voltage of 100 120 or 220 240 volts AC 10 with a line frequency of 50 or 60 Hz There is no voltage selector on your autosamplers because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents that the autosamplers cover is taken off when line power is still connected e The interface board slot is used for external contacts BCD output and for future use 215 Introduction to the Autosampler Electrical Connections 216 Theory of Operation Theory of operat
41. cover 65 RS 232 cable kit to PC 199 RS 232C communication settings 238 interface 235 settings 238 S safety information on lithium batteries 282 sample trays 32 numbering of vial positions 33 sampling sequence 204 sampling unit 207 seals metering seal 165 167 sensors 219 shipping 17 35 84 simple repairs 107 site requirements 14 specifications 16 17 270 stack configuration 23 25 rear view 23 25 stator 209 status indicator 50 status indicators 51 step commands 89 step functions 50 stepper motor 208 storage 16 T temperature 16 270 thermostatted autosampler accessory kit contents 19 theta axis 210 Time Out 54 transport 35 84 transport assembly 210 transport mechanism 202 U unpacking the autosampler 17 URL 285 V valve capillaries 31 verifying the gripper position 89 vial contents temperature 270 vial numbering 32 vial racks 202 vial tray 35 vials 202 viscous samples 46 volume 38 WwW warranty responsibility of Agilent Technologies 278 services 278 statement 276 weight 15 X X axis 210 Z Z axis 210 289 Index 290 fe Agilent Technologies In This Book This manual contains technical reference information about the Agilent 1100 Series Standard Thermostatted Micro and and Preparative Autosamplers The manual describes the following e installing the autosamplers e optimizing performance
42. e optimizing performance e troubleshooting and test functions e repairing the autosampler e parts and materials e introduction to the autosampler e theory of operation and e control module screens Contents Installing the Autosampler Site requirements and installation of the autosampler and thermostatted autosampler 13 Site Requirements 14 Unpacking the Autosampler 17 Optimizing the Stack Configuration 22 Installing the Autosampler 26 Installing the Thermostatted Autosampler 28 Flow Connections 31 Sample Trays 32 Choice of Vials and Caps 35 Transporting the Autosampler 38 Optimizing Performance How to optimize the autosamplers to achieve best results 39 Optimization for Lowest Carry over 41 Fast Injection Cycle and Low Delay Volume 44 Precise Injection Volume 46 Choice of Rotor Seal 48 Troubleshooting and Test Functions The modules built in troubleshooting and test functions 49 Status Indicators 51 Power Supply Indicator 52 Instrument Status Indicator 52 Contents Error Messages 53 Timeout 54 Shutdown 55 Remote Timeout 56 Sychronization Lost 57 Leak 58 Leak Sensor Open 59 Leak Sensor Short 60 Compensation Sensor Open 61 Compensation Sensor Short 62 Fan Failed 63 Open Cover 64 Restart Without Cover 65 Arm Movement Failed 66 Valve to Bypass Failed 67 Valve to Mainpass Failed 68 Needle Up Failed 69 Needle Down Failed 70 Missing Vial 71 Initialization Failed 72 Meteri
43. end positions No valve adjustments are required after replacing internal components Injection Valve Technical Data Standard Micro Preparative MBB Motor type Seal material Stator material Number of ports Switching time 4V 1 2A stepper motor Vespel Tefzel available Ceramic PEEK 6 lt 150 ms 4V 1 2A stepper motor Vespel Head coated SST 6 lt 150 ms 4V 1 2A stepper motor PEEK PEEK 6 lt 150 ms 209 Figure 36 X axis Theta axis Flex board Introduction to the Autosampler Transport Assembly Transport Assembly The transport unit comprises of an X axis slide left right motion a Z axis arm up down motion and a gripper assembly rotation and vial gripping Transport Assembly Theta motor Gripper motor The transport assembly uses four stepper motors driven in closed loop mode for accurate positioning or the gripper assembly for sample vial transport The rotational movement of the motors is converted to linear motion X and Z axes by toothed belts connected to the drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly 210 Introduction to the Autosampler Transport Assembly
44. functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 283 Solvent Information Solvent Information Observe the following recommendations on the use of solvents Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids
45. further subjected to decreasing pressure If the mobile phase is not adequately degassed small gas bubbles may form in the sample loop during the injection sequence When using injection volumes lt 2 pl these gas bubbles may affect the injection volume precision For best injection volume precision with injection volumes lt 2 pl use of the Agilent 1100 Series degasser is recommended to ensure the mobile phase is adequately degassed Also using the automated needle wash see Optimization for Lowest Carry over on page 41 between injections reduces carry over to a minimum improving injection volume precision further Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may have an influence on the injection volume precision when using viscous samples If the draw speed is too high air bubbles may form in the sample plug affecting precision The default draw speed is 200 pl min for the autosampler 4 pl min for the micro autosampler and 1000 l min for the preparative autosampler This speed is suitable for the majority of applications however when using viscous samples set the draw speed to lower speed for optimum results A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler Eject Speed The default eject speed setting is 200 l min for the autosampler 10 pl min for the micro autosampler and 1000 l min for the prepa
46. instrument from the line and unplug the power cord before maintenance Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Use the instrument only with Agilent CAN connected instrument or with remote controlled instrument Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Safety Symbols Table 67 shows safety symbols used on the instrument and in the manuals Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the Z instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected conductor terminal O Eye damage may result from directly viewing the light produced by the Xenon flash lamp used in this product Always turn the xenon flash lamp off before removing it 280 Safety Information WARNING A warning alerts you to situations that could cause physical injury or damage to the equipment Do not proc
47. mm above the seat 112 Repairing the Autosampler Needle Seat Assembly 6 Ensure the needle is aligned with the seat If On completion of this procedure required bend the needle slightly until the needle is aligned correctly 4 Install the front cover 1 Select End in the maintenance function Change Needle see Change Needle on page 81 113 Repairing the Autosampler Stator Face Stator Face Frequency Poor injection volume reproducibility Leaking injection valve Tools required 1 4 inch wrench supplied in accessory kit Hex key 9 64 inch supplied in accessory kit Parts required Stator face 0100 1851 for G1313A and G1329A Stator face 0101 1268 for G2260A CAUTION The stator face is held in place by the stator head When removing the stator head ensure the stator face does not fall out of the valve Before beginning this procedure 1 Remove all capillary fittings from the injection valve ports Li Remove the front cover i 114 Repairing the Autosampler Stator Face 2 Loosen each fixing bolt two turns at a time 3 Remove the stator head and stator face Remove the bolts from the head 4 Place the stator face in place on the stator 5 Install stator head and stator face Tighten the head Ensure the pins on the stat
48. module for further detailed reference Major keys on the Agilent 1100 Control Module ESC Return to previous screen and scroll through top layer views Analysis Settings m Open context sensitive menus i Information help Enter Store changed parameters or execute the choice from a pull down menu On Off Switch on lamp s Start Start a run Plot View the chromatogram Views Change between view of analysis status system screens The screens shown on the next pages are based on Control Module firmware revision B 01 01 LC Module firmware revision 3 8x In case the control module s display seems to be frozen hang up due to a communication problem on the CAN bus unplug the control module from the LC module and reconnect 246 Introduction to the Control Module Screens available from the Analysis screen Screens available from the Analysis screen The Analysis screen This is the wake up screen if the Agilent 1100 Series autosampler or thermostatted autosampler is the only configured Agilent 1100 module It is used to enter the most common ALS method parameters SSMA OREI Offed oiua o ooie Ready Inj Vol pl Draw Speed pl min Temp 4j c Eject Speed pl min 17 5 C Draw Position Offset mm 247 Introduction to the Control Module Screens available from the Analysis screen The m key allows access to the context sensitive menu Setup view leads you to add sections for additional Agilent 1100
49. modules Restart re boots the control module SSMA OREI Offfed oiua o ooie Ready AATEST peed pl min 1 Temp On Off x ARET Draw Speed pl min 3Reset 4Status 3 Eject Speed l min 5 Setup View 0 Draw Position Offset mm 6 Restart 248 Introduction to the Control Module Screens available from the Analysis screen In the Setup view e g the quaternary pump can be added to the view Here e g the quaternary pump parameters are shown on the display as well The number of parameters on the display are restricted as additional modules are added A maximum of 4 modules are shown automatically If more modules are in the system you have to chose in Setup view The number of parameters on the display are restricted as additional modules are added The m key gives access to a context sensitive menu e g when the cursor is positioned in the excitation window Fri 44 30 aX p Rea Oh TE asasi earner EN B Flow R Speed ith C 0 000 _ Draw 200 D OFF HA 30 0 249 Introduction to the Control Module Screens available from the Analysis screen With the Settings key you open a pull down menu where you can select the ALS module SSMA OREI Offed oiua o ooie Ready Draw Speed Eject Speed pl min Draw Position Offset mm Settings Within the Settings you can change the ALS parameters and with a different set of parameters available
50. naming conventions are xxxx vvv DLB where xxxx is the product number e g 1313 for the G1313A Autosampler and vvv is the revision number for example 380 is revision 3 80 For instructions refer to your user interface Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Firmware Update Mechanism Resident System Main FW update Resident FW update Main System 227 Table 47 Theory of Operation Optional Interface Boards Optional Interface Boards The Agilent 1100 Series modules have one optional board slot that allows addition of an interface board to the modules Optional Interface Boards Description Part Number BCD Board G1351 68701 Fuse 250 mA four are on the board 2110 0004 LAN Board see next page for details BCD Board The BCD board provides a BCD output for the bottle number of the Agilent 1100 Series autosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused There are general purpose cables available to connect the BCD output see BCD Cables on page 195 and the external outputs see External Contact Cable on page 198 to external devices 228 Theory of Operation Optional Interface Boards Block Diagram BCD Board
51. remove the transport protection foam 28 Figure 8 Air channel adapter 10 WARNING 11 12 Installing the Autosampler Installing the Thermostatted Autosampler Place the Autosampler on top of the Thermostat Make sure that the Autosampler is correctly engaged in the Thermostat locks Place the air channel adapter into the autosampler tray base Make sure the adapter is fully pressed down This assures that the cold airstream from the Thermostat is correctly guided to the tray area of the Autosampler Air channel adapter Re install the tray Ensure the power switch on the front of the Autosampler is OFF and the power cables are disconnected Connect the cable between the Autosampler and the Thermostat see Figure 9 Do not disconnect or reconnect the autosampler to ALS thermostat cable when the power cords are connected to either of the two modules This will damage the electronics of the modules Connect the power cables to the power connectors Connect the CAN cable to the other Agilent 1100 modules 29 13 14 15 NOTE WARNING WARNING Figure 9 Relay Vial number Thermostat Autosampler cable Installing the Autosampler Installing the Thermostatted Autosampler If an Agilent Chemstation is the controller connect either 1 The GPIB cable to the detector 4 The LAN connector to the LAN interface Connect the APG remote cable optional for non Agilent
52. sensors are located Six light sensors are used for vial tray identification one for gripper decoding and three for decoding of the initialization position 222 Figure 38 Theory of Operation Autosampler Main Board ASM Transport unit control f Oo Motor Theta Drive Encoder Motor Gripper Drive X Encoder gt Via transport FPGA Wot unit flex board _ X Axis Drive Encoder Be Motor L A m N gt Z Axis Drive Encoder gt ASIC Reflection sensors on transport unit flex board Tray decoding 6 gripper decoding 1 initialization sensors 3 Sampling Unit Control Needle arm metering device and valve motors are driven by controlled pulse width modulation in the same way as the SGS L6506 see Transport Unit Control on page 221 The motors require fast speed but do not require precise position control Therefore a closed loop servo system is not required Commutation is done in FPGA logic The needle arm metering device and valve motors use SGS L6203 output drivers to deliver the higher currents required for fast movement or high torque The movement sensing of the valve motor is done by two microswitches Two reflection light sensors are used to detect the end positions of the needle arm One photo sensor is required to detect the home position of the metering device Two hall sensors detect correct closur
53. through the F1 5 keys F7 key resets the ALS to default values F8 opens a window to turn on the thermostat Settings TEA OR ONE OM 0 0 Ready Standard Injection A O with needle wash O Injector Program dD Default Standard Injection i CO with needle wash i Default O Injector Program Default wash vial u 250 Introduction to the Control Module Screens available from the Analysis screen Use the m key for the context sensitive menu The Status command pulls up a monitor screen displaying details of injections and the sample tray Reset will initialize the autosampler SSMA OREI Offed oiua 0 ooie injVol C 20 05 jecti i N with needle wash Default 2Reset fo Done RvaC2oo pd e A 22 Default A O with needle wash A Set O Injector Program EATA Default wash vial As el Selecting More and Settings pull down menu you can enter special ALS setpoints SSMA OREI Offfed oiua o ooie Ready Draw Speed pl min Eject Speed 200 pl min Draw Position Offset ea 0 0 mm 251 Introduction to the Control Module Screens available from the Analysis screen Selecting More and Optimization on the pull down menu you can enter different optimization modes ouc 0 O008G Ready Optimization TERE eee de l at 1 00 min elapsed runtime m always keep transport a arm next to last usad vial Settings Timetable With the F2 key Timetable you ca
54. through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable Causes e Defective leak sensor Suggested Actions 1 Exchange the leak sensor 60 Probable Causes Suggested Actions Troubleshooting and Test Functions Compensation Sensor Open Compensation Sensor Open The ambient compensation sensor NTC on the ASM board in the autosampler has failed open circuit The resistance across the temperature compensation sensor NTC on the ASM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated e Defective ASM board 1 Exchange the ASM board 61 Probable Causes Suggested Actions Troubleshooting and Test Functions Compensation Sensor Short Compensation Sensor Short The ambient compensation sensor NTC on the ASM board in the autosampler has failed short circuit The resistance across the temperature compensation sensor NTC on the ASM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls be
55. voltages Turning the line power off and on again resets the power supply to normal operation if the cause of the overload condition has been removed An over temperature sensor in the main power supply is used to turn off output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument off wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Main Power Supply Specifications Maximum 130 W Continuous output power Line Input 100 120 or 220 240 volts AC Wide ranging 10 line frequency of 50 60 Hz Output 1 24 V 4 5 A maximum total power consumption of 24 V and 36 V must not Output 2 36 V 2 5 A maximum exceed 107 W Output 3 5V 3A Output 4 15V 03A Output 5 15V 03A 243 Theory of Operation The Main Power Supply Assembly 244 Introduction to the Control Module Introduction to the screens available for operation of the Agilent 1100 Series autosamplers with the control module NOTE NOTE Introduction to the Control Module This chapter is intended to introduce an operator to the screens available for operation of the Agilent 1100 Series autosamplers with the Agilent 1100 Series control module Please use the manual of Control
56. when the gripper is positioned below the needle Before beginning this procedure 1 Select the Needle Down button until the needle screw is aligned with the hole in the C1 Select Start in the maintenance function safety cover Change Needle see Change Needle on page 81 aS ES 108 Repairing the Autosampler Needle Assembly 2 Remove the sample loop fitting from the 3 Loosen the fixing screw and lift out the needle fitting needle gt he ON ga 4 Select Needle Down Repeat the selection 5 Insert the new needle Align the needle in the until the needle arm reaches its lowest seat then tighten the screw firmly J Far i lo AK es L Lf y iI 7 N X 6 109 Repairing the Autosampler Needle Assembly 6 Reconnect the sample loop fitting to the needle fitting 7 Use Needle Up to lift the needle to a position approximately 2mm above the seat 8 Ensure the needle is aligned with the seat If required bend the needle slightly until the needle is aligned correctly On completion of this procedure m I E m Install the front cover Select
57. 0 mm i d 20 ul for G2260A Seat adapter Safety flap Flex board Needle assembly for G71313 87101 or G1313 87103 needle seat Needle assembly for G7329 87101 or G1329 87103 needle seat Needle assembly 900 ul loop capillary for G7373 87101 needle seat Needle assembly 900 ul loop capillary for G2260 87101 needle seat Clamp Kit includes needle clamp and 2 x clamp screw 61313 87101 61313 87103 61329 87101 61329 87103 G2260 87101 61313 43204 61313 44106 G1313 68715 61313 87201 61329 80001 61313 87202 62260 87201 G1313 68713 164 Table 19 Table 20 Parts and Materials Analytical Head Assembly Analytical Head Assembly Analytical Head Assembly 100 pl for G1313A G1329A Item Description Part Number Analytical head assembly includes items 1 6 01078 60003 1 Screws 0515 0850 2 Plunger assembly 5063 6586 3 Adapter 01078 23202 4 Support seal assembly 5001 3739 5 Metering seal pack of 2 5063 6589 6 Head body 01078 27710 Screw M5 60 mm lg for mounting of assembly 0515 2118 Analytical Head Assembly 40 pl for G1389A Item Description Part Number Micro Analytical head assembly 40 ul includes items 1 6 G1377 60013 1 Micro Plunger assembly 5064 8293 2 Screws 0515 0850 3 Adapter 01078 23202 4 Micro seal support assembly G1377 60002 5 Metering seal pack of 1 5022 2175 6 Head body G1377 27700 Screw M5 60 mm lg for mounting of assembly 0515 2118 1
58. 0 to 3396 Series III 3395B Integrators Connector Pin Pin Active 03396 61010 33XX Agilent 1100 Signal Name TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not i connected O NC 6 Yelow Poweron High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector Pin Pin Active 5061 3378 HP 1050 Agilent 1100 Signal Name TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 193 Parts and Materials Remote Cables Agilent 1100 to HP 1090 LC HP 1040 DAD or Signal Distribution Module Connector Pin Pin Active 01046 60202 HP 1090 Agilent 1100 Signal Name TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 8 P a 4 3 Gray Start Low 6 mS ote El a 4 Blue Shut down Low eee 8 5 Pink Not A 1 connected NC 6 Yellow Power on High 5 Key 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 to General Purpose
59. 1100 series instruments Turn ON power by pushing the button at the lower left hand side of the sampler The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF To disconnect the sampler from the line unplug the power cord The power supply still uses some power even if the power switch at the front of the panel is turned OFF To avoid personal injury keep fingers away from the needle area during Autosampler operation Do not attempt to insert or remove a vial or a plate when the needle is positioned Cable Connections ma 2 00000 Remote cenana D 30 Installing the Autosampler Flow Connections Flow Connections Preparation Parts required Sampler is installed in the LC system Parts from the Accessory kit WARNING NOTE Figure 10 When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Connect the pump outlet capillary to port 1 of
60. 13 44501 4 Leak tubing 120 mm 5062 2463 5 Leak funnel 5041 8388 reorder gives 5 m Figure 27 Leak System Parts 174 Table 31 Figure 28 Parts and Materials Vial Trays Vial Trays Autosampler Vial Trays and Tray Base Item Description Part Number 1 Tray for 100 x 2 ml vials G1313 44500 2 Halftray for 15 x 6 ml vials G1313 44503 3 Halftray for 40 x 2 ml vials G1313 44502 4 Spring G1313 09101 5 Spring stud 0570 1574 6 Tray base for G1313A includes items 4 5 G1313 60002 Vial Trays and Tray Base 175 Table 32 Figure 29 Parts and Materials Vial Trays Thermostatted Autosampler Vial Trays and Tray Base Item Description Part Number 1 Adapter air channel 61329 43200 2 Tray for 100 x 2 ml vials thermostattable G1329 60001 3 Spring 61313 09101 4 Tray base for G1329A G1389A G2260A 61329 60000 includes items 4 5 5 Spring stud 0570 1574 Thermostatted Autosampler Vial Trays and Tray Base 176 Table 33 Parts and Materials Autosampler Accessory Kit G1313 68705 Autosampler Accessory Kit G1313 68705 G1313A Autosampler Accessory Kit Contents G1313 68705 Description Part Number Tubing assembly Filter promo kit CAN cable Screw cap vials clear 100 pk Blue screw caps 100 pk Label halftray Vial instruction sheet Wrenches 1 4 5 16 inch Rheotool socket wrench 1 4 inch Hex key 4 mm 15 cm long T handle Hex
61. 52 Power Supply 154 Leak Sensor 157 Parts and Materials Detailed illustrations and lists for identification of parts and materials 159 Main Assemblies 160 Sampling Unit Assembly 162 Analytical Head Assembly 165 Injection Valve Assembly 168 Sheet Metal Kit 170 Cover Parts 171 Contents Foam Parts 172 Power and Status Light Pipes 173 Leak System Parts 174 Vial Trays 175 Autosampler Accessory Kit G1313 68705 177 Thermostatted Autosampler Accessory Kit G1329 68705 178 Micro Thermostatted Autosampler Accessory Kit G1329 68715 179 Preparative ALS Accessory Kit G2260 68705 180 Maintenance Kit G1313 68709 181 Multi Draw Kit G1313 68711 182 900 ul Injection Upgrade Kit G1363A for G1313A 29A 183 External Tray G1313 60004 184 Cable Overview 185 Analog Cables 187 Remote Cables 189 BCD Cables 195 Auxiliary Cable 197 CAN Cable 197 External Contact Cable 198 RS 232 Cable Kit 199 LAN Cables 200 Introduction to the Autosampler An introduction to the standard micro and preparative au tosamplers and thermostatted autosamplers 201 Introduction to the Autosampler 202 Sampling Sequence 204 Contents Sampling Unit 207 Transport Assembly 210 Early Maintenance Feedback EMF 212 Electrical Connections 214 Theory of Operation Theory of operation of mechanical hardware electronics and instrument interfaces 217 Autosampler Control and Electronics 218 Position and Movement Sensors 219 Autosample
62. 65 Parts and Materials Analytical Head Assembly Figure 21 Analytical Head Assembly 166 Parts and Materials Analytical Head Assembly Table 21 Preparative Head Assembly 900 pl for G1313A G1329A Item Description Part Number Analytical head assembly 900 ul includes items 1 6 61313 60007 1 Plunger assembly 900 ul 5062 8587 2 Screws 0515 0850 3 Adapter 01078 23202 4 Support seal assembly 900 pl 5001 3764 5 Metering seal 900 pl 0905 1294 6 Head body 900 ul G1313 27700 Screw M5 60 mm lg for mounting of assembly 0515 2118 This head is limited to 200 Bars Table 22 Preparative Head Assembly 900 pl for G2260A Item Description Part Number Analytical head assembly 900 ul includes items 1 6 G2260 60007 1 Plunger assembly 900 yl 5062 8587 2 Screws 0515 0850 3 Adapter 01078 23202 4 Support seal assembly 900 pl 5001 3764 5 Metering seal 900 pl 0905 1294 6 Head body 900 ul G2260 27700 Screw M5 60 mm lg for mounting of assembly 0515 2118 This head is limited to 400 Bars It can only be assembled on a sampling unit with the description supports 900 pl at 400 Bar 167 Table 23 Table 24 Parts and Materials Injection Valve Assembly Injection Valve Assembly Injection Valve Assembly for G1313A G1329A Item Description Part Number 1 Injection valve assembly includes items1 6 Isolation seal Rotor seal Vespel Rotor seal Tef
63. Autosampler Unpacking the Autosampler Unpacking the Autosampler If you need to ship the autosampler at a later date always park the transport assembly before shipment see Transporting the Autosampler on page 38 Damaged Packaging Upon receipt of your autosampler inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the autosampler has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection If there are signs of damage to the autosampler please do not attempt to install the autosampler Delivery Checklist Ensure all parts and materials have been delivered with the autosampler The instrument box contains the instrument and an Accessory kit A separate box contains the reference manual and the power cable In Table 3 Table 4 Table 5 and Table 6 are listed the content of each accessory kit Please report missing or damaged parts to your local Agilent Technologies sales and service office 17 Table 2 Installing the Autosampler Unpacking the Autosampler G1313A Autosampler Accessory Kit Contents G1313 68705 Description Part Number Tubing assembly 5063 6527 Filter promo kit 5064 8240 CAN cable 5181 1516 Screw cap vials clear 100 pk 5182 0714 Bl
64. Black 5 BCD 0 1 gt Orange 6 BCD 3 8 a Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground White 15 5 Vt Low 196 Parts and Materials Auxiliary Cable Auxiliary Cable CE One end of this cable provides a modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Agilent 1100 Series Degasser to general purposes Connector Pin G1322 81600 Color Agilent 1100 Signal Name White 1 Ground Brown 2 Pressure signal _ Green 3 a Yellow 4 a Grey 5 DC 5VIN Pink 6 Vent CAN Cable im Both ends of this cable provide a modular plug to be connected to Agilent 1100 Series module s CAN bus connectors Agilent 1100 module to module 0 5m Ig 5181 1516 Agilent 1100 module to module 1m Ig 5181 1519 Agilent 1100 module to control module 61323 81600 197 Parts and Materials External Contact Cable External Contact Cable One end of this cable provides a 15 pin plug to be connected to Agilent 1100 Series module s interface board The other end is for general purpose Agilent 1100 Series Interface Board to general purposes Connector Pin G1103 61611 Color Agilent 1100 Signal Name White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Pn Red 8 EXT 4 P Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not co
65. End in the maintenance function Change Needle see Change Needle on page 81 110 Repairing the Autosampler Needle Seat Assembly Needle Seat Assembly Frequency When the seat is visibly damaged When the seat capillary is blocked Tools required 1 4 inch wrench supplied in accessory kit Flat head screwdriver Parts required Needle seat assy 0 17 mm i d 2 3 ul G1313 87101 for G1313 29A Needle seat assy 0 12 mm i d 1 2 ul G1313 87103 for G1313 29A Needle seat assy 0 10 mm i d 1 2 ul G1329 87101 for G1389A Needle seat assy 0 05 mm i d 0 3 ul G1329 87103 for G1389A Needle seat assy 0 50 mm i d 20 ul G2260 87101 for G2260A Before beginning this procedure 1 Disconnect the seat capillary fitting from the injection valve port 5 1 Select Start in the maintenance function Change Needle see Change Needle on page 81 L Remove the front cover Ll Use the Needle Up command in the Change Needle function to lift the needle an addition lcm 111 Repairing the Autosampler Needle Seat Assembly 2 Use a small flat head screwdriver to ease out 3 Insert the new needle seat assembly Press the needle seat the seat firmly into position aN YN 4 Connect the seat capillary fitting to port 5 of 5 Use the Down button to position the needle the injection valve approximately 2
66. Flap Install the LAN interface board in the sampler if required see Agilent 1100 Series Interface Board to general purposes on page 198 Remove the adhesive tape which covers the front door Remove the front door and remove the transport protection foam Place the Autosampler on the bench or in the stack as recommended in Recommended Stack Configuration for an Autosampler Front View on page 22 26 orm A 10 Figure 6 CAN cable to previous module Installing the Autosampler Installing the Autosampler Ensure the power switch at the front of the Autosampler is OFF Connect the power cable to the power connector at the rear of the sampler Connect the CAN cable to the other Agilent 1100 modules If an Agilent Chemstation is the controller connect either 1 The GPIB cable to the detector 1 The LAN connector to the LAN interface Connect the APG remote cable optional for non Agilent 1100 series instruments Turn ON power by pushing the button at the lower left hand side of the sampler Cable Connections Remote GPIB RS232C NOTE NOTE WARNING ia CAN bus If the front cover is not installed the autosampler is in a not ready condition and operation is inhibited The sampler is turned ON
67. For reliable operation vials used with the 1100 Autosampler must not have tapered shoulders or caps that are wider than the body of the vial For more details see the service note G1313 017 Very heavy usage use a macro A pre sequence macro OMBUVHW_PDF will automatically reset the sampler at the start of a sequence ChemStation Check if the INJECT line is used in the Injector Program Remove this line from the program In this mode the system does not need this command to do the injection A firmware revision gt 3 81 will address this problem For more details see the service note G1313 018 Reset the sampler alignment to default value Reset tray alignment and transport alignment is possible with the Control Module and the ChemStation To reset the transport alignment with the ChemStation enter following command in the command line Print sendmodule lals tray alig 0 00 0 00 95 Step 6 NOTE Step 7 Step 8 Step 9 Step 10 Troubleshooting and Test Functions Intermittent lock ups with or without vial in the gripper fingers Check the tension of the belts For this use the Torque2 mac and measure the torque for each axis Typical ranges Theta both 30 50 X axis both 50 90 Z axis both 90 130 Gripper open 30 65 Gripper closed maximum 30 If the Gripper open closed torque is not in the range proceed with STEP 7 If the theta or X torque is not in the range proceed wi
68. G2260A Power supply assembly part not shown Screw M4 8 mm lg power supply part not shown BCD board not shown Cable autosampler to ALS thermostat part not shown 61313 60009 61329 60009 61313 60008 61329 60008 61329 60018 62260 60008 01078 60003 61377 60013 61313 60007 62260 60007 0101 0921 0101 1050 0101 1267 G1329 60001 G1313 60010 61313 69520 61329 69500 0380 0643 1251 7788 G1313 81601 G1313 81602 01090 87306 61375 87304 61375 87311 62260 87300 0950 2528 0515 0910 G1351 68701 G1330 81600 161 Parts and Materials Sampling Unit Assembly Sampling Unit Assembly Figure 20 Autosampler Sampling Unit Assembly 162 Table 18 Parts and Materials Sampling Unit Assembly Autosampler Sampling Unit Assembly Item Description Part Number me a wo N Sampling unit assembly for G1313A Sampling unit assembly for G1329A Sampling unit assembly for G1389A Sampling unit assembly for G2260A The assy comes without injection valve and analytical head Sampling unit connector board SUD Belt gear for metering unit and needle arm Stepper motor for metering unit and needle arm Loop capillary 100 ul for G1313 29A G2260A Loop capillary 40 ul for G1389A Loop capillary 8 ul for G1389A Loop ext capillary 900 ul for G1313 29A G2260A Union for 900 pl loop extension capillary Analytical head assembly 100 ul for
69. Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel adapter 61329 43200 Tray for 15 x 6 ml vials x2 G1313 44503 Union loop extension 5022 2133 Seat extension capillary 500 pl G1313 87307 Seat extension capillary 1500 ul 61313 87308 Sampler Column capillary G2260 87300 Pump Sampler capillary G2260 87301 180 Table 37 Parts and Materials Maintenance Kit G1313 68709 Maintenance Kit G1313 68709 Maintenance Kit Item Description Part Number 1 Rotor seal Vespel 0100 1853 2 Needle assembly 61313 87201 3 Needle seat assembly 61313 87101 4 Metering seal pack of 2 5063 6589 5 Finger caps pack of 15 5063 6506 181 Table 38 Parts and Materials Multi Draw Kit G1313 68711 Multi Draw Kit G1313 68711 Multi Draw Kit Item Description Part Number 1 2 3 Seat capillary 500 ul 0 5 mm id Seat capillary 1500 pl 0 9 mm id Union 61313 87307 61313 87308 0100 0900 182 Table 39 Parts and Materials 900 ul Injection Upgrade Kit G1363A for G1313A 29A 900 ul Injection Upgrade Kit G1363A for G1313A 29A 900 pl Injection Upgrade Kit Item Description Part Number 1 gt wo N Analytical Head 900 ul Loop Extension 900 ul Union loop extension Needle 900
70. RANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE Limitation of Remedies and Liability THE REMEDIES PROVIDED HEREIN ARE BUYER S SOLE AND EXCLUSIVE REMEDIES IN NO EVENT SHALL AGILENT BE LIABLE FOR DIRECT INDIRECT SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES INCLUDING LOSS OF PROFITS WHETHER BASED ON CONTRACT TORT OR ANY OTHER LEGAL THEORY Responsibilities of the Customer The customer shall provide 1 access to the products during the specified periods of coverage to perform maintenance 2 adequate working space around the products for servicing by Agilent personnel 3 access to and use of all information and facilities determined necessary by Agilent to service and or maintain the products insofar as these items may contain proprietary or classified information the customer shall assume full responsibility for safeguarding and protection from wrongful use 4 routine operator maintenance and cleaning as specified in the Agilent operating and service manuals and 5 consumables such as paper disks magnetic tapes ribbons inks pens gases solvents columns syringes lamps septa needles filters frits fuses seals detector flow cell windows and so on 277 Table 66 Warranty Statement Responsibilities of Agilent Technologies Agilent Technologies will provide warranty services as described in Table 66 Warranty Services Services During Warranty Warranty Period Type Agilent
71. Rear View GPIB or LAN to LC ChemStation Analog signal to recorder Autosampler Thermostat cable Remote cable Analog signal to recorder AC power CAN bus cable AC power 25 Installing the Autosampler Installing the Autosampler Installing the Autosampler Preparation Parts required Locate bench space Provide power connection Unpack the Sampler Sampler Power cord for the other cables see below and Cable Overview on page 185 Chemstation and or Control Module G1323B WARNING Figure 5 Safety cover Safety flap To avoid personal injury keep fingers away from the needle area during autosampler operation Do not bend the safety flap away from its position or attempt to remove the safety cover see Figure 5 Do not attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle Safety
72. Settings Thermostat With the F4 key Thermostat you can set the autosampler thermostat temperature Settings FEA OR ODE OM o oo Ready Temperature C 9 6 C O at Power On turn temperature c on a aa aaa a Done _ Settings Run times With the F5 key Runtimes you can change the stop time and the post run time SSA OREI Offed oiua 0 O008GI Ready Stoptime 0 00 min Posttime OFF 0 00 min E overrides HPLC system runtime settings r 254 Introduction to the Control Module Screens available from the Analysis screen Status Press F5 key Views and select Status SSMA OREI Oped oiua 0 ooie Ready Vial 1 E p Installed Trays Injection 1 100 2ml No tray Wes l Elapsed 0 10 min Signal plot Press F6 key Plot to enter the plot screen available also from the Analysis and System screen Here you can observe the on line signal s To add additional on line signals maximum 3 press F6 key Select If more than one signal is configured see next use the 1 2 3 number key to switch between the signals 255 Introduction to the Control Module Screens available from the Analysis screen Press the m key to open the context sensitive menu uuc 0 008 Ready 9 0 C 1Clear All 2Print Plot 3Maximize Press F6 key Select Here you can add additional on line signals maximum are 3 Additional signals could be also pressure or temperature
73. Signal Name 1 Not connected 2 Shield Analog 3 Center Analog stata 187 Parts and Materials Analog Cables Agilent 1100 to BNC Connector Connector Pin Pin 8120 1840 BNC Agilent 1100 Signal Name Shield Shield Analog Center Center Analog ST G T BHO Agilent 1100 to General Purpose Connector Pin Pin 01046 60105 3394 6 Agilent 1100 Signal Name 1 Not connected 2 Black Analog BS 3 Red Analog L f e 188 Parts and Materials Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1100 Series modules The other end depends on the instrument to be connected to Agilent 1100 to 3390 Integrators Connector Pin Pin Active 01046 60203 3390 Agilent 1100 Signal Name TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low i a NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low 189 Parts and Materials Remote Cables Agilent 1100 to 3392 3 Integrators Connector Pin Pin Active 01046 60206 3392 3 Agilent 1100 Signal Name TTL 3 1 White Digital ground oN J NC 2 Brown Prepare run Low 11 3 Gray Start Low 00
74. Step Failures continued Step Function Probable Failure Modes Draw Sum of all draw volumes exceeds 100ul Defective metering drive motor Needle Up Needle already in the upper position Needle already in the upper position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Vial to Tray Defective transport assembly motors Sticking transport assembly Defective gripper assembly Gripper not aligned see page 87 Needle Down Needle already in the lower position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Mainpass Valve already in mainpass Valve not connected Defective injection valve Needle Up Mainpass Blockage in the sample loop or needle no solvent flow Needle already in the upper position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Valve already in mainpass Valve not connected Defective injection valve 92 Troubleshooting and Test Functions Step Commands 93 NOTE Troubleshooting and Test Functions Troubleshooting Guide for the Sample Transport Assembly Troubleshooting Guide for the Sample Transport Assembly This troubleshooting guide is meant to help you diagnose and repair autosampler problems In general autosampler problems can be divided into three catego
75. ad stator ring 4 Remove the rotor seal and isolation seal if 5 Install the new rotor seal and isolation seal if required required Ensure the metal spring inside the isolation seal faces towards the valve body 118 Repairing the Autosampler Rotor Seal 6 Install the stator ring Ensure the housing is flush against the valve body 7 Place the stator face in place on the stator head 8 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure 9 Reconnect the pump capillaries to the valve ports see Hydraulic Connections on page 31 JU 10 Slide the waste tube into the waste holder in the leak tray On completion of this procedure 1 Install the front cover 119 Repairing the Autosampler Metering Seal and Plunger Metering Seal and Plunger Frequency Poor injection volume reproducibility Leaking metering device Tools required 1 4 inch wrench supplied in accessory kit 4 mm hex key supplied in accessory kit 3 mm hex key supplied in accessory kit Parts required Metering seal 5063 6589 pack of 2 for 100 pl analytical head Metering seal 5022 2175 pack of 1 for 40 pl analytical head Metering seal 0905 1294 pack of 1 for 900 pl analytical head Metering plunger 5063 6586 for 700 pl analytical head Metering
76. also be seen by typing the following command into the command line print sendmodule lals ser The reply line will give the module serial number Replacing the Autosampler Firmware The installation of new firmware is required e if new version solves problems of currently installed version e if after exchange of the mainboard ASM the version on board is older than previous installed one To upgrade the autosampler firmware the following steps have to be performed 1 Load the firmware into the autosampler see the help system of your user interface 151 Repairing the Autosampler SUD Board SUD Board Frequency When defective Tools required Pozidrive No 1 screwdriver Parts required SUD board G1313 66503 CAUTION Take care not to damage the flex board when removing the SUD board Ce er a al Before beginning this procedure Li Remove the top cover page 128 Li Remove the transport assembly page 132 Li Remove the sampling unit page 134 1 Disconnect all connectors from the SUD board 2 Remove the two fixing screws and lift out the board 3 Install the new board Ensure the board is positioned between the board guide Fixing screws 152 Repairing the Autosampler SUD Board 4 Secure the board in place with the two screws 5 Reconnect all the connectors to the new Fixing screws board AA
77. ample tray the processor monitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated e No vial in the position defined in the method or sequence e Incorrect gripper alignment e Defective gripper assembly defective gripper fingers or belt e Defective transport assembly flex board 1 Install the sample vial in the correct position or edit the method or sequence accordingly LI Align gripper E Exchange the gripper assembly 41 Exchange the transport assembly 71 Probable Causes Suggested Actions Troubleshooting and Test Functions Initialization Failed Initialization Failed The autosampler failed to complete initialization correctly The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined sequence During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Mechanical obstruction Defective sampling unit flex board Defective transport assembly flex board Defective sampling unit motor Defective ASM board Ensure unobstructed movement of the transport assembl
78. and the Chemstation To reset the transport alignment with the Chemstation enter following command in the command line Print sendmodule lals tray alig 0 00 0 00 Lubricate the X gear Friction can result in the belt slipping on the gear so that the position of the belt teeth towards the gear changes To avoid this apply some grease from the sample transport repair kit to the X motor gear Do not use other grease as the one in the kit and carefully follow the instruction from the technical note 99 Step 4 NOTE Step 5 Step 6 Step 7 Step 8 Troubleshooting and Test Functions Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm Check the tension of the belts For this use the Torque2 mac and measure the torque for each axis Typical ranges Theta both 30 50 X axis both 50 90 Z axis both 90 130 Gripper open 30 65 Gripper closed maximum 30 If the Gripper open closed torque is not in the range proceed with STEP 5 If the theta or X torque is not in the range proceed with STEP 6 if you think you can adjust the torque otherwise proceed with STEP 7 Exchange the gripper arm assembly part number G1313 60010 The gripper arm exchange procedure is explained in the reference manual G1313 90004 section Repairing the Autosampler Adjust the belts tension If the measured torque value is too low the belt needs to be tight
79. arts and Materials Main Assemblies Main Assemblies Figure 19 Autosampler Main Assemblies LOOOL LOUR LLL J GOOOLL BUUU GR ff Ubddidddidddiddd JS 160 Table 17 Parts and Materials Main Assemblies Autosampler Main Assemblies Item Description Part Number 1 Transport assembly for G1313A Transport assembly for G1329A 1389A 2260A Sampling unit assembly for G1313A Sampling unit assembly for G1329A Sampling unit assembly for G1389A Sampling unit assembly for G2260A The assy comes without injection valve and analytical head Analytical head assembly 100 ul for G1373 29A Analytical head assembly 40 ul for G7389A Preparative head assembly 900 ul for G1313 29A P lt 200Bar Preparative head assembly 900 ul for G2260A P lt 400Bar Injection valve assembly for G1313 29A Injection valve assembly for G7389A Injection valve assembly for G2260A Vial tray thermostatted see page 175 Gripper assembly Autosampler Main Board ASM for G1373 Autosampler Main Board ASM for G1329A 1389A 2260A Standoff GPIB connector part not shown Standoff remote connector part not shown Ribbon cable sample transport Ribbon cable sampling unit Sampler TCC cap 380 mm 0 1 mm id for G1329A Sampler TCC cap 500 mm 0 05 mm id for G1389A 20 pl FS Sampler TCC cap 500 mm 0 075 mm id for G1389A 100 pl FS Sampler Column cap 600 mm 0 5 mm id for
80. axis are solved Check the cleanliness of the transport rods X axis and clean them If the rod is dirty or sticky clean it with Isopropanol and wipe it with a lint free cloth The rod can be lubricated with the following synthetic oil part number 6040 0854 DO NOT use other lubricant as mentioned above Lubricate the X gear Friction can result in the belt slipping on the gear so that the position of the belt teeth towards the gear changes To avoid this apply some grease from the sample transport repair kit to the X motor gear Do not use other grease as the one in the kit and carefully follow the instruction from the technical note 97 Step 4 Step 5 Step 6 Step7 Step 8 Troubleshooting and Test Functions Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the via Check the tension of the belts For this use the Macro2 mac and measure the torque for theta and X axis Typical ranges Theta both 30 50 X axis both 50 90 e Ifthe theta or X torque is not in the range proceed with STEP 5 if you think you can adjust the torque Otherwise proceed with STEP 7 Adjust the belts tension e Ifthe measured torque value is too low the belt needs to be tightened e Ifthe measured torque value is too high the belt needs to be loosened For this slide the motor X or theta on the holder bracket in the appropriate direction and test the ten
81. centration carry over may become noticeable Using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat Using the Automated Needle Wash The automated needle wash can be programmed either as injection with needle wash or the needle wash can be included into the injector program When the automated needle wash is used the needle is moved into a wash vial after the sample is drawn By washing the needle after drawing a sample the sample is removed from the surface of the needle immediately Uncapped Wash Vial For best results the wash vial should contain solvent in which the sample components are soluble and the vial should not be capped If the wash vial is capped small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample 41 Optimizing Performance Optimization for Lowest Carry over Injector Program with Needle Wash The injector program includes the command NEEDLE WASH When this command is included in the injector program the needle is lowered once into the specified wash vial before injection For example 1 DRAW 5 ul 2 NEEDLE WASH vial 7 3 INJECT Line 1 draws 5 pl from the current sample vial Line 2 moves the needle to vial 7 Line 3 injects the sample valve switches to main pass Using an Injector Program The process is based on a program that switches the bypass grove of the injection
82. d to the drive shaft by a toothed belt The drive nut on the spindle converts the circular movement of the spindle to linear motion The drive nut pushes the sapphire plunger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampling unit flex board while the sample volume is determined by counting the number of steps from the home position The backward movement of the plunger driven by the spring draws sample from the vial Analytical Head Technical Data Standard Micro 40 ul Standard Preparative 100 ul 900 pl 900 ul Number of steps 15000 60000 15000 15000 Volume resolution 7 nl motor step 0 7 nl motor step 60 nl motor step 60 nl motor step Maximum stroke 100 pl 40 ul 900 ul 900 ul Pressure limit 400 bars 400 bars 200 bars 400 bars Plunger material Sapphire Sapphire Sapphire Sapphire 208 Table 43 Introduction to the Autosampler Sampling Unit Injection Valve The two position 6 port injection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass
83. e home position Using the Change Seal Function 1 Select Start Change to move the gripper arm to the maintenance position 2 Exchange the gripper arm see Gripper Arm on page 123 3 Select End Done to move the gripper arm to the home position 85 Troubleshooting and Test Functions Change Gripper Change Arm NOTE When the autosampler is switched on again the arm is initialized and moves out of the park position to the home position automatically No other action is required to prepare the autosampler for use 86 NOTE WARNING Troubleshooting and Test Functions Tray Alignment Tray Alignment Tray alignment is required to compensate for small deviations in positioning of the gripper which may occur after disassembling the module for repair The tray alignment procedure uses several tray positions as reference points Because the tray is a rectangle a two point alignment is sufficient to corrects all other vial positions within the tray On completion of the procedure the corrected gripper positions are stored in the instrument firmware The alignment procedure requires an 1100 Control Module G1323B with firmware revision B 02 02 or higher The screen with the alignment dialog box can be found under the menus Views System Tests Autosampler The alignment procedure must be done with the standard 100 position vial tray installed The alignment procedure ha
84. e Lugs 18594 60520 Auxiliary Agilent 1100 Series vacuum degasser 61322 61600 CAN cables Agilent 1100 module to module 0 5m Ig 5181 1516 Agilent 1100 module to module 1m Ig 5181 1519 Agilent 1100 module to control module G1323 81600 External Agilent 1100 Series interface board to general purpose G1103 61611 contacts GPIB cable Agilent 1100 module to ChemStation 1 m 10833A Agilent 1100 module to ChemStation 2 m 10833B RS 232 Agilent 1100 module to a computer 34398A cable This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter LAN cable Twisted pair cross over LAN cable 10 feet long 5183 4649 for point to point connection Category 5 UTP cable 8 m long G1530 61480 for hub connections 186 Parts and Materials Analog Cables SH Analog Cables One end of these cables provides a BNC connector to be connected to Agilent 1100 Series modules The other end depends on the instrument to which connection is being made Agilent 1100 to 3390 2 3 Integrators Connector Pin Pin 01040 60101 3390 2 3 Agilent 1100 Signal Name 1 Shield Ground 2 Not connected 5 F 4 3 Center Signal 4 Connected to pin 6 TRS 2 BY J 4 5 Shield Analog 3 BRN i BRN 6 Connected to pin 4 7 Key 8 Not connected Agilent 1100 to 3394 6 Integrators Connector Pin Pin 35900 60750 3394 6 Agilent 1100
85. e of the door needle arm movement is interrupted if the door is open All the sensors are mounted on one flex board The flex board and motors are connected to the sampling unit distribution board SUD The SUD board is connected to the autosampler main board ASM via a flat band cable 64 pin 223 Figure 39 Theory of Operation Autosampler Main Board ASM Sampling unit control Sampler Main Board FPGA Needle Drive 2A Phase Needle ia End Position Sensors 2 Metering Drive 2A Phase Metering Home Position Sensor Valve Drive 4 5A Phase Safety Hall Sensors 2 a Mixing Drive 0 6A Phase Via sampling unit flex board Sampling Unit Distribution Board Sensor Circuits aN ES Valve End Position Sensors 2 224 Figure 40 Theory of Operation Autosampler Main Board ASM Autosampler block diagram Limit switch signals 1 0 signals lt 4 lt gt gt Core common Resources gt TPU Injection signals o i gt Valve gt 8 bit data lt gt 5 bit Metering address gt Device FPGA chi ea gt Sampling select p Unit L p Encoder gt clocks and direction m gt o 5 bit 48 bit data address Serial data lt
86. e subjected to excessive shock Also ensure the vial tray is secured in place with suitable packaging 38 Optimizing Performance How to optimize the autosamplers to achieve best results Optimizing Performance Autosamplers are more and more used in HPLC to improve the productivity in the laboratories and the consistency and accuracy of analytical results The informations below will help you on how to optimize some parameters to achieve best results for e lowest carry over for reliable quantitative data e Fast injection cycles for high throughput e Low delay volume for fast gradient e Precise injection volume 40 8 16 01 AUTHOR S DRAFT Optimizing Performance Optimization for Lowest Carry over Optimization for Lowest Carry over Several parts of an injection system can contribute to carry over e needle outside e needle inside e needle seat e sample loop e seat capillary e injection valve The autosampler continuous flow through design ensures that sample loop needle inside seat capillary and the mainpass of the injection valve is always in the flow line These parts are continuously flushed during an isocratic and also during a gradient analysis The residual amount of sample remaining on the outside of the needle after injection may contribute to carry over in some instances When using small injection volumes or when injecting samples of low concentration immediately after samples of high con
87. eak Leak A leak was detected in the autosampler The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the ASM board Probable Causes e Loose fittings e Broken capillary e Leaking rotor seal or needle seat e Defective metering seal Suggested Actions Ensure all fittings are tight Exchange defective capillaries Exchange the rotor seal or seat capillary Ooco Exchange the metering seal 58 Probable Causes Suggested Actions Troubleshooting and Test Functions Leak Sensor Open Leak Sensor Open The leak sensor in the autosampler has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated e Leak sensor not connected to the ASM board e Defective leak sensor 1 Ensure the leak sensor is connected correctly 1 Exchange the leak sensor 59 Troubleshooting and Test Functions Leak Sensor Short Leak Sensor Short The leak sensor in the autosampler has failed short circuit The current
88. eat User Interface The commands for the ChemStation and Control Module different names for the commands in the Control Module are shown in brackets are The autosampler front cover must be in place when Start and End are selected Start Change Moves the safety flap away from the needle and positions the needle approximately 15 mm above the needle seat Needle Up Up Arrow Press function key couple of times to move the needle arm up in 2 mm steps Needle Down Down Arrow Press function key couple of times to move the needle arm down in 2 mm steps The lowest position end position is used to align the needle at the correct position in the needle seat End Done Completes the procedure by moving the gripper arm to the home position and releasing the safety flap Using the Change Needle Function 1 Ensure the front cover is installed 2 Select Start Change to move the needle arm to the maintenance position 81 Troubleshooting and Test Functions Change Needle 3 Remove the front cover NOTE Do not remove the front cover until the needle arm is in its maintenance position Removing the cover while the needle arm is activated may lock up the system 4 Exchange the needle or needle seat see Needle Seat Assembly on page 111 and Needle Assembly on page 108 5 Replace the front cover 6 Select End Done to complete the procedure 82 Tro
89. ector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies Default Addresses Autosampler 28 Autosampler 28 Pump 22 RID 29 FLD 23 VWD 24 Autosampler HP 1050 18 Agilent 8453A 25 Pump HP 1050 16 DAD MWD 26 VWD HP 1050 10 Column Compartment 27 DAD AP 1050 17 CAN Interface The CAN is an intermodule communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement Remote Interface The APG remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique 232 Theory of Operation Interfaces To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize t
90. eed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could cause a possible loss of data Do not proceed beyond a caution until you have fully understood and met the indicated conditions 281 WARNING WARNING WARNING NOTE Lithium Batteries Information Lithium Batteries Information Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent type recommended by the equipment manufacturer Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandgren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 282 Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper
91. ened If the measured torque value is too high the belt needs to be loosened For this slide the motor on the holder bracket in the appropriate direction and test the tension with the Torque2 mac macro Repeat this steps until the values are in the appropriate torque range Exchange the sample transport assembly part number G1313 60009 or G1329 60009 Exchange the main board part number G1313 69520 or G1329 69520 100 Repairing the Autosampler Instructions on simple routine repair procedures as well as more extensive repairs requiring exchange of internal parts WARNING Repairing the Autosampler Simple Repairs The autosampler is designed for easy repair The most frequent repairs such as change and needle assembly change can be done from the front of the instrument with the instrument in place in the system stack These repairs are described in Simple Repairs on page 102 Exchanging Internal Parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the autosampler from the stack removing the covers and disassembling the autosampler To prevent personal injury the power cable must be removed from the instrument before opening the autosampler cover Do not connect the power cable to the autosampler while the covers are removed Safety Flap Flex Board It is strongly recommended that the exchange of the safety flap and flex board is d
92. er that maintenance needs to be scheduled 213 WARNING Figure 37 Introduction to the Autosampler Electrical Connections Electrical Connections Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Autosampler Electrical Connections F f EE iS e ia MENN woe m a Pevzsese7o MEENE aay i b ma er 4 lt J A The GPIB connector is used to connect the autosamplers with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your autosamplers The switches are preset to a default address see Table 50 on page 232 which is recognized once after power on The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1100 Series module data transfer and synchronization The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare and so on The RS 232 connector may be used to control the autosamplers from a
93. er the module into its alternate system betore you can update its firmware Press Transfer to pass comro to the alternate system After the reboot press Execute to update the modules firmware Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Records to select the ALS Use the F5 key FW Update to enter the Update section Select the a file from the PCMCIA card 13138nnnn DLB or 1329nnnn DLB and press execute When the update has finished the update press F7 key Transfer to return the module into the normal mode LED on module should stays yellow uuc 0 008 Ready Select File KELE M ey a Status Idle You need to transfer the module into its alternate system betore you can update its firmware Press Transfer to pass contro to the alternate system After the reboot press Execute to update the modules firmware 0 262 Introduction to the Control Module Screens available from the Records screen Maintenance Use the Esc key to receive Views on the F5 key Choose System from the activities pull down menu Use the F3 key Records to select the ALS Use the F4 key Maint log to view and edit the maintenance logbook uuc 0 008 Ready Message Date Injection valve seals replaced 34313 S D Luke 07 02 99 15 33 00 Needle seat pair replaced 07 02 99 15 29 04 end of logbook Use the F7 key Add to add new maintenance activities Records Ti
94. erent Autosampler Supported trays for the Autosampler G1313A Description Part Number Tray for 100 x 2 ml vials G1313 44500 Halftray for 15 x 6 ml vials G1313 44503 Halftray for 40 x 2 ml vials G1313 44502 Supported tray for the Autosampler G1329A G1389A G2260A Description Part Number Thermostattable Tray for 100 x 2 ml vials 61329 60001 Halftray for 15 x 6 ml vials for G2260A only 61313 44503 This tray is not recommended when using a thermostat Half Tray Combinations Half trays can be installed in any combination enabling both 2 ml and 6 ml vials to be used simultaneously Numbering of Vial Positions The standard 100 vial tray has vial positions 1 to 100 However when using two half trays the numbering convention is slightly different The vial positions of the right hand half tray begin at position 101 as follows Left hand 40 position tray 1 40 Left hand 15 position tray 1 15 Right hand 40 position tray 101 140 Right hand 15 position tray 101 115 33 Installing the Autosampler Sample Trays Numbering of Tray Positions Figure 12 UE T p Pe us M W it U T A bp Exe oO no oO c o a gt 5 Position 1 Position 101 Right hand half tray 34 Table 8 Table 9 Installing the Autosampler Choice of Vials and Caps Choice of Vials and Caps List of Compatible V
95. es are provided at the initial installation point Installation and onsite warranty services are available only in Agilent service travel areas and only in the country of initial purchase unless buyer pays Agilent international prices for the product and services Warranties requiring return to Agilent are not limited to the country of purchase For installation and warranty services outside of Agilent s service travel area Agilent will provide a quotation for the applicable additional services If products eligible for installation and onsite warranty services are moved from the initial installation point the warranty will remain in effect only if the customer purchases additional inspection or installation services at the new site The foregoing warranty shall not apply to defects resulting from 1 improper or inadequate maintenance adjustment calibration usage of corrosive solvent as described in the solvent information note later in his chapter or operation buyer 2 buyer supplied software hardware interfacing or consumables 276 Warranty Statement unauthorized modification or misuse 4 operation outside of the environmental and electrical specifications for the product 5 improper site preparation and maintenance or 6 customer induced contamination or leaks THE WARANTY SET FORTH IS EXCLUSIVE AND NO OTHER WARRANTY WHETHER WRITTEN OR ORAL IS EXPRESSED OR IMPLIED AGILENT SPECIFICALLY DISCLAIMS THE IMPLIED WAR
96. es other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bench Space The autosampler dimensions and weight see Table 1 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches at the rear for the circulation of air and room for electrical connections Ensure the autosampler is installed in a horizontal position The thermostatted autosampler dimensions and weight see allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 25 cm 10 inches of space on either side for the circulation of air and approximately 8 cm 3 1 inches at the rear for electrical connections Ensure the autosampler is installed in a level position If a complete Agilent 1100 Series system is to be installed on the bench make sure that the bench is designed to carry the weight of all the modules For a complete system including the thermostatted autosampler it is recommended to position the modules in two stacks see Optimizing the Stack Configuration on page 22 Make sure that in this configuration there is 25 cm 10 inches space on either side of the thermostatted autosampler for the circulation of air 15 Installing the Autosampler Site Requirements Environment Your autosampler will work within s
97. ess otherwise stated all information in this section is valid for all models The Agilent 1100 Series autosamplers and Agilent 1100 Series thermostatted autosamplers are designed for use with other modules of the Agilent 1100 Series LC system with the HP 1050 Series or with other LC systems if adequate remote control inputs and outputs are available The autosamplerss are controlled from the Agilent 1100 Series control module or from the Agilent ChemStation for LC Three sample rack sizes are available for the autosamplers The standard full size rack holds 100 x 1 8 ml vials while the two half size racks provide space for 40 x 1 8 ml vials and 15 x 6 ml vials respectively Any two half size racks trays can be installed in the autosamplers simultaneously A specially designed sample rack holding 100 x 1 8 ml vials is available for use with the thermostatted autosamplers The half size racks trays are not designed for an optimal heat transfer when they are used with a thermostatted autosampler The autosamplers transport mechanism uses an X Z Theta movement to optimize vial pick up and return Vials are picked up by the gripper arm and positioned below the sampling unit The gripper transport mechanism and sampling unit are driven by motors Movement is monitored by optical sensors and optical encoders to ensure correct operation The metering device is always flushed after injection to ensure minimum carry over The standard analytical head d
98. essage is generated e Defective or dirty position sensor e Defective motor e Sticking spindle assembly e Defective ASM board Exchange the sampling unit flex board Exchange the needle drive motor Exchange the spindle assembly or sampling unit assembly Exchange ASM board OO O O 69 Probable Causes Suggested Actions Troubleshooting and Test Functions Needle Down Failed Needle Down Failed The needle arm failed to move down into the needle seat The lower position of the needle arm is monitored by a position sensor on the sampling unit flex board The sensor detects the successful completion of the needle movement to the needle seat position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated OO O O do Needle installed incorrectly or wrong needle type too long Defective or dirty position sensor Defective motor Sticking spindle assembly Defective ASM board Ensure the correct needle type is used and installed correctly Exchange the sampling unit flex board Exchange the needle drive motor Exchange the spindle assembly or sampling unit assembly Exchange the ASM board 70 Probable Causes Suggested Actions Troubleshooting and Test Functions Missing Vial Missing Vial No vial was found in the position defined in the method or sequence When the gripper arm picks a vial out of the s
99. evice provides injection volumes from 0 1 100 ul The micro analytical head device provides injection volumes from 0 1 40 ul Two preparative head devices provide injection volumes from 0 1 900 ul One head is limited by a system pressure of 200 bars the other by a system pressure of 400 bars The six port only 5 ports are used injection valve unit is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosamplers and connects flow from the pump to the column directly During injection and analysis the valve unit directs the flow through the autosamplers which ensures that all of the sample is injected onto the column and that the metering unit and needle are always 202 Figure 30 ASM board FA WOLD J K MMMM AN MLL Power supply Vial Tray Introduction to the Autosampler Introduction to the Autosampler free from sample residue before the next sampling sequence begins Different valves for the standard the micro and the preparative autosampler are available Control of the vial temperature in the thermostatted autosampler is achieved using an additional Agilent 1100 Series module the ALS thermostat Details of this module are given in the Agilent 1100 Series thermostatted autosampler Supplemental Manual Overview of the Autosampler Transport assembly Sampling Unit y A L L VANIS VAS MMMM Analytical head Switc
100. ey 2 5 mm 15 cm long straight handle ESD wrist strap Finger caps x3 reorder gives pack of 15 Front door cooled autosampler Air channel adapter Tray for 15 x 6 ml vials x2 Union loop extension Seat extension capillary 500 ul Seat extension capillary 1500 ul Sampler Column capillary Part Number 5063 6527 5064 8240 5181 1519 5182 0714 5182 0717 5989 3890 8710 0510 8710 2391 8710 2392 8710 2394 8710 2412 9300 1408 5063 6506 G1329 40301 G1329 43200 G1313 44503 5022 2133 61313 87307 61313 87308 G2260 87300 21 Figure 1 Installing the Autosampler Optimizing the Stack Configuration Optimizing the Stack Configuration If your autosampler is part of a system you can ensure optimum performance by installing the autosampler in the stack in the position shown in Figure 1 and Figure 2 Figure 3 and Figure 4 show the configuration recommended for a thermostatted autosampler These configurations optimize the system flow path ensuring minimum delay volume Recommended Stack Configuration for an Autosampler Front View Solvent cabinet Vacuum degasser Control module Autosampler or Micro Autosampler or Preparative Autosampler hk a Lh Column compartment Detector 22 Installing the Autosampler Optimizing the Stack Configuration Figure 2 Recommended Stack Configuration for an Autosampler Rear View
101. he POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level is defined as e standard TTL levels 0 V is logic true 5 V is false e fan out is 10 e input load is 2 2 kOhm against 5 V and e outputs are open collector type inputs outputs wired or technique 233 Theory of Operation Interfaces Table 51 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start i
102. he pull down menu in the autosampler Test 89 Table 14 Troubleshooting and Test Functions Step Commands Injector Step Commands Step Action Comments Bypass Plunger Home Needle Up Vial to Seat Needle into Vial Draw Needle Up Vial to Tray Needle into Seat Mainpass Switches injection valve to the bypass position Moves the plunger to the home position Lifts the needle arm to the upper position Moves the selected vial to the seat position Lowers the needle into the vial Metering device draws the defined injection volume Lifts the needle out of the vial Returns the selected vial to the tray position Lowers the needle arm into the seat Switches the injection valve to the mainpass position Command also switches the valve to bypass if it is not already in that position Command also lifts the needle to the upper position Command also positions the vial at the seat and lifts the needle to the upper position Command also positions the vial at the seat lifts the needle and lowers the needle into vial Command can be done more than once maximum draw volume of 100u cannot be exceeded Use Plunger Home to reset the metering device Command also switches the valve to bypass if it is not already in that position Command also lifts the needle to the upper position Command also returns the vial to the tray p
103. hed off after parking the arm Before parking the gripper arm ensure there is no vial in the gripper Use the Release Gripper function to remove the vial User Interface In the ChemStation the Park Arm command is part of the ALS maintenance positions that can be selected from the Maintenance menu in the Diagnosis display In the Control Module the Park Gripper command is located in the Control display of the autosampler The commands for the ChemStation and Control Module different names for commands in the Control Module are shown in brackets are Park Arm Park Gripper moves the gripper arm to the park position Home moves the gripper arm out of the park position to the home position Using the Park Arm Function Select Park Arm Park Gripper When the arm is in the park position the autosampler is ready for shipment and can be switched off 84 Troubleshooting and Test Functions Change Gripper Change Arm Change Gripper Change Arm The change gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism User Interface The commands for the ChemStation and Control Module different names for commands in the Control Module are shown in brackets are Start Change Moves the transport assembly and gripper arm to the position required to change the gripper arm End Done Repositions the transport assembly and gripper arm to th
104. hen the metering unit has drawn the required volume of sample into the sample loop the needle is raised and the vial is replaced in the sample tray The needle is lowered into the needle seat and the injection valve switches back to the mainpass position flushing the sample onto the column Figure 34 Mainpass Position Sample Injection 206 Introduction to the Autosampler Sampling Unit Sampling Unit The sampling unit comprises three main assemblies needle drive metering device and injection valve NOTE The replacement sampling unit excludes the injection valve and metering head assemblies The sampling units for the standard the thermostatted the micro and the preparative autosamplers are different Figure 35 Autosampler Sampling Unit Needle drive Metering device Injection valve 207 Table 42 Introduction to the Autosampler Sampling Unit Needle Drive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly The upper and lower needle positions are detected by reflection sensors on the sampling unit flex board while the needle in vial position is determined by counting the motor steps from the upper needle sensor position Analytical head preparative head The analytical head is driven by the stepper motor connecte
105. hing Valve Needle Gripper Arm 203 Introduction to the Autosampler Sampling Sequence Sampling Sequence The movements of the autosamplers components during the sampling sequence are monitored continuously by the autosamplers processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence is not completed successfully an error message is generated Solvent is bypassed from the autosamplers by the injection valve during the sampling sequence The sample vial is selected by a gripper arm from a static sample rack or from external vial positions The gripper arm places the sample vial below the injection needle The required volume of sample is drawn into the sample loop by the metering device Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 2 The plunger of the metering device moves to the initialization position 3 The gripper arm moves from the home position and selects the vial At the same time the needle lifts out of the seat The gripper arm places the vial below the needle The needle lowers into the vial The metering device draws the preset sample volume The needle lifts out of the vial oro Oo A If the automated needle wash is selected see
106. ials and Caps For reliable operation vials used with the Agilent 1100 Series autosampler must not have tapered shoulders or caps that are wider than the body of the vial The vials in Table 8 Table 9 and Table 10 and caps in Table 11 Table 12 and Table 13 shown with their Part numbers have been successfully tested using a minimum of 15 000 injections with the Agilent 1100 Series autosampler Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass 2 5182 0543 5183 4492 5183 4494 write on spot Amber glass 2 5182 3376 5183 4493 5183 4495 write on spot Polypropylene 1 5182 0567 5183 4496 wide opening Polypropylene 0 3 9301 0978 wide opening Snap Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0544 5183 4504 5183 4507 Clear glass 2 5182 0546 5183 4505 5183 4508 write on spot Amber glass 2 5182 0545 5183 4506 5183 4509 write on spot 35 Table 10 Table 11 Table 12 Installing the Autosampler Choice of Vials and Caps Screw Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 5182 0714 5183 2067 5183 2070 Clear glass 5182 0715 5183 2068 5183 2071 write on spot Amber glass 5182 0716 5183 2069 5183 2072 write on spot Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE
107. ides a basic set of functions to each module Common Electronics Core processor MC68332 Core memory The core unit has 3 memory blocks 128k 16 bit PSRAM 1M 8 Flash memory 32k 8 NVRAM 24 8 serial NVRAM from the real time clock Communication Interfaces The core unit supports direct the following interfaces CAN bus GPIB RS232 Remote MIO ASIC Application Specific Integrated Circuit The application specific integrated circuit ASIC provides interfacing to external devices through drivers including GPIB CAN APG Remote It is directly connected to the four control LEDs located near the connectors on this board and the 8 bit configuration switch which is used to configure the address for the GPIB communication baud rate for RS 232 transfer and so on Also the ASIC controls and drives module specific functions and reads static status signals Leak Converter Solvent leaking from the autosampler cools down the PTC This changes the resistance of the PTC causing the leak converter to generate a leak 220 Theory of Operation Autosampler Main Board ASM signal The leak converter consists of a PTC for leak sensing and an NTC for ambient temperature compensation This configuration ensures ambient temperature changes do not affect the leak sensing circuit Fan Drive The fan speed two speeds are possible is controlled by the main processor according to the internal heat distribution inside the module
108. ill turn into 19200 baud 8 data bit with no parity Forced Cold Start Settings Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnose and repair log books which will not be erased If you use the following switch settings and power the instrument up again a forced cold start has been completed Forced Cold Start Settings Mode Select 1 2 3 4 5 6 7 8 TEST BOOT 1 1 0 0 0 0 0 1 To return to normal operation set switches back to your GPIB or RS 232 configuration settings Stay Resident Settings Firmware update procedures may require this mode in case of firmware loading errors 239 Table 60 Theory of Operation Setting the 8 bit Configuration Switch Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a detector It only uses basic functions of the operating system for example for communication Stay Resident Settings Mode Select
109. ing maintenance see Maintenance Functions on page 80 Tray Alignment Tray alignment is required after repair of internal components or after a firmware update The procedure aligns the gripper arm correctly to ensure the positioning of the gripper arm is correct for all vials see Tray Alignment on page 87 Step Commands The step functions provide the possibility to execute each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair see Step Commands on page 89 50 Troubleshooting and Test Functions Status Indicators Status Indicators Two status indicators are located on the front of the autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Figure 13 Location of Status Indicators Status indicator Power supply indicator 51 Troubleshooting and Test Functions Power Supply Indicator Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions e When the status indicator is OFF and power switch light is on the instrument is in a prerun condition and is ready to begin an analysis
110. ion of mechanical hardware electronics and instrument interfaces Autosampler Control and Electronics The ASM board controls the vial transport mechanism sampling needle metering unit and high speed injection valve These devices are controlled by a versatile electronics design based upon a 68000 family processor which also contains battery backup RAM flash ROM a real time clock and several communications options 218 Table 44 Table 45 Theory of Operation Position and Movement Sensors Position and Movement Sensors Position sensing of movement of autosampler components is done by sensors on the sample transport and sampling unit flex boards The following sensors are used Sample Transport Flex Board Sensor Type Number of Sensors _ Position Movement Sensed Reflection Sensor 6 Vial tray identification Reflection Sensor 1 Gripper initialization Reflection Sensor 3 Transport assembly Initialization Sampling Unit Flex Board Sensor Type Number of Sensors Position Movement Sensed IR light sensor 1 Metering device home reference position Reflection sensor 2 Needle end positions Hall sensor 2 Front cover in position Microswitch 2 Valve switching 219 Table 46 Theory of Operation Autosampler Main Board ASM Autosampler Main Board ASM Common Electronics A common electronics and firmware design is used for all Agilent 1100 Series LC modules This core design prov
111. kage When the seat shows indication of 10 minutes damage or blockage After approximately 30000 to 40000 30 minutes injections or when the valve performance shows indication of leakage or wear When autosampler reproducibility 30 minutes indicates seal wear Exchanging the gripper When the gripper arm is defective 10 minutes arm Time Required Notes See Needle Assembly on page 108 See Needle Seat Assembly on page 111 See Rotor Seal on page 117 See Metering Seal and Plunger on page 120 See Gripper Arm on page 123 107 Repairing the Autosampler Needle Assembly Needle Assembly Frequency Tools required Parts required When the needle is visibly damaged When the needle is blocked Ya inch wrench supplied in accessory kit 2 5 mm Hex key supplied in accessory kit Needle assembly G1313 87201 for G1313 87101 or G1313 87103 needle seat Needle assembly G1329 80001 for G1329 87101 or G1329 87103 needle seat Needle assembly 900 ul loop cap G1313 87202 for G1313 87101 needle seat Needle assembly 900 ul loop cap G2260 87101 for G2260 87101 needle seat WARNING C1 When the needle is positioned 15 mm above the needle seat remove the front cover To avoid personal injury keep fingers away from the needle area during autosampler operation Do not bend the safety flap away from its position or attempt to insert or remove a vial from the gripper
112. key 9 64 mm 15 cm long T handle Hex key 2 5 mm 15 cm long straight handle ESD wrist strap Finger caps x3 Tray for 40 x 2 ml vials Tray for 15 x 6 ml vials Capillary 0 17 mm 180 mm no PN no PN 5181 1516 5182 0714 5182 0717 no PN no PN 8710 0510 8710 2391 8710 2392 8710 2394 8710 2412 no PN 5063 6506 G1313 44502 G1313 44503 G1313 87305 Reorder gives pack of 15 177 Parts and Materials Thermostatted Autosampler Accessory Kit G1329 68705 Thermostatted Autosampler Accessory Kit G1329 68705 Table 34 G1329A Thermostatted Autosampler Accessory Kit Contents G1329 68705 Description Part Number Tubing assembly no PN Filter promo kit no PN CAN cable 1 m long 5181 1519 Screw cap vials clear 100 pk 5182 0714 Blue screw caps 100 pk 5182 0717 Label halftray no PN Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel adapter G1329 43200 Cover insulation no PN Capillary 0 17 mm 900 mm 61329 87300 Capillary heat exchanger 01090 87306 Note for 1100 Series Autosampler door upgrade no PN 178 Table 35 Parts and Materials Micro Thermostatted Autosampler Accessory Ki
113. l microvial or 1 ul from 10 ul sample in 300 ul microvial Typically lt 0 1 lt 0 05 with external needle cleaning 0 2 50 cp 100 x 2 ml vials in 1 tray 40 x 2 ml vials in 2 tray 15 x 6 ml vials in tray Agilent vials only Typically 50 s depending on draw speed and injection volume 271 Table 64 Specifications Performance Specifications Performance Specifications Agilent 1100 Series Thermostatted Micro Autosampler G1389A Type Sample capacity Settable injection volume Precision Minimum sample volume Carryover Sample viscosity range Recommended pH range Material in contact with solvent GLP features Communications Safety features Housing Specification 100 x 2 ml vials in 1 tray Microvials 100 or 300 ul with sleeves reduced cooling performance with microvials 0 01 to 8 ul with small loop capillary 0 01 to 40 ul with extended loop capillary Typically lt 0 5 RSD from 5 to 40 ul Typically lt 1 RSD from 1 to 5 ul Typically lt 3 RSD from 0 2 to 1 ul 1 ul from 5 ul sample in 100 ul microvial or 1 ul from 10 ul sample in 300 ul microvials Typically lt 0 1 without automated needle wash Typically lt 0 05 with external needle cleaning and 1 ul injection volume 0 2 5 cp 1 0 8 5 solvent with pH lt 2 3 should not contain acids which attack stainless steel Upper pH range is limited by fused silica capillaries Stainless steel sapphi
114. le 55 Table 56 Table 57 Theory of Operation Setting the 8 bit Configuration Switch Communication Settings for RS 232C Communication The communication protocol used in this instrument supports only hardware handshake CTS RTS Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory Communication Settings for RS 232C Communication Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baud rate Data Parity Bits Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Baud Rate Settings Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Data Bit Settings Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication One start bit and one stop bit are always used not selectable 238 Table 58 CAUTION Table 59 Theory of Operation Setting the 8 bit Configuration Switch Parity Settings Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity Per default the module w
115. low the lower limit the error message is generated e Defective ASM board 1 Exchange the ASM board 62 Probable Causes Suggested Actions Troubleshooting and Test Functions Fan Failed Fan Failed The cooling fan in the autosampler has failed The hall sensor on the fan shaft is used by the ASM board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 seconds the error message is generated e Fan cable disconnected e Defective fan e Defective ASM board Ensure the fan is connected correctly Exchange fan Exchange the ASM board Ooo 63 Troubleshooting and Test Functions Open Cover Open Cover The top foam has been removed The sensor on the ASM board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable Causes e The top foam was removed during operation e Foam not activating the sensor e Sensor defective Suggested Actions LI Replace the top foam 1 Exchange the ASM board 64 Probable Causes Suggested Actions Troubleshooting and Test Functions Restart Without Cover Restart Without Cover The autosampler was restarted with the top cover and foam open The sensor on the ASM board detects when the top foam is in place If the autosampler is restarted with the foam removed the autosampler switches off within 30 s and the error message is generated
116. m wrench for GPIB connector screws ASM board 61313 69520 for G1373A ASM board 61329 69500 for G1329A G1389A G2260A CAUTION NOTE m_a ees Before beginning this procedure L Remove the top cover and foam page 128 LI Remove the transport assembly page 132 The ASM board is sensitive to electrostatic discharge Always use the ESD strap see Using the ESD Strap on page 105 when handling electronic boards This procedure requires reloading the autosampler firmware reprogramming of the instrument serial number and realignment of the gripper 1 Remove the connector screws from the GPIB and RS232 connectors 148 Repairing the Autosampler ASM Board 2 Disconnect all connectors on ASM board Jf WMUMLLW Y WLLL WML OE 4 3 Slide ASM board out of the autosampler 4 Install new board Ensure ribbon cables are positioned in the slot in the board 5 Reconnect the connectors to the board a7 Cte WH J GPIB Screws On completion of this procedure On the new board check the switch setting of address switch S1 see Table 53 on page 236 or Table 54 on page 237 Note An incorrect switch setting e g TEST BOOT may cause the autosampler to turn in a basic mode yellow or red flashing status light In such a case turn off the pump re set the address switches and turn on the pump again
117. mal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extreme mobile phase conditions PEEK Seal for preparative injection valve only The preparative injection valve has a sealing material made of PEEK This material has high chemical resistance and versatility It is suitable for application using mobile phases within a pH between 1 and 14 Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible with PEEK 48 Troubleshooting and Test Functions The modules built in troubleshooting and test functions Troubleshooting and Test Functions Status Indicators The autosamplers are provided with two status indicators which indicate the operational state prerun run and error states of the instrument The status indicators provide a quick visual check of the operation of the autosampler see Status Indicators on page 51 Error Messages In the event of an electronic mechanical or hydraulic failure the instrument generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see Error Messages on page 53 Maintenance Functions The maintenance functions position the needle arm gripper assembly and metering device for easy access when do
118. me MEAT idie Ready Needle seat pair replaced e Injec Injection valve seals replaced Neec alice Add 263 Introduction to the Control Module Diagnostics and Tests Diagnostics and Tests Tests screen Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Tests to select the ALS Several tests are available to test the Agilent 1100 autosampler Additional test are listed in the function box Press the m key to open the context sensitive menu Menu item 3 Schematics will popup the ALS and ALS thermostat diagram Tests ies onoke Ready Function ERAO uuc 0 008 Ready 2Temp On Off i 3Schematics Sampler Schematics Thermo 264 Introduction to the Control Module Diagnostics and Tests uuc 0 008 265 Introduction to the Control Module Diagnostics and Tests Tray and Transport Use the F1 key Align to align the autosampler tray or transport Time HORT Idle Ready Alignment 266 Introduction to the Control Module Diagnostics and Tests Change Needle Use the F2 key Change to start the maintenance procedures for changing Piston and Arm the autosampler needle piston and gripper arm uuc 0 008 Change Needle Change Piston Change Arm Change Signals Use the F8 key Signals to display the signals available from the ALS thermostat Press F6 key Setup Here you can select which signals are show
119. mit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The autosamplers provides the following EMF counters Injection valve counter This counter display the total number of switches of the injection valve since the last reset of the counter Needle Movements Counter This counter displays the total number of movements of the needle into the seat since the last reset of the counter Using the EMF Counters The user setable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of autosamplers components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument 212 Introduction to the Autosampler Early Maintenance Feedback EMF Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary make note of the values displayed by the injection valve and needle movements counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a remind
120. n Tests Time 0 OOF Ready Aux 1 Heat Sink Left E 1 501 Aux 2 ERMScukn Cas 6480 00 267 Introduction to the Control Module Diagnostics and Tests 268 Specifications Performance specifications of the Agilent 1100 Series Standard Micro and Preparative Autosamplers Table 62 Specifications Performance Specifications Performance Specifications Performance Specifications Agilent 1100 Series Autosampler G1313A and Thermostatted Autosampler G1329A Valid when standard 100 pl metering head installed Type Specification GLP features Early maintenance feedback EMF electronic records of maintenance and errors Communications Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Safety features Leak detection and safe leak handling low voltages in Injection range Replicate injections Precision Minimum sample volume Carryover Sample viscosity range Replicate injections per vial Sample capacity Injection cycle time maintenance areas error detection and display 0 1 100 ul in 0 1 ul increments Up to 1500 ul with multiple draw hardware modification required 1 99 from one vial Typically lt 0 5 RSD of peak areas from 5 100 ul Typically lt 1 RSD of peak areas from 1 5 ul 1 ul from 5 ul sample in 100 ul microvial or 1 ul from 10 ul sample in 300 ul microvial
121. n the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated e Defective injection valve e Defective ASM board 1 Exchange the injection valve 1 Exchange the ASM board 67 Probable Causes Suggested Actions Troubleshooting and Test Functions Valve to Mainpass Failed Valve to Mainpass Failed The injection valve failed to switch to the mainpass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated e Defective injection valve e Defective ASM board 1 Exchange the injection valve 1 Exchange the ASM board 68 Probable Causes Suggested Actions Troubleshooting and Test Functions Needle Up Failed Needle Up Failed The needle arm failed to move successfully from the seat or out of the vial to the upper position The upper position of the needle arm is monitored by a position sensor on the sampling unit flex board The sensor detects the successful completion of the needle movement to the upper position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error m
122. n Cover 127 Repairing the Autosampler Top Cover and Foam Top Cover and Foam Frequency Tools required Parts required When accessing internal parts If interface board installed Flat head screwdriver Foam kit G1313 68702 CAUTION This procedure requires removal of the MIO interface board The board is sensitive to electrostatic discharge Always use the ESD strap when handling electronic boards 1 Switch off the autosampler at the main poweri 2 If installed remove the ECB board switch Remove the power cable on the top cover 3 Move the safety lever to the left Lift the clips 4 Lift the cover away safety lever 128 Repairing the Autosampler Top Cover and Foam 5 Unscrew the three screws on the top plate Remove the top plate 6 Carefully remove the top foam 129 Repairing the Autosampler Installing the Top Cover and Foam Installing the Top Cover and Foam Frequency When accessing internal parts Tools required If interface board installed Flat head screwdriver Parts required None 1 Install the top foam Press the foam firmly 2 Engage the slot on the top plate onto the side into place plate 3 Fix the top plate in place with the three 4 Engage the top cover into the bottom cover screws 130 Repairing the Autosampler Installing the Top Cover and Foam
123. n list the timetable for the ALS Press F7 key Insert to add entries or F6 key Delete to remove entries Timetable EMA FEI 0DE owi o cos Ready Time Module Setting 0 00 Autosampler External Contact 1 Open end of module timetable Press the F7 key Insert to add timetable events Use the F6 key Done to view the entered lines of the timetable Setting External Contact 1 8 Contact State LEER fields change with setting selected lt gt 252 Introduction to the Control Module Screens available from the Analysis screen Use the m key for the context sensitive menu It gives you additional tools for the timetable le BECCEee ORYE ONDE Ommu 0 OOmGIE Ready Time Module Setting Print Timetabi Settings Injection With the F3 key Inj Program you can change the settings for the analog Program outputs SSAA OREI Offed oiua 0 O008GI Ready Line Function Parameters cS press to edit line gt 1 DRAW 2 0 ul from vial 1 2 DRAW 2 0 pl from vial 2 Press the F7 key Insert to add program events Use the F6 key Done to view the entered lines of the timetable Line Function 1 EA 253 Introduction to the Control Module Screens available from the Analysis screen Use the m key for the context sensitive menu It gives you additional tools for the timetable The F8 key Validate will check the injector program for errors a Insert Delete
124. ng Home Failed 73 Motor Temperature 74 Initialization with Vial 75 Safety Flap Missing 76 Vialin Gripper 77 Missing Wash Vial 78 Invalid Vial Position 79 Maintenance Functions 80 Change Needle 81 Change Piston 83 Contents Park Arm Park Gripper 84 Change Gripper Change Arm 85 Tray Alignment 87 Step Commands 89 Troubleshooting Guide for the Sample Transport Assembly 94 Intermittent lock ups with or without vial in the gripper fingers 95 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the via 97 Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm 99 Repairing the Autosampler Instructions on simple routine repair procedures as well as more extensive repairs requiring exchange of internal parts 101 Updating the Firmware 103 Cleaning the Autosampler 104 Using the ESD Strap 105 Overview of Main Repair Procedures 106 Simple Repairs 107 Needle Assembly 108 Needle Seat Assembly 111 Stator Face 114 Contents Rotor Seal 117 Metering Seal and Plunger 120 Gripper Arm 123 Interface Board 125 Exchanging Internal Parts 126 Assembling the Main Cover 127 Top Cover and Foam 128 Installing the Top Cover and Foam 130 Transport Assembly 132 Sampling Unit 134 Injection Valve Assembly 140 Metering Drive Motor and Belt 142 Needle Drive Motor and Belt 144 Fan 146 ASM Board 148 SUD Board 1
125. nge Piston on page 83 122 Repairing the Autosampler Gripper Arm Gripper Arm Frequency Defective gripper arm Tools required Straightened paper clip Parts required Gripper assembly 61313 60010 I Before beginning this procedure 1 Select Start in the maintenance function ChangeGripper see Change Gripper Change Arm on page 85 Turn off the power to the autosampler Remove the front cover 1 Identify the slit below the gripper motor and the gripper arm release button 2 Rotate the arm approximately 2 5cm 1 inch to the left and insert the straightened paper clip into the slit 3 Rotate the gripper arm slowly from left to right and apply a gentle pressure to the paper clip The clip will engage on an internal catch and the rotation of the arm will be blocked 123 4 Hold the paper clip in place press the gripper release button and rotate the gripper arm to the right 5 The gripper arm will come off 6 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left On completion of this procedure 1 Install the front cover A La 5 1 Turn the power to the autosampler on 124 Repairing the Autosampler Interface Board Interface Board Frequency Tools required Pa
126. njection cycle for example by start key on any module Receiver is the autosampler 234 Table 52 Theory of Operation Interfaces RS 232C The RS 232C connector is used to control the instrument from a computer through RS 232C connection using the appropriate software This connector can be activated by the configuration switch module next to the GPIB connector The RS 232C is designed as DCE Data Communication Equipment with a 9 pin male SUB D type connector The pins are defined as follows RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI RS 232 Cable Instrument PC 1 1 DCD 2 v 2 RX 3 3 TX 4 4 DTR 5 Y 5 GND 6 6 DSR 7 v 7 RTS 8 N 8 CTS 9 9 RI DB9 DB9 DB9 Female Female Male 235 Figure 44 factory setting is shown for autosampler Table 53 Theory of Operation Setting the 8 bit Configuration Switch Setting the 8 bit Configuration Switch The 8 bit configuration switch is located next to the GPIB connector Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures 8 bit Configuration Switch 8 bit Configuration Switch Mode Select 1 2 3 4 5 6 7 8 GPIB 0 0 GPIB Address RS 232C 0 1 Baudrate Data
127. nnected Brown green 14 Not connected White yellow 156 Not connected 198 Parts and Materials RS 232 Cable Kit RS 232 Cable Kit This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1100 module to PC RS 232 Cable Kit 34398A Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 3 TX DTR 4 4 DTR GND 5 y 5 GND DSR 6 6 DSR RIS 7 lt 7 RTS cTs 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male 199 Parts and Materials LAN Cables LAN Cables Recommended Cables For point to point connection not using a network hub use a twisted pair cross over LAN cable P N 5183 4649 10 feet long For standard network connections using a hub use category 5 UTP cables P N G1530 61480 8 m long 200 Introduction to the Autosampler An introduction to the standard micro and preparative autosamplers and thermostatted autosamplers Introduction to the Autosampler Introduction to the Autosampler Introduction to the Autosampler Five models of Agilent 1100 Series autosamplers are available within this introduction they will be referred to as the standard the micro the preparative autosamplers and the thermostatted autosamplers Unl
128. nto the needle seat to inject sample the safety flap locks into position Next and the gripper checks the safety flap by trying to move the safety flap away from the needle If the gripper is able to move beyond the safety flap position safety flap not in position the error message is generated e Safety flap missing or broken 41 Exchange the safety flap 76 Probable Causes Suggested Actions Troubleshooting and Test Functions Vial in Gripper Vial in Gripper The gripper arm attempted to move with a vial still in the gripper During specific stages of the sampling sequence no vial should be held by the gripper The autosampler checks if a sample vial is stuck in the gripper by closing and opening the gripper fingers while monitoring the motor encoder If the gripper fingers are unable to close the error message is generated e Vial still in gripper 1 Remove the vial using the Release Vial function in the user interface Reinitialize the autosampler 77 Probable Causes Suggested Actions Troubleshooting and Test Functions Missing Wash Vial Missing Wash Vial The wash vial programmed in the method was not found When the gripper arm picks a vial out of the sample tray the processor monitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the pr
129. ocessor encoder position causing the error message to be generated e No wash vial in the position defined in the method 1 Install the wash vial in the correct position or edit the method accordingly 78 Probable Causes Suggested Actions Troubleshooting and Test Functions Invalid Vial Position Invalid Vial Position The vial position defined in the method or sequence does not exist The reflection sensors on the transport assembly flex board are used to check automatically which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated e Incorrect tray or trays installed e Incorrect vial positions defined in the method or sequence e Tray recognition defective dirty sample tray or defective transport assembly flex board 1 Install the correct trays or edit the method or sequence accordingly 41 Ensure the coding surfaces of the sample tray are clean located at the rear of the sample tray 1 Exchange the transport assembly 79 Troubleshooting and Test Functions Maintenance Functions Maintenance Functions Certain maintenance procedures require the needle arm metering device and gripper assembly to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the ChemStation the ALS maintenance
130. one by Agilent trained service personnel Transport Assembly Parts The adjustment of the motors and the tension on the drive belts are important for correct operation of the transport assembly see Transport Assembly on page 132 It is strongly recommended that exchange of drive belts and the gripper assembly is done by Agilent trained service personnel There are no other field replaceable parts in the transport assembly If any other component is defective flex board spindles plastic parts the complete unit must be exchanged 102 Repairing the Autosampler Updating the Firmware Updating the Firmware The Agilent 1100 Series LC modules are fitted with FLASH EPROMS These EPROMS enable you to update the instrument firmware from the ChemStation PCMCIA card or through the RS232 interface The firmware update procedure is described in the on line user information 103 WARNING Repairing the Autosampler Cleaning the Autosampler Cleaning the Autosampler The autosampler covers should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth that liquid can drip into the autosampler Do not let liquid drip into the autosampler It could cause a shock hazard or damage to the autosampler 104 Repairing the Autosampler Using the ESD Strap Using the ESD Strap CAUTION Electronic
131. or balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable Causes e Not ready condition in one of the instruments connected to the remote line e Defective remote cable e Defective components in the instrument showing the not ready condition Suggested Actions 1 Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis 1 Exchange the remote cable 1 Check the instrument for defects refer to the instrument s reference documentation 56 Probable Causes Suggested Actions Troubleshooting and Test Functions Sychronization Lost Sychronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated e CAN cable disconnected e Defective CAN cable e Defective main board in another module 4 Ensure all the CAN cables are connected correctly 1 Switch off the system Restart the system and determine which module or modules are not recognized by the system d Ensure all CAN cables are installed correctly 57 Troubleshooting and Test Functions L
132. or engage in bolts alternately two turns at a time until the the holes in the stator head stator head is secure 115 Repairing the Autosampler Stator Face 6 Reconnect the pump capillaries to the valve 7 Slide the waste tube into the waste holder in ports see Hydraulic Connections on the leak tray page 31 On completion of this procedure 1 Install the front cover 116 Repairing the Autosampler Rotor Seal Rotor Seal Frequency Tools required Parts required Poor injection volume reproducibility Leaking injection valve 1 4 inch wrench supplied in accessory kit Hex key 9 64 inch supplied in accessory kit 0 1853 Vespel for G1373A and G1329A 0 1849 Tefzel for G7313A and G1329A 0 2088 Vespel for G1389A 1 1268 PEEK for G2260A Rotor seal 0 Rotor seal 0 Rotor seal 0 Rotor seal 0 Ss ae Se Ss ooo OG CAUTION oa e a a eee Before beginning this procedure 1 Remove all capillary fittings from the _J Remove the front cover The stator face is held in place by the stator head When removing the stator head ensure the stator face does not fall out of the valve injection valve ports 117 Repairing the Autosampler Rotor Seal 2 Loosen each fixing bolt two turns at a time 3 Remove the stator head stator face and Remove the bolts from the he
133. osition 90 Table 14 Table 15 Troubleshooting and Test Functions Step Commands Injector Step Commands continued Step Action Comments Needle Up Mainpass Lifts the position needle arm to the upper Command available from Control and Switches the injection Module only valve to the mainpass position Troubleshooting If the autosampler is unable to perform a specific step due to a hardware failure an error message is generated You can use the injector steps to do the injection sequence while observing how the instrument responds Table 15 summarizes the injector steps and lists the associated error messages and probable causes of step failures Step Failures Step Function Bypass Plunger Home Needle Up Vial to Seat Probable Failure Modes Valve Valve Defec Defec Defec Need Defec already in bypass not connected ive injection valve ive or dirty sensor on the sampling unit flex board ive metering drive motor e already in the upper position ive or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defec ive needle drive motor No vial in selected position Vial a Defec ready in seat position ive transport assembly motors Sticking transport assembly Defec ive gripper assembly Gripper not aligned see page 87 91 Table 15 Troubleshooting and Test Functions Step Commands
134. parameters Choose menu item 1 Setup limits to select the number of injection valve cycles or number of needle movements into the needle seat at which you want to receive a warning Records fis o oE Ready G1329A Autosampler Serial DE12345678 D SIELA PUrOSSmMpaT eee Valve Cycles 34313 Limit OFF Needle into Seat 84875 Limit If a set limit has been exceeded a message box will pop up This will not stop a sequence or run the information is intended only for planning of maintenance activities If you press Reset the limits will be removed Ignore will continue to keep the EMF flag set Records u o OOF Ready Valve cycles limit exceeded l The number of valve cycles defined for the autosampler has exceeded its defined limit Module 261 Firmware Update Introduction to the Control Module Screens available from the Records screen Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Records to select the ALS Use the F5 key FW Update to enter the Update section If you want to update the resident firmware together with specific main firmware revisions select the a file from the PCMCIA card RESnnnn DLB and press execute If you want to update the main firmware press F7 key Transfer to turn the module into the resident mode LED on module should blink yellow ace 0 008 Ready Select File TSF Eee aaa Status Idle You need to transf
135. pecifications at ambient temperatures and relative humidity as described in Table 1 CAUTION Do not store ship or use your autosampler under conditions where temperature fluctuations may cause condensation within the autosampler Condensation will damage the system electronics If your autosampler was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation Table 1 Physical Specifications Autosampler G1313A G1329A G1389A G2260A Type Specification Comments Weight 14 2 kg 31 3 Ibs Dimensions 200 x 345 x 435 mm height x width x depth 8 x 13 5 x 17 inches Line voltage 100 120 or 220 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption apparent power 300 VA Maximum Power consumption active power 200 W Maximum Ambient operating temperature 4 55 C 41 131 F see WARNING on page 16 Ambient non operating temperature 40 to 70 C 4 to 158 F Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the autosampler Safety standards IEC CSA UL Installation Category Il Pollution Degree 2 WARNING Using the autosampler at environmental temperatures higher than 50 C 122 F may cause the rear panel to become hot 16 CAUTION CAUTION Installing the
136. pipe to the coupler Repairing the Autosampler Power Supply xx On completion of this procedure Install the ASM board page 148 Install the fan page 146 Install the sampling unit page 134 Install the transport assembly page 132 Install the top cover page 128 Oococo Verify the transport assembly alignment page 99 156 Repairing the Autosampler Leak Sensor Leak Sensor Frequency When defective Tools required None Parts required Leak sensor 5061 3356 s Before beginning this procedure Li Remove the top cover page 128 L Remove the transport assembly page 132 Li Remove the sampling unit page 134 sensor 1 Disconnect the leak sensor from the SUD board labelled leak sensor Remove the 2 Feed the cable of the new sensor through the 3 Connect the connector to the connector labelled leak sensor base of the sampling unit to the SUD board jf AA tl B 157 Repairing the Autosampler Leak Sensor lauu On completion of this procedure d Install the sampling unit page 134 LI Install the transport assembly page 132 LI Install the top cover page 128 Li Verify the transport assembly alignment page 99 158 Parts and Materials Detailed illustrations and lists for identification of parts and materials P
137. plunger 5064 8293 for 40 pl analytical head Metering plunger 5062 8587 for 900 pl analytical head only if scratched or contaminated Before beginning this procedure 1 Remove the two capillaries from the metering head assembly 1 Select Start in the maintenance function Change piston see Change Piston on page 83 J Remove the front cover 120 Repairing the Autosampler Metering Seal and Plunger 2 Remove the two fixing bolts and pull the head assembly away from the sampler 3 Remove the two fixing bolts from the base of the metering head assembly 5 Use a small screwdriver to carefully remove the seal Clean the chamber with lint free cloth Ensure all particular matter is removed 6 Install the new seal Press the seal firmly into position 121 Repairing the Autosampler Metering Seal and Plunger 8 Carefully insert the plunger into the base 9 Press the plunger into the seal Reassemble the metering head assembly 10 Install the fixing bolts Tighten the bolts 11 Install the metering head assembly in the securely autosampler Ensure the large hole in the metering head is facing downwards 12 Reinstall the capillaries On completion of this procedure 1 Install the front cover J Select End in the maintenance function Change piston see Cha
138. press Goto Vial Repeat steps 4 to 8 to align the gripper at position 95 Press button Average F8 to balance the alignment Press button Done F6 to store the alignment permanently in non volatile memory and to reset the module To check the result go back to the alignment dialog box move to vial position 15 and 95 to see if the alignment is acceptable To leave the alignment screen without changes use the Esc key The result can be a compromise e g if the X position at 15 and 95 are off to the same side then it is OK However if at both positions the correction still should be in one direction or if the failure in one position is larger than the other you must restart the alignment procedure with step 3 The same goes for the Theta correction 88 Troubleshooting and Test Functions Step Commands Step Commands Each movement of the sampling sequence can be done under manual control This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands which move the autosampler components to predefined positions enabling the specific step to be done In the ChemStation the step commands can be selected from the Test Selection Box in the Diagnosis display In the Control Module the step commands can be accessed from t
139. r Main Board ASM 220 Firmware Description 226 Optional Interface Boards 228 Interfaces 231 Setting the 8 bit Configuration Switch 236 The Main Power Supply Assembly 241 Introduction to the Control Module Introduction to the screens available for operation of the Ag tlent 1100 Series autosamplers with the control module 245 Screens available from the Analysis screen 247 Screens available from the System screen 258 Screens available from the Records screen 260 Diagnostics and Tests 264 10 10 Contents Specifications Performance specifications of the Agilent 1100 Series Stan dard Micro and Preparative Autosamplers 269 Performance Specifications 270 Legal Safety and Warranty Information Warranty Statement 276 Safety Information 279 Lithium Batteries Information 282 Radio Interference 283 Sound Emission 283 Solvent Information 284 Agilent Technologies on Internet 285 11 Contents 12 Installing the Autosampler Site requirements and installation of the autosampler and thermostatted autosampler WARNING WARNING WARNING Installing the Autosampler Site Requirements A suitable site environment is important to ensure optimal performance of the autosampler Power Consideration The autosampler power supply has wide ranging capability Consequently there is no voltage selector in the rear of the autosampler There are also no externally accessible fuses
140. r the customer s failure to comply with these requirements General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement Do not use repaired fuses and avoid to short circuit fuseholders Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage is forbidden 279 WARNING Table 67 Safety Information Disconnect the
141. rative autosampler When using large injection volumes setting the eject speed to a higher value speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the plunger returns to the home position An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens 46 Optimizing Performance Precise Injection Volume the time required to run the injector program When using viscous samples a high eject speed should be avoided 47 NOTE Optimizing Performance Choice of Rotor Seal Choice of Rotor Seal Vespel Seal for standard and micro valves only The standard seal has sealing material made of Vespel Vespel is suitable for applications using mobile phases within the pH range of 2 3 to 9 5 which is suitable for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime Tefzel Seal for standard valve only For mobile phases with pH below 2 3 or above 9 5 or for conditions where the lifetime of the Vespel seal is drastically reduced a seal made of Tefzel is available see Injection Valve Assembly on page 168 Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under nor
142. rder gives pack of 15 19 Installing the Autosampler Unpacking the Autosampler Table 4 G1389A Micro Thermostatted Autosampler Accessory Kit Contents G1329 68715 Description Part Number Tubing assembly 5063 6527 CAN cable 1m long 5181 1519 Screw cap vials clear 100 pk 5182 0714 Blue screw caps 100 pk 5182 0717 Label halftray 5989 3890 Vial instruction sheet no PN Fitting 79814 22406 Hex Key 8710 0060 Wrench 4 mm both ends 8710 1534 Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 Finger caps x3 9063 6506 Torque adapter 61315 45003 Front door cooled autosampler 61329 40301 Air channel adapter 61329 43200 Cover insulation no PN Extended loop capillary 0 25 mm 180 mm 61329 87302 Fused silica capillary 0 050 mm 500 mm 61375 87304 Reorder gives pack of 15 20 Table 5 Installing the Autosampler Unpacking the Autosampler G2260A Preparative Autosampler Accessory Kit Contents G2260 68705 Description Part Number Description Tubing assembly Filter promo kit CAN cable 1m long Screw cap vials clear 100 pk Blue screw caps 100 pk Label halftray Wrenches 1 4 5 16 inch Rheotool socket wrench 1 4 inch Hex key 4 mm 15 cm long T handle Hex key 9 64 mm 15 cm long T handle Hex k
143. re PTFE PEEK fused silica Vespel Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display All material recyclable 272 Table 65 Specifications Performance Specifications Performance Specifications Agilent 1100 Series Preparative Autosampler G2260A Type Pressure GLP features Communications Safety features Injection range Replicate injections Precision Minimum sample volume Sample viscosity range Sample capacity Injection cycle time Specification Operating range 0 40 MPa 0 400 bar 0 5800psi Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN GPIB IEEE 448 RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 900 ul in 0 1 ul increments recommended 1 ul increments Up to 1800 ul with multiple draw hardware modification required Up to 5000 ul with multiple draw hardware modification required 1 99 from one vial Typically lt 0 5 RSD of peak areas from 5 2000 ul Typically lt 1
144. red rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE red rubber 5181 1217 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber 5182 0550 Blue polypropylene Clear PTFE red rubber 5182 3458 Green polypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Clear PTFE red rubber 5182 3459 36 Table 13 Installing the Autosampler Choice of Vials and Caps Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red polypropylene Clear PTFE silicone 5182 0722 37 Installing the Autosampler Transporting the Autosampler Transporting the Autosampler When moving the autosampler around the laboratory no special precautions are needed However if the autosampler needs to be shipped to another location via carrier ensure The transport assembly is parked see Park Arm Park Gripper on page 84 The vial tray is secured If the autosampler is to be shipped to another location the transport assembly must be moved to the park position to prevent mechanical damage should the shipping container b
145. ries 1 Intermittent lock ups with or without vial in the gripper fingers with error messages 4 motor overtemp 0 or 1 or 2 or 3 d movement failed 0 or 1 or 2 or 3 1 missing vial Many times the sampler is being used very heavily 2 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial with error messages 1 motor overtemp 0 or 2 1 movement failed 0 or 2 3 Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm with error messages 1 motor overtemp 0 or 2 or 3 E movement failed 0 or 2 or 3 1 missing vial Motor 0 X 1 Z 2 Theta 3 Gripper 94 NOTE Step 1 Step 2 Step 3 Step 4 Step5 Troubleshooting and Test Functions Intermittent lock ups with or without vial in the gripper fingers Intermittent lock ups with or without vial in the gripper fingers With error messages J motor overtemp 0 or 1 or 2 or 3 4 movement failed 0 or 1 or 2 or 3 1 missing vial When a motor over temperature message has occurred the sampler must be turned off for about 10 minutes to allow the motor to cool down Check the firmware and update to the latest revision if necessary Since firmware revision A 03 61 resident A03 60 most movement failed 6 motor over temp initialization failed X axis errors are solved Check the vials and the caps
146. rts required At installation or when defective Flat head screwdriver Interface board see Optional Interface Boards on page 228 CAUTION Figure 16 The interface board is sensitive to electrostatic discharge Always use the ESD strap when handling electronic boards Switch off the autosampler at the main power switch Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the autosampler Install the interface board Secure the screws a AeA V N Reconnect the cables to the board connectors Exchanging the Interface Board 125 WARNING WARNING WARNING NOTE CAUTION Repairing the Autosampler Exchanging Internal Parts Exchanging Internal Parts The following procedures require opening the main cover of the autosampler Always ensure the autosampler is disconnected from the line power when the main cover is removed The security lever at the power input socket prevents the autosampler cover from being taken off when line power is still connected The power supply still uses some power even if the power switch on the front panel is turned off To disconnect the autosampler from line power unplug the power cord When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the ma
147. s drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated e Mechanical obstruction e High friction in the transport assembly e Motor belt tension too high e Defective motor e Defective transport assembly flex board 1 Switch off the autosampler at the power switch Wait at least 10 minutes before switching on again 41 Ensure unobstructed movement of the transport assembly 1 Exchange the transport assembly 74 Probable Causes Suggested Actions Troubleshooting and Test Functions Initialization with Vial Initialization with Vial The autosampler attempted to initialize with a vial still in the gripper During initialization the autosampler checks correct operation of the gripper by closing and opening the gripper fingers while monitoring the motor encoder If a vial is still in the gripper when initialization is started the gripper fingers cannot close causing the error message to be generated e Vial still in gripper 1 Remove the vial using the Release Vial function in the user interface Reinitialize the autosampler 75 Probable Causes Suggested Actions Troubleshooting and Test Functions Safety Flap Missing Safety Flap Missing The safety flap was not detected Before the needle moves down i
148. s to be performed in the correct order and without skipping parts to ensure a proper working Autosampler User Interface In the Control Module the Align Tray function is located in the Control display of the autosampler Additional information can be found in the on line information systems The correct procedure for the Control Module are 1 Set alignment to factory default e Go to Align Tray and press button Default e Go to Align Transport and press button Default e Wait while the Autosampler performs a reset and go back to 87 Troubleshooting and Test Functions Tray Alignment Align Transport 2 Put capped vials into positions 15 and 95 of the 100 vial tray 3 Move the gripper arm to position of vial 15 Use the Enter key to hit Goto Vial Use the Arm down key F2 to move the fingers as close as possible to the top of the vial without the gripper fingers touching the vial 5 Use the Up and Down arrow keys for Theta correction rotational movement 6 Use the Left and Right arrow keys for X position correction horizontal 10 11 12 13 NOTE NOTE movement Open the gripper F4 and move it further down for about 5 mm in such a way that vial cap and rubber of gripper fingers have the same height Visually re evaluate if the vial is in the center of the gripper fingers and correct X and Theta position accordingly Press the Enter key to hit Next Vial enter vial 95 and
149. scosity of the sample allows it the draw and eject speed of the injection system has to be increased For the last injection of the sequence with overlapped injections it has to be considered that for this run the injection valve is not switched as for the previous runs and consequently the injector delay volume is not bypassed This means the retention times are prolonged for the last run Especially at 44 Optimizing Performance Fast Injection Cycle and Low Delay Volume low flow rates this can lead to retention time changes which are to big for the actual calibration table To overcome this it is recommended to add an additional blank injection as last injection to the sequence General Recommendations for Fast Injection Cycle Times As described in this section the first step to provide short cycle times are optimizing the chromatographic conditions If this is done the autosampler parameter should be set to e Overlapped injection mode e Increase of draw and eject speed for large injection volumes e Add at last run a blank if overlapped injection is used To reduce the injection time the detector balance has to be set to OFF 45 Optimizing Performance Precise Injection Volume Precise Injection Volume Injection Volumes Less Than 2 pl When the injection valve switches to the BYPASS position the mobile phase in the sample loop is depressurized When the syringe begins drawing sample the mobile phase is
150. se into the autosampler a T i Uo N uS 2 5 Slide the sampling unit and tray base forwards Ensure the sampling unit connector is seated correctly 6 Install the pump and column thermostat capillaries ports 1 and 6 in the injection valve Connector 138 Repairing the Autosampler Sampling Unit 7 Install the waste tube in the holder in the leak tray On completion of this procedure 1 Install the top cover page 128 1 Install the vial tray Ll Verify the transport assembly alignment page 99 1 Install the transport assembly page 132 139 Repairing the Autosampler Injection Valve Assembly Injection Valve Assembly Frequency When defective Pozidrive No 1 screwdriver 1 4 inch wrench Tools required Parts required Injection valve 0101 0921 for G1313A G1329A Injection valve 0101 1050 for G1389A Injection valve 0101 1267 for G2260A _ ae Before beginning this procedure Li Remove all capillaries from the injection valve page 31 1 Remove the top cover page 128 m Remove the transport assembly page 132 Li Remove the sampling unit page 134 1 Disconnect the valve cable connector from the SUD board labelled
151. sion with the Torque2 mac macro Repeat this steps until the values are in the appropriate torque range Reset the sampler alignments to default value Reset tray alignment and transport alignment is possible with the Control Module and the Chemstation To reset the transport alignment with the Chemstation enter following command in the command line Print sendmodule lals tray alig 0 00 0 00 Exchange the sample transport assembly part number G1313 60009 or G1329 60009 Exchange the main board part number G1313 69520 or G1329 69520 98 NOTE Step 1 Step 2 Step 3 NOTE Troubleshooting and Test Functions Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm With Error messages LJ motor overtemp 0 or 2 or 3 LJ movement failed 0 or 2 or 3 When a motor over temperature message has occurred the sampler must be turned off for about 10 minutes to allow the motor to cool down Check the firmware and update to the latest revision if necessary Since revision A 03 61 resident A03 60 most of following movement failed motor over temp and initialization failed X axis errors are solved Reset the sampler alignment to default value Reset tray alignment and transport alignment is possible with the Control Module
152. t G1329 68715 Micro Thermostatted Autosampler Accessory Kit G1329 68715 G1389A Micro Thermostatted Autosampler Accessory Kit Contents G1329 68715 Description Tubing assembly CAN cable 1 m long Screw cap vials clear 100 pk Blue screw caps 100 pk Label halftray Fitting Hex Key Wrench 4 mm both ends Wrenches 1 4 5 16 inch Rheotool socket wrench 1 4 inch Hex key 4 mm 15 cm long T handle Hex key 9 64 mm 15 cm long T handle Hex key 2 5 mm 15 cm long straight handle ESD wrist strap Finger caps x3 reorder gives pack of 15 Torque adapter Front door cooled autosampler Air channel adapter Extended loop capillary 0 25 mm 180 mm Fused silica capillary 0 050 mm 500 mm Part Number no PN 5181 1519 5182 0714 5182 0717 no PN 5061 3303 8710 0060 8710 1534 8710 0510 8710 2391 8710 2392 8710 2394 8710 2412 no PN 5063 6506 G1315 45003 no PN G1329 43200 G1329 87302 G1375 87304 179 Parts and Materials Preparative ALS Accessory Kit G2260 68705 Preparative ALS Accessory Kit G2260 68705 Table 36 G2260A Preparative Autosampler Accessory Kit Contents G2260 68705 Description Part Number Tubing assembly no PN Filter promo kit no PN CAN cable 1 m long 5181 1519 Screw cap vials clear 100 pk 5182 0714 Blue screw caps 100 pk 5182 0717 Label halftray no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392
153. terial handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used The electronics of the autosampler will not allow operation when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the autosampler Always operate the autosampler with the top foam and top covers in place Internal components may be sensitive to electrostatic discharge ESD Always use an ESD strap when handling internal components see Using the ESD Strap on page 105 The procedures in this section describe how to exchange defective internal parts You must remove the autosampler from the stack in order to open the main cover 126 Repairing the Autosampler Assembling the Main Cover Assembling the Main Cover Tools required Parts required None G1313 68703 Cover kit for G1373A G1329 68703 Cover kit for G1329A G1389A G2260A G1329 68706 Cabinet upgrade kit for G1313A G1329A 5042 1381 Name plate NOTE CAUTION Figure 17 The plastics kit contains all parts but it is not assembled Observe the assembly instructions carefully The main cover cannot be disassembled once assembled incorrectly Insert the Agilent Technologies 1100 Series nameplate into the recess in the top cover 2 Place the top cover on the bench 3 Press the side panels into the slots in the top cover Assembling the Mai
154. th STEP 8 if you think you can adjust the torque otherwise proceed with STEP 9 Exchange the gripper arm assembly part number G1313 60010 Adjust the belt tension e If the measured torque value is too low the belt needs to be tightened e If the measured torque value is too high the belt needs to be loosened For this slide the motor X or theta on the holder bracket in the appropriate direction and test the tension with the torque2 macro Repeat this steps until the values are in the appropriate torque range Exchange the sample transport assembly part number G1313 60009 or G1329 60009 Exchange the main board part number G1313 69520 or G1329 69520 96 NOTE Step 1 Step 2 NOTE Step 3 NOTE Troubleshooting and Test Functions Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the via Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the via With Error messages LJ motor overtemp 0 or 2 L movement failed 0 or 2 When a motor over temperature message has occurred the sampler must be turned off for about 10 minutes to allow the motor to cool down Check the firmware and update to the latest revision if necessary Since firmware revision A 03 61 resident A03 60 most of following errors movement failed motor over temp and initialization failed X
155. the injection valve Connect column compartment inlet capillary to port 6 of the injection valve 3 Connect the corrugated waste tube to the solvent waste from the leak plane Ensure that the waste tube is positioned inside the leak channel Do not extend the waste capillary of the autosampler The siphoning effect might empty the complete seat capillary introducing air into the system Hydraulic Connections Pump outlet capillary T ji gpk a al a mii l AEN e a Waste tube in Leak channel il Solvent waste for corrugated waste tube E F Column compartment inlet capillary 31 Installing the Autosampler Sample Trays Sample Trays Installing the Sample Tray 1 Open the front door 2 Load the sample tray with sample vials as required 3 Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 4 Press the front of the sample tray down to secure the tray in the autosampler NOTE If the thermostatted autosampler tray pops out of position the air channel adapter is not correctly inserted Figure 11 Installing the Sample Tray 32 Table 6 Table 7 Installing the Autosampler Sample Trays Supported trays for the diff
156. ubleshooting and Test Functions Change Piston Change Piston The change piston function draws the piston away from the home position relieving the tension on the spring In this position the analytical head assembly can be removed and reinstalled easily after maintenance User Interface The commands for the ChemStation and Control Module different names for commands in the Control Module are shown in brackets are Start Change Draws the piston away from the home position relieving the tension on the spring End Done Repositions the plunger at the home position Using the Change Seal Function 1 Select Start Change to move the piston to the maintenance position 2 Exchange the metering seal see Gripper Arm on page 123 3 Select End Done to move the piston back to the home position 83 CAUTION NOTE Troubleshooting and Test Functions Park Arm Park Gripper Park Arm Park Gripper Before transporting or shipping the autosampler always secure the arm in the park position In order to prevent mechanical damage to the transport mechanism during transport the gripper arm should be moved into the park position The park arm function moves the gripper and transport slider to the home position behind the sampling unit and lowers the gripper arm into the park position where the transport assembly is secured against a mechanical stop The autosampler can be switc
157. ue screw caps 100 pk 5182 0717 Label halftray 5989 3890 Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 5063 6506 Finger caps x3 Tray for 40 x 2 ml vials Tray for 15 x 6 ml vials Capillary 0 17 mm 180 mm G1313 44502 G1313 44503 G1313 87305 Reorder gives pack of 15 18 Table 3 Installing the Autosampler Unpacking the Autosampler G1329A Thermostatted Autosampler Accessory Kit Contents G1329 68705 Description Tubing assembly Filter promo kit CAN cable 1 m long Screw cap vials clear 100 pk Blue screw caps 100 pk Label halftray Vial instruction sheet Wrenches 1 4 5 16 inch Rheotool socket wrench 1 4 inch Hex key 4 mm 15 cm long T handle Hex key 9 64 mm 15 cm long T handle Hex key 2 5 mm 15 cm long straight handle ESD wrist strap Finger caps x3 Front door cooled autosampler Air channel adapter Cover insulation Capillary 0 17 mm 900 mm Capillary heat exchanger Note for 1100 Series Autosampler door upgrade Part Number 5063 6527 5064 8240 5181 1519 5182 0714 5182 0717 5989 3890 no PN 8710 0510 8710 2391 8710 2392 8710 2394 8710 2412 9300 1408 5063 6506 G1329 40301 G1329 43200 no PN G1329 87300 01090 87306 no PN Reo
158. ul 61313 60007 61313 87303 5022 2133 G1313 87202 183 Parts and Materials External Tray G1313 60004 External Tray G1313 60004 Table 40 External Tray Item Description Part Number 1 External tray G1313 60004 2 Disposal tube 61313 27302 184 WARNING Table 41 Parts and Materials Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Cables Overview Type Description Part Number Analog 3390 2 3 integrators 01040 60101 cables 3394 6 integrators 35900 60750 Agilent 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remote 3390 integrator 01046 60203 cables 3392 3 integrators 3394 integrator 3396A Series integrator 3396 Series Il 3395A integrator see page 192 3396 Series Ill 3395B integrator HP 1050 modules HP 1046A FLD HP 1046A FLD Agilent 35900A A D converter 01046 60206 01046 60210 03394 60600 03396 61010 5061 3378 5061 3378 5061 3378 185 Parts and Materials Cable Overview Table 41 Cables Overview continued Type Description Part Number HP 1040 diode array detector 01046 60202 HP 1090 liquid chromatographs 01046 60202 Signal distribution module 01046 60202 BCD cables 3392 3 integrators 18594 60510 3396 integrator 03396 60560 General purpose spad
159. valve into the flow line for cleaning This switching event is performed at the end of the equilibration time to ensure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are used For example Outside wash of needle before injection 14 sec using flush port Injector program Draw x x y pl from sample Needle wash as method Inject Wait equilibration time see text above Valve bypass Wait 0 2 min Valve mainpass Valve bypass Valve mainpass 42 NOTE Optimizing Performance Optimization for Lowest Carry over Overlapped injection together with additional injection valve switching is not possible General Recommendation to Lowest Carry over e For samples where needle outside cannot be cleaned sufficiently with water or alcohol use wash vials with an appropriate solvent Using an injector program and several wash vials can be used for cleaning In case the needle seat has got contaminated and carry over is significantly higher than expected the following procedure can be used to clean the needle seat e Goto MORE INJECTOR and set needle to home position e Pipette an appropriate solvent on to the needle seat The solvent should be able to dissolve the contamination If this is not known use 2 or 3 solvents of different polarity Use several milliliters to clean the seat e Clean the needle seat with
160. ver and foam page 128 Li Ensure the transport assembly is seated firmly Li Verify the transport assembly alignment page 99 133 Repairing the Autosampler Sampling Unit _ _ Sampling Unit Frequency When accessing internal parts or when defective Tools required 1 4 inch wrench supplied in accessory kit Flat head screwdriver Parts required Sampling unit G1313 60008 for G1373A Sampling unit G1329 60008 for G1329A Sampling unit G1329 60018 for G1389A Sampling unit G2260 60008 for G2260A The sampling units come without injection valve and analytical head assembly see Sampling Unit Assembly on page 162 gt gt gt gt _ gt gt gt gt i F __ Before beginning this procedure 1 Remove the pump and column thermostat capillaries ports 1 and 6 from the injection 1 Remove the front cover valve Li Remove the vial tray Li Remove the top cover page 128 Li Remove the transport assembly page 132 134 Repairing the Autosampler Sampling Unit 2 Pull out the waste tube from the holder in the 3 Slide the sampling unit and tray base back to leak tray disengage the sampling unit connector 4 Lift the sampling unit and tray base approximately 10 cm Slide the leak sensor out of the leak plane 5 Lift the sampling unit and tray base out of the autosampler
161. ver kit for G7373A Autosampler Cover kit for G1329A G1389A G2260A include base side panels and top cover Name plate for Agilent 1100 Series Transparent front cover Door repair kit includes transparent side and front door on A Ww N Light protection kit includes opaque side and front door opaque front cover Cabinet upgrade kit includes side panels top cover transparent side and front door front cover and side insulation cover for cooled autosampler 61313 68703 61329 68703 0042 1381 G1313 68704 61329 68707 G1329 68708 61329 68706 Cover Parts 171 Parts and Materials Foam Parts Foam Parts Table 28 Foam Parts Item Description Part Number Foam kit includes items 2 and 3 61313 68702 5041 8395 Order foam kit Order foam kit 1 Board guides 2 Top foam 3 Bottom foam Figure 25 Foam Parts 172 Parts and Materials Power and Status Light Pipes Power and Status Light Pipes Table 29 Power and Status Light Pipes Item Description Part Number 1 Power switch button 5041 8381 2 Light pipe power switch 5041 8382 3 Power switch coupler 5041 8383 4 Light pipe status lamp 5041 8384 Figure 26 Power and Status Light Pipes 173 Parts and Materials Leak System Parts Leak System Parts Table 30 Leak System Parts Item Description Part Number 1 Leak funnel holder 5041 8389 2 Leak sensor 5061 3356 3 Leak plane 613
162. ver to press Done if the Serial box is blank In this case the module can no longer be recognized by either the control module or the ChemStation The main board must then be replaced Turn the autosampler off then on again The Records screen should display the correct serial number for this module If a ChemStation is also connected re boot the ChemStation now as well 150 NOTE Repairing the Autosampler ASM Board Entering the Serial Number using the ChemStation Module serial numbers are entered by typing specific commands into the command line at the bottom of the main user interface screen To enter a module serial number type the following command into the command line print sendmodule lals ser YYYYYYYYYY Where YYYYYYYYYY is the 10 character serial number of the module in question The first two characters are letters which should be capitalized The reply line will respond with RA 0000 SER followed by the module serial number you just entered Turn off the autosampler then on again Then re boot the ChemStation If the serial number you have just entered is different than the original module serial number you will be given the opportunity to edit the configure Agilent 1100 access screen during the re boot of the ChemStation After boot up the serial number you have just entered can be seen under the Instrument menu of the main user interface screen The serial number of the autosampler can
163. when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OF To disconnect the sampler from the line unplug the power cord The power will supply still uses some power even switch at the front panel is turned OFF 27 Installing the Autosampler Installing the Thermostatted Autosampler Installing the Thermostatted Autosampler Preparation Parts required Locate bench space Provide power connection Unpack the Sampler and the Thermostat Sampler and Thermostat Power cord for the other cables see below and Cable Overview on page 185 Chemstation and or Control Module G1323B WARNING Figure 7 Place the Thermostat on the bench Remove the front cover and route the condensation drain tube to the waste Make sure that the condensation tube is always above the liquid level in the vessel If the tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the instrument This may damage the instruments electronics Condensation Leak outlet Condensation drain tube Waste bottle E Install the LAN interface board in the sampler if required see Agilent 1100 Series Interface Board to general purposes on page 198 Remove the adhesive tape which covers the front door Remove the front door and
164. y Exchange the defective sampling unit motor Exchange the transport assembly Exchange the ASM board 72 Probable Causes Suggested Actions Troubleshooting and Test Functions Metering Home Failed Metering Home Failed The metering plunger has failed to move back to the home position The home position sensor on the sampling unit flex board monitors the home position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated e Dirty or defective sensor e Broken plunger e Defective metering drive motor e Defective ASM board Exchange the sampling unit flex board Exchange the metering plunger and seal Exchange the metering drive motor Exchange the ASM board OO O O 73 Probable Causes Suggested Actions Troubleshooting and Test Functions Motor Temperature Motor Temperature One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched off the motor to prevent damage to the motor See figure Transport Assembly on page 210 for motor identification Motor 0 temperature X axis motor Motor 1 temperature Z axis motor Motor 2 temperature Theta gripper rotation motor Motor 3 temperature Gripper motor motor for gripper fingers The processor monitors the current drawn by each motor and the time the motor i
165. zel Stator face Stator head aon oO A W W N Stator screws 0101 0921 0100 1852 0100 1853 0100 1849 0100 1851 0100 1850 1535 4857 Micro Injection Valve Assembly for G1389A The micro injection valve assembly has no ceramic stator face Item Description Part Number 1 Micro Inj valve assy incl items 1 2 3 5 6 2 Isolation seal 3 Micro rotor seal Vespel 5 Micro Stator head 6 Stator screws 0101 1050 0100 1852 0100 2088 0100 2089 1535 4857 168 Parts and Materials Injection Valve Assembly Table 25 Preparative Injection Valve Assembly for G2260A Item Description Part Number 1 MBB Injection valve assembly includes items1 6 0101 1267 2 Isolation seal 0100 1852 3 4 Rotor seal PEEK Stator face PEEK kit 0101 1268 5 Stator head 0100 2195 6 Stator screws 1535 4857 MBB Make Before Brake is a trademark by Rheodyne Figure 22 Injection Valve Assembly 169 Table 26 Figure 23 Parts and Materials Sheet Metal Kit Sheet Metal Kit Sheet Metal Item Description Part Number 1 Slot cover 2 Screw cover 3 Autosampler Sheet metal kit for G1313A Autosampler Sheet metal kit for G1329A G1389A G2260A 5001 3772 5022 2112 G1313 68701 G1329 68701 Sheet Metal Kit 170 Table 27 Figure 24 Parts and Materials Cover Parts Cover Parts Covers Item Description Part Number 1 Autosampler Co

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