Introduction to the Agilent 440 Fraction Collector
Contents
1. Performance Specifications Table2 Performance specifications Type Specification Number of fraction 1 3 racks Typical number of 21 200 tubes vial Maximal tube height 150 mm Available rack types 21 x 30 mmOD tubes 24x25 mm OD tubes lt 40x 20mm OD tubes 60x16 mm OD tubes lt 90x13 mm OD tubes 128 x15 mm OD 4 mL vials 200 x12 mm OD 2 mL vials 1 Optional overlays are available for the Type 40 and 60 racks These allow smaller diameter tubes to be used in the racks For example the overlay for the Type 40 racks enables the same rack for 20 mm OD tubes to be used for 18 mm OD tubes The overlays fit onto the top and center of the racks to hold the tubes securely in place The rack overlays for the fraction collector are also numbered to ensure the correct location of samples in the rack Note The rack overlays are designed for use with tubes that do not fit snugly into the racks If the fit of the tube is sloppy the probe may not center over the tube properly This can affect the accuracy of sample delivery and cause spills 14 440 Fraction Collector User Manual 440 Fraction Collector User Manual a i 16 i e Installation 6 e e e0 6 gt 7 e a e This chapter gives information about the installation of your instrument Agilent Technologies 3 Installation Installation For details on installation of the module refer to Agilent 218 Purification System2. Setup and Installation Guide p n G9300 90300 16 440 Fraction Collector User Manual 440 Fraction Collector User Manual a 7 Turning On the Instrument and Software 18 6 e 0 e6 6 7 e n e This chapter explains the operational parameters of the module Agilent Technologies 4 Using Turning On the Instrument and Software This procedure describes the basic steps to turn on the module and controlling software Preparations Ensure the module is connected via a three pin earthed power cord to a three pin grounded power outlet Module is setup and OpenLAB with drivers is installed on your computer for details refer to Agilent 218 Purification System Setup and Installation Guide p n G9300 90300 WARNING Hand injury or damage to objects The probe arm of the module can move without warning gt Do not place your hands or objects near the probe arm when the module is turned on 1 Turn on the power using the switch on the connection panel which is located on the rear of the control column The module will start up and perform an initialization sequence The initialization sequence moves the probe arm through the extent of the range of movement and finishes with the probe repositioned at the back left of the fraction collector If the module fails to initialize refer to Introduction to Troubleshooting and Diagnostics on page 24 2 Start your control software and navigate
3. specifications performance 14 physical 13 status indicators 25 status indicator code 32 symbols 68 T troubleshooting 440 Fraction Collector User Manual introduction 24 V voltage line 13 wW waste electrical and electronic equipment 73 WEEE directive 73 weight 13 440 Fraction Collector User Manual Index 79 www agilent com In This Book This manual covers the Agilent 440 Fraction Collector G9340A The manual describes the following e Introduction Site requirements and specifications Installation Using e Opimizing Troubleshooting e Errors e Maintenance Parts e Safety and the web Agilent Technologies 2012 Printed in Germany 02 12 G9340 90000 EE Agilent Technologies
4. 440 Fraction Collector User Manual Appendix 11 Inflammation or injury due to toxic corrosive or stimulative solvent gt Do not contact toxic corrosive or stimulative solvent gt For details of the properties of each solvent and how to handle it refer to the relevant Material Safety Data Sheets MSDS gt Be sure to handle each solvent properly gt Wear proper personal protective clothes e g safety goggles so that a solvent will not come into direct contact with the skin gt Ventilate the laboratory room adequately to prevent accidental inhalation of harmful solvent vapor Cuts gt When working with glass or quartz parts take care to prevent breakage Other Precautions Airflow to the cooling fans of the liquid chromatograph must be unobstructed Do not block the ventilation grills on the liquid chromatograph and accessories Consult the manuals supplied with your PC monitor and for their specific ventilation requirements Moving Parts Hazard Personal injury The probe arm of the module can move without warning posing a potential hazard due to its moving parts gt Always switch off the power to the module before installing or removing parts 440 Fraction Collector User Manual 71 11 Appendix Flammable liquids WARNING See The module consumes power produces heat or conducts heat from other areas gt Use only flammable liquids with a flash point gt 25 C above ambient tempe
5. 57 Sample Racks 58 Rack Overlays 59 Tubing and Probes 60 Miscellaneous Spares 61 Fuses 62 10 Cables 63 Cable Overview 64 Cable Connections 65 Backpanel Overview 66 11 Appendix 67 General Safety Information 68 The Waste Electrical and Electronic Equipment Directive 73 Radio Interference 74 CECompliance 75 Electromagnetic Compatibility 76 Agilent Technologies on Internet 77 6 440 Fraction Collector User Manual 440 Fraction Collector User Manual a JNE r Introduction to the Agilent 440 Fraction Collector 8 This chapter gives a module overview Agilent Technologies 1 introduction Introduction to the Agilent 440 Fraction Collector The Agilent 440 Fraction Collector is a random access single probe fraction collector and can accommodate a variety of racks It is designed to automate the sample collection process This fraction collector is designed to meet the diverse requirements of high throughput laboratories Three racks are included with the fraction collector and are made of polypropylene to resist most chemical spills Up to three racks of many configurations can be placed on the fraction collector Additional sample racks can be set up in sequence and manually changed during an analysis as each rack s analysis is completed The rack closest to the rear of the fraction collector next to the pillar is considered rack number one 8 440 Fraction Collector User Manual 440 Fraction Collec
6. An operator intervention is required Red A danger or an emergency is present 440 Fraction Collector User Manual 25 6 Troubleshooting and Diagnostics Quick Troubleshooting Guide 26 Table 4 Troubleshooting Quick Guide Symptom Possible cause Solution Fractions not correlating to tick marks in OpenLAB software Liquid not delivered to the tubes Probe hits top of tubes or is not low enough Arm not moving correctly in the X theta or Z axes Delay volume incorrect Clogged tubing Clogged probe Sticking valve Probe depth not correctly set Wrong rack chosen Wrong tubes being used Motor not correctly operating Determine the correct delay volume and enter it into the System Parameters or software For details refer to help of the control software Check the tubing for blockages and replace it if necessary See Replacing the Tubing on page 53 Clean the probe See Cleaning the Probe on page 46 Replace the probe See Replacing the Probe and Probe Tubing on page 50 Replace the valve Contact your Agilent representative Set the probe depth For details refer to help of the control software Choose the correct rack in System Parameters or the software see the ChemStation help Replace with the correct tubes Run the Reset procedure See Reset Procedure on page 29 Run the Self test procedure See Self Test Sequence on page 30 C
7. capacity Sample Rack Type 200 Capacity no tubes x OD 200 x 12 mm 2 mL capacity 440 Fraction Collector User Manual Rack Overlays p n Description 6610026000 Rack Conversion for Type 40 rack 18 mm OD holes 6610026100 Rack Conversion for Type 60 rack 16 mm OD holes 440 Fraction Collector User Manual Parts 9 59 9 Parts Tubing and Probes 60 p n 9910130800 9910142900 9910137700 9910138200 Description Probe kit with 0 050 cm 0 020 in ID stainless steel probe includes 0 025 cm 0 010 in ID tubing High Flow Probe Kit Funnel Rack Kit Funnel Rack HiFlow Probe and Valve Kit 440 Fraction Collector User Manual Parts Miscellaneous Spares p n 3710051100 6610025100 9910056300 8010 0198 1910009100 1910010700 R007200141 110744200 210187590 5140892700 7910062900 Description Polypropylene tubes 16 mm OD x 125 mm height bag of 125 tubes Fraction collector spill tray Trolley cart for the fraction collector Features lockable wheels and lower shelf for storage 2 mL screw top wide opening clear glass vials 12 x 32 mm 9 mm red caps with PTFE Silicone liners 100 per pack Fuse 5 A 440 LC FC Fuse 3 15 A 440 LC FC 3 wire LC interconnect cable for most connections LC analog signal cable 325 335 Relay Interface Board 210 218 Rear Back Panel Interface Approved USB to RS232 Converter 440 Fraction Collector User Manual 9 61 9 Parts Fuses For safety r
8. different setup to avoid high back pressure gt 0 50 mL min Standard probe and valve gt 50 400 mL min High flow probe possible funnel rack gt 400 800 mL min High flow probe HiFlow valve and funnel rack gt Do not use the 0 010 in tubing when operating at flow rates 22 mL min Remove the tubing from inside the probe a Slide the black piece of tubing completely onto the stainless steel probe tube The black tubing secures the Tefzel tubing into the metal probe tube b Pull the tubing out of the probe if you are replacing the tubing This tubing will be used as a guide for the new tubing length Cut the supplied 0 020 in 0 50 mm ID Tefzel or narrow bore tube 0 010 in ID 0 25 mm ID tubing to a length equal to the tubing previously removed from the probe Slide a new Nut short 1 16 in flangeless ETFE Tefzel and then a new Ferrule 1 16 in flangeless natural ETFE Tefzel onto one end of the tubing Slide the 0 020 in 0 50 mm ID or the 0 010 in 0 25 mm ID low delay volume Tefzel tubing into the stainless steel probe if you are replacing the tubing inside the probe Push the tubing down into the probe so that at least 0 5 cm tubing protrudes from the end of the probe Slide the black tubing so that half of the tubing is on the metal probe and half on the Tefzel tubing This secures the tubing to the metal probe 7 Slide the probe into the probe mounting block 8 Fit and tighten the lower
9. intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Symbols 68 The following is a list of symbols that appear with warnings in this manual or on the liquid chromatograph The hazard they describe is also shown A triangular symbol indicates a warning The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows 440 Fraction Collector User Manual Appendix 11 Table 6 Warning symbols Chemical hazard Heavy weight danger to feet Moving parts AA Electrical Fire hazard shock A A Heavy weight Inhalation danger to hazard hands Warning label The following symbol may be used on warn ing labels attached to the instrument When you see this symbol refer to the relevant op eration or service manual for the correct pro cedure referred to by that warning label Table 7 Information Symbols Symbol l 0 WO NA CE Description Mains power on Mains power off Fuse Single phase alternating current Direct current When attached to the rear of the instrument indicates that the product complies with the requireme
10. of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 440 Fraction Collector User Manual 11 2 Site Requirements and Specifications Area selected WARNING Explosion damage and accuracy of the module gt Select an area free from drafts corrosive atmospheres and vibration gt Select a dust free low humidity environment gt Use air conditioning for control of the environment Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where tem
11. Agilent 440 Fraction Collector User Manual tt Agilent Technologies Notices Agilent Technologies Inc 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 69340 90000 Edition 02 12 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Ag
12. B 001 This ISM device complies with Canadian ICES 001 Cet appareil ISM est conforme a la norme NMB 001 du Canada 440 Fraction Collector User Manual Appendix 11 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 440 Fraction Collector User Manual 71 Index Index A Agilent on internet 77 ambient operating temperature 13 backpanel 66 bench space 12 C cleaning general 45 rackmat 47 spill tray 47 code LED 32 communication error 35 compliance CE 75 condensation 12 connections workstation control 65 D diagnostics introduction 24 dimensions 13 E electronic waste 73 error code 32 78 communication 35 probearm 34 F frequency line 13 fuse replace 54 fuses 62 hazard moving parts 71 humidity 13 initialization process 36 internet 77 introduction 8 L liquids flammable 72 M maintenance routine 44 0 operating altitude 13 operating temperature 13 optimizing 22 P parts miscellaneous spares 61 rack overlay 59 sample racks 58 performance optimizing 22 power consumption 13 power cords 11 probe arm error 34 radio interference 74 reset 29 S safety general information 68 standards 13 self test 30 site requirements power cords 11
13. The black tubing secures the Teflon tubing into the metal probe tube 10 If you are replacing the tubing pull it out of the probe and save it This tubing will be used as a guide for the new tubing length 440 Fraction Collector User Manual 49 8 Maintenance Replacing th tubing securing the tubing in the metal probe rled mount nut on the probe assembly Preparations 50 e Probe and Probe Tubing Follow this procedure to insert either the standard 0 020 in or low flow 0 010 in tubing into the probe and then install the assembly on the fraction collector If you are installing High Flow Probe Kit p n 9910142900 please see Instruction sheet p n 8510250500 that came with it to assemble and install the hardware Use the low delay volume tubing for operation of the fraction collector at or below 1 mL min or collection of very narrow lt 30 s wide peaks for good results The Low delay volume tubing Tefzel 1 16 in OD by 0 010 in ID comes with Probe kit with 0 050 cm 0 020 in ID stainless steel probe includes 0 025 cm 0 010 in ID tubing p n 9910130800 al Figure 2 Probe details If already installed on the fraction collector remove the probe assembly See Removing the Probe from the Fraction Collector on page 48 440 Fraction Collector User Manual Maintenance 8 CAUTION Damage to flow cell upstream Back pressure may damage detector flow cell upstream Different flow ranges require
14. ashes this indicates that the fraction collector has received a message from the instrument computer It will always respond on receipt of the Enter key regardless of the success of the message this will indicate the Baud Rate is correct 5 If there are no flashes confirm that the fraction collector is functioning by watching for the CPU Activity LED to flash Refer to Figure 4 on page 66 for the location of this LED This should take about 10 15 s If this does not occur reset the fraction collector to ensure that it follows through the reset sequence and then wait until the CPU Activity LED flashes If this is successful then retest from step 4 above 6 If unsuccessful contact your Agilent representative 440 Fraction Collector User Manual 35 7 Error Information Failure to Initialize The fraction collector initialization process is a powerful self diagnostic tool The initialization procedure is as follows On power up the autosampler runs through the initialization sequence and sets the probe position The probe moves to all the physical limits of the working envelope of the fraction collector and then returns to the home position at the drain This initialization sequence will be performed each time the fraction collector is switched on The probe travels through the following sequence e Probe raises to the full extreme of the Z axis e Probe travels to the full extremes of the X axis e Probe travels to the full extrem
15. dicator is faulty Further confirmation of a failure can be confirmed by observing the status of the other LED indicators the communications and by resetting the fraction collector by powering it off and then on If it does not perform the initialization sequence by driving the probe arm there is a fault Contact your Agilent representative Contact your Agilent representative 440 Fraction Collector User Manual 27 6 28 Troubleshooting and Diagnostics Table 4 Troubleshooting Quick Guide Symptom Possible cause Solution Fraction collector is Probe arm or probe Turn the power off to the fraction collector not initializing unable to move tothe Check for mechanical obstructions correct positions Turn the fraction collector on to allow it to Error status LEDs show an error attempt the reset procedure Watch where the fraction collector fails during the initialization procedure See Failure to Initialize on page 36 e Contact your Agilent representative See Error Status Indicator Codes on page 32 440 Fraction Collector User Manual Troubleshooting and Diagnostics 6 Reset Procedure The reset procedure identifies problems with the movement of the autosampler in the X and Theta axes Tools required Description Small pointed device 1 Depresse the recessed button see Figure 4 on page 66 If the unit successfully completes the reset procedure then the motor s
16. e probe to the HPLC system is secure and kink free 2 Check the probe and tubing for blockages 3 Clean any spills by removing draining and rinsing the spill tray 440 Fraction Collector User Manual Maintenance 8 General Cleaning Cleaning should be performed using warm water and a soft lint free cloth This will help preserve the plastic components and painted surfaces Tools required Description Soft lint free cloth Warm water 1 Wipe up any spills immediately 2 Pump arinse solution through the fraction collector at the end of a run This avoids clogging the probe and tubing 3 If necessary remove the probe from the fraction collector and place it in an ultrasonic bath for cleaning 440 Fraction Collector User Manual 45 8 Maintenance Cleaning the Probe Tools required Description Washing solution compatible with your HPLC system and current solvent Ultrasonic bath 1 Run washing solution compatible with your HPLC system and current solvent through the fraction collector tubing OR If it is completely blocked replace the tubing 2 Sonicating the probe and tubing Sonicating may help clean any particulates out of the tubing OR If sonicating the probe and tubing assembly does not remove the blockage replace it 3 For information on how to remove and replace the probe see Replacing the Probe and Probe Tubing on page 50 46 440 Fraction Collector User Manual Maintenance 8 Cleaning t
17. easons any other internal fuse or circuit breaker is not operator accessible and should only be replaced by Agilent authorized personnel Fuse information on the rear of the instrument is the most up to date p n Description 2x Fuse 250 V 5 A 5 x20 mm M205 62 440 Fraction Collector User Manual 440 Fraction Collector User Manual 2e0 eece 10 66 Cables 6 e ii e j Cable Overview 64 s Cable Connections 65 Backpanel Overview 66 This chapter provides information on cables used with the module ee Agilent Technologies 63 10 Cables Cable Overview Necessary cables p n Description 392612901 Ethernet cable for use in a network 5023 0203 Ethernet cable cross over for standalone use 392607969 Inject marker cable 392607975 Next injection cable 393546291 Serial communication ribbon 393597601 Converter RS232 to RS422 7910046300 Serial cable Optional cables p n Description 110743800 Relay interface cable for relay interface board one relay contact per cable 110744200 Analog signal cable 64 440 Fraction Collector User Manual Cables 10 Cable Connections Cable Connections Back connectors for 218 to internal CIM 9 Pin connectors Serial cable communication a Converter RS 232 t RS ooo d SS Ethernet GPIB ar Next injection 218 Pump 410 Autosampler S 325 Detector e Inject marker cable 440 Fraction lel U cPu Fi
18. es of the Theta axis e Probe positions itself at the back left of the fraction collector If the fraction collector fails to initialize the probe arm has not travelled the required distance along one or all of the axes One or more LEDs will light to indicate an error 36 440 Fraction Collector User Manual Error Information 7 External DIP Switches External DIP Switches NOTE All external DIP switches should be in the OFF position 440 Fraction Collector User Manual 37 7 Error Information External DIP Switches 38 440 Fraction Collector User Manual 440 Fraction Collector User Manual 8 Maintenance Introduction to Maintenance 40 Safety Information 42 Routine Maintenance 44 General Cleaning 45 Cleaning the Probe 46 Cleaning the Spill Tray and Rack Mat 47 Removing the Probe from the Fraction Collector 48 Replacing the Probe and Probe Tubing 50 Replacing the Tubing 53 Replacing the Fuse 54 This chapter describes the maintenance of the module oe Agilent Technologies 39 8 Maintenance Introduction to Maintenance Introduction to Maintenance NOTE This chapter includes the maintenance requirements that may be carried out by an operator Any maintenance procedures not specifically mentioned in this chapter should be carried out only by Agilent trained Agilent qualified or Agilent authorized representatives Ro 40 Figure 1 Fraction collector components 440 Fraction Col
19. ey are used to provide a visual indicator of an error that has occurred with the fraction collector Other more extensive error reporting is available via the software commands The error indicators illuminate to show the binary error code for the fault E4 is the least significant bit in the binary code If an error occurs the binary error code is indicated and an error message is sent to the OpenLAB software for example Error 7 0111 Theta axis position error Refer to the table below for the error indicator codes 440 Fraction Collector User Manual Table 5 Error status indicator codes Error Information 7 E1 E2 E3 E4 Error Error description Solution number 0 0 0 0 0 No Error 0 0 0 1 1 Reserved 0 0 1 0 2 Program memory check sum error Contact your Agilent representative 0 0 1 1 3 Configuration memory check sum error Contact your Agilent representative 0 1 0 0 4 RAM Test error Contact your Agilent representative 0 1 0 1 5 Reserved 0 1 1 0 6 X axis position error The X axis motor was Troubleshoot the probe arm commanded to move but did not reach the desired position 0 1 1 1 7 Theta axis position error The Theta axis motor was commanded to move but did not reach the desired position 1 0 0 0 8 Z axis position error The Z axis motor was commanded to move but did not reach the desired position 1 0 0 1 9 Reserved 1 0 1 0 10 Reserved 1 0 1 1 11 X axis movement detected without the motor being commanded t
20. gure 3 Cable connections for workstation control of Agilent 218 Pumps Agilent 325 Detector Agilent 410 Autosampler and Agilent 440 Fraction Collector 440 Fraction Collector User Manual 65 10 Cables Backpanel Overview Error indicators l 1234 Error umun Reset button Revet Dip switches DIP A DIP A Relay status indicators showing the status of relays A B qnd 0 which are used tor the hardwired 1 0 circuit oard Console software configuration Status indicators Auxiliary a D i communication port not supported nstrument communication port Power supply inlet switch and fuses Figure 4 Connection panel 440 Fraction Collector User Manual 440 Fraction Collector User Manual 11 Appendix General Safety Information 68 The Waste Electrical and Electronic Equipment Directive 73 Radio Interference 74 CE Compliance 75 Electromagnetic Compatibility 76 Agilent Technologies on Internet 77 This chapter provides addition information on safety legal and web ee Agilent Technologies 67 11 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and
21. han domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purposes This device complies with the requirements of CISPR11 Group 1 Class A as radiation professional equipment Therefore there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances Operation is subject to the following two conditions This device may not cause harmful interference e This device must accept any interference received including interference that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures 1 Relocate the radio or antenna 2 Move the device away from the radio or television 3 Plug the device into a different electrical outlet so that the device and the radio or television are on separate electrical circuits 4 Make sure that all peripheral devices are also certified 5 Make sure that appropriate cables are used to connect the device to peripheral equipment 6 Consult your equipment dealer Agilent Technologies or an experienced technician for assistance 7 Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment ICES NM
22. he Spill Tray and Rack Mat Turn off the fraction collector Remove the racks Lift up and remove the rack mat Carefully lift up and remove the spill tray Clean the spill tray and rack mat ao a A Q N Dispose of any waste in the appropriate manner 440 Fraction Collector User Manual 47 Maintenance Removing the Probe from the Fraction Collector Removing the Probe from the Fraction Collector Turn off the fraction collector Slide the probe assembly to the top of the probe carriage Manually rotate the probe arm so that it can be easily accessed 4 Unscrew the tubing from the divert valve position 3 Port 1 to waste Port 2 from detector outlet or back pressure restrictor Port 3 to the probe Remove the tubing from the tube restraint 6 Remove the tubing from the split retaining ring at the underside of the housing Split ring retaining ring 48 440 Fraction Collector User Manual Maintenance 8 7 Disassemble the probe by unscrewing the nut holding the metal probe from the bottom of the knurled mount and then remove the lower knurled mount nut that retains the knurled mount Top knurled mount on the probe assembly Lower knurled mount nut on the probe assembly Nut holding the metal probe Next Steps 8 Lift the probe and tubing out of the probe mounting block 9 Slide the black piece of tubing completely onto the stainless steel probe tube
23. ilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per for
24. is of the correct type and that it is not damaged If necessary replace the fuse s in the holder 440 Fraction Collector User Manual 55 8 Maintenance 5 Ensure each fuse is held securely by the prongs inside the holder If the contact is too loose remove the fuse from the holder and then use a pair of pliers to compress the distance between the prongs so as to tighten their grip on the fuse Replace the fuse Next Steps 6 Slide the fuse compartment back into the compartment and clip the cover back into place ensure that it clicks into the locked position 7 Reconnect the power supply cable and switch on power to the unit 56 440 Fraction Collector User Manual This chapter provides information on parts for the instrument 440 Fraction Collector User Manual 9 Parts Sample Racks 58 Rack Overlays 59 Tubing and Probes 60 Miscellaneous Spares 61 Fuses 62 ee Agilent Technologies 57 9 Parts Sample Racks 58 p n 6610026600 6610026500 6610025500 6610025400 6610026400 7910056000 7910060100 Description Sample Rack Type 21 Sample Rack Type 24 Sample Rack Type 40 Sample Rack Type 60 Sample Rack Type 90 Capacity no tubes x OD 21 x 30 mm Capacity no tubes x OD 24 x 25 mm Capacity no tubes x OD 40 x 20 mm Capacity no tubes x OD 60 x 16 mm Capacity no tubes x OD 90 x 13 mm Sample Rack Type 128 Capacity no tubes x OD 128 x 15 mm 4 mL
25. knurled nut holding the probe to the top knurled mount nut Fit and tighten the nut to the lower knurled mount 10 Clip the tubing into the tube restraint in the Z axis slider Allow for a little extra length in the tubing to prevent kinking 440 Fraction Collector User Manual 51 52 Maintenance 11 Feed the tubing through the retaining ring at the underside of the housing The retaining ring is split to allow you to slide the tubing into it 12 Screw the tubing fitting into port 3 of the valve The length of the tubing must allow the probe arm to move freely in all axes If it is too tight it will restrict movement and may cause movement failure If it is too loose the probe arm may become tangled in the tubing as it moves It is also important to keep the tubing length to a minimum to avoid a large delay volume 13 Move the probe arm to all four corners of the fraction collector while rotating the probe arm fully to check that the tube is completely free to move It is also important to keep the minimum length of tubing to minimize the delay volume 14 Manually position the probe arm at the midpoint along the X axis 15 Rotate the probe arm through its full extent to check that the tubing is free to allow full movement in all directions 16 If you are replacing the tubing from the divert valve to the back pressure regulator or detector a Remove the tubing from port 2 on the divert valve and the back pressure regulato
26. lector User Manual Maintenance 8 1 Theta axis 2 Z axis slide 3 Top knurled mount nut on the probe assembly 4 Probe mounting block 5 Lower knurled mount nut on the probe assembly Nut holding the metal probe 7 Probe 8 Rack 9 Rack location mat 10 Split retaining ring 11 Fraction collector pillar control column If you have purchased Funnel Rack Kit p n 9910137700 or Funnel Rack HiFlow Probe and Valve Kit p n 9910138200 or High Flow Probe Kit p n 9910142900 please see Instruction sheet p n 8510250500 that came with it for instructions on how to assemble and install the hardware onto your fraction collector 440 Fraction Collector User Manual 41 8 Maintenance Safety Information Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover i
27. med or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 440 Fraction Collector User Manual In This Book In This Book This manual covers the Agilent 440 Fraction Collector G9340A 1 Introduction This chapter gives a module overview 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installation This chapter gives information about the installation of your instrument 4 Using This chapter explains the operational parameters of the module 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 8 Maintenance This chapter describes
28. nts of one or more EU directives 440 Fraction Collector User Manual 69 11 Appendix Solvent Hazards WARNING Explosion fire asphyxiation This instrument is not explosion proof Certain solvents may cause weakening and leaks of tubings or fitthings with possible bursting Even small leaks in solvent supply systems can be dangerous gt Only use solvents compatible with the HPLC system tubings and fittings gt Employ static measuring and static discharge devices to safeguard against the buildup of static electricity gt In unattended operation do not use organic solvents having an ignition point below 70 C gt Do not bring a heat or flame source near the instrument gt The area in which solvents are stored and the area surrounding the instrument must be adequately ventilated to prevent accumulations of gas gt Always check the condition of the instrument leakage of solvent or waste solution leakage of solvent inside the instrument If an abnormality is found stop operation immediately gt When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste gt Use only approved regulator and hose connectors refer to the supplier s instructions gt Keep solvents cool and properly labeled Ensure that you have the correct solvent before connecting it to the instrument 70
29. o move 1 1 0 0 12 Theta Axis movement detected without the motor being commanded to move 1 1 0 1 13 Z axis movement detected without the motor being commanded to move 1 1 1 0 14 Reserved 1 1 1 1 15 Reserved Troubleshoot the probe arm Troubleshoot the probe arm Probe arm moved See Probe Arm Error on page 34 Probe arm moved See Probe Arm Error on page 34 Probe arm moved See Probe Arm Error on page 34 440 Fraction Collector User Manual 33 7 Error Information Probe Arm Error 34 A probe arm error is indicated when the fraction collector cannot complete its instructed move This could be due to the e Probe arm hitting an obstacle while moving into position e Probe arm not reaching a position within the defined limits of the intended position This causes the probe movement to cease Movement errors of less than 1 mm are enough to cause a probe jam e Mechanical movement being abnormally stiff making it difficult for the probe arm to move The probe drive will cease e Arm being manually moved In all cases if an error occurs the error indicator LEDs will show the error code and an error message will be sent to the computer If the probe arm becomes jammed the error control system will lock the motor drive and send an error message to the computer and to the error indicator LEDs If this happens re initialize the fraction collector power off wait 10 seconds power
30. on and then watch for correct initialization If the probe line has been squashed as a result of the probe arm jam the tubing should be replaced to prevent low sample flow or carryover If there is no obvious cause for the jam contact your Agilent representative 440 Fraction Collector User Manual Error Information 7 Communications Error The RS232 LED flashes when serial communications from the client computer are received Refer to Figure 4 on page 66 for the location of this LED Use the computer to send software commands to the fraction collector If the LED flashes the internal computer is operating and it is receiving commands from the HPLC system computer and its power supply is operational The flashing only indicates that a character has been received It does not indicate that the command is correct If the fraction collector shows offline in the Status Overview or won t connect to the Console software follow these steps to troubleshoot 1 Check that the RS232 cable between the HPLC system or computer and fraction collector is firmly connected and screwed into place 2 Initialize the fraction collector to ensure that it is in a working state Turn off the power switch wait 10 seconds and turn the power switch on 3 When the software is started the software lights the RS232 LED linked to the RS232 connector 4 Check that the RS232 LED flashes while commands are being sent to the fraction collector If there are fl
31. ontact your Agilent representative if a test fails 440 Fraction Collector User Manual Table 4 Troubleshooting Quick Guide Troubleshooting and Diagnostics 6 Symptom Possible cause Solution Fraction collector shows offline in Status Overview Green indicator on the front of the fraction collector is off CPU activity LEDS on the rear of the fraction collector are off Fraction collector is turned off Fraction collector is on but not communicating Indicator may be faulty Fuse has blown No mains power Faulty internal power supply Faulty indicator Faulty main electronics board Faulty internal power supply Turn on the fraction collector Check the power cable connections Check the fuses If blown contact your Agilent representative Check the RS232 cable connections lt Reboot the fraction collector Troubleshoot communication See Communications Error on page 35 lt Check the LEDs See Error Status Indicator Codes on page 32 Turn off the fraction collector Wait 10 seconds before turning the unit on again If the unit initializes but the light does not come on then the indicator is faulty Contact your Agilent representative Check the mains supply Contact your Agilent representative Turn off the fraction collector Wait 10 seconds before turning the unit on again If the probe arm initializes but the light does not come on then the in
32. operation and the action of the carriages is correct 440 Fraction Collector User Manual 29 6 Troubleshooting and Diagnostics Self Test Sequence The self test sequence tests that the fraction collector functions in X Theta and Z axes by moving through the standard and sample positions for the currently loaded rack settings It does not test the serial communications 1 Turn off the power switch wait 10 seconds and turn the power switch on The module runs the self test automatically on starting up If the self test sequence is successful it is only necessary to check the serial port connection to the instrument computer 30 440 Fraction Collector User Manual 440 Fraction Collector User Manual 7 Error Information Error Status Indicator Codes 32 Probe Arm Error 34 Communications Error 35 Failure to Initialize 36 External DIP Switches 37 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions ee Agilent Technologies 31 7 Error Information Error Status Indicator Codes 32 Error indicators have been provided to give a quick view of the status of the fraction collector and of the possible cause of incorrect functioning of the unit Refer to Figure 4 on page 66 for the location of the error indicator LEDs The error indicators are a line of blue LEDs on the connection panel of the fraction collector Th
33. perature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation Bench Space The module dimensions and weight see Table 1 on page 13 allow you to place the module on almost any desk or laboratory bench It needs an additional 5 cm 2 in of space on either side and approximately 15 cm 5 9 in at the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position 12 440 Fraction Collector User Manual Physical Specifications Table 1 Physical specifications Site Requirements and Specifications 2 Type Specification Weight 18 kg 34 Ib Dimensions height x width x depth 510 mm x 490 mm x 285 mm Line voltage Line frequency Power consumption Ambient operating temperature Operating humidity RH non condensing Operating altitude Safety standards EN 61010 1 IEC CSA UL 21 in x 20 in x 12 in 1 5 7 1 8 0 00 240 VAC tested 90 264 VAC 0 60 Hz 1 Hz 5W 100VA 0 35 C 50 95 F 80 3050 m 0 10000 ft Installation category Pollution degree 2 Equipment class 440 Fraction Collector User Manual 13 2 Site Requirements and Specifications
34. r or detector b Cut the supplied 0 020 in 0 50 mm ID Tefzel or narrow bore tube 0 010 in ID 0 25 mm ID tubing to a length equal to the tubing previously removed in step a on page 52 It is important to keep the length of tubing short as possible to minimize the volume delay c Slide a new Nut short 1 16 in flangeless ETFE Tefzel and then a new Ferrule 1 16 in flangeless natural ETFE Tefzel both also supplied with the probe kit onto one end of the tubing Screw the tubing fitting into port 2 of the valve d Slide the appropriate nut and ferrule for the back pressure regulator or detector output onto the other end Screw the tubing fitting into the detector outlet or back pressure restrictor if one is present on your system 17 Determine the volume delay and probe depth For details refer to help of control software You will need to reset the volume delay in software 440 Fraction Collector User Manual Maintenance 8 Replacing the Tubing Use this procedure to replace the tubing from the divert valve to the detector or the divert valve to the waste 1 2 3 4 oo N OO Turn off the flow Open the purge valve to release any residual pressure Unscrew the fitting from the valve at port 1 or 2 See Figure on page 48 Unscrew the fitting at the detector outlet or back pressure restrictor if applicable Replace the tubing and or fittings as needed Tubing 0 020 in or 0 010 in ID 1 16 in OD Nut shor
35. rature gt Do not allow flammable liquids to come near to any heat emiting part of the module gt Keep anyting capable of igniting a flammable liquid well away from the module 72 440 Fraction Collector User Manual Appendix 11 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 440 Fraction Collector User Manual 73 11 Appendix Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the
36. s opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 42 440 Fraction Collector User Manual Maintenance 8 CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment Moving Parts iim The probe arm of the module can move without warning posing a potential hazard due to its moving parts gt Always switch off the power to the module before installing or removing parts gt Keep away from the probe while collecting samples or moving the probe arm 440 Fraction Collector User Manual 43 Maintenance Routine Maintenance 44 There are no parts that require frequent maintenance However always take the following cleaning measures 1 Confirm that the tubing from th
37. t 1 16 in flangeless ETFE Tefzel Ferrule 1 16 in flangeless natural ETFE Tefzel Tighten the nuts to finger tight Close the purge valve Turn on the flow and check for leaks Determine the volume delay For details refer to help of control software 440 Fraction Collector User Manual 53 8 Maintenance Replacing the Fuse Replace a blown fuse with one of the same type and rating Parts required Description Fuse 250 V 5 A 5 x20 mm M205 Always check the information printed on the back of the fraction collector for the correct fuse type as this is the most up to date CAUTION Wrong fuse Damage to the module and loss of warranty gt Set voltage properly gt Use only the correct fuses WARNING Electrical shock gt Always disconnect power cord from the module before replacing a fuse 54 440 Fraction Collector User Manual Maintenance 1 Disconnect the module from the mains power supply 2 Use a thin flat blade screwdriver or similar tool to pry open the cover of the fuse compartment which is located on the power supply unit near the bottom of the connection panel on the rear of the module E wy Flat blade screwdriver Cover of fuse compartment 100 240V 10 75W 50 60Hz 1Hz 5x20mm Correct fuse information 3 Slide out the fuse holder from the compartment Flat blade screwdriver Cover of fuse compartment 4 Check that each fuse
38. the maintenance of the module 440 Fraction Collector User Manual 3 In This Book 9 Parts This chapter provides information on parts for the instrument 10 Cables This chapter provides information on cables used with the module 11 Appendix This chapter provides addition information on safety legal and web 4 440 Fraction Collector User Manual Contents Contents 1 Introduction 7 Introduction to the Agilent 440 Fraction Collector 8 2 Site Requirements and Specifications 9 Site Requirements 10 Physical Specifications 13 Performance Specifications 14 3 Installation 15 Installation 16 4 Using 17 Turning On the Instrument and Software 18 5 Optimizing Performance 21 Optimizing Performance 22 6 Troubleshooting and Diagnostics 23 Introduction to Troubleshooting and Diagnostics 24 Status Indicators 25 Quick Troubleshooting Guide 26 Reset Procedure 29 Self Test Sequence 30 7 Error Information 31 Error Status Indicator Codes 32 Probe Arm Error 34 Communications Error 35 Failure to Initialize 36 External DIP Switches 37 440 Fraction Collector User Manual Contents 8 Maintenance 39 Introduction to Maintenance 40 Safety Information 42 Routine Maintenance 44 General Cleaning 45 Cleaning the Probe 46 Cleaning the Spill Tray and Rack Mat 47 Removing the Probe from the Fraction Collector 48 Replacing the Probe and Probe Tubing 50 Replacing the Tubing 53 Replacing the Fuse 54 9 Parts
39. to the relevant sections for details refer to Agilent 218 Purification System Setup and Installation Guide p n G9300 90300 or the help of the control software 18 440 Fraction Collector User Manual Using Following further items are constitutive to run the module For detailed information on these items please refer to the help of the control software Creating methods Setting up the fraction collector for sample collection Information logs Determining the volume delay and Determining the correct probe depth 440 Fraction Collector User Manual 19 4 Using Turning On the Instrument and Software 20 440 Fraction Collector User Manual 440 Fraction Collector User Manual a 22 i g Optimizing Performance o a o e e6 6 7 e oe Performance e e This chapter gives hints on how to optimize the performance or use additional devices Agilent Technologies 21 5 Optimizing Performance Optimizing Performance Optimizing the performance involves following steps e Determining the volume delay e Minimizing the volume delay e Running a probe alignment depending on the result of the check For details on these procedures refer to the help of the control software 22 440 Fraction Collector User Manual 440 Fraction Collector User Manual 6 Troubleshooting and Diagnostics Introduction to Troubleshooting and Diagnostics 24 Status Indicators 25 Quick Troubleshooting G
40. tor User Manual ee 2 Site Requirements and Specifications oe e e e e ii e j Site Requirements 10 Physical Specifications 13 Performance Specifications 14 This chapter provides information on environmental requirements physical and performance specifications ti Agilent Technologies 9 2 Site Requirements and Specifications Site Requirements Power Considerations The instrument power supply has wide ranging capability It accepts any line voltage in the range described in Physical Specifications Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 10 440 Fraction Collector User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence
41. uide 26 Reset Procedure 29 Self Test Sequence 30 This chapter gives an overview about the troubleshooting and diagnostic features ee Agilent Technologies 23 6 Troubleshooting and Diagnostics Introduction to Troubleshooting and Diagnostics 24 This is a quick guide to identifying and correcting possible problems with your fraction collector The list of problems is not exhaustive so if you cannot correct the problem using the information provided you should contact your Agilent representative Information is provided about e Fractions not correlating to the tick marks on the OpenLAB software e Liquid is not delivered to the tubes e Probe hits the top of the tubes or is not low enough The arm is not moving correctly in the X theta or Z axes e The fraction collector shows offline in Status Overview e The green indicator on the front of the fraction collector is off The CPU activity LEDS on the rear of the fraction collector are off e Fraction collector is not initializing e Error status LEDs are showing an error 440 Fraction Collector User Manual Troubleshooting and Diagnostics 6 Status Indicators The various indicator lights appearing on the Agilent 218 Purification Solution are color coded to represent the status of the instrument Table3 Status indicator color coding Indicator color Description Green The instrument is in normal standby mode Orange A potential hazard is present Blue
42. user has to assure that under operating conditions the radio interference limits are still met within the premises 74 440 Fraction Collector User Manual Appendix 11 CE Compliance Your Agilent 700 Series ICP OES instrument has been designed to comply with the requirements of the Electromagnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Agilent has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with these directives is indicated by e the CE Marking appearing on the rear of the product and e the documentation package that accompanies the product containing a copy of the Declaration of Conformity The Declaration of Conformity is the legal declaration by Agilent that the product complies with the directives listed above and shows the EN standards to which the product was tested to demonstrate compliance 440 Fraction Collector User Manual 75 11 Appendix Electromagnetic Compatibility 76 EN55011 CISPR11 Group 1 ISM equipment group 1 contains all ISM equipment in which there is intentionally generated and or used conductively coupled radio frequency energy which is necessary for the internal functioning of the equipment itself Class A equipment is equipment suitable for use in all establishments other t
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