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Esco Labculture® Plus Class II Type A2 Biosafety Cabinet

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1. B15 mm 32 1 0 9 meter 3 1 2 meter 4 1 5 meter 5 1 8 meter 6 Testing Opening Height 228 6 mm 9 228 6 mm 9 228 6 mm 9 203 2 mm 8 Working Access Height 274 mm 10 8 274 mm 10 8 274 mm 10 8 218 mm 8 6 Electrical outlet Retrofit Kit provision 10 0 9 meter 3 model one single outlet in workzone 1 2 1 5 and 1 8 meter 4 5 amp 6 models two single outlets in workzone N Flectrical Electronics panel Fluorescent lamp Plugged service fixture provisions 2 on each side Stainless steel single piece work tray 14 Safety glass sliding sash window Stainless steel armrest 15 Single piece stainless steel back wall Drain valve Retrofit Kit provision and side walls _ Esco Sentinel microprocessor contro 16 Removable side panel for plumbing system access Optional Exhaust Collar Positions for Thimble Ducting for LA2 Models 129 mm 5 1 Side View All Sizes 333 mm 13 1 269 mm 10 6 Top View LA2 4A_ Top View LA2 5A_ 691 mm 27 2 Top View LA2 6A_ ESO WORLD CLASS WORLDWIDE General Specifications North America Models LA2 3A2 LA2 4A2 LA2 5A2 LA2 6A2 USA Canada Mexico 115 V 60 Hz Nominal Size 0 9 meters 3 1 2 meters 4 1 5 meters 5 1 8 meters 6 External Dimensions 1115 x 810 x 1540 mm 1420 x 815 x 1640 mm 1725 x 815 x 1540 mm 2030
2. Downflow Supply ULPA Filter First Exhaust ULPA Filter Second Exhaust ULPA Filter 6 Table 5 Esco Airstream Class III Biohazard Safety Cabinet Airflow Profile Air taken in from above the cabinet passes through a pre filter and an ULPA filter Moving into the main chamber of the cabinet in a vertical air stream it provides product protection from airborne particulate matter and cross contamination This vertical flow air stream is also known as the downflow Close to the work surface the downflow air stream splits with a portion entering grilles towards the front or back of the cabinet providing a uniform downflow over the entire working area of the chamber Esco s unique airflow design ensures that air within the cabinet is replaced consistently eliminating excessive air turbulence and the accumulation of particulate contamination in dead air corners Air is moved within the cabinet below the work table where is passes through two ULPA filters one mounted below the work table and the other above the main chamber This provides a fail safe mechanism in case any one of the ULPA filters fails to maintain containment Esco s unique blower system maintains negative pressure within the main work chamber under all circumstances Unlike the competition our Class III biohazard safety cabinets do not utilize supply blowers which when improperly balanced may result in the working area being
3. Airflow Volume 820 Pa 3 29 in H 0 690 Pa 2 77 in H 0 640 Pa 2 57 in H 0 620 Pa 2 49 in H O Pressure Capacity 50 Requirements for Exhaust Ducting 100 Supply ULPA Filter Typical Efficiency Exhaust HEPA Filter Typical Efficiency 1060 Pa 4 26 in H 0 880 Pa 3 53 in H 0 810 Pa 3 25 in H 0 770 Pa 3 09 in H 0 gt 99 999 for particle size between 0 1 to 0 3 microns gt 99 99 at 0 3 microns Sound Emission Fluorescent Lamp Intensity At Zero Ambient Cabinet LB2 4B2 LB2 6B2 gt 124 foot candles gt 134 foot candles gt 112 foot candles NSF ANSI 49 lt 62 5 dBA lt 62 5 dBA lt 64 dBA lt 64 5 dBA EN 12469 lt 59 5 dBA lt 59 5 dBA lt 61 dBA lt 61 5 dBA gt 1335 Lux gt 1440 Lux gt 1200 Lux gt 1230 Lux gt 114 foot candles 1 5 mm 0 06 16 gauge electro galvanized steel with Isocide white oven baked epoxy polyester powder coating Construction All other variants 1 2 mm 0 05 18 gauge electro galvanized steel with Isocide white oven baked epoxy polyester powder coating Electrical Net Weight Shipping Weight Shipping Dimensions Maximum W x D x H Shipping Volume Maximum 220 240V AC 50Hz 10 LB2 3B1 LB2 4B1 LB2 5B1 LB2 6B1 110 130V AC 60Hz 10 LB2 3B2 LB2 4B2 LB2 5B2 LB2 6B2 220 240V AC 60Hz 10 LB2 3B3 LB2 4B3 LB2 5B3 LB2 6B3 288 kg
4. 1 5mmt 0 06 16 gauge electro galvanised steel with white oven baked epoxy anti microbial Isocide SEED tose n h antimicrobial powder coated finish 220 240VAC Cabinet 575W 4A Maximum Power 50Hz 1Ph Outlet 1000VA 5A Consumption Current 110 130VAC Cabinet 765W 11 5A 60Hz 1Ph Outlet 500VA 5A Net Weight Approximate 275 kgs 606 Ibs Max Shipping Dimensions L x W x H 1520 x 950 x 1800 mm 60 x 37 4 x 70 9 Max Shipping Volume 2 6 cbm 92 cbf Your Local Distributor Esco Biotechnology Equipment Division Ecc Q 21 Changi South Street 1 Singapore 486777 Tel 65 6543 0833 Fax 65 6542 6920 Email biotech escoglobal com Website biotech escoglobal com Philadelphia PA USA Houston TX USA Leiden The Netherlands Yokohama Japan Beijing China Shanghai China Kuala Lumpur Malaysia Mumbai India Dubai U A E Esco Labculture Lead Shielded Class Il Type A2 Biosafety Cabinet A Esco Labculture Lead Shielded Class Il with the standard horizontal sliding lead glass The Esco Labculture Lead Shielded Class Il biosafety cabinet is designed for radiopharmaceutical industry usage and protects the operator during work involving radioisotopes SUPERIOR PROTECTION FOR RADIOISOTOPE LABORATORIES The operator is protected from radiation by the lead shielded sides lead shielded work zone bottom and front sliding
5. ULPA Filter Typical Efficiency Downflow Exhaust gt 99 999 at 0 1 to 0 3 microns as per IEST RP CC001 3 USA gt 99 995 at MPPS as per EN 1822 H 14 EU oae kuce NSF ANSI 49 lt 64 dBA lt 65 dBA lt 65 dBA lt 66 dBA Typical EN 12469 lt 61 dBA lt 62 dBA lt 62 dBA lt 63 dBA Fluorescent Light Intensity At Zero Ambient gt 97 foot candles gt 1190 Lux gt 111 foot candles gt 1040 Lux gt 1020 Lux gt 95 foot candles gt 920 Lux gt 85 foot candles Main Body Cabinet 1 2 mm 0 06 16 gauge electrogalvanized steel with white oven baked epoxy Isocide antimicrobial powder coated finish Construction Work Zone 1 5 mm 0 06 16 gauge stainless steel type 304 with 4B finish Side Walls 1 2 mm 0 05 18 gauge stainless steel type 304 220 240V AC 50Hz 1 AC2 3S1 AC2 4S1 AC2 5S1 AC2 6S1 Electrical 110 130V AC 60Hz 1 AC2 3S2 AC2 4S2 AC2 5S2 AC2 6S2 220 240V AC 60Hz 1 AC2 3S3 AC2 4S3 AC2 5S3 AC2 6S3 Net Weight 180 kg 397 Ibs 218 kg 481 Ibs 295 kg 650 Ibs 305 kg 672 lbs Shipping Weight 230 kg 507 Ibs 292 kg 644 Ibs 426 kg 939 lbs 354 kg 780 lbs Shipping Dimensions Maximum W x D x H 1130 x 860 x 1650 mm 44 5 x 33 9 x 65 0 1440 x 860 x 1650 mm 5074 X33905 2100 x 950 x 1880 mm 82 7 x 37 4 x 74 0 1750 x 860 x 1650 mm 68 9 x 33 9 x 65
6. Pending LNE certification to EN12469 Air cleanliness ISO 14644 1 Class 3 IEST G CC1001 IEST G CC1002 and other equivalent requirements Filter performance IEST RP CC034 1 IEST RP CC007 1 IEST RP CC001 3 and EN1822 Electrical safety IEC 61010 1 EN 61010 1 UL 61010A 1 CSA C22 2 No 1010 1 92 Standards Compliance Alios Initial setpoint 0 33 m s or 65 fpm Velocities Downflow or Uniformity is better than 15 Airflow Volumes Inflow 396 cmh 233 cfm At Initial Airflow Downflow 65 772 cmh 454 cfm Velocity Setpoints Exhaust 35 396 cmh 233 cfm ISO14644 1 Class 3 US Federal Standard 209E Class 1 M1 5 AS1386 Class 1 5 JIS B9920 Class 3 BS5295 Class C Class M10 000 as per KS27030 1 and equivalent classes of VDI2083 and AFNOR X44101 ULPA filter with integral metal guards and filter frame gaskets fully compliant with EN 1822 and IEST RP CC001 3 requirements each cabinet has individual downflow and exhaust filters Cleanliness Within Working Area Downflow and Exhaust Filter Type Filter Efficiency Ratings Typical 99 9998 at 0 3um 99 9998 at 0 12um 99 9997 at MPPS lt 56 5 dBA Noise Level The above measurement was taken at initial blower speed setting on a standard 4ft model as per EN12469 specifications Figures are subject to acoustic properties of test environment Light Intensity gt 1200 Lux measured at work surface level zero background as per NSF49 test grid
7. Shipping Volume Maximum 1 6 m 57 cu ft 2 04 m 72 cu ft Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand e Acceptance The number of Bacillus atrophaeus CFU recovered on agar plates shall not exceed 2 CFU per test KI Discus Containment Test According to EN 12469 2000 Potassium lodide Esco is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is defined in the European Standard for microbiological safety cabinets EN12469 2000 as a test method for validating the operator personnel protection capabilities of the cabinet e The KI Discus test shows excellent correlation with the microbiological test method for operator protection 3 75 m 132 cu ft 2 48 m 88 cu ft and is useful for validating the actual containment performance of the cabinet on site e The Kl Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus each Esco Airstream FC2 model is type tested using the KI Discus method for operator safety For Microbiological Safety Cabinets Standards Compliance Type tested to EN 12469 2000 Europe For Air Quality ISO 14644 1 Class 3 Worldwide IEST G CC 1001 USA IEST G CC 1002 USA For Filtration IEST RP CC034 1 Worldwide IEST RP CC007 1 Worldwide IEST RP CC001 3 Wo
8. are tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron par ticles these provide better filtration capability than conventional H13 HEPA filters that have a typical efficiency of gt 99 99 for 0 3 micron particles Color coded indicator lamps display Programmable automatic UV light timer simplifies green for fan operation blue for fluo operation enhances contamination control extends rescent lights and electrical outlet and UV lamp life and saves energy orange for UV lamp ON caution CLASS II BIOSAFETY CABINET Read all safety related instructions before use Operale umil continuously lor bert performance Test certify this cabinet at least annually Dragi ie T OHT Sasia Fre iwa PS S01 Caeelity Certified kana ar tiring Prerana Enlarged touchpad data entry buttons with tactile feedback permit control settings and access to diagnostics default settings and hierarchical menus Mini pleat Separatorless Filter left vs Sentinel Microprocessor Control Conventional Aluminium Separator Filter right Alarm Monitorin g System The Esco Sentinel microprocessor based control system supervises operation of all cabinet functions e Continuous monitoring of cabinet air flow is displayed on a bright easy to read LCD panel The large display monitors operational parameters Esco cabinets use Swedish Camfil Farr mini pleat filters without aluminum separators to increase filter e
9. 0 58 m 6 2 sq ft 0 73 m 7 7 sq ft 0 87 m 9 3 sq ft Average Inflow 0 45 m s 90 fpm at initial setpoint audible visual alarm will activate at 0 40 m s 80 fom Airflow Velocity Downflow 0 30 m s 60 fom at initial setpoint with uniformity of better than 20 Inflow 185 m h 109 cfm 270 m3 h 159 cfm 356 m h 209 cfm 441 m h 260 cfm 527 m h 310 cfm Airflow Downflow Volume 423 m h 249 cfm 563 m h 331 cfm 741 m3 h 436 cfm 919 m h 591 cfm 1096 m h 645 cfm Exhaust 185 m h 109 cfm 270 m3 h 159 cfm 356 m h 209 cfm 441 m h 260 cfm 527 m h 310 cfm ULPA Filter Downflow Typical Efficiency Exhaust gt 99 999 at 0 1 to 0 3 microns as per IEST RP CC001 3 USA gt 99 995 at MPPS as per EN 1822 H 14 EU Sound NSF 49 lt 65 dBA lt 64 dBA lt 65 dBA lt 65 dBA lt 66 dBA Emission Typical EN 12469 lt 62 dBA lt 61 dBA lt 62 dBA lt 62 dBA lt 63 dBA Fluorescent Light Intensity At Zero Ambient gt 900 Lux gt 84 foot candles gt 1130 Lux gt 105 foot candles gt 1280 Lux gt 119 foot candles gt 1050 Lux gt 97 foot candles gt 1220 Lux gt 113 foot candles Main Body Cabinet 1 2 mm 0 06 16 gauge electrogalvanized steel with white oven baked epoxy Isocide antimicrobial powder coated finish Eaa aoa VVork Zone 1
10. ULPA filter with integral metal guards and filter frame gaskets Typical efficiency 99 999 at 0 3um 0 12um and MPPS fully compliant with EN 1822 and IEST RP CC001 3 requirements HEPA filter with integral metal guards and filter frame gaskets Typical efficiency 99 99 at 0 3um fully compliant with EN 1822 and IEST RP CC001 3 requirements Choose from the following options and specify option number when ordering e g AB2 4S1 for 220 240VAC 50HZ 1 220 240VAC 50HZ 1 phase 2 110 130VAC 60HZ 1 phase 3 220 240VAC 60HZ 1 phase 4 110 130VAC 50HZ 1 phase 5 100 110VAC 50HZ 60HZ Cleanliness Within Working Area Downflow Filter Type Exhaust Filter Type Power Supply Options Your local distributor ESCO Esco Biotechnology Equipment Division Esco Biotech is a highly focused manufacturer of laminar flow biohazard safety and other HEPA filtered cabinets for the laboratory with a history of quality cabinets since 1978 We are predominantly oriented towards the international marketplace with sales in more than 70 countries and 95 of turnover exported Our products have been independently tested to stan dards such as AS1807 5 and EN12469 Products are manufactured under 1SQ 001 15SQO 14001 an ISO 9001 registered quality system ee 7222107 21 Changi South Street 1 Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 Email biotech escoglobal com biotech escoglobal com Global Offices Philadelphi
11. 2 Blower 2 on each side 2 amp 3 ft model only 1 single outlet 11 Esco Sentinel microprocessor 3 Downflow ULPA filter 6 Stainless steel work zone in work zone left side wall control system 4 Standard UV light Retrofit Kit 8 Electrical panel 12 Sash window Provision 9 Fluorescent lamp cont Ergonomic foot rest Microbiological Testing Lamp is positioned away from operator line of sight for safety and proper exposure to interior surfaces PVC armrest Chemically treated improves operator comfort easy to clean 712 mm 28 standard size Ergonomic lab chair Laboratory grade construction meets Class 100 cleanliness alcohol resistant PVC materials Adjustable 395 490 mm 15 6 19 3 Angled helps maintain proper posture Adjustable height Anti skid coating chemical resistant finish e V bar with hooks Stainless steel construction Available for all standard Esco cabinets e Microscope viewing device Mounting and viewing pouch integrated into sash Factory installed specify when ordering IS E antimicrobial Powder Coating Bacterial Population 10 fold 0 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours Contact Time in Hours Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and international criteria Testing in our microbiology laboratory is conducted according t
12. 634 Ibs 335 kg 739 Ibs 386 kg 851 Ibs 438 kg 906 Ibs 336 kg 741 Ibs 391 kg 862 Ibs 450 kg 991 Ibs 508 kg 1120 Ibs 1230 x 940 x 1900 mm 49 8 x 35 4 x 73 4 1530 x 940 x 1900 mm 59 1 x 35 4 x 73 4 1910 x 940 x 1900 mm 74 8 x 35 4 x 74 8 2150 x 940 x 1980 mm 82 7 x 35 4 x 74 8 2 20 m 71 3 cu ft 2 73 m 89 2 cu ft 3 41 m 114 7 cu ft 4 00 m 126 8 cu ft These figures are the required exhaust fan pressure capacity left at the cabinet s duct connection plus 50 and 100 extra safety margin but not including the presure capacity required to overcome the pressure drop from bends elbows valves etc in the duct work These required pressure capacity has been claculated to overcome the increased exhaust HEPA filter pressure drop by 50 and 100 due to particle loading The minimum recommended pressure capcity is 50 but to prolong the life of exhaust filte 100 is a safer choice Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Comprehensive Performance Testing At Esco Labculture Biological Safety Cabinet e Class II Type B2 Total Exhaust Biological Safety Cabinets Every Labculture LB2 model manufactured by Esco is individually tested documented by serial number and validated with the following test methods Inflow downflow velocity PAO aerosol cha
13. Light tubes are mounted out of the air stream for better airflow uniformity UV resistant tempered glass window with built in stainless steel glove ports for the number of gloves ports refer to engineering diagrams provided is designed to be 100 leak free for microbiological work with agents assigned to biological safety levels 1 2 3 or 4 Designed for an absolute level of containment they are frequently used for work involving the deadliest biohazards bacteria viruses and microorganisms Manufactured to meet and exceed the latest Class III biohazard safety cabinet requirements of the EN 12469 2000 Industrial grade support frame constructed of electro galvanised steel with an abrasion resistant oven baked powder coated finish Cabinet airflow is regulated by double fans refer to diagrams on page 3 thus ensuring a fail safe system that guarantees absolute safety Permanently lubricated direct drive centrifugal blower s energy efficient external rotor motor type design reduces operating costs Built in solid state variable speed controller infinitely adjustable from zero to the maximum setting with built in RFI and noise filters is superior to conventional step controllers Complete welded construction ensures an air tight cabinet carcass for absolute containment and operator environmental safety Exclusive Dynamic Chamber design surrounds all contaminated air plenums with negative pressure p
14. m Increased diameter liquid chamber inlets to allow the formalin and ammonia solutions to be poured into the chambers more easily without spillage m Internal heat insulation to prevent cross heating of chambers and damage to the electrical and electronics components m Manual shut off isolating switch and safety fuse m Universal IEC power inlet design appropriate cordset must be specified on order depending on country of use Control System Features The first and only microprocessor controlled vaporizer unit in the world specifically designed for safety cabinet decontamination The Esco Formalin Vaporizer FVP OOX is a microprocessor controlled unit that simplifies and automates the decontamination process for all sizes and makes of biohazard safety cabinets This also increases safety for the decontamination operator and laboratory personnel since no manual intervention is required during the entire process The unit enables decontamination to be accomplished by the standard protocol as recommended in EN12469 European standard for microbiological safety cabinets with formalin and ammonia solutions ANSI NSF49 American standard for Class II cabinets recommends decontamination using paraformaldehyde and ammonium bicarbonate which is a similar protocol but with agents in the solid instead of liquid phase and as such this vapouriser can also be said to be in compliance with the ANSI NSF49 recommended decontamination prot
15. plumbing access 17 Integral exhaust collar Accessories and Options Cont e Cradle stand electrical hydraulic infinitely adjustable with casters Elevates to seating or standing work surface height When lowered permits movement through standard doorway Note Increases exterior dimensions Electrical Outlets and Utility Fixtures e Electrical outlet ground fault North America e Electrical outlet Europe Worldwide e Petcock air gas vacuum North America American style Europe Worldwide style DIN 12898 DIN 12919 DIN 3537 Cabinet Accessories e Germicidal UV lamp Controlled by automatic UV lamp timer through Sentinel micro processor control panel Emission of 253 7 nanometers for most efficient decontamination Lamp is positioned away from operator line of sight for safety and proper exposure to interior surfaces Note UV lamp intensity reduces over time and its effectiveness is subject to factors such as relative humidity in the cabinet ambient air temperature and microbial species in the work zone e PVC armrest Chemically treated improves operator comfort easy to clean 712 mm 28 standard size e Ergonomic lab chair Laboratory grade construction meets Class 100 cleanliness alcohol resistant PVC materials Adjustable 395 490 mm 15 6 19 3 Ergonomic foot rest Angled helps maintain proper posture Adjustable height Anti skid coating chemical res
16. positively pressurized e All exhaust air is ULPA filtered and exhausted directly back to the laboratory without re circulation Optionally an exhaust collar can be fitted to allow air to be ducted via a dedicated ducting system to the external environment e Air velocity through an open glove port will be a minimum of 0 7m s 140fpm when a single port is open Class III Biohazard Safety Cabinets are commonly specified for use with the world s deadliest microbes Drawing on the resources and expertise of our cleanroom engineering division Esco is able to provide a complete turn key service for the construction of critical environments to complement applications in which Class III cabinets may be used Although a Class III safety cabinet provides an unparalleled degree of operator and environmental protection safety can still be increased by the construction of negative pressure laboratories and the use of isolation suits When a Class III safety cabinet is operated within a negative pressure laboratory any failure in the Class III cabinet will still be isolated within the laboratory Assuming the cabinet fails contamination from the cabinet will spill over into the laboratory but will not contaminate the general environment since all air within the laboratory is filtered before being exhausted to the external environment Engineering Diagrams for Available Sizes GLOVE SIZES To find out your glove size
17. prevent damage to the filter media 10 The filters shall be a individually scan tested by 11 12 the manufacturer b individually scan tested after assembly and b easily accessible for scan testing in situ by means of a dedicated upstream sampling port accessible from within the cabinet The supply filter shall be angled and oriented to the 10 cabinet front angle to maximize downflow uniformity over the work surface A removable perforated metal diffuser shall be installed below the supply filter to optimize airflow uniformity and to protect from damage Blower System 13 14 15 16 17 The cabinet shall have a direct drive permanently lubricated centrifugal blower motor dynamically balanced in two planes compliant to ISO 2710 for low noise low vibration and long filter life The blower motor shall have an external rotor design and include an automatic thermal cut out to disable the motor in case of overheating The blower motor shall have an automatic speed control to compensate for filter loading The blower motor system shall be enclosed within a dynamic chamber shaped steel plenum and integrated with the removable supply filter assembly to simplify filter changing The cabinet shall be equipped with an integral exhaust collar for connection to the laboratory ducting system Cabinet Design Construction Cleaning 18 19 20 21 22 23 The cabinet shall be of trip
18. 0 15 0 20 0 25 0 30 Particle Size um 99 999 typical efficiency for particle sizes of 0 1 to 0 3 micron level ESO WORLD CLASS WORLDWIDE 0 40 0 50 ULPA filtered air L Unfiltered potentially contaminated air _ Room air Inflow air Blower Efficiency The Labculture blower system is designed for high performance operation maximum energy efficiency and minimal maintenance e The blower motor can maintain airflow velocity within 10 of original setpoint for a 50 increase in pressure loss over clean filters without manual speed control adjustment e The permanently lubricated direct drive external rotor motor blower reduces operating costs e The external rotor motor design allows for optimum cooling of the motor during extended operations and extends the motor bearing life Side capture zones Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the front grille to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum blower plenum at the top of the cabinet Ambient air is taken in through a pre filter at the top of the cabinet and passes through the downflow ULPA filter entering the work zone as laminar flow The uniform non turbulent air stream protects against cross contamination wit
19. 3 kgs 1163 lbs Max Shipping Weight 601 kgs 1325 Ibs 751 2 kgs 1656 Ibs 901 5 kgs 1988 Ibs Max Shipping Dimensions 1995 x 1300 x 2750 mm WxDxH 78 5 x 51 2 x 108 3 2245 x 1300 x 2750 mm 2550 x 1300 x 2750 mm 88 4 x 51 2 x 108 3 100 4 x 51 2 x 108 3 Max Shipping Volume 7 13 cbm 251 8 cbf 8 03 cbm 283 6 cbf 9 12 cbm 322 07 cbf ECCO Esco Biotechnology Equipment Division Esco Biotech is a highly focused manufacturer of laminar flow biohazard safety and other HEPA filtered cabinets for the laboratory with a history of quality cabinets since 1978 We are predominantly oriented towards the international marketplace with sales in more than 70 countries and 90 of turnover exported Our products have been independently tested to stan dards such as AS1807 5 and EN12469 Products are manufactured under an ISO 9001 registered quality system A ene local distributor BEL TE HE IT HERA BEC Tree HHPA a J 21 Changi South Street 1 Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 Email biotech escoglobal com biotech escoglobal com ESCO Formalin Vaporizer for Decontamination Decontamination Cycle m Operator starts process by pressing a button on the touch pad m Pre delay timer allows decontamination operator to seal up the cabinet before vaporization of ammonia commences Delay period can be set from 1 to 59 minutes m Vaporization of formalin solution commen
20. 6 x 25 6 72 8 x 25 6 x 25 6 2 ports refer to drawing on page 3 4 ports refer to drawing on page 3 4 ports refer to drawing on page 3 Neoprene polychloroprene synthetic rubber gloves Available in sizes 7 8 standard size and 9 Air Volume At Initial Velocity 603 cmh 355 cfm 756 cmh 445 cfm 902 cmh 531 cfm Cabinet negative pressure 274Pa 1 1inH O exceeds EN12469 2000 requirement of 199 3Pa 0 8inH O and NSF49 requirement of 124 5Pa 0 5inH2O Individually performance tested and certified at factory under controlled conditions for General requirements IEST RP CC002 2 and AS1386 5 Standards Compliance Air cleanliness ISO 14644 1 Class 3 IEST G CC1001 IEST G CC1002 and other equivalent air cleanliness requirements Filter performance IEST RP CC034 1 IEST RP CC007 1 IEST RP CC001 3 and EN1822 Electrical safety IEC 61010 1 EN 61010 1 UL 61010A 1 CSA C22 2 No 1010 1 92 Air Cleanliness Within ISO 14644 1 Class 3 US Federal Standard 209E Class 1 M1 5 AS 1386 Class 1 5 JIS B9920 Class 3 BS5295 Class C Working Area Class M10 000 as per KS 27030 1 and other equivalent cleanliness classifications of the VDI 2083 and AFNOR X44101 Main Filter Downflow Type Exhaust x2 Downflow Main Filter Efficiency Ratings 1st Exhaust 2nd Exhaust ULPA filter with integral metal guards and filter frame gaskets fully compliant with EN1822 and IEST RP CC001 3 requirements ULPA filters with
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22. Front Sash Assembly and Sash Seal The magnetic switch eliminates the chance of mechanical wear and tear typical of a mechanical switch The laminated glass maintains containment if the sash is accidentally broken during cabinet operation The back of the sash can be easily cleaned by removing the sash track cover to swing up the sash glass ESO WORLD CLASS WORLDWIDE Operator Product and Environmental Protection Infinity Class Il Model FC2 4A_ delivers three times better downflow uniformity than required by European Standard EN 12469 and provides protection from airborne con taminants to the operator product and the environment Model FC2 4A_ shown with optional height adjustable stand with casters and optional adjustable footrest Advanced Engineering The Esco Infinity microbiological safety cabinet includes a number of design and performance features not found on our popular Labculture series cabinets These include e An aerosol tight window for additional safety while the cabinet is inoperative e Double fan design guarantees safety in the event of the failure of one fan e Motorized front sash for one hand operation e Larger LCD display for easy monitoring of operational parameters e Reduced height permits cabinet instal lation for seated operation in standard 2 4 meter nominal lt 8 laboratory ceil ing height Cabinet shown on optional mounting stand with casters seated height w
23. Latin America 230 V 50 amp 60 Hz LA2 3J5 LA2 4J5 LA2 5J5 LA2 6J5 Nominal Size 0 9 meters 3 1 2 meters 4 1 5 meters 5 1 8 meters 6 External Dimensions 1115x810 x 1540 mm 1420 x 815 x 1540 mm 1725 x 815 x 1540 mm 2030 x 815 x 1540 mm WxDxH 43 9 x 31 9 x 60 6 55S x 32 1 XOS 67 9 x 32 1 x 60 6 79 9 x 32 1 x 60 6 x Gross Internal Dimensions 955 x 623 x670 mm 1260 x 623 x 670 mm 1565 x 623 x 670 mm 1870 x 623 x 670 mm WxDxH 37 200 X 2A 5E pos 49 6 x 24 5 x 26 4 61 6 x24 5 x 26 4 73 6 x24 5 x 26 4 Usable Work Area 0 45 m 4 8 sq ft 0 6 m 6 5 sq ft 0 75 m 8 1 sq ft 0 9 m 9 7 sq ft Tested Opening 228 6 mm 9 228 6 mm 9 228 6 mm 9 203 2 mm 8 Working Opening 274 mm 10 8 274 mm 10 8 274 mm 10 8 248 mm 9 8 INC Ee Nim low 53 m s 105 pin Velocity Downflow 0 35 m s 70 fpm 0 35 m s 70 fpm 0 35 m s 70 fpm 0 33 m s 65 fpm Inflow 419 m h 246 cfm 552 m h 325 cfm 685 m h 403 cfm 742 m h 437 cfm Downflow 63 702 m h 413 cfm 924 m h 544 cfm 1147 m h 675 cfm 1289 m h 758 cfm Exhaust 37 419 m h 246 cfm 552 m h 325 cfm 685 m h 403 cfm 742 m h 437 cfm Airflow Volume Required Exhaust ee pore 707 m h 416 cfm 901 m h 530 cfm 1114 m h 656 cfm 1256 m h 739 cfm Thimble Exhaust Collar ULPA Filter Typical Eff
24. Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet work zone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B atrophaeus spores is placed inside the work zone 10 cm 4 behind the front opening sash e Target slit air samplers and impingers are placed outside the work zone to capture possibly escaping B atrophaeus spores then the sample is incubated e Acceptance The number of Bacillus atrophaeus CFU recovered from the agar plates shall not exceed 10 CFU per test Product Protection Test The test objective is to determine cabinet protection to the product samples inside the cabinet work zone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B atrophaeus is placed at 10 cm 4 in front of sash window 2 48 m 88 cu ft 2 98 m 105 cu ft e Target agar plates are placed through out the entire work surface e Acceptance The number of Bacillus atrophaeus CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet protection from cross contamination of samples placed simultaneously inside the work zone e Anebulizer containing 55 mL of spores 5 to 8 x 10 mL is placed against one of the work zone sidewalls e Target agar plates are placed 360 mm 14 away from th
25. The control panel is located on the center efficiency minimize the chance of leakage and to of the cabinet and angled down for easy prolong filter life Filters include a lightweight alumi num frame for structural stability and elimination of access by the operator swelling common to conventional wood frames e A back up battery maintains alarm function for airflow power and pressure s Filter assembly is constructed in accor alarm in the event of a power failure dance with EN1822 requirements e Two integrated temperature com pensated true airflow velocity sensors provide independent measurement of inflow and downflow velocities despite room temperature fluctuation e The supply filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection e Modern separatorless mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction e The exhaust filter traps biohazard par ticles acquired from the work surface before air is exhausted to the room offering personal and environmental protection Input Voltage e The exhaust filter media is protected Esco Next Generation Sentinel Microprocessor Control System The Sentinel microprocessor based control and alarm system sup
26. blower motor light UV lamp electrical outlet s and menu The controller shall be user programmable in situ to enable or disable functions such as PIN personal identification number access restriction cabinet start up protocol airflow alarm and other microprocessor controlled operations outlined in the user manual When programmed ON the start up protocol shall perform an automatic pre purge and post purge cycle to ensure proper cabinet operation The controller shall include a blower motor hours meter to display aggregate motor running time to assist in predictive maintenance Audible and visual alarms shall be provided for unsafe conditions such as improper airflow or sash position Airflow shall be monitored by a temperature compensating thermistor based true air velocity sensor mounted in the cabinet The airflow display and alarm system shall be individually calibrated before shipment The main control panel shall exhibit continuous display of air velocity and a 24 hour clock display Certification Service and Decontamination 44 The cabinet shall be approved for both hydrogen peroxide vapor HPV and formaldehyde decontamination protocol 45 All panels leading to potentially contaminated and 46 47 or hazardous areas shall be color coded red All components with the exception of blower motor and ULPA filters shall be located outside of contaminated air spaces to facilitate servicing withou
27. cabinets tempered glass sides include multi piece tray components which lift and remove to provide easy access and to encourage surface decontamination e A recessed central area and drain pan channels spills and prevent liquids from entering the lower filtration and blower systems e The drain pan is flush with the side walls to eliminate concealed or hard to clean spaces e There are no screws on the front or sides to trap contaminants or complicate cleaning e External surfaces are coated with Esco Isocide antimicrobial coating to protect against surface contamination and inhibit bacterial growth Isocide eliminates 99 9 of surface bacteria within 24 hours of exposure Service Fitting Access The cabinet is prepared for easy installation of optional gas and vacuum fittings see Accessories Biological Safety Cabinet e Class II Microbiological Safety Cabinets E Series S Series and D Series Touchpad data entry buttons permit control settings and access to diagnostics default settings and hierarchical menus Programmable automatic UV light timer simplifies operation enhances contamination control extends UV lamp life and saves energy Color coded indicator lamps display green for primary function fan operation blue for secondary function fluorescent lights and electrical outlet and orange for caution UV lamp ON Sentinel Microprocessor Control System Programmable e Optional service fitt
28. configured at shutdown ensuring all residue contaminants are purged out of the workzone before deactivation Class II Type B2 biohazard safety cabinets provide product operator and environmental protection and are suitable for general microbiological work with agents assigned to biological safety levels I II or III In a Class II Type B2 cabinet all inflow and downflow air is exhausted after HEPA ULPA filtration to the external environment without m Fail safe system ensures that in case of exhaust failure the cabinet s main fan automatically shuts down to ensure safety to the user and the environment m An admin PIN can be set to restrict unauthorized access to all menu functions ERGONOMIC FEATURES m Attractive stainless steel internal side walls and back wall are made from a single sheet of stainless steel which eliminates welded joints where contaminants may accummulate The single piece inner liner enhances the cleanability of the workzone and is non glaring for operator comfort m Additional UV interlock ensures UV lamp feature is deactivated when the sash is not fully closed m Built in 5000k fluorescent lighting offers excellent illumination throughout the workzone Electronically ballasted lighting system is instant start non flickering and energy efficient m Sloped front design and frameless front sliding sash allow for maximum visibility into the workzone m Cabinet armrest is raised above the work
29. e Class II Microbiological Safety Cabinets S Series E Series and Airstream Duo Improved lighting is brighter more uniform reduces glare m Optional UV lamp is located behind control panel away from line of sight lamp operates on programmable timer Airstream Duo Class II Biological Safety Cabinet with stainless steel side dual blower motors and dual exhaust filter Model AC2 4D_ Containment and Protection e A combination of a supply ULPA filter and an exhaust ULPA filter create a fully integrated performance envelope for product operator and environmental protection e Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination e Raised armrest prevents the likelihood of inflow grille blocking by operator s arms e Auto purge holes located at the front side walls on AC2 S Series eliminate eddy currents and dead air pockets in the critical area behind the sash window e The inflow velocity downtlow velocity air flow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front aperture this curtain maintains personal and product protection even in the unlikely event of a severe inflow or downflow imbalance that would compromise protection in a conventional cabinet Integrated Filtration System Independent supply and exhaust filters provide 99 999 typical efficien
30. for handling Biosafety Level 4 provided that the operator wears a positive pressure sult particle sizes of 0 1 to 0 3 microns Airstream filters meet the IEST RP CC001 3 recommended practice for ULPA performance USA and EN 1822 for H14 performance EU Dual Blower Motors mum containment ULPA filters per IEST RP CC001 3 are tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron particles these provide better filtration capability than conventional H13 HEPA filters that have a typical efficiency of gt 99 99 for 0 3 micron particles Modern separatorless mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction The filter assembly is constructed in accordance with EN1822 requirements The supply filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection The exhaust filter traps biohazard particles acquired from the work surface before air is exhausted to the room offering personal and environmental protection The Airstream Duo includes two exhaust filters to double exhaust protection The exhaust filter media is protected from mechanical damage by an integrated metal screen guard which is absent from conventional HEPA filters ESCO WORLD CLASS WORLDWID
31. for structural stability and elimination of swelling common to conventional wood frames Esco ULPA Filter Efficiency Typical Penetration e Modern separator less mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction e The filter frame and media is constructed in accordance with EN1822 requirements for fire retardant properties e The supply ULPA filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection e The exhaust ULPA filter traps biohazard particles acquired from the work surface before air is exhausted to the room offering personnel and environmental protection e The exhaust filter media is protected from mechanical damage by an integrated metal screen guard which is absent from conventional HEPA filters e The top cabinet surface is slanted to discourage users from placing objects which could block the exhaust filter and reduce inflow velocity Front Sash Assembly e Integrated sash proximity contacts sense proper sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned e The magnetic switch eliminates the chance of wear and tear typical of a mechanical switch e The back of the sash can be easily cleaned b
32. integral metal guards and filter frame gaskets fully compliant with EN1822 and IEST RP CC001 3 requirements Minimum 99 997 at 0 3um 99 988 at 0 12um 99 987 at MPPS Typical 99 99927 at 0 3m 99 9977 at 0 12um 99 9972 at MPPS Minimum 99 99962 at 0 3m 99 99965 at 0 12um 99 99946 at MPPS Typical 99 999928 at 0 3m 99 99996 at 012um 99 999924 at MPPS Minimum 99 99916 at 0 3m 99 9985 at 0 12um 99 9982 at MPPS Typical 99 99983 at 0 3m 99 99979 at 012um 99 99971 at MPPS Pre Filter Disposable and non washable polyester fibers with 85 arrestance EU3 rated Noise Level Typically lt 60 dBA at initial blower speed setting measured as per EN 12469 2000 based on 4 feet cabinet subject to acoustic properties of test environment Light Intensity gt 1700 lux gt 158 foot candles measured at work surface level zero background as per IEST RP CC002 2 Main Body Construction 1 5mmt 0 06 16 gauge electro galvanised steel with white oven baked epoxy powder coated finish Front Window Construction Colourless and transparent UV absorbing 8mm 0 31 tempered glass Work Surface Construction 1 2mmt 0 05 18 gauge stainless steel grade 304 Maximum 220 240VAG Power 50Hz 1Ph 1144W 4 97A 1164W 5 06A 1180W 5 13A Consumption 110 130VAC Current 60Hz 4Ph 1632W 13 6A 1652W 13 77A 1668W 13 9A Net Weight Approximate 351 5 kgs 775 lbs 439 4 kgs 969 Ibs 527
33. leveling feet With casters e Adjustable height hydraulic range 711 mm to 864 mm 28 to 34 With leveling feet With casters e Telescoping height stand for leveling feet nominal range 660 mm or 960 mm 26 or 37 8 e Telescoping height stand for casters nominal range 660 mm or 880 mm 26 or 34 6 Adjustable in 25 4 mm 1 increments All exterior painted surfaces are powder coated with Esco Isocide an antimicrobial inhibitor to minimize contamination Isocide is integrated into the coating substrate and cannot wash out or diminish by repeated cleaning Performance results are available upon request Contact Esco or your Esco Sales Representative for details Model LA2 Biological Safety Cabinet Technical Specifications 955 mm 1260 mm 1565 mm 1870 mm 1540 mm 60 6 Exterior height for 110 130 V 60 Hz models of 1 2 m and 1 8 m 4 and 6 is 1640 mm 64 6 37 6 49 6 61 6 73 6 1115 mm 1420 mm 1725 mm 2030 mm 43 9 7 599 67 9 79S 1 Exhaust ULPA filter Blower Downtlow ULPA filter angled UV light Retrofit Kit provision for 0 9 1 2 and 1 5 meter 3 4 amp 5 models UV light Retrofit Kit provision for 1 8 meter 6 models 4a 4b IV Bar Retrofit Kit provision Cabinet Size models of 1 2 m and1 8 m 4 and 6 is 715 mm 28 1 Internal height for 110 130V 60Hz
34. measure in inches around your hand with a tape measure at the place indicated on the diagram This measurement in inches is the closest to your glove size i e 8 is equal to a glove size of 8 You should use your dominant hand the right if you are right handed and the left if you are left handed AC3 4 Standard Model AC3 5 External Width 1 34m 4ft External Width 1 645m 5ft 1340 mm 52 8 1645 mm 64 8 1665 mm 65 6 1970 mm 77 6 AC3 6 AC3 Side Diagram for All Sizes External Width 1 95m 6ft 650 mm 25 6 2250 mm 88 6 450mm 17 7 400 mm 15 7 450 mm 17 7 900 mm 35 4 1950 mm 76 8 2275 mm 89 6 Engineering Details 1 Esco Sentinel Microprocessor Control 2 Pass Box 3 Pre filter 4 Electrical Electronics Panel 5 ULPA Filter Downflow 6 Fluorescent Light 7 Glove Ports Gloves available in sizes 7 8 amp 9 8 First Exhaust ULPA Filter 9 First Exhaust Blower 10 Second Exhaust ULPA Filter 11 Second Exhaust Blower 12 Optional UV Lamps 13 Pressure Gauge 14 Work Zone Back Wall Optional Electrical Outlet Retrofit Kit Provisions 2 outlets in work zone one on each side 15 Optional Exhaust Collar Esco Catalogue Revision No AC3 v3 6 2005 Specifications subject to change without notice Technical Specifications 2005 Esco Micro Pte
35. mm 10 7 430 mm 16 9 666 mm 26 2 820 rm 32 3 LB2 3 LB2 4 LB2 5 LB2 6 Sr Nas RS Sasa 50 mm 2 0 250 mm 9 84 250 mm 9 84 1568 mm 61 7 _ 955 mm 1260 mm 1565 mm 1870 mm 37 6 749 6 61 6 73 6 1115 mm 1420 mm 1725 mm 2030 mm 43 9 7 56 0 67 9 780 0 Cabinet Size 0 9 meter 3 1 2 meter 4 1 5 meter 5 1 8 meter 6 Blower Downflow ULPA filter angled UV light Retrofit Kit provision QQ N 0 9 meter 3 model one single outlet in workzone 1 2 1 5 and 1 8 meter 4 5 amp 6 models two single outlets in workzone IV Bar Retrofit Kit provision 7 Electrical Electronics panel 8 Fluorescent lamp Testing Opening Height 203 mm 8 203 mm 8 203 mm 8 203 mm 8 Working Access Height 248 mm 9 8 248 mm 9 8 248 mm 9 8 248 mm 9 8 Exhaust UI PA filter 6 Electrical outlet Retrofit Kit provision 9 Plugged service fixture provisions 2 on each side 11 Stainless steel armrest control system 815 mm 32 1 10 Stainless steel single piece work tray 12 Drain valve Retrofit Kit provision 13 Esco Sentinel microprocessor 14 Safety glass sliding sash window 15 Single piece stainless steel back wall and side walls 16 Removable side panel for
36. personnel in the contagious area the community and the environment from exposure to potentially infectious aerosols A Class I or Class II biohazard safety cabinet is required for work involving these agents Biosafety Level 4 Practices safety equipment and facilities appropriate for work done with dangerous and exotic agents which pose a high risk of life threatening disease May be transmitted via the aerosol route and for which there is no available vaccine or therapy Members of the laboratory staff have specific and thorough training in handling extremely hazardous infectious agents and they understand the primary and secondary containment functions of the standard and special practices the containment equipment and the laboratory design characteristics They are supervised by competent scientists who are trained and experienced in working with these agents Access to the laboratory is strictly controlled by the laboratory director The facility is either in a separate building or in a controlled area within a building which is completely isolated from all other areas of the building A specific facility operations manual is prepared or adopted A Class III biohazard safety cabinet is required for work involving these agents From Biosafety in Microbiology and Biomedical Laboratories U S Department of Health and Human Services HHS publication CDC 99 8395 4th ed April 1999 p 11 14 lane Ad A Guide To Biosafety And Biosafety
37. positive pressure plenums capable of handling contaminated air shall be surrounded by negative pressure No positive pressure areas shall be accessible external to the cabinet The third wall shall conceal utilities 19 The cabinet shall maintain containment performance even when removable work area components are removed for cleaning 20 The work tray shall be one piece removable Stainless steel with radius corners without crevices or joints 21 The cabinet shall have a stainless steel one piece fabricated drain trough with open angles to channel spills to a common drain 22 The closed sidewall shall be sealed without perforations return air slots or concealed areas which can contain contaminants 23 The cabinet shall be free of sharp edges non functional protrusions bolts screws or hardware and all metal edges shall be deburred 24 The cabinet exterior top shall be slanted to discourage placement of foreign objects and to maintain proper exhaust airflow Ergonomics and Convenience 25 The front sash shall be frameless to maximize visibility and accessible for cleaning front and back Sash glass shall be safety glass 26 The sash counterbalance shall be suspended on two high strength cables and the sash shall lock into position in the event one cable becomes detached 27 Magnetic not mechanical proximity sensors shall work in conjunction with the control system to indicate proper sash position for co
38. provision 8 Electrical outlet Retrofit Kit provision 10 Fluorescent lamp 2 Blower 6 Plugged service fixture provisions 2 amp 3 ft model only 1 single outlet 11 Esco Sentinel microprocessor gt Daun ULPA Titer 2 on each side in work zone left side wall control system 4 Standard UV light Retrofit Kit 7 Stainless steel work zone 9 Electrical panel 12 Tempered glass sash window Provision Airstream Biological Safety Cabinet e Class II Microbiological Safety Cabinets E Series S Series and D Series General Specifications Airstream Class II Biological Safety Cabinets E Series Note to customer Insert electrical voltage number into last model number digits _ when ordering Model Nominal Size External Dimensions WxDxH Internal Work Area Dimensions WxDxH Internal Work Area Space AC2 2E_ AC2 3E_ AC2 4E_ AC2 5E_ 0 6 meters 2 730x 740 x 1435 mm 28 7 x29 1 x 56 5 0 9 meters 3 1035 x 40 x 1360 mm 40 7 x 29 1 x 52 9 1 2 meters 4 1340 x 740 x 1360 mm 52 7 x 29 1 x 52 9 1 5 meters 5 1645 x 740 x 1360 mm 64 7 x 29 1 x 52 9 AC2 6E__ 1 8 meters 6 1950 x 740 x 1360 mm 76 7 x29 1 x 529 660 x 560 x 670 mm 26 0 x22 0 x 26 4 965 x 560 x 6 0 mm 38 0 x22 0 x 26 4 1270 x 560 x 670 mm 50 0 x 22 0 x 26 4 1575 x 560 x 670 mm 62 0 x22 0 x26 4 1880 x 560 x 670 mm 74 0 x22 0 x 26 4 0 29 m 3 1 sq ft 0 43 m 4 6 sq ft
39. sash Horizontal sliding lead glass piece provides additional protection to the operator during work involving radioisotopes The air enters the cabinet through the front air grille and travels through the Kl impregnated carbon filter on which radioactive iodine is adsorbed Air then travels to the blower plenum where it is filtered by high efficiency ULPA filters before being exhausted ESCO www escoglobal com li Main Features Class II Product Operator Environment Protection Extra Safety Cabinet air is filtered via 2 exhaust filters This feature allows for filter changing without prior decontamination of the cabinet Lead Shielded Protection 3mm lead shielding on cabinet sides work zone bottom Radiation proof glass with lead equivalence of 3mm Horizontal sliding lead glass piece for additional operator protection KI potassium iodide impregnated carbon filter for iodine adsorption ULPA Filters with typical efficiency at gt 99 999 at 0 12um provide ISO Class 3 cleanliness within work zone Isocide antimicrobial coated structure eliminates 99 9 of bacterial presence on external surfaces within 24 hours Ergonomic sloped front eliminates glare off the front window and ensures operator comfort during extended usage Microprocessor based control and alarm system Extremely low noise level for quiet working environment Additional options Lead shielded back panel Increa
40. should not be used in BSCs that recirculate exhaust air to the room i e Class I that are not ducted to building exhaust systems or Class IIA1 or Class IIA2 cabinets Class II B1 cabinets are acceptable for work with minute amounts of volatile chemicals and radionuclides A Class IIB2 cabinet also called a total exhaust cabinet is necessary when significant amounts of radionuclides and volatile chemicals are expected to be used 11 A Guide To Biosafety And Biosafety Cabinets B Using Biological Safety Cabinets in The Laboratory 1 Location The velocity of air flowing through the front opening into a BSC is about 0 45 m s At this velocity the integrity of the directional air inflow is fragile and can be easily disrupted by air currents generated by people walking close to the BSC open windows air supply registers and opening and shutting doors Ideally BSCs should be situated in a location remote from traffic and potentially disturbing air currents Whenever possible a 30 cm clearance should be provided behind and on each side of the cabinet to allow easy access for maintenance A clearance of 30 35 cm above the cabinet may be required to provide for accurate air velocity measurement across the exhaust filter and for exhaust filter changes 2 Operators If BSCs are not used properly their protective benefits may be greatly diminished Operators need to be careful to maintain the integrity of the front opening air inflow when mo
41. status and alarm functions Color coded indicator lamps display green k for primary function fan operation blue for secondary function fluorescent lights and electrical outlet and orange for caution UV lamp ON All functions can be user activated through touch pad programming access see Operations Manual Read all satety related instructions beatore usa Test cortity this cabinet at least annually ESCO When programmed ON e the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities Lat Tred fa line IE Ls BTT Sm Faby 1 ra ma Provieciian Stina weir reer e The airflow alarm can be activated or deactivated depending on user preference and nature of the work Consult your Esco Operating Manual or contact your Sales Representative for information on user preference programming capabilities built into the Sentinel microprocessor platform Cabinet Construction Robust construction and enhanced safety features qualify the cabinet for the most demanding laboratory applications The cabinet is fully assembled and ready to install The interior work area is formed from a single piece of stainless steel with large radius corners to simplify cleaning The cabinet work zone has no welded joints to collect contaminants or rust All stainless
42. steel work surfaces are accessible for cleaning Tray components lift and remove to provide easy access and encourage surface decontamination A recessed central area and stainless steel drain pan channels spills and prevent liquids from entering the lower walls to eliminate concealed or hard to clean spaces e There are no screws in on the front or sides to trap contaminants or complicate cleaning e Optional service fittings are offset for easier access e External plumbing is concealed behind trim panels to maintain aesthetics e External surfaces are coated with Esco lsocide antimicrobial coating to protect against surface contamination and inhibit bacterial growth Isocide eliminates 99 9 of surface bacteria and operate when shipped filtration and blower systems e The drain pan is flush with the side within 24 hours of exposure Robust Cabinet Construction and Enhanced Safety Features Service fixtures are offset for easier reach Standard cabinets include two fixture provisions on each sidewall one provision on each sidewall for 0 9 meter 3 ft cabinet Electrical outlets are mounted below service fixtures to minimize obstructions Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated gas spring struts providing front service access All key components with the exception of the blower motor assembly are mounted outside the air stream and away from contamin
43. the operator None of the unfiltered inflow air enters the work zone of the cabinet so the product inside the work zone is not contaminated by the outside air A feature unique to Class II cabinets is a vertical laminar unidirectional HEPA filtered air stream that descends downward from the interior of the cabinet This continuously flushes the cabinet interior of airborne contaminants and protects samples being handled within the cabinet from contamination and is Known as the downflow The differences between the various Class II cabinets available lie primarily with the percentage of air exhausted to that of air re circulated from the common air plenum In addition different Class II cabinets have different means of cabinet exhaust Some cabinets may exhaust air directly back to the laboratory while others may exhaust air through a dedicated ductwork system to the external environment Despite these differences all Class II cabinets like Class I cabinets protect both the operator and environment from exposure to biohazards In addition Class II cabinets also protect product samples from contamination during microbiological manipulations within the cabinet interior and are all suitable for work with agents assigned to biological safety levels 1 2 and 3 A Guide To Biosafety And Biosafety Cabinets Class II Type A A1 A2 Biohazard Safety Cabinet The Class II Type A biohazard safety cabinet is the most common Class II cabinet It i
44. workzone 10 cm 4 inches behind the front opening sash e Target slit air samplers and impingers are placed outside the workzone to capture The Performance Envelope Concept 0 65 m s High Inflow 0 60 High Downflow 0 61 0 43 High Inflow Low Downflow 0 58 0 27 0 55 ominal 0 53 0 35 0 50 Inflow Velocity 0 45 0 35 Low Inflow Low Downflow 0 325 0 25 0 20 0 25 0 30 Downflow Velocity Labculture Low Inflow High Downflow 0 32 0 45 0 35 0 40 0 45 possibly escaping B atrophaeus spores then the sample is incubated e Acceptance The number of Bacillus atrophaeus CFU recovered from the agar plates shall not exceed 10 CFU per test Product Protection Test The test objective is to determine cabinet protection to the product samples inside the cabinet workzone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B atrophaeus is placed at 10 cm 4 inches in front of sash window e Target agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus atrophaeus CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet protection from cross contamination of samples placed simultaneously inside the workzone e A nebulizer containing 55 mL of spores 5 to 8 x 10 mL is placed against one of
45. x 815 x 1640 mm WxDxH 43 9 x 31 9 x 60 6 55 9 x 32 1 x 64 6 67 9 x 32 1 x 60 6 79 9 x 32 1 x 64 6 Gross Internal Dimensions 955 x 623 x 6 0 mm 1260 x 623 x 715 mm 1565 x 623 x 670 mm 1870 x 623 x 715mm WxDxH 37 6 x 24 5 x 26 4 AO O X 2AL5 x 72ers 61 6 x 24 5 x 26 4 TIO X25 XLII Usable Work Area 0 45 m 4 8 sq ft 0 6 m 6 5 sq ft 0 75 m 8 1 sq ft 0 9 m 9 7 sq ft Tested Opening 228 6 mm 9 228 6 mm 9 228 6 mm 9 203 2 mm 8 Working Opening 274 mm 10 8 274 mm 10 8 274 mm 10 8 248 mm 9 8 Average Airflow BALON 53 m s 105 fpm Velocity Downflow 0 35 m s 70 fpm 0 35 m s 70 fpm 0 35 m s 70 fom 0 33 m s 65 fom Inflow 419 m3 h 246 cfm 552 m h 325 cfm 685 m h 403 cfm 742 m3 h 437 cfm Downflow 63 702 m h 413 cfm 924 m h 544 cfm 1147 m h 675 cfm 1289 m3 h 758 cfm Exhaust 37 419 m h 246 cfm 552 m h 325 cfm 685 m h 403 cfm 742 m h 437 cfm Airflow Volume Required Exhaust Wih Optional 707 m h 416 cfm 901 m h 530 cfm 1114 m h 656 cfm 1256 m h 739 cfm Thimble Exhaust Collar ULPA Filter Typical Efficiency gt 99 999 for particle size between 0 1 to 0 3 microns per IEST RP CC001 3 NSF ANSI 49 lt 66 dBA lt 64 5 dBA lt 66 dBA lt 67 dBA Sound Emission EN 12469 lt 63 dBA lt 61 5 dBA lt 63 dBA lt 64 dBA rss EEA sns gt 1230 Lux
46. 12469 Potassium lodide Esco is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is defined in the European Standard for microbiological safety cabinets EN 12469 as a test method for validating the operator personnel protection capabilities of the cabinet e The KI Discus test shows excellent correlation with the microbiological test method for operator protection and is useful for validating the actual containment performance of the cabinet on site e The KI Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus each Esco Labculture LA2 model is factory tested using the KI Discus method for operator safety Purchase Specifications LA2 Series Class Il Type A2 Biological Safety Cabinet General Performance and Certifications 1 The biological safety cabinet shall comply with one or more of the following international standards and the manufacturer shall provide a certified copy of containment and performance tests equivalent to or greater than specified in the following independent international standards for biological safety electrical and other functional characteristics Class Il Type A2 per NSF ANSI 49 USA Class Il per EN 12469 JIS K3800 SFDA YY 0569 SANS12469 2 The cabinet shall protect a the operator and laboratory environment from particulates generated within the work zone b the prod
47. 159 cmh 94 cfm ISO 14644 1 Class 3 US Federal Standard 209E Class 1 M1 5 AS 1386 Class 1 5 JIS B9920 Class 3 BS5295 Class C Class M10 000 as per KS 27030 1 and equivalent classes of VDI 2083 and AFNOR X44101 Downflow and Exhaust Filter Type ULPA filter with integral metal guards and filter frame gaskets fully compliant with EN 1822 and IEST RP CC001 3 requirements each cabinet has individual downflow and exhaust filters Filter Efficiency Ratings Minimum 99 9995 at 0 3um 99 9994 at 0 12um 99 9991 at MPPS Typical 99 9999 at 0 3um 99 9999 at 0 12um 99 9998 at MPPS Noise Level Typically lt 60 dBA at initial blower speed setting according to EN 12469 2000 subject to acoustic properties of test environment Light Intensity gt 750 Lux gt 70 foot candles measured at work surface level zero background as per NSF49 test grid Main Body Construction 1 5mmt 0 06 16 gauge electro galvanized steel with white oven baked epoxy powder coated finish Side Wall Construction Work Surface Construction Colourless and transparent UV absorbing 5mm 0 2 tempered glass 1 2 mmt 0 05 18 gauge stainless steel grade 304 with BA finish Maximum 220 240VAC Cabinet Power Current 300W 1 5A Power SOHz1Ph Outlet Power Current 1000VA 5A Consumption 110 130VAC Cabinet Power Current 385W 3 5A Current 60Hz 1Ph Outlet Power Current 500V
48. 2469 e The sliding window can be fully opened to insert and remove larger instrumen tation and equipment Electrical Safety and Certification All components meet or exceed applicable safety requirements e Each cabinet is individually factory tested for electrical safety e Documentation specific to each cabinet serial number is maintained on file e Certified to the renowned world stan dard for microbiological safety cabinets the TUV GS mark for compliance to EN 12469 May 2007 e Contact Esco or your Sales Representative for site preparation information see Electrical Specifications Warranty The Infinity is warranted for 3 years exclud ing consumable parts and accessories e Each cabinet is shipped with a compre hensive User s Manual complete with a report documenting all test procedures e Additional IQ OQ PQ documentation is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests ESO WORLD CLASS WORLDWIDE Microbiological Safety Cabinets Standards Compliance Certified TUV GS to EN 12469 2000 May 2007 Air Quality ISO 14644 1 Class 3 Worldwide AS 1386 Class 1 5 Australia JIS B9920 Class 3 Japan BS 5295 Class C UK KS27030 1 Class M10 000 Korea IEST G CC 1001 USA IEST G CC 1002 USA Models FC2 4A1 and FC2 6A7 Certification for additional models is pending or in process For details on mo
49. 29 or 838 mm 33 38 1 mm 1 5 With leveling feet With casters e Adjustable height hydraulic range 737 mm 29 to 838 mm 33 D With casters e Telescoping height stand nominal range 737 mm 29 or 838 mm 33 Adjustable in 25 4 mm 1 increments e Infinitely adjustable cradle stand with casters Elevates to seating or standing work surface height When lowered permits movement through standard doorway Note Increases exterior dimensions Electrical Outlets and Utility Fixtures e Electrical outlet ground fault North America e Electrical outlet Europe Worldwide e Petcock air gas vacuum North America American style Europe Worldwide style DIN 12898 DIN 12919 DIN 3537 Cabinet Accessories e Germicidal UV lamp Controlled by automatic UV lamp timer through Sentinel micro processor control panel Emission of 253 7 nanometers for most efficient decontamination ESO WORLD CLASS WORLDWIDE Cabinet Filtration System Stainless Steel Side Walls Tempered Side capture zones applicable for AC2 S Series Glass only Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air p
50. 35 mm 56 0 x 31 7 x 60 4 FC2 5A_ 1 5 meters 5 1725 x 805 x 1535 mm 67 9 x 31 7 x 60 4 FC2 6A_ 1 8 meters 6 2030 x 805 x 1535 mm 80 0 x 31 7 x 60 4 955 x 610 x 670 mm 37 6 x 24 0 x 26 4 Internal Work Area Dimensions WxDxH 1260 x 610 x 670 mm 49 6 x 24 0 x 26 4 1565 x 610 x 670 mm 61 6 x 24 0 x 26 4 1870 x 610 x 670 mm 73 6 x 24 0 x 26 4 Internal Work Space Area 0 47 m 5 06 sq ft 0 62 m 6 67 sq ft 0 77 m 8 29 sq ft 0 92 m 9 90 sq ft Tested Opening 200 mm 7 9 200 mm 7 9 200 mm 7 9 200 mm 7 9 Working Opening 210 mm 8 3 210 mm 8 3 210 mm 8 3 210 mm 8 3 Power Rating Code 1 0 48 m s 95 fpm at initial setpoint Average Airflow rules 2 0 53m s 105 fpm at initial setpoint Velocity Downflow 25 0 35m s 70 fpm at initial setpoint 1 3 332 m h 195 cfm 437 m h 257 cfm 593 m h 319 cfm 646 m h 380 cfm Inflow 2 336 m h 216 cfm 483 m3 h 284 cfm 599 m h 353 cfm 714 m h 420 cfm Airflow Volume Downflow 1 2 3 702 m h 413 cfm 929 m h 544 cfm 1147 m h 675 cfm 1367 m h 804 cfm 1 3 332 m h 195 cfm 437 m h 257 cfm 543 m h 319 cfm 646 m h 380 cfm Exhaust 2 336 m h 216 cfm 483 m h 284 cfm 599 m h 353 cfm 714 m h 420 cfm 12 lt 60 dBA lt 61 dBA lt 63 d
51. 4 1 USA Electrical Safety UL C 61010A 1 USA CSA22 2 No 1010 192 Canada EN 61010 1 Europe IEC61010 1 Worldwide Warranty The Labculture cabinet is warranted for 3 years excluding consumable parts and accessories e Each cabinet is shipped with a comprehensive user s manual complete with a report documenting all test procedures e Additional IQ OQ PQ documentation Is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests Accessories and Options Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Represen tative for ordering information Support Stands e Fixed height available 711 mm 28 or 864 mm 34 38 1 mm 1 5 With leveling feet With casters e Telescoping height stand for leveling feet nominal range 660 mm or 960 mm 26 or 37 8 e Telescoping height stand for casters nominal range 660 mm or 880 mm 26 or 34 6 Adjustable in 25 4 mm 1 increments All exterior painted surfaces are powder coated with Esco Isocide an antimicrobial inhibitor to minimize contamination Isocide is integrated into the coating substrate and cannot wash out or diminish by repeated cleaning Performance results are available upon request Contact Esco or your Esco Sales Representative for details Model LB2 Biological Safety Cabinet Technical Specifications 271
52. 5 mm 0 06 16 gauge stainless steel type 304 with 4B finish Side Walls UV absorbing tempered glass 5 mm 0 2 colorless and transparent 220 240V AC 50Hz 1 AC2 2E1 AC2 3E1 AC2 4E1 AC2 5E1 AC2 6E1 Electrical 110 130V AC 60Hz 12 AC2 2E2 AC2 3E2 AC2 4E2 AC2 5E2 AC2 6E2 220 240V AC 60Hz 12 AG2 2E3 AC2 3E3 AC2 4E3 AC2 5E3 AC2 6E3 Net Weight 160 kg 353 Ibs 177 kg 390 Ibs 277 kg 611 lbs 270 kg 595 Ibs 300 kg 661 Ibs Shipping Weight 187 kg 412 Ibs 230 kg 507 lbs 322 kg 710 Ibs 326 kg 718 lbs 354 kg 780 Ibs Shipping Dimensions Maximum W x D x H 850 x 860 x 1750 mm 33 5 x 33 9 x 68 9 1140 x 890 x 1715 mm 44 9 x35 x67 5 56 7 x 33 9 x 65 1440 x 860 x 1650 mm 1750 x 860 x 1650 mm 68 9 x 33 9 x65 2060 x 860 x 1680 mm 81 1 x 33 9 x 66 1 Shipping Volume Maximum 1 28 m 45 6 cu ft 1 6 m 57 cu ft 2 04 m 72 cu ft Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand for 48 hours and the Colony Forming Units CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone Bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel
53. A 5A Net Weight Approximate 160 kgs 352 Ibs Max Shipping Weight 240 kgs 530 Ibs Max Shipping Dimensions L x W x H 885 x 917 x 1690 mm 34 8 x 36 1 x 66 5 Max Shipping Volume 1 37 cbm 48 4 cbf ECCO Esco Biotechnology Equipment Division Esco Biotech is a highly focused manufacturer of laminar flow biohazard safety and other HEPA filtered cabinets for the laboratory with a history of quality cabinets since 1978 We are predominantly oriented towards the international marketplace with sales in more than 70 countries and 90 of turnover exported Our products have been independently tested to stan dards such as AS1807 5 and EN12469 Products are manufactured under an ISO 9001 registered quality system 21 Changi South Street 1 Singapore 486 777 Tel 65 6542 0833 Fax Your local distributor ISO 9001 50 14001 REGISTERED FIRM REGISTERED FIRM 65 6542 6920 Email biotech escoglobal com biotech escoglobal com Labculture O SHIA ES NSF 49 UL 61010 JIS K3800 SFDA YY 0569 EN 12469 SANS 12469 Labculture Class Il Type A2 N Biosafety Cabinet Model LA2 4A_ Class Il Type A2 Biological Safety Cabinets The Safety Solution for Life Science Laboratories Labculture g 3 O gt A Main Features Unique Esco Dynamic Chamber plenum m Negative pressure plenum surrounds contaminated positive pressure plenum no fabric bags a
54. A Guide To Biosafety amp Biosafety Cabinets A Guide To Biosafety And Biosafety Cabinets Foreword This booklet was developed as a guide to biosafety cabinets that provides basic knowledge of biosafety and biosafety cabinets The information presented is unbiased and generic in nature compiled with help from experienced microbiologists engineers and safety enclosure users I Biohazards and Biosafety Levels The word biohazard is a contraction of the words biological and hazard and defined as an infectious agent or part thereof presenting a real or potential risk to the well being of man animals and or plants directly through infection or indirectly through disruption of the environment Biosafety Levels 1 through 4 were established by the Centers for Disease Control CDC and the National Institutes of Health NIH and are combinations of laboratory practices and techniques safety equipment and facilities All of these levels are appropriate for the biohazard posed by the agents used and for the laboratory activity Biosafety Level 1 Practices safety equipment and facilities appropriate for work with defined and characterized strains of viable micro organisms not known to cause disease in healthy adult humans The laboratory is not necessarily separated from the general traffic patterns in the building Work is generally conducted on open bench tops using standard microbiological practices Special containment equipme
55. BA lt 64 dBA NSF 49 ie 2 lt 61 dBA lt 62 dBA lt 64 dBA lt 65 dBA Sound Emission Typical Te lt 57 dBA lt 58 dBA lt 60 dBA lt 61 dBA EN 12469 2 lt 58 dBA lt 59 dBA lt 61 dBA lt 62 dBA Downflow ULPA Filter Typical Efficiency Exhaust gt 99 995 at MPPS as per EN 1822 H 14 EU gt 99 999 at 0 1 to 0 3 microns as per IEST RP CC001 3 USA Fluorescent Lamp Intensity gt 1200 Lux 111 foot candles Main Body Isocide antimicrobial powder coated finish 1 2 mm 0 06 16 gauge electrogalvanized steel with white oven baked epoxy Cabinet Construction Work Zone Stainless steel grade 304 Work tray 1 2 mm 0 04 Walls and drain pan 0 9 mm 0 03 Side Walls Stainless steel grade 304 220 240V AC 50Hz 1 Al FC2 3A1 FC2 4A1 FC2 5A1 FC2 6A1 Electrical 110 130V AC 60Hz 1 A2 FC2 3A2 FC2 4A2 FC2 5A2 FC2 6A2 220 240V AC 60Hz 12 FC2 3A3 FC2 4A3 FC2 5A3 FC2 6A3 Net Weight 228 kg 503 Ibs 298 kg 657 Ibs 372 kg 820 Ibs 447 kg 985 Ibs Shipping Weight 280 kg 617 lbs 354 kg 780 Ibs 443 kg 977 lbs 522 kg 1150 Ibs 1200 x 900 x 1870 mm 47 2 AIDA X730 Shipping Dimensions Maximum W x D x H 1530 x 900 x 1870 mm 60 2 x 35 4 x 73 0 1910 x 900 x 1870 mm 75 2 XISSA X730 2150 x 900 x 1870 mm 84 6 x 35 4 x 73 0 Shipping Volume Maximum 2 02 m 71 cu ft Additional vol
56. E Mini pleat Separatorless Filter left vs Conventional Aluminum Separator Filter right Esco cabinets use Swedish Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the chance of leakage and to prolong filter life Filters include a lightweight aluminum frame for structural stability and elimination of swelling common to conventional wood frames Single Blower System Airstream S Series and E Series Models The AC2 blower system is designed for high performance operation maximum energy efficiency and minimal maintenance e Industry exclusive backward curved motorized impeller design replaces conventional blowers e Improved energy efficiency lowers operating costs e Reduced noise and vibration levels over conventional blowers provide a comfortable working environment e An integral blower hour meter tracks operating life and aids in predictive maintenance planning e Built in RFI and electrical noise filters eliminate interference with adjacent instrumentation e The external rotor motor design allows for optimum cooling of the motor during extended operations and extends the motor bearing life e Speed can be adjusted electronically without the need for mechanical adjustment e To prevent fan damage a paper catch grille traps papers or towels that may Esco ULPA Filter Efficiency Typical Penetration drop down on the drain pan preventing the
57. H pue YyI eaH Jo JuUawedIag S N saliojzesoge7z jedsipawoig pue ABbojoiqossip ul Ajasesolg 5 inos QA2T Ajajesolg A Guide To Biosafety And Biosafety Cabinets II Biological Safety Cabinets The terms biological safety cabinet and biosafety cabinet have been widely used to describe a variety of containment devices equipped with HEPA filter s designed to provide personnel or both personnel and product protection from biohazardous materials The terms should only be applied to those devices that meet the requirements of Class I II or III specifications based on their construction airflow velocities and patterns and their exhaust systems Major International Standards for Biosafety Cabinets American Standard NSF 49 2002 European Standard EN 12469 2000 Australian Standard AS 2252 Japanese Standard JIS K 3800 2000 Table 2 Biohazard Safety Cabinet Classes Recycle Exhaust Control Plenum Exhaust Biosafety Air Air Surrounded by Alternatives Level US 0 38 Inside room 1 2 amp 3 I 0 100 Outside Air ai 70 30 Quisidersife 7 ie 2 70 30 Negative Plenum side room f 1 283 TE ooo 30 70 Negative Plenum ee lp DEG io 5 0 100 Negative Plenum ae 1 2 amp 3 1 sae 0 100 Negative Plenum sige rooms 2384 Source Laboratory Biosafety Manual Second Edition revised Interim guidelines World Health Organization p32 A Guide To Biosafety And Biosafety Cabinets Class I Biohazard Safety Cabi
58. Ltd Custom made Cabinets Esco Airstream Class III biosafety cabinets are available in full stainless steel on a customized basis suitable for the pharmaceutical industry Contact Esco for more information Left Custom made 4ft fully stainless steel Esco Airstream Class III cabinet AC3 4SX AC3 4BX Size General Specifications Optional Retrofit Kits and Accessories The following optional Retrofit Kits are available from Esco for Airstream Class III biohazard safety cabinets Esco s Retrofit Kit system allows the user to field upgrade their cabinets with optional accessories post purchase NOTE Service fixtures are not allowed in Class III biosafety cabinets as Class III biosafety cabinet construction is required to be airtight Germicidal UV lamp Retrofit Kit Standard electrical socket outlet Retrofit Kits total 6A rating for all outlets in the work zone GFCI ground fault circuit interrupter electrical socket outlets 115VAC units only Hard air tight duct exhaust collar Formalin vaporiser see image For biosafety cabinet decontamination AC3 5BX AC3 6BX 1340 x 850 x 2250 mm 1645 x 850 x 2250 mm 1950 x 850 x 2250 mm Width x Depth x Height 52 8 x 33 5 x 88 6 64 8 x 33 5 x 88 6 76 8 x 33 5 x 88 6 1240 x 650 x 650 mm 1545 x 650 x 650 mm 1850 x 650 x 650 mm Width x Depth x Height 48 8 x 25 6 x 25 6 60 8 x 25
59. The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet work zone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B atrophaeus spores is placed inside the work zone 10 cm 4 behind the front opening sash e Target slit air samplers and impingers are placed outside the work zone to capture possibly escaping B atrophaeus spores then the sample is incubated e Acceptance The number of Bacillus atro phaeus CFU recovered from the agar plates shall not exceed 10 CFU per test Product Protection Test The test objective is to determine cabinet pro tection to the product samples inside the cabinet work zone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B atrophaeus is placed at 10 cm 4 in front of sash window e Target agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus atro phaeus CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet pro tection from cross contamination of samples placed simultaneously inside the work zone e Anebulizer containing 55 mL of 5 to 8 x 104 spores mL is placed against one of the work zone sidewalls e Target agar plates are placed 360 mm 14 away from the same side wall e Acceptance The number of Bacillus atro phaeus CFU re
60. a USA Leiden Netherlands Santiago Chile Shanghai China Kuala Lumpur Malaysia Mumbai India Dubai U A E PT Esco Bintan Indonesia nie a ST a ECO Airstream Class III Biosafety Cabinet MAIN FILTRATION AGENTS ISO Class 3 air cleanliness within work zone as per ISO 14644 1 equivalent to Class 1 as per US Federal Standard 209E 100 times cleaner than the usual Class 100 classification on cabinets offered by the competition All materials used in our products are cleanroom compatible Mini pleat separatorless ULPA filter technology reduces energy consumption and delivers increased laminar airflow uniformity for better product and cross contamination protection ULPA filters are double scan tested at the time of manufacturing and after installation Exclusive vertical laminar flow design ensures that air within the chamber is decontaminated in a consistent fashion thus preventing the accumulation of particulate contamination in dead air corners Product protection is guaranteed by the ULPA filtered vertical laminar air flow within the cabinet workzone Exhaust air is double filtered through high quality ULPA filters with a rated efficiency of 99 999 at 0 12 microns thus ensuring complete product operator and environmental protection from airborne biological hazards a built in pre filter also extends main filter life CONTROL FEATURES Microprocessor based Esco Sentinel co
61. afety Cabinet e Class Il Type A2 Biological Safety Cabinets b Labcultu re Operator Product and Environmental Protection The Esco Labculture Class Il Type A2 Biosafety Cabinet provides operator product and environmental protection against Biosafety Levels 1 2 and 3 This cabinet can be used for handling Biohazard Level 4 provided that the operator wears positive pressure sult Containment and Protection The airflow ratio of 63 recirculation to 37 exhaust increases operator protection beyond the 70 30 ratio of conventional biological safety cabinets e The inflow and downflow balance is precisely established by an external exhaust damper and is adjustable without decontaminating the cabinet e Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination e The front grille has proportionally larger perforations on the extreme left and right side Combined with the side air gap between the work tray and the side wall the stronger side capture zones increase protection in this critical area where contaminants tend to bleed out in conventional cabinets e The convex inflow grille maintains Operator protection by discouraging users from placing objects on it e Auto purge holes located at the front side walls eliminate eddy currents and dead air pockets in the critical area behind the sash window Per NSF ANSI 49 requir
62. ainment Approximately 35 of the air in the common plenum is exhausted through the ULPA filter to the room The remaining 65 of the air is passed through the downflow ULPA filter and into the work area as a vertical laminar flow air stream bathing the work surface in clean air m The uniform non turbulent air stream protects against cross contamination within and throughout the work area Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone Air returns to the common air plenum where the 35 exhaust and 65 recirculation process is continued Airstream Duo Model AC2 D Series Biological Safety Cabinet Technical Specifications Stainless Steel Side Walls Dual Blowers 1475 mm 58 1400 mm 55 1 000 0 00 0 0 965 1270 1575 1880 mm 40 7 52 7 64 7 76 7 732 mm 28 8 1035 1340 1645 1950 mm i 40 7 52 7 64 7 76 7 560 mm 22 7 Exhaust ULPA filter 5 IV bar Retrofit Kit
63. as flow in the event of a cabinet alarm or unsafe condition Comfortable Ergonomic Design The cabinet is engineered for comfort utility value and safety e The angled viewing window and narrow profile front grille improves reach into the work area e The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy e The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Specifications e The front armrest is raised above the work zone to improve comfort and to minimize blockage of forward airflow perforations e The optional adjustable support stand provides work surface height control e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equip ment see Specifications tt t m I y Al f SSeS eS eS SSS 5 cs ws eS SS OS ON SO OS I ES SI ES OS I OS I OS OI SOS NS IS I IS IES IEE ES Inflow Fail 1C If the exhaust blower fails 1c the supply blower continues to provide inflow to the cabinet and downflow to the work area The control panel warns of inflow failure With the exhaust blower offline the cabinet maintains protection by maintaining inflow above 0 40 m s as required by EN 1
64. at MPPS and 99 999 for 0 1 to 0 3 microns 9 An integral filter guard shall be affixed to prevent damage to the filter media 10 The filters shall be a individually scan tested by the manufacturer b individually scan tested after assembly and b easily accessible for scan testing in situ by means of a dedicated upstream sampling port accessible from within the cabinet 11 The supply filter shall be angled and oriented to the 10 cabinet front angle to maximize downflow uniformity over the work surface 12 A removable perforated metal diffuser shall be installed below the supply filter to optimize airflow uniformity and to protect from damage Blower System 13 The cabinet shall have a direct drive permanently lubricated centrifugal blower motor dynamically balanced in two planes compliant to ISO2710 for low noise low vibration and long filter life 14 The blower motor shall have an external rotor design and include an automatic thermal cut out to disable the motor in case of overheating 15 The blower motor shall have an automatic speed control to compensate for filter loading 16 The blower motor system shall be enclosed within a dynamic chamber shaped steel plenum and integrated with the removable supply filter assembly to simplify filter changing 17 The integral damper shall be externally adjustable Cabinet Design Construction Cleaning 18 The cabinet shall be of triple wall design whereby all
65. ated air to permit service without decontamination These include fluorescent lamps UV lamp electrical harnesses electronic boards and microprocessor control Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color coded red to warn service technician The telescoping Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when required Work area containment is maintained even when removable components are lifted out for cleaning The one piece stainless steel work tray edges are radiused and easy to clean without crevices or joints The lower drain trough is a single piece fabrication with wide open angles and a channel to direct spills to the drain The closed sidewall contains no perforations air return slots or other hidden areas where contaminants can accumulate WORLD CLASS WORLDWIDE Biosafety Cabinets Air Quality Filtration Electrical Safety NSF ANSI 49 USA EN 12469 Europe JIS K 3800 Japan SFDA YY 0569 China ISO 14644 1 Class 3 Worldwide JIS B9920 Class 3 Japan JIS BS5295 Class 3 Japan US Fed Std 209E Class 1 USA EN 1822 H14 Europe IEST RP CC001 3 USA IEST RP CC007 USA IEST RP CC034 1 USA UL C 61010A 1 USA CSA22 2 No 1010 192 Canada EN 61010 1 Europe IEC61010 1 Worldwide Standards Compliance Please refer to the specifications table on page 8 for the model listing Dyna
66. ation Selecting the correct type of BSC installing it using it properly and annually certifying its operation are complex processes It is highly recommended that they proceed under the supervision of a well trained and experienced biosafety professional The professional should be highly familiar with the relevant literature listed in the References section and should have been trained on all aspects of BSCs Operators should receive formal training in the operation and use of BSCs A Guide To Biosafety And Biosafety Cabinets C Safe Use of Biosafety Cabinets 1 Using BSCs Startup Wear gloves for hand protection Load the cabinet with all items required prior to starting work and surface decontaminate Surface decontaminate the work surface side walls and inner back walls Allow the work zone air to purge for a few minutes before commencing work Do not overcrowd the work zone Close the drain valve before operation gt Using BSCs Operation Do not obstruct the front or back air grilles Work as far in to the cabinet as possible Minimize arm movement make slow movements to avoid disrupting cabinet airflow When removing arms from cabinet be sure to surface decontaminate first and move arms out slowly of the cabinet in direction perpendicular to plane of work zone opening Minimize external airflow disturbances Work from clean to dirty Biohazard collection bags should be placed inside the
67. boratory Equipment Cytostatic Workbenches Requirements Testing EN12469 Performance Criteria for Microbiological Safety Cabinets Air cleanliness ISO 14644 1 Class 3 IEST G CC1001 IEST G CC1002 and other equivalent requirements Filter performance IEST RP CC034 1 IEST RP CC007 1 IEST RP CC001 3 and EN1822 Electrical safety IEC 61010 1 EN 61010 1 UL 61010A 1 CSA C22 2 No 1010 1 92 Standards Compliance Average Airflow Inflow Initial setpoint 0 45 m s or 90 fpm audible visual alarm will activate at 0 40 m s or 80fpm Velocities Downflow Initial setpoint range 0 30 m s or 60 fpm Airflow Volumes Inflow 460 cmh 270 cfm 680 cmh 400 cfm eae Downflow 65 780 910 cmh 460 535 cfm 1160 1350 cmh 680 794 cfm Airflow Velocity Setpoints Exhaust 35 460 cmh 270 cfm 680 cmh 400 cfm ISO14644 1 Class 3 US Federal Standard 209E Class 1 M1 5 AS1386 Class 1 5 JIS B9920 Class Cleanliness Within Working Area 3 BS5295 Class C Class M10 000 as per KS27030 1 and equivalent classes of VDI2083 and AFNOR X44101 Exhaust Carbon Filter KI potassium iodide impregnated carbon filter installed directly below the work surface ULPA filter with integral metal guards and filter frame gaskets fully compliant with EN 1822 and IEST RP com ia eles CC001 3 requirements Typical efficiency 99 9997 at MPPS 99 9998 at 0 3 and 0 12um Exhaust ULPA Filter Type ULPA filter with inte
68. cabinet instead of outside Use absorbent pads on the work surface where appropriate to minimize splatter and aerosol generation in case of a spillage Surface decontaminate before removing potentially contaminated items from the interior Place aerosol generating instruments as far in to the interior of the cabinet as possible Clean materials should be at least 150mm away from aerosol generating objects to minimize the chance for cross contamination Hold lids covers above dishes sample plates in order to prevent impingement of downward air Do not use a gas flame whenever possible as it interferes with airflow Put air turbulence generating equipment such as centrifuge blender or sonicator in the back 1 3 of the cabinet N 3 Bunsen Burner Burners can cause an outflow of air from inside the cabinet that can endanger the operator On Class II cabinets protection against cross contamination may also be compromised There have been incidents where the sash of a recirculating Class II safety cabinet was closed while the burner was still on causing heat build up in the cabinet that damaged the filters Bunsen Burner Safety 1 An electronic burner which automatically reduces the gas supply to maintain a smaller flame during periods of non use 2 An emergency shut off valve should be easily accessible near to the cabinet in case of an emergency A Guide To Biosafety And Biosafety Cabinets 3 The burner sho
69. ces to release the formaldehyde gas to decontaminate the cabinet m LCD display will show reminder for operator to cycle the cabinet fans to ensure even distribution of the gas m Heater automatically cuts off when formalin solution has completely boiled off Contact time timer starts to regulate the period for which the formaldehyde gas is exposed to the cabinet internal surfaces for the decontamination to take effect m Neutralisation process begins automatically to vaporize the ammonia solution to neutralise the formaldehyde gas m LCD display will show reminder for operator to cycle the cabinet fans to ensure even distribution of the gas m Neutralisation timer starts to regulate the period for which the neutralisation reaction takes place General Construction Features Durable heavy duty full stainless steel construction m Electrical and electronic components are isolated from the heating chamber to prevent exposure to chemical fumes and heat which can reduce component life m Side mounted handles for easy carrying m Generously sized formalin and ammonia chambers 800mL maximum capacity in total which allows even the largest of safety cabinets up to 6 foot console units to be decontaminated with the maximum agent volume recommended in EN12469 Thermostats forthe formalin and ammonia chambers to prevent overheating m Backup thermostat to ensure the temperature of the external casing does not rise above a safe level
70. comfort and to ensure that the operator s arms do not block the forward airflow perforations e The optional adjustable support stand provides work surface height control e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equipment See Specifications e The sliding window can be fully opened to insert and remove larger instrumenta tion and equipment Electrical Safety and Certification All components meet or exceed applicable safety requirements e Each cabinet is individually factory tested for electrical safety e Documentation specific to each cabinet serial number is maintained on file e UL Listed for USA and Canada e Certified to the US standard NSF ANSI 49 for Class Il biohazard cabinetry refer to Standard Compliance table at the top of this page for the full list e Contact Esco or your Sales Representative for site preparation information see Electrical Specifications lt SiD E Antimicrobial Powder Coating Bacterial Population 10 folds 0 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours Contact Time in Hours Labculture Biological Safety Cabinet e Class Il Type B2 Total Exhaust Biological Safety Cabinets Filtration EN 1822 H14 amp H13 Europe IEST RP CC001 3 USA IESI RP CC007 USA IEST RP CC03
71. covered on agar plates shall not exceed 2 CFU per test HPV Test Compliant Safer Hydrogen Peroxide Decontamination Compatibility Esco biological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decon taminatable cabinets approved by BIOQUELL for this patented process HPV is a safer and more efficient alternative to conventional decontamination using formaldehyde CH 0 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has pungent smell e If there is a gap on the cabinet sealing escaping HPV to the lab will decompose to become oxygen and water Escaping form aldehyde however is harmful to people in the lab Therefore HPV decontamination can be performed while people are work ing inside the lab while formaldehyde decontamination must be performed with no one present in the lab The HPV method improves safety productivity and reduces the time to seal the cabinet e HPV biological efficacy is independent of environmental variables whereas form aldehyde efficacy is dependent on such variables e HPV has a better penetration capacity resulting in a full decontamination of the cabinet The formaldehyde method is known to result in incomplete decontamination e HPV is more effective and rapid against bio logical organisms compared to formaldehyde e HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hour
72. cquired from the work surface before air is exhausted via the ducting system to the external environment The Model LB2 is provided with an integral exhaust collar for connecting the cabinet exhaust to an airtight ducting system NB Ductworks are not provided with standard product Front Sash Assembly e Integrated sash proximity contacts sense sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned e The magnetic switch eliminates the chance of mechanical wear and tear typical of a mechanical switch e The back of the sash can be easily cleaned by removing the sash track cover and swing up the sash glass e The sash is counterbalanced for smooth and light sash moving operation e The counterbalance locking mechanism is inherently safe it locks the counter balance in place if either of the 2 cables is detached The sash cable and cable clip have rated strength of more than 6x the weight of the sash window of the largest Labculture Class II cabinet 1 8 meter 6 ft model e The safety glass maintains containment if the sash is accidentally broken during cabinet operation Fsco cabinets use ULPA filters per IEST RP CC001 3 instead of conventional HEPA filters commonly found in biological safety cabinets While HEPA filters offer 99 99 typical efficiency at 0 3 micron level ULPA filters provide 0 0 05 0 10
73. ction and prevents cross contamination of samples Exhaust air is usually HEPA filtered and incinerated Alternatively double HEPA filtration with two filters in series may be utilized Materials are transferred into the cabinet using a pass through unit installed at the side of the work area Class III cabinets usually exhaust air back to the laboratory however air may also be exhausted via a dedicated ductwork system to the external environment When a dedicated ductwork system is employed they are also suitable for work employing toxic chemicals as an adjunct to microbiological processes All Class III biohazard safety cabinets are suitable for work with microbiological agents assigned to biological safety levels 1 2 3 and 4 They are frequently specified for work involving the most lethal biological hazards Fig 4 Class III BSC A Guide To Biosafety And Biosafety Cabinets HEPA amp ULPA Filter The HEPA filter is the heart of the biosafety cabinet The HEPA filter is a disposable dry type filter constructed of boron silicate microfibers cast into a thin sheet much like a piece of paper HEPA High Efficiency Particulate Air Modern American convention HEPA 99 99 at 0 3 microns ULPA Ultra Low Penetration Air Modern American convention ULPA 99 999 at 0 12 microns HEPA ULPA filter construction Fig 6 Separator Type Fig 7 Separatorless Type HEPA ULPA filters are designed to remove a broad rang
74. cy for La _ m Esco ISSZiE antimicrobial surface on all painted surfaces minimizes contamination Integrated RFI and electrical noise filters eliminate interference with and from adjacent equipment E Series with Tempered Glass Side Walls e Multi piece work surface removal simplifies cleaning e Glass sides increase visibility and prevent the operator from being boxed in S Series with Stainless Steel Side Models e Enhanced side capture zones and negative pressure side walls optimize containment e Interior work area formed from a single piece of stainless steel with large radius corners to simplify cleaning D Series Duo with Stainless Steel Sides Dual Exhaust Filters e Dual tan design for redundant operation Dual exhaust filters for added safety If one fan fails the second fan provides mini P Operator Product and Environmental Protection Esco Airstream Class Il Biological Safety Cabinets offer a premium level of operator product and environmental protection with advanced technology at an economical price Intelligent ergonomic design enhances productivity operator comfort maintenance and utility value With an extensive track record of safety reliability and performance Airstream cabinets make ideal investments for a wide range of general laboratory applications Airstream biological safety cabinets provide protection against Biosafety Levels 1 2 and 3 and can be used
75. d operate when shipped e The interior sides and back wall are formed from a single piece of stainless steel with large radius corners to sim plify interior cleaning e The cabinet work zone has no welded joints to collect contaminants or rust e All stainless steel work surfaces are accessible for cleaning e Multi piece tray components are easily lifted and removed to encourage sur face decontamination e A recessed central area and stainless steel drain pan channels spills and prevent liquids from entering the lower filtration and blower systems Double Blower System Provides the maximum possible level of safety by enabling safe cabinet shut down in the event of a single blower failure Ta pa mi 44 nT SSeS eS eS eS eS eS CS eS SS ES IES SES OS IS IES I ES I SI OS IES SOS IES ES SSIES EI ES SES SSE SES EIES 1A Under normal operation with both blowers operating 1a the supply blower creates a negative pressure surrounding the contaminated positive pressure plenum and pushes air across the supply and exhaust filters The exhaust blower boosts the air pressure through the exhaust filter to create better inflow and operator protection Supply and exhaust blowers automatically operating at reduced speeds extend blower life Dynamic Chamber Plenum Design I N
76. d vibration Airflow pattern visualization Electrical safety to IEC61010 1 KI Discus containment Additional microbiological testing is performed on statistical sampling basis ESCO WORLD CLASS WORLDWIDE Labculture Labculture SFI OO Labculture Class Il Type B2 N Biosafety Cabinet Model LB2 4B_ Class Il Type B2 Total Exhaust Biological Safety Cabinets The Safety Solution for Life Science Laboratories Main Features Unique Esco Dynamic Chamber plenum Negative pressure plenum surrounds supply positive pressure plenum no fabric bags are used Long life supply ULPA filter per IEST RP CC001 3 with gt 99 999 efficiency for particle size between 0 1 to 0 3 microns m Angled supply filter matches cabinet profile to achieve best downflow uniformity Esco Sentinel microprocessor Frameless shatterproof sash is easier to clean Ergonomically angled front improves reach and comfort Actual work access height is 45 mm 1 8 higher than tested sash opening to provide additional work space Removable one piece work surface simplifies cleaning Raised airflow grille maintains safety by preventing blockage Optional UV lamp is located behind control panel away from line of sight Does not apply to 1 8 m 6 models A a ral Esco ISS LiDE antimicrobial coating on all painted surfaces minimizes contamination Enhanced side capture zones optim
77. dominantly oriented towards the international marketplace with sales in more than 60 countries and 90 of turnover exported Our products have been independently tested to stan dards such as AS1807 5 and EN12469 Products are manufactured under an ISO 9001 registered quality system Ch SE FS Po BL Pe ELE BrP ee ee Your local distributor 21 Changi South Street 1 Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 Email biotech escoglobal com biotech escoglobal com
78. e capture zones Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum blower plenum at the top of the cabinet Approximately 35 of the air in the common plenum is exhausted through the ULPA filter to the room The remaining 65 of the air is passed through the downflow ULPA filter and into the work area as a vertical laminar flow air stream bathing the work surface in clean air The uniform non turbulent air stream protects against cross contamination within and throughout the work area e The blower motor can maintain airflow for a 150 increase in pressure loss over clean filters without manual speed control adjustment or 210 increase in pressure drop across the filter with manual speed control adjustment Applies to 1 2 meter 4 ft model contact Esco for information on other models e The permanently lubricated direct drive external rotor motor blower reduces operating costs e Built in RFI and electrical noise filters eliminate interference with adjacent instrumentation e An integral blower hour meter tracks operating life for predictive maintenance planning e To prevent fan damage a paper catch gril
79. e following test methods Inflow downflow velocity PAO aerosol challenge for filter integrity Light noise and vibration Airflow pattern visualization Electrical safety to IEC61010 1 Kl Discus containment Additional microbiological testing is performed on statistical sampling basis ESEO WORLD CLASS WORLDWIDE 10 Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and international criteria Testing in our microbiology laboratory is conducted according to NSF ANSI 49 EN 12469 and JIS K3800 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all testing work Harmless Bacillus atrophaeus formerly Bacillus Subtilis bacteria is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Units CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone Bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet workzone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B atrophaeus spores is placed inside the
80. e of airborne contaminants including Fine dust Smoke Bacteria typical size 500 to 0 3 micron Soot Pollen Radioactive particles Impurity ion gt can affect Integrated Circuit speed gt o gt o Oo o 10 A Guide To Biosafety And Biosafety Cabinets HI Proper Selection Installation amp Safe Use Of BSCs A Selection of A Biological Safety Cabinet A BSC should be selected primarily in accordance with the type of protection needed product protection personnel protection against Risk Group 1 4 microorganisms personnel protection against exposure to radionuclides and volatile toxic chemicals or a combination of these Table 3 shows which BSCs are recommended for each type of protection Table 3 Biohazard Safety Cabinet Selection Type of protection BSC Selection Personnel Protection microorganisms in Risk Class I Class II Class III Groups 1 3 Personnel Protection microorganisms in Risk Class III Groups 4 glove box laboratory Personnel Protection microorganisms in Risk Class I Class II Groups 4 suit laboratory Class II Class III only if laminar Product Protection flow included Volatile radionuclide chemical protection Class IIB1 Class IIA2 vented to the minute amounts outside Volatile radionuclide chemical protection Class I Class IIB2 Class III Source Laboratory Biosafety Manual Second Edition revised Interim guidelines World Health Organization p29 Volatile or toxic chemicals
81. e provisions on each sidewall one provision on each sidewall for 9 meter 3 cabinet Electrical outlets are mounted below The one piece stainless steel side wall is radiused on the corner and easy to clean without crevices or joints Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated All key components with the exception of the motor blower assembly are mounted outside the air stream and away from contaminated air to permit service without decontamination These include fluorescent lamps UV lamp electrical harnesses electronic boards and microprocessor control Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color coded red to warn service technician The telescoping Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when Work area containment is maintained even when removable components are lifted out for cleaning The lower drain trough is a single piece fabrication with wide open angles and a channel 8 N gt 955 mm 1260 mm 1565 mm 1870 mm 37 6 49 6 61 6 73 6 1115 mm 1420 mm 7 1725 mm 2030 mm Model FC2 Biological Safety Cabinet Technical Specifications 1540 mm 60 6 610 mm 24 Exhaust filter Blower Downflow filter Standard UV light Retrofit Kit provision Standard V Bar Retrofi
82. e same side wall ESCO WORLD CLASS WORLDWIDE General Specifications Airstream Class II Biological Safety Cabinets S Series Note to customer Insert electrical voltage number into last model number digits _ when ordering Model Nominal Size External Dimensions WxDxH Internal Work Area Dimensions WxDxH Internal Work Area Space Ne Naa ntlow 0 9 meters 3 1035 x 740 x 1360 mm 40 7 x 29 1 x 52 9 1 2 meters 4 1340 x 740 x 1360 mm 52 7 X 29 1 x 52 9 AC2 3S_ AC2 4S_ AC2 5S_ 1 5 meters 5 1645 x 740 x 1360 mm 64 7 x29 1 x 52 9 AC2 6S _ 1 8 meters 6 1950 x 740 x 1360 mm 76 7 x29 1 x 52 9 965 x 560 x 670 mm 38 0 x 22 0 x 26 4 1270 x 560 x 670 mm 50 0 x 22 0 x 26 4 1575 x 560 x 670 mm 62 0 22 0 26 42 1880 x 560 x 670 mm 74 0 x 22 0 x 26 4 0 43 m 4 7 sq ft 0 58 m 6 2 sq ft 0 73 m 7 7 sq ft 0 87 m 9 3 sq ft 0 45 m s 90 fpm at initial setpoint audible visual alarm will activate at 0 40 m s 80 fom Velocity Downflow 0 30 m s 60 fpm at initial setpoint with uniformity of better than 20 Inflow 270 m3 h 159 cfm 356 m h 209 cfm 441 m h 260 cfm 527 m h 310 cfm iade Ae Wr Downflow 563 m h 331 cfm 741 m3 h 436 cfm 919 m h 591 cfm 1096 m h 645 cfm Exhaust 270 m h 159 cfm 356 m h 209 cfm 441 m h 260 cfm 527 m h 310 cfm
83. ed motor speed controller Reduced height for low ceiling laboratories Extremely low noise level for quiet working environment Laminated shatter proof sliding sash for extra protection W Technical Specifications and Engineering Diagram Esco Catalogue Revision LP2 v1 6 2006 Specifications subject to change without notice 2006 Esco Micro Pte Ltd 3 in NO we Q E NO M e O NO N O N NO i 1204 mm 47 4 555 mm 21 9 _ 1330 mm 52 4 _ 795 mm 31 3 Engineering Details 1 Exhaust ULPA Filter 2 Blower 3 Downflow ULPA filter 4 Standard UV Light Retrofit Kit Provision 5 IV Bar Retrofit Kit Provision 6 Electrical Outlet Retrofit Kit Provision 2 single outlets Z Electrical Electronics Panel 8 Fluorescent Light 9 Plugged Service Fixture Provisions 2 on each side wall for Gas Vacuum Water Solenoid Gas Valve Option Available 10 Stainless Steel Multi piece Work tray 11 Stainless Steel Armrest 12 Esco Sentinel Microprocessor Control System 13 Laminated Shatter Proof Sliding Sash Window 14 Single piece Stainless Steel Back Wall and Side Wall 15 Removable Side Panel for Plumbing Access General Specifications LP2 4AX External Dimensions L x W x H 1330 x 795 x 1486 mm 52 4 x 31 3 x 56 5 Internal Work Zone L x W x H 1204 x 555 x 670 mm 47 4 x 21 9 x 26 4
84. egative pressure IB Positive pressure The Esco triple wall design creates a Dynamic Cham ber plenum which surrounds contaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure The third wall conceals utilities e External surfaces are coated with Esco Isocide antimicrobial coating to protect against surface contamination and in hibit bacterial growth lsocide eliminates 99 9 of surface bacteria within 24 hours of exposure e There are no screws in the front or sides to trap contaminants or complicate cleaning Service Fitting Access The cabinet is pre plumbed for easy instal lation optional gas and vacuum fittings see Accessories e The Service fitting openings are offset for easier access Downtlow Fail 1B If the supply blower fails 1b downflow to the work area is suspended The exhaust blower ramps up to maximum power to provide inflow to the cabinet to help maintain containment The control panel warns of downflow failure With the supply blower offline the cabinet still provides protection by maintaining inflow above 0 40 m s as required by EN 12469 e The External plumbing is concealed behind trim panels to preserve cabinet aesthetics e A normally closed gas solenoid valve automatically shuts off g
85. el microprocessor supervises all cabinet functions Ergonomically angled front improves reach and comfort Actual work opening is 25 4 mm 1 larger than tested sash opening to provide additional work space Frameless shatterproof sash is easier to clean offers larger unobstructed viewing area Removable one piece work surface simplifies cleaning applicable to AC2 S series and AC2 D series E Series cabinets include multi piece tray components which lift and remove to provide easy access encourage surface decontamination and can be sterilized inside autoclave Raised airflow grille maintains safety by preventing blockage Airstream E Series Class II Biological Safety Cabinet features glass sides to enhance visibility inside the work area Model AC2 4E_ Airstream S Series Class II Biological Safety Cabinet with stainless steel side i i del AC2 45_ Airstream AC2 Series Model Number Side Wall Configuration c l f Stainless Steel Stainless Steel Cabinet Size Glass E Series Blo DUSeriee 0 6 meters 2 ACES NIL NIL 0 9 meters 3 AC2 3E_ AC2 3 _ NIL 1 2 meters 4 AC2 4E_ AC2 4S _ AC2 4D_ 1 5 meters 5 AC2 5E_ AC 252 NIL Note The last digit of the Model Number _ indicates desired sss s NCLB AC2 65_ AC2 6D_ electrical service 60Hz 50Hz See the Specification Chart for details Specify voltage Hz when ordering Airstream Biological Safety Cabinet
86. elocity PAO aerosol challenge for filter integrity Light noise and vibration Airflow pattern visualization Electrical safety to IEC61010 1 Additional microbiological testing is performed on statistical sampling basis ESCO D OOO CLASS WORLDWIDE ESCO Airstream E Series Class II Biosafety Cabinet 2 feet version A Compact Size For All Laboratories Width 2 4 73cm Total Widi 2 66cm Lee Internal MAIN FILTRATION AGENTS m ISO Class 3 air cleanliness within workzone as per ISO 14644 1 equivalent to Class 1 as per the US Federal Standard 209E 100 times cleaner than the usual Class 100 classification on cabinets offered by the competition m Minipleat separatorless ULPA filter technology operating at the typical efficiency of 99 9999 at MPPS 0 3 and 0 12 microns provides better operator product and environmental protection than conventional HEPA filters This cabinet employs 2 ULPA filters 1 for downflow and 1 for exhaust airflow filtration CABINET CONTROL SYSTEM Esco Sentinel Microprocessor Cabinet Control System allows the user to easily access all cabinet functions The control system monitors and displays cabinet airflow constantly on the control LCD screen prompting the user with audible and visual alarms in case of any unsafe condition m Fail safe system ensures that in case of airflow failure the cabinet automatically shuts down to ensu
87. eme left and right side Combined with the side air gap between the work tray and the side wall the stronger side capture zones increase protection in this critical area where contaminants tend to bleed out in conventional cabinet e The inflow grille has a convex shape to maintain operator protection by discouraging users from placing objects on it e Raised armrest prevents the likelihood of inflow grille blocking by operator s arms e Auto purge holes located at the front side walls eliminate eddy currents and dead air pockets in the critical area behind the sash window Per NSF ANSI 49 requirement these side perforations do not extend completely to the work surface to prevent accidental liquid spills in the work zone from entering the side air column e The ULPA downflow supply filter is tilted proportional to the cabinet front angle to direct more air forward to the front air grille e The inflow velocity downflow velocity and air flow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front aperture this curtain maintains personal and product protection even in the unlikely event of a severe inflow or downflow imbalance that would compromise protection in a conventional cabinet Integrated Filtration System A combination of a supply ULPA filter and an exhaust HEPA filter give the Labculture cabinet a fully integrated performance envelope for product operato
88. ement these side perforations do not extend completely to the work surface to prevent accidental liquid spills in the work zone from entering the side air column e The ULPA downflow supply filter is tilted proportional to the cabinet front angle to direct more air forward to the front air grille e The inflow velocity downflow velocity air flow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front aperture this curtain maintains personnel and product protection even in the unlikely event of a severe inflow or downflow imbalance that would compromise protection in a conventional cabinet Integrated Filtration System A combination of a supply ULPA filter and an exhaust ULPA filter give the Labculture cabinet a fully integrated performance envelope for product operator and environmental protection e ULPA filters per IEST RP CC001 3 are tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron particles these provide better filtration capability than conventional HEPA filters that have a typical efficiency of gt 99 99 for 0 3 micron particles Mini pleat Separatorless Filter left vs Conventional Aluminium Separator Filter right Esco cabinets use Swedish Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the chance of leakage and to prolong filter life Filters include a lightweight aluminum frame
89. ervises all cabinet functions Setpoints and other applications are user activated through touch pad programming access detailed in the Operations Manual A key switch located on the cabinet prevents unauthorized use of the cabinet The motorized sash is controlled by an up down button A data output for remote monitoring and information management to meet FDA21CFR and other criteria is available contact Esco or your Sales Representative for details A pressure sensor monitors pressure drop across the filter plenum and com putes estimated remaining filter life A one touch half speed setting can be activated at the end of the work day to reduce energy consumption prolong filter life and maintain interior work zone cleanliness for the next day s work All electronic parts are contained inside a plug and play module that permits easy exchange If required Microprocessor software updates are available from Esco for download via the Internet Sentinel functions are factory set to default to ON or OFF depending on worldwide destination and local pref erences Default settings can be user activated through the touchpad data entry access Esco Accuflow Microprocessor Speed Controller Blower Speed from mechanical dam age by an inte The Esco Accuflow microprocessor speed controller maintains steady motor blower speed despite grated metal screen guard which is containment and protection absent fro
90. et Retrofit Kit provision 10 IV bar Retrofit Kit provision 2 Blower 2 on each side 2 amp 3 ft model only 1 single outlet 11 Esco Sentinel microprocessor 4 Standard UV light Retrofit Kit 8 Electrical panel 12 Sash window Provision 9 Fluorescent lamp quiet vibration free operation Airstream Biological Safety Cabinet e Class II Microbiological Safety Cabinets E Series S Series and D Series Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right Esco Airstream cabinets use a combination of high performance scroll blowers supply and German made ebm papst permanently lubricated centrifugal motor blowers with external rotor designs exhaust Selected for energy efficiency compact design and flat profile the completely integrated exhaust blower assembly optimizes motor cooling with unified rotating parts and overall component balance for smooth Weight is equally distributed to all bearings to extend bearing life transfer heat and maximize speed control Airstream Model AC2 S Series Biological Safety Cabinet Technical Specifications Stainless Steel Side Walls 1360 mm 52 9 0 1035 1340 1645 1950 mm 40 7 52 7 64 7 7 76 7 560 mm 22 740 mm 29 1 1 Exhaust ULPA filter 5 Plugged service fixture provisions Z Electrical outlet Retrofit Kit provision 10 IV bar Retrofit Kit provision
91. evices or joints The lower drain trough is a single piece fabrication with wide open angles and a channel to direct spills to the drain The closed sidewall contains no perforations air return slots or other hidden areas where contaminants can accumulate ESO WORLD CLASS WORLDWIDE Biosafety Cabinets Standards Compliance Dynamic Chamber Plenum Design E High negative pressure E Negative pressure W Positive pressure The Esco triple wall design creates a Dynamic Chamber plenum which surrounds contaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure The third wall conceals utilities Comfortable Ergonomic Design The LB2 cabinet is engineered for comfort utility value and safety e The 10 angled viewing window and narrow profile front grille improves reach into the work area e The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy e The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Technical Specifications NSF ANSI 49 USA LB2 4B2 LB2 6B2 SFDA YY 0569 China Air Quality ISO 14644 1 Class 3 Worldwide JIS B9920 Class 3 Japan JIS BS5295 Class 3 Japan US Fed Std 209E Class 1 USA e The front armrest is raised above the workzone to improve
92. external blower the cabinets internal blower will blow the air and microbiological agents inside the work zone through the front operator towards the operators face creating a dangerous situation This cabinet is not self balancing in the sense that its own blower can only create downflow and the cabinet relies on the external blower to create inflow A Guide To Biosafety And Biosafety Cabinets On all Type B cabinets environmental protection may be enhanced by installing a scrubbing system between the exhaust of the biohazard and the final exhaust point outside the building to neutralize the chemical fumes present in exhaust air Although Type B cabinets are commonly used when chemicals are involved in your work processes they theoretically provide an increased level of safety as compared to other Type A cabinets By exhausting air directly to the external environment they provide an additional fail safe in the event that the regular exhaust HEPA filtration ceases to function Class II Type B1 Biohazard Safety Cabinet The Class II Type B1 biohazard safety cabinet was originally specified by the American National Cancer Institute It has a common plenum from which 70 of air is exhausted and 30 re circulated to the work area as the downflow Type B1 cabinets also have a dedicated exhaust feature that eliminates re circulation when work is performed towards the back within the interior of the cabinet Toxic chemicals employed a
93. f surface bacteria within 24 hours of exposure e The drain pan is flush with the side walls to eliminate concealed or hard to clean spaces e The interior work area is formed from a single piece of stainless steel with large radius corners to simplify cleaning Robust Cabinet Construction and Enhanced Safety Features Service fixtures are offset for easier reach Standard cabinets include two fixture provisions on each sidewall one provision on each sidewall for 0 9 meter 3 ft cabinet Electrical outlets are mounted below service fixtures to minimize obstructions Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated gas spring struts providing front service access All key components with the exception of the blower motor assembly are mounted outside the air stream and away from contaminated air to permit service without decontamination These include fluorescent lamps UV lamp electrical harnesses electronic boards and microprocessor control Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color coded red to warn service technician The Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when required Work area containment is maintained even when removable components are lifted out for cleaning The one piece stainless steel work tray edges are radiused and easy to clean without cr
94. gical safety cabinets EN 12469 as a test method for validating the operator personnel protection capabilities of the cabinet e The KI Discus test shows excellent correlation with the microbiological test method for operator protection and is useful for validating the actual containment performance of the cabinet on site e The Kl Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus each Esco Labculture LB2 model is factory tested using the KI Discus method for operator safety Purchase Specifications LB2 Series Class Il Type B2 Total Exhaust Biological Safety Cabinet General Performance and Certifications 1 The biological safety cabinet shall comply with one or more of the following international standards and the manufacturer shall provide a certified copy of containment and performance tests equivalent to or greater than specified in the following independent international Standards for biological safety electrical and other functional characteristics Class Il Type B2 per NSF ANSI 49 USA 2 The cabinet shall protect a the operator and laboratory environment from particulates generated within the work zone b the product and process within the work zone from airborne contamination from ambient air c and the product and process within the work zone from cross contamination 3 Before shipment each cabinet shall be individually tested by the KI Discus test Euro
95. gral metal guards and filter frame gaskets fully compliant with EN 1822 and IEST RP yp CC001 3 requirements Typical efficiency 99 9997 at MPPS 99 9998 at 0 3 and 0 12um Noice asaj According to EN12469 lt 61 dBA lt 65 dBA The above measurements were taken at initial blower speed setting figures subject to acoustic properties of test environment Light Intensity gt 1000 Lux gt 93 foot candles measured at work surface level zero background as per NSF49 test grid Maximum Usable Workzone Depth 510 mm 20 does not include the 110 mm 4 3 front air grille Maximum Usable excludes front air 0 64 sqm 6 89 sqf 0 95 sqm 10 22 saf Knee Space Depth 245 mm 9 6 1 5mmt 0 06 16 gauge electro galvanised steel with white oven baked epoxy anti microbial Isocide Maim Body Pn powder coated finish 3mm 0 12 lead shielding on cabinet side walls and work zone bottom Maximum 220 240VAC Cabinet 675W 4A 765W 4A Power 50Hz 1Ph Outlet 1000VA 5A 1000VA 5A Consumption 110 130VAC Cabinet 835W 11 5A 1045W 11 5A Current 60Hz 1Ph Outlet 500VA 5A 500VA 5A Your Local Distributor Esco Biotechnology Equipment Division x lt Q 21 Changi South Street 1 Singapore 486777 Tel 65 6543 0833 Fax 65 6542 6920 Email biotech escoglobal com Website biotech escoglobal com Philadelphia PA USA Houston TX USA Leiden The Netherlands Yokohama Japan Bei
96. gt 1400 Lux gt 1070 Lux gt 1225 Lux p y gt 114 foot candles gt 130 foot candles gt 100 foot candles gt 114 foot candles NSF ANSI 49 Certified Pending Yes Yes Yes Electrogalvanized steel with Isocide oven baked epoxy polyester powder coating 1 2 mm 0 05 18 gauge 1 5 mm 0 06 16 gauge 1 2 mm 0 05 18 gauge 1 5 mm 0 06 16 gauge Cabinet Construction Electrical 110 130V AC 60Hz 1 Net Weight 243 kg 536 Ibs 336 kg 741 Ibs 317 kg 698 Ibs 434 kg 957 Ibs Shipping Weight 292 kg 644 Ibs 400 kg 880 Ibs 410 kg 904 Ibs 523 kg 1153 Ibs Shipping Dimensions 1230 x 940 x 1900 mm 1530 x 940 x 1900 mm 1910 x 940 x 1900 mm 2150 x 940 x 1980 mm Maximum W x D x H 48 4 x 37 x 74 8 60 2 x 37 x 74 8 75 2 SEI XTAB 84 6 x37 x 77 9 Shipping Volume Maximum 2 20 m 78 cu ft 2 73 m 97 cu ft 3 41 m 121 cu ft 4 00 m 141 cu ft Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Accessories and Options Cont Cabinet Accessories e Ergonomic lab chair e Cradle stand electrical hydraulic e Germicidal UV lamp Laboratory grade construction infinitely adjustable with casters Controlled by automatic UV lamp meets Class 100 cleanliness alcohol Elevates to seating or standing timer through Sentinel micro resistant PVC materials work surface height processo
97. hanges and before being moved The most common decontamination method is by fumigation with formaldehyde gas BSC decontamination should be performed by a qualified professional A Guide To Biosafety And Biosafety Cabinets 11 Personal protective equipment Personal protective clothing should be worn whenever using a BSC Laboratory coats are acceptable for work being performed at Biosafety Levels 1 and 2 A solid front back closing laboratory gown provides better protection and should be used at Biosafety Levels 3 and 4 except for suit laboratories Gloves should be pulled over the wrists of the gown rather than worn inside Elasticized sleeves can be worn to protect the investigator s wrists Masks and safety glasses may be required for some procedures 12 Alarms BSCs can be equipped with one of two kinds of alarm Sash alarms are found only on cabinets with sliding sashes The alarm signifies that the operator has moved the sash to an improper position Corrective action for this type of alarm is returning the sash to the proper position Air flow alarms indicate a disruption in the cabinet s normal air flow pattern This represents an immediate danger to the operator or product When an air flow alarm sounds work should cease immediately and the laboratory supervisor should be notified Manufacturers instruction manuals should provide further details Training in the use of BSCs should cover this aspect 13 Supplementary Inform
98. hin and throughout the work area Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow intake ae ceca at bbe ture zones sma all blue arre VS e Built in RFI and electrical noise filters eliminate interference with adjacent instrumentation e An integral blower hour meter tracks operating life and aids in predictive maintenance planning e To prevent fan damage a paper catch grille traps papers or towels that may drop down on the drain pan preventing them from being pulled into the column by fan suction Sentinel Microprocessor Control Alarm Monitoring System The Esco Sentinel microprocessor based control system supervises operation of all cabinet functions Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right Labculture Esco cabinets use German made ebm papst permanently lubricated centrifugal motor blowers with external rotor designs Integrated blades norrow the profile and eliminate the need for a motor shaft Motors are selected for energy efficiency compact design and flat profile The completely integrated assembly optimizes motor cooling All rotating parts are unitized and balanced for smooth quiet vibration free operation Biological Safety Cabinet e Class Il Type B2 Total Exhaust Bi
99. iciency gt 99 999 at particle size between 0 1 to 0 3 microns NSF ANSI 49 lt 66 dBA lt 63 5 dBA lt 66 dBA lt 67 dBA Sound Emission EN 12469 lt 63 dBA lt 60 5 dBA lt 63 dBA lt 64 dBA EMO Sem sss anat gt 1230 Lux gt 1400 Lux gt 1070 Lux gt 1225 Lux p y gt 114 foot candles gt 130 foot candles gt 100 foot candles gt 114 foot candles Electrogalvanized steel with Isocide oven baked epoxy polyester powder coating Cabinet Construction 1 2 mm 0 05 18 gauge LA2 3A1 LA2 4A1 LA2 5A1 LA2 6A1 50Hz 1 Electrical LA2 3A3 LA2 4A3 LA2 5A3 LA2 6A3 60Hz 1 100V AC ety LA2 LA2 4 LA2 LA2 50 60Hz 10 3 5 J5 5J5 6J5 Net Weight 243 ko 536 Ibs 283 kg 624 Ibs 317 ko 698 Ibs 350 ko 772 Ibs Shipping Weight 292 kg 644 Ibs 345 ko 761 Ibs 410 kg 904 lbs 486 kg 1072 lbs Shipping Dimensions 1230 x 940 x 1900 mm 1530 x 940 x 1900 mm 1910 x 940 x 1900 mm 2150 x 940 x 1980 mm Maximum W x D x H 48 4 x 37 x 74 8 60 2 x 37 x 74 8 75 2 x 37 x 74 8 84 6 x37 x 77 9 Shipping Volume Maximum 2 20 m 78 cu ft 2 73 m 97 cu ft 3 41 m 121 cu ft 4 00 m 141 cu ft Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Comprehensive Performance Testing At Esco Every Labculture LA2 model manufactured by Esco is individually tested documented by serial number and validated with th
100. ing the motor blower system is able to compensate automatically to maintain airflow as the filter is loaded with particulates m Built in solid state variable speed controller s infinitely adjustable from zero to the maximum setting with built in RFI and noise filters is superior to conventional step controllers m Superior electrical safety for the operator all electrical components are UL listed or UL recognized all cabinets are factory tested for electrical safety after production at manufacturing site Designed to meet the general safety requirements of the IEC 61010 1 EN 61010 1 UL 61010A 1 CSA C22 2 No 1010 1 92 OPTIONAL ACCESSORIES Support Stand with castor wheels or levelling feet Cabinet construction allows for the cabinet to be lifted directly on to our optional support stand available 2 with castor wheels or levelling feet A Exhaust collar for non airtight thimble ducting of the cabinet For other optional and ergonomic accessories check our catalogue http biotech escoglobal com PDF optErgAcc pdf Esco Catalogue Revision No AC2 2ft v1 12 2005 Specifications subject to change without notice 2005 Esco Micro Pte Ltd Technical Specifications and Engineering Diagram 173 mm 6 8 1435 mm 56 5 670 mm 26 4 Work zone heignt _ 660 mm 26 0 732 mm 28 8 _ 730 mm 28 7 1 Exhaus
101. ings openings are offset for easier access Comfortable Ergonomic Design The cabinet is engineered for comfort utility value and safety e The angled viewing window and narrow profile front grille improves reach into the work area e The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy e The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Specifications e The front armrest is raised above the work zone to improve comfort and to minimize blockage of forward airflow perforations e The optional adjustable support stand provides work surface height control e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone pro vides ample room for transferring of small equipment see Specifications e The sliding window can be fully opened to insert and remove larger instrumentation and equipment A graphical interface indicates cabinet performance Digital read out with alpha numeric display indicates all input status and alarm functions All functions can be user activated through touch pad programming access see Operations Manual Read all sataty related instructions batore use Toast certify this line r i tie imi P P I a LEE TY Factura Er Bl tie 1 LAnda
102. istant finish IV bar with hooks Stainless steel construction Available for all standard Esco cabinets Microscope viewing device Mounting and viewing pouch integrated into sash Factory installed specify when ordering ESCO WORLD CLASS WORLDWIDE General Specifications x Nominal Size External Dimensions WxDxH B Gross Internal Dimesnsions WxDxH 40 7 x 33 3 x 61 7 1420 x 815 x 1568 mm DAE X SE X GITA LB2 4B_ LB2 5B_ 0 9 meters 3 1 2 meters 4 1 5 meters 5 1115 x 815 x 1568 mm 1725 x 815 x 1568 mm 64 7 x 33 3 x 61 7 LB2 6B_ 1 8 meters 6 2030 x 815 x 1568 mm 80 0 x 33 3 x 61 7 955 x 603 x 715 mm BLO X213 X281 1260 x 623 x 715 mm 49 6 x 24 5 x 28 1 1565 x 603 x 715 mm 6G X213 25 12 1870 x 603 x 715 mm T36 X213 x 2 1 Usable Work Area 0 44 m 4 7 sq ft 0 59 m 6 4 sq ft 0 74 m 7 9 sq ft 0 89 m 9 6 sq ft Tested Opening 203 mm 8 203 mm 8 203 mm 8 203 mm 8 Working Opening 248 mm 9 8 248 mm 9 8 248 mm 9 8 248 mm 9 8 Average Airflow low 0 53 m s 105 fpm Velocity Downflow 0 35 m s 70 fpm Inflow 368 m h 217 cfm 489 m h 288 cfm 608 m h 358 cfm 725 m h 427 cfm Downflow 702 m h 413 cfm 924 m h 544 cfm 1147 m h 675 cfm 1369 m h 806 cfm Exhaust 1074 m h 632 cfm 1415 m h 833 cfm 1755 m h 1033 cfm 2097 m h 1234 cfm
103. ize containment Work area on Esco 0 9 meter 3 cabinet is equivalent to work area offered on larger 1 2 meter 4 conventional cabinet Each cabinet is KI Discus tested for performance integrity Available in 0 9 1 2 1 5 and 1 8 meter models 3 4 5 and 6 Shown with optional telescoping stand w Dus SFDA Esco Labculture has passed more performance tests in more languages for more certifications throughout more countries than any other biological safety cabinet in the world Labculture Biological Safety Cabinet e Class II Type B2 Total Exhaust Biological Safety Cabinets Leabculture Operator Product and Environmental Protection The Esco Labculture Class ll Type B2 Biosafety Cabinet provides operator product and environmental protection against Biosafety Levels 1 2 and 3 This cabinet can be used for handling Biohazard Level 4 provided that the operator wears positive pressure sult Containment and Protection e The total exhaust design of the LB2 makes the cabinet suitable for microbiological work involving trace amounts of toxic chemicals e The non recirculating design guarantees safety in cases where exhaust HEPA filtration fails to ensure containment e Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination e The front grille has proportionally larger perforation on the extr
104. jing China Shanghai China Kuala Lumpur Malaysia Mumbai India Dubai U A E Infinity gt yy Esco Infinity tested to EN 12469 and TUV GS certified for safety and performance Model FC2 4A_ with optional support stand Class II Microbiological Safety Cabinet The Industry s Premier Biological Safety Solution from Esco E INITY ade ailin LEWE RE Haine u Main Features Unique Dynamic Chamber plenum with angled filter delivers superb airflow uniformity with deviation no greater than 5 to 6 of average downflow exceeding European Standard EN 12469 by more than three times Negative pressure plenum surrounds contaminated positive pressure plenum no fabric bags are used Dual fan design for guaranteed safety If one fan fails minimal protection is still maintained with only one fan running i Esco next generation Sentine supervises all cabinet functions microprocessor A large easy to read digital display and ergonomically sized touchpad controls im prove user interface Fully closable motorized sash provides an airtight seal for better safety when cabinet is inoperative overnight Frameless shatterproof sash is easier to clean offers larger unobstructed viewing area Ergonomically angled front improves reach and comfort Multi piece work surface removal simplifies cleaning Raised armrest maintains safety by preventing blockage R oY A T
105. l Specifications External Dimensions L x W x H AB2 4SX 1340 x 710 x 1460 mm 52 8 x 28 0 x 57 5 AB2 6SX 1950 x 710 x 1460 mm 76 8 x 28 0 x 57 5 Internal Work Zone L x W x H 1270 x 550 x 670 mm 50 0 x 21 7 x 26 4 1880 x 550 x 670 mm 74 0 x 21 7 x 26 4 Designed to meet and exceed the requirements of EN 12469 2000 and ANSI NSF49 Standards Air cleanliness ISO 14644 1 Class 3 IEST G CC1001 IEST G CC1002 and other equivalent requirements Filter performance IEST RP CC034 1 IEST RP CC007 1 IEST RP CC001 3 and EN1822 Electrical safety IEC 61010 1 EN 61010 1 UL 61010A 1 CSA C22 2 No 1010 1 92 Standards Compliance Average Airflow Inflow Initial setpoint 0 53 m s or 105 fom Velocities Downflow Initial setpoint 0 31 m s or 61 fom uniformity is 20 Inflow 419 cmh 246 cfm 621 cmh 365 cfm Airflow Volumes Downflow 897 cmh 528 cfm 1327 cmh 781 cfm At Initial Airflow Velocity Setpoints Total Exhaust 1166 cmh 686 cfm 1714 cmh 1008 cfm Audible visual alarm will activate when exhaust airflow volume drops below 12 of the initial volume Pressure Drop Requirements for Exhaust Ducting 440 Pa 1 77 WG 365 Pa 1 47 WG ISO 14644 1 Class 3 US Federal Standard 209E Class 1 M1 5 AS 1386 Class 1 5 JIS B9920 Class 3 BS5295 Class C Class M10 000 as per KS 27030 1 and equivalent classes of VDI 2083 and AFNOR X44101
106. le items UI Inside The Centrifuge Clear area of all personnel Wait 30 minutes for aerosol to settle before attempting to clean up spill Wear a lab coat safety glasses and gloves during cleanup Remove rotors and buckets to nearest biological safety cabinet for cleanup Thoroughly disinfect inside of centrifuge Discard contaminated disposable materials using appropriate biohazardous waste disposal procedures e g autoclave or BFI e x a gt 18 A Guide To Biosafety And Biosafety Cabinets D Outside The Lab In Transit Transport labelled biohazardous material in an unbreakable well sealed primary container placed inside of a second unbreakable lidded container cooler plastic pan or pail labelled with the biohazard symbol Should a spill occur in a public area do not attempt to clean it up without appropriate personal protective equipment Secure the area keeping all people well clear of the spill Call professional help to assist in cleanup Stand by during spill response and cleanup activity and provide assistance only as requested or as necessary gt From UCSD Biosafety handbook UCSD EH amp S Biosafety Team 1996 THE UNIVERSITY OF CALIFORNIA USA p95 97 V References 1 User amp Service Manual Labculture Class II Type A2 Biohazard Safety Cabinet ESCO Micro Pte Ltd 2 Biosafety in Microbiological and Biomedical Laboratories BMBL 4th Edition Office of Hea
107. le traps papers or towels that may drop down on the drain pan preventing them from being pulled into the column by fan suction Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right Labculture Esco cabinets use German made ebm papst permanently lubricated centrifugalmotor blowers with external rotor designs Integrated blades narrow the profile and eliminate need for a motor shaft Motors are selected for energy efficiency compact design and flat profile The completely integrated assembly optimizes motor cooling All rotating parts are unitized and balanced for smooth quiet vibration free operation Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets m Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone Air returns to the common air plenum where the 35 exhaust and 65 recirculation process is continued Sentinel Microprocessor Control Alarm Monitoring System The Esco Sentinel mic
108. le wall design whereby all positive pressure plenums capable of handling contaminated air shall be surrounded by negative pressure No positive pressure areas shall be accessible external to the cabinet The third wall shall conceal utilities The cabinet shall maintain containment performance even when removable work area components are removed for cleaning The work tray shall be one piece removable stainless steel with radius corners without crevices or joints The cabinet shall have a stainless steel one piece fabricated drain trough with open angles to channel spills to a common drain The closed sidewall shall be sealed without perforations return air slots or concealed areas which can contain contaminants The cabinet shall be free of sharp edges non functional protrusions bolts screws or hardware and all metal edges shall be deburred Ergonomics and Convenience 24 25 The front sash shall be frameless to maximize visibility and accessible for cleaning front and back Sash glass shall be safety glass The sash counterbalance shall be suspended on two high strength cables and the sash shall lock into position in the event one cable becomes detached Biological Safety Cabinet e Class Il Type B2 Total Exhaust Biological Safety Cabinets 26 27 28 29 30 31 32 33 Magnetic not mechanical proximity sensors shall work in conjunction with the control system to indicate p
109. lenum blower plenum at the top of the cabinet The uniform non turbulent air stream protects against cross contamination within and throughout the work area Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille J ULPA filtered air A small portion of the ULPA filtered C Unfiltered potentially contaminated air downflow enters the intake perforations at the side capture zones at a higher Room air Inflow air a zi L velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone Air returns to the common air plenum where the 35 exhaust and 65 recirculation process is continued Airstream Model AC2 E Series Biological Safety Cabinet Technical Specifications Stainless Steel Side Walls l o o I fi e o g E Fed 11 k Ca E 1 a a 2 M A Pa fy j ji N I i a u i 4 560 mm 22 k 1035 1340 1645 1950 mm i 740 mm 29 1 40 7 f 52 7 f 64 7 76 7 1 Exhaust UL PA filter 5 Plugged service fixture provisions Z Electrical outl
110. lenums for additional safety Unique airflow technology maintains cabinet negative pressure at 274Pa 1 1inWater within the chamber under all circumstances for maximum safety and containment Designed to meet the safety requirements of IEC 61010 1 EN 61010 1 UL 3101 1 CSA C22 2 No 1010 1 92 Components are UL listed recognised Extended warranty period of 3 years excluding consumable parts and accessories General Features Cont Class III Cabinet Airflow Profile INDIVIDUAL PERFORMANCE TESTING Your safety means the world to us the following tests are i performed individually at our in house laboratory on every cabinet produced a Airflow velocities b Cabinet leak test c Aerosol challenge test for filter integrity d Light intensity noise vibration levels e Airflow pattern visualisation test f Electrical safety tests A detailed report is included with every cabinet detailing the tests performed and results obtained Extensive testing is continuously performed at our research and development laboratories to improve cabinet containment and performance Recommended re certification is 12 months from initial date of operation in addition we recommend that where possible on site testing after delivery and installation should be conducted in accordance with the following standard EN12469 2000 Section Exhaust Air is ULPA filtered Intake Air Pre filter Removes Larger Particles
111. llenge for filter integrity Light noise and vibration Airflow pattern visualization Flectrical safety to EC61010 1 KI Discus containment Additional microbiological testing is performed on statistical sampling basis Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and international criteria Testing in our microbiology laboratory is conducted according to NSF ANSI 49 EN 12469 and JIS K3800 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all tests Harmless Bacillus atrophaeus formerly Bacillus Subtilis bacteria is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Units CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone Bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet workzone e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B atrophaeus spores Is placed inside the workzone 10 cm 4 inches behind the front opening sash e Target slit air samplers and impingers are placed
112. lth and Safety OHS Centers for Disease Control and Prevention CDC US Department of Health 3 Laboratory Biosafety Manual Second Edition revised Interim guidelines World Health Organization 4 UCSD Biosafety handbook UCSD EH amp S Research Community for Biosafety 1996 5 UCSD Biosafety handbook UCSD EH amp S Biosafety Team 1996 THE UNIVERSITY OF CALIFORNIA PRODUCTS AVAILABLE FROM ESCO BIOTECH Vertical Laminar Flow Cabinets Horizontal Laminar Flow Cabinets PCR Vertical Laminar Flow Cabinets Class II Biosafety Cabinets Class III Biosafety Cabinets Cytotoxic Cabinets IVF Cabinets Weighing Cabinets Animal Handling Workstations Custom Made Clean Air Containment Workstations Your local distributor Esco Biotechnology Equipment Division 21 Changi South Street 1 Singapore 486777 tel 65 6542 0833 fax 65 6542 6920 email biotech escoglobal com website biotech escoglobal com 2005 Esco Micro Pte Ltd Airstream Class II Biological Safety Cabinets The Safety Solution for Life Science Laboratories T 5 m As 4 A Airstream Class Il Biological Safety Cabinet E series Model AC2 4E_ Main Features Unique Esco Dynamic Chamber plenum design delivers quiet uniform airflow Negative pressure plenum surrounds contaminated positive pressure plenum no fabric bags are used Dual long life ULPA filters for supply and exhaust airflow Esco Sentin
113. m conventional HEPA filters building voltage fluctuations thereby assuring constant face velocity and downflow for optimum safety ESO WORLD CLASS WORLDWIDE _ ULPA filtered air L Unfiltered potentially contaminated air L Room air Inflow air e Automatic start up sequence will pre pare the cabinet for normal operation and advise when safe conditions are established e An administrator controlled PIN Per sonal Identification Number can be set to restrict access to main menu e The airflow alarm can be activated or deactivated depending on user prefer ence and nature of the work Consult your Esco Operating Manual or contact Esco or your Sales Representative for information on user preference pro gramming capabilities built into the Sentinel microprocessor platform Redundant Blower System The Infinity blower system is designed for high performance operation redundancy maximum energy efficiency and minimal maintenance Cabin et Fi 1 I Side capture zones Dynamic air barrier inflow and forward directed downflow air converge gm Ambient air is pulled through the perfora tions located towards the work zone front to prevent contamination of the work sur face and work product The inflow does not mix with the clean air within the cabi net work zone Inflow air travels through a return path toward the common air plenum blower plenum at the top of the cabinet Approximately 35
114. m from being pulled into the column by fan suction Dual Blower System Airstream D Series Duo Models The Airstream Duo includes a dual blower system designed for redundancy e Dual permanently lubricated direct drive external rotor motor blowers assure cabinet safety in the event of a motor failure e The external rotor motor design allows for optimum cooling of the motor during extended operations e The double blower system assures inher ently safe operation if one blower fails Front Sash Assembly The front sash is counterbalanced for convenient one hand operation e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equipment see Specifications e The sliding window can be fully opened to insert and remove larger instrumen tation and equipment Sentinel Microprocessor Control Alarm Monitoring System The Esco Sentinel microprocessor based control system supervises operation of all cabinet functions e The control panel is located on the center of the cabinet and angled down for easy access by the operator e Continuous monitoring of cabinet airflow is displayed on a bright easy to read LCD panel The large display monitors operational parameters Independent supply and exhaust filters provide 99 999 typical efficiency for particle sizes of 0 1
115. mic Chamber Plenum Design l Negative pressure IB Positive pressure The Esco triple wall design creates a Dynamic Chamber plenum which surrounds contaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure The third wall conceals utilities Comfortable Ergonomic Design The LA2 cabinet is engineered for comfort utility value and safety e The 10 angled viewing window and narrow profile front grille improves reach into the work area e The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy e The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Technical Specifications ISO SID e The front armrest is raised above the workzone to improve comfort and to ensure that the operator s arms do not block the forward airflow perforations e The optional adjustable support stand provides work surface height control e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equipment e The sliding window can be fully opened to insert and remove larger instrumentation and equipment Electrical Safety and Certification All components meet or exceed applicable safety req
116. need to decontaminate the cabinet 48 All exterior surfaces shall be painted with a permanent antimicrobial inhibitor coating to minimize contamination ESO WORLD CLASS WORLDWIDE 11 Esco Labculture Plus Class Il Type A2 Biosafety Cabinet gt LABCULTURE PLUS a i _ nig SS SU oe Sa Saree The Esco Labculture Plus Class II Type A2 biosafety cabinet is an enhanced version of our internationally certified Labculture Class Il Type A2 biosafety cabinet model Providing operator productandenvironmentprotectionfromhazardous aerosols through superior minipleat ULPA filters the product combines the best product performance features with Esco s ergonomic design The cabinet features Esco Isocide antimicrobial coated external body structure and a reduced overall height for low ceiling laboratories ESCO www escoglobal com a Key Features Pending LNE NF certification to EN 12469 Product Operator Environment Protection ULPA Filters with typical efficiency at gt 99 999 at 0 12um provide ISO Class 3 cleanliness within work zone Easy to Clean Single sheet internal walls Isocide antimicrobial coated structure eliminates 99 9 of bacterial presence on external surfaces within 24 hours Ergonomic sloped front eliminates glare off the front window and ensures operator comfort during extended usage Microprocessor based control and alarm system Esco Accuflow microprocessor bas
117. net The Class I cabinet has the most basic and rudimentary design of all biohazard safety cabinetry available today A stream of inward air moving into the cabinet contains aerosols generated during microbiological manipulations It then passes through a filtration system that traps all airborne particles and contaminants Finally clean decontaminated air is exhausted from the cabinet The filtration system usually consists of a pre filter and a HEPA High Efficiency Particulate Air filter Although the Class I cabinet protects the operator and the environment from exposure to biohazards it does not prevent samples being handled in the cabinet from coming into contact with airborne contaminants that may be present in room air Naturally there is a possibility of cross contamination that may affect experimental consistency Consequently the scope and application of Class I cabinets is limited and it is largely considered obsolete All Class I biohazard safety cabinets are suitable for work with microbiological agents assigned to biological safety levels 1 2 and 3 Class II Biohazard Safety Cabinet Like Class I safety cabinets Class II cabinets have a stream of inward air moving into the cabinet This is known as the inflow and it prevents the aerosol generated during microbiological manipulations to escape through the front opening However unlike Class I cabinets the inflow on Class II cabinets flows through the front inlet grille near
118. ng on worldwide destination and local preferences Default settings can be user activated through the touchpad data entry access e Automatic start up sequence will prepare the cabinet for normal operation and advise when safe conditions are established e An administrator controlled PIN Personal Identification Number can be set to restrict access to main menu e The airflow alarm can be activated or deactivated depending on user preference and nature of the work Touchpad data entry buttons permit control settings and access to diagnostics default Programmable automatic UV light timer simplifies operation enhances A graphical interface indicates cabinet performance settings and hierarchical menus contamination control extends UV lamp life and saves energy Color coded indicator lamps display green for primary function fan operation blue for secondary function fluorescent lights and electrical outlet and orange for caution UV lamp ON Sentinel Microprocessor Control System Programmable Consult your Esco Operating Manual or contact your Sales Representative for information on user preference programming capabilities built into the Sentinel microprocessor platform Cabinet Construction Digital read out with alpha numeric display indicates all input status and alarm functions All functions can be user activated through touch pad programming access see Operations Manual Read all safet
119. nt or facility design is neither required nor generally used Laboratory personnel have specific training in the procedures conducted in the laboratory and are supervised by a scientist with general training in microbiology or a related science A biohazard safety cabinet is generally not required for work involving these agents Biosafety Level 2 Practices safety equipment and facilities appropriate for work done with a broad spectrum of indigenous moderate risk agents present in the community and associated with human disease in varying severity It differs from biosafety level 1 in that a laboratory personnel have specific training in handling pathogenic agents and are directed by competent scientists b access to the laboratory is limited when work is being conducted c extreme precautions are taken with contaminated sharp items and d certain procedures in which infectious aerosols or splashes may be created are conducted in biological safety cabinets or other physical containment equipment A Class I or Class II biohazard safety cabinet is highly recommended for work involving these agents Biosafety Level 3 Practices safety equipment and facilities appropriate for work done with indigenous or exotic agents with a potential for respiratory transmission N A Guide To Biosafety And Biosafety Cabinets which may cause serious and potentially lethal infection More emphasis is placed on primary and secondary barriers to protect
120. ntainment 28 Fluorescent lamps shall be mounted behind the control panel module out of the work zone Electronic ballasts shall be used to eliminate flicker extend lamp life and reduce heat output 29 The UV lamp if installed shall be mounted behind the control panel and away from the users line of site for protection Note Does not apply to 1 8 meter 6 ft model 30 The UV lamp shall operate via an automatic timer with automatic shut off managed by the microprocessor controller and shall be interlocked with the blower motor and fluorescent lights for safety 31 The cabinet shall be designed with a 10 angled front to optimize user comfort reduce glare and maximize reach into the work area 32 The front grille shall be raised to prevent airflow blockage and improve comfort 33 Penetrations for petcocks and service fittings shall be provided penetrations shall be offset to improve user access 34 The cabinet shall accommodate an optional mounting stand for fixed height or adjustable height configurations Control and Alarm System 35 All cabinet functions shall be managed by a programmable microprocessor control system capable of software updates via Internet downloads 36 The microprocessor controller shall be mounted on the main control panel facing down toward the user 37 The controller shall include soft touch keypad controls and backlit LCD displays to permit operation of the blower motor ligh
121. ntrol system provides visual audible alarms for airflow Easy to clean membrane touch control panel is supplied with an LCD display Magnehelic pressure gauge is mounted Esco Airstream Class III biohazard safety cabinets offer the highest level of product operator and environmental protection from infectious biohazardous aerosols and are suitable in the backwall of the workzone for monitoring pressure in the workzone during usage Registered trademark of Dwyer Instruments Inc CONSTRUCTION FEATURES State of the art Neoprene synthetic arm length gloves flame and abrasion resistant cleaner and more resistant to chemicals and flames than regular latex gloves each glove is individually tested for airholes Glove ports are designed to make glove changing easy and most importantly absolutely safe Gloves available in sizes 6 and 7 Integrated pass through with interlocking doors at side of main chamber allows materials to be transferred into the cabinet without the risk of environmental contamination A sloped cabinet front ensures an ergonomic working posture for increased operator productivity and reduces glare from the glass surface Durable and easy to clean stainless steel work surface will never rust or cause contamination Built in warm white electronically ballasted lighting is comfortable to the eyes and offers excellent illumination throughout the work zone in order to reduce operator fatigue
122. nventional decontamination using formaldehyde CH20 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has pungent smell e HPV biological efficacy is independent of environmental variables whereas formaldehyde efficacy is dependent on such variables The Performance Envelope Concept If there is a gap on the cabinet sealing escaping HPV to the lab will decompose to become oxygen and water Escaping formaldehyde however is harmful to people in the lab Therefore HPV decontamination can be performed while people still working inside the lab while formaldehyde decontamination must be performed with no one present in the lab The HPV method improves Safety productivity and reduces the time to seal the cabinet HPV has a better penetration capacity resulting in a full decontamination of the cabinet The formaldehyde method is known to result in incomplete decontamination HPV is more effective and rapid against biological organisms compared to formaldehyde HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hours needed to complete a formaldehyde decontamination process HPV decontamination effectiveness is Independent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 65 For information on the BIOQUELL HPV methodology contact Esco or your Sales Representati
123. o NSF ANSI 49 2004 EN12469 2000 and JIS K3800 2005 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all testing work using harmless Bacillus atrophaeus formerly Bacillus Subtilis bacteria that is used to challenge the cabinet then incubated All exterior painted surfaces are powder coated with Esco Isocide an antimicrobial inhibitor to minimize contamination Isocide is integrated into the coating substrate and cannot wash out or diminish by repeated cleaning Performance results are available upon request Contact Esco or your Esco Sales Representative for details ESCO WORLD CLASS WORLDWIDE P ULPA filtered air _ Unfiltered potentially contaminated air _ Room air Inflow air Cabinet Filtration System Duo Stainless Steel Side Walls Side capture zones Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum blower plenum at the top of the cabinet Dual blowers and dual exhaust filters provide an added measure of protection If the primary blower fails the secondary blower still pushes the air across the exhaust filters to maintain inflow and cont
124. ocol m Automatic control of the entire decontamination cycle No manual intervention required for the entire process m In case of power failure the vaporizer will recover automatically once the power resumes without any intervention m Microprocessor unit has a built in watchdog and will self reset in case of any processor failure m Pre delay contact time neutralisation timers are adjustable using the touch control pad on the front of the unit m Large backlit LCD display m Audible and visual alarms for in case of overheating during a cycle m Password control to prevent decontamination timers from being adjusted by unauthorised personnel m Easy to clean touch control panel without any switches or manual relays to corrode Ordering m Esco Formalin Vaporizer is available with the following electrical configurations Please ensure that the ordering code corresponds to your electrical configuration requirements when ordering Power Supply Options Ordering Codes 220 240VAC 50HZ 60HZ 1 phase pues 100 130VAC 50HZ 60HZ 1 phase r 002 Esco Catalogue Revision No FV v1 10 2004 Specifications subject to change without notice 2004 Esco Micro Pte Ltd ESCO Esco Biotechnology Equipment Division Esco Biotech is a highly focused manufacturer of laminar flow biohazard safety and other HEPA filtered cabinets for the laboratory with a history of quality cabinets since 1978 We are pre
125. of the air in the common plenum is exhausted through the ULPA filter to the room The remain ing 65 of the air is passed through the downflow ULPA filter and into the work area as a vertical laminar flow air stream bathing the work surface in clean air m The uniform non turbulent air stream protects against cross contamination within and throughout the work area e Dual permanently lubricated direct drive external rotor motor blowers safety in the event of a motor failure e The external rotor motor design allows for optimum cooling of the motor during extended operations and ex tends the motor bearing life e The inflow and downflow balance is precisely established by two indepen dent Accuflow fan speed controls e The Accuflow microprocessor based speed controller maintains constant stable airflow despite building supply voltage fluctuations e Speed can be adjusted electronically without mechanical adjustment e Built in RFI and electrical noise filters eliminate interference with adjacent instrumentation e An integral blower hour meter tracks Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right NEW FOR 2007 Infinity Biological Safety Cabinets e Class II Microbiological Safety Cabinet Esco Infinity cabinets use a combination of high performance scroll blowers supply and German made ebm papst permanently lubricated centrifugal motor bl
126. ological Safety Cabinets The uniform non turbulent air stream protects against cross contamination within and throughout the work area Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone The downflow combined with the inflow air enters the common air plenum All air in the common plenum is HEPA filtered and exhausted via a dedicated ducting em to the external environment e The control panel is located on the center of the cabinet and angled down for easy access by the operator e Continuous monitoring of cabinet airflow is displayed on a bright easy to read LCD panel e An integrated temperature compen sated true airflow velocity sensor provides an accurate airflow reading despite room temperature fluctuation e All electronic parts are contained inside a plug and play module that permits easy exchange if required e Microprocessor software updates are available from Esco for download via the Internet Sentinel functions are factory set to default to ON or OFF dependi
127. ontaminated areas across the work surface The autoclavable biohazard collection bag and pipette collection tray should not be placed outside the cabinet The frequent in and out movement needed to use these containers is disruptive to the integrity of the cabinet s air barrier and can compromise both personnel and product protection 4 Operation and Maintenance Most BSCs are designed to permit operation 24 h day and investigators find that continuous operation helps to control the levels of dust and particulate materials in the laboratory Class IIA1 and IIA2 BSCs exhausting to the room or connected by thimble connections to dedicated exhaust ducts can be turned off when not in use Other types such as IIB1 and IIB2 BSCs which have hard duct installations must have air flow through them at all times to help maintain room air balance Cabinets should be turned on at least 5 min before beginning work and after completion of work to allow the cabinet to purge i e to allow time for contaminated air to be removed from the cabinet environment All repairs made on BSCs should be made by a qualified technician Any malfunction in the operation of the BSC should be reported and repaired before the BSC is used again 5 Ultraviolet Lights Ultraviolet lights are not required in BSCs If they are used they must be cleaned weekly to remove any dust and dirt that may block the germicidal effectiveness of the light Ultraviolet light inten
128. oratory grade construction meets Class 100 cleanliness alcohol resistant PVC materials Adjustable 395 490 mm 15 6 19 3 service fixtures to minimize obstructions gas spring struts providing front service access required to direct spills to the drain Filtration IEST RP CC 034 1 USA IEST RP CC007 1 USA IEST RP CC 001 3 USA EN 1822 Europe Electrical Safety IEC 61010 1 Worldwide EN 61010 1 Europe UL 61010A 1 USA CSA C22 2 No 1010 1 92 Canada Germicidal UV lamp Controlled by automatic UV lamp timer through Sentinel microprocessor control panel Emission of 253 7 nanometers for most efficient decontamination Lamp is positioned away from operator line of sight for safety and proper exposure to interior surfaces Note UV lamp intensity reduces over time and its effectiveness is subject to factors such as relative humidity in the cabinet ambient air temperature and microbial species in the work zone Ergonomic foot rest Angled helps maintain proper posture Adjustable height Anti skid coating chemical resistant finish IV Bar with hooks Stainless steel construction Available for all standard cabinets Microscope viewing device Mounting and viewing pouch integrated into sash Factory installed specify when ordering Robust Cabinet Construction and Enhanced Safety Features Service fixtures are offset for easier reach Standard cabinets include two fixtur
129. ork surface 711 mm 28 Typical Penetration A graphical interface indicates cabinet performance Display language can be user programmed for English German French Italian or Spanish Indicators for filter life and UV life are reverse scaled from 100 to 0 and displayed on the LCD panel Enlarged multi line digital read out with alpha numeric display indicates all input status and alarm functions When programmed ON e the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities Containment and Protection The Esco Infinity Class Il Microbiological safety cabinet FC2 provides operator product and environmental protection against Biosafety Levels 1 2 and 3 This cabinet can be used for handling Biosafety Level 4 provided that the operator wears positive pressure sult e The airflow ratio of 65 recirculation to 35 exhaust increases operator pro tection beyond the 70 30 ratio of conventional microbiological safety cabinets e Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination e Raised armrest prevents the likelihood of inflow grille blocking by operator s arms Esco Filter Efficiency Independent supply and exhau
130. outside the workzone to capture B atrophaeus spores that may escape then the sample is incubated e Acceptance The number of B atrophaeus CFU recovered from the agar plates shall not exceed 10 CFU per test Product Protection Test The test objective is to determine cabinet protection to the product samples inside the cabinet workzone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B atrophaeus is placed at 10 cm 4 inches in front of sash window e Target agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus atrophaeus CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet protection from cross contamination of samples placed simultaneously inside the workzone e A nebulizer containing 55 mL of spores 5 to 8 x 10 mL is placed against one of the workzone sidewalls e Target agar plates are placed 36 cm 14 away from the same side wall as the nebulizer e Acceptance The number of Bacillus atrophaeus CFU recovered on agar plates shall not exceed 2 CFU per test HPV Test Compliant Safer Hydrogen Peroxide Decontamination Compatibility Esco biological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decontaminatable cabinets approved by BIOQUELL for this patented process HPV is a safer and more efficient alternative to co
131. owers with external rotor designs exhaust Selected for energy efficiency compact design and flat profile the completely integrated exhaust blower assembly optimizes motor cooling with unified rotating parts and overall component balance for smooth quiet vibration free operation Weight is equally distributed to all bearings to extend bearing life transfer heat and maximize speed control m Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remain der moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that pre vents contaminated room air from enter ing the work zone and prevents work surface emissions from escaping the work zone m Air returns to the common air plenum where the 35 exhaust and 65 recircu lation process is continued operating life and aids in predictive main tenance planning e To prevent fan damage a paper catch grille traps papers or towels that may drop down on the drain pan preventing them from being pulled into the column by fan suction Cabinet Construction Robust construction and enhanced safety features qualify the cabinet for the most demanding laboratory applications The cabinet is fully assembled and ready to install an
132. pean Standard EN 12469 to validate operator personnel protection The retention efficiency for the front aperture shall be not less than 99 999 Microbiological testing for cabinet performance shall be performed on a Statistical sampling basis 4 Each cabinet shall be listed by Underwriters Laboratories UL CUL or CE for electrical safety 5 Original documentation specific to each cabinet serial number shall be provided with the cabinet and maintained in the manufacturers records Test data verifying all performance criteria shall be available upon request to include a inflow velocity through direct inflow measurement method b downflow velocity and uniformity c filter leak scan with aerosol challenge for both filters d light noise vibration e and electrical safety Labculture Filtration System 6 The cabinet shall have one supply downflow filter and one exhaust filter Supply filter shall be ULPA type per IEST RP CC001 3 and meet EN1822 H14 requirements Exhaust filter shall be HEPA type per IEST RP CC001 3 and meet EN 1822 H13 requirements The filters shall be within an aluminum frame with mini pleat design without aluminum separators no wood or fiberboard shall be used in the filter assembly Typical ULPA filter efficiency shall be gt 99 9997 at MPPS and 99 999 for 0 1 to 0 3 microns Typical HEPA Filter shall be gt 99 99 at 0 3 microns An integral filter guard shall be affixed to
133. q e OOP ME NEIRA oa all painted surfaces minimizes contamination Downflow air sensor mounted in interior Each cabinet is KI Discus tested for performance integrity Available in 0 9 1 2 1 5 and 1 8 meter models 3 4 5 and 6 Shown with optional telescoping stand xm ruvNonp GP Tested to EN 12469 and TUV GS certified for safety and performance NEW FOR 2007 Infinity Biological Safety Cabinets Class II Microbiological Safety Cabinet eninge erent With pai vom adjacent equipment Low noise level lt 58dBA 1 2 meter 4 cabinet is significantly quieter than con ventional cabinets Dual long life ULPA per IEST RP CC001 3 filters for supply and exhaust airflow Enhanced side capture zones optimize containment Improved lighting is brighter more uniform and reduces glare Optional UV lamp operates on programmable timer HPV compliant and approved for safe decontamination using BIOQUELL technology The front sash is motorized for conven ient one hand operation The sash control is mounted on the front control panel Integrated sash proximity contacts sense proper sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned When fully lowered the sash seals auto matically against a closed cell peripheral gasket to isolate the interior and prevent escape of contaminants during decontamination x Motorized
134. r IEST RP CC001 3 USA gt 99 995 at MPPS as per EN 1822 H 14 EU oae j Ea NSF ANSI 49 lt 65 5 dBA lt 66 dBA Typical EN 12469 Fluorescent Light Intensity At Zero Ambient Main Body lt 62 5 dBA lt 63 dBA gt 1200 Lux gt 111 5 foot candles gt 1020 Lux gt 95 foot candles 1 5 mm 0 06 16 gauge electrogalvanized steel with white oven baked epoxy Isocide antimicrobial powder coated finish Cabinet Construction Work Zone 1 2 mm 0 06 16 gauge stainless steel type 304 with 4B finish Side Walls 1 2 mm 0 05 18 gauge stainless steel type 304 220 240V AC 50Hz 12 AC2 4D1 AC2 6D1 Electrical 110 130V AC 60Hz 12 AC2 3D2 AC2 6D2 220 240V AC 60Hz 12 Net Weight Shipping Weight Shipping Dimensions Maximum W x D x H Shipping Volume Maximum AC2 3D3 AC2 6D3 223 5 kg 492 7 lbs 305 kg 672 Ibs 245 5 kg 541 2 Ibs 354 kg 780 Ibs 1500 x 950 x 1880 mm 59 x 37 4 x 74 2100 x 950 x 1880 mm 82 7 x 37 4 x 74 2 68 m 96 4 cu ft Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand 3 75 m 132 cu ft Comprehensive Performance Testing At Esco Every Airstream model manufactured by Esco is individually tested documented by serial number and validated with the following test methods Inflow downflow v
135. r and environmental protection e ULPA supply filter per IEST RP CC001 3 is tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron particles providing better filtration capability than conventional HEPA filters that have a typical efficiency of gt 99 99 for 0 3 micron particles e HEPA exhaust filter per IEST RP CC001 3 is tested to a typical efficiency of gt 99 99 for 0 3 micron particles Mini pleat Separatorless Filter left vs Conventional Aluminium Separator Filter right Esco cabinets use Swedish Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the chance of leakage and to prolong filter life Filters include a lightweight aluminum frame for structural stability and elimination of swelling common to conventional wood frames Esco ULPA Filter Efficiency Typical Penetration e Modern separator less mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction e The filter frame and media is constructed in accordance with EN1822 requirements for fire retardant properties e The supply ULPA filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection e The exhaust HEPA filter traps biohazard particles a
136. r control panel Adjustable 395 490 mm 15 6 19 3 When lowered permits movement 7 Emission of 253 7 nanomerers for Ergonomic foot rest through standard doorway most efficient decontamination Angled helps maintain proper Note Increases exterior dimensions Lamp is positioned away from posture operator line of sight for safety and Adjustable height proper exposure to interior surfaces Anti skid coating chemical Note UV lamp intensity reduces over resistant finish time and its effectiveness is subject to e Electrical outlet Europe Worldwide factors such as relative humidity in the e IV bar with hooks Stainless steel construction Electrical Outlets and Utility Fixtures e Electrical outlet ground fault North America e Petcock air gas vacuum cabinet ambient air temperature and North America American style microbial species in the work zone Avallable for all standard Esco cabinets Europe Worldwide style DIN 12898 e PVC armrest Microscope viewing device DIN 12919 DIN 3537 Chemically treated improves operator Mounting and viewing pouch comfort easy to clean 712 mm integrated into sash Factory installed 28 standard size specify when ordering Labculture Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets General Specifications LA2 3A1 LA2 4A1 LA2 5A1 LA2 6A1 International Models LA2 3A3 LA2 4A3 LA2 5A3 LA2 6A3 Europe Asia Pacific Africa x
137. rd r Certifiec Cabinet at least annually ESCO When programmed ON e the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities Electrical Safety and Certification All components meet or exceed applicable safety requirements e Each cabinet is individually factory tested for electrical safety e Documentation specific to each cabinet serial number is maintained on file e Certified to major world standards for microbiological safety cabinets including the EN12469 refer to technical specifications for the full list e Contact Esco or your Sales Representative for site preparation information see Electrical Specifications Warranty Airstream AC2 Series cabinets are war ranted for 3 years excluding consumable parts and accessories e Each cabinet is shipped with a com prehensive User s Manual complete with a report documenting all test procedures e Additional IQ OQ PQ documentation is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests Accessories and Options Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Represen tative for ordering information Support Stands e Fixed height available 737 mm
138. re safety to the user and the environment True airflow velocity both for downflow and inflow velocity sensing technology with temperature compensation for im proved sensor accuracy m Audible and visual alarms for low airflow unsafe sash positions m Automatic warm up cycle is enabled upon turning on the cabinet ensuring all contaminants are purged from the cabinet workzone before the operator can use the cabinet All menu functions are inactive during this period Automatic post purge Independently type tested by Health Protection Agency Porton Down UK to the EN 12469 European Standard for Class II biosafety cabinets Esco Airstream Class II biosafety cabinets are now available in compact 2ft widths designed for laboratories with space constraints This model features many key innovations for which Esco is recognized for mini pleat separatorless ULPA filter technology the best product protection in the world microprocessor based control system external rotor motors superior filter mechanical construction Esco Airstream Class II biosafety cabinets are equipped with ULPA filters typical efficiency of 99 9998 at 0 3 microns that offer a significantly higher efficiency than conventional HEPA filters 99 99 at 0 3 microns used by most cycle is also enabled at shutdown ensuring all residue contaminants are purged out of the workzone before deactivation An admin PIN can be set to restrict unau
139. re used Dual long life ULPA filters for supply and exhaust per IEST RP CC001 3 with 99 999 efficiency for particle size between 0 1 to 0 3 microns m Angled supply filter matches cabinet profile to achieve best downflow uniformity Esco Sentinel microprocessor Frameless shatterproof sash is easier to clean Ergonomically angled front improves reach and comfort m Actual work access height is 45 mm 1 8 higher than tested sash opening Removable one piece work surface simplifies cleaning Raised airflow grille maintains safety by preventing blockage m Optional UV lamp islocated behind control panel away from line of sight Does not apply to 1 8 m 6 models Esco ISS lt TiSE antimicrobial coating on all painted surfaces minimizes contamination Enhanced side capture zones optimize containment Work area on Esco 0 9 meter 3 cabinet is equivalent to work area offered on larger 1 2 meter 4 conventional cabinet Each cabinet is KI Discus tested for performance integrity Available in 0 9 1 2 1 5 and 1 8 meter models 3 4 5 and 6 9 G Fo EN H Uy Shown with optional telescoping stand Oy nN S A NSF 49 UL 61010 JIS K3800 SFDA YY 0569 EN 12469 SANS 12469 Esco Labculture has passed more performance tests in more languages for more certifications throughout more countries than any other biological safety cabinet in the world Labculture Biological S
140. rldwide EN 1822 Europe For Electrical Safety IEC 61010 1 Worldwide EN 61010 1 Europe UL 61010A 1 USA CAN CSA C22 2 No 61010 1 Canada For details on most recent certifications contact Esco or your Esco Sales Representative Airstream Biological Safety Cabinet e Class II Microbiological Safety Cabinets E Series S Series and D Series General Specifications Airstream Class Il Biological Safety Cabinets D Series Note to customer Insert electrical voltage number into last model number digits _ when ordering Model Nominal Size External Dimensions WxDxH Internal Work Area Dimensions WxDxH Internal Work Area Space PS pi ntlow AC2 4D_ 1 2 meters 4 1340 x 740 x 1475 mm 52 8 x29 1 x 58 AC2 6D _ 1 8 meters 6 1950 x 740 x 1360 mm 76 7 x29 1 x 52 9 1270 x 560 x 670 mm 50 x 22 0 x 26 4 1880 x 560 x 670 mm 74 0 x 22 0 x 26 4 0 58 m sq ft 0 87 m 9 3 sq ft 0 45 m s 90 fom at initial setpoint audible visual alarm will activate at 0 40 m s 80 fpm Velocity Downflow 0 30 m s 60 fpm at initial setpoint with uniformity of better than 20 Inflow 270 m h 159 cfm 527 m h 310 cfm iade Ae Vr Downflow 563 m h 331 cfm 1096 m h 645 cfm Exhaust 270 m h 159 cfm 527 m h 310 cfm ULPA Filter Downflow Typical Efficiency Exhaust gt 99 999 at 0 1 to 0 3 microns as pe
141. rocedures e g autoclave or BFI Place contaminated reusable items in biohazard bags autoclavable pans with lids or wrap in newspaper before autoclaving and cleanup Expose non autoclavable materials to disinfectant 20 minute contact time before removal from the BSC Remove protective clothing used during cleanup and place in a biohazard bag for autoclaving Run cabinet 10 minutes after cleanup before resuming work or turning cabinet off gt et o gt B Inside the lab outside the BSC Call the biosafety office if the material is BSL 2 or greater containment Clear area of all personnel Wait at least 15 minutes for aerosol to settle before entering spill area Remove any contaminated clothing and place in biohazard bag to be autoclaved Put on a disposable gown safety glasses and gloves Initiate cleanup with disinfectant as follows 1 Place dry paper towel on spill to absorb liquids then layer a second set of disinfectant soaked paper towels over the spill 2 Encircle the spill with additional disinfectant being careful to minimize aerosolization while assuring adequate contact 3 Decontaminate all items within spill the area 4 Allow 20 minutes contact time to ensure germicidal action of disinfectant Wipe equipment with appropriate disinfectant Discard contaminated disposable materials using appropriate biohazardous waste disposal procedures e g autoclave or BFI 7 Disinfect reusab
142. roper sash position for containment Fluorescent lamps shall be mounted behind the control panel module out of the work zone Electronic ballasts shall be used to eliminate flicker extend lamp life and reduce heat output The UV lamp if installed shall be mounted behind the control panel and away from the user s line of site for protection Note Does not apply to 1 8 meter 6 ft model The UV lamp shall operate via an automatic timer with automatic shut off managed by the microprocessor controller and shall be interlocked with the blower motor and fluorescent lights for safety The cabinet shall be designed with a 10 angled front to optimize user comfort reduce glare and maximize reach into the work area The front grille shall be raised to prevent airflow blockage and improve comfort Penetrations for petcocks and service fittings Shall be provided penetrations shall be offset to improve user access The cabinet shall accommodate an optional mounting stand for fixed height Control and Alarm System 34 35 36 37 38 39 40 41 42 43 All cabinet functions shall be managed by a programmable microprocessor control system capable of software updates via Internet downloads The microprocessor controller shall be mounted on the main control panel facing down toward the user The controller shall include soft touch keypad controls and backlit LCD displays to permit operation of the
143. roprocessor based control system supervises operation of all cabinet functions e The control panel is located on the center of the cabinet and angled down for easy access by the operator e Continuous monitoring of cabinet airflow is displayed on a bright easy to read LCD panel e An integrated temperature compen sated true airflow velocity sensor provides an accurate airflow reading despite room temperature fluctuation e All electronic parts are contained inside a plug and play module that permits easy exchange if required e Microprocessor software updates are available from Esco for download via the Internet Sentinel functions are factory set to default to ON or OFF depending on worldwide destination and local preferences Default settings can be user activated through the touchpad data entry access e Automatic start up sequence will prepare the cabinet for normal operation and advise when safe conditions are established e An administrator controlled PIN Personal Identification Number can be set to restrict access to Main menu Touchpad data entry buttons permit control settings and access to diagnostics default settings and hierarchical menus Programmable automatic UV light timer simplifies operation enhances contamination control extends UV lamp life and saves energy A graphical interface indicates cabinet performance Digital read out with alpha numeric display indicates all input
144. s m Superior electrical safety for the operator all electrical components are UL listed or UL recognized all cabinets are factory tested for electrical safety after production at manufacturing site Designed to meet the general safety requirements of the IEC 61010 1 EN 61010 1 UL61010A 1 CSA C22 2 No 1010 1 92 m Cabinet is shipped fully assembled simply plug the unit into a power source for operation no local installation is required unit is supplied with a 3 pin plug 10 international plug types are available specify order code when ordering Esco Catalogue Revision No AB2 v2 03 2005 Specifications subject to change without notice 2005 Esco Micro Pte Ltd Technical Specifications and Engineering Diagram 305 mm x 12 0 x 1460 mm 57 5 1360 mm 53 5 670 mm 26 4 710 mm 28 0 1 Exhaust HEPA Filter 2 Blower 3 Downflow ULPA Filter 4 Fluorescent Lamp 5 IV Bar Retrofit Kit Provision 6 UV Light Retrofit Kit Provision 7 Service Fixture Retrofit Kit Provisions 2 on each sidewall 8 Internal Single Piece Stainless Steel Work Zone 9 Electrical Electronics Module 10 Esco Sentinel Microprocessor Control 11 Tempered Glass Sash Window 12 Electrical Outlet Retrofit Kit Provisions 2 no s 13 Single Piece Stainless Steel Work Tray 14 Pre Filter 1270 1880 mm 50 0 74 0 1340 1950 mm 52 8 76 8 Genera
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146. s also the most common safety cabinet of all the different types available It has a common plenum from which 30 of air is exhausted and 70 re circulated to the work area as the downflow Type A cabinets exhaust air directly back to the laboratory and they may contain positive pressure contaminated plenums When toxic chemicals must be employed as an adjunct to microbiological processes these cabinets should not be used Exhaust HEPA filtration only removes airborne aerosols including biohazards and not chemical fumes The Class II Type Al has the positively pressurized contaminated plenum bordering the ambient environment and therefore is less safe than the Class II Type A2 that has a negative pressure surrounding the positively pressurized contaminated plenum In case there is a leakage on the positive plenum the leaking aerosol will be pulled by the negative pressure back to the positive plenum and it wont leak outside Because of the safety issue the Type Al design is now considered obsolete In the A2 cabinet about 70 of air from the positive plenum is re circulated as downflow and the remaining 30 is discharaed to the lab throuah the exhaust filter Fig 2 Class II Type A2 BSC Class II Type B Biohazard Safety Cabinets The main difference between Type A and Type B cabinet is Type B cabinets must be operated with an external blower and it exhausts air to the external environment via a dedicated ductwork system Without the
147. s an adjunct to microbiological processes should only be employed if they do not interfere with work when re circulated in the downflow Class II Type B2 Biohazard Safety Cabinet In the Class II Type B2 cabinet all inflow and downflow air is exhausted after HEPA filtration to the external environment without recirculation within the cabinet Type B2 cabinets are suitable for work with toxic chemicals employed as an adjunct to microbiological processes under all circumstances since no re circulation occurs In theory Type B2 cabinets may be considered to be the safest of all Class II biohazard safety cabinets since the total exhaust feature acts as a fail safe in the event that the downflow and or exhaust HEPA filtration systems cease to function normally Fig 3 Class II Type B2 A Guide To Biosafety And Biosafety Cabinets Class III Biohazard Safety Cabinet The Class III biohazard safety cabinet provides an absolute level of safety which cannot be attained with Class I and Class II cabinets All Class III cabinets are usually of welded metal construction and are designed to be gastight Work is performed through glove ports in the front of the cabinet During routine operation negative pressure relative to the ambient environment is maintained within the cabinet This provides an additional fail safe mechanism in case physical containment is compromised On all Class III cabinets a supply of HEPA filtered air provides product prote
148. s needed to complete a formaldehyde decontamina tion process e HPV decontamination effectiveness Is in dependent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 65 e For information on the BIOQUELL HPV methodology contact Esco or your Sales Representative KI Discus Containment Test According to EN 12469 2000 Operator Protection Esco is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is de fined in the European Standard for microbio logical safety cabinets EN12469 2000 as a test method for validating the operator personnel protection capabilities of the cabinet e The Kl Discus test shows excellent correla tion with the microbiological test method for operator protection and is useful for validating the actual containment perfor mance of the cabinet on site e The KI Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus each Esco Infinity FC2 model is fac tory tested using the KI Discus method for operator safety Esco builds quality from the inside out 11 Comprehensive Performance Testing At Esco Every Infinity model manufactured by Esco is individually tested documented by serial number and validated with the following test methods Inflow downflow velocity PAO Aerosol challenge for filter integrity Light noise an
149. sed thickness of lead shielding standard is 3mm Technical Specifications and Engineering Diagram Esco Catalogue Revision LS2 v2 7 2006 Specifications subject to change without notice 2006 Esco Micro Pte Ltd 2190 mm 86 2 670 mm 26 43 760 mm 30 0 KNEE SPACE DEPTH 245 mm 9 6 I 1420 2030 mm I 750 mm 29 5 60 80 780 mm 30 7 1 Exhaust ULPA Filter 2 Blower 3 Downflow ULPA Filter 4 Standard UV Light Retrofit Kit Provision 5 Standard IV Bar Retrofit Kit Provision 6 Electrical Outlet Retrofit Kit Provision Two Single Outlets in Workzone 7 Electrical and Electronics Panel 8 Fluorescent Light 9 Plugged Service Fixture Provisions 2 on each side 10 Stainless Steel Multi piece Work Tray 11 Stainless Steel Armrest 12 KI Impregnated Carbon Filter 13 Esco Sentinel Microprocessor Control System 14 Lead Shielded Glass Sliding Sash Window 15 Stainless Steel Back Wall 16 Lead Shielded Side Walls 17 Horizontal Lead Glass Sliding Door General Specifications LS2 4AX LS2 6AX External Dimensions L x W x H 1420 x 780 x 2190 mm 55 9 x 30 7 x 86 2 2030 x 780 x 2190 mm 79 9 x 30 7 x 86 2 Internal Work Zone L x W x H 1260 x 603 x 670 mm 49 6 x 21 3 x 26 4 1870 x 603 x 670 mm 73 6 x 21 3 x 26 4 Designed to comply and exceed the requirements of DIN12980 La
150. sity should be checked when the cabinet is recertified to ensure that light emission is appropriate Ultraviolet lights must be turned off while the room is occupied to protect eyes and skin from inadvertent exposure 6 Open Flames Open flames should be avoided in the near microbe free environment created inside the BSC They disrupt the air flow patterns and can be dangerous when volatile flammable substances are also used To sterilize bacteriological 13 A Guide To Biosafety And Biosafety Cabinets loops micro burners or electric furnaces are available and are preferable to open flames 7 Spills A copy of the laboratory s protocol for handling spills should be posted read and understood by everyone who uses the laboratory When a spill of biohazardous material occurs within a BSC cleanup should begin immediately while the cabinet continues to operate An effective disinfectant should be used and applied in a manner that minimizes the generation of aerosols All materials that come into contact with the spilled agent should be disinfected and or autoclaved 8 Annual Certification The functional operation and integrity of each BSC should be certified to national or international performance standards at the time of installation and regularly thereafter by qualified technicians according to the manufacturer s instructions Evaluation of the effectiveness of cabinet containment should include tests for cabinet integri
151. st filters provide 99 999 typical efficiency for particle sizes of 0 1 to 0 3 microns 0 0002 A Infinity Series filters meet the IEST RP CC001 3 recommended practice for 0 0 05 0 10 0 15 0 20 0 25 0 30 0 40 Particle Size um Typical Penetration NEW FOR 2007 Infinity Biological Safety Cabinets e Class II Microbiological Safety Cabinet ULPA performance USA and EN 1822 for HEPA performance EU 0 50 e Auto purge holes located at the front side walls eliminate eddy currents and dead air pockets in the critical area behind the sash window e The downflow supply filter is tilted proportional to the cabinet front angle to direct more air forward to the front air grille e The inflow velocity downflow velocity and airflow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front aperture this curtain maintains per sonal and product protection even in the unlikely event of a severe inflow or downflow imbalance that would compromise protection in a conven tional cabinet Integrated Filtration System Independent supply and exhaust filters provide 99 999 typical efficiency for particle sizes of 0 1 to 0 3 microns Infinity Series filters meet the IEST RP CC001 3 recommended practice for ULPA per formance USA and EN 1822 for H14 performance EU e ULPA filters per IEST RP CC001 3
152. st recent certifications contact Esco or your Sales Representative Accessories and Options Esco offers a variety of options and acces sories to meet local applications Contact Esco or your local Sales Representative for ordering information Perforated Work Tray Available for applications where a per forated work surface is preferred Must be specitied when ordering Electrical Outlets and Utility Fittings e Electrical outlet ground fault North America e Electrical outlet Euro Worldwide e Petcock air gas vacuum North America American style Euro Worldwide style DIN 12898 DIN 12919 DIN 3537 Support Stands e Fixed height available 737 mm 29 or 838 mm 33 38 1 mm 1 5 With leveling feet With casters NEW FOR 2007 Infinity Biological Safety Cabinets e Class II Microbiological Safety Cabinet e Adjustable height hydraulic range from 737 mm 29 to 838 mm 33 Manual or electrical lift With casters e Telescoping height nominal range from 737 mm 29 to 838 mm 33 Adjustable in 25 4 mm 1 increments e Infinitely adjustable cradle stand with casters Elevates to seating or standing work surface height When lowered permits movement through standard doorway Note Increases exterior dimensions Cabinet Accessories e PVC armrest Chemically treated improves operator comfort easy to clean 712 mm 28 standard size e Ergonomic lab chair Lab
153. t UV lamp electrical outlet s and menu 38 The controller shall be user programmable in situ to enable or disable functions such as PIN personal identification number access restriction cabinet start up protocol airflow alarm and other microprocessor controlled operations outlined in the user manual 39 When programmed ON the start up protocol shall perform an automatic pre purge and post purge cycle to ensure proper cabinet operation 40 The controller shall include a blower motor hours meter to display aggregate motor running time to assist in predictive maintenance 41 Audible and visual alarms shall be provided for unsafe conditions such as improper airflow or sash position 21 Airflow shall be monitored by a temperature compensating thermistor based true air velocity sensor mounted in the cabinet 43 The airflow display and alarm system shall be individually calibrated before shipment 44 The main control panel shall exhibit continuous display of air velocity and a 24 hour clock display Certification Service and Decontamination 45 The cabinet shall be approved for both hydrogen peroxide vapor HPV and formaldehyde decontamination protocol 46 All panels leading to potentially contaminated and or hazardous areas shall be color coded red 47 All components with the exception of blower motor and ULPA filters shall be located outside of contaminated air spaces to facilitate servicing without the
154. t Kit provision Universal electrical outlet 0 9 meter 3 model one single outlet in work zone 1 2 1 5 and 1 8 meter 4 5 amp 6 models two single outlets in work zone 43 9 55 0 67 9 7 79 9 7 Exhaust fan 8 Electrical Electronics panel 9 Fluorescent light 10 Gas service fixture with solenoid valve 11 Vacuum service fixture 12 Stainless steel single piece work tray 13 Stainless steel armrest 14 Key switch 15 Esco Sentinel microprocessor a control system Access Opening Height 805 mm 31 7 All Model Sizes 16 Motorized sliding sash Testing Opening Height 200 mm 7 9 aerosol tight Working Area Height 210 mm 8 3 17 Single piece stainless steel w back wall and sidewall The combination of the Esco raised armrest and recessed 18 Side removable pane for i than typically specified plumbing access 19 Thimble exhaust collar optional Optional Exhaust Collar Positions for Thimble Ducting for FC2 Models 262 mm 10 3 460 mm 18 1 x Em N S O I 1015 mm 40 0 Side View All Sizes Top View FC2 6A_ work surface creates additional space within the working area ESCO WORLD CLASS WORLDWIDE 10 General Specifications FC2 3A_ Nominal Size 0 9 meters 3 1115 x 805 x 1535 mm 43 9 x 31 7 x 60 4 External Dimensions WxDxH FC2 4A_ 1 2 meters 4 1420 x 805 x 15
155. t ULPA Filter 2 Blower 3 Downflow ULPA Filter 4 UV Light Retrofit Kit Provision 5 IV Bar Retrofit Kit Provision 6 Service Fixture Retrofit Kit Provisions 2 One Each Side 7 Electrical amp Electronics Panel 8 Fluorescent Lamps 9 Tempered Glass Sidewalls 10 Stainless Steel Divided Work Tray 2pcs 11 Stainless Steel Armrest 12 Esco Sentinel Microprocessor Control System 13 Tempered Glass Sliding Sash 14 Electrical Outlet Retrofit Kit Provision 15 Optional Exhaust Collar General Specifications External Dimensions L x W x H AC2 2EX 730 x 732 x 1435 mm 28 7 x 28 8 x 56 5 Internal Work Zone L x W x H 660 x 560 x 670 mm 26 0 x 22 0 x 26 4 Standards Compliance EN 12469 2000 type tested exceeds requirements for Class Il microbiological safety cabinets Air cleanliness ISO 14644 1 Class 3 IEST G CC1001 IEST G CC1002 and other equivalent requirements Filter performance IEST RP CC034 1 IEST RP CC007 1 IEST RP CC001 3 and EN1822 Electrical safety IEC 61010 1 EN 61010 1 UL 3101 1 CSA C22 2 No 1010 1 92 Cleanliness Within Working Area Average Airflow Inflow Initial setpoint 0 45 m s or 90 fom audible visual alarm will activate at 0 4m s or 80fpm Velocities Downflow Initial setpoint 0 31 m s or 61 fpm uniformity is 20 Airflow Volumes Inflow 159 cmh 94 cfm At Initial Airflow Downflow 70 384 cmh 226 cfm Velocity Setpoints Exhaust 30
156. t the need to decontaminate the cabinet All exterior surfaces shall be painted with a permanent antimicrobial inhibitor coating to minimize contamination ECO Airstream Class II Type B2 Biosafety Cabinets MAIN FILTRATION AGENTS m ISO Class 3 air cleanliness within workzone as per ISO 14644 1 equivalent to Class 1 as per the US Federal Standard 209E 100 times cleaner than the usual Class 100 classification on cabinets offered by the competition m Minipleat separatorless H13 HEPA U15 ULPA filter technology operating at the typical efficiency of 99 999 at MPPS 0 3 and 0 12 microns provides better operator product and environmental protection than conventional HEPA filters CABINET CONTROL SYSTEM Esco Sentinel Microprocessor Cabinet Control System allows the user to easily access all cabinet functions The control system monitors and displays cabinet airflow constantly on the control LCD screen prompting the user with audible and visual alarms in case of any unsafe condition m True airflow velocity sensing technol ogy with temperature compensation for improved sensor accuracy m Audible and visual alarms for low airflow unsafe sash positions m Automatic warm up cycle is enabled upon turning on the cabinet ensuring all contaminants are purged from the cabinet workzone before the operator can use the cabinet All menu functions are inactive during this period Automatic post purge cycle can be
157. tages may be available contact Esco for ordering information Cabinet only excludes optional stand 2 58 m 91 cu ft 3 22 m 114 cu ft 3 62 m 128 cu ft 1 AC power specifications are to be specified in the last digit of the model number when ordering 1 220 240V 50Hz 2 110 130V 60Hz 3 220 240V 60Hz Note 60 Hz models meet or exceed NSF 49 standards for safety and performance For information contact Esco or your Sales Representative NEW FOR 2007 Infinity Biological Safety Cabinets e Class II Microbiological Safety Cabinet Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and interna tional criteria Testing in our micro biology laboratory is conducted according to NSF49 EN12469 and JIS K3800 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all testing work using harmless Bacillus atrophaeus for merly Bacillus Subtilis bacteria that is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Units CFU are counted to determine the test ing results Increased microbiological chal lenge tests with objects inside the cabinet work zone Bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test
158. the workzone sidewalls e Target agar plates are placed 36 cm 14 away from the same side wall e Acceptance The number of Bacillus atrophaeus CFU recovered on agar plates shall not exceed 2 CFU per test Esco cabinets are designed to operate within a performance envelope to maintain protection for personnel product and the environment Airflow parameters used to frame the performance envelope include both inflow velocity and downflow velocity The graph illustrates the boundaries of the performance envelope based on a 1 2 meter 4 ft cabinet as well as the nominal performance point at which tests are conducted The range between high and low Inflow and high and low downflow together with the fluid dynamics achieved through sophisticated cabinet design proportionally size capture slots and uniform laminar airflow combine to deliver a complete containment and safety solution expected of a professional biological safety cabinet For details on the Esco performance envelope contact Esco or your Esco Sales Representative 0 50 m s Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets HPV Test Compliant Safer Hydrogen Peroxide Decontamination Compatibility Esco biological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decontaminatable cabinets approved by BIOQUELL for this patented process HPV is asafer and more efficient alternative to conventional decontamina
159. thorized access to all menu functions ERGONOMIC FEATURES Ergonomic sloped front design allows for maximum visibility into the workzone The frameless tempered glass sliding front sash and tempered glass sidewalls provide a greater visibility into the workzone m Built in 5000k fluorescent lighting offers excellent illumination throughout the workzone Electronically ballasted lighting system is instant start non flickering and energy efficient m Cabinet armrest is raised above the workzone to ensure that the operator s arms do not block the front inflow perforations Optional PVC soft armrest padding available upon request Refer to our Accessories catalogue CABINET BODY CONSTRUCTION m All components designed for maximum chemical resistance for long service life and durability All cabinet components are cleanroom compatible m Industrial grade main body constructed of electrogalvanised steel with an abrasion resistant white oven baked powder coated finish Attractive two piece stainless steel work trays are easy to remove for purposes of cleaning and auto claving m Permanently lubricated direct drive centrifugal blower s energy efficient external rotor motor type design reduces other manufacturers operating costs extremely low noise and vibration levels less than 60dBA at working position due to proprietary construction and mounting technology The cabinet s airflow is self regulat
160. tion using formaldehyde CH20 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has pungent smell e lf there is a gap on the cabinet sealing escaping HPV to the lab will decompose to become oxygen and water Escaping formaldehyde however is harmful to people in the lab Therefore HPV decontamination can be performed while people still working inside the lab while formaldehyde decontamination must be performed with no one present in the lab The HPV method improves safety productivity and reduces the time to seal the cabinet e HPV biological efficacy is independent of environmental variables whereas formaldehyde efficacy is dependent on such variables e HPV has a better penetration capacity resulting in a full decontamination of the cabinet The formaldehyde method is known to result in incomplete decontamination e HPV is more effective and rapid against biological organisms compared to formaldehyde e HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hours needed to complete a formaldehyde decontamination process e HPV decontamination effectiveness is independent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 65 e For information on the BIOQUELL HPV methodology contact Esco or your Sales Representative KI Discus Containment Test According to EN
161. to 0 3 microns Airstream Series filters meet the IEST RP CC001 3 ee e s s ele gay recommended practice for ULPA performance USA and EN 1822 for HEPA performance EU 0 0 05 0 10 0 15 0 20 0 25 0 30 Particle Size um Airstream 0 40 0 50 Typical Penetration e A true airflow velocity sensor provide independent measurement of inflow and downflow velocities despite room temperature fluctuation e All electronic parts are contained inside a plug and play module that permits easy exchange if required e Microprocessor software updates are available from Esco for download via the Internet Consult your Esco Operating Manual or contact Esco or your Sales Representative for information on user preference programming capabilities built into the Sentinel microprocessor platform Cabinet Construction Robust construction and enhanced safety features qualify the cabinet for the most demanding laboratory applications The cabinet is fully assembled and ready to install and operate when shipped e The cabinet work zone has no welded joints to collect contaminants or rust e All stainless steel work surfaces are accessible for cleaning e S Series cabinets stainless steel sides include interior sides and back wall formed from a single piece of stainless steel with large radius corners to simplify interior cleaning e E Series
162. ty HEPA filter leaks downflow velocity profile face velocity negative pressure ventilation rate air flow smoke pattern and alarms and interlocks Optional tests for electrical leaks lighting intensity ultraviolet light intensity noise level and vibration may also be conducted Special training skills and equipment are required to perform these tests and it is highly recommended that they are undertaken by a qualified professional 9 Cleaning and Disinfection Allitems within BSCs including equipment should be surface decontaminated and removed from the cabinet when work is completed since residual culture media may provide an opportunity for microbial growth The interior surfaces of BSCs should be decontaminated before and after each use The work surfaces and interior walls should be wiped with a disinfectant that will kill any microorganisms that might be found inside the cabinet At the end of the work day the final surface decontamination should include a wipe down of the work surface the sides back and interior of the glass A solution of bleach or 70 alcohol should be used where effective for target organisms A second wiping with sterile water is needed when a corrosive disinfectant such as bleach is used It is recommended that the cabinet be left running If not it should be run for 5 min in order to purge the atmosphere inside before it is switched off 10 Decontamination BSCs must be decontaminated before filter c
163. uct and process within the work zone from airborne contamination from ambient air c and the product and process within the work zone from cross contamination 3 Before shipment each cabinet shall be individually tested by the KI Discus test European Standard EN12469 2000 to validate operator personnel protection The retention efficiency for the front aperture shall be not less than 99 999 Microbiological testing for cabinet performance shall be performed on a statistical sampling basis 4 Each cabinet shall be listed by Underwriters Laboratories UL CUL or CE for electrical safety 5 Original documentation specific to each cabinet serial number shall be provided with the cabinet and maintained in the manufacturers records Test data verifying all performance criteria shall be available upon request to include a inflow velocity through direct inflow measurement method b downflow velocity and uniformity c filter leak scan with aerosol challenge for both filters d light noise vibration e and electrical safety Filtration System 6 The cabinet shall have one supply downflow filter and one exhaust filter Both filters shall be ULPA type per IEST RP CC001 3 and meet EN1822 H14 requirements 7 The filters shall be within an aluminum frame with mini pleat design without aluminum separators no wood or fiberboard shall be used in the filter assembly 8 Typical filter efficiency shall be 99 9997
164. uirements e Each cabinet is individually tested for electrical safety at factory e Documentation specific to each cabinet serial number is maintained on file e UL Listed for USA and Canada e Certified to all major world standards for biological safety cabinets including the NSF ANSI 49 and EN 12469 refer to Standards Compliance table on this page for the full list e Contact Esco or your Sales Representative for site preparation information see Electrical Specifications 3G lt ivE Antimicrobial Powder Coating Bacterial Population 10 folds 8 hours 0 4 hours 12 hours 16 hours 20 hours 24 hours Contact Time in Hours Labculture Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets Warranty The Labculture cabinet is warranted for 3 years excluding consumable parts and accessories e Each cabinet is shipped with a compre hensive user s manual complete with a report documenting all test procedures e Additional IQ OQ PQ documentation is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests Accessories and Options Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Represen tative for ordering information Support Stands e Fixed height available 711 mm 28 or 864 mm 34 38 1 mm 1 5 With
165. uld be placed as far into the cabinet working space as possible to minimize its effect on cabinet performance 4 A solenoid valve can be interlocked with the gas supply to automatically shut off the burner 4 Using BSCs Shutdown Note operate the cabinet continuously whenever possible to maximize protection Surface decontaminate all items Seal biohazard bags if used Surface decontaminate the cabinet innerside walls back wall work surface drain pan and the inner side of the sliding sash hinged window Allow work zone air to purge When available install front closure or close sash and activate UV lamp if desired 5 User maintenance Pre filter replacements once every 3 months UV lamp replacement once a year Operational verification if appropriate e g Alarm checks basic airflow measurements etc 17 A Guide To Biosafety And Biosafety Cabinets IV Biohazard Spills amp Cleanup Procedures A Inside the BSC Wait at least five minutes to allow the BSC to contain aerosols Wear lab coat safety glasses and gloves during cleanup Allow cabinet to run during cleanup Apply disinfectant and allow a minimum of 20 minutes contact time Wipe up spillage with disposable disinfectant soaked paper towel Wipe the walls work surface and any equipment in the cabinet with a disinfectant soaked paper towel Discard contaminated disposable materials using appropriate biohazardous waste disposal p
166. ve Esco cabinets are designed to operate within a performance envelope to maintain protection for personnel product and the environment Airflow parameters used to frame the performance envelope include both inflow velocity and downflow velocity The graph illustrates the boundaries of the performance envelope as well as the nominal performance point at which tests are conducted The range between high and low Inflow and high and low downflow together with the fluid dynamics achieved through sophisticated cabinet design proportionally size capture slots and uniform laminar 0 65 I s High Inflow High Inflow High Downflow Low Downflow 0 61 0 43 0 60 0 58 0 30 Nominal Inflow 0 55 Nominal Downflow gt 0 53 0 35 q gt gt 0 50 2 r Low Infi Low Inflow 0 45 k al a hi High Downflow 0 48 0 30 0 48 0 40 0 40 0 25 0 30 0 35 0 40 0 45 Downflow velocity airflow combine to deliver a complete containment and safety solution expected of a professional biological safety cabinet For details on the Esco performance envelope 0 590 contact Esco or your Esco Sales Representative m s ESCO WORLD CLASS WORLDWIDE 10 KI Discus Containment Test According to EN 12469 Potassium lodide Esco Is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is defined in the European Standard for microbiolo
167. ving their arms into and out of cabinets Arms should be moved in and out slowly perpendicular to the front opening Manipulations of materials within BSCs should be delayed for about 1 min after placing hands and arms inside to allow the cabinet to adjust and to air sweep the surface of the hands and arms The number of movements across the front opening should also be minimized by placing all necessary items into the cabinet before beginning manipulations BSC Biological Safety Cabinet jiii Return Air Grille Personnel Traffic Barrier Airflow Air Inlet Fig 8 A typical Class II Cabinet Installation 12 A Guide To Biosafety And Biosafety Cabinets 3 Material Placement The front intake grill of Class II BSCs must not be blocked with paper equipment or other items Materials to be placed inside the cabinet should be surface decontaminated with 70 alcohol Work may be performed on disinfectant soaked absorbent towels to capture splatters and splashes All materials should be placed as far back in the cabinet towards the rear edge of the work surface as practical without blocking the rear grill Aerosol generating equipment e g mixers centrifuges etc should be placed towards the rear of the cabinet Bulky items such as biohazard bags discard pipette trays and suction collection flasks should be placed to one side of the interior of the cabinet Active work should flow from clean to c
168. y related instructions beatore use Teast Lae ea fo Are Slain Prolecioe SO 9001 Gualit F Manufacturing Environment EC 61010 1 E ire G rillo this cabinet at least annually a Carin Certified ESCO When programmed ON e the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities The cabinet work zone has no welded joints to collect contaminants or rust All stainless steel work surfaces are accessible for cleaning Tray components lift and remove to provide easy access and encourage surface decontamination e There are no screws in on the front or sides to trap contaminants or complicate cleaning e Optional service fittings are offset for easier access e External plumbing is concealed behind trim panels to maintain aesthetics Robust construction and enhanced safety features qualify the cabinet for the most demanding laboratory applications The cabinet is fully assembled and ready to install and operate when shipped e A recessed central area and stainless External surfaces are coated with steel drain pan channels spills and Esco Isocide antimicrobial coating to prevent liquids from entering the lower protect against surface contamination filtration and blower systems and inhibit bacterial growth Isocide eliminates 99 9 o
169. y removing the sash track cover and swinging up the sash glass The sash is counterbalanced for smooth lightweight operation Esco cabinets use ULPA filters per IEST RP CC001 3 instead of conventional HEPA filters commonly found in biological safety cabinets While HEPA filters offer 99 99 typical efficiency at 0 3 micron level ULPA filters 0 0 05 0 10 0 15 0 20 0 25 0 30 Particle Size um Typical Penetration provide 99 999 typical efficiency for particle sizes of 0 1 to 0 3 micron level 0 40 0 50 ESCO WORLD CLASS WORLDWIDE ULPA filtered air L Unfiltered potentially contaminated air L Room air Inflow air e The counterbalance locking mechanism is inherently safe it locks the counter balance in place if either of the 2 cables is detached The sash cable and cable clip have rated strength of more than 6 times the weight of the sash window of the largest Labculture Class Il cabinet 1 8 meter 6 ft model e The safety glass maintains containment if the sash is accidentally broken during cabinet operation Blower Efficiency The Labculture blower system is designed for high performance operation maximum energy efficiency and minimal maintenance e The external rotor motor design allows for optimum cooling of the motor during extended operations and extends the motor bearing life Cabinet Filtration System Sid
170. zone to ensure that the operator s arms do not block the front inflow perforations CABINET BODY CONSTRUCTION m All components designed for maximum chemical resistance for long service life and durability All cabinet components are cleanroom compatible recirculation within the cabinet These cabinets are supplied with an integral exhaust collar that allows the cabinet exhaust to be ducted to the external environment via a dedicated exhaust system As they incorporate a non recirculating total exhaust design Class II Type B2 cabinets are suitable for work with toxic chemicals when these are present in trace amounts Industrial grade main body constructed of electrogalvanised steel with an abrasion resistant white oven baked powder coated finish Attractive single piece stainless steel work surface is easy to remove m Inherently safe design maintains containment for protection even with work tray removed thus ensuring safety for the operator during cleaning Permanently lubricated direct drive centrifugal blower s energy efficient external rotor motor type design reduces operating costs extremely low noise and vibration levels less than 64dBA at working position due to proprietary construction and mounting technology m Built in solid state variable speed controller s infinitely adjustable from zero to the maximum setting with built in RFI and noise filters is superior to conventional step controller

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