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Leica RM2245

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1. Fig 18 Important Never turn the screw more than 2 turn ata time Leica RM2245 Fine adjustment of the force balance lf another accessory of a different weight Is mounted on the object head 33 you must check whether it is necessary to readjust the force balance Checking the correct setting e Attach the new accessory and clamp the specimen e Set the object head to half the height of the vertical travel range by turning the handwheel Fig 18 lf the object head remains in this exact position the setting Is correct lf the object head moves i e it is raised or low ered fine adjustment Is necessary A The force balance is adjusted using the screw 34 which can be accessed by removing the section waste tray on the bottom of the base plate of the microtome Use the Allen key pro vided size 5 with handle for the adjustment Failure to adjust the force balance may result in injury while working e ftheobjecthead moves downwards turn the screw approx turn clockwise e fthe objecthead moves upwards turn the screw 34 approx turn counterclockwise e Continue this procedure until the object head no longer moves once released 29 5 Operation 5 2 Inserting the knife holder 55 53 Enlarged detail Scale for better repositioning of the knife holder for varying specimen heights Sn uaa
2. Fig 47 Fig 48 52 6 6 Tray The tray is mounted on the hood of the micro tome so that the small feet on the underside fit into the cutouts on the hood It is for storage of the utensils used during sectioning as well as the sectioned specimens 6 7 Freezer pack The freezer pack consists of the freezer plate 98 and the insulation jacket 99 It is for cooling or keeping cold paraffin specimens Up to 35 standard cassettes can be cooled at the same time The specimens require approx 20 min for being cooled from room temperature approx 20 C to sectioning temperature The cooling performance depends on the ambient temperature and the volume of the specimens being cooled The insulation jacket can be placed on the hood of the microtome in the same manner as the tray or it can be placed next to the instrument i e Remove the freezer plate from the insulation jacket and place it in a deep freezer ideally overnight but for at least six hours at approx 23 C e Reassemble the freezer plate and insulation jacket and place them on the bench or the instrument When using the freezer pack on the instrument the tray Fig 44 must be removed Instruction manual V 1 5 11 2008 6 Optional accessories 6 8 Universal microscope carrier Leica RM2245 Unpack all accessories in the package and check for completeness Baseplate with drilling a
3. 58 30 Fig 21 Setting up the knife holder base e Release the clamping lever 50 by rotating it counterclockwise e Insert the knife holder base 51 using the notch 52 on the bottom into the T piece 55 of the microtome base plate 53 e To secure the knife holder base turn the clamping lever 50 clockwise The knife holder base 51 can be moved back and forth on the microtome base plate This allows bringing the knife holder to optimal sectioning position in relation to the specimen There is a scale 54 on the right side of the microtome base plate This enables faster and better positioning of the knife holder at the specimen if various combinations of standard Specimens and specimen holders are used The rear edge of the knife holder base 51 functions as the scale reference Inserting the knife holder e Loosen the screw 58 using an Allen key size 4 71 until the knife holder 57 can be moved e Place the knife holder 57 with the under side groove onto the T piece 56 of the knife holder base 51 e To clamp retighten the screw 58 Instruction manual V 1 5 11 2008 5 Operation 5 3 Inserting the universal cassette clamp Fig 22 There are two versions of the specimen holder one with and one without specimen orientation which are interchangeable The object orientation allows for simple position correction of the specimen surface when the
4. 5 Numbers in parentheses refer to item numbers in illustrations Function keys which must be pressed on the control panel are shown in the text in bold capital letters RUN STOP 1 2 Qualification of personnel e The Leica RM2245 may be operated by trained laboratory personnel only e All laboratory personnel designated to oper ate the Leica instrument must read this instruction manual carefully and must be familiar with all technical features of the instrument before attempting to operate it 1 3 Intended use of instrument The Leica RM2245 is a semi motorized rotation microtome with a separate control panel for creating thin sections of specimens of varying hardness for use in routine and research labo ratories in the fields of biology medicine and industry It is designed for cutting soft paraffin speci mens as well as harder specimens as long as they are suitable for being cut manually The Leica RM2245 is also suitable for IVD in vitro diagnostics Any other use of the instrument is considered improper 1 4 Instrument type All information provided in this manual applies only to the instrument type Indicated on the cover page An identification label indicating the instrument serial number is attached at the left side of the instrument Fotr Nr 0486 10 2005 Kat Nr 050138178 Modell RM 2245 100 120230 240 7 50 60 Hz 70 VA _ Fig 1 Instruction man
5. 46 The clamping levers on the knife holder are not interchangeable The two clamp ing levers 10 11 must remain in the po sition shown at all times as otherwise isolated malfunctions of the knife hold er can occur Clamping lever for the blade 10 at the right clamping lever for the lateral displacement 11 at the left Instruction manual V 1 5 11 2008 6 Optional accessories Knife holder E 10 Fig 41 Note on the knife holder E Lateral displacement only for knife holder E The lateral movement feature of the knife holder base enables the use of the entire length of the blade or knife eliminating the need for re adjusting the knife holder The knife holder E consists of a segment arch A with lever 11 and the clamp mount B with lever 10 and ejector 13 The extreme left and right positions as well as the middle position are each marked with a notch point e To release the clamp rotate the lever 11 on the left side of the knife holder forwards e Move the knife holder sideways e To clamp rotate the lever 11 back The knife holder E is an important precision component the quality and precise adjustment of which have a lasting effect on the entire function of the microtome In case of malfunctions or damages to the clamp mount it is always required to replace the clamp mount including the pertinent clamping lever Leica Biosystems offers spec
6. The motorized object head can be moved at two speeds 300um s and 900um s The coarse drive speed and direction can be controlled via buttons at any time The minimum and maximum object head end positions are signaled visually and acoustically The thickness for trim and sectioning modes can be programmed and stored indepen dently A ruler integrated in the base of the microtome facilitates restoring a previous knife holder position 13 3 Instrument components and specifications 3 3 Technical data General Approvals Nominal supply voltages Nominal frequency Power draw Protective class Power fuses Pollution degree Overvoltage installation Maximum heat emission Operating temperature range Temperature range during storage Relative humidity Humidity during storage The instrument specific marks are located next to the identification label 100 120 230 240 V AC 10 50 60 Hz 70 VA 2 x 11 0 A UL listed 2 II 70 J s 10 C to 35 C 5 C to 55 C max 80 non condensing lt 80 according to IEC 1010 UL 3101 EN 61010 Dimensions and weight Basic instrument Width including handwheel Width excluding handwheel Depth including waste tray Height total Working height knife blade Working height knife blade Weight without accessories Control panel 14 Width Depth Height Height in inclined position Weight
7. To clamp the knife 47 evenly screw the two knife clamping screws 49 inward rotate clockwise Lateral repositioning of the knife 50 Push knife guard 8 to the center Loosen the clamping screws 49 by turning them counterclockwise Push the knife 47 to the left or right as required To clamp the knife 47 always tighten the clamping screw 49 first which is located on the side to which the knife has been repositioned by turning it clockwise Instruction manual V 1 5 11 2008 6 Optional accessories Fig 45 Leica RM2245 6 4 Section waste tray Push the section waste tray 18 from the front to the microtome base plate 53 until it is held in place by the two magnets 39 on the front of the microtome base plate To remove the section waste tray lift it slightly and pull it off towards the back 6 5 Backlighting The backlighting is inserted at the front into the one piece knife holder base Remove the two screws 1 using a slotted screwdriver and then remove the cover plate 2 Insert the backlighting 3 in the recess at the rear of the knife holder base Connect the plug 4 of the backlighting to the socket 5 on the microtome The backlighting illuminates once the micro tome is turned on with the mains switch NEVER use the backlighting illumination with the super mega cassette clamp 51 6 Optional accessories
8. i When using both brake systems at the same time always move the lever 3 to position first Otherwise it may be not be possible to release the lever 5 Instruction manual V 1 5 11 2008 Knife holder N ma Fig 4 Knife holder E 10 9 Leica RM2245 2 Safety Knife guard on the knife holder Each knife holder is equipped with a tightly mounted knife guard 8 9 This makes it possi ble to cover completely the cutting edge in every knife or blade position Knife holder N NZ The knife guard 8 of the knife holder N NZ can be easily positioned via the two handles 7 Fig 4 To cover the knife edge push both cover strips of the knife guard to the center Knife holder E E TC The knife guard on knife holder E E TC consists of a red foldaway handle 9 To cover the cutting edge fold the knife guard handle 9 upwards as illustrated in Fig 5 A The clamping levers on the knife holder E are not interchangeable The two clamping levers 10 11 must remain in the position shown at all times as otherwise isolated malfunctions of the knife holder can occur Clamping lever for the blade 10 at the right clamping lever for the lateral dis placement 11 at the left 11 3 Instrument components and specifications 3 1 Overview instrument components Leica RM2245 Instrument control panel oe Handwheel Directional l
9. CE Instruction Manual Leica RM2245 V1 5 English 11 2008 Always keep this manual with the instrument Read carefully before working with the instrument Leica RM2245 Rotary Microtome etca MICROSYSTEMS NOTE The information numerical data notes and val ue judgments contained in this manual repre sent the current state of scientific knowledge and state of the art technology as we under stand it following thorough investigation in this field We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical develop ments nor to provide our customers with addi tional copies updates etc of this manual For erroneous statements drawings technical illustrations etc contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case In particular no liability what soever is accepted for any financial loss or con sequential damage caused by or related to compliance with statements or other informa tion in this manual Statements drawings illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products Published by Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Phone 49 0 6224 143 0 Fax 49 0 6224 143 200 Inte
10. Universal cassette clamp e Shaft 90 and flat coil spring 91 of the clamping lever of the cassette clamp After lubrication move the lever back and forth several times Instruction manual V 1 5 11 2008 9 Warranty and service Warranty Service information Leica Biosystems Nussloch GmbH guarantees that the contractual prod uct delivered has been subjected to a comprehensive quality control pro cedure based on the Leica in house testing standards and that the prod uct is faultless and complies with all technical specifications and or agreed characteristics warranted The scope of the warranty is based on the content of the concluded agreement The warranty terms of your Leica sales organization or the or ganization from which you have purchased the contractual product shall apply exclusively If you are in need of technical customer service or spare parts please contact your Leica representative or the Leica dealer where you purchased the instrument Please provide the following information e Model name and serial number of the instrument e Location of the instrument and name of the person to contact e Reason for the service call e Delivery date Decommissioning and disposal Leica RM2245 The instrument or parts of the instrument must be disposed of according to existing local applicable regulations Special attention should be paid to the lithium cell of the electronic circuit board 67 10
11. Leica Biosystems Nussloch GmbH Postfach 1120 D 69222 Nussloch May 15 2008 President Biosystems Devision Peoples Republic of China Administrative Measures on the Control of Pollution Caused by Electronic Products BEA MmaAICH Hazardous substances BDA FR Name of the part ED il E oe tR printed circuit boards Fa 70 ee electronic components ELER BB FE mechanical parts HF TC ae FF optical components FE HR light sources o RARZASASVREZGREPHARYESAJ T 11363 2006 R EHRE KAUT Indicates that the concentration of the hazardous substance in all materials in the parts is below the relevant threshold of the SJ T 11363 2006 standard X RRRZABASVREYLEZRAHR HME PHA EE HST 11363 2006 EMEN RBZ Indicates that the concentration of the hazardous substance of at least one of all materials in the parts is above the relevant threshold of the SJ T 11363 2006 standard Note The actual product may or may not include in all the part types listed above Leica RM2245 69 Notice 70 Instruction manual V 1 5 11 2008
12. Vertical column with size 8 cap screw b and lock washer c Horizontal arm with cross member d and support ring e Support plate large for RM2235 RM2245 and RM2255 Support plate small for RM2265 Allen key size 3 4 countersunk screws to install support plate Allen key size 8 Assembling the universal microscope carrier Attach baseplate Select the large 5 or small 4 support plate depending on the microtome to be used Attach the support plate to the baseplate with the included 4 countersunk screws 7 using the Allen key size 3 6 53 6 Optional accessories 2 Attach vertical column e Insert the cap screw b into the hole of the baseplate from below Place the lock washer c on the cap screw from above e Thread the silver vertical column 2 onto the baseplate from above and tighten with the Allen key size 8 The lock washer must be positioned between the baseplate and vertical column to prevent unintentional turning of the column Extreme danger of tipping After installing the vertical column immediately place the microtome onto the baseplate so that the front feet of the microtome are positioned in the shallow recesses A 3 Attach horizontal arm Slide the support ring e onto the vertical column and position it so that the lock nut f is facing to the back Tighten the lock nut e Slide the cross member d onto the column Ensure that the lock screw g is facing to the
13. e Turn the knurled screw 66 clockwise to move the movable jaw with the vee insert upward against the fixed jaw to securely clamp the sample 39 6 Optional accessories 6 2 3 Foil clamp type 1 The foil clamp type 1 is appropriate both for clamping very small foil pieces and flat angular samples It is mounted in the standard specimen clamp Fig 31 40 Clamping of foil pieces Move the movable jaw 74 to the right as required by turning the set screw with an Allen key size 4 71 Place the foil 72 between the movable jaw 74 and the fixed jaw 73 To clamp the foil screw the movable jaw 74 against the fixed jaw 73 by using the Allen key Insert the foil clamp 75 in the standard Specimen clamp as shown Turn the knurled screw 66 clockwise to clamp the foil clamp in the standard speci men clamp Clamping of flat angular samples To clamp angular samples replace the long set screw 76 with the short set screw 77 provided with the foil clamp Unscrew the long set screw 76 to the left with an Allen key size 4 71 Screw the short set screw 77 in the hole Place the sample 67 between the movable jaw 74 and the fixed jaw 73 To clamp the sample screw the movable jaw 74 by turning the set screw 77 against the fixed jaw 73 Insert the foil clamp in the standard Specimen clamp as shown Turn the knurled screw 66 clockwise to clamp the foil cl
14. net 413 mm 300 mm 618 mm 305 mm with tray on the hood 100 mm measured from the base plate 168 mm measured from the table approx 3 kg 94 mm 164 mm 50 mm 81mm approx 0 450 kg Instruction manual V 1 5 11 2008 3 Instrument components and specifications Microtome Sectioning thickness setting Section thickness setting range Setting values Trimming section thickness setting range Setting values Object feed Vertical stroke Maximum sectioning area w o retraction Maximum sectioning area with retraction Specimen retraction in manual sectioning mode Electric coarse feed Repositioning of knife holder base north south Maximum specimen size W x H x D Specimen orientation horizontal vertical Leica RM2245 0 50 100 um from 0 50 um 5 0 um from 5 0 20 0 um from 20 0 60 0 um from 60 0 100 0 um in 0 5 um increments in 1 0 um increments in 5 0 um increments in 10 0 um Increments 1 600 um from 1 0 10 0 um from 10 0 20 0 um from 20 0 50 0 um from 50 0 100 0 um from 100 0 600 0 um in 1 0 um Increments in 2 0 um increments in 5 0 um increments in 10 0 um Increments in 50 0 um increments 28 mm 1 mm feed motion via step motor 70mm 65 mm without specimen orientation 60 mm 5 100 um in 5 um increments can be turned off 300 m s and 900 m s 24mm 50 x 60 x 40 mm 8 8 15 4 4 1 Standard delivery The
15. right of the baseplate The horizontal arm must be centered over the microscope e Slide the horizontal arm 3 flat side facing the lock screw g into the cross member d and tighten For more information on connecting and using the microscope magnifying lens or cold light source please see the appropriate manual 54 Instruction manual V 1 5 11 2008 6 Optional accessories 6 9 Magnifying lens The magnifying lens provides a 2x magnification and can be used with all Leica 2200 series rotary microtomes e Open the screw 3 on the horizontal arm of the microscope carrier in a counterclock wise direction e Insert the silver connection piece 1 as far as it will go Tighten the screw 3 e The adapter 2 allows a fiber optic light guide to be installed e Adjust the position of the magnifying lens to the specimen being processed The magnifying lens can be swiveled com pletely to the side if necessary Always protect the magnifier glass from exposure to direct sunlight Exposure to sunlight may cause a burning glass effect Danger of fire e Use the provided protective cap 4 to cover the magnifying lens Leica RM2245 55 6 Optional accessories 6 9 Cold light source The cold light source may be operated at the power supply voltage specified on the identification label on the underside of the instrument only e Connect the power cable 1 to the connect ing j
16. trimming section thickness setting In um Fig 14 Selecting the sectioning and trimming mode To switch between sectioning mode and trimming mode press the TRIM SECT button Whenever the button is pressed the display toggles be tween SECT and TRIM In the SECT display the sectioning thickness in the range from 0 50 to 100 0 um is shown and in the TRIM display the trimming section thick ness between 1 0 and 600 um Is displayed Setting the section thickness trimming section thickness g Coarse feed functions Leica RM2245 Adjust these settings using the keys on the control panel Section thickness setting range 0 50 100 um Setting values from 0 5 um 5 0 um in 0 5 um increments from 5 0 20 0 um in 1 0 um increments from 20 0 60 0 um in 5 0 um increments from 60 0 100 0 um in 10 0 um increments Trimming section thickness setting range 1 600 um Setting values from 1 0 10 0 pm in 1 0 um increments from 10 0 20 0 pm in 2 0 um increments from 20 0 50 0 pm in 5 0 um increments from 50 0 100 0 um in 10 0 um increments from 100 0 600 0 um in 50 0 um increments The electric coarse feed at two speeds Is used for a rapid movement of the specimen towards and away from the knife Press the buttons with the double arrow for a speed of 900 um s for 300 m s please use the buttons with a single arrow In sectioning mode the coarse drive can be used in STEP mode or with continuous feed Continuo
17. 0402 09307 Ee E EA E CK E A E I A I A A A E 14 0402 26922 Round specimen holder Silver ccccccccscesssssessessssesseeessessusecessrseesessesensaseesensansesesansaesess 14 0502 38001 Round specimen holder with adapter and 3 clamping rings silver sssssesssse 14 0502 38002 Clamping ring for round specimen holder d 6 MM sssssssssisssrsrririsisrsrnrerersrsrrrsrsrrrrrenns 14 0356 08322 Clamping ring for round specimen holder d 15 MM uu eee sees eee seen 14 0356 09200 Clamping ring for round specimen holder d 25 MM ccscesesseesseessessseessrsesereeseees 14 0356 08320 Leica RM2245 57 58 Optional accessories EM specimen holder fixture DIACK cccccscsesscsssessececsrssrsseseesessesascesersansessesensensasensensas 14 0502 29968 EM univ specimen holder ope cescetevecsscnastioasdacatuascesiaiseunecnusdShocedenuatenedteinaasnestueteesvenatetoocnans 14 0356 10868 EM flat specimen holder sasiscdscsnsncssttsetncintsoninathitiodastasntbaainntenaonsatiedbeanisasreidinandueibedindtuenianngs 14 0355 10405 Special wrench f EM holder ccesscscssessssecscsessesseceecessesseceeevseseesesseseesaseesanseseesensasenses 14 0356 10869 Universal microscope carrier complete cccccccecessscsecsssrssecsesessnsseseeeseesensersnsensanees 14 0502 40580 Magnifying lens assembly s sssssesessrsrersrsrurunununururununununununanananavuninannnnnrnnnnnrnrnrnnnrnrnnnnnrnrenens 14 0502 42790 Cold light sources Leica CLS 100X 100 120
18. EC Declaration of Conformity B P 4 N Ts g f h N _ er 7 gt o D AM aratian Q T l antarm I T V JeClafallon OL UONTOFMILY MICROSYSTEMS f i A 4 d NA i P A i x We herewith declare in exclusive responsibility that the Leica RM2245 Rotary Microtome was developed designed and manufactured to conform with the e Directive 2006 95 EC of the European Parliament and of the Council Low Voltage e Directive 2004 108 EC of the European Parliament and of the Council electromagnetic compatibility e Direktive 98 79 EC of the European Parliament and of the Council in vitro diagnostic medical devices The following harmonized standards were applied e EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements e EN 61326 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements e DIN EN 61010 2 101 2002 Safety requirement for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD e EN 14971 2007 Medical devices Application of risk management to medical devices e EN 591 2001 Instruction for use for in vitro diagnostic instruments for professional use In addition the following in house standards were applied e DIN EN ISO 9001 2000 Quality management systems Requirements
19. Specimen is clamped into place You can use the quick clamping system 64 to hold all available accessory specimen clamps for more information see Chapter 6 Optional accessories To do so proceed as follows e Move the object head 60 to the upper end position by turning the handwheel 12 and engage the handwheel lock e To release the clamping system turn the screw 61 of the quick clamping system 64 counterclockwise using an Allen key size 4 71 e Push the guide 63 of the universal cassette clamp 62 from the left into the quick clamp ing system 64 as far as it will go e To clamp the cassette clamp turn the screw 61 clockwise as far as it will go of the cassette clamp Since all stage clamps available as accessories are equipped with the same kind of guide on the back they are inserted in the same way described here using the example Leica RM2245 31 5 Operation 5 4 Adjusting the clearance angle A e The index marks 0 5 and 10 for adjust 4 Pa ment of the clearance angle 59 1 are located on the right side of the knife holder 57 ke e There is also an index mark 59 2 on the WADA right side of the knife holder basis 51 which serves as a reference point when adjusting D E the clearance angle e Loosen the screw 58 using an Allen key size 4 71 until the knife holder 57 can be moved e Move the knife holder until the index mark of the
20. The holder for round specimens is designed to accommodate cylindrical samples Inserts for specimens of 6 15 and 25 mm diameter are available AL Fig 36 To mount the required insert 89 1 3 turn the clamping ring 90 counterclockwise and remove it Place the required insert into the tension ring 90 and screw the tension ring onto the thread 91 by turning it clockwise Mount the sample 67 and fix by turning the clamping ring 90 clockwise To orient the inserted sample insert the pin 92 into the bore 93 and rotate it counter clockwise to release the clamp You can now rotate the specimen so that the side you want faces upwards To lock it in the position you have chosen tighten the pin 92 by turning it clockwise Instruction manual V 1 5 11 2008 6 Optional accessories 6 3 Knife holder base and knife holder Fig 37 6 3 1 Knife holder base without lateral movement feature Fig 38 Leica RM2245 The plastic handles of all clamping levers on the instrument and knife holders can be turned to the position that is most convenient for each user Pull the grip 94 out of the lever hold it in this position and rotate it to the desired position It will then lock automatically when released Repositioning the knife holder base The one piece knife holder base rigid 51 can be moved forwards and backwards on the microtome base plate
21. This vertical displacement allows bringing the knife holder into the optimal cutting position in relation to the specimen e To release rotate the clamping lever 50 on the right side of the microtome base plate counterclockwise e Reposition the knife holder together with the knife holder base forward or backward as appropriate e Secure the clamping mechanism by rotating the lever 50 clockwise 45 6 Optional accessories 6 3 2 Knife holder E E TC The knife holder E TC is designed for the Leica TC 65 tungsten carbide blades Knife holder E TC Fig 39 Prior to inserting the blade both knife holder and knife holder base must have been installed on the instrument Inserting the blades knife holder E and E TC Fold knife guard 9 downward To insert the blade flap the right clamping lever 10 forward and down Carefully insert the blade from the side Make sure that the blade is clamped parallel to the upper edge of the pressure plate To clamp the blade rotate clamping lever 10 back upwards i The knife holder E is designed for conventional disposable blades from all current manufacturers It with a blade ejector 9a is available in two models one for narrow band blades and one for broad band blades The knife holder has a lateral movement so that the entire width of the blade can be used It is also equipped Knife holder E
22. desired clearance angle coincides with the reference line on the knife holder base Enlarged detail Index marks for adjusting the clearance angle Example Enlarged detail showing a clearance angle setting of 5 The recommended clearance angle setting for knife holder E is approx 5 e Hold down the knife holder in this position and retighten the screw 58 for clamping Fig 23 32 Instruction manual V 1 5 11 2008 5 Operation 5 5 Clamping the specimen Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or specimen prior to changing the specimen block and during all work breaks A Always clamp the specimen block BEFORE clamping the knife e Rotate the handwheel until the specimen clamp is in the uppermost position Activate the handwheel lock by allowing the handwheel handle to lock in place and then activate the brake Insert a specimen block into the specimen clamp specimen clamps and specimen holders is provided in Chapter 6 i A detailed description for inserting the specimen into various Optional accessories 5 6 Clamping the knife disposable blade Be very careful when handling microtome knives or blades The cutting edge is extremely sharp and can cause severe injury Carefully insert knife or disposable blade into the knife holder and clamp Make sure that the blade is clampe
23. orauasstesecsananaconnacstecticenasetetdesandneesebesctunteventecaehadahmsandetveaneaesceens 14 0502 37993 Knife holder NZ RM22xx SIlVer sesesssssessssssnensnununennneninununanininannnrnnnrrsrnrurnnnrnrnrenernrererererenens 14 0502 37994 Knife holder E narrow band RM22xx silver with ejector uu ceessesseeseesseseteeseneeees 14 0502 40508 Knife holder E broad band RM22xx silver with ejector esseere 14 0502 40509 Knife holder E narrow band Run off tray RM22XX sassen 14 0502 38961 Knife holder E broad band Run off tray RM22XX seses 14 0502 39114 Knife holder E TOC RM22xx silver uu ccessssscsscsssssssecsessrssesseeseeseeseseesensaneessesensaesesensaneens 14 0502 37997 Standard specimen clamp with adapter 50X60 silver ou eects sseeeeteseeeteereen 14 0502 38005 Standard specimen clamp with adapter 40X40 silver uu cece seeeeseeereeteeereen 14 0502 37998 V insert for Standard Specimen clamp Silver ccccccessscessessssessssessessseesessneessesenseees 14 0502 38000 Universal cassette clamp with adapter silver oo cccceccessessscsscssscsecesseeneeresesensneess 14 0502 37999 Universal cassette Clamp ice cooled cccceessssssescssssscssessrsseseeseesesensetsesensateesensaeeens 14 0502 37793 Megaclamp assembly RM22xxX Silver ccsesesssssecscssssscecsessrsseseseeseesensesseseneatsesnsaesass 14 0502 38967 Foil clamp type 1 DIAC K asedscscvasinsestencvniasenonsonstaenssshainninssndtirentannsenavantinedapeniabeniiinetiecmaen 14
24. parts of the knife holder mixed up For cleaning and removal of paraffin do not use xylene or cleaning fluids con taining alcohol e g glass cleaner If several knife holders are cleaned at the same time the parts must not be Dry knife holder and reassemble After cleaning the moving parts apply a thin coat of drive part oil to them see also Chapter 8 2 3 When installing the pressure plate 83 ensure that the upper edge Is parallel to and level with the rear edge of the knife holder base 86 see also Fig 38 Page 45 63 8 Cleaning and maintenance Universal cassette clamp e Detach cassette clamp 13 for a thorough cleaning removing all paraffin residues e For cleaning do not use xylene Use xylene substitutes or paraffin removers such as Para Gard e The cassette clamp 13 can also be placed in an oven heated to a maximum of 65 C until the liquid wax escapes e Remove paraffin residues with a dry cloth e After such a cleaning procedure in an oven always be sure to lubricate the axle and spring of the clamping lever 60 see also chapter 8 2 3 Fig 63 8 2 Maintenance 8 2 1 Replacing fuses Switch the unit off and unplug it Use only the supplied replacement fuses Both fuses must have the same rating check the imprint e Insert a small screwdriver into the cutout 25 and carefully pry out the insert e Remove the voltage selec
25. specimen holder fixture only e ce cubes can be made using the included Paraflex mold a e Quick clamping system b see page 45 Instruction manual V 1 5 11 2008 6 Optional accessories 6 2 6 Super mega cassette clamp Fig 35 Assembly of the super mega cassette clamp The super mega cassette clamp should preferably be used with the rigid fixture for specimen clamps To do so proceed as follows i Remove the rubber ring 5 only after having attached the cassette clamp on the object head e Screw the rigid fixture for specimen clamps 4 onto the object head 3 Remove the screw 1 place the fixture for specimen clamps 4 onto the object head 3 from the front and tighten the screws 2 with an Allen key size 3 Next insert the screw 1 from the side and briefly tighten it with an Allen key size 4 e Insert the super mega cassette clamp from the side on the left into the dovetail guide of the rigid fixture for specimen clamps and tighten screw 1 knife holder base the orientation must be in position 0 and the cover for backlighting illumination must be detached Danger of collision if not observed A If the directional fixture for specimen clamps is used with the rigid NEVER use the super mega cassette clamp with backlighting illumination Leica RM2245 43 6 Optional accessories 6 2 7 Holder for round specimens i
26. that indicates the actual voltage setting of the instrument upon arrival Severe damage can be caused to the instrument if the voltage selector is set to an incorrect voltage e When changing the voltage selector setting ensure that the instrument is not connected to the mains power e Connect the instrumentto a grounded power socket only using one of the power cables provided e Do not operate in rooms with explosion hazard e Exposure to extreme temperature changes between storage and installation locations and high air humidity may cause condensation inside the instrument If this is the case wait at least two hours before switching on the instrument Failure to comply with this may cause damage to the instrument e The protective devices on both instrument and accessories must neither be removed nor modified A e Once removed from the crate the instrument may only be transported in an upright position Personal safety precautions e When working with microtomes personal safety precautions must always be taken It is man datory to wear work safety shoes safety gloves a mask and safety goggles 8 Instruction manual V 1 5 11 2008 2 Safety Warnings Working at the instrument extremely sharp and can cause severe injury e Always remove the knife blade before detaching the knife holder from the instrument Always put the knives back into the knife case when not in use e Never place a knif
27. to allow the instrument to adopt the ambient temperature before turning it on Failure to comply with this may cause damage to the instrument Connecting the power supply 20 e Before connecting the power cable make sure that the power switch 27 on the rear of the instrument is switched to O OFF e Various country specific power cables are provided with the instru ment Make sure that the power cable used has the correct plug for the power socket e Insert the connector of the power cable into the connection socket 26 and plug the power plug into the power socket When turning the instrument on with the mains switch do not press any of the buttons of the control panel Turn the instrument on with the mains switch at the rear This ts followed by a beep The instrument initializes The software version is displayed in the 4 digit display for approx two seconds Then the display reads 0000 After the microtome is switched on the display fields and LEDs of all activated functions are lit up on the instrument s control panel and display field The three digit LED display indicates the last value set for the section thickness or trimming section thickness depending on which setting was last activated This is indicated simultaneously on the control panel and microtome The LED of the active mode here the section thickness is illu minated in green If the yellow LED in the LOCK field 4 of the
28. 0 DZ TOS SVC VS EE E E A E E 61 8 Cleaning and maintenance qu cece ccecssscsesescesescscesescscessscesessssescssscescssecessstecestetscestesseestenes 62 8 1 Cleaning the instrument nceescicasinassensinninnasamastinncontddascauensa saucdienanvabrdeainisiniatanievanierndtasnsenstusesdiatiaisanadans 62 e2 Manen anna A E E 64 821 Replacing USES saccaissacharorsatcguanssengsdaychvncisensna vucaqnesnrsshagethaitsevansentiediaaetaaudiagraeeonashannstaniadioigueincerstearaneat 64 8 2 2 Maintenance instructions sicessanennanuanriesiesscasaancansonnaiiensaiionnscaen ticaanicdesunaninasshabyiteiinasigancesanetmestsnreed 65 8 2 9 Lubricating the instrument sccwsrasatssnesdvsscensesasnscinnsanacadtadnstaateeunivatndsnindvatiuastanscdaridetsuattednsaienshayinanonints 66 9 Warranty and service es cececccnzicevesss neencdactentivesveiqeateeiatnsandceniealoonteshateessdestubanlevinueietineamebsrnatanteenee 67 10 EC Declaration of Conformity 0 0 0 0 ccescecetsscesessscesesescessssscesssneeesssssesseceeesteceeeeteeneeeen 68 Peoples Republic of China cscs csssssssssssssssessessssssssssessessssscsessessssassscsecssssssassnssessssassaseessesess 69 Leica RM2245 5 1 Important notes 1 1 Symbols in the text and their meanings Dangers warnings and cautions appear in a gray box and are marked by a warn ing triangle A A Notes i e important user information appear in a gray box and are marked by i an information symbol il
29. 350 1 Instruction manual snisscsersnscesncentsatunnacssaatntanstapntonscaetseemniannaniugesbes 14 0501 80001 The accessories ordered are included in a separate box Carefully check the delivery against the packing list and the deliv ery note Should there be any discrepancy please contact the Leica selling unit handling your order Instruction manual V 1 5 11 2008 4 Startup 4 2 Installation site requirements e Stable vibration free laboratory bench with horizontal and even stage plate practically vibration free floor e No other instruments nearby which might cause vibrations Room temperature permanently between 10 C and 35 C Obstruction free access to the handwheel AN Never operate the instrument in rooms with explosion hazard Setting up the instrument N Do not transport the instrument by holding it on the handwheel shaft the object head or the cover e By holding the instrument 5 at the front by the baseplate and at the rear by the recessed grip Fig 8 lift it out of the molded cushion 6 of the packaging and place It on a stable laboratory table e The two sliding elements 9 located on the rear of the base plate make it easier to move the instrument on the table e To move the instrument hold it by the front of the base plate lift it up gently and slide it on its slides Leica RM2245 17 4 Startup 4 3 Assembling the hand
30. Leica RM2245 standard delivery includes 16 1 Leica RM2245 basic instrument without orientation 14 0501 38178 1 handwheel complete cccccessesseeesssssessesesesesersesesereeeen 14 0501 38181 1 external control PANE cece css eeeeseseeeseeenseesesereeees 14 0501 38179 1 section waste tray cnesenssicasnrsercastaaesscecavontideapenu naa inatoaasiesiasientaetasustevedeenean 14 0502 37931 1 set of power cables consisting of 1 power cable for Germany esssesssisrersrsrrisrrrrrrrrrrrrrrrrr 14 0411 36958 1 power cable for USA Canada Japan esssscccsncnrn 14 0411 36960 1 power cable for UK ST BU F 5A s ssssesereresesnsnererersrersrenness 14 0411 36959 1 tool set consisting Of ssesssssssssssesrrenennrerinirnrinirininnnnsrnrnrnrsrnrns 14 0502 37965 1 Allen key with handle size 5 uv ecsssesessssesssessnesesessneeees 14 0194 04760 1 Allen key with handle SIZ 4 sssscsssesirsirrr 14 0194 04782 1 Allen key SIZe aontar eae sentcetidsatesauteanecau aan dedi usnoneedleaneadatis 14 0222 04138 1 screwdriver 3X50 186 0ng cccesseetesseseeeeereeceeeeeen 14 0170 11568 1 bottle 50 ml of oil for drives type 405 eee 14 0336 06086 2 micro fuses 1 0 Af cssccscsussessirinisectnstsactarseienasrosiivasonurentintcdenned 14 6943 01001 1 brush W Magnet sssesssssesesirsrsrsrsrururunururnnununnrnnunarnnennnrsrererss 14 0183 40426 1 dust protective COVE ssssessssrsssssssrurrnururnrurunnrurununinananinininnsrnrernns 14 0212 30
31. V 50 60H2 siaticssiscassnectatinsnnessdunsstecnatesnsiinantennasnntacounsensestianeedi 14 0502 30214 Leica CLS 100X 230V 50 OU IAC oerrinne iein eA 14 0502 30215 Leica CLS 100X DAO FSO ONAL ocsi a a etainasvtath nagetnatitedevecess 14 0502 30216 Fiber optic light guide civstnestsnvaisstisatsivinetiv cura nn tioetaenivraatenintanteissaaeiteiviencestanened 14 0502 30028 Backighing assembly cerre N 14 0502 38719 External power supply complete ccecceesccssesssesscsrssrssesesessrsescessesensessesensensesensensanees 14 0500 31244 Handrest for waste tray wnncerecsectuteatcispan annus doendsonanteneediasuaneeadetinca dhetnnalsiesiaaatetieenninueneatanss 14 0502 38770 Cooling device assembly socsscascsotscnsaicastatsantvansneaentcteravastsisaersandntenscsasunnismsiaaensindaaadieiasniandtias 14 0502 38325 Section waste tray MIC Cire acest cacennne saa vecodnemsacievoectiacmetia iesanas tenet satasuntasbededenmndeeeceanarerere 14 0502 37931 Section Wa Sle tray Small sstsccassnasseeniaseusvesneatoyntanntanpaneraantiahcdardinahetumaiiaasnesapinneibbaestiaattaneia 14 0503 39060 Demo Box RM22XX Series a iscsnrterpiscacocnpsrettentenssteaninartecatecuie uaeiteennseysbetareananiernmctansnsunetoniats 14 0502 38930 WE E P AE E E EEA AEEA A A A EA P E A E EA A 14 0502 37932 Leica brush VU Filed OIG woes sadavamanascanconsnoranntuauvbttersubarecdaxenenaeaianemssanistamnsentaanneenanianseanates 14 0183 40426 Instruction manual V 1 5 11 2008 7 Customiz
32. ack on the rear of the device e Plug the power plug into the power socket Carefully read the instruction manual provided separately before use e Attach the fiber optic light guide 2 to the adapter of the magnifying lens and secure it in place in the bore 6 using the screw 5 e Pull off the protective cap 3 from the end of the fiber optic light guide and keep it in a safe place e Insert this end into the opening provided 4 until it engages with a slight click e Switch on the Cold light source using the power switch 7 e Remove the protective caps 8 and align the light guide with the specimen Fig 62 Leica rotary microtome with installed optional accessories magnifying lens and fiber optic light guide 56 Instruction manual V 1 5 11 2008 6 Optional accessories 6 11 Ordering information Fixture for specimen clamps rigid SIVEN ccccscssescsssscsessssrssessesesessteesesensesseseneeeeen 14 0502 38160 Fixture for specimen Clamps directional SIVEM cccccessssscssssssessserssesesersesesersneenes 14 0502 38949 Fixture for specimen clamps directional with 2 zero point indicators silver 14 0502 37717 Quick clamping System complete ccessessesesscecscsrssrseeseesesseseseesensaseesensansassesnseesens 14 0502 37718 Knife holder base rigid silver cesccscsessecscssesssseceseessessecesseserseesessrsensasersensansessesensansass 14 0502 37962 Knife holder N RM22xx silver
33. amp in the standard Specimen clamp Instruction manual V 1 5 11 2008 6 Optional accessories 6 2 4 Foil clamp type 2 The foil clamp type 2 is appropriate for large foil ribbons It is mounted in the standard specimen clamp e To open the jaws 78 and 79 lightly loosen the 3 screws 81 with an Allen key size 4 71 e Insert the foil ribbon 72 from behind to po sition it between the movable jaw 79 and the fixed jaw 78 e To clamp the foil first tighten the screw in the middle and then the other two screws 81 with the Allen key 71 e Place the foil clamp 82 in the standard specimen clamp so that the beveled surface 80 on the back of the foil clamp points to the right or left e Turn the knurled screw 66 clockwise to se curely clamp the foil clamp in the standard specimen clamp 72 78 79 80 Fig 32 Leica RM2245 41 6 Optional accessories 6 2 5 Universal cassette clamp 60 65 Order No 0502 37999 Fig 33 Universal cassette clamp ice cooled Order No 0502 37793 Fig 34 42 The universal cassette clamps UCC are designed for horizontal or vertical clamping of all kinds of commercial cassettes e Push the lever 60 forwards e Mount the cassette 65 horizontally or vertically as required e To clamp the cassette release the lever 60 Use the ice cooled UCC with the non directional
34. cen en 6 E A EA E E AA E A AEA EE AE E E E N 7 21 Saey VOLES nnan i i E a 1 22 WAS E E E A A 1 2 3 Integrated safety devices cccccessesssecscsssssssessesesessececsesseseececseserseesesseseeseusassesensescesensansensesenseneas 10 Instrument components and specifications c cece csssesescssessseseesssesseseesssessesssseaeseeaeeess 12 3 1 Overview instrument COMPONENTS cccecceesecseesssssscsseeessessneessesensescessnsensessesensensessesensensasensensas 12 3 2 Instrument specifications sriciscsactucguistnsessntbnaiediuastoinsbaiedtagioniatatngeahssaradnsiusbndagd iedetanatintaddbeimeaniataaeersin 13 Ir TIM Al Ch AC ed ages nese te on naactatavant eaapesaaaaatiaeantan ce teecaeagisnineiusesineswtsandasvanedaindsadsssteousiesateonnattdates 14 Ci ee 16 A Standard deveye Enoe NE cst mca ETNE A na 16 4 2 Installation site requirements c cccccceccsescssssssecsssrssessesesessesescesensensessesensessessnseesessesensanasensensansas 17 4 3 Assembling the handwheel sssesessssresenenenunenunununununnnnnrnrunununurunununununanununanananunnnnnnnnnnnnnrsnnrnrnnnnnnnnenns 18 BA TCC TIC Al CONG CO 11S seorsan i E ENTREE EEEE R 18 4 5 Switching on the instrument aecee c dens ceaatateenitanoctatesiesasetensesaalesiicamntlvarstele wetatenstobeaetunannecteneeuaeadvenniess 20 WEC E EE EA T E ET A E E E EE E E eecoeadee 21 5 1 Operating elements and their FUNCTIONS sssssesssssessnenenrsunenuninnnnnnnrnrnrrsrnrurnnurnrnnnnnnnrn
35. cesdicdeedayessis uadeantosiaiunnindietersacaneorseeaeies 36 6 1 Assembly for fixture for specimen clamps ssssssssssssssrsrsrsrsrsrunurunurununununarananannninnnrnrnrnrnnsrernrnrnrerenns 36 6 1 1 Rigid fixture for specimen CLAMPS ccccecsesssesscsserssececsessrsseseseseeseusessesensescesenseesessesensensessesenseneas 36 6 1 2 Directional fixture for specimen clamps ccccccsessscscssesssessesessessesescessessusessesenseesessrsensenassnsenses 36 Instruction manual V 1 5 11 2008 TABLE OF CONTENTS 6 1 3 Fine directional fixture for specimen CLAMPS ccccecsssssscscssssscsssessrssecessessnseesessrseesensetsnsensanens 37 6 14 Q ickclamping SYSTE cucsassasssasnsenscunscecesasiact enansnaineseidacnugtodsttadeutnchestlebiedindeeabeieeyatdtadactaseveieitvanntiess 38 6 2 Specimen clamps and holders sssssessssssnenenenunununununinununanininnnrunurunurununurnnununnnanananunnnnnannnnnrnrnrsrnrnnnnns 39 6 2 1 Standard specimen clamp esessssrsrerersrururerururununununanunanunnnannnnnnrununnrununununnnunanananananunnnannnnrnnnnnnnnnn nnne 39 PANVE SO a A A EN 39 BZ 3 FON CAND YDE loeien eoe EE AE 40 ZF FOE E A E A E 41 6 2 5 Univ rsal cassette claMp s issiesisinircisisrinsiiisrninceiieiioneusisions stuseiceasatanibaiadivtaoiatteiyeeenanraaienmaanads 42 6 2 6 Super mega cassette clamp ccccescsscscssseessessesecsecseseeseseeseesessesensasseseeseuseesessnseeseasessnsensassesensatens 43 6 2 7 Holder for round SpeciMenS siisscss
36. control panel is lit the mechanical handwheel lock or the handwheel brake Item 3 in Fig 3 is activated The instrument cannot be started as long as the LED is lit Instruction manual V 1 5 11 2008 5 Operation 5 1 Operating elements and their functions i The operating functions of the microtome are divided between a control panel and a display unit on the microtome A control panel on the instrument displays the current operating mode as well as various settings All operating functions are centrally located in the separate control panel All keys and displays are logically arranged in functional groups and easily identifiable 5 1 1 Instrument control panel RETRACT LED Lights up during specimen retraction Three digit display for displaying section thickness trim ming section thickness Four digit display of the section counter MENU MODE button Switches between section thickness sum and section counter MENU MODE CLEAR pressed simultaneously switches to the setting of the retraction value Leica RM2245 Fig 12 LED LOCK Lights up when handwheel locking mechanism is activated Green LED Lights up when trimming mode is activated Green LED Lights up when sectioning mode is activated Green LED Section thickness sum indicates the total for all sections Green LED Section counter displays the number of all se
37. ction 1 Thick thin sections The sections alternate between being thick and thin In extreme cases there are no sections whatsoever 2 Section compression The sections are very com pressed show folds or are squeezed together 3 Stripes in sections For knife holder E 4 Noises during sectioning The knife sings when sectioning hard specimens The sections show scratches or chatter marks 5 High blade consumption Leica RM2245 e Insufficient clamping of blade knife holder specimen or orientation e The blade is dull e The pressure plate is damaged or Incorrectly adjusted e Clearance angle of the knife blade too small e The blade is dull e The specimen is too warm e The sectioning speed Is too fast e There is an accumulation of paraffin on the rear pressure plate of the knife holder e The sectioning speed is too fast e The clearance angle Is too wide e Insufficient clamping of speci men clamp and or knife holder e Too great of a sectioning force was applied e Check the clamping positions and repeat the clamping process Laterally displace the knife holder or insert a new blade Insert a new pressure plate or use a new knife holder Methodically experiment with larger clearance angle settings until you have found the opti mum angle Use another area of the blade or a new blade Cool the specimen before sectioning Reduce the sectioning speed Remove
38. ctioning speed Predominantly high sectioning Slow to medium sectioning speed speed Specimen material Working with soft and hard Predominantly soft specimen specimens material Maintenance Every 12 months Every 24 months Note for inspection e When the instrument reaches approx 2 million performed strokes the code SEr always appears for approx 3 seconds in the three digit display when the TRIM SECT button is pressed switching between trimming and sectioning mode This is a reminder that the instrument must be inspected by an authorized Leica service technician regardless of how heavily the instrument has been used Leica RM2245 65 8 Cleaning and maintenance Fig 65 66 8 2 3 Lubricating the instrument Once a month lubricate the following parts with the included drive parts oil No 405 1 2 drops are enough Instrument and specimen holder e The clamping key 95 of the quick clamping system e The T piece 55 on the microtome base plate e Guide rails 96 for the knife holder base on the microtome base plate e The T piece on the knife holder base 56 Knife holder E e Clamping lever 11 for the lateral displace ment e T piece 88 and guide 97 of the knife holder for the lateral displacement e Clamping lever 10 for the blade Knife holders N and NZ e Sliding surfaces of the finger guard 8 e Knurled nuts 73 for measuring height adjustment
39. ctions CLEAR button Resets section counter and section thickness sum back to 0 21 5 Operation 5 1 2 Control panel Three digit display for section thickness trimming section thickness Buttons LED TRIM green Lights up when trim ming mode is activated for setting the section thickness trimming section thickness Button to enable and disable rocking mode Yellow LED Flashes during coarse feed backwards lights up when rear end position is reached Yellow LED Flashes during coarse feed forwards lights up when front end position is reached Coarse feed buttons Trimming mode Sectioning mode 22 Coarse feed Coarse feed backward fast Multiple step backward forward fast Multiple step forward LED SECT green Lights up when sectioning mode is activated TRIM SECT button For switching between sectioning mode and trimming mode Coarse feed forward slow Single step forward Coarse feed backward slow Single step backward Fig 13 Instruction manual V 1 5 11 2008 5 Operation 5 1 3 Display and control elements Three digit display m mm SECT This display is located both on the instrument TAM and on the control panel lf the SECT LED is lit up the display shows the Na SECT section thickness setting in um ME SECT if the TRIM LED is lit up the display shows the
40. d parallel to the upper edge of the pressure plate For more information see Chapter 6 2 2 Fig 36 A detailed description for inserting the blade or the knife into the individual knife holders is provided in Chapter 6 Optional acces sories Leica RM2245 33 5 Operation 5 7 Cutting into the specimen trimming ae Be FW 41 5 8 Sectioning Use the TRIM SECT key to select the trim mode Set the desired trim section thickness Release the handwheel lock and the brake In TRIM mode use the coarse feed buttons to move the sample against the knife blade Trim the sample by turning the handwheel or Press the ROCK button LED 41 in button is lit to select ROCK mode Trim the specimen by rocking the handwheel back and forth Terminate trimming when the desired sectioning surface and depth have been reached i Always turn the handwheel evenly in clockwise direction otherwise the brake will not work properly The rotation speed of the handwheel must be adapted to suit the hardness of the specimen For harder specimens use a slower speed Always use a different area of the cutting edge for trimming and sectioning To do so laterally displace the blade or knife in the knife holder 34 When using the knife holder E with lateral movement it is sufficient to move the knife holder sideways Use the TRIM SECT key to select the sectioning mode Adjust the appropriate section
41. der base must have been installed on the instrument Enlarged detail Knife inserted and height adjusted Knife holder NZ For holding conventional and hard metal knives up to 16 cm in length Knife pressure plate 56 for extreme stability and full utilization of the knife blade Knife holder N For holding conventional knives up to 16 cm in length Fig 44 Leica RM2245 49 6 Optional accessories Inserting the knife Knife height adjustment Rotate the knurled nuts 48 on the right and left of the knife holder forward in opposite directions lowering the knife support bar to the lowest possible position thus ensuring that the knife edge will not be damaged when inserting the knife Unscrew the clamping screws 49 as far out as possible rotate counterclockwise Hold the knife 47 at the knife back and carefully insert it in the holder from the side as shown with the cutting edge facing upward When adjusting the clearance angle the knife edge should be positioned as exactly as possible in the actual center of rotation of the knife holder The lay on edge 39 of the rear clamping chucks serves as a reference position for correct knife height adjustment The knife edge should be parallel with the locating edges Rotate the knurled nuts 48 uniformly and backwards until the knife blade is parallel to the lay on edge 39 see detailed illustration of the rear clamping chucks
42. e anywhere with the cutting edge facing upwards and never try to catch a falling knife e Always clamp the specimen block BEFORE clamping the knife or blade e Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or specimen clamp prior to changing the specimen block and during all work breaks e ALWAYS turn the handwheel clockwise otherwise the brake will not work properly e Always take appropriate safety precautions when sectioning brittle specimens Specimen may splinter e Ensure that liquids do not enter the interior of the instrument during work e Do not attempt to clamp approach or orient the specimen during the retraction phase If a block is oriented during retraction prior to the next section the block will advance by the retraction value PLUS the selected section thickness This involves the risk that the specimen collides with the knife blade A e Take care when handling microtome knives and disposable blades The cutting edge is Warnings Cleaning and maintenance instrument for service and repair e Before each cleaning switch off the instrument disconnect the power plug remove the knife holder completely and clean it separately Always remove the knife blade before detaching the knife holder from the instrument Lock the handwheel before each cleaning Do not use any solvents containing acetone or xylene for cleaning Ensure that no liquids enter the interi
43. e directional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps e Screw the 4 screws 13 into bore A with an Allen key size 2 5 and tighten them Fig 27 38 Instruction manual V 1 5 11 2008 6 Optional accessories 6 2 Specimen clamps and holders i All specimen clamps available as accessories can be integrated into either the directional or non directional specimen holder fixture 67 Fig 28 Fig 29 Leica RM2245 6 2 1 Standard specimen clamp The standard specimen clamps are available in two sizes 40 x 40 mm and 50 x 55 mm They are designed for direct clamping of rectangular blocks In addition they accommo date the foil clamps e Turn the knurled screw 66 counterclock wise to move the movable jaw 68 downward e Mount the sample 67 as required e Turn the knurled screw 66 clockwise to move the movable jaw upward against the fixed jaw to securely clamp the sample 6 2 2 Vee insert The vee insert 70 is mounted in the hole provided in the lower movable jaw of the stan dard specimen clamp This makes it possible to clamp round Specimens in the standard specimen clamp e Turn the knurled screw 66 counterclock wise to move the movable jaw 68 down ward e Insert the pin 70 1 of the vee insert 70 in the hole 69 of the lower jaw 68 e Mount the sample as required
44. e yellow LED 41 in the COARSE FEED button lights up Fig 16 e Feed stops once the front end position has been reached further sec tioning is no longer possible in that position You can continue to work on the specimen by pressing the corre sponding coarse feed button in the rear end position HOME and con tinuing with sectioning Caution To do so you must press TRIM SECT to switch to trimming mode i as otherwise you cannot use the coarse feed If the object head is already in the remaining feed range when the instrument is switched on an additional acoustic signal is heard after the software version is displayed e You can continue to work on the specimen by moving it back a short distance using the coarse feed buttons set trimming mode e The STEP function is disabled in the remaining feed range Leica RM2245 27 5 Operation Directional fixture for specimen clamps i In the quick clamping device of the directional specimen holder fixture all specimen clamps available as optional accessories can be used implemented The object orientation allows for simple position correction of the specimen surface when the specimen is clamped into place yl i Fig 17 Display of the zero position For better display of the zero position the orien tation has two red indicators 32 When both indicators are visible and both set screws are In zero
45. ed solutions 7 41 Instrument malfunctions 7 1 1 Error reports Leica RM2245 Certain instrument malfunctions appear on the 3 digit LED display or are indicated through illuminated LED s on the control panel Indication E1 lf this message follows the software version indication after switching on the instrument a loss of all parameters has occurred e Press any key All parameters are then set back to the default values e Now all settings must be checked to see if the previously entered values are still correct The values may have to be reentered You can continue with your normal working routine lf E 1 appears repeatedly when switching the instrument on the lithium battery may need replacement service life approx 7 years e Proceed as described above e Work normally after checking the settings e Have the battery replaced by Leica service as soon as possible LEDs 40 41 in the coarse feed buttons are lit at the same time This indicates a defect in the detection of the STOP and HOME positions of the object head The problem can only be resolved by customer service 59 7 Customized solutions 7 1 2 Malfunctions possible causes and troubleshooting i Following is a list of the most common problems which can arise while working with the instrument along with possible causes and troubleshooting procedures If the malfunction cannot be remedied with any of the options li
46. er and put it back in the knife case e Remove knife holder base and knife holder for cleaning e Remove the specimen from the specimen clamp e Remove section waste with a dry brush e Remove specimen clamp and clean separately Instrument and outside surfaces lf necessary the varnished outside surfaces of the control panels can be cleaned with a mild commercial household cleaner or soap water and then be wiped with a cloth To remove paraffin residue xylene substitutes paraffin oil or paraffin removers such as Para Gard Polysciences can be used The instrument must be completely dry before it can be used again Cleaning the knife A 62 Always wipe the knife from the back of the knife to the cutting edge NEVER wipe in the opposite direction risk of injury Clean using an alcohol based solution or acetone Instruction manual V 1 5 11 2008 8 Cleaning and maintenance Knife holder E Fig 62 Leica RM2245 Take the knife holder apart for cleaning To do so proceed as follows Fold the knife guard 9 and ejector 84 downward Rotate the clamping lever 11 of the lateral dis placement forwards and pull it out sideways Push the knife holder base plate 86 with the pressure plate 83 until it can be removed from the segment arch 87 Rotate the blade clamping lever 10 down ward and pull it out sideways Remove pressure plate 83 Clean all
47. f the voltage selector is set to an incorrect voltage Never change the setting of the voltage selector while the instrument is con nected to the power supply A Instruction manual V 1 5 11 2008 4 Startup Checking the voltage continued 99 The voltage selector is located above the main power switch on the left rear side of the instru ment Fig 10 The voltage setting is displayed in the viewing window 22 e Insert a small screwdriver into the cutout 25 and carefully pry out the insert e Remove the voltage selector housing 21 together with the fuses 23 Remove the voltage selector block 24 white and insert it again such that the correct local voltage Is displayed in the viewing window 22 e Reinsert the voltage selector housing with the block and fuses and push it in until it engages audible click Connecting the control panel The connecting cable 15 of the control panel is fixed to the microtome It may not be discon nected e Insert the plug 14 of the connecting cable 15 into the connecting jack 16 on the rear of the control panel e To fasten the plug tighten the two screws 17 Fig 11 Leica RM2245 19 4 Startup 4 5 Switching on the instrument A Exposure to extreme temperature changes and high air humidity may cause condensation to form inside the instrument After transporting please wait at least 2 hours
48. fied by Leica may repair the instrument and access the instrument s 2 2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention Primarily responsible for accident free operation is above all the owner of the instrument and in addition the designated personnel who operates services or cleans the instrument To ensure trouble free operation of the instrument make sure to comply with the following instruc tlons and warnings Leica RM2245 2 Safety Warnings Safety instructions warning labels attached to the instrument correct operating instructions as described in this manual must be followed when operating or replacing the instrument component bearing the label Failure to adhere to these instructions may result in an accident personal injury damage to the instrument or accessory equipment A e Safety instruction labels on the instrument marked with a warning triangle indicate that the Warnings Transport and installation e Never lift the instrument by the handwheels or the cassette clamp Always remove the section waste tray before transporting the instrument e Caution The voltage selector has been preset at the factory Before connecting the instrument to the mains power please check that this setting complies with the local power requirements of your laboratory The mains cable inlet is closed with an adhesive tape
49. ial prices for new clamp mounts in case of damages to the clamp mount after the warranty has expired In this way perfect function of the device can be ensured over the course of many years Setting of the clamping system of the clamp mount on the segment arch To ensure a proper sectioning result clamp mount B must be securely clamped onto segment arch A The clamping is carried out using an eccentric at the lever 11 The clamping force is adjusted with the setscrew 12 on the underside of the segment arch The setting of the clamping Is carried out so that the clamping lever can be rotated to the stop with constantly increasing resistance Leica RM2245 Adjust the clamping with a 2 5 mm Allen key at the grubscrew 12 so that the lever initially bars when the lever is activated Continue turning the grubscrew 12 a little bit approx turn to the left or right then check that the lever no longer bars but also does not jam heavily The language CD features a short video film for this purpose 4 6 Optional accessories Knife holder E with water bath for narrow band and broad band blades 11 Fig 42 Knife holder E with water bath is available for both narrow band and broad band blades The knife guard on knife holder E consists of a red foldaway handle 9 To cover the cutting edge fold the knife guard handle 9 upwards as illustrated in Figure The clamping le
50. ing thickness or verify the selected value Select conventional sectioning or ROCK mode To section the specimen turn the handwheel clockwise smoothly or rock it back and forth if in ROCK mode Pick up the sections and mount them on microscope slides Instruction manual V 1 5 11 2008 5 Operation 5 9 Changing the specimen or interrupting sectioning A Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or object head as well as prior to changing the specimen block and during all work breaks Raise the specimen to the upper end position and activate the me chanical handwheel lock Cover the sectioning edge with the knife guard Remove the specimen from the specimen clamp and mount a new sample to continue Before cutting into a new specimen move the object head back to the rear end position 5 10 Finishing daily routine Leica RM2245 Raise the specimen to the upper end position by turning the handwheel and engage the handwheel lock from the instrument Always put the knives back into the knife case when not in use Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife A Always remove the knife blade before detaching the knife holder Remove the blade from the knife holder and insert it in the receptacle at the bottom of the dispenser or remove the knife from the knife holder and pu
51. ion thickness sum When the n LED is lit the display shows the number of all previously completed sections Fig 15 e To change the display mode push MENU MODE until the LED of the desired mode is illuminated e Press CLEAR to reset section thickness sum or section number e This will only reset the currently displayed value Caution When the instrument is switched off using the main power switch both values section thickness sum and section number are erased from memory Specimen retraction To prevent damage to the blade and specimen the specimen is moved away from the blade during the return motion to the upper home position The retraction value can be selected in 5 um Increments between 5 and 100 pm Specimen retraction is set to 10 um at the factory Specimen retraction can be disabled if required The selected setting is maintained when the instrument is turned off Leica RM2245 25 5 Operation Configuring the retraction settings a e To call up the retraction settings press the MENU MODE and CLEAR Mos Cee buttons simultaneously e The current set value is displayed as a three digit number in the four digit display e g 025 25 um Select the desired retraction value The retraction value can be adjusted in increments of 5 um to a maxi mum of 100 um using the buttons on the control panel e To exit the retraction settings press MENU MODE A retraction mo
52. nnnunnrnnererrnenens 21 5 1 1 Instrument control panel otsorsciachenieeputanincaseadnnsthedeta deviisansdadidinaennshstatedbrninaesdagitisdiahuisatinetotiausiwacnattrenita 21 SLA CONU OLDAN iarra aE E E E E EE 22 5 1 3 Display and control elements sssssesssesesrsrsrerersrsrunununununnnununununununananananuninannnininnnrsrnrnrnrnrnnnrnrernrrneena 23 92 inserting the knie holde Eanes sioaseacssanpcs pnimvneroctanesacaestaniedamcnaasabioasaaneain eavinedesaniamamaastareanesmecueeni 30 5 3 Inserting the universal cassette clamp cccesssseccsssssssecsserseseeseseesseseseessesensessesensensessasensenees 31 5 4 Adjusting the clearance angle wiscsinsasisessnniasancinssastuatalanreiacadiinenstnithiensiearaeatesiuasnnsaisnnaiennediecaduatnstituentoats 32 5 5 Clamping the Specimen sicsccsccsesssaceanvaieneceesaareinesaanseis oneetiaunsdtentnanedamagevtcntanaideaeiaannatoinenvaanbiandinure Gadi 33 5 6 Clamping the knife disposable blade uu cccsssesscscssssscsessssrsececsesessesescessesensessesenseesensesensenses 33 5 7 Cutting into the specimen trIMMING ssssssssssrsrssrsrernsnsnsrnununsunnnunnnnununnnnnununnnnunnnnnnnnnnnnnnnnnnnnnsnnnnnns 34 oo ECUNI orrae EEE EEN EE RE A E 34 5 9 Changing the specimen or interrupting SECTIONING ccccessesceseessesecesseserseecesrsstseesessnseesanees 35 GF MALS UNINC dally TOUTE sisrsiriarissarieann iein Ea oian cionari 35 Optional accessories ss ciccscsczesecseenesdearetenocvsctaizictoniatacsextactutnassa
53. nssinscssnensitetuetesansneanestosaictudsatatdnetnehstbveiesinnatieeiainateindistassboiusiuanatisd 44 6 3 Knife holder base and knife holder cecccscessssssssscrssessesessesseceeceseesensessessnseeseusesensensasensensatens 45 6 3 1 Knife holder base without lateral movement feature cccecccccecsssscscecsrssrseseseesseseesessneenees 45 6 3 2 Knife holder E E TC n rea avirtcazsatcarats ease nas ancatraciteune vas uadeaeg a a a i AE Nae 46 633 K fe Nolder N N eserse oeadna iaar A eE Ea TEAR Naena SEa 49 DA SC OI WSE a a E E T A T E 51 OCF U oe A E a E 51 OD TN e a A E enasvanndas 52 O FOLEY DAC eenn E EA E AA 52 6 8 Universal microscope carrier sssssesessrsrerssurururununananananunnnannnnnnnununununnnununanunananananavunanannnnnrnnnnnnnnnnnn ne 53 eS CIV IG ICNS acacesia A a a A a AR g 55 ons Cod On SOUNGE cosi A E EAE E 56 610 FIDET OPUC WOME GUIDE sciucra socssasntascrsalsiupontinsiadnatnescoracduniamseibneientuabiacabinbhidintindidonieakandteieipentstagaatanendds 56 Beli OEE FUNC UPON Mea U ON sese E E A E E 57 7 Customized solutions oo ccc cess cscscesessssscssessssscsceesssessecesssetsenessssecsensessevenneeeeneteeneess 59 Ll MS UCU OT IT aU CONN S ct cesteaninees consis ccaiesaatuutulesaniaeiccuanccvastanien ussossaaiessecvaasivisdenaaendeumaceietenigaatounaes 59 LLENO We OU oreren EE E E E aucautuitends 59 7 1 2 Malfunctions possible causes and troubleshooting eesesssssssesesrrerrrrrrrrerriririisisrsrsrersrsrsrrrerenn 6
54. ocking specimen holder mechanism fixture with quick clamping system Smooth turning handwheel Lever of handwheel brake Knife guard on Separate control panel the knife holder pit Section waste tray Clamping lever for lateral displacement Knife holder E with lateral movement Knife holder base Fig 6 12 Instruction manual V 1 5 11 2008 3 Instrument components and specifications Rear side of instrument Magnet for holding the wrench size 4 Voltage selector switch with fuses Main switch Power connection 3 2 Instrument specifications The Leica RM2245 is a semi motorized rotary microtome with a light action handwheel The RM2245 supports two manual sectioning modes rocking mode and conventional sectioning with full turns of the handwheel All important controls are grouped in a user friendly manner on a separate control panel with adjustable inclination Important information on the operating status of the instrument such as retraction locking trimming thickness section thickness and counters for the total number of sections and total thickness sectioned is displayed directly on the instrument The instrument features two independent hand wheel locking systems for enhanced user safety lt also features an adjustable retraction system Leica RM2245 Vents Connecting cable for control panel Fig 7 The retraction value can be selected as required
55. opriate direction Longer activation of the double arrow coarse feed button effects a repeated feed motion for as long as the button is pressed In the trimming mode the coarse feed buttons operate a continuous movement as long as the button is held down The double arrow button for rapid coarse feed backward movements has a lock in function To start the rapid backwards movement away from the blade press the button After the button is pressed the object head is moved to the rear end position To stop the movement press any of the four coarse feed buttons The yellow LED 40 in the button flashes while the object head is in motion and remains lit continuously when the rear end position is reached Press the button to start the slow backwards movement The travel continues as long as the button is held depressed Press the appropriate button to start a rapid or slow forward movement The travel continues as long as the button Is held depressed During the forwards movement the yellow LED 41 in the button flashes When the front end position is reached an acoustic signal is heard and the LED stops flashing and remains lit Instruction manual V 1 5 11 2008 5 Operation Four digit display on the instrument The four digit display is adjustable When the pm LED is lit the display shows the sum of the section thicknesses in um for all sec tions completed since the instrument was switched on Sect
56. or of the instrument when cleaning Do not turn the instrument on before it is completely dry When using detergents please comply with the safety precautions of the manufacturer Turn the instrument off with the mains switch and pull the mains plug before replacing the fuses Only use fuses of the same specification For fuse specifications refer to chapter 3 3 Technical data N e Only authorized and qualified service personnel may access the internal components of the Leica RM2245 9 2 Safety 2 3 Integrated safety devices Fig 2 RETRACT 10 Locking the handwheel There are two ways of locking the handwheel 12 Using the lever 3 on the right side of the micro tome base plate the handwheel can be braked in almost any position e To brake rotate the lever in a counterclock wise direction to position A Caution The braking lever 3 must be exactly in position so that the handwheel brake is applied correctly If the lever is moved beyond this point it is possible that the handwheel is no longer braked e To unlock the handwheel brake turn the lever 3 back to its original position Position O e To lock the handwheel press the lever 5 outwards and continue to turn the hand wheel slowly until it locks exactly in the 12 o clock position In both cases the yellow LED 4 in the LOCK will be lit and the instrument cannot be started
57. paraffin from this area on a regular basis Select a slower sectioning speed Methodically reduce the clear ance angle setting until you have found the optimum angle Check all screw and clamp connections on the specimen holder system and the knife holder If necessary tighten the levers and screws Adjust the sectioning speed and or section thickness when sectioning Select a thinner section 61 8 Cleaning and maintenance 8 1 Cleaning the instrument A Always remove the knife blade before detaching the knife holder from the instrument Always put the knives back into the knife case when not in use Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife When using cleaners comply with the safety instructions of the manufacturer and the labor safety regulations of your laboratory When cleaning the outer surfaces do not use xylene scouring powders or solvents containing acetone or xylene Xylene or acetone will damage the finished surfaces Ensure that liquids do not enter the interior of the instrument during cleaning Before each cleaning carry out the following preparatory steps e Raise the specimen clamp to the upper end position and activate the handwheel lock e Switch the unit off and unplug it e Remove the blade from the knife holder and insert it in the receptacle at the bottom of the dispenser or remove the knife from the knife hold
58. pletely unscrew the thrust piece 5 with a flat tip screwdriver and pull it out with spring 5a and pin 5b e Completely unscrew the setscrews 3 and 4 e Attach the directional fixture for specimen clamps as shown e Insert the screws 7 8 in the bore 2 screws 8 are accessible through the bore 9a and evenly screw them in using an Allen key size 3 e Insert the spring 5a and pin 5b with the flatter side into the thrust piece 5 Complete ly screw in the thrust piece with a flat tip screwdriver e Completely screw in the setscrews 3 4 Instruction manual V 1 5 11 2008 6 Optional accessories Leica RM2245 6 1 3 Fine directional fixture for specimen clamps e Before the fine directional fixture for specimen clamps can be mounted loosen 4 screws 10 Allen key size 3 and carefully remove the fixture for specimen clamps from the baseplate 9 e Using the 4 supplied screws 11 and the Allen key size 3 fasten the baseplate to the object head 12 e Now screw the fine directional fixture for specimen clamps with the 4 screws 10 and the Allen key size 3 onto the object head If the fine directional fixture for specimen clamps is not used retain the baseplate and 4 screws 11 together with the fine directional fixture for specimen clamps 37 6 Optional accessories 6 1 4 Quick clamping system lt is used as specimen holder for use with the fin
59. position at the same time notch point white marking on the speci men Is in zero position When the large standard specimen clamp 50 x 55 mm is used the speci men orientation of 8 in north south direction is no longer possible The usable angle is only about 4 in this case i 28 The directional specimen holder fixture may be exchanged for a _ non directional fixture optional accessory Orienting the specimen A Specimen blocks must not be oriented during the retraction phase If a block is oriented during retraction the block will advance by the retraction value PLUS the selected section thick ness before the next section This may cause damage to both specimen and knife e Raise the object head to the upper end posi tion and activate the handwheel lock e To release the clamp turn the eccentric lever 29 forwards e Turn setscrew 30 to orient the specimen in north south direction Turn setscrew 31 to orient the specimen in east west direction Each complete turn of the screw inclines the specimen by 2 A total of 4 complete turns 8 are possible in every direction The accu racy Is approximately 0 5 For ease of estimation there is a white marking on the handle and a notch point that is noticeable during turning e To lock the current orientation turn the ec centric lever 29 backwards Instruction manual V 1 5 11 2008 5 Operation
60. rnet http Awww histo solutions com Leica RM2245 These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice Only in this way is It possible to continuously improve the tech nology and manufacturing techniques used in our products This document is protected under copyright laws Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photocopy microfiche web cam or other methods includ ing any electronic systems and media requires express prior permission tn writing by Leica Biosystems Nussloch GmbH For the instrument serial number and year of manufacture please refer to the identification label attached to the instrument Leica Biosystems Nussloch GmbH TABLE OF CONTENTS SCG Ci E EEE E E E E E EE 6 1 1 Symbols in the text and their meanings ou cccccecsssscsscessessececsessesseceeessesseseseeseesaneessesenseesessneateess 6 1 2 Qualification of personnel sc tetcara sperancerscn ea estat anenesdascatslenachaninnnt pea unren citer nreeiesaeterlaeednetnnercadedeasartounentai 6 1 3 Intended use of instrument sesocesscrenecucasieestesvsirrstvavacausaesosaestverevaacuansdequtensareteeias aemeateeentispeersisetneass 6 E E a 0 earns E E E E N A E A E eawn
61. sted in the table or the problem occurs repeatedly inform the Leica Service Support immediately Problem Possible cause Corrective action e No display no reaction to buttons pressed after the Instrument is switched on e Feed motion no longer occurs e The specimen in the residual path area cannot be returned using the coarse feed buttons 60 1 The power cable is not connect ed correctly Power fuses are defective The cable of the control panel is not connected correctly The voltage selection switch is set incorrectly The end of the residual path has been reached The specimen was already within the residual path when the instrument was switched on STEP mode is still active LED in SECT button lights up 1 Check the connection of the power cable Replace power fuses see 8 3 Check the connection of the cable to the control panel Check the voltage setting and correct it if necessary see Chapter 4 4 Select the trim mode by pressing TRIM SECT Then return the specimen using the coarse feed button Then retract the specimen using the coarse feed button Follow the procedure in Item 1 Press the TRIM SECT button to switch to trimming mode Return the specimen using the coarse feed button Instruction manual V 1 5 11 2008 7 Customized solutions 7 2 Possible faults Problem Possible cause Corrective a
62. t it back in the knife case Remove the specimen from the specimen clamp Push all section debris into the section waste tray and empty the tray Switch the instrument off at the main power switch Clean the instrument see Chapter 8 1 35 6 Optional accessories 6 1 Assembly for fixture for specimen clamps i Depending upon the purchase order the basic instrument is delivered with the directional or rigid fixture for specimen clamps which must be assembled first All specimen clamps available as accessories can be used in both fixtures for specimen clamps Before assembling the fixture for specimen clamps activate the mechanical handwheel lock 2 A 4 di mri 7 ii ii 9a 9a Fig 25 e Finally place the dovetail holder 2 and fasten by screwing in the 4 screws 1 using an Allen key size 3 36 6 1 1 Rigid fixture for specimen clamps e Screw the rigid fixture for specimen clamps 4 onto the object head 3 Remove the screw 1 attach the fixture for specimen clamps 4 onto the object head 3 from the front and tighten the screws 2 with an Allen key size 3 Next insert the screw 1 from the side and briefly tighten it with an Allen key size 4 i 6 1 2 Directional fixture for specimen clamps Remove the rubber ring only after attaching the object head e Loosen the eccentric bolt 6 by turning it counterclockwise e Com
63. tor housing 21 together with the fuses 23 e Remove the fuses While doing so leave the voltage selector switch block 24 in the housing e Replace defective fuses and reinstall the voltage selector into the instrument e Check that the correct voltage value Is displayed in the viewing window 22 64 Instruction manual V 1 5 11 2008 8 Cleaning and maintenance 8 2 2 Maintenance instructions Only authorized and qualified service personnel may access the internal components of the instrument for service and repair The instrument is basically maintenance free To ensure trouble free operation of the instrument over a long period of time the following Is recommended by Leica 1 Thoroughly clean the instrument on a daily 3 Have the instrument inspected on a regular basis basis by a qualified service technician 2 Enter into a service contract at the latest at authorized by Leica the end of the warranty period For more in The intervals depend on how heavily the formation please contact your local Leica instrument is used technical service center We recommend the following inspection intervals depending on the general workload of the instrument as defined in the three categories listed in the table Category Category Il Number of sections per day gt 8 000 sections per day lt 8 000 sections per day Workload hours per day gt 5 hours per day lt 5 hours per day Se
64. ual V 1 5 11 2008 2 Safety A other Leica products Be sure to comply with the safety instructions and warnings provided in this chapter Be sure to read these instructions even if you are already familiar with the operation and use of 2 1 Safety notes This instruction manual contains important instructions and information regarding the operational safety and maintenance of the instrument The instruction manual is an important part of the product which must be read carefully prior to startup and use and must always be kept near the instrument If additional requirements on accident prevention and environmental protection exist in the country of operation this in struction manual must be supplemented by appropriate instructions to ensure compliance with such requirements This instrument has been built and tested in accordance with the safety regulations for electrical measuring control regulating and laboratory devices In order to maintain this condition and ensure safe operation the operator must observe all the instructions and warnings contained in this instruction manual For current information about applicable stan dards please refer to the CE declaration of conformity on our Internet site www histo solutions com A internal components The protective devices on both instrument and accessories may neither be removed nor modified Only service personnel quali
65. us feed Is the default setting 23 5 Operation Sectioning mode Button functions in STEP mode Multiple Single step step backward backward Multiple Single step step forward forward Trimming mode Backwards coarse feed 40 In sectioning mode the user can select between STEP function step by step specimen feed and continuous specimen feed When continuous feed is selected the coarse feed buttons have the same func tlons as in trimming mode The STEP function is useful for careful step by step approximation of the specimen towards the blade How to activate the STEP function Switch the instrument on while holding the button on the control panel Likewise to deactivate switch the instrument on while pressing the button While the instrument is initializing hold the button until the software version number is no longer displayed ensure that version number 2 1 was displayed This feature is only available in sectioning mode in version 2 1 or higher If you have an earlier software version please contact Leica Service Press TRIM SECT button and select sectioning mode LED SECT lit When pressing a slow speed coarse feed button with an arrow in STEP mode the specimen moves towards or away from the specimen by the value indicated on the display single step By short activation of a double arrow coarse feed button a single step is also effected in the appr
66. vement takes place by the newly selected value after each section While the specimen is in retraction the yellow LED on the RETRACT display 4 lights up CTD TM ETRACI he J i Nall Switching off the specimen retraction e To call up the retraction settings ppess MENU MODE and CLEAR simultaneously Wenu s Mode T Cieni e To switch off retraction press the button on the control panel until the display indicates OFF e To exit the retraction settings press MENU MODE When retraction is switched off the specimen Is not retracted The yellow LED 4 of the RETRACT indicator does not light up eer TA RETRACT FIL dii Er 26 Instruction manual V 1 5 11 2008 Indication of remaining horizontal feed 5 Operation The visible and audible remaining feed indica Object head tion feature informs the user during trimming and sectioning when a remaining feed of ap proximately 1 mm is available before the front limit is reached The yellow LED 41 in the COARSE FEED button lights up from the beginning of the remaining feed In addition an acoustic signal is heard for Remaining horizon approx 2 seconds tal feed 1 mm o From this point on a remaining feed of approx 1 mm is available H Total horizontal feed In the remaining feed area no more object feeding to the knife is possible using the coarse Fig 16 feed buttons e You can continue with your working routine Th
67. vers on the knife holder are not interchangeable The two clamping levers 10 and 11 must remain in the position shown at all times as otherwise isolated malfunctions of the knife holder can occur Clamping lever for the blade 10 at the right clamping lever for the lateral displacement Use 48 Floating thin paraffin sections for example for subsequent immunostaining procedures on the surface of the water Flat paraffin sections can be removed from the surface of the water using glass slides The vessel is filled with water up to the blade After trimming remove the section waste from the tray and create the sections to be prepared The sections floating on the surface of the wa ter can be removed using the glass slide Fig 43 Instruction manual V 1 5 11 2008 6 Optional accessories 6 3 3 Knife holder N NZ c and d up to 16 cm long The integrated height adjustment feature allows you to also use knives i The knife holders N and NZ are appropriate for standard steel and tungsten carbide knives profile that have been resharpened numerous times Mounting the knife support bar e Push knife guard 8 to the center e Setthe knife support bar 46 onto the height adjustment screws not visible in the position shown The flat ends of the height adjustment screws must be located in the slots at each end of the knife support bar Prior to inserting the knife both knife holder and knife hol
68. wheel The handwheel has to be assembled before attempting to use the instrument The necessary parts and tools can be found in the toolkit 44 Electrical connections The feather key 4 is loosely placed in the handwheel shaft 1 and fixed in place with a tle rap during transport e Remove the cable tie Caution Make sure not to lose the feather key e Place the handwheel 2 on the handwheel shaft 1 as shown e Tighten the screw 3 located in the center hole of the handwheel with an Allen key size 4 5 e Remove the cover foil from the self adhesive cover disk 6 and fix the cover disk on the handwheel A cable The instrument MUST be connected to a grounded power socket Use only the power cable provided that matches the power supply outlets of the country of use Do not use an extension Checking the voltage The Leica RM2245 can be connected to various electrical power grids depending on voltage and frequency and for this reason is always delivered with a set of various power cables New instruments are factory set to 230 volts This is documented by a yellow label 230 VOLT on the rear of the instrument which covers the power switch and power socket 18 Before connecting the instrument to the power supply be absolutely certain to check that the voltage selector is set to the voltage in use in your area Severe damage can be caused to the in strument i

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