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1. 2 26 CENTRIFUGECDESCRIPTIONC Continued A green light emitting diode LED for SPEED and a yellow LED for POWER adjacent to the ON OFF button provide visual indicators of power circuit status Condition of each LED On Off or Flashing based on the status of power lid latching rotor speed etc is described in Sections 4 5 and 9 5 2 30POWEROPACK The Power Pack for the QBC Centrifuge is a table top 100 watt switched mode power supply with a universal input range 90 265 VAC and a 47 VDC output The unit is equipped with an on off switch and a user serviceable primary fuse 2 40SPECIFICATIONS 2 4 10 QBC c Centrifuge L Rotor Capacity 1 to 20 QBC tubes L Rotor Speed 12 000 rpm 80 rpm O Nominal Relative Centrifugal Force RCF 14 387 x g O Timer Electronic 300 s spin 15 20 s deceleration O Electro Mechanical Safety Interlock Lid must be closed and latched before motor can be energized Lid remains locked until rotor stops O Operating Temperatures For QBC Hematology 20 to 32 C 68 to 90 F For Parasite Detection 16 to 37 C 60 to 99 F O Functional Range Centrifuge Operation 2 to 40 C 36 to 104 F O Operating Relative Humidity 0 to 80 for temperatures between 2 and 31 C derating linearly to 50 between 31 and 40 C O Non Operating Storage Temperature Limits 26 to 66 C 15 to 150 F O Maximum Altitude 2000 m 6562 ft
2. O Weight with rotor and cover installed 2 8 kg 6 2 lbs O Dimensions with lid closed 23 5 cm W x 29 8 cm D x 12 7 m H 925 in W x 11 75 in D x 5 in H O Transient Overvoltage Category I L1 Indoor or under cover use only O Pollution Degree I O Electrical 47 VDC 3 VDC 3 ADC Maximum Steady State 2 4 20QBC yPowertPack The power supply accompanying or sold with the QBC Centrifuge complies with IEC 1010 2 020 and bears the GS mark O Input 100 240 10 VAC 50 60 Hz L Output 47 3 VDC 3 amps steady state 5 amps surge O DC Output Cord 0 46 m 1 5 ft O AC Line Cord unassembled e Standard Cord 2 3 m 7 5 ft e Continental European Cord 2 5 m 8 2 ft O Fuse 5 0 Amp 250V F type For temperature specifications consult the package insert of the disposables in use in your laboratory 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 6 F Sectiono3 INSTALLATIONCANDCSET UP 3 10PACKAGINGCANDoOPARTS The QBC Centrifuge and Power Pack are shipped in a single container together with the following parts test accessories and instructions Figure 3 1 Rotor Cover Assembly Spare Fuse for Power Pack Head Nut Wrench Two Power Pack Line Cords see below Rotor Removal Wire Operating Instructions this Manual Note For use of the centrifuge in Continental Europe an alternate line cord is provided with the Power Pack Select t
3. The QBC Malaria test is a qualitative method for diagnosing positive or negative patients While numerous reports have demonstrated that the QBC test is significantly more sensitive for detecting malaria than stained blood films QBC may not detect very low levels of parasitemia Experienced microscopists can discriminate between various species of malarial parasites using the QBC test Examination of a thin blood smear may be required to differentiate species in some cases See the package insert in the Malaria Kit for a list of literature references IMPORTANT For room temperature specifications consult the package insert of the disposables in use in your laboratory 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 19 F Sectiono8 CALIBRATIONOCHECKS According to Federal Regulations centrifuges such as the QBC Centrifuge require verification or calibration as follows 1 before initial use 2 after repair or adjustments and 3 annually after use The centrifuge timer however should be checked for accuracy at least every 3 months The QBC Centrifuge has been fully calibratedcatathecfactory and is ready to deliver consistent accurate test results upon first use For subsequent calibration checks follow the procedures in 8 1 and 8 2 below 8 10 CHECKINGCROTORCSPEED The rotor of the QBC Centrifuge is designed to spin at 12 000 80 rpm Atcleastteverya umonthsi
4. 2 1 3 1 QBC Centrifugal Hematology Tests 0 000 cece ett tte eee 2 1 3 2 OBC Malaria Detection Tests ii oues ecg aay ees nabs Pew Palade ewe e d Cas dw OR E ea 3 1 4 WARNINGS AND PRECAUTIONS 0 00000 cc cece eee RR Res 3 15 SYMBOL DEFINITIONS va retno Se eee uir yaa lates engin athens ware atte eee eee at ae Sette aoe ee eae 3 SectionoO2ZOSYSTEMODESCRIPTION 1 0 0 0 eee eee eee e 4 23 COMPONENTS iris epee et eres eects at te are at auch bates Ml Dd EM Id ig eer as 4 2 2 CENTRIFUGE DESCRIPTION te REIS REEOMLRIRE eee REEL Hee ee eee REX 4 2 3 POWER PACK 0 0 ccc ccc ee ee eee rne 5 2A SPECIFICATIONS iuit eese turp unser tees seal c E BAS LEM LAT EET aes ae GA Sa eas 5 2 4 1 QBC Centrifuge Model 425740 0 0 cee tenn eee n tenes 5 24 2 QBC Power Pack NO 42474001 acc cdc ct ced idee eed bu Ow been Gb edu dees cher Deve eda x 5 Sectiono3SoOINSTALLATIONGANDOSET UP 0 sehr nn 6 3 4 PACKAGING AND PARTS ccd ceed eos Bak ete Bek ets A Ea Pad Een Bale Ba nee ae 6 3 2 INSTALLATION AND SERVICE 0 0 00000 c ccc RR RR RR rr 6 3 3 CONNECTINGHPOWER UNIT 3 2 ro stiter ace eate carte ieee oe tele eee ste a ers ees IU TTE 7 3 4 PLACEMENT AND OPERATING ENVIRONMENT 00000 RR RR RR RR eee eee eee eee 7 3 5 ROTOR ASSEMBLY 0 0 ccc cc RR Rhea 8 Sectiono40CENTRIFUGECOPERATION 00 00 ee Rhe 9 Ae AGENERAL PROCEDURES mss oreet as oe ote Stig eee ee se et Ll ao Oo ase ai oe eee E 9 4 2 BETWEENG
5. LAYER AROUND FLOAT PLATELET LAYER DARK RED CELLS PLASMA White Label PRIMARY MALARIA FLOAT S SECONDARY MALARIA DETECTION ZONE DETECTION ZONE 55 65 uL Blue Fill Lines Figure 1 3 Differentiated Cell Layers in Spun QBC Malaria Tube Examination of the centrifuged blood under a fluorescence microscope readily permits the detection of malaria in the infected cells and plasma Direct malaria detection by the QBCTM test method is therefore more rapid than conventional blood film microscopy 1 3GnPRINCIPLESCOFGTHECPROCEDURES 1 3 10QBC oCentrifugalaHematologytTests The QBC Centrifuge is designed to spin up to 20 QBC blood tubes at the force time requirements needed to produce optimally packed cell layers for cell counting and analyses QBC blood tubes are 75 mm glass tubes internally coated with potassium oxalate acridine orange anticoagulants and an agglutinating agent The tubes are sealed with a plastic closure or rubber stopper and contain a precision molded float within the blood filled tube Rotor speed and timing of the centrifuge are computer controlled for maximum consistency in the formation of packed cell layers in soun QBC tubes 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 3 F 1 3 10QBC noCentrifugalaHematologytTests continued For technical assistance with QBC hematol
6. GLOADINGCANDcBALANCING The top lid is spring loaded and is automatically released when the Power switch of the centrifuge is off provided the Power Pack is on and connected to the centrifuge When a spin cycle is completed the latch releases after a momentary delay and the lid pops up Figure 4 2 Figure 4 2 Spring Loaded Lid Latch Mechanism Place tubes on the rotor in a balanced array with thectubecclosurescorustoppersc incE ZoPrep tubes cagainstcthecouternrimoofcthecrotor To maintain balance insert the tubes consecutively starting at slot 1 ae f When spinning an odd number of tubes e g three BE SURE TO BALANCE THE ROTOR BY PLACING AN UNFILLED QBC TUBE IN THE OPPOSITE SLOT e g 4 as shown in Figure 4 3 Use a closure or stopper and a float in the balance tube DT p Figure 4 3 Typical Placement of Blood and Balance Tubes Note If using an E Z Prep Tube for balancing do not attempt to re use the balance tube for a patient sample Re use for balancing only 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 11 T 4 3 TUBEDLOADINGCANDCBALANCINGG continued Before closing the lid INSTALL THE ROTOR COVER To secure the cover turn the cover knob clockwise onto the motor shaft Figure 4 4 Continue turning the knob until the cover
7. IREMENTS 00 0c cece eee s 15 6 4 CENTRIFUGATION INSTRUCTIONS sssseese eR hh hrs 16 6 4 1 Affixing Identification Labels anaana aaaea 16 6 4 2 Loading and Balancing Rotor sslsseseleeee hh 16 6 4 3 Centrifugation Procedures lssislslseseeeeees s 16 GAA Testing Tie s iex pEe ccu REX AG EF EnA ENEE eR RR URS ERG EE 17 Sectiono7OLIMITATIONSOOFGPROCEDURES sssssssesseele mere 18 7 1 QBC CENTRIFUGAL HEMATOLOGY TESTS ssesesee rrr 18 7 2 QBC MALARIA DETECTION TESTS sssslsessese ehh 18 Sectiono80CALIBRATIONOCHECKS suci tepe tanesi on hme 19 8 1 CHECKING ROTOR SPEED 00 c eect ttt 19 8 2 CHECKING TIMER ACCURACY 0 00 ccc tee 19 Sectiono9GMAINTENANCEGANDOCSERVICE 00 00 n 20 9 1 GENERAL CLEANING sra carre piii ven oe eee detained a x WE Eu ceu Ex ae ERE EE ow 20 9 2 ROTOR MAINTENANCE sss hen 20 9 2 1 Periodic Inspection sek sexe Rx RR weer ae m pue c deka Ric eee x a RE A 20 9 2 2 Rotor Cleaning Procedures mog sine en orto deen i nates Seinen ote Rug EE ENTRE E 21 9 3 RELEASING LID LATCH ise a code oe ea isie Se eee EErEE Ee EEIE eee ee RET 22 9 4 REPLACING POWER PACK FUSE 000 c i eei seh 22 9 5 TROUBLESHOOTING erra reatie DIM poke eae ee eee eee eae et a erbe se Ei a Bee s 23 9 6 PARTS UST ied Eme EIER TR eee a SE eI Ee EEE kee IER dx Ke Ra Ra 25 WARRANTY uaiziueaee dm pu era GUEST E RUE EG E YR ee
8. ND OTHERG APPROPRIATE BLOODo BARRIERD PROTECTION WHENG COLLECTING ANDGPREPARINGCBLOOD Be sure to clean the skin area with an antiseptic agent dry the area with a sterile wipe Puncture only with a sterile lancet Always use sterile needles and collection tubes 5 3 SPECIMENSCFORCQBC VCHEMATOLOGYCTESTS Venous blood for QBC hematology test analysis can be collected in Vacutainer brand tubes containing EDTA anticoagulant QBC tubes for venous blood are filled by means of a semi automatic pipetter supplied with the QBC System QBC capillary blood tubes are pre coated with anticoagulants and are filled with blood directly from a finger puncture After filling with blood the tubes are sealed with a rubber stopper or plastic closure The float in QBC tubes expands the buffy coat cell layers during centrifugation as previously described Stability For stability specifications consult the package insert of the the disposables in use in your laboratory 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 14 T 5 ABGSPECIMENSCFORCQBC CMALARIAGTESTS Collect capillary blood from the finger or foot directly into the malaria tube or draw venous blood and collect into the malaria tube Fill the tube with 55 to 65 uL of blood Malaria tubes are pre c
9. OTENTIALCBIOHAZARD CAPABLEX OF TRANSMITTINGG INFECTION c ALWAYSc WEARD GLOVESD AND OTHERo APPROPRIATED BLOOD BARRIERD PROTECTIONS WHENG COLLECTING ANDGPREPARINGCBLOOD WARNING QBC VHTUBESGARECMADEDCOFOGLASS GEXERCIS WHENo HANDLINGO ANDo PREPARINGO TUBES PREVENTGBREAKAGECANDCPOSSIBLECINJURY Consult Section 4 6 of this manual for detailed safety precautions IMPORTANT The coating ingredients in QBCTM tubes for hematology differ from those in QBC M tubes for malaria Accordingly do not interchange QBC Hematology Tubes with QBC Malaria Tubes since erroneous test results will be obtained 1 50SYMBOLODEFINITIONS Direct Current non alternating e K Not for use in the presence of flammable gases AN Attention Read the attached note 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 4 F Sectiono2 SYSTEMODESCRIPTION 2 10COMPONENTS The QBC Centrifuge is shipped with an external plug in power pack that supplies the required direct current D C voltage to power the motor
10. ROUND FLOAT while granulocytes the heaviest band form immediately above the packed red cells Figure 1 2 Cell layers in Centrifuged QBC E Z Prep Hematology Tube 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 2 F 1 2 10QBC noCentrifugalaHematologytTests continued Coated reagents in the QBC blood tube enhance the visual separation between cell layers and provide differential staining so that tubes can be read by non automatic and automatic QBC Analyzers 1 2 20QBC 0MalarianDetectionoTests The centrifugal test for detecting malaria parasites is based on a high precision glass capillary tube containing an equally precise plastic float When the blood filled tube is centrifuged at high speed for five minutes the float is buoyed by the packed red cells automatically positioning the float within the buffy coat layer between the packed red cells and the plasma During centrifugation blood cells in the buffy coat separate according to their densities forming visibly discrete bands Figure 1 3 Platelets constitute the topmost layer lymphocytes and monocytes the middle layer and granulocytes being the heaviest of the buffy coat cells concentrate immediately above the packed red cells Reagents coated in the QBC tube promote separation and metachromatic staining of these cells GRANULOCYTE LAYER LIGHT RED CELLS LYMPHOCYTE MONOCYTE
11. SPIN TIME DELAY lt 2 2 acd incre Cord teh IRI abe IIa ue boa awe cra Game Seedy 9 4 2 1 QBC Centrifugal Hematology Tubes sssslsessseesesl sr 9 42 2 QBG Malaria Tubes uu es duse dee en Ice oe ios sd i wid dab ORE ens 10 4 3 TUBE LOADING AND BALANCING ss RR RR RR RR aes 10 4 4 EMERGENCY UNLATCHING OF LID sseeee ee RR RR Ras 11 4 5 SPEED POWER LED MONITORS geri urune uere ete oleae he ee Sal aes ee Re Iur Mein Pub 11 4 6 PRECAUTIONS AND HAZARDS 00 00 ares 12 SectionoSoSPECIMENOCOLLECTION 0 0 hahaha 13 Bel GENERAL s iuc eiu led il Pei SL UU SE Ran E 13 5 2 HANDLING PRECAUTIONS 1S eid tete Ee he Lega vupe AE E EE dd ute dau 13 5 3 SPECIMENS FOR QBC HEMATOLOGY TESTS ssseeee RR Re ra 13 54 SPECIMENS FOR QBC MALARIA TESTS i i yexRRReR em Ee Ra EEIE EG een Rae ee mnes 14 ii 26423 QBC OpManual qxp 12 6 2006 11 23 AM Page iii T TablecofaContents continued Sectiono6O0CENTRIFUGATIONGPROCEDURES 000 cee lh her 15 6 1 MATERIALS PROVIDED i nia sdai aaka a cece a ATUR hon aora ii anioi A Ai 15 6 2 MATERIALS REQUIRED BUT NOT PROVIDED 0 0000 cent eeeae 15 6 2 1 QBC Centrifugal Hematology Tests 0 0c cena 15 6 2 2 QBG Malaria Wests icc rore ture vdd eux ren be dG sides Godew Dene dg odes ages 15 6 3 TEMPERATURE REQU
12. WX DOR eas e ERU AU AUR Ce Rune 26 ii 26423 QBC OpManual qxp 12 6 2006 11 23 AM Page iv F 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 1 F Sectiono1 INTRODUCTION 1 INTENDEDCUSE The QBC Centrifuge Figure 1 1 is an ultra compact instrument designed to spin down blood specimens for QBC Centrifugal Hematology tests or QBC Malaria tests Figure 1 1 QBC Centrifuge Model 425740 1 2z29UMMARYCANDCEXPLANATION 1 2 10QBC Centrifugal Hematology Tests QBC centrifugal hematology tests are based on a high precision glass capillary tube containing an equally precise plastic float When the tube is filled with blood sealed and centrifuged at high speed for five minutes the float is buoyed by the packed red cells automatically positioning the float within the buffy coat layer between the packed red cells and the plasma QBC tubes for hematology are produced in a standard design ie with an unassembled float and tube closure and in an E Z Prep tube design with a pre assembled float and a stopper WHITE CELLS LYMPHOCYTES PLATELET LAYER m MONOCYTES GRANULOCYTES Under centrifugation blood cells in the buffy coat layer separate according to their densities forming visibly discrete bands as shown in Figure 1 2 Platelets constitute the topmost band RED CELLS lymphocytes and monocytes the center band A
13. able cell layers in QBC blood tubes For stability be sure the unit rests on its suction feet Mark an envelope 300 mm 12 inches wider than the body of the centrifuge around the area in which the centrifuge is to be used Keep all personnel and hazardous materials outside this envelope when the centrifuge is operating 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 8 F 3 40 PLACEMENTCANDGOPERATINGGEQUIPMENTU continued WARNING IFo THISo EQUIPMENT ISo USEDo IN MANNE MANUFACTURER Su SPECIFICATIONS THE HAZARDSoTHISGDEVICEGPROVIDES INCONSISTENT WITHo THE PROTECTION MAYCBECIMPAIRED Be sure to keep the operating area within the temperatures listed below For QBC Centrifugal Hematology tests operating temperature should be maintained at 20 to 32 C 68 to 90 F to assure proper cell layering in QBC blood tubes For QBC Blood Parasite Detection tests operating temperature should be maintained at 16 to 37 C 60 to 99 F to assure the concentration of parasitic organisms 3 5 ROTORDASSEMBLY The the QBC Centrifuge is shipped with rotor assembly completely installed If the rotor is removed for example for cleaning see Section 9 2 it can be reinstalled a
14. acement fuse d Replace the fuse cover e Reconnect line cord of Power Pack 22 26423 QBC OpManual qxp 12 6 2006 11 26 AM Page 23 T 9 5 TROUBLESHOOTING A reference guide to common operating problems with the centrifuge is presented below PROBLEM CAUTION ACTION Fuse in Power Pack is blown See Section 9 4 and replace fuse Centrifuge shows no sign of power but primary electrical supply is available Loose power connection Check all power plugs Lid is not latched Close and latch lid Centrifuge does not operate when the ON button is pressed Failure of micro switch i Request service in latch mechanism q An electrical interlock prevents the lid from opening until rotor stops spinning Wait until all motion ceases Centrifuge rotor is spinning Centrifuge lid will not open but primary electrical supply Latch release delay is available Wait 4 5 s after rotor stops Plunger of latch solenoid See Section 9 3 is jammed and release solenoid plunger a Be sure tube load is properly balanced b Tighten rotor nut with wrench replace and tighten rotor cover Close top lid and re check for vibration c Check that surface does exceed 30 tilt limit Press power button OFF Centrifuge vibrates excessively to stop rotation Note momentary vibration is See Action steps normal during acceleration but should disappear upon Note Centrifuge is capable reaching full speed o
15. aning Agents The rotor assembly can be damaged by certain chemicals and disinfecting agents Clean and disinfect the rotor only with a solution containing a 1 10 dilution of commercial sodium hypochlorite 5 N CAUTION OTHER CLEANING ANDo DISINFECTINGS AGENTS c SINCE DANDCDAMAGECTHECROTOR CBECSURECTOCTHOROUGHLY ROTORGBEFORECRE INSTALLING A 1 10 dilution is prepared by adding one 1 part of household bleach to nine 9 parts of water Soak the rotor in the dilute bleach for atuleastttenc 10 min to destroy any viral and bacterial contaminants BE SURE TO REMOVE THE ROTOR PROMPTLY AFTER 10 MIN OF SOAKING IN THE BLEACH SOLUTION SINCE EXTENDED EXPOSURE TO THE BLEACH CAN CORRODE THE ROTOR b Rinsing and Drying After soaking in the dilute bleach solution specified above completely immerse the rotor in clean water Rinse again under running water to remove all traces of bleach Thoroughly dry the top and bottom surfaces of the rotor Oven drying may be used provided the temperature DOES NOT EXCEED 53 C 126 F IMPORTANT The motor shaft shaft pins and attaching hardware must also be dean and dry before reassembly If the rotor is cleaned without removing it from the centrifuge not a recommended procedure wipe it with a doth moisten
16. anual qxp 12 6 2006 11 23 AM Page 1 T p a oe E mo aE BEEN WENN vary tw Diagnostics QBC Centrifuge System Operator s Service Manual 26423 QBC OpManual qxp 12 6 2006 11 23 AM Page i T Bp Eb m 8H BEE wu tw Diagnostics QBC Centrifuge System Operator s Service Manual QBC E Z Prep are trademarks of QBC Diagnostics Inc Copyright O 2006 by QBC Diagnostics Inc and Vacutainer is a trademark of BD The QBC System is protected by one or more of the following U S Patent Numbers 4 027 660 4 082 085 4 007 396 4 159 896 4 156 570 4 091 659 4 141 654 4 137 755 4 181 609 4 209 226 4 190 328 4 558 947 4 567 754 4 594 165 4 683 579 4 695 553 and 4 779 976 as well as many foreign patents Other patents pending 4740 020 004 Rev A 0206 26423 QBC OpManual qxp 12 6 2006 11 23 AM Page ii T TablecofoContents Sections INTRODUCTION 22c3 442 c e8ni6a62008 cb 2S eere eed eI QUEE wiedeotenpeC coe Ee eret 1 1 1 INTENDED USE EE mee RR m Mid xxm x ERR Were y weld Seles Pee de eee ets 1 1 2 SUMMARY AND EXPLANATION 00000 RR RR RR RR RR RR res 1 1 2 1 QBC Centrifugal Hematology Tests 0 0 cece tte rn 1 1 2 2 QBC Malaria Detection Tests 0 ccc ee s 2 1 3 PRINCIPLES OF THE PROCEDURES Lss 25 2 ee ee Se aed na CS ieee rumeur ee ew es Mir ee ene x
17. circuit board and other internal systems of the instrument 2 2nCENTRIFUGECDESCRIPTION The QBC Centrifuge is a compact low profile unit measuring less than 13 centimeters 5 1 in high with cover down and housed in a sturdy plastic case As shown in Figure 2 1 the centrifuge incorporates a 20 place rotor mounted to the shaft of a brushless D C motor In addition to shock mounting of the motor to minimize vibration the centrifuge rests on four suction feet for added stability A protective metal cover which threads onto the motor shaft keeps the spinning tubes snugly in place on the rotor The hinged top lid with safety latch provides a housing or shield around the rotor during operation Electrical power to the centrifuge is controlled by a momentary ON OFF power button located on the front deck Before the motor will start the top lid must be closed and latched Once a spin cycle has started a safety interlock prevents the lid from being opened until the rotor brakes to a stop The spin cycle is controlled by an electronic timer on the main circuit board Spin time is fixed at 5 min Lid Latch Rotor Cover Lid Assembly Rotor Power Jack i va not shown Head Nut ai 1 nS ON OFF i Button Safety Interlock E Green Switch Dee Speed LED Yellow Power LED Figure 2 1 Features and Controls of QBC Centrifuge 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 5 T
18. d Power on lid latched 9 60PARTSOLIST The following spare parts and accessories for the QBC Centrifuge are available from your QBC System distributor or through the nearest QBC Diagnostics Inc office PartoDescription CataloguNo Formerly e Rotor Head 421289 42022001 Head Nut 421290 42022002 e Rotor Cover 421291 42022003 Head Nut Wrench 421292 42022004 Rotor Removal Wire 4277 020 006 e Line Cords Power Pack United States 421634 42432612 Europe 421551 42251012 United Kingdom 421554 42251015 Fuse 5A 2 pk 421565 42251026 Power Pack 421763 42474004 Operator s Manual QBC Centrifuge 422928 42474020 25 26423 QBC OpManual qxp 12 6 2006 11 26 AM Page 26 F WARRANTY QBC Centrifuge QBC Diagnostics Inc herein after referred to as QBC Diagnostics Inc warrants the QBC Centrifuge to be free from defects in workmanship and materials for a period of one 1 year from date of installation provided the centrifuge is operated in accordance with the Operator s Manual During such period QBC Diagnostics Inc agrees to replace or repair any parts which in its judgment are found to be defective provided the centrifuge has not been subjected to misuse or abuse The warranty stated herein shall extend to the original consumer and not to any subsequent consumer of the centrifuge QBC Diagnostics Inc shall not be liable for any incidental or consequential damages QBC Diagno
19. ed with water only DO NOT USE THE ABOVE DILUTE BLEACH SOLUTION Do NOT wipe the rotor with any other deaning agent since it cannot be sufficiently rinsed off while the rotor is installed 21 26423 QBC OpManual qxp 12 6 2006 11 26 AM Page 22 F 9 30RELEASINGOLIDGLATCH If the lid is latched and there is a loss of main power to the centrifuge i e a blown fuse in the Power Pack or interruption of primary electrical power the lid will remain latched A jammed plunger in the latch solenoid can also cause the lid to remain latched If you cannot wait for the restoration of power or the replacement of a fuse the lid can be opened as follows a Disconnect the Power Pack plug from the centrifuge b Turn the centrifuge upside down Using a Flat head screwdriver locate in the center the emergency lid open slot c Push gently on the end of the solenoid plunger until the latch is released Figure 9 1 9 2 Figure 9 1 Figure 9 2 d Turn the centrifuge upright Reconnect the plug of the Power Pack to the centrifuge e When the power problem is corrected check by closing and latching the lid then turn power on and off to verify that the lid unlatches properly 9 40REPLACINGDPOWERGPACKOFUSES a Unplug the line cord of the Power Pack from the wall receptacle b Remove the fuse cover from the Power Pack c Remove the blown fuse s and install a 5 amp repl
20. f 30 tilt without causing excessive vibrations 23 26423 QBC OpManual qxp 12 6 2006 11 26 AM Page 24 F 9 50 TROUBLESHOOTING continued Troubleshooting Guide for 425740 Centrifuge Power On Yellow Light Lid Latched Check Yellow Light Flashing Steady Check all Connections Turn Off Let Yellow Light Bleed Out Turn On Check Off On Switch Check Replace Lid Will Fuse Pop Open Close Firmly and Try Again Noisy Vibrates Lid Won t Open Remove and Reseat Rotor Cover Don t Overtighten Green Light On Or Flashing Latch Solenoid Wait for Problem Rotor to Stop Check Tube Balance Turn Off Let Yellow Light Bleed Out Turn On Lid May Now Pop Open Note Momentary vibration rumble is normal during acceleration and deceleration 24 26423 QBC OpManual qxp 12 6 2006 11 26 AM Page 25 F 9 50 TROUBLESHOOTING continued The combinations of On Off and Flashing status of the POWER and SPEED LED s shown below provide visual indicators of the current operating condition YellowoLED GreenoLED POWER SPEED CentrifugecStatus OperatomAction Flashing Ready to spin lid latched Press ON button On Off Power om NS Fete motion Load or unload tubes lid not latched On Flashin Power on lid latched Rotor is usually accelerating 9 rotomunderospeed to design speed On On Power on lid latched No action rotor at design spee
21. fuge is at design speed 12 000 80 rpm When the yellow light is on steadily but the green light is flashing the centrifuge is below but accelerating to design speed and when both lights are flashing the unit is above design speed If speed exceeds 14 800 rpm the centrifuge will automatically shut down A detailed table of LED Status Indicators is presented in Section 9 5 TROUBLESHOOTING 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 12 T 4 6CPRECAUTIONSCANDCHAZARDS The QBC Centrifuge is intended for in vitro diagnostic tests In order to obtain properly centrifuged specimens and to avoid damage or hazards the basic operating precautions below should be observed For smooth operation and long service life always place blood tubes on the rotor in a balanced array Always install the rotor cover before closing the top lid Never attempt to override the lid safety interlock Never attempt to pick up or move the centrifuge while the rotor is spinning Universal Precautions should be followed in handling all items contaminated with blood or other body fluids Exercise care when using QBC tubes The tubes are made of glass and may break possibly causing injury and exposure If a tube breaks carefully remove broken glass with a hemostat or other device using puncture resistant utility gloves and dispose of properly Disinfect rotor after a tube break See Section 9 2 2 For continued
22. g each between spin rest be sure the rotor cover is removed and the rotor empty 4 2 1nQBC Vn Hematologyc Tubes e f room temperature is 24 C or less lt 75 F wait 1 min between spins f room temperature is 25 28 C 75 82 F wait 3 min between spins e f room temperature is 29 30 C 84 86 F refer to the table below Between Between Between Between Above 5 Spins 1st amp 2nd Spins 2nd amp 3rd Spins 3rd amp 4th Spins 4th amp 5th Spins p f room temperature is 31 32 C 88 90 F wait 30 min between spins Note During QBC Hematology tests using standard or E Z Prep tubes room temperature should not exceed 32 C 90 P Important For room temperature specifications consult the package insert of the disposables in use in your laboratory 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 10 F 4 2 BETWEEN SPINCTIMECDELAYC continued 4 2 20QBC 2MalariacTubes e If room temperature is less than 33 C lt 91 F wait 1 min between spins f room temperature is 33 35 C 91 95 F wait 3 min between spins e f room temperature is 36 37 C 97 99 F With 1 to 5 consecutive spins wait 6 min between spins With consecutive spins above 5 wait 9 min between spins Note During QBC Malaria tests room temperature should not exceed 37 C 99 F 4 30 TUBE
23. he appropriate line cord and discard the unused cord 3 2nINSTALLATIONCANDCSERVICE For first time users of the QBC 7M System a representative of QBC Diagnostics Inc or your local QBC 7M System distributor will normally install the required equipment the installer will also train operating personnel in proper use of the equipment If necessary contact your nearest QBC Diagnostics Inc office to arrange for installation service Figure 3 1 QBC Centrifuge Showing Parts and Accessories Note Centrifuge is supplied with rotor installed 26423 QBC OpManual qxp 12 6 2006 11 24 AM Page 7 F 3 30CONNECTINGOPOWEROUNIT Be sure the Power Pack switch is OFF Insert the plug of the Power Pack output into the jack on the right side of the centrifuge Figure 3 2 Using the appropriate line cord connect the female plug to the receptacle in the side of the Power Pack Figure 3 3 connect the male plug to a grounded A C power receptacle then turn ON the Power Pack switch Failure to turn the Power Pack OFF before inserting the plug may result in an electrical arc which can damage the Power Pack or centrifuge When disconnecting the Power Pack from the centrifuge turn OFF the Power Pack switch and allowzatnleasto 30 cs before unplugging the output plug Failureotoowaitoatoleasto 30 osoafteroturningo thes Power Packo OFFo mayoresulto ino anc electricalo araiwhichocan damagecthecPowernPackoorcen
24. is snug DO NOT OVERTIGHTEN AN CAUTION NEVEROo ATTEMPT TOv CENTRIFUGE QBC TUBESC UNLESSG THEO COVERLIIS INSTALLEDO OVERO THEO ROTOR CSINCE TUBEX BREAKAGEU ANDo LOSSo OF SAMPLEGWILLOOCCUR V Figure 4 4 Tightening Knob of Rotor Cover t 4 4REMERGENCYCUNLATCHINGCOFCLID An electric interlock prevents the operator from manually opening the centrifuge lid once it is closed and latched In case the latched lid must be opened and the cover was installed over the rotor press the ON OFF button twice to start and stop the Centrifuge The lid will then open In case the latched lid must be opened but the rotor cover was inadvertently left off DOONOT attempt to start and stop the centrifuge with the ON OFF button instead turn off the Power Pack switch and wait for the yellow LED to turn off then turn on the Power Pack switch This will disengage the electric latch interlock and cause the lid to open Note For procedures on opening the lid in the event that main power to the centrifuge is lost through a fuse or main electrical failure see Section 9 3 4 55SPEED POWERECLEDCMONITORS The yellow POWER and green SPEED LED lights are used for monitoring the status of the centrifuge When both lights are on steadily the centri
25. izes at the design speed See Section 4 5 for sequencing of POWER and SPEED indicator lights AN CAUTION TOo PREVENTO PERSONALO INJURY DAMAGE CNEVERCLEANZON CPICKOUP TILTO THEO CENTRIFUGED WHILE IT SPEED x POWER VS OPERATING c NEVERO REMAIN WITHIN THEOCLEARANCEGENVELOPECDEFINEDGIN SECTIONC3 4COFGTHISCMANUALCLON GER THAN NECESSARY FOR OPERATIONALCREASONS Figure 4 1 Detail of ON OFF Button and POWER SPEED LED s Spin time is fixed at 5 min and is not adjustable After 5 min spin 15 20 s are required for the rotor to decelerate to a full stop at which time the lid latch unlocks To stop the centrifuge any time during a spin press and release the ON OFF button Note After completing any spin cycle full or interrupted the timer is automatically reset to zero 4 2GHBETWEEN SPINCTIMECDELAY Since the QBC test method depends upon proper cell layering in the hematology and malaria tubes blood sampled must be protected from excessive heat buildup in the rotor compartment which may adversely affect cell layer formation Accordingly between successive spins be sure to wait the appropriate time specified below IMPORTANT Durin
26. n in the QBC tube rack see Figure 6 2 or QBC work station Note See Test Timing in 6 4 4 Figure 6 2 Centrifuged Tubes Sorted in QBC Rack 6 4 4TestingoTime Testing of QBC Hematology tubes and Malaria tubes promptly after centrifugation is recommended The delays below however will not affect test results e QBC Hematology Tubes To obtain accurate cell counts test hematology tubes in the QBC Analyzer 4 hours after centrifugation Store the tubes vertically stopper down in the tube rack away from heat and intense light Consult the applicable QBC System Operator s Manual for test instructions e QBC Malaria Tubes After centrifugation malaria tubes can be held for as long as 3 days unrefrigerated at temperatures up to 37 C 99 F before microscopic examination Store tubes vertically closure down in the tube rack 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 18 T Sectiono7 LIMITATIONSCOFCPROCEDURES 7 10QBC oCENTRIFUGALGHEMATOLOGYCTESTS QBC tests should be performed at temperatures between 20 and 32 C 68 and 90 F If tubes are run at temperatures below 20 C test results may be erroneously high if tested above 32 C interfaces may become blurred and difficult to detect visually or automatically depending on the model of QBC Analyzer performing the test 7 20QBC OMALARIACDETECTIONGTESTS
27. oated with sodium heparin and dipotassium EDTA anticoagulants Stability Centrifuge the blood filled malaria tubes as soon as possible after filling See 6 4 4 for extended stability of malaria tubes prior to microscopic examination 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 15 T Sectiono6 CENTRIFUGATIONGPROCEDURES 6 10MATERIALSOPROVIDED The QBC Centrifuge is designed to spin up to 20 QBC type blood tubes over a fixed time of 5 min The centrifuge is supplied with a Power Pack and the test accessories listed in Section 3 1 6 20MATERIALSOREQUIREDoOBUTONOTOPROVIDED 6 2 10QBC oHematologyoTests In addition to a QBC Reader or Analyzer the following disposables and test accessories are required for QBC hematology tests e QBC E Z Prep Venous Tubes e Standard QBC Venous Tubes 100 Tests Cat No 424640 100 Tests Cat No 424240 e QBC EZ Prep Capillary Tubes 100 Tests Cat No 424245 100 Tests Cat No 424641 1000 Tests Cat No 424237 QBC EZ Prep Pipetter for Standard QBC Capillary Tubes venous blood Cat No 424225 100 Tests Cat No 424241 Work Station Cat No 424226 100 Tests Cat No 424246 Sterile wipes 1000 Tests Cat No 424238 With multi language instructions 6 2 2 QBC MalariacTests The following disposables and test acces
28. ogy tests contact 1 866 265 1486 QBC Diagnostics Inc 200 Innovation Blvd Suite 212 State College PA 16801 1 3 20QBC 0MalarianDetectionoTests The QBC Centrifuge is designed to spin up to 20 QBC malaria tubes at the force time requirements needed to cause the infected cells to concentrate in a narrow area of the tube for easy identification QBC malaria tubes are 75 mm glass capillaries internally coated with potassium oxalate acridine orange stain and anticoagulants Expansion of the separated cell layers is achieved with a plastic float which is inserted into the tube prior to centrifugation Rotor speed and timing of the centrifuge are computer controlled for consistent performance For malaria detection in the centrifuged sample the tube is examined under a fluorescence microscope in the region between the light red blood cells and granulocytes and lymphocytes monocytes where the parasites are most abundant For technical assistance with QBC malaria tests contact 1 866 265 1486 QBC Diagnostics Inc 200 Innovation Blvd Suite 212 State College PA 16801 1 40WARNINGSCANDoPRECAUTIONS Always observe good laboratory procedures when handling patient specimens WARNING BLOODu SPECIMENSo USEDo FORD THESE PROCEDURESO MAYO CONTAIN HEPATITISo Bo ANDG Go VIRUS o HUMANC IMMUNODEFICIENCYC VIRUSC HIV OTHERUINFECTIOUSCDISEASES CHANDLECBLOODPCASCACP
29. ormafternmany adjustmentso ort repairs check the rotor speed with a non contact strobe tachometer to assure that speed is sufficient to produce visibly distinct and differentiated cell layers in the QBC tube A window in the top lid and a reference line on the knob of the rotor cover are provided for speed checks with a non contact tachometer 8 2nCHECKINGCTIMERCACCURACY The electronic timer of the Centrifuge is designed to be accurate to 300 15 s Check the timer for accuracy against a reliable stopwatch or quartz timer at least every 30months as follows a Install the rotor cover b Close the latch and top lid c Press and release the ON button and simultaneously start the stopwatch d Stop the stopwatchowhennyoucaudiblyndetectocutoffiofipowerntonthesmotor and the centrifuge begins to decelerate e The stopwatch reading should be 5 min x 15 s If the 15 s tolerance is exceeded repeat the check to eliminate the possibility of procedural errors Request service if the accuracy check is unsatisfactory 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 20 F Sectiono9 MAINTENANCEDAND SERVICE 9 GENERALOCLEANING CAUTION TOOAVOIDOELECTRICALUHAZARDS OALWAYScDISCONNECT POWERLCCORDGBEFORECCLEANING Use soap or a mild detergent and water to clean
30. ove defects are found replace the rotor with a new assembly See Section 9 6 for catalog numbers Also inspect the motor shaft shaft pin and head nut for signs of wear corrosion or damage Replace any defective parts or request repair service Use the centrifuge only if all parts are in good condition 20 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 21 T 9 2 10Periodicalnspectiono continued To replace the rotor refer to Figures 3 4 and 3 5 Position the rotor hub on the motor shaft so that the slots on the underside of the hub are in line with the shaft pin Press the rotor down until the shaft pin is seated in the hub slots and 3 5 threads on the shaft protrude above the hub about 6 mm 4 Secure the rotor to the motor shaft with the head nut Lightly tighten the nut with the wrench Also inspect the centrifuge housing and lid for cracks missing pieces or deformations Extended service life of the rotor requires that the assembly be cleaned only by the procedures and with the approved agents specified in 9 2 2 Before using any cleaning or decontamination methods other than those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment 9 2 2nRotoraCleaningcProcedures To dean alwayscremovecthecrotormfromotheccentrifuge so that the procedure can be performed properly a Cle
31. s follows a Position the rotor so that the slots on the underside of the rotor hub are aligned with the drive pin in the motor shaft Figure 3 4 b Press the rotor down until the drive c Thread the head nut onto the shaft finger tight pin is seated in the hub slots and 3 5 threads of the shaft protrude above the hub about 6 mm 4 CAUTION TOG PREVENTO DAMAGE TOO ROTORO COVER ANDa CENTRIFUGES LID o BE SURED ROTORO IS CORRECTLYGSEATEDCONCDRIVEPPIN by turning it clockwise Tighten with a light torque using the head nut wrench supplied with the centrifuge Figure 3 5 DO NOT OVERTIGHTEN Turn nut 1 2 tube positions to tighten AGAINST protruding shaft threads e m Ea X N A Figure 3 5 Tightening Rotor with Head Nut Wrench 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 9 F Sectiono4 CENTRIFUGEGCOPERATION 4 10GENERALOPROCEDURES BeforecstartingothesQBC noCentrifuge cbecsurenthecrotoricovernisuinstalledoandotheclidoisoclosedoandolatched Theacentrifugeowillonotustartounlessctheclidcisolatched To start the 5 min spin press and release the ON OFF button on the front deck of the centrifuge Figure 4 1 The rotor will begin accelerating until it stabil
32. safety periodically inspect the rotor for signs of wear or defects as described in Section 9 2 of this manual Blood products and other biological materials should always be disposed of in accordance with laws regulations and institutional guidelines applicable to your facility Recommendations for Prevention of HIV Transmission in Health Care Settings MMWR 1987 36 Supplement 25 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 13 F Sectiono5 SPECIMENDGCOLLECTION 5 10GENERAL The QBC Centrifuge is designed for the preparation of specimen tubes for QBC Hematology and Malaria tests Detailed directions on blood collection preparation and stability for each type of test are contained in the package insert supplied with QBC hematology tubes and malaria tubes Handling precautions and collection procedures are summarized below 5 20HANDLINGOPRECAUTIONS e Exercise good laboratory procedures when collecting and handling patient specimens AN WARNING BLOODu SPECIMENSO USEDu FORO THESEO PROCEDURESOo MAYO CONTAIN HEPATITISc Bo ANDo VIRUS o HUMANG IMMUNODEFICIENCY1 VIRUSO HIV OTHERHINFECTIOUSCDISEASES CHANDLECBLOODCASCACPOTENTIALCBIOHAZARD CAPABLEX OFo TRANSMITTINGO INFECTION c ALWAYSo WEARD GLOVESO A
33. sories are required for QBC malaria detection tests QBC Malaria Kit Fluorescence Microscope or Standard 100 Tests Cat No 253037 White Light Microscope with ParaLens 2000 Tests Cat No 253005 Microscope Adapter Sterile wipes 6 3 TEMPERATUREGREQUIREMENTS For QBC Hematology Tests Optimum results are obtained when the laboratory temperature is maintained between 20 to 32 C 68 to 90 F To avoid excessive heating of blood tubes during spinning locate the centrifuge away from direct sunlight For Malaria Detection Tests Optimum results are obtained when test procedures are performed at temperatures between 16 to 37 C 60 to 99 F IMPORTANT For room temperature specifications Consult the package insert of the disposables in use in your laboratory 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 16 F 6 40CENTRIFUGATIONDINSTRUCTIONS N CAUTION DOo NOTO USEo THISO CENTRIFUGEO FORO SPINNINGO MICROHEMATOCRITo ORo OTHER CAPILLARYCTUBES CTHECROTORZANDOCOVERDAREODESIGNEDCTOCACCOMMODATEDONLY QBC TUBES OTHERO TYPESH OF CAPILLARIES MAYO BREAK o CAUSINGG BLOODz SPILLS AND3oLOSSCOFOSPECIMEN 6 4 10Affixingoldentifica
34. stics Inc makes no other warranties expressed or implied except as stated herein NationaloContact QBC Diagnostics 200 Innovation Blvd Suite 212 State College Pa 16801 USA Voice 814 231 7660 Fax 814 231 3118 Toll Free Technical Services 1 866 265 1486 Customer Service 1 877 231 3115 www qbcdiagnostics com 26
35. the lid housing and most parts of the QBC Centrifuge See below for special instructions on cleaning the rotor To prevent marring or scratching the finish avoid solvents or strong abrasives Dry all surfaces with soft paper towels or cloth Important When cleaning the inside of the centrifuge do not allow any fluids to run down into housing beneath the rotor Fluid in this area can damage the centrifuge Cleaning and decontamination may be necessary as a safeguard before the centrifuge or rotor is maintained repaired or transferred The user should attach a label or tag to the unit before it is moved or serviced certifying that cleaning and decontamination were performed 9 2hnROTORECMAINTENANCE 9 2 10Periodicainspection Remove and inspect the rotor for defects at least every 12 months and each time the rotor is removed for cleaning To remove the rotor a Turn the head nut counterclockwise with the wrench to remove b Insert the removal wire under the rotor Lift both sides of the wire to pry up the rotor Figure 9 1 Figure 9 1 Prying Rotor From Motor Shaft with Removal Wire Carefully inspect the rotor for defects that could impair its continued safe use Check top and bottom surfaces for the following Cracks especially hairline cracks in the region adjacent to the hub Surface corrosion particularly in the region of the hub Deep scratches or severe dents If any of the ab
36. tionoLabels MalariacTubescOnly Before Placing QBC malaria tubes on the centrifuge rotor a correctly marked patient identification label should be affixed on each tube between the two white lines scribed near one end of the tube Figure 6 1 Figure 6 1 Placement of ID Label on QBC Malaria Tube 6 4 20LoadingoandcBalancingoRotor Position QBC specimen tubes in the holder slots of the rotor with the stopper against the outer rim of the rotor Arrange the tubes in a balanced array i e 2 tube opposite 1 tube 4 opposite 3 etc When centrifuging an odd number of tubes balance the rotor with an empty QBC hematology tube or malaria tube Note Do not attempt to re use the balance tube for patient samples 6 4 30CentrifugationoProcedures a Secure the cover to the rotor then close and firmly latch the lid b Press and release the ON button to start the spin cycle c After the five min spin cycle is complete and the lid pops up open the lid and REMOVE THE TUBES IMPORTANT Failure to remove tubes promptly after centrifugation may affect cell layer boundaries and subsequent test results 26423 QBC OpManual qxp 12 6 2006 11 25 AM Page 17 F 6 4 30CentrifugationoProcedures continued d If not read promptly the centrifuged tube should be stored temporarily stopper or closure end dow
37. trifuge Figure 3 2 Connecting Output Cord Figure 3 3 Connecting AC Line Cord of Power Unit to Centrifuge Jack to Power Pack Plug CAUTION Too avoida electrical hazards ando equipment damage o connect thes power cord onlyctocgroundedoreceptaclescdeliveringathecvoltagecandofrequencynspecifiedoon theo datao plates off thea power supply o witho ao switcho ori other meansa ofa circuit disconnectioncl outsidenthenclearancecenvelopendefinedzinoSectionn3 4c0f this manual o Whena onlya ao 2 wirec receptaden and or anc unswitch inea is available c havec thes receptacle and or then supply lines modifi servicectechnician cindaccordancenwithcapplicablecelectricalocodes cto o thisonote 3 40PLACEMENTCANDCOPERATINGCEQUIPMENT Place the centrifuge on a smooth level working surface in a location where it will not be exposed to direct sunlight Centrifugations in direct sunlight can elevate the rotor compartment temperature and may adversely affect the formation of read

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