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Agilent 1260 Infinity Bio-inert High Performance Autosampler

1. Agilent Technologies Notices Agilent Technologies Inc 2011 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 65667 90000 Edition 03 2011 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate w
2. RT Device name based on the module HiP Autosampler Configuration Instrument 1 E Type ID based on the module product number Some Communication modules may allow changing the type based on Denice name HP Sampler hardware firmware This results in a change of features Type ID G5E87A and functions Sainai MES Serial number based on the module s Ea Firmware revision based on the module Seas Ls Options lists installed options Connection settings Options Syringe 100 pl Seat Cady 23 ob Max recon volume 100 00 pL Enema cortacts boad retaled ure BCD poat toe BOD port output lomat Thetmorter installed Fledble Cube installed Rinse valve nstaled Defne Welplates OK Carei Hep Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 5 61 5 Using the Module Main Screens of the Autosampler with Agilent Instant Main Screens of the Autosampler with Agilent Instant Pilot G4208A Below the main screens for the use of the autosampler are shown The Control screen allows System On Off System Get Ready System Clear Errors HIP ALS Wash needle HIP ALS SL 1280 wilinty GIETA LPO005 727 ogboo The System Info screen lists details of the autosampler Firmware revision On time Main Board information Transport assembly information Sampling unit information Syringe information Seal Wath Pume Solvent Selection Valve Hoctp BOOTP 0000 003003
3. Table9 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN Slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERM OSTAT for G2260A Prep ALS G1330B G1364B FC PS 2 Yes No Yes No Yes THERM OSTAT for G1364C FC AS G1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS Slaves G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 173 13 Hardware Information Table9 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWWD 2 No Yes Yes 1 Yes G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or
4. How to Achieve Higher Injection Volumes 69 How to Achieve High Throughput 71 How to Achieve Higher Resolution 72 How to Achieve Higher Sensitivity 75 How to Achieve Lowest Carry Over 76 This chapter gives hints on how to optimize the performance or use additional devices a Agilent Technologies 65 6 Optimizing Performance Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In gradient separations this volume causes a delay between the mixture changing in the pump and that change reaching the column The delay depends on the flow rate and the delay volume of the system In effect this means that in every HPLC system there is an additional isocratic segment in the gradient profile at the start of every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume isnot quoted even though this will have an effect on the chromatography This effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It isimportant therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often
5. The metering plunger has failed to move back to the home position The home position sensor on the sampling unit flex board monitors the home position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated Probable cause Suggested actions 1 Dirty or defective sensor Exchange the sampling unit flex board 2 Broken plunger Exchange the metering plunger and seal 3 Defective metering drive motor Please contact your Agilent service representative 4 Defective 1260 Infinity board Please contact your Agilent service representative M otor Temperature One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched off the motor to prevent damage to the motor See figure for motor identification Motor 0 temperature X axis motor e Motor 2 temperature Theta gripper rotation motor Motor 1 temperature Z axis motor The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Error Information 8 Probable cause Sugges
6. The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Short Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective flow sensor 2 Leak sensor incorrectly routed being pinched by a metal component Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 91 8 92 Error Information Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance acro
7. gt Do not connect the power cable to the Instrument while the covers are removed WARNING Incorrect line voltage at the module Shock hazard or damage of your instrument can result if the devices are connected to line voltage higher than specified gt Connect your module to the specified line voltage 24 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual CAUTION Site Requirements and Specifications 2 Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cabl
8. 1 specific Accessory kit 1 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Installing the Autosampler 3 Autosampler Accessory Kit Contents p n Description G5667 68755 Accessory kit bio inert G5667 60500 PEEK SST capillary bio inert 0 17 mm ID 400 mm long 5181 1519 CAN cable Agilent module to module 1 m 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 33 3 Installing the Autosampler Optimizing the Stack Configuration 34 If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Installing the Autosampler 3 Optimizing the Stack Configuration One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration See Figure 6 on page 35 and Figure 7 on page 36 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required Solvent cabinet Vacuum degasser Local User Interface Autosampler Column compartment Detector Figure6 Recommended Stack Configuration for 1260 Front View Ag
9. 1260 Infinity Bio inert High Performance Autosampler User M anual 147 10 Maintenance Replacing the M odule Firmware 148 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual This chapter provides information on parts material required for the module Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 11 Parts for Maintenance Overview of Maintenance Parts 150 Vial Trays 151 Recommended Plates and Closing Mats 152 Recommended Vial Plates 153 Accessory Kit 154 at Agilent Technologies 149 11 Parts for Maintenance Overview of Maintenance Parts For bio inert modules use bio inert parts only inert Item p n 1 0101 1416 G5611 21503 G5667 87200 G5667 87017 G5667 60310 G5667 60003 5068 0060 0100 1851 G5667 40500 5067 4695 5067 4131 oOo ae N Y OA A A UWV N p RF e O Description Rotor seal 3 grooves max 600 bar Piston seal bio inert Needle assembly bio inert Seat assembly bio inert 0 17 mm ID 100 mm Loop capillary bio inert Analytical head bio inert Bio inert stator head Stator face ceramic Tool for needle adjustment Sapphire piston 2 position 6port injection valve bio inert 150 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Vial Trays item p n G2258 60011 0515 0866 G1313 09101 0570 1574 G1329 60000 G1329 43200 61367 47200 7 64226 60021 no a A WD N me Parts for Maintenance 11 Vial
10. Agilent service representative defective Arm Movement Failed or Arm M ovement Timeout The transport assembly was unable to complete a movement in one of the axes The processor defines a certain time window for the successful completion of a movement in any particular axis The movement and position of the transport assembly is monitored by the encoders on the stepper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated See figure for axes identification e Arm Movement 0 Failed X axis Arm Movement 1 Failed Z axis Arm Movement 2 Failed Theta needle carrier rotation Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Defective motor assembly Please contact your Agilent service representative 4 Defective sample transport assembly Please contact your Agilent service representative flex board a 5 Defective main board ease contact your Agilent service representative Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 95 8 96 Error Information Valve to Bypass Failed The injection valve failed to switch to the bypass position The switching of the injection valve is monitored by two microswitches on the
11. Bus cable AC Power Figure9 Recommended Two Stack Configuration for 1260 Rear View 38 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Installing the Autosampler 3 Installing the Autosampler Parts required Hardware required Software required Description Autosampler Power cord Other cables see below and section Cable Overview on page 156 ChemStation and or Instant Pilot G4208A with the appropriate revisions see Table 3 on page 28 Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1 Place the Autosampler in the stack see Optimizing the Stack Configuration on page 34 2 Ensure t
12. Install the interface board Secure the screws Reconnect the cables to the board connectors F Screws 3 i f lt i Figure 26 Exchanging the Interface Board Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Replacing the M odule Firmware When Tools required OR OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or e if third part control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Agilent
13. carefull when you remove the needle carrier assembly gt Use the silicon safety tube supplied with every new needle 128 It is recommended to always exchange the needle assembly and the needle seat at the same time to prevent premature leakage Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Installing the needle assembly 1 Push the silicon safety tube delivered with every needle over the needle 2 Insert the loop capillary into the needle assembly and tighten the fitting hand tight 3 Pinch the holder clamp and reinsert the needle assembly into the needle carrier 4 Attach a 5 16 inch wrench to hold the position at the needle assembly Use a 1 4inch wrench to tighten the fitting of the loop capillary Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 129 10 Maintenance tgi lt F A ig g hivy installing tne needie assemp 5 Close the leak guide eh 7 When Rotating the tool to position the needle it has to be moved beyond the end point to get the correct position 6 To align the needle position the Positioning tool on the needle socket Ba ay Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the A
14. connected Agilent M odule to 3396 Series Il 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Identifying Cables 12 Agilent M odule to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low Oo 15 Se 3 3 Gray Start Low o NC 4 Blue Shut down Low O S NC 5 Pink Not connected NC 6 Yellow Power on High O g 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent M odule to Agilent 35900 A D Converters module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 161 12 Identifying Cables Agilent M odule to General Purpose p n 01046 60201 Pin Universal Pin Agilent SignalName Active module TTL 1 White Digital ground Taq 2 Brown Prepa
15. error is caused by an abnormal termination during the operation of the test whereas a failed result indicates that the test results were not within the specified limits Often it is only a damaged blank nut poorly shaped from over tightening that causes the test to fail Before investigating any other possible sources of failure make sure that the blank nut you are using is in a good condition and properly tightened Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Test Functions and Calibration 9 Sample Transport Self Alignment When Parts required Preparations The sample transport self alignment uses predefined positions on the well plate tray to calibrate the positioning of the needle The sample transport self alignment is required to compensate for larger deviations in positioning the needle carrier The sample transport self alignment is required after disassembling the system or when you exchange the sample transport the sampling unit the tray or the MTP main board This function is in the calibration screen of the Lab Advisor After disassembling the module or by larger deviations in the positioning of the needle Description 1 None Well plate tray needs to be installed and empty Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 109 9 Test Functions and Calibration Sample Transport Self Alignment 1 Run the Transport Alignment with the recommended user interfa
16. injection before switching it Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 69 6 Optimizing Performance How to Achieve Higher Injection Volumes i e injecting it onto an analytical column see Figure 13 on page 70 The valve can be conveniently located in the Thermostatted Column Compartment PumpA Autosampler Analytical column Position 1 Ta Detector __ Enrichment Pump B column Pump A Autosampler LL l Analytical column Position 2 Detector Enrichment Pump B column Figure 13 Sample enrichment 70 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Optimizing Performance 6 How to Achieve High Throughput The injection can be optimized for speed remembering that drawing the sample too fast can reduce the reproducibility Marginal gains are to be made here as the sample volumes used tend towards the smaller end of the range in any case A significant portion of the injection time is the time taken with the needle movements to and from the vial and into the flush port These manipulations can be performed while the previous separation is running This is known as overlapped injection and it can be easily turned on from the autosampler setup screen in the ChemStation Control Software The autosampler can be told to switch the flow through the autosampler to bypass after the injection has been made and then after for example 3 minutes into a 4 mi
17. lead to erroneous results The level of carryover is reported as the area of the peak in the blank solution expressed as a percentage of the area in the previous active injection The Agilent 1260 Infinity autosampler is optimized for lowest carryover by careful design of the flow path and use of materials in which sample adsorption is minimized A carryover figure of 0 002 should be achievable even when a triple quadrupole mass spectrometer is the detector Operating settings of the autosampler allow the user to set appropriate parameters to minimize carryover in any application involving compounds liable to stick in the system The following functions of the autosampler can be used to minimize carryover Internal needle wash External needle wash Needle seat backflush Injection valve cleaning The flow path including the inside of the needle is continuously flushed in normal operation providing good elimination of carryover for most situations Automated delay volume reduction ADVR will reduce the delay volume but will also reduce the flushing of the autosampler and should not be used with analytes where carryover might be a problem The outside of the needle can be washed using a wash vial in a specific location or the needle can be washed using the flush port If a wash vial ina tray location specified by the user is chosen then this vial should have no septum and should contain a solvent suitable for washing the sample fr
18. list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations Diagnostic Signals The module has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems see Diagnostic Signals 80 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Troubleshooting and Diagnostics 7 Status Indicators Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status _ S55 Figure 14 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 81 7 Troubleshooting and Diagnostics M odule Status Indicator The module status indicator indicates one of six possible module conditions When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator i
19. or H when between 2 0 V and 5 0 V with respect to the ground terminal Table 11 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation 178 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Hardware Information 13 Setting the 8 bit Configuration Switch The 8 bit configuration switch is located at the rear of the module Modules that do not h
20. phases to be assessed in an ordered scouting protocol This section considers how to get higher resolution with any chosen stationary and mobile phases If an automated method development system was used in the decision on phases it is likely that short columns were used for fast analysis in each step of the scouting Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Optimizing Performance 6 The resolution equation shows that the next most significant term is the plate count or efficiency N and this can be optimized in a number of ways N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle size and a longer column will give a higher plate number The pressure rises with the inverse square of the particle size and proportionally with the length of the column This is the reason that the 1260 Infinity LC system was designed to go to 600 bar so that it can run sub two micron particles and column length can be increased to 100 mm or 150 mm There are even examples of 100 mm and 150 mm columns linked to give 250 mm length Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1 4 What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure Methanol mixtures will generate more back pressure than acetonitrile mixtures Acetonitrile is often prefer
21. small step in the gradient The flow rate in use will also have an impact on the decision to use ADVR or not At 0 2 ml min the delay time saved is 21 seconds while at 1 0 ml min it is 4 seconds The ADVR function is unlikely to be suitable for applications involving compounds which are known to cause carry over problems Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Optimizing Performance 6 How to Achieve Higher Injection Volumes The standard configuration of the Agilent 1260 Infinity Autosampler includes a variable volume sample loop for up to 20 ul injections The metering device can inject a maximum volume of 40 ul and the sample loop cartridge can be exchanged to allow this The system delay volume due to the autosampler will increase accordingly Whenever a method is scaled down from a larger column to a smaller column it is important that the method translation makes an allowance for reducing the injection volume in proportion to the volume of the column to maintain the performance of the method This is to keep the volume of the injection at the same percentage volume with respect to the column This is particular important if the injection solvent is stronger more eluotropic than the starting mobile phase and any increase will affect the separation particularly for early running peaks low retention factor In some cases it is the cause of peak distortion and the general rule is to keep the injection s
22. the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 186 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Appendix 14 Some adjustments described in the manual are made with power supplied to the instrument and protec
23. tubing coming from the solvent bottle 5 Connect the wash port tubing to the upper tubing of the 6 Connect the tubing coming from the solvent bottle to the new cartridge use sand paper to get a good grip on the lower tubing of the new cartridge tubing 144 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Replacing Peristaltic Pump Cartridge 7 Push the cartridge onto the motor shaft until the clips 8 Reinstall the corrugated leak tubing click into place Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 145 10 Maintenance Installing the Interface Board When Parts required CAUTION At installation or when defective 1 Flat head screwdriver Description Interface board Electronic boards are sensitive to electrostatic discharge ESD and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 146 ur WN Be Switch OFF the autosampler at the main power switch Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the autosampler
24. usedwith mass spectrometric detection The delay volume in a system includes the volume in the pump from the pointof mixing connections between pump and autosampler volume of the flow path through the autosampler and connections between autosampler and column 66 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Optimizing Performance 6 How to Configure the Optimum Delay Volume For very fast gradients over 0 5 min the delay volume of the system can be easily reduced without changing the physical configuration of the system The change is achieved by changing the behavior of the autosampler The 80 ul delay volume of the autosampler is due to the flow path from the injection valve through the metering device needle needle seat and connecting capillaries back to the injection valve see Figure 12 on page 68 To make an injection the valve switches from mainpass to bypass so that the metering device can draw the sample into the needle capillary The injection is made when the valve switches back to mainpass and the sample is flushed onto the column The valve remains in this position during analysis so that the autosampler is continually flushed and hence the gradient has to flow through this delay volume to reach the column This can be eliminated by switching the injection valve from mainpass to bypass after the injection has been made and the injected sample has been flushed onto the column In practice this can b
25. 0 Infinity Bio inert High Performance Autosampler User M anual 97 8 Error Information Needle Carrier Failed The needle carrier on the Sample Transport Assembly failed to move correctly Probable cause Suggested actions 1 Defective Z motor Exchange the needle carrier assembly 2 Vial pusher blocked Please contact your Agilent service representative 3 Bad needle carrier positioning in X or Theta Exchange the needle carrier assembly 4 Defective vial pusher sensor Please contact your Agilent service representative 5 Defective 1260 Infinity main board Please contact your Agilent service representative Missing Vial or Missing Wash Vial No vial was found in the position defined in the method or sequence When the needle carrier moves to a vial and the needle goes into the vial the position of the needle is monitored by an encoder behind the vial pusher If no vial is present the encoder detects an error and the message missing vial is generated Probable cause Suggested actions 1 No vial in the position defined in the method Install the sample vial in the correct position or or sequence edit the method or sequence accordingly 2 Defective needle carrier assembly Exchange the needle carrier assembly 3 Defective transport assembly flex board Please contact your Agilent service representative 4 Defective 1260 Infinity board Please contact your Agilent service representative 98 Agilent 1260 Infinity B
26. 0 ul p n G5615 60018 and Max Light Cartridge Cell Bio inert 60 mm V o 4 0 ul p n G5615 60017 for Agilent 1290 Infinity Diode Array Detectors DAD G4212A B Bio inert flow cell 8 uL 20 bar pH 1 12 includes Capillary Kit Flow Cells BIO p n G5615 68755 p n G5615 60005 for Agilent 1260 Infinity Fluorescence Detector FLD G1321B Bio inert heat exchanger G5616 60050 for 1290 Infinity Thermostatted Column Compartment G1316C Bio inert Valve heads Bio inert Connection capillaries PEEK zirconium oxide sapphire PTFE PEEK fused silica PEEK fused silica PTFE PEEK steel cladded G4235A G5631A G5639A PEEK ceramic Al 03 based Upstream of sample introduction e Titanium Downstream of sample introduction e Agilent uses stainless steel cladded PEEK capillaries which keep the flow path free of steel and provide pressure stability to more than 600 bar For ensuring optimum bio compatibility of your Agilent 1260 Infinity Bio inert LC system do not include non inert standard modules or parts to the flow path Do not use any parts that are not labeled as Agilent Bio inert For solvent compatibility of these materials see Solvent information for parts of the 1260 Infinity Bio inert LC system on page 49 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Introduction 1 Overview of the Module The Autosampler transport mechanism uses an X Z theta r
27. 1BD33F TYPE 64720 65908 BIV AK_MIFG 72 SERN M 62 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual UsingtheModule 5 Main Screens of the Autosampler with Agilent Instant Pilot G4208A The Configure screen allows to configure Symbolic Name of module Volumes On Missing Vessel behaviour e Plate configuration e Flush Out Pump e Serial Interface configuration Sample Illumination Syringe pl Seat 1 2 4d Abort lt No Plate gt No Plate gt nl ae interface 19200 Baud 6 Bits None Parity Sample illumination OFF The Method screen lists all method parameters of the autosampler These can be edited Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 63 64 5 Using the Module Main Screens of the Autosampler with Agilent Instant Pilot G4208A The Maintenance screen allows EMF setup logging of maintenance activities e module identification blinking LED Firmware updates can be done via the System Maintenance screen The Diagnosis screen provides access to module specific tests e Injector steps Ceil test No Pass Fail result R IRL Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User K M anual 6 ee 6 e Optimizing Performance 8 Delay Volume and Extra Column Volume 66 Delay Volume 66 How to Configure the Optimum Delay Volume 67
28. 6539 Vial plate for 15 x 6 mL vials 1 pk 5022 6538 Vial plate for 27 Eppendorf tubes 1 pk Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 153 11 Parts for Maintenance essorvy Accessory Kit p n Description G5667 68755 Accessory kit bio inert G5667 60500 PEEK SST capillary bio inert 0 17 mm ID 400 mm long 5181 1519 CAN cable Agilent module to module 1 m 5063 6527 Tubing assembly i d 6 mm 0 d 9 mm 1 2 m to waste 154 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User K M anual 6 ee 12 e Identifying Cables Cable Overview 156 Analog Cables 158 Remote Cables 160 BCD Cables 163 CAN LAN Cables 165 External Contact Cable 166 Agilent Moduleto PC 167 Agilent 1200 M odule to Printer 168 This chapter provides information on cables used with the 1260 series of HPLC modules Apg Agilent Technologies 155 12 Identifying Cables Cable Overview 156 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EM C regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrato
29. 9 loop capillary change 113 M maintenance feedback 21 overview 123 150 positions 111 removing the needle assembly 125 replacing firmware 147 materials bio inert 11 message remote timeout 89 metering device change 114 needle carrier change 114 needle change 112 non operating altitude 27 non operating temperature 27 0 operating Altitude 27 Operating temperature 27 optimization achieving higher sensitivity 75 achieving higher resolution 72 achieving lowest carryover 76 columnuse 75 injection volumes 69 stack configuration 34 P packaging damaged 32 parts identification cables 155 physical specifications 27 power supply indicator 81 power considerations 24 power consumption 27 powercords 25 power switch 39 principle autosampler 15 R remote cable 160 repairs cautions and warnings 121 replacing firmware 147 resolution Optimization 72 RS 232C cable 167 communication settings 180 S safety class 186 safety information lithium batteries 189 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Index Safety general information 186 standards 27 symbols 188 sensitivity optimization 75 serial number information 184 shut down 89 site requirements powercords 25 solvent information 49 solvents 193 193 special interfaces 178 specification physical 27 stack configuration 38 rearview 38 Status indicator 82 steps injector 115 step commands 117 synchronizati
30. C 06 40 or with additional G1369C LAN Card G1316A CTCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No No AUX G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN Slaves THERM OSTAT for G1330A B NOT USED 174 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Hardware Information 13 The detector DAD M WD FLD VW D RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as interface to other modules LAN connector as interface to the control software e RS 232C as interface to a computer REMOTE connector as interface to other Agilent products Analog output connector s for signal output Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369A B LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software If an Agilent detector DAD MW D FLD VW D RID is in the system the LAN should be connected to the DAD MWD FLD VW D RID due to higher data load If no Agilent detector is part of th
31. Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability and is therefore the material of choice for biochemical instrumentation It is stable in the specified pH range and inert to many common solvents There is still a number of known incompatibilities to chemicals like chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc When used above room temperature it is sensitive to bases and various organic solvents which can cause PEEK to swell As normal PEEK capillaries are very sensitive to high pressure especially under such conditions Agilent uses stainless steel cladded PEEK capillaries in order to keep the flow path free of steel and to ensure pressure stability to at least 600 bar In case of doubt please consult common literature about the chemical compatibility of PEEK Titanium Titanium is highly resistant to oxidizing acids e g nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures which is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Reducing acids e g hydrochloric sulfuric and phosphoric acid can slightly corrode titanium which increases with acid concentration and temperature For examp
32. Rear view of the module Information on Instrument Serial Number The serial number information on the instrument labels provide the following information CCXZZ00000 Format cC Country of manufacturing DE Germany JP apan CN China X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module 00000 Serial number 184 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 14 Appendix General Safety Information 186 Lithium Batteries Information 189 The Waste Electrical and Electronic Equipment W EEE Directive 2002 96 EC 190 Radio Interference 191 Sound Emission 192 Use of Solvents 193 Agilent Technologies on Internet 194 This chapter provides addition information on safety legal and web gig Agilent Technologies 185 14 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure
33. Trays Description Tray for 2 plates 10 x 2 mL vials Screws for springs Spring Spring stud Tray base Adapter air channel Plug channel Tray for 100 micro vials Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 151 11 Parts for Maintenance Recommended Plates and Closing Mats Table 8 Recommended plates and closing mat Description Part Number Rows Columns Plate height Volume uL Package 384Agilent p n 5042 1388 16 24 14 4 80 30 384Corning No Agilent PN 16 24 14 4 80 384Nunc No Agilent PN 16 24 14 4 80 96 Agilent p n 5042 1386 8 12 14 3 500 10 96Agilent p n 5042 1385 120 96Agilent conical p n 5042 8502 8 12 17 3 150 25 96CappedAgilent p n 5065 4402 8 12 47 1 300 1 96Corning No Agilent PN 8 12 14 3 300 96CorningV No Agilent PN 8 12 14 3 300 96DeepAgilent31mm p n 5042 6454 8 12 31 5 1000 50 96DeepN unc31mm No Agilent PN 8 12 31 5 1000 96DeepRitter41mm No Agilent PN 8 12 41 2 800 96Greiner No Agilent PN 8 12 143 300 96GreinerV No Agilent PN 8 12 14 3 250 96Nunc No Agilent PN 8 12 14 3 400 Closing mat for all 96 Agilent plates p n 8 12 50 5042 1389 Using vessels higher than 41 mm will result in needle not being able to reach bottom of vessel 152 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Parts forMaintenance 11 Recommended Vial Plates p n Description G2255 68700 Vial plate for 54 x 2 mL vials 6 pk 5022
34. above mentioned recommendation a G1369A LAN card has to be used For installation and configuration see the G1369A documentation Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 45 4 LAN Configuration Connecting the module via LAN 46 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 5 Using the Module Preparing the Autosampler 48 Setting up the Autosampler with Agilent ChemStation 52 Control Settings 56 Method Parameter Settings 57 Module Configuration 61 Main Screens of the Autosampler with Agilent Instant Pilot G4208A 62 This chapter provides information on how to set up the autosampler for an analysis and explains the basic settings ee Agilent Technologies 47 5 Using the Module Preparing the Autosampler 48 Preparing the Autosampler For best performance of the autosampler When using the Autosampler in a system with a vacuum degassing unit shortly degas your samples before using them in the autosampler Filter samples before use in 1260 system Use High pressure filter kit p n 5067 4638 for inline filtering When using buffer solutions flush the system with water before switching it off Check the autosampler plungers for scratches grooves and dents when changing the piston seal Damaged plungers cause micro leaks and will decrease the lifetime of the seal Priming an
35. ach day prime the flush pump for three minutes with appropriate solvent When other measures have failed to eliminate carryover it might be that analyte is sticking inside the injector valve The injector valve can be set to make additional switching movements to clean out the flow path in the valve if problems occur here with carryover If the problem compounds need a high percentage of organic phase for elution it is recommended to switch the injection valve at the high percentage of organic phase after the last peak has eluted It is also recommended to switch the injection valve again after the initial conditions for the mobile phase have stabilized This ensures that the bypass groove in the rotor seal of the valve contains the gradient start conditions which is especially important for flow rates below 0 5 ml min For samples where the outside of the needle cannot be cleaned sufficiently with water or alcohol from the flush pump use wash vials with an appropriate solvent With an injector program several wash vials can be used for cleaning The optimum carry over performance of the Autosampler is achieved after a run in period of new instruments or after the exchange of consumable parts like needle needle seat and valve parts During injections in this period surfaces of these parts adjust to each other After this period we recommend back flushing the needle seat in order to get the sealing areas between needle and needle seat clean Re
36. apped inaction O AsPuro Nolet gt pi town man FR sl Injection Cheantens Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 57 5 Using the Module Setting up the Autosampler with Agilent ChemStation Injection M ode The settable Injection volume range is from 0 1 20 0 ul min Select to use Standard injection or Injection with Needle wash Needle wash Itis possible to select between using the built in flush port of the Autosampler or using a non capped vial Using needle wash is required to obtain minimum carry over An autosampler Stop Time can be set 58 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Using the Module 5 Setting up the Autosampler with Agilent ChemStation Injection Cleaning The Injection Valve Cleaning section allows you to specify the valve switching times at the end of overlap or sample flush Times 1 4 are the times when the valve switches to bypass for time 1 or to mainpass and bypass for times 2 3 and 4 The times must be specified in ascending order You can also switch the times to off Between the first and second and second and third valve switches a rinse is executed using the rinse volumes specified in the Injector Cleaning section Valve movements specifies the number of times that the valve switches from mainpass to bypass at times 2 3 and 4 in the field The maximum value is 2 default is 1 Agilent 1260 Inf
37. ass position If needle wash is required it will be done between step 7 and 8 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 15 1 Introduction Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position In this position the mobile phase flows through the autosampler metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over Sample loop Metoring Gavice Injection Rush us o needle port pa lt lt 2 gt lt gt gt 2 gt gt Peristaltic gt 2 pump To waste From pump Inpection valve To column Figure1 Mainpass Position When the sample sequence begins the valve unit switches to the bypass position Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Introduction 1 Autosampler Principle Figure2 Bypass Position The standard injection starts with draw sample from vial In order to do this the needle moves to the desired sample vial position and is lowered into the sample liquid in the vial to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle is then raised again and moved onto the seat to close t
38. ation 37 Installing the Autosampler 39 Flow Connections tothe Autosampler 41 Installation of stainless steel cladded PEEK capillaries 42 This chapter provides information on unpacking checking on completeness stack considerations and installation of the autosampler att Agilent Technologies 31 3 Installing the Autosampler Unpacking the Autosampler Damaged Packaging CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist 32 Ensure all parts and materials have been delivered with the autosampler For this compare the shipment content with the checklist included in each instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Table4 Autosampler Checklist Description Quantity Autosampler 1 Power cable 1 User manual on Documentation CD part of the shipment not module
39. ave their own LAN interface e g the TCC can be controlled through the LAN interface of another module and a CAN connection to that module Figure 29 Configuration switch settings depend on configured mode All modules without on board LAN default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 DIP 1 DOWN and DIP 2 UP allows special RS 232 settings for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 179 13 Hardware Information 180 The following tables represent the configuration switch settings for the modules without on board LAN only Table 12 8 bit Configuration Switch without on board LAN M ode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 Rsvo sys RsvD rsvo FC The LAN settings are done on the LAN Interface Card G1369A B Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compa
40. bove and the Thermostatted Column Compartment below see Optimizing the Stack Configuration on page 34 Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere 1 Open the front cover by pressing the button on the right side of the module 2 Install the capillary from the pump outlet into the port 1 of the injection valve 3 Install the capillary from the port 6 of the injection valve to the TCC The Autosampler can only be operated with the front and side covers closed Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 41 3 Installing the Autosampler Installation of stainless steel cladded PEEK capillaries Installation of Stainless Steel Cladded PEEK Capillaries CAUTION The Agilent 1260 Infinity Bio inert LC System uses PEEK capillaries which are cladded with stainless steel These capillaries combine the high pressure stability of steel with the inertness of PEEK They are used
41. ce for further information see Online Help of user interface ee eae Gira eas Vendy Cut an yogane wal pee tay ot rotabet Leones tat he te emety mage that he door chew Aip Pee vec wra ad ba ment iae me Figure 17 Sample Transport Self Alignment Running 110 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Test Functions and Calibration 9 Maintenance Positions Maintenance Positions Some maintenance procedures require the needle arm metering device and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the Agilent Lab Advisor Software the maintenance positions can be selected from the Tools icon When When performing Maintenance on the module Parts required Description 1 None Preparations None Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 111 9 Test Functions and Calibration Maintenance Positions 1 Run the Maintenance Positions with the recommended user interface for further information see Online Help of user interface g 1299 btety LC jorooooeoooj Figure 18 Maintenance Positions Running Change Needle The position is positioning the needle carrier so that there is easy access for changing needle or needle seat The position is to the far left and the current to the motors are off so that the arm ca
42. d Purging the System When the solvents have been exchanged or the system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel Therefore priming and purging of the system is required before starting an application Table5 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between Isopropanol Best solvent to flush air out of reverse phase and normal the system phase both times After an installation Ethanol or methanol Alternative to isopropanol second choice if no isopropanol is available To clean the system when Bidistilled water Best solvent to re dissolve using buffers buffer crystals After a solvent change Bidistilled water Best solvent to re dissolve buffer crystals Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual UsingtheModule 5 Solvent Information Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see pump manuals Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents which may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and reco
43. d above do not include any maintenance test functions e Screenshots used within these procedures are based on the Agilent Lab Advisor Software Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 83 7 Troubleshooting and Diagnostics Agilent Diagnostic Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues The Instrument Utilities is a basic version of the LabAdvisor wi
44. e done a few seconds after injection and is activated by selecting the Automatic Delay Volume Reduction ADVR function in the autosampler setup menu The Flush out Factor typically 5 times injection volume ensures that enough time is allowed to flush the sample out of the injector before switching to bypass This effectively reduces the system delay volume with 270 uL Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 67 6 68 Optimizing Performance 1 Valve in mainpass flow through 2 Valve in bypass drawing sample 3 Valve in bypass washing needle 4 Valve in mainpass sample injected p b ais aw Figure 12 Schematic of injection steps in 1260 Infinity Autosampler When using ADVR it should be noted that the gradient has already started at the pump at the instant of injection The question should be asked whether the gradient has already reached the autosampler in which case a small step in the gradient will result This happens when the delay volume is less than the flush out volume and is not necessarily a problem but may be a factor to be considered in a method transfer With a flush out factor of 5 and an injection volume of 10 ul the autosampler will allow 50 ul to pass through before switching to bypass which with a delay volume of 50 ul means the gradient just reached the injection valve Smaller injection volumes will have no effect but for larger injection volumes this will introduce a
45. e system the LAN interface should be installed in the pump or autosampler Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 175 13 Hardware Information RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 10 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI 176 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Hardware Information 13 Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 3 TX DTR 4 4 DTR GND 5 i 5 GND DSR 6 6 DSR RTS 7 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 D89 DB9 DB9 Male Female Female Male Figure 28 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board APG Remote The APG R
46. e tasks Parts required p n Description 1 01080 83202 Blank nut Preparations Solvents must be present in both channels 1 Run the System pressure test with the recommended user interface for further information see Online Help of user interface Genel Umts Soros Test Name System pressure test for Aladdin Description Preiminary system presare teat tor Asan Mockde G 220A LP00000003 Approx Time Net defines Satus Passed Test Procecken Feet Prepare purp pressure inst System leak 21 ber Emer the test pressure Flush e system System checking leak rate of pump lesen Wark mut ip op System checking leak rate of system Evaluate redta Wee Oe Restore system corhourabor Figure 15 System Pressure Test Result Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 107 9 Test Functions and Calibration System Pressure Test b Pump head leak test on Enter the test pressure Figure 16 System Pressure Test Dynamic pressure input System Pressure Test Evaluation System Pressure Test Failed Probable cause Suggested actions 1 Pump leakages Perform the Pump Head Leak test 2 Loose or leaky fittings Tighten the fittings or replace capillaries 3 Autosampler leakages Perform the Autosampler Leak test 4 Thermostatted Column Compartment valve Replace the TCC valve rotor seal NOTE 108 leakages Notice the difference between error in the test and a failed result An
47. e the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 27 Firmware Update M echanism Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 171 13 Hardware Information 172 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A Xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com EN US SUPPORT DOWNLOADS FIRMWARE Pages LC aspx Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Hardware Information 13 Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces
48. emStation or other 1260 Infinity modules refer to the corresponding documentation Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual UsingtheModule 5 Setting up the Autosampler with Agilent ChemStation GD Creer SEQUENCE W 120s vrcotar s warns 2 crnvowns QB onevovir_ ists Gover scs conse s I nsreerrs a xe vrests P earo vos Gh LCEESENFORELS S G uesgroR mwo s T ccreseqyror_pip s GB ueeegron wwo s W Lovorest s ah roeusts 192 168 254 11 0 00 0 00 E 6 on off proumes m socs Sun CRRYOVER TEST F cons Lesaton Sampe Name Samp info Ota o DWD feat Tire Ci data Je test Tests F RAG 120 5 a irs og Method and Run Control oS8eeesses Figure 11 ChemStation Method amp Run Control After successful load of the ChemStation you should see the module as an active item in the graphical user interface GUI Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 53 5 Using the Module Catina un tha Aut ynnler wi A etting up tne Autosampie ith Agile The Autosampler User Interface Injection Volume 0 0 uL Sample Location Edit Wellplate Types 25 Assign Wellplates Within the Autosampler user interface there are active areas If you move the mouse cursor across the icons tray EMF button the cursor will change and you may click on the icon to Turn on off the autosampler 1 Configure the sample
49. emature leakage Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 125 10 Maintenance Removing the needle assembly 1 Inthe user interface start the maintenance mode and select Change needle seat function In the Agilent Lab Advisor software the Change needle seat function can be found in the Tools section 2 Open the front door and remove the side door 3 Turn the Needle carrier 90 clockwise 126 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Removing the needle assembly 5 Attach a 5 16 inch wrench to hold the position at the 6 Pinch the holder clamp pull back and remove the loop needle assembly Use a 1 4 inch wrench to loosen the capillary from the needle assembly fitting of the loop capillary Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 127 10 Maintenance Installing the needle assembly When Tools required Parts required Preparations When the limit in the needle into seat counter in the EM F is exceeded or when needle shows indications of damage blockage or leaks p n Description 8710 0510 Y4 inch wrench p n Description 1 5667 87200 Needle assembly bio inert In order to avoid leaks close the shutoff valves in the pump or remove tubings from solvent bottles Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be
50. emote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 177 13 Hardware Information input load is 2 2 kOhm against 5 0 V and output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high
51. es Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 25 2 Site Requirements and Specifications Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The module dimensions and weight see Table 2 on page 27 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench should carry an Agilent system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and all
52. ew of Maintenance 123 Cleaning the module 124 Removing the needle assembly 125 Installing the needle assembly 128 Exchanging the Needle Seat 131 Replacing the Rotorseal 134 Removing the metering seal 138 Installing the metering seal 141 Replacing Peristaltic Pump Cartridge 143 Installing the Interface Board 146 Replacing the Module Firmware 147 11 Parts forMaintenance 149 Overview of Maintenance Parts 150 Vial Trays 151 Recommended Plates and Closing Mats 152 Recommended Vial Plates 153 Accessory Kit 154 12 Identifying Cables 155 Cable Overview 156 Analog Cables 158 Remote Cables 160 BCD Cables 163 CAN LAN Cables 165 External Contact Cable 166 Agilent Module to PC 167 Agilent 1200 Module to Printer 168 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Contents Contents 13 Hardware Information 169 Firmware Description 170 Interfaces 173 Setting the 8 bit Configuration Switch 179 Electrical Connections 183 14 Appendix 185 General Safety Information 186 Lithium Batteries Information 189 The Waste Electrical and Electronic Equipment W EEE Directive 2002 96 EC 190 Radio Interference 191 Sound Emission 192 Use of Solvents 193 Agilent Technologies on Internet 194 8 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User K Manual e ee l e Introduction J Features 10 Bi
53. finity Bio inert High Performance Autosampler User M anual 101 8 Error Information Peristaltic Pump Error The peristaltic pump motor in the autosampler has failed The current on the motor is used by the MTP board to monitor the speed of the peristaltic pump motor If the current falls below a certain value the error message is generated Probable cause Suggested actions 1 Defective motor Please contact your Agilent service representative 2 Defective SUD board Please contact your Agilent service representative 3 Defective 1260 Infinity main board Please contact your Agilent service representative Vessel or Wash Vessel Error The needle does not reach the target position in the vial or in the vessel of the well plate The sensor behind the vial pusher in the needle carrier assembly detects the successful completion of the needle movement to the vessel If the needle fails to reach the end point the sensor fails to recognize the needle movement and the error message is generated Probable cause Suggested actions 1 Bad vessel definition in the plate Check the vessel definition in the plate configuration configuration 2 Closing mat to rigid thick Check that the closing mats is not too thick 3 Bad X or Theta positioning Exchange the needle carrier assembly 4 Defective encoder on the needle carrier Please contact your Agilent service assembly representative 102 Agilent 1260 Infinity Bio inert High Performance Au
54. for HPLC applications PTFE PTFE polytetrafluorethen Teflon is inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Sapphire Ruby and Al 03 based ceramics Sapphire Ruby and ceramics based on AlgO3 are inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Data above were collected from external resources and are meant as a reference Agilent cannot guarantee for the completeness and correctness of such information Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually increases at higher temperatures In case of doubt additional resources should be consulted Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 51 5 Using the Module Setting up the Autosampler with Agilent ChemStation 52 The setup of the Autosampler is shown with the Agilent ChemStation B 04 02 SP1 1260 Depending on the controller e g Agilent Instant Pilot EZChrom Elite the screens look different For the Instant Pilot refer to Main Screens of the Autosampler with Agilent Instant Pilot G4208A on page 62 This section describes the autosampler settings only For information on the Agilent Ch
55. gilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 93 8 Error Information Module Error Messages These errors are autosampler specific Exhaust Fan Failed The exhaust fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain value the error message is generated and the module shuts down Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Front Door Error The front door and or the SLS board are damaged Probable cause Suggested actions 1 The sensor on the SLS board is defective Please contact your Agilent service representative 2 The door is bent or the magnet is Please contact your Agilent service misplaced broken representative 94 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Error Information 8 Side Door Error The side door and or the main board are damaged Probable cause Suggested actions 1 The door is bent or the magnet is Please contact your Agilent service representative misplaced broken 2 The sensor on the main board is Please contact your
56. gular Preventive Maintenance service is recommended as the carry over performance of the Autosampler depends on the integrity of these consumable parts Using the G4227A Flexible Cube will additionally improve the carry over performance and life time of these parts Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 77 6 Optimizing Performance How to Achieve Lowest Carry Over 78 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 7 Troubleshooting and Diagnostics Overview of the M odule s Indicators and Test Functions 80 Status Indicators 81 Power Supply Indicator 81 Module Status Indicator 82 User Interfaces 83 Agilent Diagnostic Software 84 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces ot Agilent Technologies 79 7 Troubleshooting and Diagnostics Overview of the M odule s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a
57. he power switch on the front of the module is OFF switch stands out Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 39 3 Installing the Autosampler Installing the Autosampler 3 Connect the power cable to the power connector at the rear of the module lea ope REMOTE RS 232 CAN CAN CAN DC OUT C haier Technologies Prod No RMA aaaea t ae Te e Made in Germar Figure 10 Rearview of Autosampler 4 Connect the CAN cable to other Agilent 1260 Infinity modules 5 Connect the APG remote cable optional for non Agilent instruments 6 Turn on the power by pushing the button at the lower left hand side of the module The power button stays pressed in and the status LED should be green NOTE When the line power button stands out and the green light is off the module is turned off NOTE The module was shipped with default configuration settings For changing these settings please refer to service manual Setting the 8 bit configuration switch 40 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Installing the Autosampler 3 Flow Connections to the Autosampler Parts required Preparations Description System Capillaries and tubing from Accessory Kit Autosampler is installed in system This procedure shows the Autosampler outside of a system In an Agilent 1260 Infinity Liquid Chromatograph the Autosampler is located between a Pump a
58. he sample loop In case of an injector program several steps are interspersed at this point Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 17 1 Introduction Autosampler Principle lt a a Peristaltic pump d P From pump To column Figure3 Drawing the Sample Flush the Needle Before injection and to reduce the carry over for very sensitive analysis the outside of the needle can be washed in a flush port located behind the injector port on the sampling unit As soon as the needle is on the flush port a peristaltic pump delivers some solvent during a defined time to clean the outside of the needle At the end of this process the needle returns to the injection port 18 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Introduction 1 Autosampler Principle gt Flush the needle Figure Inject and Run The final step is the inject and run step The six port valve is switched to the main pass position and directs the flow back through the sample loop which now contains a certain amount of sample The solvent flow transports the sample onto the column and separation begins This is the beginning of a run within an analysis In this stage all major performance influencing hardware is flushed internally by the solvent flow For standard applications no additional flushing procedure is required Agilent 1260 Infinity Bio inert High Performance Autosampler Use
59. her end depends on the instrument to which connection is being made Agilent M odule to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog ed 158 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent M odule to BNC Connector Identifying Cables 12 p n 8120 1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog SIO E A Center Center Analog Agilent M odule to General Purpose p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog Red Analog Pai ae Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 159 12 Identifying Cables Remote Cables 160 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent M odule to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent SignalName Active module TTL Z 9 1 White Digital ground a NC 2 Brown Prepare run Low 3 3 Gray Start Low z NC 4 Blue Shut down Low k NC 5 Pink Not connected ee NC 6 Yellow Power on High Oo 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not
60. ids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl Ox gt 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF Use of Solvents with Agilent 1260 Infinity Bio inert LC systems Observe the following recommendations on the use of solvents with Agilent 1260 Infinity Bio inert LC systems Brown glass ware can avoid growth of algae Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Do not use solvents or samples which are not compatible with materials in the flow path see in this manual Bio inert materials and Solvent information for parts of the 1260 Infinity Bio inert LC system Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 193 14 Appendix Agilent Techno
61. ilent 1260 Infinity Bio inert High Performance Autosampler User M anual 35 3 Installing the Autosampler Optimizing the Stack Configuration Remote cable CAN Bus cable to local user interface CAN Bus cable Analog detector signal 1 or 2 outputs per detector AC power LAN to LC ChemStation location depends on detector Figure7 Recommended Stack Configuration for 1260 Rear View 36 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Installing the Autosampler 3 Optimizing the Stack Configuration Two Stack Configuration To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 8 on page 37 and Figure 9 on page 38 Instant Pilot Detector Column compartment Autosampler Thermostat for the ALS optional Solvent cabinet Degasser optional Pump Figure8 Recommended Two Stack Configuration for 1260 Front View Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 37 3 Installing the Autosampler Optimizing the Stack Configuration LAN to control software CAN Bus cable to Instant Pilot Thermo cable TI i NNN optional Z NN LLS Remote cable CAN
62. in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual In This Guide In This Guide This manual covers the Agilent 1260 Infinity Bio inert High Performance Autosampler G5667A 1 Introduction This chapter gives an introduction to the autosampler an overview of the instrument and a description of internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Autosampler This chapter provides information on unpacking checking on completeness stack considerations and installation of the autosampler 4 LAN Configuration This chapter provides information on connecting the autosampler to the Agilent ChemStation PC 5 Using the Module This chapter provides information on how to set up the autosampler for an analysis and explains the basic settings 6 Optimizing Performance This chapter gives hints on how to optimize the perfo
63. in the high pressure flow path after sample introduction needle seat capillary through the TCC heat exchangers to the column Such capillaries need to be installed carefully in order to keep them tight without damaging them by overtightening Strong force torque will damage SST cladded PEEK capillaries Be careful when installing stainless steel cladded PEEK capillaries gt See Agilent 1260 Infinity Bio inert Quaternary LC System M anual for correct installation 42 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual ee 4 LAN Configuration Setting up the module ina LAN environment 44 Connecting the module viaLAN 45 This chapter provides information on connecting the autosampler to the Agilent ChemStation PC ee Agilent Technologies 43 4 LAN Configuration Setting up the module in a LAN environment It is not recommended to connect an Agilent 1260 Infinity system via the G5667A Autosampler The detector is producing the most data in the stack followed by the pump and it is therefore highly recommended to use either of these modules for the LAN connection 44 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual LAN Configuration 4 Connecting the module via LAN If the G5667A Autosampler is being operated as a standalone module or if a connection via LAN is required regardless of
64. information 190 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Appendix 14 Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 191 14 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A At Operator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test 192 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Appendix 14 Use of Solvents Observe the following recommendations on the use of solvents Brown glass ware can avoid growth of algae Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic ac
65. inity Bio inert High Performance Autosampler User M anual 59 5 Using the Module g up the Autosampler with Ag Injection Program xj The pretreatment injector program comprises a series of Q HEALS QD HPALS Injector Program numbered lines each specifying an operation that the Sone eae autosampler carries out sequentially When you activate a pretreatment injector program it replaces the standard Function Parameter injection cycle r Select Append to add the contents of the edit line to the end of the table Select Insert to insert the contents of the edit line above the currently selected line Select Delete to delete the currently selected line Select Clear All to clear all pretreatment injector program functions from the table Select M ove up to move the currently selected line one position up in the order of execution Select M ove down to move the currently selected line one position down in the order of execution Select Cut to delete the currently selected line and place it on the clipboard Select Copy to copy the currently selected line to the clipboard Select Paste to paste the line on the clipboard at the current position 60 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Using the M odule Setting up the Autosampler with Agilent ChemStation Module Configuration These settings are available via menu Instrument gt More 1260 Infinity ALS gt Autosampler Configuration
66. injection volume kit hardware modification required Up to 1500 uL with multiple draw hardware modification required Typically lt 0 25 RSD from 5 100 uL Typically lt 0 5 RSD from 2 5 uL Typically lt 0 7 RSD from 1 2 uL volume M easured with injections of caffeine in water 1 10 uL n 10 Up to 600 bar 8700 psi 0 2 5cp 2 x well plates MTP 10 x 2 0 mL vials 108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 mL in 2 x 27 Eppendorf tube plates Typically lt 17 s using the following standard conditions Default draw speed 100 uL min Default eject speed 100 uL min Injection volume 5 ul Typically lt 0 004 For measurement conditions see 1 2 3 1 13 short term 14 Optional with G1330B 4 40 C Upstream of sample introduction e titanium gold PTFE PEEK zirconium oxide Downstream of sample introduction e PEEK zirconium oxide Early maintenance feedback EMF electronic records of maintenance and errors Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Site Requirements and Specifications 2 Table3 Specifications Agilent 1260 Infinity Bio inert High Performance Autosampler G5667A Type Specification Communications Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number
67. io inert High Performance Autosampler User M anual Initialization Failed Error Information 8 The autosampler failed to complete initialization correctly The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined routine During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause 1 Side door not installed correctly 2 Sample transport sampling unit not aligned correctly 3 Mechanical obstruction 4 Defect 5 Defect 6 Defect 7 Defect ive sampling unit flex board ive transport assembly flex board ive sampling unit motor ive 1260 Infinity main board Suggested actions Check if the side door is installed correctly Check if the magnet is in place in the side door Do an auto alignment Ensure unobstructed movement of the transport assembly P P P P ease cont ease cont representat ease cont representat ease cont representat Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual act your Agi representati ve ve ve ve act your Agi act your Agi act your Agi ent servi ent servi ent servi ent servi 99 8 100 Error Information Metering Home Failed
68. ion The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up gt e WARNING DAI alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 188 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Appendix 14 Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same o
69. ions The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization One analog output provides signals for integrators or data handling systems The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 witha line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EM C regulations Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 183 13 Hardware Information Electrical Connections Rear view of the module E lea aa THERMO REMOTE AN CAN DC OUT YE tatonnen G Cem y Product No AIMA Sai No Figure 30
70. ists as they are known for optimum inertness towards biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals like iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction does not contain metals at all Table1 Bio inert Materials Module Materials Agilent 1260 Infinity Bio inert Quaternary Pump Titanium gold platinum iridium G5611A zirconium oxide ruby PTFE PEEK Agilent 1260 Infinity Bio inert High Performance Autosampler Upstream of sample introduction G5667A e Titanium gold PTFE PEEK zirconium oxide Downstream of sample introduction e PEEK zirconium oxide Agilent 1260 Infinity Bio inert M anual Injector PEEK zirconium oxide G5628A Agilent 1260 Infinity Bio inert Analytical Fraction Collector PEEK zirconium oxide PTFE G5664A Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 11 1 12 Introduction Table1 Bio inert Materials Module Materials Bio inert Flow Cells Standard flow cell bio inert 10 mm 13 ul 120 bar 12 M Pa for MWD DAD includes Capillary Kit Flow Cells BIO p n G5615 68755 p n G5615 60022 for Agilent 1260 Infinity Diode Array Detectors DAD G1315C D Max Light Cartridge Cell Bio inert 10 mm V o 1
71. k 35 twostack 37 D defect on arrival 32 delay volume 66 delivery checklist 32 Diagnostic software 84 dimensions 27 electrical connections descriptions of 183 electrostatic discharge ESD 146 EM F early maintenance feedback 21 error messages fan failed 93 rear blind seat missing 103 error messages arm movement 95 autosampler 94 compensation sensoropen 92 compensation sensor short 92 front doorerror 94 initialization failed 99 invalid vial position 101 leak sensor open 91 leak sensor short 91 leak 90 metering home failed 100 missing vial 98 motor temperature 100 needle lock failed 97 needle to needle seat position 97 peristaltic pump error 102 195 Index remote timeout 89 shut down 89 synchronization lost 90 timeout 88 valve to bypass failed 96 valve to mainpass failed 96 vessel error 102 vessel stuck to needle 103 external contact cable 166 extra column volume 66 F fan failed 93 firmware description 170 main system 170 resident system 170 update tool 171 updates 171 147 upgrade downgrade 147 frequency range 27 G general error messages 88 H humidity 27 injection volume achieving higher volumes 69 injector Steps 115 installation bench space 26 power considerations 24 instrument layout 22 interfaces 173 196 internet 194 L LAN cable 165 leaksensoropen 91 leak sensor short 91 leak 90 line frequency 27 line voltage 27 lithium batteries 18
72. l Introduction 1 Autosampler Principle The movements of the autosampler components during the sampling sequence are monitored continuously by the autosampler processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence is not completed successfully an error message is generated Solvent is bypassed from the autosampler by the injection valve during the sampling sequence The needle moves to the desired sample vial position and is lowered into the sample liquid in the vial to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle is then raised again and moved onto the seat to close the sample loop Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The standard sampling sequence occurs in the following order The injection valve switches to the bypass position The plunger of the metering device moves to the initialization position The needle lock moves up The needle moves to the desired sample vial position The needle lowers into the vial The metering device draws the preset sample volume The needle lifts out of the vial The needle is then moved onto the seat to close the sample loop woonrnroauw Fr WN The needle lock moves down 10 The injection cycle is completed when the injection valve switches to the mainp
73. l inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User K M anual 6 ee 2 e Site Requirements and Specifications Site Requirements 24 Physical Specifications 27 Specifications 28 This chapter provides information on environmental requirements physical and performance specifications Agg Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the module Power Consideration The module power supply has wide ranging capabilities and accepts any line voltage in the range mentioned in Table 2 on page 27 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover
74. le as it might deform the seal screws firmly and have the tag on the right side looking from the front Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 141 10 Maintenance Installing the metering seal 3 Press the piston into the seal 4 Reinstall the metering device to the sampling unit by tightening the two fixing screws alternately with a 4 mm hex key 5 Connect the two capillaries to the metering device using a Next Steps 1 inch wrench 6 Close the front door 7 In the user interface exit the Change M etering device function and exit the maintenance mode In the Lab Advisor software the Change Metering device function can be found in the Tools section 142 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Replacing Peristaltic Pump Cartridge When Tubing blocked or broken Parts required p n Description 1 5065 4445 Peristaltic pump cartridge The peristaltic pump cartridge is a replaceable unit The tubing inside the pump is not replaceable 1 Remove the corrugated leak tubing 2 Press the two clips on the front of the peristaltic pump cartridge Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 143 10 Maintenance Replacing Peristaltic Pump Cartridge 3 Pull the cartridge forward off the motor shaft 4 Disconnect the tubing leading to the wash port and the
75. le the corrosion rate of 3 HCl about pH 0 1 at room temperature is about 13 um year At room temperature titanium is resistant to concentrations of about 5 sulfuric acid about pH 0 3 The addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates Titanium is subject to corrosion in anhydrous methanol which can be avoided by adding a small amount of water about 3 Slight corrosion is possible with ammonia gt 10 Fused silica Fused silica is inert against all common solvents and acids except hydrofluoric acid It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH larger than 12 the use of flow cells with sapphire windows is recommended Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual UsingtheModule 5 Gold Gold is inert against all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia a mixture of concentrated hydrochloric and nitric acid Zirconium Oxide Zirconium Oxide ZrOg is inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert against almost all common acids bases and solvents There are no documented incompatibilities
76. le with connecting printers and plotters It s also called Null M odem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 167 12 Identifying Cables Agilent 1200 M odule to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control M odule 168 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 13 Hardware Information Firmware Description 170 Interfaces 173 Overview Interfaces 175 Setting the 8 bit Configuration Switch 179 Communication Settings for RS 232C 180 Special Settings 182 Electrical Connections 183 Rear view ofthe module 184 Information on Instrument SerialNumber 184 This chapter describes the autosampler in more detail on hardware and electronics ot Agilent Technologies 169 13 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections anon instrument specific section called resident system an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series
77. leaning the module 124 Removing the needle assembly 125 Installing the needle assembly 128 Exchanging the Needle Seat 131 Replacing the Rotor seal 134 Removing the metering seal 138 Installing the metering seal 141 Replacing Peristaltic Pump Cartridge 143 Installing the Interface Board 146 Replacing the M odule Firmware 147 This chapter describes the maintenance of the Autosampler Agg Agilent Technologies 119 10 Maintenance Introduction to Maintenance Introduction to Maintenance Metering device Leak tubing Sampling unit Loop capillary Transport assembly Needle carrier Needle Needle flush port Tray Power switch Needle seat Injection valve Figure 25 on page 120shows the main user accessible assemblies of the autosampler These parts can be accessed from the front simple repairs and don t require to remove the autosampler from the system stack Needle wash peristaltic pump 120 Figure 25 Main user accessible assemblies Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Warnings and Cautions WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the metal top cover of the module No serviceable parts inside gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent i
78. logies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 194 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Index Index 8 8 bit configuration switch without On Board LAN 179 A accessory kit 33 154 Agilent Diagnostic software 84 Agilent Lab Advisor software 84 Agilent Lab Advisor 84 Agilent oninternet 194 algae 193 193 ambient operating temperature 27 ambient non operating temperature 27 analog signal 177 analog cable 158 apgremote 177 arm 113 position 113 automated delay volume reduction 76 battery safety information 189 BCD cable 163 bench space 26 bio inert 122 materials 11 blank nut 107 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual C cable analog 158 BCD 163 CAN 165 connecting APG remote 38 connecting CAN 38 connecting GPIB 38 connecting LAN 38 connecting the ChemStation 38 connecting the power 38 external contact 166 LAN 165 remote 160 RS 232 167 cables analog 156 BCD 156 CAN 157 LAN 157 overview 156 remote 156 RS 232 157 CAN cable 165 carryover 76 cautions and warnings 121 cleaning 124 Communication settings RS 232C 180 compensation sensor open 92 compensation sensor short 92 condensation 26 configuration one stac
79. ls A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation Agilent 1260 Infinity Bio inert High Performance Autosampler U
80. mmendations in subsequent sections Solvent information for parts of the 1260 Infinity Bio inert LC system For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials see Bio inert Materials on page 11 in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness towards biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals like iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction does not contain metals at all However there are no materials which combine suitability for versatile HPLC instrumentation valves capillaries springs pump heads flow cells etc with complete compatibility to all possible chemicals and application conditions This section gives recommendations which solvents should be preferred Chemicals which are known to cause issues should be avoided or exposure should be minimized e g for short term cleaning procedures After using potentially aggressive chemicals the system should be flushed with compatible standard HPLC solvents Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 49 5 50 Using the Module PEEK PEEK
81. modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like run synchronization through APG remote error handling e diagnostic functions or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 170 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Hardware Information 13 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files from a USB Flash Disk Agilent LabAdvisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or us
82. n In the Agilent Lab Advisor software the Change metering device function can be found in the Tools section 138 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Removing the metering seal 3 Remove the two attached capillaries with a 1 4 inch wrench 4 Unscrew alternately the two fixing screws with a 4 mm hex key 5 Pull the metering device away from the sampling unit Y 1 TTS 6 Remove the two fixing screws at the base of the metering device Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 139 10 Maintenance Removing the metering seal 7 Remove the head body 8 Using the piston carefully remove the metering seal Clean the chamber and ensure all particular matter is removed 140 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Installing the metering seal When When poor injection volume reproducibility or when metering device is leaking Tools required p n Description 8710 0510 Y4 inch wrench 8710 2392 4mm Hex key Parts required p n Description 1 G5611 21503 Piston seal bio inert Preparations Removing the metering seal see Removing the metering seal on page 138 1 Install the new metering seal Press it firmly into position 2 Reassemble the metering device M ake sure to tighten Avoid any offset ang
83. n be turned while servicing the module Change Needle Fred crit Figure 19 Maintenance Positions Change Needle 112 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Test Functions and Calibration 9 Change Loop Capillary The Change Loop Capillary command positions the arm in the middle of the tray at half height to enable easy exchange of the loop cartridge Change Loop Capillary Figure 20 Maintenance Positions Change Loop Capillary Arm Position The home position of the autosampler ensures a better access to the tray area and for exchanging trays When transporting the module it is highly recommended to use the Park Arm command in order to place the Arm ina position for safe transport Arm Position Figure 21 Maintenance Positions Arm Position Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 113 9 Test Functions and Calibration Change Needle Carrier The Change Needle Carrier function moves the needle to the front of the autosampler enabling easy access to the needle carrier mechanism Change Needle Carrier Figure 22 Maintenance Positions Needle Carrier Start moves the needle to the front of the sample tray area End resets the autosampler after the needle carrier has been changed Change Metering Device When removing the metering device is necessary by exchanging the metering seal for instance the metering drive needs to be moved to a posi
84. ndicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running e An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis A red blinking modules with on board LAN or yellow blinking modules without on board LAN indicator indicates that the module is in resident mode e g during update of main firmware A fast red blinking modules with on board LAN or fast yellow blinking modules without on board LAN indicator indicates that the module is in boot loader mode e g during update of main firmware In such a case try to re boot the module or try a cold start 82 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Troubleshooting and Diagnostics 7 User Interfaces Depending on the user interface the available tests and the screens reports may vary Preferred tool should be the Agilent Diagnostic Software see Agilent Lab Advisor software The Agilent ChemStation B 04 02 an
85. njections Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 123 10 Maintenance Cleaning the module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid could drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 124 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Removing the needle assembly When When the limit in the needle into seat counter in the EM F is exceeded or when needle shows indications of damage blockage or leaks 8710 0510 inch wrench Parts required p n Description 1 65667 87200 Needle assembly bio inert Preparations In order to avoid leaks close the shutoff valves in the pump or remove tubings from solvent bottles WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be carefull when you remove the needle carrier assembly gt Use the silicon safety tube supplied with every new needle It is recommended to always exchange the needle assembly and the needle seat at the same time to prevent pr
86. nk value tq time length of gradient or segment of gradient min F flow ml min Vi column delay volume A B change in fraction of solvent B during the gradient S constant ca 4 5 for small molecules This shows that k and hence resolution can be increased by having a shallower gradient 2 to 5 min change is a guideline higher flow rate and a smaller volume column This equation also shows how to speed up an existing gradient if the flow is doubled but the gradient time is halved k remains constant and the separation looks the same but happens in half the time Recently published research has shown how a shorter STM column at temperatures above 40 C can generate higher peak capacity than a longer STM column by virtue of running it faster Refer to Petersson et al J Sep Sci 31 2346 2357 2008 Maximizing peak capacity and separation speed in liquid chromatography Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Optimizing Performance 6 How to Achieve Higher Sensitivity The sensitivity of a separation method is linked to the choice of stationary and mobile phases as good separation with narrow peaks and a stable baseline with minimal noise are desirable The choice of instrument configuration will have an effect and a major impact is the setup of the detector This section considers how sensitivity is affected by Pump mixer volume Narrower columns De
87. nutes run to start the process of aspirating the next sample and preparing for injection This can typically save 0 5 to 1 minute per injection Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 71 6 Optimizing Performance How to Achieve Higher Resolution 72 Increased resolution in a separation will improve the qualitative and quantitative data analysis allow more peaks to be separated or offer further scope for speeding up the separation This section considers how resolution can be increased by examining the following points e Optimize selectivity Smaller particle size packing Longer Columns Shallower gradients faster flow Resolution between two peaks is described by the resolution equation 1 m a 1 k 1 yN F 4 a k Rs where R resolution N plate count measure of column efficiency a selectivity between two peaks k retention factor of second peak formerly called capacity factor The term that has the most significant effect on resolution is the selectivity a and practically varying this term involves changing the type of stationary phase C18 C8 phenyl nitrile etc the mobile phase and temperature to maximize the selectivity differences between the solutes to be separated This is a substantial piece of work which is best done with an automated method development system which allows a wide range of conditions on different columns and mobile
88. o inert M aterials 11 Overview ofthe Module 13 Autosampler Principle 15 Early Maintenance Feedback 21 Instrument Layout 22 This chapter gives an introduction to the autosampler an overview of the instrument and a description of internal connectors gg Agilent Technologies 1 Introduction Features 10 The Agilent 1260 Infinity Bio inert High Performance Autosampler features an increased pressure range up to 600 bar enabling the use of today s column technology sub two micron narrow bore columns with the Agilent 1260 Infinity LC System Increased robustness is achieved by optimized new parts high speed with lowest carry over by flow through design increased sample injection speed for high sample throughput increased productivity by using overlapped injection mode and flexible and convenient sample handling with different types of sample containers such as vials and well plates Using 384 well plates allows you to process up to 768 samples unattended For specifications see Specifications on page 28 This 1260 Infinity Autosampler has been introduced together with the Agilent 1260 Infinity Liquid Chromatograph Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Introduction 1 Bio inert Materials For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials in the flow path also referred to as wetted parts which are widely accepted by life scient
89. obot to optimize the positioning of the sampling arm on the well plate Once the sampling arm is positioned over the programmed sample position the programmed sample volume is drawn by the metering device into the sampling needle The sampling arm then moves to the injection position where the sample is flushed onto the column The Autosampler employs a vial plate pusher mechanism to hold down the vial or the plate while the needle is drawn back from the sample vessel a must in case a septum is used This vial plate pusher employs a sensor to detect the presence of a plate and to ensure accurate movement regardless of the plate used All axes of the transport mechanism x z theta robot are driven by stepper motors Optical encoders ensure the correct operation of the movement The standard metering device provides injection volumes from 0 1 100 uL The entire flow path including the metering device is always flushed by the mobile phase after injection for minimum internal carry over An additional needle flush station with a peristaltic pump is installed to wash the outside of the needle This reduces the already low carry over for very sensitive analysis The bottle containing the mobile phase for the wash procedure will be located in the solvent bottle cabinet Produced waste during this operation is channeled safely away through a waste drain The six port only 5 ports are used injection valve unit is driven by a high speed hyb
90. odular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 165 12 Identifying Cables External Contact Cable 5 gt Ea oO 4l 10 6 55 e e An One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent M odule Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 A Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 166 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Identifying Cables 12 Agilent M odule to PC p n Description G1530 60600 RS 232 cable 2 m RS232 61600 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatib
91. olvent the same or weaker than the starting gradient composition This has a bearing on whether or by how much the injection volume can be increased and the user should check for signs of increased dispersion wider or more skewed peaks and reduced peak resolution in trying to increase the injection size If an injection is made in a weak solvent then the volume can probably be increased further because the effect will be to concentrate the analyte on the head of the column at the start of the gradient Conversely if the injection is in a stronger solvent than the starting mobile phase then increased injection volume will spread the band of analyte down the column ahead of the gradient resulting in peak dispersion and loss of resolution Perhaps the main consideration in determining injection volume is the diameter of the column as this will have a big impact on peak dispersion Peak heights can be higher on a narrow column than with a larger injection on a wider column because there is less peak dispersion With 2 1 mm i d columns typical injection volumes might range up to 5 to10 ul but it is very dependent on the chemistry of the analyte and mobile phase as discussed above In a gradient separation injection volumes of about 5 of the column volume might be achieved whilst maintaining good resolution and peak dispersion One way to achieve larger injections is to use a trapping column selected by a switching valve to capture and concentrate the
92. om the needle The septum is not used to avoid wiping contamination off the needle on the downstream only to re apply it on the upstroke The needle can be dipped into the vial multiple times This will be effective in removing a small degree of carryover but for more effective washing of the outside of the needle use the flushport The flush port is located above and behind the needle seat and a peristaltic pump delivers the wash solvent It has a volume of 0 68 ml and the peristaltic pump delivers 6 ml min which means the flush port volume is completely Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Optimizing Performance 6 refilled with fresh solvent in 7 s If the flush port is selected the user can set how long the outside of the needle is to be washed with fresh solvent This may be as low as two or three seconds in routine situations where carryover is less of a problem and 10 to 20 s for more complete washing It is recommended that washing the outside of the needle in the flush port should be standard procedure to avoid contaminating the needle seat If the needle seat becomes contaminated it will have to be back flushed by manually changing the flow connections to clean it This is one of the tasks that can be automated using the Flexible Cube module The flush port and its solvent delivery pump and tubing should be regularly flushed to ensure the lowest carryover For example before using the system e
93. on lost 90 system setup and installation optimizing stack configuration T temperature sensor 90 test functions 80 timeout 88 troubleshooting error messages 87 80 status indicators 80 81 V vial trays 151 voltage range 27 Ww warnings and cautions 121 weight 27 34 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 197 www agilent com In This Book This manual contains technical information about the Agilent 1260 Infinity Bio inert High Performance Autosampler G5667A e introduction and specifications e installation using and optimizing troubleshooting and diagnose maintenance parts identification hardware information safety and related information Agilent Technologies 2011 Printed in Germany 03 2011 R067 80000 G5667 90000 oa Agilent Technologies
94. output Safety features Leak detection and safe leak handling low voltages in maintenance areas error detection and display 1 Chromatographic conditions Column Agilent ZORBAX SB C18 2 1 x 50 mm1 8 um p n 827700 902 mobile phase A 0 1 TFA in water B 0 1 TFA in acetonitrile isocratic B 35 flow rate 0 5 mL min temperature 30 C UV detection Sample 1200 ng pl chlorhexidine dissolved in mobile phase A 1 uL injected and measured on G4212A DAD 10 mm cell Wavelength 257 nm 4 ref 360 nm 16 slit 4 nm 10 Hz MS detection Sample 50 ng ul chlorhexidine dissolved in mobile phase A 1 uL injected and measured on Agilent 6460 QQQ in specified conditions MRM 1 505 5 170 CE 36 V MRM 2 505 5 201 2 CE 20 V fragmentor 150 V delta EM V 200 V For solvent compatibility refer to section Solvent information for parts of the 1260 Infinity Bio in ert LC system in the manual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 29 2 Site Requirements and Specifications Specifications 30 Agilent 1260 Infinity Bio inert High Performance Autosampler User Manual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 3 Installing the Autosampler Unpacking the Autosampler 32 Damaged Packaging 32 Delivery Checklist 32 Autosampler Accessory Kit Contents 33 Optimizing the Stack Configuration 34 One Stack Configuration 35 Two Stack Configur
95. ow it to warm Slowly to room temperature to avoid condensation 26 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Site Requirements and Specifications 2 Physical Specifications Table 2 Physical Specifications Type Specification Comments Weight 15 5 kg 35 Ibs Dimensions height x 200 x 345 x 440 mm 8 x 13 5 x 17 inches width x depth Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 300 VA 200 W 683 BTU Maximum Ambient operating 4 55 C 41 131 F temperature Ambient non operating 40 70 C 4 158 F temperature Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards IEC CSA Installation Category I Pollution Degree 2 For indoor use only UL Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 27 2 Site Requirements and Specifications Specifications 28 Table3 Specifications Agilent 1260 Infinity Bio inert High Performance Autosampler G5667A Type Specification Injection range Precision Injection Accuracy Pressure range Sample viscosity range Sample capacity Injection cycle time Carry over pH range Sample cooling Materials in flow path GLP features 0 1 100 uL in 0 1 uL increments Up to 40 uL with reduced
96. r M anual 19 1 20 Introduction Autosampler Principle Figure 5 Inject and Run Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Introduction 1 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EM F Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or t
97. r equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 189 14 Appendix The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 s This product complies with the W EEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the W EEE Directive Annex this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more
98. re run Low o0 DE KEY 3 Gray Start Low oo lie 4 Blue Shut down Low am 5 Pink Not connected oOo 20 6 Yellow Power on High Ss O 18 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 162 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Identifying Cables 12 BCD Cables jas m i nt pn 60000 I O LEGGE COCOJT JA XN FN One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent M odule to General Purpose module Green 1 BCD 5 20 Violet 2 BCD 7 80 i m E Black 5 BCD 0 1 ay Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 not connected 14 notconnected 15 5V Low Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 163 12 Identifying Cables Agilent M odule to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent SignalName BCD Digit module 1 1 BCD 5 20 2 2 BCD7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCDO 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 164 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual CAN LAN Cables Identifying Cables 12 Both ends of this cable provide a m
99. red because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better selectivity certainly for small molecules less than about 500 Da The viscosity can be reduced by increasing the temperature but it should be remembered that this can change the selectivity of the separation Experiment will show if this leads to increase or decrease in selectivity As flow and pressure are increased it should be remembered that frictional heating inside the column will increase and that can lead to slightly increased dispersion and possibly a small selectivity change both of which could be seen as a reduction in resolution The latter case might be offset by reducing the temperature of the thermostat by a few degrees and again experiment will reveal the answer The van Deemter curve shows that the optimum flow rate through an STM column is higher than for larger particles and is fairly flat as the flow rate increases Typical close to optimum flow rates for STM columns are 2 ml min for 4 6 mm i d and 0 4 ml min for 2 1 mm i d columns Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 73 6 74 Optimizing Performance In isocratic separations increasing the retention factor k results in better resolution because the solute is retained longer In gradient separations the retention is described by k in the following equation pr o F 100 A B V S where k mea
100. rid stepper motor During the sampling sequence the valve unit bypasses the autosampler and connects flow from the pump to the column directly During injection and analysis the valve unit directs the flow through the autosampler which ensures that the entire sample is injected onto the column and that the metering unit and needle are always free from sample residue before the next sampling sequence begins Control of the vial plate temperature in the thermostatted autosampler is achieved using an additional Agilent 1290 Infinity Series module the Agilent Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 13 1 Introduction 1290 Infinity Series thermostat for ALS FC Spotter The thermostat contains Peltier controlled heat exchangers A fan draws air from the area above the sample vial tray of the autosampler It is then blown through the fins of the cooling heating module There it is cooled or heated according the temperature setting The thermostatted air enters the autosampler through a recess underneath the special designed sample tray The air is then distributed evenly through the sample tray ensuring effective temperature control regardless of how many vials are in the tray In cooling mode condensation is generated on the cooled side of the Peltier elements This condensed water is safely guided into a waste bottle for condensed water 14 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anua
101. ritten agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 J une 1995 oras a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 J une 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 J une 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 J une 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result
102. rmance Autosampler User M anual 5 55 5 Using the Module Setting up the Autosampler with Agilent ChemStation Control Settings These settings are available via right click on the Active Area of the ALS GUI xj Missing Vessel The handling of missing vessels can be Missing Vessel confi gu red Illumination Can be turned on off Linked Pump To configure which pump delivers flow to the Autosampler Prime Flush Pump Priming the Needle wash flush pump Linked Pump G42204 DE92900137 X Prime Flush Pump off D on for Ok Cancel Help 56 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual UsingtheModule 5 Setting up the Autosampler with Agilent ChemStation M ethod Parameter Settings These settings are available via Menu gt Instrument gt Setup Agilent 1260 Infinity Autosampler or via right click on the Active area NOTE The signal window in the lower part is not shown when opening the parameter settings via right mouse on the Autosampler user interface F Method of G56674 LP0005572 Bie x HIP Sampler G5667A gammy Misciion Mode Advanced 7 r brgre hon vo mn 50 a Akay ed Standard injection Draw speed 2000 slan Ingnenen with neede wath Eject speed 200 smn Dima position ao m aeaieo Equibeaton tite 20 sc Sample flush ot factor 50 tenes injection vohane Viel Well bottom rening High roughest Matome delay voke reduc ton Stoptime Poiti Enable overl
103. rmance or use additional devices 7 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 3 In This Guide 8 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 9 Test Functions and Calibration This chapter describes the tests for the module 10 Maintenance This chapter describes the maintenance of the Autosampler 11 Parts for Maintenance This chapter provides information on parts material required for the module 12 Identifying Cables This chapter provides information on cables used with the 1260 series of HPLC modules 13 Hardware Information This chapter describes the autosampler in more detail on hardware and electronics 14 Appendix This chapter provides addition information on safety legal and web Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Contents Contents Introduction 9 Features 10 Bio inert Materials 11 Overview of theModule 13 Autosampler Principle 15 Early Maintenance Feedback 21 Instrument Layout 22 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Specifications 28 Installing the Autosampler 31 Unpacking the Auto
104. rs 3396 Series Il 3395A integrator see details in section Remote Cables on page 160 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Identifying Cables 12 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection RS 232 cables p n Description G1530 60600 RS 232 cable 2 m RS232 61600 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null M odem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 157 12 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The ot
105. rtment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 13 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Hardware Information 13 Table 14 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 15 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 16 Parity Settings without on board LAN Switches Parity 7 e 0 0 No Parity 0 1 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 181 13 Hardware Informa
106. s not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides WARNING Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 121 10 Maintenance Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety s
107. sampler 32 Optimizing the Stack Configuration 34 Installing the Autosampler 39 Flow Connections to the Autosampler 41 Installation of stainless steel cladded PEEK capillaries 42 LAN Configuration 43 Setting up the module ina LAN environment 44 Connecting the module via LAN 45 Using the Module 47 Preparing the Autosampler 48 Setting up the Autosampler with Agilent ChemStation 52 M ain Screens of the Autosampler with Agilent Instant Pilot G4208A 62 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Contents Optimizing Performance 65 Delay Volume and Extra Column Volume 66 How to Configure the Optimum Delay Volume 67 How to Achieve Higher Injection Volumes 69 How to Achieve High Throughput 71 How to Achieve Higher Resolution 72 How to Achieve Higher Sensitivity 75 How to Achieve Lowest Carry Over 76 Troubleshooting and Diagnostics 79 Overview of the M odule s Indicators and Test Functions 80 Status Indicators 81 User Interfaces 83 Agilent Diagnostic Software 84 Error Information 85 What are ErrorMessages 87 General Error Messages 88 Module Error Messages 94 Test Functions and Calibration 105 Introduction 106 System Pressure Test 107 Sample Transport Self Alignment 109 Maintenance Positions 111 Injector Steps 115 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 10 Maintenance 119 Introduction to Maintenance 120 Warnings and Cautions 121 Overvi
108. screw driver carefully lift out the needle 3 Disconnect the seat capillary from the Injection valve seat from the holder 132 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Next Steps 5 Insert the new Needle seat Press it firmly in position 6 Inthe user interface exit the Change needle seat function and exit the maintenance mode In the Lab Advisor software the Change needle seat function can be found in the Tools section Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 133 10 Maintenance Replacing the Rotor seal When Tools required Parts required Preparations CAUTION Poor injection volume reproducibility Leaking injection valve Description Wrench 1 4 inch Hexagonal key 9 64 inch p n Description 0101 1416 Rotor seal 3 grooves max 600 bar Remove front cover Remove the leak tubing if necessary Damage of the stator face The stator face is held in place by the stator head When removing the stator head the stator face might fall out of the valve gt When removing the stator head ensure the stator face does not fall out of the valve 134 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Replacing the Rotor seal 1 Remove all capillary fittings from the injection valve ports 2 Loosen each fixing bolt two turns at a time Remove the bolts from
109. sensors on the sampling unit flex board The sensors detect the successful completion of the needle lock movement If the needle lock fails to reach the end point or if the sensors fail to recognize the needle lock movement the error message is generated Probable cause 1 Defective or dirty position sensor 2 3 4 Needle to Needle Seat Position Sticking spindle assembly Defective needle drive motor Defective main board Suggested actions Clean the position sensor Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The needle failed to reach the end position in the needle seat The position of the needle is monitored by a position encoder on the needle carrier If the needle fails to reach the end point or if the encoder fails to recognize the needle carrier movement the error message is generated Probable cause 1 Bad sample transport sampling unit alignment Bent needle Missing needle Blocked seat Defective position sensor in the needle carrier assembly Defective 1260 Infinity board Suggested actions Do an auto alignment Check and exchange the needle assembly if necessary Clean or change the needle seat assembly if necessary Exchange the needle carrier assembly Please contact your Agilent service representative Please contact your Agilent service representative Agilent 126
110. ser M anual 89 8 90 Error Information Synchronization Lost Leak During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Leak Sensor Open Error Information 8 The leak sensor in the module has failed open circuit
111. ss the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Error Information 8 Fan Failed The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your A
112. st Functions and Calibration 9 Action Comments Valve Bypass Plunger Home Needle Up Move to Location Needle into Sample Draw Needle Up Needle into Seat Valve Mainpass Needle Up Mainpass Switches injection valve to the bypass position Moves the plunger to the home position Lifts the needle arm to the upper position M ove the needle arm to the vial location on the plate Lowers the needle into the vial Metering device draws the defined injection volume Lifts the needle out of the vial Lowers the needle arm into the seat Switches the injection valve to the mainpass position Moves needle arm to waste position and switches the injection valve to the mainpass position Command also switches the valve to bypass if itis not already in that position Command lifts the needle and lowers the needle into the sample Command can be done more than once maximum draw volume of 40 100 4000 uL cannot be exceeded Use Plunger Home to reset the metering device Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 117 9 Test Functions and Calibration Injector Steps 118 Agilent 1260 Infinity Bio inert High Performance Autosampler User Manual Agilent 1260 Infinity Bio inert High Performance Autosampler User K M anual e ee 10 e Maintenance J Introduction to Maintenance 120 Warnings and Cautions 121 Overview of Maintenance 123 C
113. t are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 87 8 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required 88 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Shut Down Error Information 8 An external instrument has generated a shut down signal on the remote line The module continually monitors the remote input connectors for status signa
114. tandards appropriate for the type of external equipment CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molecules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system 122 The electronics of the autosampler will not allow operation of the autosampler when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the fan immediately Voltages for the other electronic components will be turned off after 30 seconds The status lamp will light up red and an error will be logged into the logbook of the user interface Always operate the autosampler with the top covers in place Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Overview of Maintenance The following pages describe maintenance simple repairs of the autosampler that can be carried out without opening the main cover Table7 Overview of Maintenance Procedure Typical Frequency Notes Change needle needle 60 000 needle into seat seat Change metering seal 30 000 injections Peristaltic pump 3000 hours on time cartridge Change rotor seal 30 000 i
115. tector flow cell Detector parameters In addition the discussion on detector parameters also mentions the related topics of selectivity and linearity Columns Sensitivity is specified as a signal to noise ratio S N and hence the need to maximize peak height and minimize baseline noise Any reduction in peak dispersion will help to maintain peak height and so extra column volume should be minimized by use of short narrow internal diameter connection capillaries and correctly installed fittings Using smaller inner diameter columns should result in higher peak height and is therefore ideal for applications with limited sample amounts If the same sample amount can be injected on a smaller i d column then the dilution due to column diameter will be less and the sensitivity will increase For example decreasing the column i d from 4 6 mm to 2 1 mm results in a theoretical gain in peak height of 4 7 times due to the decreased dilution in the column For a mass spectrometer detector the lower flow rates of narrow columns can result in higher ionization efficiencies and therefore higher sensitivity Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 75 6 Optimizing Performance How to Achieve Lowest Carry Over 76 Carryover is measured when residual peaks from a previous active containing injection appear in a subsequent blank solvent injection There will be carry over between active injections which may
116. ted actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Motor belt tension too high Switch off the module at the power switch Wait at least 10 minutes before switching on again 4 Defective motor Please contact your Agilent service representative 5 Defective transport assembly flex board Please contact your Agilent service representative Invalid Vial Position The vial position defined in the method or sequence does not exist The reflection sensors on the transport assembly flex board are used to check automatically which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause Suggested actions 1 Incorrect tray installed Install the correct trays or edit the method or sequence accordingly 2 Incorrect tray definition Install the correct trays or edit the method or sequence accordingly 3 Incorrect vial positions defined in the Install the correct trays or edit the method or method or sequence sequence accordingly 4 Tray recognition defective dirty sample tray Ensure the coding surfaces of the sample or defective transport assembly flex board tray are clean located at the rear of the sample tray e Please contact your Agilent service representative Agilent 1260 In
117. th limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included 84 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual e ee a o e e co Error Information What are Error Messages 87 General Error Messages 88 Timeout 88 Shut Down 89 Remote Timeout 89 Synchronization Lost 90 Leak 90 Leak Sensor Open 91 Leak Sensor Short 91 Compensation Sensor Open 92 Compensation Sensor Shot 92 Fan Failed 93 Module Error Messages 94 Exhaust Fan Failed 94 Front Door Error 94 Side Door Error 95 Arm M ovement Failed or Arm Movement Timeout 95 Valve to Bypass Failed 96 Valve to Mainpass Failed 96 Needle Lock Failed 97 Needle to Needle Seat Position 97 Needle Carrier Failed 98 Missing Vial or Missing Wash Vial 98 Initialization Failed 99 Metering Home Failed 100 MotorTemperature 100 Invalid Vial Position 101 Peristaltic Pump Error 102 Agilent Technologies 85 8 Error Information Vessel or Wash Vessel Error 102 Vessel Stuck to Needle 103 Rear Blind Seat Missing 103 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 86 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Error Information 8 Wha
118. the head 3 Remove the stator head stator face and stator ring 4 Remove the rotor seal and isolation seal Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 135 10 Maintenance Replacing the Rotor seal 5 Install the new rotor seal and isolation seal Ensure the metal spring inside the isolation seal faces towards the valve body 6 Install the stator ring with the short of the two pins facing towards you at the 12 O Clock position Ensure the ring sits flat on the valve body 136 8 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Replacing the Rotor seal 9 Reconnect the pump capillaries to the valve ports 10 Slide the waste tube into the waste holder in the leak tray a 11 Install the front cover Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 137 10 Maintenance Removing the metering seal When When poor injection volume reproducibility or when metering device is leaking Tools required p n Description 8710 0510 Y4 inch wrench 8710 2392 4mm Hex key Parts required p n Description 1 G5611 21503 Piston seal bio inert 1 Inthe user interface start the maintenance mode and 2 Open the front door select Change metering device functio
119. tion Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 17 Boot Resident Settings without on board LAN Mode Select SW1 SW2 SW3 SW4 SW5 SW6 SW7 SW8 No LAN TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 18 Forced Cold Start Settings without on board LAN Mode Select SW1 SW2 SW3 SW4 SW5 SW6 SW7 SW8 No LAN TEST BOOT 1 1 0 0 1 0 0 1 182 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Hardware Information 13 Electrical Connect
120. tion at the far back in order to prevent seal and or piston damage Change Metering Device Figure 23 Maintenance Positions Change M etering device 114 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Test Functions and Calibration 9 Injector Steps Injector Steps Each movement of the sampling sequence can be done under manual control This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions enabling the specific step to be done When When troubleshooting the module Parts required Description 1 None Preparations None Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 115 9 Test Functions and Calibration Injector Steps 1 Run the Injector steps with the recommended user interface for further information see Online Help of user interface Qsim Lam DWT pi D Atout te B Agilent Lab Advisor Onet Patrewet E Eroe iainty te joroooceoooj Veretes A 1 0S moree eao CRAL Fa RA gt i Nendo res lew Cm S Ea ww u LLD ev Figure 24 Injector Steps Running 116 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Step Commands Table6 Step Commands Step Te
121. tive covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 187 14 Appendix Safety Symbols Table 19 Safety Symbols Symbol Descript
122. tosampler User M anual Error Information 8 Vessel Stuck to Needle The vessel sticks to the needle when the needle moves up Probable cause Suggested actions 1 Closing mat to rigid thick Check that the closing mat is not too thick 2 Bad X or Theta positioning and the needle Exchange the needle carrier assembly sticks into the wall between two holes 3 Defective encoder on the needle carrier Please contact your Agilent service assembly representative Rear Blind Seat Missing Rear blind seat is missing although claimed to exist by main board information occurs during initialization or if the blind seat location has to be used Probable cause Suggested actions 1 Blind seat is missing Install blind seat Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 103 8 Error Information Module Error Messages 104 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual amp ee 9 A Test Functions and Calibration J Introduction 106 System Pressure Test 107 System Pressure Test Evaluation 108 Sample Transport Self Alignment 109 Maintenance Positions 111 Maintenance Positions 111 Change Needle 112 Change Loop Capillary 113 Arm Position 113 Change Needle Carrier 114 Change M etering Device 114 Injector Steps 115 Injector Steps 115 Step Commands 117 This chapter describes the tests for the mod
123. tray 2 e Get the status of the EM F Early Maintenance Feature 3 e Switch injection valve to Mainpass Bypass 4 Instrument actuals Information e Injection volume Sample location A right click into the Active Area will open a menu to Show the Control User Interface special module settings Show the Method User interface same as via menu Instrument Setup G1367E Set Error Method Identify Device Home Arm Reset Sampler Wash Needle Needle Up Valve Mainpass Bypass Same as click on the valve icon e Switch on Tray Illumination Edit Well Plate Types e Wellplate Configuration same as click on the Tray icon 54 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual EN HiP ALS Injection Volume 0 0 uL Sample Location Injection Volume 0 0 uL Sample Location Using the M odule Setting up the Autosampler with Agilent ChemStation Module Status shows Run Ready Error state and Not Ready text or Error text Error Red Not ready yellow Ready green Pre run Post run purple e Run blue Idle green e Offline dark gray e Standby light gray EMF Status shows Run Ready Error state and Not Ready text or Error text Offline gray Ok No Maintenance required green EMF warning Maintenance might be required yellow e EMF warning Maintenance required red Agilent 1260 Infinity Bio inert High Perfo
124. ule Agg Agilent Technologies 105 9 Test Functions and Calibration Introduction 106 All tests are described based on the Agilent Lab Advisor Software B 01 04 Other user interfaces may not provide any test or just a few Interface Comment Available Function Agilent Instrument Utilities Maintenance tests available System Pressure test e Sample transport Self Alignment Agilent Lab Advisor All tests are available e System Pressure test Sample transport Self Alignment Agilent ChemStation No tests available e Pressure Adding of pressure to chromatographic signals possible Pressure ripple Temperature mainboard For details on the use of the interface refer to the interface documentation Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Test Functions and Calibration 9 System Pressure Test The test determines the leak rate of the system between pump outlet valves and a blank nut The blank nut can be positioned at different locations in the system before the flow cell to determine and verify the leak rate of individual modules and components The test allows for setting the pressure at which the test is performed The leak rate of high pressure parts are not always a linear function and therefore it is recommended to perform the test at a pressure that correspond to the normal operating pressure of the system When In case of a suspected leak To verify successful execution of maintenanc
125. utosampler is calculated from the needle pusher position 8 Remove the silicon safety tube from the needle 9 Inthe user interface exit the Change needle seat function and exit the maintenance mode In the Lab Advisor software the Change needle seat function can be found in the Tools section 10 Re install the side door and close the front door 130 Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Maintenance 10 Exchanging the Needle Seat When Tools required Parts required Preparations When seat is visibly damaged blocked or leaks p n Description 8710 0510 Y inch wrench 8710 0510 Y4 inch wrench flat head screwdriver p n Description 1 G5667 87017 Seat assembly bio inert 0 17 mm ID 100 mm In order to avoid leaks close the shutoff valves in the pump or remove tubings from solvent bottles Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be carefull when you remove the needle carrier assembly gt Use the silicon safety tube supplied with every new needle Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 131 10 Maintenance Exchanging the Needle Seat 1 Inthe user interface start the maintenance mode and 2 Open the front door select Change needle seat function In the Agilent Lab Advisor software the Change needle seat function can be found in the Tools section 4 With a Flat head
126. valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Valve in an intermediate position between Turn the WPS main power OFF and ON the By and the Main position 2 Defective injection valve Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Valve to M ainpass Failed The injection valve failed to switch to the mainpass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Valve in an intermediate position between Turn the sampler main power OFF and ON the bypass and mainpass positions 2 Defective injection valve Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual Needle Lock Failed The lock assembly on the sampling unit failed to move successfully Error Information 8 The upper and lower positions of the needle lock are monitored by position
127. wo maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1260 Infinity Bio inert High Performance Autosampler User M anual 21 1 Introduction Instrument Layout 22 The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and the meta

Agilent 1260 Infinity Bio-inert High Performance Autosampler

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