Manuel Technique
Contents
1. RBC RBC GR Probe voltage WBC OFF SET Tension sonde GAIN WBC GB a HGB Red OFF SET 5 HGB pon Black e e N HGB Potentiometer GAIN OFF SET Black VII 2 1 Hemoglobin channel adjustment CAUTI ON Make sure the counting chambers shield is in place and that there is no possible light interference during this adjustment 2 1 1 HGB offset Disconnect the HGB lamp connector at the Pre amplifier Board input 2 blac2. 1100 5 3 an9 2 X NS 5 a 2 ER 8 a S E 79 0 63 a 3181113 6 gt iz 0 25711 3 ls OAM 0 _ o AWO0Z 9 0 __ 5 OAM PIOUSSIUL 5 gt siii 6dr eo 1 60 3 5 z m 7 11 N 9 m AUG pdf 2 de 5 x 11d PIOUSeUL 5 5 m 0 silos gar lt 2 1 y Ide ogy yoeq Bums 3 N 7 Len 589 gt e Em AWOSL 2 8 lan S x Ody PIOUSSIUL gt pJeoq 01600 Soleuy 4 018600 J13 J2 et J3 J9 IIn Ar dwe 0 9 Lld gt 99rv In Ay via iue A0 9 Fld e 69 g rv J ddn 498 0 OND Gld 0 914 e Nee uvm 8ld e S Lld pJeoq JOJON J1 J5 J6 J7 J10 J11 J14 J15 J16 J17 J18
3. N y K lo N S i Z m Q 8 65 2 yd T 8 FEL se lt m Co 16 38 RBC 715 20 B q 5 JIM AUIS 6 16 e 17 16 95 0 Oda 38 33 35 36 s yndul 10 606 VVaste pump Fluldic schematic for CELLY S N lt B1018 31 janvier 2004 Page 40 HYCEL CELLY Field Service Manual SUES TEM Length ant type of tubing used for CELLY SN B1018 11 12 irt xtis Tygon 52410000 A o 420 1 52240172 2 425 5 64x1 8 Silastic 524100560 8 4 42 1 52240174 a
4. e Sampling needle moves down to the bottom of the WBC cuvette 9 Liquid syringes move down to aspirate the appropriate volume from the 1st dilution e Sampling needle moves up and shake the diluent drop e Liquid syringes carry on their movement upwards to aspirate diluent Second aspiration diluent diluent lysing agent lysing agent EV 1 EV 1 0 18 syringe Sampling Sampling needle WBC chamber Liquids syringe needle Cuve GB First dilution aspiration Diluent aspiration HYCEL Page 22 31 janvier 2004 CELLY Field Service Manual SUE OS TEM I V 6 Second external needle rinsing Sink is positioned under the sampling needle Liquid syringes move down to push 1 5 cc of diluent outside the needle to rinse it diluent lysing agent EV 1 Liquids syringe Sampling WBC chamber needle Waste External needle rinsing IV 7 Second dilution and WBC dilution transfer RBC cuvette is positioned under the sampling needle Electrovalve V2 is activated Liquid syringes move up to push 8 ml of diluent Electrovalve V3 is alternatively activated to ensure a proper mixing of the dilution Simultaneously Electrovalve V4 is activated to select WBC transfer only Electrovalve V1 is activated to dispense lyse Electrovalve V5 is
5. Liquids syringe Sampling needle Sample aspiration Diluent aspiration IV 3 External needle rinsing Sink is positioned under the sampling needle Needle moves down Electrovalve V2 is activated Liquid syringes move up to push diluent 1 5 cc Needle moves up and shake the diluent drop diluent lysing agent EV 1 Liquids syringe Sampling VVBC chamber needle Waste External needle rinsing HYCEL Page 21 31 janvier 2004 CELLY Field Service Manual I V 4 First dilution WBC cuvette is positioned under the sampling needle Electrovalve V2 is activated e Liquid syringes move up to pour 7 ml of diluent Electrovalve V3 is alternatively open or closed to push diluent inside or outside the needle to provide effective mixing of the dilution V2 is deactivated Sampling needle moves up to get rid of the diluent drop in the first dilution and then moves up diluent EV 3 alternately open and close lysing agent EV 1 Sampling needle Liquids syringe WBC chamber WBC cuvette First dilution IV 5 Second aspiration
6. 16 H30 28 38 RBC B 29 17 A 28 14 1 eBuu s JIM AUIS 22 21 eDuu s 23 16 14 ndul 106 xr 0 OA 38 33 35 36 Waste pump Fluldic schematic for CELLY S N gt B1018 31 janvier 2004 Page 42 HYCEL CELLY Field Service Manual vrm an TEM Length ant type of tubing used for CELLY SN B1018 A fevv changes vvere made on the tubings on SN B1018 and the follovving instruments The tubing n 13 between V6 and the metering tube is different as it is better to have a small volume to get the proper detection bubble 11 14 1 16 1 8 Tygon 15241000601 A 9 7420 1 52240172 3 120 56400
7. X d L mn 3 08 pie pie 5 SIMMI n d sinne 2 5 SIMMS 2 3 4 IR v FSO FSI FS2 BFO BFI BF2 PAGE 2 JUMPERS POSITIONS JP6 between 2 and 3 factory set JP8 between 1 and 2 factory set JP10 OPEN JP1 JP2 OPEN JP4 Between 1 and 2 factory set JP5 between 2 and 3 JP24 VIDO OPEN SHORT VID1 OPEN VID2 OPEN VID3 SHORT VID4 FSO between 2 and 3 FS1 between 1 and 2 FS2 between 2 and 3 8 0 between 2 and 3 BF1 between 2 and 3 BF2 between 1 and 2 31 janvier 2004 Page 49 HYCEL CELLY Field Service Manual bioeode HYCFI GNOSTICS ROM PCI ISA BIOS 2A59ISEA STANDARD CMOS SETUP AWARD SOFTWARE INC Date mm dd yy Tue Jun 13 2000 Time hh mm ss 12 11 14 HARD DISKS TYPE SIZE CYLS HEAD PRECOMP LANDZ SECTOR MODE Primary Master Auto Primary Slave None Secondary Master None Secondary Slave None Drive A 1 44M 3 5 in Drive B None Base Memory Extended Memory Video EGA VGA Other Memory Halt On All Errors Total Memory Select Ttem PU PD Modify Fl Help Shift F2 Change Color ROM PCI ISA BIOS 2A59ISEA BIOS FEATURES SETUP AWARD SOFTWARE INC Virus Warning Disabled Video BIOS Shadow Enabled CPU Internal Cache Disabled C8000 CBFFF Shadow Disa
8. 7429 uonn p ogy uonnjp JAM lp u 68 6 aBuuAs spinbiq 9 6 9 2 9 y 89 AlBA juenjp Jaquueyo OEM A 9 A PA eJeudsouuje 6 A PA dois gt eqn yes 4 8110 7 5 19 2 JUIS pue OL A BA 9 iss ld umnn cpA 31 janvier 2004 Page 34 HYCEL CELLY Field Service Manual bioeode YCFI RIO TF CU HF N ATA BY iATAANOSTICS I V 17 RBC draining and rinsing RBC cuvette is drained 9 VS is activated Waste pump is activated Diluent from the RBC dilution cuvette is transferred e VS is activated e Air syringe moves up to push air at the back of the orifices HYCEL Page 35 31 janvier 2004 HYCEI P 4 RIO TF CH N ATA a Y 10 uonn o5 sul 3 3NI 02 Bululeip v v 9 A BA LL A PA JIOAJ S 1 92555810 pue LUnnopA Pu1 ul ue OL 9 9
9. Sampling assembly without adjustment HYCEL Page 74 31 janvier 2004 CELLY Field Service Manual Dioeode 4d HYCFI eHN L R E ac r x TE E 5 m l er Turret with adjustment HYCEL Page 75 31 janvier 2004 CELLY Field Service Manual 0 TEM M 003 Turret vvithout adiustment HYCEL Page 76 31 janvier 2004 CELLY Field Service Manual TEM Liquids syringes serial number lt C1122 HYCEL Page 77 31 janvier 2004 h CELLY Field Service Manual biocode 4d HYCFI 180 Moteur quip 9 8 70 59 250 230 cuysw cum lt Liquids syringes serial number gt C1122 HYCEL Page 78 31 janvier 2004 CELLY Field Service Manual HYCEL Page 79 31 janvier 2004 HYCEI P 4 RIO TF HN nin a Y CELLY Field Service Manual J C U 2 U c a duue G H Jequieu OAM 238 J4 4 lt
10. et LOTS Od j l 3dAL AC NYYOS JUMPERS POSITIONS JP20 between 1 and 2 JP21 between land 3 and between 2 and 4 JP28 between 2 and 3 JP24 between 1 and 2 P54C 3 3V JP6 OPEN JP7 OPEN JP27 SHORT JP16 OPEN JP26 OPEN JP5 SHORT CYRIX 6x86 P166 INTEL 133 MHz JP10 between 2 and 3 JP12 between 1 and 2 JP17 between 2 and 3 JP18 between 2 and 3 JP19 OPEN gt Note Be careful with the CPU fan HYCEL Page 46 31 janvier 2004 bioeode HYCFI R I TF EHMN l n RY CELLY Field Service Manual ROM PCI ISA BIOS 2A59GSEO9 STANDARD CMOS SETUP AWARD SOFTWARE INC Date mm dd yy Wed Jun 14 2000 Time hh mm ss 8 45 30 HARD DISKS TYPE SIZE CYLS HEAD PRECOMP LANDZ SECTOR MODE User 1285 2490 1 65535 2489 63 AUTO Primary Master 6 None 0 0 0 0 0 0 Primary Slave Secondary Master Secondary Slave None 0 0 0 None 0 0 Drive A 1 44M 3 5 in Drive B None Floppy 3 Mode Support Base Memory Extended Memory Other Memory Disabled Video Halt On EGA VGA All Errors Total Memory Select Item Fl Help PU PD Modify Shift F2 Change Color ROM PCI ISA BIOS 2A59GSE9 BIOS FEATURES SETUP AWARD SOFTWARE INC Virus Warning Disabled Video BIOS Shadow Enabled CPU Interna
11. i 31 Janvier 2004 Jequieuo Bununos JAH Jequieuo Dununoo 9 8 9 BIqnop 0 0 AlEA peso C 0 V duwung VN 7 H An uolln lp 110 JAM VN eunsseJd 2g 0015 lt yuls snjo s 9 5 7 CELLY Field Service Manual 8 9 p9JOAJOD JOU VN D IDAII9D V N 5 je Buu s spinbr V Ob 3 yes 4 4 A BA S VN 8 3 VN 9 3 VN GA VN VN 7 VN 3 VN LAJ SnJDJS S A DA 9 9 oDuu s inss ld uunnoBA Z 1108 s udsouue Page 36 HYCEL HYCEI P 4 RIO TF CH N ATA a Y CELLY Field Service Manual 0 8 09 890 0 6 4 89902 TI A EA uado v duwung VN spinbi VN ainsseid uinnopA snyps 8 D IDAIIOD JOU VN V VN cb A3 V LL AJ V LA43 VN A4 V 8 3 VN LAA VN 9 3 VN SA
12. p solo A EBA uedo dund VN spinbi VN eunsseud uunnopA snjpJs SEBULIAS 09 0 80 JOU YN D IDAII9D VW VN CL A3 VN bb 3 7 Ob VN 6A3 VN 8 3 V ZA VN 9 3 VN GA VN VA VN 3 VN CAI VN LA SNIDIS S A DA 1 40 39 9 61 ww 010 ogy lp u Bulj dwes 9 v v JAM 0 17 bugunoo 510150 38111 ad 8 A EA LL 6 Jaquieyo Dununoo sun SEE Dununoo y A BA A PA 9 A PA 2941 89 1 219udsou e E JIOAJ9S9J oJnsseJd pue 5 181 0 gt Z dois 4 B gt AUIS eqni 1 215 4 M 4 58 0 8150 31 janvier 2004 Page 28 HYCEL CELLY Field Service Manual biocode YCFI RIO TF CU H N 0i nav i iATAANOSTICS I V 10O First dilution cuvette rinsing 8cc of diluent are poured in each of the WBC and RBC dilution cuve
13. 5 uz 46 14 66 2680 16h46 ARROWS ito move cursor ENTER to validate ESCito quit Fe Species HYCEL Page 17 31 janvier 2004 CELLY Field Service Manual 111 10 2 Printout setup F2 Choose the best printout for the laboratory Laboratoire Mohamed U Casablanca CANON BJ AAA 216 248 250 2 SHH 4 FiH to display selections 14 66 2600 16h46 ARROWS ito move cursor ESC to quit 111 10 3 Date and time F3 Enter time and date 05 1466 2666 14 66 2606 161145 ARROWS to move cursor EMTER to validate ESC ito qui HYCEL Page 18 31 janvier 2004 CELLY Field Service Manual 8080808007 TEM 111 10 4 Other setup F4 Passvvord 2004 e Heading of the printout laboratory name e Language e Mode Veterinary or human Units Service passvvord 2004 Laboratory passvvord labo VVBC threshold for auto rinse VVBC gt 25000 PLT threshold for auto rinse PLT gt 999000 This rinse avoids all contamination betvveen samples PLT bias 1 or 2 counting sequences Logos Correction factors RBC VVBC PLT HGB RDVV PDVV predilution TOF Time of Flight counting time Negative tolerance 0 5 s alarm S5 Positive tolerance 0 7 s alarm S6 e Serial number geo p Sd n mime m E mr aT 1 a L 4 5 z m 1 1 Tru B B 1 4 D n b 14 66 2600 16h46 ARROWS ito move cursor
14. uonsod 71 jeuDeuloJ9 3 7 9 julodjsa 3 T co 5 E 5 3 At Adus A0 9 kd x 9 2 2 g rv gt C eo 5 s e IN Az 5 i gt e vid due A09 14 6 g n O l iz 5 serv Q 5 gt dns EN 1 B SN a sri 3015 5 Es e 5161513 ogwopegBupws 2 5 E 1 1 x m Vid Acl ld 98M 7 9 OLld AvZ Zld A Gd 2 OAM PIouseu gt 5146 n a2 i 07 ve mo 114 eq 50 i 2 m E s 11 5 7 2 0 am S Te 14 AWG rar __ gt CAINS 54 5 91d 11d Ployseiu 5 gt gt 5005 gar F E S Y ogy yoeq 6 2 5 gt Ody 00 E T a 09 206 2 ES ogHpiousem amp 5 p eoq p eoq N Jeyidue oJg pueoq Dojeuy 01800 4 01600 49 22210 lt NS AT132 10 01314255 2 12 ldi ulid L 3 31 janvier 2004 Page 81 HYCEL CELLY Field Service Manual 5895 room TEM X Spare parts list O ring Viton black Pump 30KNDC wolcable 5251001001 UN Pompe NF30 24volis CELLY N S gt D1277 HYCEL Page 82 31 janvier 2004 e CELLY Field Service Manual RIATFCHNAITARGY DIAGNOSTIC
15. J19 J20 22210 gt NS 33 40 o1 jewayos ojdi2ung D N 31 janvier 2004 0 239 9 6 6 JOJOW 13101 sio 5 8 p eqn oBuu s 7 15 101 DUL S 9 8960 83 7817 3 Page 0 Ajddns 000 0 uonisod 6 70 uonisod 88 01 uonisod BUISJU uonisod 0 gt 3 18060 73 HYCEL HYCEI P 4 RIO TF H N ATA a Y CELLY Field Service Manual due G H Jequieuo 8 994 J4 S lp N Joyoejep 60 960117 8 0 eqn 9 spal 1u0JJ JOJOW 19JJn 56011 6 7 6100055 0 0 uollisod Uonisod RUJ2 U uonisod gt 3
16. peso TI p uonnjp A PA uodo g Z A PA VN dund VN spinbr h An uonnilp V eunsseJd 0015 SNIDIS 50 san AUIS CELLY Field Service Manual D IDAIJOD OU VN Duuejeul Y 00659 VN CL 3 buuAs spinbi VN LL 3 j V 0L 3 x V 63 VN 8 3 VN ZA VN 9 3 V SA3 VN TA4 VN ENI A BA VN 2 3 VN LAA SnJDJS S AIDA juenjp eJeudsoune A BA Buui s amssa1d umn pA 9 A BA s 31 janvier 2004 Page 26 HYCEL CELLY Field Service Manual bioeode YCFI RIO TF CU HF N ATA BY iATAANOSTICS I V 9 WBC line rinse e V10 is activated e Waste pump is activated e V is activated Detergent is aspirated through the WBC aspiration line to fill it with liquid before the count starts e VO is activated 9 V5 is activated To purge the line between V6 and the metering tube e V12 is activated to empty the sink HYCEL Page 27 31 janvier 2004 HYCEI P 4 RIO TF CH N ATA a Y CELLY Field Service Manual 0 uonnjos sul 9AJEA 9 9 4 0
17. H An 7 JAM spinbr L 2 9 9 juen 6 9 LL aS p 8 8 9S Ip eJoudsouge RTE Jaquieyo Dununoo OM dois gt gt eqn Buij uu Hels 4 gt 9 junoo Buunp pesoJo s Udy pue lqqnq aw JIOAJ9S9J oJnssoeJd pue uinnoeA 2 49 1 OL 9 Zi AIQPA 5 0058 8 158910 31 janvier 4 Page 32 HYCEL CELLY Field Service Manual TEM IV 14 RBC WBC first orifice cleaning cycle Using the remaining counting vacuum 9 V7 is activated V9 is alternatively activated and disactivated IV 15 WBC chamber draining WBC chamber is emptied first Waste pump is activated e V10 is activated V11 is activated e V12 is activated The waste pump is first activated for a fixed time then the waste detector is activated and the pump finally stops when there is no more liquid detected The pump membranes are used a lot by a mixing of salted water blood and air Think to change them regularly Less than 50 num day change them 1 time yea
18. 0 1 V on air tube empty with potentiometer AJ69 near PT4 From SERVICE menu select Metering Tube Rinse gt When detergent Hemaref IT passes in front of the detector the voltage read must be less than 4 VDC Make sure the D85 green led lights up VIT 1 2 2 Lower detector Connect the of a voltmeter on PT3 Adjust the voltage read at 6 0 VDC 0 1 V on air tube empty with AJ68 near PT3 From SERVICE menu select Metering Tube Rinse When detergent Hemaref IT passes in front of the detector the voltage read must be less than 4 VDC Make sure the D86 yellow led lights up VII 1 3 Turret detectors adjustment All four detectors must be adjusted to 2 5 VDC 0 1 V out of a detection position Connect the of a voltmeter on e PT5 and adjust with potentiometer AJ70 origin detector on the right Red led D90 e PT6 and adjust with potentiometer AJ71 cuvette pos detector on the left Red led D89 e PT7 and adjust with potentiometer AJ72 external pos detector Red led D88 PT8 and adjust with potentiometer AJ73 internal pos detector Red led D87 NOTE D89 and D90 are OFF when the detection is activated D87 and D88 are ON when the detection is activated HYCEL Page 65 31 janvier 2004 CELLY Field Service Manual Nani TEM VII 2 Pre amplifier board All potentiometers are indicated on the pre amplifier cover W ellow
19. 4 1 2 fv Ay 2 ERU i S 0 5 y 3 bs 5 NG 0 ut u Pu r 0 1 27512 20 franc Tea 3 i 2 B 8255 1 288 126935202 HYCEL Page 57 31 janvier 2004 CELLY Field Service Manual bioeode YCFI V1 6 3 Analog controller The Analog Controller makes the analog signals coming from the Pre Amplifier board understandable by a microprocessor Each analog signal is analyzed according to its size and determines the size of a cell There are three converters e One for WBC and HGB a switch is selected by a microprocessor e One for RBC e One for PLT l JP1 46 RBC NPUT c us 2189 6 Dis 3 T TLO amp DE 83190 2 JP3 PLT NPU P2 72 12 4538 DJ m O 8 3 2021 J 7 i 1621 px 21 se 1 a 2 2 LELE 4121 8 i 1 2 z l E 2 i m IN x e o LE A S a 25 m m oe a o 6 EZI T LTM v E T BE m D 2 4H a D Oo r sJ o amp 5 D P m S bEs x 323 mc 807575 18 R28 8
20. SS HOS SNS SIS N Sh SINS SSIS SON Pees 02 16 9109 mu 2 48 1 20808 16h46 ARROWS to move cursor ENTER to validate E amp C to quit Polip amp PyDn other page HYCEL Page 111 12 31 janvier 2004 bioeode HYcr w siw ny ni 4 NDTD KSTI CELLY Field Service Manual 111 7 3 Blood control results F3 e Results F1 e Statistics F2 e Graphs F3 e To display selections F10 Laboratoire Mohamed Z HYCEL Casablanca F1 RESULTS F2 STATISTICS F3 GRAPHS FiH to display selections 14 06 2600 16h46 ARROWS to move cursor EMTER to validate ESC to quit aboratoire Mohamed Casablanca g HYCEL x 2000 BLOOD CONTROL RESULTS ARROWS ito move cursor E amp C to quit Pol PuyDn other page ire Mohamed Casablanca sf HYCEL BLOOD CONTROL RESULTS Fi RESULTS F3 GRAPHS 14 66 2880 16h46 ESC ito quit HYCEL Page 111 13 31 janvier 2004 ni 4 NDTD KSTI CELLY Field Service Manual ESC ito quit Polip FoDn other pages 111 8 Priming F6 e Hematon plus F1 e Cellyse e Hemaref 11 e All reagents Laboratoire Huhamed U Cazahlanca CELLYSE 5 HEMAREF II ALL REAGENTS 14 66 2660 16h46 ARROWS to move cursor EMTER to validate ESC ito quit HYCEL Page III 14 31 janvier 2004 CELLY Field Service Manual I11 9 Service F7 III 9 1 Service F1 Mechanical and fluidic tests are ava
21. 8 011 Da 7T4LS132 JPi 82 5 U19 JPS WBC 023 ka 24 JP6 U THRI 8 PS P6 R23 22 apli 21 E L10 CI ANA 29 1 HYCEL Page 58 31 janvier 2004 h CELLY Field Service Manual Dioeode HYCFI VI 6 4 Motor driver board This board interfaces all the motors valves and sensors POMPE D EVACUATION SERINGUE A AIR SERINGUE DE LIQUIDES TOURELLE AIGUILLE ORIGINE INTERNE CUVES A YAN lt 5 Of 29 u y Az oa 0 o LI lt 2 o lt uz Aq 5 Yo lt o VALVES CONNECT ADAPT y HYCEL Page 59 31 janvier 2004 HYCEI P 4 RIO TF HN ATA RY CELLY Field Service Manual 61 3 lt 3 01 n E EHE 0 I 1dHU Jd T MI AGE bIr D Co Cp m a LR CTI 6037 d30 MIU 5 9A3 lt 3 11 EF 1 9 184 2 Oo 1 6 1632 CINIA 1 190 3011017 95 TA goru lt xu S el pol s Ls 1 02000 Cor 1 and br 3N431X3 5 0 15 57 z 0 T a 7 0 o LJ m NT x C 1 1014 CI 964 31 A T M 0
22. Preamplificationf 0 ref B200271 EG dE SE D HYCEL af ne 2 Bu EIE HYCEL Page 62 31 janvier 2004 CELLY Field Service Manual biocode YCFI VII Electronic adjustments VII 1 Motor driver board MI I 1 34d EIC a i l 01 394 I C TTY PT3 6 0 0 1 V AJ68 AIR Hemaref lt 4V SG AIR LEDS 45 416 PTS 2 5 0 1V AJ73 ES 12097208 27 UG et 22 2 b 290 ES ped PT7 2 5 0 1V 2 SG LIQUIDE J IC 12 8 lt 4 216 2 5 0 1V AJ71 2 PT4 6 0 0 1V 2 69 AIR au 2 Hemaref lt 4V Xe 4 089 m m PT1 6 0 0 1V Fd ji ETT Ja 166 AIR 5 j Hemaref lt 4V ma VIDANGES l IN L uu mos N k J6 09 IC43 AIGUILLE 1 1210 IC45 C89 L bozi REACTIFS vas PTS 2 5 0 1V AJ70 21 Motor driver board Serial number lt 7 HYCEL Page 63 31 janvier 2004 CELLY Field Service Manual SUES we TEM 41 5 a 37
23. et percentage et absolute value e Middle cells et percentage et absolute value e Granulocytes et percentage et absolute value 1 1 3 Graphiques e WBC histogram e RBC histogram e PLT histogramme 1 1 4 Throughput 40 to 45 tests hour depending on configuration 1 1 5 Sample mode Whole blood 32 uL or prediluted 25 uL HYCEL Page 2 31 janvier 2004 CELLY Field Service Manual gt X4Artewunrnne TEM 1 1 6 Storage capacity CELLY can keep in memory more than 100000 results with histograms 1 1 7 Precision GR lt 3 5M GB lt 3 10000 HGB lt 3 14g dL HTC lt 3 45 PLT lt 6 250000 1 1 8 Computer Pentium mother board QVVERTY 84 keystrokes keyboard 9 1 4 LCD color screen Hard disk 2 1 2 floppy disk RS232 output host computer Parallel output optional printer 1 1 9 Sizes and weight Height 42 cm Width 62 5 cm Depth 42 cm Weight 25 kg 1 1 10 Power supply 230 V 10 50Hz 115 V 10 60Hz Povver 300 VA 2 General description 1 2 1 Reagent system Celly uses only 4 different reagents e Hematon plus diluent e Hemaref II detergent e Cellyse lysing agent e Dataclean end of day cleaner 1 2 2 Electronic system There are 9 electronic boards in the CELLY and 3 electronic circuits 1 2 3 Huidic system CELLY uses 4 syringes controlled by 2 24VDC motors step by step motors and also twelve pinch electrovalves HYCEL Page 3 31
24. gt g 666 3 3 o D SU 43 r b 2 r m x w gt e s lt m su o m tx 1k 21 gt A use se cas 6135 Op 4535 95 058 c 5 4 5414 c1 m B l C2 418 258 29 2 U36 27512 L ANH 7 0 R2 29 US1 RS 5 o 829784 35 lu C use Eerd RA 1K C4 l 9 8 9 14 e O 0 82608 6007 01 x RG HYCEL Page 56 31 janvier 2004 h CELLY Field Service Manual bioeode HYCEI ni 4 NDTDSTI VI 6 2 CPU controller This board integrates 3 microprocessors one for WBC and HGB one for RBC and one for PLT Each microprocessor communicates with the PC and controls e The beginning of the count e The probe voltage e The counted values sent to the PC On the WBC and HGB microprocessor when the WBC probe voltage is disabled the HGB measurement is validated M NE _ E ES a Ur gt I n SD 74M6 T1245 2 5 2 5 gs 2 J mi tu ZU 27512 m CIS S 3 8 N F T ub 2 n J 4 27 0 x ag 27512
25. 52 22 Active in Suspend Disabled VGA Active Monitor Enabled soft Off by PWR BTTN Delay 4 Sec Resume by Ring Enabled IRQ 8 Break Suspend Disabled Select Item Fl Help PU PD Modify F5 Old Values Shift F2 Color F6 Load BIOS Defaults F7 Load Setup Defaults ROM PCI ISA BIOS 2A59ISEA PNP PCI CONFIGURATION AWARD SOFTWARE INC PNP OS Installed No Resources Controlled By Manual Reset Configuration Data Disabled PCI IDE IRQ Map To PCI AUTO Primary IDE INT Secondary IDE INT B Used MEM base addr IRQ 3 assigned to Legacy ISA N A IRQ 4 assigned to Legacy ISA IRQ 5 assigned to PCI ISA PnP IRQ 7 assigned to PCI ISA PnP IRQ 9 assigned to PCI ISA PnP IRQ 10 assigned to PCI ISA PnP 150 11 assigned to PCI ISA PnP IRO 12 assigned to PCI ISA PnP IRQ 14 assigned to PCI ISA PnP IRQ 15 assigned to PCI ISA PnP DMA O assigned to PCI ISA PnP Select Item DMA 3 assigned to PCI ISA PnP Fl Help PU PD Modify DMA 5 assigned to PCI ISA PnP F5 Old Values Shift F2 Color DMA 6 assigned to PCI ISA PnP F6 Load BIOS Defaults DMA 7 assigned to PCI ISA PnP F7 Load Setup Defaults HYCEL Page 54 31 janvier 2004 CELLY Field Service Manual bioeode YCFI V1 4 2 Video board and screen All infor
26. 636 Car 12 3032 532 Tygon 1524100070 132 079 sr 30 332532 Tygon 524100070 3327 238 33 55 xt Tygon 1524100100 532 397 34 25 1x14 Tygon 524100100 564 198 5 6 T Tygon 824100100 5 30 18 4 Tygon 1524100100 38 210 16x18 Tygon 524100050 14 116118 Tygon 524100050 Cat 450 18x114 Tygon 1524100100 az 450 Tygon 1524100100 HYCEL Page 43 31 janvier 2004 CELLY Field Service Manual VI 3 Electronic compartment V1 3 1 Electronic boards PC Povver Supply 28V Power Supply Board Pentium Mother Board Video Controller Fluidic Controller CPU Controller Analog Controller Motor Driver Board Pre Amplifier Board V1 3 2 Electronic circuits e Valves Connection Circuit e Draining Detector Circuit e Metering Tube Detectors circuit VI 4 Computer compartment V1 4 1 Mother board CELLY is controlled by a Pentium Mother Board This main board is a high performance system hardware based on Intel Pentium processor It is equipped with 4 master PCI slots 4 sixteen bit ISA slots 2 serial ports 1 parallel port bi direction 1 floppy port The information found in the Setup program is stored in a battery backed CMOS RAM There is different version of the motherboard always equipped with a microprocessor Pentium compatible themselves Moreover 2 Mo are eno
27. ENTER to validate ESC to quit The distributor logo can be change put your logo in a BMP format with shape 184x56 pixels and 16 colors Place it in the directory C HYCEL BMP For changing the instrument logo put your logo in BMP format with shape 240x80 pixels and 16 colors Place it in the directory C HYCEL BMP The software automatically suggests the files in the good format F10 HYCEL Page 19 31 janvier 2004 h CELLY Field Service Manual bioeode HYCFI IV Fluidic cycles description Fluidic cycles are quite difficult to describe as many operations happen simultaneously The comprehension of the cycles is essential for the CELLY maintenance As all hematological counters the leakages and the blocking are the main causes of the dysfunction of the CELLY IV 1 Mode stand by e All syringes pump and valves are inactivated e Counting chambers are empty unless an automatic rinse has been done In such a case they are half full with diluent e Dilution cuvettes and sink are empty open e valve Rinse solution p Vacuum pressure NA Liquids dilution Pum cuvette NA not activated closed e valve double e valve Syringes status RBC ling e valve 7 samp needle e valve 3 Liquids syringe draining d
28. activated to seal the WBC chamber Electrovalve V10 is activated to seal the internal vacuum and pressure reservoirs Air syringe moves down to provide vacuum in the WBC chamber WBC dilution is transferred from WBC cuvette to the WBC counting chamber and lysing agent is added The mixing WBC dilution and lysing agent is done in the T n 27 see pages VI 2 and VI 4 The injection of this mixing in the counting chamber is done tangentially This mixing homogenization is essential for a good numeration a good differentiation and a good measurement of hemoglobin V4 is deactivated HYCEL Page 23 31 janvier 2004 HYCEI 7 2 RIO TF HN ATA a Y CELLY Field Service Manual 23513051 01 NOILN NG 994 e3dsNVe L NOILN NG OAM Jojoejep BurureJp 10 v v dund SEAA oJnssaJd pue uInnoBA 5 19 5 8 8 6 OL 9 uonnjos sul Jequieuo Jaquieyo Dununoo JAM yp A PA 9 e qnop D S0 9 20 uonnjip Ody p us u do VN dung 6 7 7 v eunsseJd uunnopA snjoJs 568 a qn dois gt lt D IDAH D JOU VN Bureau D JDAIIOD V
29. e THE d l m ite D sz np T PT3 6 0 0 1 V 5 55 AJ68 AIR Re Hemaref lt 4V gt 1 77 5 a 4 Ix 214 6 0 0 1 V AJ69 AIR Hemaref lt 4V 12 m7 PTS 2 5 0 1 V AJ73 211 6 0 0 1 V AJ66 AIR Hemaref lt 4V GE VO NE DRE 217 2 5 0 1 V 2 WA LE 37 IC 4 Wd PTS 2 54 0 1 V 70 41 216 2 5 0 1 V AJ71 Motor driver board Serial number D1277 The ground is found on test point GND CAUTION avoid powerful external light interference during these adjustments HYCEL Page 64 31 janvier 2004 CELLY Field Service Manual bioeode YCFI V11 1 1 Draininq detector adiustment Connect the of a voltmeter on PT1 Adjust the voltage read at 6 0 VDC 0 1 V on air tube empty with potentiometer AJ66 near PT1 Pour diluent or Dataclean in the sink on the cuvettes block From SERVICE menu select DRAIN SINK The waste pump will empty the sink When Dataclean passes in front of the VVaste Detector the voltage read must be less than 4 VDC Make sure the D94 red led on the Motor Driver Board lights up V11 1 2 Metering tube detectors adiustment VIT 1 2 1 Upper detector Connect the of a voltmeter on PT4 Adjust the voltage read at 6 0 VDC
30. janvier 2004 CELLY Field Service Manual TEM 1 2 4 Mechanical system Le CELLY mechanical system is composed of tvvo mechanical assemblies controlled by tvvo 24 VDC step by step motors e Sampling needle assay e Turret assay HYCEL Page 4 31 janvier 2004 CELLY Field Service Manual TEM II Theory of operation I1 1 Particle detection method CELLY uses two apertures of defined diameter and length for cell detection and employs the resistance variation principle An internal and an external electrode each at one side of the aperture are connected to a constant current source The cells are suspended in an electrolyte solution of buffered isotonic diluent A vacuum is applied to the back of the aperture in order to pull the cells through the orifice Cells are present a short time in the sensing zone of the orifice and increase the resistance of the aperture displacing an equivalent volume of diluent The constant current source tries to maintain that current with a proportional increase in voltage An amplifier produces a signal which represents the volumetric size of the cell within the sensing zone WBC RBC and PLT have a separate amplification line even if RBC and PLT have a common pre amplification line Each parameter is converted separately 11 2 and RBC PLT theory 11 2 1 WBC The WBC dilution is drawn through a ruby aperture 80 um in diameter and 80 um in len
31. less than 60 mV peak to peak if connected at the back of the analog board or less than 20 mV peak to peak if connected on the GR input point the signal at the back is multiplied by 3 VIT 2 3 2 Platelets offset Connect an oscilloscope on test point JP3 PLT INPUT on the Analog Board and test point GND or a connect a Cinch cable equipped at the other end with an oscilloscope connector on the first connector from the top at the back of the analog controller Adjust the zero offset voltage to 0 00 V with the best precision you can using PLT offset potentiometer located on the pre amplifier board Always check during a diluent count that the offset is properly adjusted and that the background signal is stable and less than 130 mV peak to peak if connected at the back of the analog board or less than 40 50 mV peak to peak if connected on the PLT input point the signal at the back is multiplied by 3 HYCEL Page 67 31 janvier 2004 CELLY Field Service Manual bioeode HYCFI VII 2 3 3 WBC offset Connect an oscilloscope on test point JP5 WBC INPUT on the Analog Board and test point GND or a connect a Cinch cable equipped at the other end with an oscilloscope connector on the third connector from the top at the back of the analog controller Adjust the zero offset voltage to 0 00 V with the best precision you can using WBC offs
32. time is about 8 seconds depending on the WBC orifice and glass metering tube tolerances When this TOF is 1 second above the reference time an alert S6 is displayed When WEC TOF is more than 12 seconds an alert S7 is displayed If WBC TOF is too short 0 5 s under the reference time an alert S5 is displayed HYCEL Page 5 31 janvier 2004 CELLY Field Service Manual bioeode YCFI 11 2 2 RBC and platelet theory RBC and PLT determination is done on a fixed time through a ruby aperture 60 um in diameter and 80 um in length CELLY performs 2 sequences of 8 seconds each Each sequence is divided in 4 partitions of 2 seconds vvhich are compared one vvith another to check if there has not been any trouble clog air bubble during the count The program calculates the mean value of the 4 partitions the Standard Deviation and the Coefficient of Variation The CV tolerated for each sequence according to the number Of particles counted is set in a hidden menu In case the CV calculated is higher than the CV tolerated the program refects the partition vvhich is the furthest from the mean value A nevv CV is then calculated vvith the 3 remaining partitions If this CV is again too high the whole sequence is rejected and it is not taken into account for the final result displayed If the CV is acceptable the sequence composed of onl
33. 0 0 0 2 22 2 222 2 2 222 22 222 02 2 c mm r 255335211212111 255335211212111 amp 00000600000 00 0 0 255213953112111 3 ls mim 88 86 88 86 88 88 8 88 88 mimi mim ls mimlmimimimimimimimimimimimimimimin minim Baba abo Pa F Fa a Fa Fa a I n s a a 14 86 2066 16h46 ESC ito quit There are the same tables for the WBC and the PLT They help to check the blood count on the 256 channels HYCEL Page 16 31 janvier 2004 CELLY Field Service Manual gt 0 ana 111 9 3 Sequences F3 Password 2004 See pages 11 1 and 11 2 aboratoire Mohamed Casablanca SEQUENCES ESC ito quit 111 10 Setup F8 111 10 1 Reference values F1 In veterinary mode choose the specie and enter or change the reference values re Mohamed Casablanca QUE Sag a 6 6 n 14 86 2066 16h46 ARROWS ito move cursor ENTER to validate ESC ito quit e Fi Hidden menu with setup for veterinary mode lysing agent volume time before count threshold min and max Laboratoire Mohamed U Casablanca 7 83 144 al s 46 5 ao s 20 26 09 38 mum Oe Pein mE Se ET ajon and
34. 12 mewe Tygon 524100050 D 4 52240058 re 420 5 64x1 8 566 s24100560 F 5 201 e 25 sesa Silastic 522100560 L 17 51054670 5 64x1 8 Silastic 524100560 M 2 T430 1 152240172 Z 70 5 64x1 8 Silastic 522100560 4 CCLR 3 52240065 14 65 1 16x1 8 Silastic s24100550 Bi 1 CCLR 5 52240065 last 70800808 a CCLR 7 52240065 Table of correspondence HYCEL Page 41 31 janvier 2004 HYCEI AE RI I TF EHMN l ON V CELLY Field Service Manual MOJJASAO sJoquueuo 0 N o eo RA 9 n An OAM SULI e peeu Buljdwes yndul p u Sulldules yndu 76 Buu s 1u nliq moq s47 6 71 12 NG 6 24 3 8 26r 110 VVBC AZ
35. 13 2000 Time hh mm ss 15 13 33 HARD DISKS TYPE SIZE CYLS HEAD PRECOMP LANDZ SECTOR MODE EE a 2 222 ee Primary Master Auto Primary Slave None Secondary Master None Secondary Slave None Drive A 1 44M 3 5 in Drive B None Base Memory 640K Extended Memory 7168K Video EGA VGA Other Memory 384K Halt On Ail But Keyboard M Total Memory 8192K Select Item PU PD Modify Fl Help Shift F2 Change Color ROM PCI ISA BIOS 2A59ISEA BLOS FEATURES SETUP AWARD SOFTWARE INC Virus Warning Disabled Video BIOS Shadow Enabled CPU Internal Cache Disabled C8000 CBFFF Shadow Disabled External Cache Disabled CCOOO CFFFF Shadow Disabled Quick Power On Self Test Disabled DOOO0 D3FFF Shadow Disabled Boot Sequence gt AC SCSI D4000 D7FFF Shadow Disabled Swap Floppy Drive Disabled D8000 DBFFF Shadow Disabled Boot Up Floppy Seek Enabled DCOOO DFFFF Shadow Disabled Boot Up NumLock Status Off Boot Up System Speed High Gate A20 Option Fast Typematic Rate Setting Disabled Typematic Rate Chars Sec 6 Typematic Delay Msec gt 250 Security Option setup PCI VGA Palette Snoop Disabled Select Item Fi Help PU PD Modify F5 Old Values Shift F2 Color F6 Load BIOS Defaults F7 Load Setup Defaults ROM PCI ISA BIOS 2A59ISEA CHIPSET FEATURES SETUP AW
36. 18 Silastic 524100560 C 1 MTL 220 1 52240056 a 4 Tirin Tygon 1524100050 D 4 T murs 58 5 5 5640016 Silastic 524100560 2 MLLR 2 52240058 6 120 564908 Silastic 5241005601 F 5 MTL2904 7 o 5640 6 Silastic 524100560 G 1 742041075 8 85 564016 Silastic 524100560 H 1 1420400 Co 70 ssis sieste 8 a7 9 825 ster 10 165 564608 Silastic 524100860 L 17 8 1 51054670 m ro Sw 5 220096 M 2 4661 52240172 a 1 Simsi szsronsso Bc GOLRCT 2246065 ras 55 116 8 Silastic 524100550 N 4 CCLR 2 52240065 es 08181 sieste s2esoosso R 4 683 152065 L15 85 Tf xfi8 Silastic 524100550 v 2 CCLR 4 52240065 16 160 16x18 Tygon 524100050 Bi r CCLR 5 52240065 564 178 Silastic 524100560 0 1 CCLR 6 52240065 18 190 116818 Tygon 524100050 J 1 ccur 7 52240065 Le na Tygon s2etoooso 20 40 564918 1 524100560 21 6 564 18 Silastic 524100560 21 65 7866 T sieste 220099 23 160 564618 Silastic 524100560 24 210 564618 sit stic 524100560 25 46 2 73 Table of 26 14 16x18 Tygon 524100050 correspondence 27 140 Tygon 524100050 neh mm 28 180 564 18 Silastic 524100560 1 16 156 78 V8 348 30 200 11618 Tygon 524100050 4
37. 460020040 h in 2 m n m ARIS DONOR Por as OS CC OI ARROWS ito move cursor EkCito quit Polly PuyDn othe SEARCH IIT 6 2 Other files F2 e Retrieve F1 e Erase F2 Then these files have the same configuration as current file aboratoire Mohamed U Casablanca OTHER FILES 24415 SAL 23 83 2000 14 14 15 15 04 2408 11 43 16 Fi Retrieve FZ Erase 2 48 14 66 2660 16h46 ARROWS ito move cursor E amp C to quit Pollp amp PuybDbn other pages Home amp End HYCEL Page 111 11 31 janvier 2004 CELLY Field Service Manual gt 0 ana 111 7 Quality F5 111 7 1 Calibration F1 A table with the calibration targets and changeable factors e Run calibration F7 Laboratoire Mohamed U Cazahlanca 48 14 66 2600 16h46 ARROWS to move cursor ENTER to validate ESC to quit Frito calibrate III 7 2 Blood controls F2 e Print F8 e Delete F9 e Other controls F10 e Other pages PgUp et PgDn Laboratoire Mohamed U Casablanca 65 88 2668 FB PRINT F DELETE 18 OTHER BLOOD CONTROL 2 44 14 06 2808 16h46 ARROWS to move cursor ENTER to validate ESC to quit Polip amp PgDn other page ire Mohamed Casablanca BLOOD CONTROLE 2 ELS FE 5 6 15 46 17 86 48 96 32 58 36 40 9 26 TK
38. 94 1131 5 2 163 Sid 69M UL 5 O J 0002 o gold 2 cn 11 S TE 1 11 If 140 0 Sd CO SITY CZ ord u Sr p MEL 1 er 3080171 3805504 1 I NE 31113071 5 8 311211119 Motor driver board serial number lt D1277 31 janvier 2004 Page 60 HYCEL ries GM HYCFI x bioeode R 1 T N I y CELLY Field Service Manual O m a a a 6 tar r eO WW las ciy gt 31 LG 2 uf 35e IM YvOB any ref 412 D25 IMUA 2 dn 3 1 3 301014 a 11 ry N e t 320 31 3 12 245 gt gt A r 32001 e n gd 4 i 1 N M Motor driver boards serial number gt D1277 31 janvier 2004 Page 61 HYCEL CELLY Field Service Manual aia rina un 0 V1 6 5 Pre amplifier board ni 4 NDTDSTI This board polarizes the counting probes and amplifies the pulses from the orifices The hemoqlobin element is also on this board A ADD 235 T 00032 s erin in 1 1 v z O cisi 5 LT 7 PTS 1 RIT ue no 25 5 Esl
39. ARD SOFTWARE INC Auto Configuration Enabled DRAM Timing 60ns DRAM Leadoff Timing 10 6 3 DRAM Read Burst EDO FP x222 x333 DRAM Write Burst Timing x222 Fast EDO Lead Off Enabled Refresh RAS Assertion 4 Clks Fast RAS To CAS Delay gt 3 DRAM Page Idle Timer 2 Clks DRAM Enhanced Paging Enabled Fast MA to RAS Delay 2 Clks SDRAM CAS Lat RAS to CAS 3 3 SDRAM Speculative Read Disabled System BIOS Cacheable Disabled Select Item 8 Bit 1 0 Recovery Time 1 Fl Help PU PD Modify 16 Bit 1 0 Recovery Time 1 F5 Old Values Shift F2 Color Memory Hole At 15M 16M Disabled F6 Load BIOS Defaults PCI 2 1 Compliance Disabled F7 Load Setup Defaults HYCEL Page 53 31 janvier 2004 bioeode HYCFI R I TF EHMN l n RY CELLY Field Service Manual ROM PCI ISA BIOS 2A59ISEA POWER MANAGEMENT SETUP AWARD SOFTWARE INC Power Management Disabled kk Reload Global Timer Events PM Control by APM Yes IRQ 3 7 9 15 NMI Enabled Video Off Method V H SYNC Blank Primary IDE O Enabled Video Off After Standby Primary IDE 1 Disabled MODEM Use IRQ S Secondary IDE O Disabled Doze Mode Disabled Secondary IDE 1 Disabled Standby Mode Disabled Floppy Disk Enabled Suspend Mode Disabled Serial Port Enabled HDD Power Down Disabled Parallel Port Enabled Throttle Duty Cycle 62
40. CELLY Field Service Manual bioeode YCFI RI TFEHN LLDnD i Field Service Manual V 2 40 HYCEL DIAGNOSTICS Factory 4 rue Galvani Parc d activit s Rue de Gerhoui 91745 Massy cedex BP 6 FRANCE 35651 Le Rheu Cedex t l 33 1 64 53 15 15 FRANCE Fax 33 1 64 53 15 20 T l 33 2 99 14 54 14 E Mail Fax 33 2 99 14 54 25 E Mail HYCEL Page 1 31 janvier 2004 CELLY Field Service Manual gt ooo we TEM I Introduction This service manual is intended to provide information on how to adjust calibrate and repair this analyzer I 1 General specifications CELLY is an 18 parameters hematology cell counter safe simple and reliable 1 1 1 Numeration parameters e WBC White blood cells numeration M uL Lymphocytes or Lym K L and 00 Middle Cells or Mid en K L et en 00 Granulocytes ou Grn en K L et en 90 e HGB Hemoqlobin en g dL 9 RBC Red blood cell numeration M L MCV Middle cell volume fl HCT 70 MCH pg RBC M uL MCHC g dL MCHC g dL HGB g dL x100 HCT RDW e PLT Platelets numeration MPV middle platelet volume PCT Plateletocrite PDW 1 1 2 WBC differential WBC differentiation is made in two forms one measured and one calculates Percentages are measured and absolute values are calculated with percentages and WBC total There are 3 WBC populations in two forms e Lymphocytes
41. ELLY Field Service Manual bioeode HYCEI GNOSTICS ROM PCI ISA BIOS 2A59GSE9 POWER MANAGEMENT SETUP AWARD SOFTWARE INC Power Management Disable Power Down amp Resume Events PM Control by APM Yes IRQ3 COM 2 ON Video Off Method V H SYNC Blank IRQ4 COM 1 ON MODEM Use IRQ og IRQ5 LPT 2 OFF 1806 Floppy Disk OFF Doze Mode Disable IRQ7 LPT 1 OFF Standby Mode Disable IRQ8 RTC Alarm OFF Suspend Mode Disable 1809 2 Redir OFF HDD Pover Dovn Disable IRQ1O Reserved OFF IRQ11 Reserved OFF kk Wake Up Events In Doze 6 Standby IRQ12 PS 2 Mouse OFF IRQ3 Vake Up Event ON IRQ13 Coprocessor OFF IRQ4 Wake Up Event ON IRQ14 Hard Disk ON IRQ8 Wake Up Event ON IRQ12 Wake Up Event ON Select Item Reserved OFF Fl Help PU PD Modify Old Values Shift F2 Color Load BIOS Defaults Load Setup Defaults ROM PCI ISA BIOS 2A59GSE9 PNP PCI CONFIGURATION AWARD SOFTWARE INC PCI IRQ Actived By Level PCI IDE IRQ Map To PCI AUTO Primary IDE INT A Secondary IDE INT B Resources Controlled By Manual Reset Configuration Data Disabled IRO 3 assigned to Legacy ISA IRQ 4 assigned to Legacy ISA IRQ 5 assigned to PCI ISA PnP IRQ 7 assigned to PCI ISA PnP IRQ 9 assigned to PCI ISA PnP IRQ 10 assigned to PCI ISA PnP IRQ 11 assigned to PCI ISA P
42. MPV MCV too but less Sometimes the blocking is so low that MPV is the only parameter affected HYCEL Page 68 31 janvier 2004 CELLY Field Service Manual TEM Run human samples to check the PLT histograms shape A low PLT gain adjustment may result in high PLT results as in such case small RBCs may be counted as PLTs On normal human samples the average PLT high threshold is found between 20 and 30 fL 7 2 4 7 WBC gain adjustment Adjust the WBC gain in order to get the lymphocyte peak on normal control blood as target The lymphocytes peak on CBC 3D normal blood is usually around 80 fL Check that the histograms on normal control blood end up close to the 440 445 fl The final adjustment will be done according to the results obtained on at least 10 human samples The histograms must have a good shape and should end up between 330 fL and 360 fL The lymphocytes peak on human samples is very close to 65 fL CAUTION Always re check offset adjustments after final gain adjustments as they may slightly have changed VI1 3 Analog board For the thresholds and swirling back adiustments the ground must be taken on analog controller on the GND test point Any other ground point may result in wrong adjustments VII 3 1 Counting thresholds adjustments Connect the of a voltmeter on test point JP2 RBC Threshold and adjust the voltage to 150 mV 0 5 mV with potentiometer P1 Connect the of
43. S Power ON OFF swith J 1 5335005001 UN RCA Cinch cables 5344001301 UN Red led W O resistor 5382001401 UN Green led W O resistor 588200150 UN 710061 G710061 Needle protection External electrode courte Cabled buzzer 70050 UN Meter tube protection HYCEL Page 83 31 janvier 2004 e CELLY Field Service Manual RIATFCHNAITARGY DIAGNOSTICS Pre amplifier Board Version gt V2 20 V2 20 Pre Amplifier et pot HGB V2 20 Hard disk front side 8280311 UN Keyboard skin cover rEduit 84T cons Vacuum pressure Assy Evac bas SN gt C1178 C750112 Vacu pressure syringe Evac bas SN gt C1178 C770142 CELLY accessories kit 6796820 UN Reagents tubing kit 67906840 UN O Rings kit for CELLY po G2340 Plastic nipples kit 1 230760 UN Small screwdriver J 5716000301 CANON BJ250 printer 8850527 UN 715800150 9 2846 HYCEL Page 84 31 janvier 2004
44. SA PnP IRQ 12 assigned to PCI ISA PnP IRQ 14 assigned to PCI ISA PnP IRQ 15 assigned to PCI ISA PnP DMA O assigned to PCI ISA PnP Select Item DMA 3 assigned to PCI ISA PnP DMA 5 assigned to PCI ISA PnP DMA 6 assigned to PCI ISA PnP DMA 7 assigned to PCI ISA PnP Fl Help PU PD Modify F5 Old Values Shift F2 Color F6 Load BIOS Defaults Load Setup Defaults HYCEL Page 51 31 janvier 2004 CELLY Field Service Manual bioeode HYCFI CELLY serial number gt D1282 Pentium TX4 Vo Chipset 1 J8 L 431Nl d d OLO3NNO 3 AddO 14 PHO ANNOY di 10718 19d LOTS 3 cit 1015 124 L LOIS 124 M t 59 8551 air 420148 4 13490S ker 5559 ozr JUMPERS POSITIONS J4 OPEN J8 between 2 and 3 J6 between 2 and 3 J11 between 2 and 3 J12 between 1 and 2 J13 between 2 and 3 J14 OPEN J15 SHORT 216 between 1 and 2 and between 3 and 4 217 between 1 and 2 between 3 and 4 between 5 and 6 and between 7 and 8 HYCEL Page 52 31 janvier 2004 bioeode HYCFI R I TF EHMN l n RY CELLY Field Service Manual ROM PCI ISA BIOS 2A59ISEA STANDARD CMOS SETUP AWARD SOFTWARE INC Date mm dd yy Tue Jun
45. VN 73 VN LL 3 V OL 3 VN 73 VN 8 3 VN ZA3 VN 9 3 V GAJI V V VN 073 110 NO Z A PA V CAM 9 ShJDJS S AIDA oJeudsoune Budtues WEIS 4 4 33 G 9 9 7 8 MUS 31 janvier 2004 Page 24 HYCEL CELLY Field Service Manual biocode YCFI RIO TF CU H N 0i nav i iATAANOSTICS I V 8 RBC dilution transfer V5 is activated to seal the counting chamber Electrovalve V9 is activated to avoid that WBC dilution may be aspirated from the WBC chamber towards the RBC chamber V10 is activated Waste pump is activated for a very short time to balance vacuum between internal vacuum reservoir and RBC counting chamber Air syringe pull to aspirate RBC dilution in the counting chamber HYCEL Page 25 31 janvier 2004 HYCFI P 4 RIO TF CH N ATA a Y 14908 8 NOILN WG Oda 40159 9 61 0 v v JIOAJ93 9J k oJnssoJd pue duund 0 0 2 9 8 8 LL 8 6 9 6 9 01 2 uonnios 0 89 Joquieuo 60149 18900890 ogy aS 9 8 9 919800 28M b y 9 9
46. VN VA4 VN A4 VN VN LA SnJDJS S A DA 1 H An uonnjp Ody 60068 40 23919 BUIUIEIP v v OM 0 Joquueuo Dununoo UONNIIP 5 sp nbr1 Z 6 SAJEA 8 499 5 3 bununoo 0 y 8 4 dois 4 lt qn 0 9 9 11215 88 R eJeudsouuje JIOAJ S 1 91 99941 pue 8 OL aajea a 72 AUIS 8065 9 oJnssoJd 31 janvier 4 Page 37 HYCEL CELLY Field Service Manual biveode YCFI iATAANOSTICS V Alarms list Sytemaets Backflush during count Fr quence de comptage Taille 400 HYCEL Page 38 31 janvier 2004 CELLY Field Service Manual urn TEM VI System description VI 1 Fluidic assemblies RBC PLT chamber WBC HGB chamber WBC metering tube Sampling syringe Dil
47. a voltmeter on test point JP4 PLT Threshold and adjust the voltage to 100 mV 0 5 mV with potentiometer P3 Connect the of a voltmeter on test point JP6 WBC Threshold and adjust the voltage to 200 mV 0 5 mV with potentiometer P5 VII 3 2 Swirling back circuits adjustments Note These adjustments must be done with an oscilloscope 1 VDC 10us It is advisable to perform these adjustments during blood counts e g normal control blood as it is easier to visualize the signal in such conditions Connect an oscilloscope on test point JP8 and adjust the pulse length to 50 us with potentiometer P2 for RBC channel Connect an oscilloscope on test point JP9 and adjust the pulse length to 44 us with potentiometer P4 for PLT channel Connect an oscilloscope on test point JP10 and adjust the pulse length to 50 us with potentiometer P6 for WBC channel HYCEL Page 69 31 janvier 2004 CELLY Field Service Manual zs e ex 15 Ua an 43199 el CR3100 21 u3 07575 JP13 19 2 03 ene Denn HYCEL ax no EL INPUT cas 07 55 JPS 1 Tuer amp Dr 83100 3 PLT NPU 46 1 RBC NPUT RESHOLD NP G P2 THRESHOL E Rid JP4 mE Die 011 CI mn gt o wo Ks r D C26 U DA u L19 CI ANA 20 Analog
48. bled External Cache Disabled CCO00 CFFFF Shadow Disabled Quick Power On Self Test Disabled DOOOO D3FFF Shadow Disabled Boot Sequence Ay Cy SCSI D4000 D7FFF Shadow Disabled Swap Floppy Drive Disabled D8000 DBFFF Shadow Disabled Boot Up Floppy Seek Enabled DCO00 DFFFF Shadow Disabled Boot Up NumLock Status Off Boot Up System Speed High Gate A20 Option Fast Typematic Rate Setting Disabled Typematic Rate Chars Sec 6 Typematic Delay Msec 250 Security Option Setup PCI VGA Palette Snoop Disabled Select Item PU PD Modify Old Values Shift F2 Load BIOS Defaults Load Setup Defaults Fl Help ROM PCI ISA BIOS 2A59ISEA CHIPSET FEATURES SETUP AWARD SOFTWARE INC Auto Configuration Enabled DRAM Timing 60ns DRAM Leadoff Timing 10 6 23 DRAM Read Burst EDO FP x222 x333 DRAM Write Burst Timing x222 Fast EDO Lead Off Enabled Refresh RAS Assertion 4 Clks Fast RAS To CAS Delay 3 DRAM Page Idle Timer 2 Clks DRAM Enhanced Paging Enabled Fast MA to RAS Delay 2 Clks SDRAM CAS Lat RAS to CAS 3 3 SDRAM Speculative Read Disabled System BIOS Cacheable Disabled Select Item 8 Bit I O Recovery Time 1 Fl Help PU PD Modify 16 Bit 1 0 Recovery Time 1 F5 Old Values Shift F2 Color Memory Hole At 15M 16M Disabled F6 Load BIOS Defaults PCI 2 1 Compliance Disabled F7 Load Setup Defaults HYCEL Page 50 31 ja
49. board Page 70 nat3505 RIO TF C N ATA av TTT P2 150 0 5 mV P1 PS 2 50 Hs P4 75 0 5 mV P9 4 44us P9 4 44us P6 05 200 0 5 mV 31 janvier 2004 CELLY Field Service Manual biocode YCFI VIII Mechanical adjustments VI11 1 Turret adjustment VIII 1 1 Electromagnet adjustment Select SERVICE menu to be able to move the turret as needed Check visually the position of the electromagnet in the turret locking hole Unlock and lock the electromagnet several times to check if it is perfectly aligned with the turret locking hole If it is not unscrew the two bottom screws to reposition the electromagnet with regard to the turret When the electromagnet is correctly aligned unlock it and check that the turret can move in both directions without friction If the electromagnet touches the turret when it rotates it is possible to move it backwards Unscrew the two little screws maintaining the electromagnet horizontally Find the right position and screw back the two little screws Check again the turret rotation VIII 1 2 Turret assay notched bell adjustment The motor driving belt may need to be adlusted if it is too loose or too tight In this case untighten the screvvs fixing the motor to i
50. bubble The count starts when the bubble passes in front of the upper metering tube detector and stops when it passes in front of the bottom detector Vacuum is about 4 8 Hg at the beginning of the count The vacuum loss at the end of the first sequence is about 0 15 Hg The air syringe moves down after the first sequence to compensate the vacuum loss If the TOF time of flight is too short a rinsing of the WBC line is performed If the TOF is too long a backflush is done and a message SO is displayed Note The vacuum reserve is constituted by the vacuum bottle and the air syringe The syringe motor is switch on to avoid all movement of the piston But it can generate an overheating which give a bad vacuum HYCEL Page 31 31 janvier 2004 HYCEI P 4 RIO TF CH N ATA a Y CELLY Field Service Manual lSBAA duind 9jSeAA A BA uonnjos sul A EA 0 8502 A BA 0 VN dund VN spinbi VN eunsseJd uunnopA snjoJs SOBULIAS D IDAIOD JOU VN D LDAH D V V k 3 VN A3 VN L 3 V 6A4 VN 9A4 VN LAW VN 9 3 VN 2723 VN VN A3 VN VN LAI 0 S A DA uonnip Ody lp u 0068 00 8 ONILN 1OO 10 29 p 680 v v 9 Joqueyo Dununoo odd
51. cal alerts it may mean that the needle driving belt is not correctly adjusted If an adjustment is needed unscrew the bottom pinion screw located at the back of the needle assay and re position the pinion vertically to tighten or slack the belt NOTE the belt must never be too tight as it may prevent the smooth movement of the needle VI11 3 Liquid and air syringes adjustment The lever switch detecting the syringes position must be adjusted in such a way that it is activated about 2 mm before the syringes reach their upper mechanical stop VIII 4 Syringes dismounting Necessary equipment e A little plat screwdriver e A damp smooth close e Some silicon grease gt Note The middle syringe contain lysing agent be careful it can be dangerous Unscrew the metalic plate it maintains the 3 syringes and the white component Pull off the 3 syringes and remove the little rings which maintain the pistons Only a film of silicon grease must be put on the syringes if not a lot of electric noise appear on platelets Check that the syringes are align before wind up So make several counting on diluent to have a result near zero for platlets HYCEL Page 72 31 janvier 2004 CELLY Field Service Manual VI11 5 Mechanical diagrams b X i Sampling assembly with adjustment 0 HYCEL Page 73 31 janvier 2004 CELLY Field Service Manual Yy
52. et potentiometer located on the pre amplifier board Always check during a diluent count that the offset is properly adjusted and that the background signal is stable and less than 50 60 mV peak to peak if connected at the back of the analog board or less than 20 mV peak to peak if connected on the WBC input point the signal at the back is multiplied by 3 VII 2 4 Analog gains adjustment For the gains adjustments all calibration factors must be set to 1 see Calibration menu A CBC 3D control blood must be used Check its expiration date and make sure it was kept in good conditions and properly mixed When gain is increase histogram drift to the right so MCV increases too n ini Time VII 2 3 1 RBC gain adjustment Adjust the RBC gain in order to get the target MCV indicated for the normal blood on the Control Assay Sheet e g CBC 3D On human sample RBC indices should be within normal ranges VIT 2 3 2 PLT gain adjustment It advisable to use a high level control blood to perform this adjustment as the MPV result is more accurate if more particles are counted Never use a low level blood to perform this adjustment Adjust the PLT gain in order to get the target MPV indicated for the high control blood on the Control Assay Sheet e g CBC 3D H The usual mobile PLT high threshold is close to 20 fL Sometimes it s difficult to see it On human blood MPV is 9 fL A partial blocking of the orifice increase the
53. etector dilution cuvette e valve 2 WBC RBC counting chamber e valve 8 e valve 1 e valve 4 STAND BY MODE o o gt 1 o e valve 11 atmosphere ing chamber e valve 6 WBC countin e valve 9 metering tube internal vacuum and pressure reservoir e valve 12 e valve 10 vacuum pressure HYCEL Page 20 31 janvier 2004 CELLY Field Service Manual n TEM IV 2 Sample aspiration In case counting chambers are full with diluent e g after an automatic rinse they are drained first 9 Blood tube is positioned under the sampling needle and turret is locked Sampling needle moves down Liquid syringes move down to aspirate sample red and green leds flicker Needle moves up The sample is return to the user When the red led is on the turret is locked Red led lights up during all dilution counting and rinsing cycle Liquid syringes continue their movement down to aspirate the appropriate volume of diluent diluent diluent EV 1 WBC chamber Liquids syringe EV 3 EV 3 lysing agent lysing agent EV 1 Sampling needle WBC chamber
54. fied before counting in the work list menu with specie name and identification number In the two cases e Delete identification F5 e Print the work list F8 e Insert identification F6 e Delete work list F9 In the right column there is an indication if the sample is run or not e Sample already run e Sample not run 14 06 2000 16 48 ARROWS ito move cursor ENTER to validate ESC to quit III 5 Run F3 e Control patient F1 e Half dilution F7 e Specie veterinary mode F2 e Print F8 e With without work list F3 e Veterinary human mode F9 e Rinse F4 e Platelet threshold F10 e Recount F5 e Main menu ESC e Predilute F6 30 fL 14 66 2066 16h46 BACKGROUND CHECK HYCEL Page 10 31 janvier 2004 1 h CELLY Field Service Manual biveode A HYCEI I11 6 Datalog 4 III 6 1 Current file F1 e GB 08 F1 e See histograms F6 e Search F2 e Transfer RS232 F7 e Next Alt F2 e Configuration RS232 A t F7 e Print current numeration F3 9 Print stats F8 e Daily printout F4 See stats 8 9 Print sequence F5 e Reject acceptation F9 e Save diskette sequence Alt F5 ire Mohamed Cazablanca REJECT 88 Display m Robot baba 0002 PPP PENA pu AA As CO aes mad AL ANA ED m NO NENGA Ja eee a d 3 une af Ie T AD 00000
55. gth CELLY WBC counting time is determined through a 176 uL glass metering tube between two optical detectors CELLY performs 2 counting sequences of about 8 seconds each Each sequence is divided in 4 partitions of 2 seconds which are compared one with another to check if there has not been any trouble during the count clog air bubble The program calculates the mean value of the 4 partitions the Standard Deviation and the Coefficient of Variation The CV tolerated for each sequence according to the number of particles counted is set in a hidden menu In case the CV calculated is higher than the CV tolerated the program rejects the partition which is the furthest from the mean value A new CV is then calculated with the 3 remaining partitions If this CV is again too high the whole sequence is rejected and it is not taken into account for the final result displayed If the CV is acceptable the sequence composed of only 3 partitions will be used in the final result calculation It may happen that both sequences are rejected In such a case a result is calculated but an alert S3 is displayed meaning that the result is not valid The sample should thus be re run The count starts when the rear of a calibrated bubble generated a few seconds before the count starts passes in front of an optical detector And the count stops when the rear of the same bubble passes in front of a second detector The usual TOF Time Of Flight counting
56. high precision it fluctuates during the day When adjustment is over press lt ESC gt to quit and go back to Service menu V11 2 2 RBC and VVBC probe voltage adjustment Connect an electrical adaptor at the input of the pre amplifying board in place of the RBC probe upper connector of the board Connect a voltmeter on this adaptor and select SERVICE menu and then SETTINGS Select Probe Voltages ON Adjust the RBC probe voltage at 20 0 VDC 0 1 V with the potentiometer indicated on the Pre amplifying board cover Then re connect the RBC probe cable Connect the voltmeter together vvith the adaptor to the VVBC connector on the Pre amplifying board and adjust the VVBC probe voltage the same way V11 2 3 Offset adiustment on RBC VVBC channels For the following adiustment the ground must be taken on the analog controller on the GND test point Any other ground point may result in wrong adjustment VII 2 3 1 RBC offset Connect an oscilloscope on test point JP1 GR INPUT on the Analog Board and test point GND or a connect a Cinch cable equipped at the other end with an oscilloscope connector on the second connector from the top at the back of the analog controller Adjust the zero offset voltage to 0 00 V with the best precision you can using RBC offset potentiometer located on the pre amplifier board Always check during a diluent count that the offset is properly adjusted and that the background signal is stable and
57. ilable Test mechanical detectors fluidic detectors et valves Apertures purge before dismounting Drain counting chambers vac pressure reservoir et sink Transfer dilution from cuvettes Rinse metering tube Clean apertures Test metering tube 283888 16h46 ARROWS ito move cursor ENTER to validate ESC to qui e Draining pump On Off e Air syringe vacuum pressure pull push e Liquid syringes pull push Cuvette in out sink WBC RBC and drop e Turret lock unlock and dismounting e Sampling needle down up and drop S7 F VCEL MECHANICS DRAINING PUMP ON AIR SYRINGE PULL LIQUID SYRINGES DOUN CUVETTE BLOCK IH OUT bis TURRET WS ine SAMPLING NEEDLE DOWN UP TEST MECHANICAL DETECTORS FLUIDIC DETECTORS APERTU PURGE BEFORE DISMOUNT ING TURRET EXTERNAL POS CHOETTE NDER NEEDLE SEF HYCEL Page III 15 31 janvier 2004 CELLY Field Service Manual gt 0 ana III 9 2 Settings F2 Password 2004 e Aperture voltages ON F1 e Aperture voltages OFF F2 9 RBC results F3 e WBC results F4 9 PLT results F5 e HGB reference channel F6 e Instrument vvorn out F7 e Continuous rinsinc F9 ire Mohamed Casablanca APERTURE VOLTAGES OM 14 66 2080 16h46 ARROWS ito move cursor ENTER to validate ESC ito quit ire Mohamed Cazablanca HEC RESULTE 3 0 0 0
58. itude increased of about 5 and the two peaks are linked in only one Coincidence is when two cells are in the same time in the orifice To avoid this the CELLY use a statistic treatment of the results 11 4 3 Pulse edit This program is done to process impulsions even if the cells vvere not go in the middle of the orifice HYCEL Page 8 31 janvier 2004 CELLY Field Service Manual TEM III Software description 111 1 Introduction The software application has been developed for Microsoft DOS and IBM compatible computers Menu items are selected with the arrow key and activated by pressing the enter keystroke Menu items are also selected and activated by pressing the given keystroke 111 2 Main menu e Startup Shutdown F1 e VVorklist F2 e Run F3 e Datalog F4 e Quality F5 e Priming F6 e Service F7 e Setup F8 D T LOG SERVICE _ 14 06 2000 68 ARROWS ito move cursor ENTER to validate I11 3 Startup Shutdown F1 e Startup F1 e Shutdown F2 Laboratoire Mohamed U Casablanca SHUTDOUN 14 06 2000 16 48 ARROWS to move cursor EMTER to validate ESC to quit HYCEL Page 9 31 janvier 2004 CELLY Field Service Manual 8080808007 ai TEM I11 4 VVork list F2 If CELLY is in human mode samples are identified before counting in the work list menu with last name and or first name and identification number If CELLY is in veterinary mode samples are identi
59. k and red twisted wires connector Connect a special adaptor at the HGB output of the Pre amplifier Board black Cinch cable Connect a voltmeter according to the polarity and adjust the offset voltage just upper 0 0 mV with the potentiometer indicated on the Pre Amplifier Board cover Reconnect the HGB lamp connector VIL2 1 2 Gain HGB NOTE Wait 20 minutes after put the CELLY on to do this adjustment Like for the usual utilization its better to wait 20 minutes before running startup From Main menu press F7 to Service then F2 Settings enter password 2004 then press F6 for HGB channel setting If necessary the instrument will fill the counting chambers with diluent The HGB reference value is displayed Adjust the HGB gain potentiometer located on the top part of the hydraulic assay near the top of the metering tube to read a channel between 240 and 245 for an ambient temperature around 19 C and 21 C In adjusting between 220 225 the variation can be the best HYCEL Page 66 31 janvier 2004 CELLY Field Service Manual bioeode YCFI This adiustment depends on the temperature HGB reference decreases of about one channel when the temperature increases of one C In any case the HGB reference value is accepted during the startup procedure if it is within 180 and 254 This adjustment does not require a
60. l Cache Disabled C8000 CBFFF Shadow Disabled External Cache Disabled CCO00 CFFFF Shadow Disabled Quick Power On Self Test Disabled DO000 D3FFF Shadow Disabled Boot Sequence A C D4000 D7FFF Shadow Disabled Swap Floppy Drive Disabled D8000 DBFFF Shadow Disabled Boot Up Floppy Seek Enabled DCOO0 DFFFF Shadow Disabled Boot Up NumLock Status Off Boot Up System Speed High Gate A20 Option Fast Typematic Rate Setting Disabled Typematic Rate Chars Sec 6 Typematic Delay Msec 250 Security Option setup PCI VGA Palette Snoop Disabled Select Item F1 Help PU PD Modify Old Values Shift F2 Load BIOS Defaults Load Setup Defaults ROM PCI ISA BIOS 2A59GSE9 CHIPSET FEATURES SETUP AWARD SOFTWARE INC Auto Configuration Enabled Delayed Transaction Enabled DRAM Timing DRAM DRAM Fast DRAM DRAM Fast Fast EDO Path Select Refresh RAS Assertion ISA Bus Clock RAS Precharge Time R W Leadoff Timing RAS To CAS Delay Read Burst EDO FP Write Burst Timing BIOS Cacheable BIOS Cacheable Item Video Select 16 Bit 1 0 Recovery Time Memory Hole At 15M 16M Peer Concurrency Passive Release System HYCEL MA to RAS Delay CLK 70ns 3 x333 x444 x333 1 Disabled 5 Clks PCICLK 4 Disabled Disabled 1 F1 Disabled F5 Enabled F6 Enabled F7 Page 47 Help PU PD Old Values Shift F2 Load BIOS Defaults Load Setup Defaults 31 janvier 2004 Modify Color C
61. latelet Threshold PIt RBC Threshold noise Plt Pulses There are two ways to avoid the swirling back of the cells e Aspiration point just behind the aperture to avoid that the cells cut again the electric field of the aperture This aspiration is done by the electrode which is hollow and long SO it can arrive just behind the orifice The swirling back of the RBC is the most important because it creates a wrong impulsion like a platelet For the WBC the electrode is not long because there is practically no swirling back e The electronic time of passage if a cell re cuts the electric field it creates a new impulsion which has a electronic time of passage more long than normal times This function eliminates the pulses too much distorted due to a new passage of the cell in the orifice electric field For that the time of the pulse is compared to the period of an electric component monostable which can be modified HYCEL Page 7 31 janvier 2004 h CELLY Field Service Manual Dioeode HYCFI 11 4 2 Colncidence Cells with vertical coincidence generatea second population artefact with doubled volume Red blood cells distribution histograms Pulses doubled surface Cells with horizontal coincidence generate an assymetry of the distribution and a loss of cells Red blood cells distribution histograms Pulses ampl
62. mation from the CELLY is visualized on a slim VGA color monitor This screen may be orientated for a better reading comfort For technical maintenance purposes it can also be withdrawn from the computer compartment and set aside from the CELLY CAUTION The Slimage monitor must not be exposed to impact or shock as it is made of fragile glass panel The display area must never be touched or rubbed on with sharp or stiff objects as the glass panel may be scratched Never use solvents to clean it Never let liquid penetrate the monitor Clean vvith a damp smooth cloth Don t expose it at a frozen temperature because the liquid crystals turn into ice and the screen will not be use VI 5 Povver supply compartment There are tvvo independent Povver Supply assemblies in the CELLY V1 5 1 PC power supply The PC Power Supply Assay is 230VV It delivers e 12 VDC 0 5 A e 12 VDC 9A e 5 VDC 23A e 5 VDC 0 3A The 12 VDC VVaste Pump is supplied by the PC Power Assay serial number lt D1277 V1 5 2 28 V and 100 W power supply serial number lt D1277 This board provides 28 VDC to power the valves and the electro magnet It also provides 28 VDC to the Motor Interface Board vvhich is used through a 24 VDC 2A regulator to provide 24 VDC to the stepping motors It also supplies the rear fan with 12 VDC obtained through a regulator fixed on one side of the Power Supply Board metallic case NOTE There are no adjus
63. nP IRQ 12 assigned to PCI ISA PnP IRQ 14 assigned to PCI ISA PnP IRQ 15 assigned to PCI ISA PnP DMA assigned to PCI ISA PnP DMA 1 assigned to PCI ISA PnP Select Item Used MEM base addr DMA 5 assigned to PCI ISA PnP Fl Help PU PD Modify DMA 6 assigned to PCI ISA PnP F5 Old Values Shift F2 Color DMA 7 assigned to PCI ISA PnP F6 Load BIOS Defaults F7 Load Setup Defaults ROM PCI ISA BIOS 2282596529 INTEGRATED PERIPHERALS AWARD SOFTWARE INC IDE HDD Block Mode Enabled IDE Primary Master PIO Auto IDE Primary Slave PIO Auto IDE Secondary Master PIO Auto IDE Secondary Slave PIO Auto On Chip Primary PCI IDE Enabled On Chip Secondary PCI IDE Enabled PCI Slot IDE 2nd Channel Enabled USB Controller Disabled Onboard FDC Controller Enabled Onboard UART 1 Auto UART 1 operation mode Standard Onboard UART 2 Auto Select Item Fl Help PU PD Modify Onboard Parallel Port 378 IRQ7 Old Values Shift F2 Parallel Port Mode Normal Load BIOS Defaults Load Setup Defaults HYCEL Page 48 31 janvier 2004 bioeode HYCFI R I TF EHMN l n RY CELLY Field Service Manual CELLY B1028 lt Serial number lt D1282 Pentium VX3 COMA COMB FLOPPY Jl JP1 JP2 r IOC 19888 8 988 8 8 ISAI ISA3 ISA4 85989888888888888 Z 5
64. nvier 2004 CELLY Field Service Manual bioeode HYCFI GNOSTICS ROM PCI ISA BIOS 2A59ISEA POWER MANAGEMENT SETUP AWARD SOFTWARE INC Power Management Disabled Reload Global Timer Events PM Control by APM Yes IRQ 3 7 9 15 NMI Enabled Video Off Method V H SYNC Blank Primary IDE 0 Enabled Video Off After Standby Primary IDE 1 Disabled MODEM Use IRQ tou Secondary IDE O Disabled Doze Mode Disabled Secondary IDE 1 Disabled Standby Mode Disabled Floppy Disk Enabled Suspend Mode Disabled Serial Port Enabled HDD Power Down Disabled Parallel Port Enabled Throttle Duty Cycle 62 55 ZZ Active in Suspend Disabled VGA Active Monitor Enabled Soft Off by PWR BTTN Delay 4 Sec Resume by Ring Enabled IRQ 8 Break Suspend Disabled Select Item PU PD Old Values Shift F2 Load BIOS Defaults Load Setup Defaults Modify Color Fl Help ROM PCI ISA BIOS 2A59ISEA PNP PCI CONFIGURATION AWARD SOFTWARE INC PCI IDE IRQ Map To PCI AUTO Primary IDE INT A Secondary IDE INT PNP OS Installed No Resources Controlled By Manual Reset Configuration Data Disabled IRQ 3 assigned to Legacy ISA Used MEM base addr IRQ 4 assigned to Legacy ISA IRQ 5 assigned to PCI ISA PnP IRQ 7 assigned to PCI ISA PnP IRQ 9 assigned to PCI ISA PnP IRQ 10 assigned to PCI ISA PnP IRQ 11 assigned to PCI I
65. rs More than 50 num day change them 2 times year I V 16 Final WBC rinsing and backflush Diluent in the WBC dilution cuvette is transferred to the counting chamber to rinse it V5 is activated to lt seal gt the counting chamber e V10 is activated e The air syringe is pulled down to build vacuum in the WBC chamber e V4 is activated to aspirate diluent from the dilution cuvette Simultaneously The back of the RBC orifice is rinsed e V10 is activated e Waste pump is activated V9 is activated 9 V is activated VVBC cuvette is drained V5 is activated to allow air in the air syringe V11 is activated V4 is deactivated Waste pump is activated V10 is activated Backflush is performed on both orifices V 1 is deactivated 9 V12 is activated 9 V9 is activated e Air syringe moves up to push air at the back of the orifices HYCEL Page 33 31 janvier 2004 HYCEI P 4 RIO TF CH N ATA a Y CELLY Field Service Manual 0 duund 0 uonnjos sul qnop peso L 9 VN duwung VN sp nb v eunsseJd 7 1 snjpJs 0 8 D IDAIJOD JOU VN peJoAJoD Y VN A4 VN 3 VN LAI SNIDIS 1 HSN 09089 JO 9 p 6017 v v Q LL A PA Dununoo Og
66. tment on the two Power Supply Boards PC and 28 V The Power Supplies do not have any directly accessible fuse In the event of failure they are on no account to be manipulated V1 5 3 24V and 150 VV power supply serial number gt D1277 This board provides 24 VDC to power the valves and the electro magnet It also supplies the rear fan with 12 VDC obtained through a regulator fixed on one side of the Power Supply Board metallic case The 24 VDC Waste Pump is supplied by this power supply serial number gt D1277 Connect the of a voltmeter on test point JP2 on the motor driver board and adjust the voltage at 24 3 24 4 with the potentiometer located on the power supply just under the red led During the counting cycle the voltage lovver a little HYCEL Page 55 31 janvier 2004 h CELLY Field Service Manual bioeode HYCEI ni 4 NDTDSTI VI 6 Analog boards description VI 6 1 Fluidic controller The Fluidic Controller integrates two microprocessors one for the dilutor V1 V2 and V3 and one for the other valves and the air syringe The Dil microprocessor communicates with the Flui microprocessor and with the PC The Flui microprocessor can t communicate directly with the PC but only through the Dil microprocessor er F 7 r 7 r B uy 3 5 8 3 Iu Bu r u v 3 n 21 3 0
67. ts bracket inside the Celly See mechanical schematics Move the motor position horizontally to slacken or tighten the belt as needed NOTE the belt must never be too tight If so moving the turret will be too hard for the motor and mechanical defaults may occur VI11 2 Sampling assembly adjustment VIII 2 1 Needle centering M Needle When one changes the sampling needle assay it is necessary to center the needle according to the turret hole before screwing it in place Select Service menu and twist the needle until it arrive in the middle of the cuvettes Moreover check that the needle have a good position to Waste eliminate the drop VIII 2 2 Needle vertical position A lever microswitch determines the bottom and upper position of the needle Check that the needle does not hurt the side of the sink when the diluent drop is wiped away Also check that the needle does not touch the bottom of the blood samples especially control blood vials If the needle is positioned too high the dead volume in small vials will be too important The needle position can be adjusted with the small screw situated on the right side of the needle HYCEL Page 71 31 janvier 2004 CELLY Field Service Manual bioeode YCFI VIII 2 3 Needle assay notched belt adjustment If the needle does not seem to move normally or if you get mechani
68. ttes to ensure a first rinsing IV 11 Sink draining Waste pump activated V12 is activated HYCEL Page 29 31 janvier 2004 CELLY Field Service Manual MF IV 12 Summary of the first phases e Sme lT r r x 2 0 tenai needle 1 own aomm TT Al pl L p b nim T ame a m TT mle needle A A Hsmermee Tpm a own mm 1 f osm Domam lll samping ist Sampling 37m aition b m meg 0 Bemdmede 1 Waccnn ll rinsing 8 EA Firing swat ll Dmm 11 Second dilution A AL 15 6 6 A A A s E deg 1 La ur asas oR Rac avete err ext neede T x TIT Premiere dilution gt gt Waiting end of counting 7 20 HYCEL Page 30 31 janvier 2004 CELLY Field Service Manual 055777 IV 13 Counting V12 is activated e Air syringe moves down to build vacuum in the internal vacuum pressure reservoir V9 is activated to provide vacuum to the RBC orifice e V5 and V6 are activated for a very short time to generate the detection
69. uent syringe Lyse syringe Vacuum and pressure syringe Dilution cuvettes block Waste pump VI 2 Electrovalves EV1 Lyse aspiration dispense EV2 Diluent aspiration dispense EV3 Sample aspiration dispense EV4 RBC VVBC transfer EV5 Insulation of counting chambers during dilution transfers EV6 Bubble making together vvith EV5 EV7 Detergent Hemaref 11 aspiration EV8 RBC chamber draining EV9 RBC orifice vacuum pressure EV10 Internal vacuum reservoir draining EV11 WBC chamber draining EV12 Sink draining RBC chamber vacuum pressure HYCEL Page 39 31 janvier 2004 ries GM HYCFI AE bioeode R 1 T HN I CELLY Field Service Manual 5 60110101 eo oe 8118 110 uolnlip OAM 5 99 Buljdwes yndul lp u Buldues yndu 6 oBuu s 1960 moq s47 8608 71
70. ugh for the good functioning of the CELLY Serial number lt B1028 Reference B901620 Serial number gt B1028 Reference B901620 Serial number gt B1093 Reference B901620 Serial number gt B1108 Reference B901621 Serial number gt B1282 Reference B901622 Mother board PVX1 Mother board PVX3 Mother board PVX3 Mother board PVX3 CPU Intel 133 MHz and 2 Mo RAM CPU Intel 133 MHz and 2 Mo RAM CPU Intel 133 MHz and 8 Mo RAM CPU Intel 166 MHz and 8 Mo RAM Mother board PTX43 CPU Intel 166 MHz and 8 Mo RAM For each board the description of the setup program is given in the following pages HYCEL Page 44 31 janvier 2004 bioeode wiwa ny ni 4 NDTD KSTI CELLY Field Service Manual 03110 o RTS CI o lt PSS CO C Ie OS OS ZIS ZS Z lt 00 SOS 205 AS ESAS ASS SOS ESAS OS AS AS AS AS AS SOS EAS AS RES OSS OSS OS OSS ES oo N quvou L b 0 2 31 janvier 2004 Page 45 HYCEL CELLY Field Service Manual MF CELLY SN lt B1028 Pentium VX1 O Chipset d31N Md e Qi 9 1 le deyi va Io Lo 30 1861019104 5 1 15 lOd
71. y 3 partitions will be used in the final result calculation It may happen that both sequences are rejected In such a case a result is calculated but an alert S3 is displayed meaning that the result is not valid The sample should thus be re run 11 3 Hemoglobin measurement The hemoglobin sample is measured in the WBC HGB cuvette where lysing agent is added to the primary dilution A voltage ratio is derived from measuring the voltage with clear reference in the WBC cuvette and measuring the voltage with sample in that same cuvette A function of the ratio of the two measurements determines the HGB concentration Light is concentrated through the sample by use of two glass pipes HGB optical conductors contained within the WBC HGB cuvette The light from a 555 nm led passes through these light pipes and the sample to a photodetector The current generated at the output of the photodetector is proportional to the amount of light received by the detector This current is then routed to an amplifier HYCEL Page 6 31 janvier 2004 bioeode 44 HYCFI RI I TF EHMN l n RY 707 N n ST i 8 CELLY Field Service Manual I1 4 Corrections 11 4 1 Swirling back AlI cells are in the center Gauss distribution N X gt Cells not centered Assymetric distribution Red blood cells distribution Pulses histograms RBC counted as P
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