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Airstream HLF Manual (A-Series)

1. 2 21 10 Il Unpacking Your 2 13 2 1 Step by Step Procedure 13 2 2 Packaging Contents r 16 Ill Installing Your 2222 17 3 1 Choosing a Suitable 17 3 2 Environmental Electrical Condition 18 3 3 Installing Your 20 3 4 Performance Validation Certification 21 3 4 1 The importance of performance validation certification 21 3 4 2 2 21 3 4 3 22 3 4 4 References for Qualified Certifiers 22 IV Operating Your 23 4 1 Laminar Flow Control 23 4 2 Cabinet Start Up Procedure 25 4 3 Working in the Cabinet 26 4 4 Cabinet Shutdown Procedure
2. 28 4 5 DR 29 Table of Contents 1 E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com V Maintaining Your 2 2 2 30 5 1 Maintenance Schedule 30 5 2 31 B SERVICE SECTION I Maintenance by Service 32 1 1 Cabinet Balancing 32 1 2 Filter Changing 32 1 3 33 Product 222 34 2 1 Engineering Detalle ous eaten 34 2 2 Optional Retrofit uis cst i cot ien ree n 34 2 3 Technical Specification 34 2 4 Electrical EE o nae 34 Troubleshooting of Common Problems 35 Copyright 37 Warranty Registration 38 Defect
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4. 0 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com E CO AIRSTREAM HORIZONTAL LAMINAR FLOW CABINETS QUICKSTART This cabinet offers no operator protection only product protection Refer to Packaging Contents on section 2 2 and ensure that all items are received Please fill up the log record at the end of this manual Please write the first date of usage on the place provided on the pre filter N a This cabinet is capable of achieving ISO Class 3 air cleanliness within work zone as per ISO 14644 1 equivalent to Class 1 as per US Federal Standard 209E 6 Read all instructions before proceeding and observe the installation procedure and environmental electrical requirements 7 In this manual important points will be marked with this symbol 8 Choose a suitable location for your cabinet please refer to section 3 1 9 The performance of your cabinet must be validated certified before you start using them please refer to section 3 4 10 Connect cabinet to the main power supply Please leave the cabinet on for 3 minutes in order to purge airborne contamination from the work area 11 Adjust the seating position so that the user s face is above the front opening Adjustable stool is recommended 12 Prepare a written checklist of materials apparatus necessary for immediate usage and surface decontaminate the
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6. b The cabinet is secured with straps to the pallet c Remove the styrofoam and shrink wrap but leave the strap securing the cabinet to the pallet intact d If you find any damage during this stage of unpacking please refer to freight claim information on page 5 e It is the best practice to leave the cabinet secured with straps to the pallet until the cabinet is located in its approximate final position to facilitate ease and safety in handling Choosing the best location for your cabinet in order to achieve optimum operating performance of your cabinet is determined by a number of factors Please refer to the next chapter for some guidelines 5 Moving the cabinet a When lifting the pallet with the cabinet secured to it please always ensure that the floor jack or mechanical lift truck has always entered fully under the pallet This is to increase the stability of the cabinet and reduce the risk of the cabinet falling down Please use extension bar when necessary d Chapter Unpacking Your Cabinet 14 USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com b During the moving of the cabinet please ensure there is enough distance between the supports of pallet and the ground Dragging the pallet against the ground at one side or otherwise will damage the pallet and possibly your new cabinet 6 Removing t
7. separate and dedicated power outlets are needed Prior to use this cabinet should be certified by a qualified certifier Under normal operating conditions the cabinet should be recertified at least annually and when moved or serviced For more on info on performance validation certification please refer to the next section 3 4 DO NOT MOVE THE CABINET WITHOUT OBSERVING THE FOLLOWING PRECAUTIONS 1 The cabinet must be recertified after being moved 2 Observe the necessary precautions when relocating the cabinet as it is heavy va Chapter III Installing Your Cabinet 20 NE USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com 3 4 PERFORMANCE VALIDATION CERTIFICATION After installation and prior to use cabinet performance must be validated and certified to factory standards The following tests should be performed Airflow velocity Particle count test Filter leak test filter media leak test and filter gasket leak test Site installation assessment test Optional tests a Electrical safety b Light intensity test c Noise level test d UV intensity test The testing methods and equipment required are specified on the test report It is recommended that these tests be performed only by a qualified technician who is familiar with the methods and procedures for certifying laminar flow cabinets Es
8. E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com 9 American CDC Publication Primary Containment for Biohazards Selection Installation and Use of Biological Safety Cabinets Ultraviolet UV lamps are not required in BSCs Page 26 2 Personnel safety issues a Exposure to UV radiation can cause erythema of skin and eye damage b In addition the cabinet needs to be properly equipped with UV filtering materials front side covers and proper interlocking mechanism to prevent exposure which increases the overall cost and complexity of the cabinet When one considers all this additional extra precautions need to be taken plus the facts concerning the ineffectiveness of the UV lamp see below clearly the reasons for not using the UV lamp are compelling c Some UV lamps also contain mercury and breaking the lamp can result in undesirable exposure to this toxic compound d UV radiation is also reflected off some surfaces like stainless steel which means additional safety precautions need to be taken as well A front cover night door must always be utilized on a cabinet when UV is employed to prevent UV exposure of other personnel in the laboratory when the UV lamp is activated e One of the problems in working with UV radiation is that the symptoms of overexposure are not immediately felt so that persons exposed do not realize the hazar
9. ELECTRICMODULE 2 15 EP02 WITH SPEED CONTROLLER 15 EQR EL KH706 1 1 1 SPEED CONTROL 0155 075 SPEED CONTROLLER 15A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST EQR EL REL 2 1 3 RELAY JZX110 RELAY 5A 4POLE 110V EQR EL REL ic RELAY 10A 2POLE 110V 2 CAPACITOR EQR EL CCAP CBB 60 capacitor 400V 40 mf 1 40MF EQR EL TUB 4 3 FLUORESCENT TUBE 3F25W FLUORESCENT TUBE CIRCUIT BREAKER 6AMPS WITH KNURL NUT 1 4 CIRCUIT BREAKER EQR EL CB 6A FOR EL CB16A CIRCUIT BREAKER 16AMPS WITH KNURL NUT 1 EQR EL SW RL1 2 5 ROCKER SWITCH 411 ROCKER SWITCH ON OFF EQR EL SW RL1 413 ROCKER SWITCH ON OFF ON tem is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 49 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com APPENDIX A INTRODUCTION TO LAMINAR FLOW CABINET In the 1960 s due to the increased need for clean air in industry laminar flow cabinets also known as cleanbenches or laminar airflow hoods were first developed to provide product protection for small scale experimental procedures A laminar flow cabinet provides a controlled environment in which levels of particulates microbes and contamination of all kinds are regulated and kept to a minimum by constant air filtration with industrial grade f
10. Unfortunately not many service companies have the capability to check the intensity of the UV lamp and consequently the user may persist in using a lamp which has already become ineffective b The effectiveness of the UV lamp cannot be validated There does not exist a single convenient technique that is widely employed for validating the effectiveness of the UV lamp after every decontamination cycle The best option that exists is the use of a special decontamination paper strip that changes appearance after exposure to sufficient radiation but this is not employed by many cabinet users C Lack of consistency in validation technique Even when the service company may check the intensity of the UV radiation there exists a general lack of validation technique in the industry For example even the NSF49 does not have a performance test for validating this aspect of the cabinet Consequently validation technique often depends on the service company and is not reproducible throughout the industry Furthermore many service companies simply take one reading in the centre of the work zone which clearly does not suffice given the fact that the user is expecting the UV lamp to be effective in decontamination all work zone surfaces But then clearly measuring the UV intensity at all work surface positions is impracticality Appendix UV Lamps in Laminar Flow and Biological Safety Cabinet 59 EC E 21 Changi South Street 1 Singapore 486777 PHONE
11. air currents exceed the face velocity of the filter the potential exists for contaminated air to enter the work zone of the cabinet 2 A minimum distance of 50 cm to the top of the ceiling is recommended for blower changing purpose 3 A clearance of 183 cm 6ft in front of cabinet is strongly advised in order to maintain proper airflow 4 Please permit adequate space for cleaning behind the cabinet Precautions must be taken to ensure that the cabinet is put on top of a mobile platform in order to facilitate proper filter changing from the back of the cabinet us Chapter III Installing Your Cabinet 17 USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 3 2 ENVIRONMENTAL ELECTRICAL CONDITIONS This cabinet is designed to be used under the following conditions 1 Indoor use 2 Altitudes up to 2000 meters or 6600 ft 3 20 60 relative humidity 4 Temperature range from 20 deg C to 30 deg C 68 deg F to 86 deg F It is recommended that the temperature in the laboratory be maintained within 2 deg C under all conditions 5 Main supply voltage fluctuations not to exceed 10 of the nominal voltage It is recommended that the voltage fluctuation doesn t exceed 2 of the nominal voltage at all times 6 Installation Category 2 0 Installation category over voltage category defines the level of transient
12. air inlet No 3 The airflow of cabinet 3 could be influenced by the air inlet No 4 Cabinet 4 is too close to the doorway No 5 Cabinet 5 is well sited providing that the adjacent return air grille does not influence cabinet airflow The above diagram illustrates the influence on cabinet airflows from room design and ventilation systems It is not intended to depict a typical installation The location of such a number of cabinets in a small room or in close proximity is not recommended ys Chapter III Installing Your Cabinet 19 Mm 3 USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 3 3 INSTALLING YOUR CABINET Please refer to the unpacking your cabinet on the previous chapter section 2 1 step no 7 on lifting the cabinet Inspect your cabinet carefully should you find any defect please refer to the freight claim information on page 5 and our warranty terms and conditions on page 7 3 Wipe down the interior and exterior of the cabinet with water or a mild household detergent 4 Connect cabinet to the main power supply and turn on the blower Please leave the cabinet on for 3 minutes in order to purge airborne contamination from the work area Each cabinet requires its own dedicated 13A 230V or 15A 115V power outlet which should not be shared with other appliances For some cabinets which required 2 power sources 2
13. fitness for a particular purpose In the event of a defect a malfunction or failure during the warranty period Esco will repair or replace at its option the product or component therein which upon examination by Esco shall appear to be defective or not up to factory specifications The purchaser must pay any transportation or labour expenses incurred in removing and returning the product to the service centre Esco cannot be held responsible for malfunctions damage to people or property to non compliance poor or no maintenance or improper use of the cabinet This warranty shall not apply to any Esco product or part thereof which has been subject to misuse abuse accident shipping damage improper installation or service and testing or damage by fire flood or acts of God If the serial number of this product is altered removed or defaced as to be illegible the warranty shall be null and void in its entirety The use of non Esco parts and or parts not supplied directly by Esco or our authorized distributors including but not limited to maintenance parts spare parts replacement parts system components and or system accessories shall void all expressed or implied warranties To obtain warranty service the defective product or part s must be returned to Esco or an authorized Esco Factory Service Centre along with a proof of purchase It is mandatory upon purchase for the user to register receipt of the equipment with Esco online at ww
14. maintain airflow as the filter loads This unique feature eliminates the need for constant speed control adjustment and ensuring optimum performance e Light tubes are mounted out of the air stream to provide better airflow uniformity Built in solid state variable speed controller infinitely adjustable from zero to the maximum setting with built in and noise filters is superior to conventional step controllers e ISO Class 3 work zone air cleanliness as per ISO 14644 1 equivalent to Class 1 as per US Federal Standard 209E e Latest minipleat seperatorless 14 HEPA ULPA filters operating at a typical efficiency of 99 9999 at MPPS 0 3 and 0 12 microns to provide increased laminar airflow uniformity for better product and cross contamination protection CLEANABILITY e 304 grade stainless steel work top for greater cleanability e Industrial grade main body constructed for electro galvanized steel with an abrasion resistant oven baked powder coated finish to provide smooth and corrosion resistant exterior OPERATOR COMFORT The features incorporated in this cabinet allow for greater operator comfort and better working condition e 5000k fluorescent lamp provides 1200 lux of work zone lighting for less glare and greater operator comfort e Electronic ballast for fluorescent lighting resulting in lower heat output higher energy efficiency increase reliability and service life and most importantly zero flicker Chapter Basic
15. microns All HEPA filters have integral metal faceguards in order to prevent accidental damage While other manufacturers may utilize HEPA filters efficient to only 99 9796 which is the minimum requirement for a HEPA filter Esco only utilizes HEPA filters efficient to 99 999 at 0 3 microns which increases product protection by 30 times Although HEPA filters provide product protection to Class 100 levels and are the standard main filtration system of choice Esco offers customers the option of using ULPA filters instead of HEPA filters ULPA filters have an efficiency of 99 999596 at 0 12 microns and may be necessary for certain special applications with more critical requirements with regards to cleanliness and product protection General Information About HEPA ULPA Filters HEPA filters are least efficient when it comes to trapping particles of 0 3 microns in size For airborne particulates less or more than 0 3 microns in size the efficiency of the filter increases beyond its rated level Appendix A Introduction to Laminar Flow Cabinet 52 E 21 Changi South Street 1 Singapore 486777 PHONE 465 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com In order to illustrate the size of a 0 3 micron particle consider that a human hair has a diameter of approximately 100 microns whereas 10 microns is the smallest size visible to the naked human eye A micron is a measure of length that equals one mill
16. some of Esco s latest energy efficient models are used to ensure complete sterility eliminate lengthy startup times and keep the work zone ready to use at all times prevent non decontaminated biological hazards from escaping the work zone from the interior in the case of safety cabinets When this recommendation is observed the ultraviolet lamp becomes irrelevant b The ultraviolet lamp to a large extent has become largely a fallacy among users of laminar flow and biological safety cabinets today Again when one considers the way in which these lamps are used this easily becomes apparent Appendix UV Lamps in Laminar Flow and Biological Safety Cabinet 57 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com As mentioned above usually the operator will shut down the blower install a front night cover when available then turn on the UV lamp The work zone is then decontaminated or so the user believes overnight and the next day when he she returns to the lab the UV lamp is turned off and the lamps deactivated Clearly since the front night cover is not totally air tight during this interval the cabinet could already be contaminated before the user has a chance to start the fans thus rendering whatever decontamination that took place in the night useless In some extreme situations UV lamps are used without front night covers w
17. where appropriate to minimize splatter and aerosol generation in case of spillage 6 Clean materials should be at least 150 mm away from aerosol generating objects to minimize the chance for cross contamination 7 Hold lids covers above dishes sample plates in order to prevent air impingement where appropriate 8 Arrange objects to avoid airflow turbulence large objects can cause excessive turbulence in horizontal flow cabinets as illustrated below po Chapter IV Operating Your Cabinet 26 m USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com Room Air Negative Pressure Plenum Filtered Laminar Air Flow 9 Keep your arms as far away as possible from items in the work zone 10 Keep heads large potential for aerosol generation out of the work zone 11 It is recommended that the cabinet be operated continuously whenever possible to ensure and cleanliness Chapter IV Operating Your Cabinet 27 i 2 USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 4 4 CABINET SHUTDOWN PROCEDURE 1 Install front cover retrofit kits and activate UV lamp when present Eyes and skin should be exposed to direct ultraviolet light e Ultraviolet light should not be relied upon as th
18. will not know the airflow velocity Remember that a pressure gauge is not an indicator of airflow as much as manufacturers may like to lead you to believe otherwise and should not be used as such under any circumstances Esco has encountered in the industry many false recommendations such as the filters should be changed when the pressure exceeds 1 5 inches W C yes this is correct PROVIDED that airflow is maintained with an independent meter this is something hat is often unstated Other recommendations read in such a manner as to lead in our opinion the user to think of the pressure gauge as an airflow meter Increasingly pressure gauges are being replaced by true electronic airflow meters on higher end equipment such as biosafety cabinets At Esco pressure gauges have already been replaced on all higher end Esco Labculture horizontal and vertical laminar flow cabinets in addition to all biosafety cabinets Appendix The Role of the Pressure Gauge in HEPA Filtered Clean Air Devices 63 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com APPENDIX D REFERENCE MATERIALS Books and Standards Introduction to Contamination Control and Cleanroom Technology by Matt Ramstorp ISO 14644 1 Classification of air cleanliness ISO 14644 2 Specifications for testing and monitoring ISO TC 209 ISO 14644 Standards and IE
19. 20 240 1 1 ELECTRIC MODULE 270 WITH SPEED CONTROLLER 10A EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST EQR EL REL 2 1 3 RELAY x RELAY 10A 2POLE 220 240V EQR EL REL 2 4 RELAY 5A 4POLE 220 240V 2 CAPACITOR Ec CBB 60 capacitor 400V 25 mf 1 EQR EL TUB 2 FLUORESCENT TUBE 3 3 FLUORESCENT TUBE 2 FLUORESCENT TUBE 2 CIRCUIT BREAKER 6AMPS WITH KNURL NUT 4 CIRCUIT BREAKER EQR EL CB 6A EQR EL SW RL1 411 ROCKER SWITCH ON OFF 2 5 ROCKER SWITCH EQR EL SW RL1 413 ROCKER SWITCH ON OFF ON _ is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 46 ECC 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR 5 2 5 FEET 110 130V 60HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER 1524 610 10 22 2 X5 X66MM Eff gt 99 99 EQR SP FTR16 125Pa 0 45m s EQR EL BL BLOWER CENTRIFUGAL D4E 225 CC13 44 2 BLOWER D4E225CC 115V 115V TEMPERED GLASS 592 x 570 x 6MM 3 HOLES 3 TEMPERED GLASS FQR GL 22 DIA 19MM 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 110 130VAC 1 1 ELECTRIC MODUL
20. 3 HOLES 3 TEMPERED GLASS DIA 19MM 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 110 130VAC 1 1 ELECTRIC MODULE 02 WITH SPEED 10 EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST EQR EL REL 2 1 3 RELAY JZX110 RELAY 5A 4POLE 110V EQR EL REL 10 110 RELAY 10A 2POLE 110 5 2 CAPACITOR uL PT CBB 60 capacitor 400V 40 mf 1 EQR EL TUB 3 3 FLUORESCENT TUBE 2F17W FLUORESCENT TUBE 2FT CIRCUIT BREAKER 6AMPS WITH KNURL NUT 1 4 CIRCUIT BREAKER EQR EL CB 6A EQR EL CB 10A CIRCUIT BREAKER 10AMPS WITH KNURL NUT 1 2 EQR EL SW RL1 ROCKER SWITCH ON OFF 411 5 ROCKER SWITCH 413 ROCKER SWITCH ON OFF ON tem is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 41 ECC 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR AHC 3A1 3 FEET 220 240V 50HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER 914 610 10 22 2 X3 X66MM gt 99 99 EQR SP FTR14 ab 125 0 45m s 1 EQR EL BL e BLOWER CENTRIFUGAL 04 200 TEMPERED GLASS 592 x 570 x
21. 4E200CC 230V BLOWER CENTRIFUGAL D4E 200 GLASS 592 x 570 x 6 3 HOLES 3 GLASS EQR GL 22 DIA 19MM 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 220 240 1 1 ELECTRIC MODULE EP01 WITH SPEED CONTROLLER 10A EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST TP4 321S ELECTRONIC BALLAST EQR EL REL 2 13 RELAY JOX220 RELAY 10A 2POLE 220 240V EQR EL REL 2 JZX220 RELAY 5A 4POLE 220 240V 2 CAPACITOR B e CBB 60 capacitor 400V 25 mf 1 EQR EL TUB 3 FLUORESCENT TUBE 2F17W FLUORESCENT TUBE 2FT 3 CIRCUIT BREAKER 6AMPS WITH KNURL NUT 2 4 CIRCUIT BREAKER EGR EL CB 6A EQR EL SW RL1 2 411 ROCKER SWITCH ON OFF 5 ROCKER SWITCH FOR ELSW ALT 413 ROCKER SWITCH RL ON OFF ON Item is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 40 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR AHC 2A2 2 FEET 110 130V 60HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER 610 610 10 22 2 X2 X66MM gt 99 99 EQR SP FTR12 ab 125Pa 0 45m s EQR EL BL 2 D4E225CC 115V BLOWER CENTRIFUGAL D4E 225 115V 1 EQR GL 22 TEMPERED GLASS 592 x 570 x 6MM
22. 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com APPENDIX C THE ROLE OF THE PRESSURE GAUGE IN HEPA FILTERED CLEAN AIR DEVICES Abstract HEPA filtered clean air devices systems containing both a fan and a HEPA filter are prevalent today in many modern applications They include laminar flow biological safety cabinets hoods for laboratory applications in addition to cleanroom equipment such as fan filter units This technical paper outlines the theory of operation of the pressure gauge which is commonly used in the industry as an indicator on HEPA filtered clean air devices are discussed in detail Proper usage practices are also recommended Introduction and Theory of Operation The pressure gauges found on all HEPA filtered clean air devices are at the most fundamental level indicators of differential pressure In a HEPA filtered clean air device for air to move across a HEPA filter there must be a difference between the pressures of the plenums and enclosed air space in which the pressure at multiple points is almost uniform before and after the filter Air will then move from a region of higher pressure across the filter to a region of lower pressure A fan is used to create this pressure difference For example a fan may be used to pressurize air positively with respect to the ambient environment in a plenum Air is then forced from a region of high pressure before the filter acro
23. 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com International Standards and Norms The most common standard referred to by most manufacturers of laminar flow systems is the US Federal Standard 209E It is important to understand that the 209E does not apply specifically to laminar flow cabinets and that is only applies in general to air cleanliness for cleanrooms The US Federal Standard 209E does not deal with general aspects of laminar flow cabinet construction However it deals with the most important aspect of cabinet performance which is the level of product protection provided in other words the cleanliness of the air within the working area of the laminar flow cabinet Most laminar flow cabinets meet Class 100 with HEPA filters or Class 10 with ULPA filters standards This is the reason that Esco specifically declares that the clean working area provided within our cabinet complies to the US Federal Standard 209E for Class 100 cleanliness Other standards and norms similar to the US Federal Standard 209E dealing with cleanrooms and clean air are the BS 5295 AS 1386 VDI 2083 and the recently released ISO standard 14664 The main filtration process in all Esco laminar flow cabinets comprises of a HEPA high efficiency particulate air filter that removes airborne contaminants including all particulate matter bacteria and spores with an efficiency of 99 999 rated at a particle size of 0 3
24. 6MM 3 HOLES 3 TEMPERED GLASS EQR GL 22 DIA 19MM 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 220 240VAC 1 1 ELECTRIC MODULE FROITO EP01 WITH SPEED CONTROLLER 10A EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST TP4 3218 UNIVERSAL EQR EL REL x RELAY 10A 2POLE 220 240V EQR EL REL 1 RELAY 5A 4POLE 220 240V 5 2 CAPACITOR c CBB 60 capacitor 400V 25 mf 1 EQR EL TUB 2 3 FLUORESCENT TUBE 3F25W FLUORESCENT TUBE 3 FT CIRCUIT BREAKER ZE 700 6 6AMPS WITH 2 4 EQR EL CB 6A KNURL NUT EQR EL SW RL1 2 5 ROCKER SWITCH 411 ROCKER SWITCH ON OFF EQR EL SW RL1 413 ROCKER SWITCH ON OFF ON tem is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List A2 ECC 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR AHC 3A2 3 FEET 110 130V 60HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER 914 610 10 22 2 X3 X66MM gt 99 99 1 EQRISP FTR14 125 0 45m s EQR EL BL BLOWER CENTRIFUGAL 04 225 13 44 2 PLOWER D4E225CC 115V 115V TEMPERED GLASS 592 x 570 x 6 3 HOLE
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26. Changi South Street 1 Singapore 486777 Tel 65 65420833 Fax 65 65426920 hereby declare on our sole responsibility that the product Model Airstream Horizontal Laminar Flow Cabinet has been designed and manufactured to conform with the following standards when installed and used in accordance with the operating and maintenance manual EN 50081 1 1992 Emissions 55022 Class A Conducted Emissions e EN61000 3 2 3 Harmonic Current Emissions and Voltage Fluctuations 55022 Class A Radiated Emissions EN 50082 1 1997 Immunity EN 61010 1 1997 Electrical Equipment Safety Requirements This declaration is valid also as declaration of conformity to the following directives 73 23 EEC Directive on low voltage 89 336 Directive on electromagnetic compatibility 93 68 EEC Directive on CE Marking 98 37 Directive on machinery More information may be obtained from Esco s authorised distributors located within the EU and a list of these parties and their necessary contact information is available on request from Esco Lim Lay Yew Chief Executive Officer Applicable only to 230V 50 Hz cabinets Declaration of Conformity 4 EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com FREIGHT CLAIM INFORMATION Esco inspects each product for defects before shipment Esco products are th
27. E EP02 H10 EP02 WITH SPEED CONTROLLER 10A EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST EQR EL REL 2 1 3 RELAY JZX110 RELAY 5A 4POLE 110V EQR EL REL RELAY 10A 2POLE 110 2 iu e CBB 60 capacitor 400V 40 mf 1 FLUORESCENT TUBE F25T8 850 2 3 FLUORESCENT TUBE EQR EL TUB FLUORESCENT 2 CIRCUIT BREAKER 6AMPS WITH KNURL NUT 1 4 CIRCUIT BREAKER EQR EL CB 6A FQRIEL CB 10A CIRCUIT BREAKER 10AMPS WITH KNURL NUT 1 EQR EL SW RL1 2 5 ROCKER SWITCH 411 ROCKER SWITCH ON OFF EQR EL SW RL1 413 ROCKER SWITCH ON OFF ON tem is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 47 ECC 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR AHC 6A1 6 FEET 220 240V 60HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER 610X1829X66 ULPA W LAMINATOR gt 99 99 EQR SP FTR17 dP 125Pa 0 45m s EQR BL D4paso BLOWER D4E250 230V 230 TEMPERED GLASS 592 x 570 x 6 3 HOLES 3 TEMPERED GLASS EQR GL 22 DIA 19 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 220 240VAC 1 1 EL
28. ECTRICMODULE EPOTH10 EP01 WITH SPEED CONTROLLER 10A EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST EQR EL REL 127 RELAY 10A 2POLE 220 240V EQR EL REL 2 RELAY 5A 4POLE 220 240V E 2 CAPACITOR EQR EL CCAP CBB 60 capacitor 400V 25 mf 1 25MF EQR EL TUB FLUORESCENT TUBE F25T8 850 4 CIRCUIT BREAKER 6AMPS WITH KNURL NUT 1 4 CIRCUIT BREAKER EQR EL CB 6A CIRCUIT BREAKER 10AMPS WITH KNURL NUT 1 EQR EL SW RL1 2 5 ROCKER SWITCH 411 ROCKER SWITCH ON OFF EQR EL SW RL1 413 ROCKER SWITCH ON OFF ON is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List A8 ECC 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR AHC 6A2 6 FEET 110 130V 60HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER 610 1829 66 ULPA W LAMINATOR gt 99 99 1 EQR SP FTR17 ab 125 0 45m s EQR EL BL 2 BLOWER sy BLOWER CENTRIFUGAL 04 225 115V 1 GLASS 592 x 570 x 6 3 HOLES 3 TEMPERED GLASS EQR GL 22 DIA 19MM 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 110 130VAC 1 1
29. EMENT PARTS COMPLETE ELECTRICAL PANEL 110 130VAC 1 MODULE EP02 H10 EP02 WITH SPEED CONTROLLER 10A EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST EQR EL REL 2 1 3 RELAY JZX110 RELAY 5A 4POLE 110V EQR EL REL 11 RELAY 10A 2POLE 110V 2 CAPACITOR 2 CBB 60 capacitor 400 40 mf 1 EQR EL TUB 2 3 FLUORESCENT TUBE 4F32W FLUORESCENT TUBE CIRCUIT BREAKER 6AMPS WITH KNURL NUT 1 4 CIRCUIT BREAKER EQR EL CB 6A CIRCUIT BREAKER 10AMPS WITH KNURL NUT 1 EQR EL SW RL1 2 411 ROCKER SWITCH ON OFF 5 ROCKER SWITCH 413 ROCKER SWITCH ON OFF ON tem is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 45 ECC 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR AHC 5A1 5 FEET 220 240V 50HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER 1524 610 10 22 2 X5 X66MM gt 99 99 EQR SP FTR16 dP 125 0 45m s EQR EL BL Soap D4t 00CC BLOWER CENTRIFUGAL D4E 200 i TEMPERED GLASS 592 x 570 x 6MM 3 HOLES 3 TEMPERED GLASS EQR GL 22 DIA 19MM 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 2
30. ES 3 TEMPERED GLASS EQR GL 22 DIA 19 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 220 240VAC 1 1 ELECTRIC MODULE EP01 WITH SPEED CONTROLLER 10 EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST TP4 321S ELECTRONIC BALLAST EQR EL REL 2 1 3 RELAY JOX220 RELAY 10A 2POLE 220 240V EQR EL REL JZX220 RELAY 5A 4POLE 220 240V 2 CAPACITOR 2 CBB 60 capacitor 400V 25 mf 1 EQR EL TUB 2 3 FLUORESCENT TUBE 4F32W FLUORESCENT TUBE 4 CIRCUIT BREAKER 6AMPS WITH KNURL NUT 2 4 CIRCUIT BREAKER EQR EL CB 6A EQR EL SW RL1 2 5 ROCKER SWITCH 411 ROCKER SWITCH ON OFF EQR EL SW RL1 413 ROCKER SWITCH ON OFF ON tem is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 44 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR 4 2 4 FEET 110 130V 60HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1219 610 11 22 2 X4 X66MM gt 99 99 1 FILTER EQRISP ETRI5 125Pa 0 45m s 1 EQR EL BL 2 BLOWER BLOWER CENTRIFUGAL 04 225 1 GLASS 592 x 570 x 6 3 HOLES 3 TEMPERED GLASS EQR GL 22 DIA 19MM 2 ELECTRICAL REPLAC
31. HAPTER II PRODUCT SPECIFICATION 2 1 ENGINEERING DETAILS 4 PLEASE KINDLY REFER TO THE END OF THIS SECTION 2 2 OPTIONAL RETROFIT K ITS 1 gt Support Stand WARNING 2 persons are required to assembly the support stand as it is heavy To enable mobility of the cabinet within the laboratory and for users without bench space Types available a Standard with castors b With levelling feet IV Bar with 6 hooks For IV bags filling applications Service Fixtures both European and American style fixtures available To provide supplies of gas vacuum water and compressed air to the cabinet For vacuum fixture please install a filter between the work zone and the fixture Germicidal UV Lamp For germicidal decontamination Electrical outlets total 6A rating for all outlets in cabinet To allow users to use electrical devices such as ultra sonic bath hotplate and electronic weighing scale inside the work zone Front cover also known as Night Doors in some countries To protect operator from harmful UV rays and also to keep work zone free from dust Ergonomic footrest Ergonomic adjustable lab chair 2 3 TECHNICAL SPECIFICATION PLEASE KINDLY REFER TO THE END OF THIS SECTION 2 4 ELECTRICAL DIAGRAM PLEASE KINDLY REFER TO THE END OF THIS SECTION Chapter Product Specification 34 SERVICE SECTION LEGEND 1 GLASS SIDE PANEL ULPA FILTER PRE FI
32. L biotech escoglobal com WEBSITE www escoglobal com Replace the capacitor Check for open wiring connection Faulty speed control 1 Short out control by placing jumper wire directly across the connections to the two speeds control leads 2 If the fan operates the speed control is defective Call your distributor for replacement 3 If the fan does not run the speed control is not the problem Excessive fan noise Loose Motor or Blower Wheel Mount Check the tightness of the setscrew holding the blower wheel Check the tightness of all blower and motor mounting bolts Light always Faulty relay Check K2 and K3 relay on the electrical panel OFF Ensure that C NC connection is closed and C NO open Replace the faulty relay Call your distributor for details Faulty light Replace the faulty light UV Light Faulty relay Check K2 and K4 relay on the electrical panel always OFF Ensure that C NC connection is closed and C NO open Replace the faulty relay Call your distributor for details Faulty light Replace the faulty light Contaminated Filter leaking Repair or replace filter and recertify the cabinet samples Low airflow Readjust the airflow recertify the cabinet 9 A Chapter HI Troubleshooting of Common Problems 36 SERVICE SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com C
33. LTER BLOWER FLUORESCENT LAMP STANDARD IV BAR RETROFIT KIT PROVISION FRONT COVER OPTIONAL STANDARD UN LIGHT RETROFIT KIT SYSTEM PLUG PENETRATION 2 HOLES ON EACH SIDE 10 PRESSURE GAUGE 11 OPERATING SWTCHES 12 SIDE TEMPERED GLASS 13 STAINLESS STEEL WORK TOP VIEW INSIDE WORK AREA CEILING 14 STANDARD ESCO ELECTRICAL OUTLET RETROFIT KIT PROVISION 2 amp 3 FT MODEL ONLY 1 SINGLE OUTLET IN WORK ZONE CEILING LEFT SIDE 4 amp FT MODEL ONLY 2 SINGLE OUTLET IN WORK ZONE EILING LEFT amp RIGHT SIDE 730 1035 1340 1645 DRAWN CHECKED DRAWN SCALB RANDY PRINTED NTS AIRSTREAM HORIZONTAL _ DATE LAMINAR FLOW CABINET 20 8 2003 149 8 12 Sp we 79 X V 28 X 128 we VO X V 607 we 79 X V 28 X 0 69 VO X V 4 27 we VO X 96 06 1 X 096 X 0012 0691 X 096 X 0081 0591 X 096 X 0091 0591 X 0S6 X 0021 0891 X 046 X 006 sql 999 567 62 sql egg 567 62 26 567 567 002 90v 56 981 sql 56 802 992 567 91 541662 56 272 567 2 L 202 567 26 V88 L f118980S 10 9991 1 66040 8 8 911 188262 10 A8S8 V 0Z f118188c 10 8 9 1189282 10 1808 60 4 199989 10 MIE9L 9 19791 10 9 92 1 960 40 9 19 2 10 M009 VrS 2 1188661 10 AF8S 199 9 86 uq 6 72 199 6718
34. Ltd us Chapter III Installing Your Cabinet 22 USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com CHAPTER IV OPERATING YOUR CABINET 4 1 LAMINAR FLOW CONTROL SYSTEM UV Button epo rj Light Button Socket Button Fan Button Pressure Gauge Fig 8 Laminar Flow Control System 1 Fan Button To turn on and turn off the fan 2 Light Button To turn on the light 3 UV Button To turn on the UV retrofit kit 4 Socket Button To turn on and turn off the electrical socket retrofit kit Chapter IV Operating Your Cabinet 23 2 USER SECTION 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com It is able to undertake AC powered device up to 5 ampere In case of overloading the outlet the circuit breaker will trip Disconnect the device from the outlet and reset the circuit breaker by pressing its button 5 Pressure Gauge To indicate the pressure drop across filter to maintain nominal airflow velocity as specified inside the test report For more information on pressure gauge please refer to Appendix C Chapter IV Operating Your Cabinet 24 USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech esco
35. OPYRIGHT INFORMATION Copyright 2004 Esco Micro Pte Ltd All rights reserved Esco Micro Pte Ltd 21 Changi South Street 1 Singapore 486777 The information contained in this manual and the accompanying product are copyrighted and all rights are reserved by Esco Micro Pte Ltd Esco Micro Pte Ltd reserves the right to make periodic minor design changes without obligation to notify any person or entity of such change Airstream is a registered trademark of Esco Micro Pte Ltd Copyright Information 37 EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com WARRANTY REGISTRATION FORM Thank you for purchasing an Esco product To be applicable for the latest product promotions and special offers please complete this warranty registeration form and return it to Esco by fax or postal mail PRODUCT INFORMATION Model Number Serial Number Date of Purchase CUSTOMER INFORMATION Company Address Telephone Fax Email NAME OF ESCO DISTRIBUTOR Contact Company Country YOUR COMMENTS Please Fill in Your T Shirt Size and Get a FREE Esco T Shirt Allow Up to 4 Weeks For Delivery T Shirt Size Large Extra Large Delete Inapplicable Field Warranty Registration Form 38 EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www
36. PA filtered clean air devices the pressure gauge only reads in one direction i e its value cannot be negative When pressure gauge is present on a HEPA filtered clean air device it indicates pressure as follows one sampling tube will be connected to the plenum just before the filter and the other sampling tube will be connected to the plenum just after the filter The gauge then indicates the pressure difference between both plenums In the case of a laminar flow cabinet for example a sampling tube will be connected to the positive pressure plenum just before the filter and the other tube connected to the exterior of the cabinet to sample ambient pressure In the case of Esco laminar flow cabinets the second tube is connected to the region of negative pressure that is before the fan but after the pre filters Hence on Esco cabinets the pressure gauge indicates the pressure difference not only across the main HEPA filter but across the entire fan system taking into consideration the drop across the pre filters Differential pressure gauges also called a manometer are available in different display units Common display units include inches of water millimeters of water or Pascal s The most common pressure gauge suitable for indicating the relatively low differential pressures present on HEPA filters is unequivocally the Minihelic Magnehelic series of gauges manufactured by Dwyer Instruments Inc Esco products make use of a gauge disp
37. Product Information 11 a USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com Extremely low noise and vibration levels less than 59 at working position due to proprietary construction and mounting technology An aerosol DOP PAO challenge test port is included e Germicidal UV lamp is placed out of operator s direct line of sight EASE OF SERVICE e Lifting handles for filter and blower maintenance on the back cover e Convenient hand tool kit and extra fasteners is supplied with every cabinet COST SAVING e Permanently lubricated direct drive centrifugal blower energy efficient external rotor type design Please refer to chapter 2 of SERVICE SECTION for detailed product specification Each individual unit shipped is extensively tested for performance and safety and delivered with a detailed test report and certificate of performance Testing performed at our factory laboratory includes Airflow velocity e Operator comfort tests noise light Filter integrity PAO aerosol challenge Particle counting for air cleanliness to ISO14644 e Electrical safety to IEC61010 1 Additional units are also randomly selected on a statistical sampling basis and re tested using research grade instrumentation and additional test protocols at our dedicated Product Development Laboratory Esco performs testing in accordance wi
38. Reporting 2222 2 2 4 24242 2 39 Replacement Parts 40 APPENDIXES Appendix A Introduction to Laminar Flow 50 Appendix B UV Lamps in Laminar Flow and Biological Safety Cabinet 54 Appendix C The Role of the Pressure Gauge HEPA Filtered Clean Air Devices 60 Appendix D x aa exon ura Fea d dii ab va a 64 Table of Contents 2 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com WARNING This cabinet offers no operator protection only product protection This cabinet is capable of achieving ISO Class 3 air cleanliness within work zone as per ISO 14644 1 equivalent to Class 1 as per US Federal Standard 209E Read all instructions before proceeding and observe the installation procedure and environmental electrical requirements In this manual important points will be marked with this symbol Warning 3 EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com Declaration of Conformity In accordance with EN 45014 1998 We Esco Micro Pte Ltd of 21
39. S 3 TEMPERED GLASS EQR GL 22 DIA 19MM 2 ELECTRICAL REPLACEMENT PARTS COMPLETE ELECTRICAL PANEL 110 130VAC 1 1e ELESTRIC MODULE 2 WITH SPEED CONTROLLER 10A EQR EL KH706 1 1 1 SPEED CONTROL 0155 060 SPEED CONTROLLER 10A EQR EL BAL 1 1 2 BALLAST 4 3215 ELECTRONIC BALLAST EQR EL REL 2 1 3 RELAY JZX110 RELAY 5A 4POLE 110V EQR EL REL jodi RELAY 10A 2POLE 110V 2 du ee CBB 60 capacitor 400V 40 mf 1 EQR EL TUB 2 3 FLUORESCENT TUBE 3F25W FLUORESCENT TUBE SFI CIRCUIT BREAKER 6 5 WITH KNURL NUT 1 4 CIRCUIT BREAKER EQR EL CB 6A CIRCUIT BREAKER 10AMPS WITH KNURL NUT 1 EQR EL SW RL1 2 5 ROCKER SWITCH 411 ROCKER SWITCH ON OFF EQR EL SW RL1 413 ROCKER SWITCH ON OFF ON tem is included on complete main replacement electrical panel However it may be replaced individually if required Replacement Parts List 43 ECC 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com PARTS LIST FOR AHC 4A1 4 FEET 220 240V 50HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER _ 1219 610 11 22 2 X4 X66MM gt 99 99 EQR SP FTR15 ab 125 0 45m s EQR EL BL 2 BLOWER D4E200CC 230V BLOWER CENTRIFUGAL D4E 200 i TEMPERED GLASS 592 x 570 x 6MM 3 HOL
40. ST Technical Guides to the ISO Standards ISO DIS 14644 7 Separative enclosures IEST G CC1001 IEST technical guide for counting airborne particles for classification oa N and monitoring of cleanrooms and clean zones 7 IEST G CC1002 IEST technical guide for determination of the concentration of airborne ultra fine particles 8 IEST G CC1003 IEST technical guide for measurement of airborne macro particles 9 IEST G CC1004 IEST technical guide for sequential sampling plan for use in classification of the particulate cleanliness of air in cleanrooms and clean zones 10 IEST RP CC001 3 IEST recommended practices for HEPA and ULPA filters 11 IEST RP CC007 1 IEST recommended practices for testing ULPA filters 12 IEST RP CC021 1 IEST recommended practices for testing HEPA and ULPA filter media 13 IEST RP CC034 1 IEST recommended practices for HEPA ULPA filter leak tests 14 EN1822 Parts 1 to 5 European air filters standard 15 EN 13091 Biotechnology Performance criteria for filter elements and filtration assemblies 16 EN 112741 Biotechnology Laboratories for research development and analysis 17 EN 12296 Biotechnology Equipment Guidance on testing procedures for cleanability 18 EN 12297 Biotechnology Equipment Guidance on testing procedures for sterilizability 19 EN 12298 Biotechnology Equipment Guidance on testing procedures for leak tightness 20 AS 1386 Cleanrooms and Cl
41. aboratory with a history of quality cabinets since 1978 We are predominantly oriented towards the international marketplace with sales in more than 60 countries A network of international distributors and partners allows us to provide quick and responsive local service and support Today Esco s production capabilities for our laboratory equipment products are centralized in a 3 900 m 2 42 000 sq ft facility separate from our Singapore corporate headquarters This state of the art fabrication centre houses the latest CNC computer numerically controlled sheet metal machinery and is highly automated to ensure that all products produced are of a high quality with less human intervention An advanced powder coating process is also operated in house to ensure an aesthetic finish on all Esco laboratory equipment products Esco is committed to making continuous investments in fabrication technologies to ensure superior quality and total customer satisfaction Corporate Profile Biotechnology Equipment Division 8 EC e 0 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 amp E MAIL biotech escoglobal com WEBSITE www escoglobal com Fig 2 Esco Production Facility At Esco continuous research and development is a corporate policy realized through tangible investments in personnel equipment and facilities Currently 1 out of every 2 non production staff at Esco works in a research and development position Visi
42. ace dust on the glass surface of the lamp for maximum effectiveness but this aspect is often ignored by users which therefore degrades the performance of the UV lamp 5 The UV lamp encourages bad working practice Appendix UV Lamps in Laminar Flow and Biological Safety Cabinet 58 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com The lamp encourages bad working practice by giving users a false sense of security While manufacturers like Esco constantly emphasize that when the lamp is used it is ONLY an aid to surface decontamination some users lulled into a sense of security may disregard usual surface decontamination practices In other words before using the cabinet they believe the work zone is clean and do not wipe down with for example 7096 alcohol After using the cabinet they also disregard the wipe down process which is especially important on biological safety cabinets When one considers that the UV lamp for the reasons stated above is ineffective this work habit or the lack thereof and false sense of security that the UV lamp encourages in users is detrimental to the performance of the cabinet 6 Lack of validation a UV lamps are not changed when they have to As mentioned above UV lamps have a specific life cycle and need to be changed after a certain number of hours to maintain effectiveness radiation intensity
43. ause corrosion to steel and stainless steel resulting in irreparable damage to the cabinet structure Do surface decontamination as well to the UV lights and electrical outlet when present Minimize room activity personnel movements closing and opening of doors etc Please refer to installation guidelines on section 3 1 Chapter IV Operating Your Cabinet 25 0 USER SECTION c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 4 3 WORKING IN THE CABINET 1 Work from clean to dirty by following the segregation of clean and dirty materials apparatus as described in the start up procedure on top 2 Particularly critical objects may be arranged such that air flows directly against them 3 Do not use a gas flame whenever possible as it interferes with airflow Do not use writing materials inside work area as they generate aerosols e not change the cabinet original blower speed unless the change is required by a decrease in measured air velocity Adjustment should be made only by a qualified technician e not operate the cabinet if fan fails to run not cough or sneeze into the work zone 4 Minimize arm movement Move arms in and out of the cabinet slowly to avoid disrupting cabinet airflow Be sure to surface decontaminate first when inserting arms into the cabinet 5 Use absorbent pads on the work surface
44. button Motor silent Blown fuse Switch off the cabinet Faulty relays 1 Switch off the cabinet 2 Check K1 relay on the electrical panel Ensure that C NC connection is closed and C NO open 3 Replace the faulty relay s Faulty speed 1 Short out control by placing jumper wire directly control across the connections to the two speeds control leads 2 If the fan operates the speed control is defective Call your distributor for replacement 3 the fan does not run the speed control is not the problem Auto thermal cut off 1 Blower was overheated 2 Wait for 30 minutes and try again Ensure that hazardous materials inside the cabinet Motor failure 1 Disconnect two motor leads at the motor Connect a proper AC voltage source directly to the motor 2 motor starts to run the problem is in the wiring connection Refer to blown fuse or faulty relay section 3 the motor does not run the motor is defective Replace motor Faulty capacitor Replace the capacitor Check for open wiring connection Inoperative Connection Check whether the cable from electrical panel has switches problem been connected properly to the switch Low airflow Low speed control Increase the speed control setting until motor starts setting 9 A Chapter III Troubleshooting of Common Problems 35 SERVICE SECTION ESCO Faulty capacitor 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAI
45. co conducts training courses on the validation and certification of laminar flow cabinets If you are interested you are most welcome to contact your distributor or Esco 3 4 1 THE IMPORTANCE OF PERFOMANCE VALIDATION CERTIFICATION e Airflow velocity value that falls below the value specified inside the test report will not provide adequate product protection e Possible product contamination when the filter is leaking 3 4 2 RECERTIFICATION After the initial certification the cabinet shall be recertified at the following situations 1 Relocation of cabinet 2 When the performance is suspected 3 Major maintenance or service filter changing blower changing etc 4 At least annually va Chapter III Installing Your Cabinet 21 2 USER SECTION 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 3 4 3 DISCLAIMER The perfomance of the cabinet while rigorously evaluated at the factory cannot be guaranted once after transit and installation Therefore the on site testing is always recommended 3 4 4 REFERENCES FOR QUALIFIED CERTIFIERS 1 NSF Accredited Class Il Biosafety Cabinet Field Certifiers Please see http www nsf org Certified Biosafety Certifier for more information 2 member certifying company Please see http www iafca com listview html for more information 3 Your distributor 4 Esco Micro Pte
46. com Practically though the pressure gauge has some useful applications On Esco cabinets the initial operating pressure corresponding to the required airflow setpoint is recorded at the factory during initial commissioning If the setting on the variable speed control has been accidentally reset or adjusted before actual use the user may upon actual use adjust the variable speed control so as to operate the filters at the same pressure point In general the pressure gauge can also indicate very roughly airflow across the filter On a new filler an operating point of 125 pa or 0 5 W C corresponds to a filter velocity of roughly 0 45m s or 90fpm Although this operating pressure point may increase as the filter loads under all conditions an increase in pressure across the filter in the same operating health condition correspond to an increase in airflow The pressure gauge may hence be used as a relatively inaccurate airflow meter that is true in itself In pharmaceutical applications to meet GMP requirements a pressure gauge is useful from the point of view that is allows the user to record operating conditions in terms of the pressure loss across the filter In many cases a pressure gauge is the only airflow meter available economically and may be thought of as better than nothing although it is far from ideal If there is a pressure loss across the filter the user knows that there is airflow across the filter although he or she
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48. d until after the damage is done f Germicidal UV lamps produce ozone which can also be toxic in high concentrations 3 Performance degradation of the cabinet a Ultraviolet radiation can potentially degrade materials in the cabinet such as certain plastics when they may be used and sealant such as RTV silicone used to ensure joints seams are airtight In the most extreme dangerous situation for biological safety cabinets this could cause critical joints to degrade in turn resulting in other performance issues loss of pressure tightness integrity potentially allowing biological hazards to escape through these seams or allowing contamination to accumulate in these joints posing a cleanability decontamination problem Appendix UV Lamps in Laminar Flow and Biological Safety Cabinet 56 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com While Esco ensures that all materials in our cabinets are resistant to ultraviolet radiation this may not be the case for other manufacturers In addition clearly by eliminating this extra performance variable i e not using an ultraviolet light especially after considering the other detrimental effects of the UV lamp the long term overall performance of the cabinet can be better ensured b Airflow interference and turbulence When UV lamps are installed as a permanent fixture in the work zone w
49. e filter and also guarantees the uniformity of airflow within the working zone of the cabinet which helps to enhance product protection Laminar airflow guarantees that air throughout the cabinet is replaced in a consistent fashion This also ensures that particles generated in the cabinet due to normal work processes do not become trapped in dead air corners where they may otherwise accumulate and cause product contamination Laminar Flow Cabinets Provide Product Protection Product or sample protection is important in a wide variety of modern industries This includes the pharmaceuticals industry in which clean air is necessary for the sterile production of drugs In the semiconductors and microelectronics fields a single dust particle can cause irreversible product damage during the manufacturing process which is why clean air is necessary to protect sensitive components without which the miniaturization of electronics would otherwise be impossible In the laboratory airborne contamination can often influence experimental procedures and results which is why processes should be carried out under controlled environment conditions An Esco Laminar Flow Cabinet equipped with a state of the art filtration system can reduce airborne contamination levels greatly within the working area depending on the filtration system of choice Appendix A Introduction to Laminar Flow Cabinet 51 EC E 21 Changi South Street 1 Singapore 486777 PHONE
50. e sole decontaminating agent e Check UV interlock regularly for correct operation The use of UV lamp in laminar flow cabinets is explicitly discouraged in all major international standards and recommendations Please refer to Appendix B for more information 2 Turn off the cabinet remove laboratory coat and gloves and wash hands using germicidal soap thoroughly ya Chapter IV Operating Your Cabinet 28 j USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 4 5 ERGONOMICS During the operation of your cabinets you will be more likely to do it in the sitting position Advantages of sitting position 1 The physiological energy cost and fatigue are reduced 2 It provides the body with a stable support However sitting position also has some drawbacks 1 Limited working area 2 Possible risk of being constrained in a fix posture for a long time 3 One of the most stressful position for the back Fortunately it is possible to create a more comfortable and healthier working condition by following these simple steps 1 Ensure there is enough space for your leg and feet 2 Keep the lower back comfortably supported by your chair Adjust the chair or use a pillow behind your back when necessary 3 Position your fleet flat on the floor or a footrest Don t dangle your feet and compress your thighs 4 Vary your sitting p
51. ean Workstations 21 IEST RP CC002 2 Unidirectional Flow Clean Air Devices Appendix D Reference Materials 64 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com Websites 1 NSF Accredited Class Il Biosafety Cabinet Field Certifiers Please see http www nsf org Certified Biosafety Certifier for more information 2 A F C A member certifying company Please see http www iafca com listview html for more information 3 www escoglobal com Esco Micro Pte Ltd Appendix D Reference Materials 65 Log Record Installed By Date Cabinet Model Company Serial Number Responsible Person Please record any major maintenance procedure performed by the technician and or operator for example filter changing recertification uv lamp replacement pre filter replacement speed control adjustment etc Date Event Operator Manager _ Signature Signature Operator Manager xdi ent Signature Signature Operator Manager xdi ent Signature Signature Operator Manager Date Event Signature Signature ECCO 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com Visit our website at www escoglobal com
52. ed pieces at no charge if the original order was shipped under CIF terms i e Esco had covered the insurance NOTE Any correspondence with Esco regarding loss or damage must be accompanied by a copy of the shipping carrier s report Esco will not accept returns that have not been authorized In the event of accepted freight with damage or loss notification of loss or damage must be sent to the carrier within 10 days of receiving the freight Notification outside the 10 day time frame may result in shipping damage claim being denied Freight Claim Information 6 EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com WARRANTY TERMS AND CONDITIONS Esco warrants that this equipment will perform according to the specifications for 3 years from the date of purchase With exceptions noted below this 3 years warranty applies to all parts and components of this equipment 3 years except consumable items filter light tubes UV tubes pre filters Esco s liability under this warranty shall be limited to repair or replacement of the equipment at Esco s option and under no circumstances shall Esco be liable for the equipment damage arising out of or in any way connected with the failure of the equipment to perform as set forth herein This limited warranty is in lieu of all other expressed or implied warranties including those of merchantability and
53. en carefully packed in compliance with carrier regulations and thoroughly inspected before leaving our plant Responsibility for their safe delivery is assumed by the carrier upon acceptance of the shipment Occasionally damage occurs in transit Claims for loss or damage sustained in transit must be made upon the carrier Please remember that you are responsible for all freight claims and the cost of all replacement pieces for each shipment you accept Inspect each shipment very carefully before acceptance Carefully inspect each pallet or crate upon arrival If a shipment is found to be damaged upon delivery be sure to have the driver carrier note all damage details on the delivery receipt This is essential or your claim may be denied Also if pallets are stacked please note Stacked Pallets on the delivery receipt pallets are not stacked when shipped unless otherwise stated for certain products Esco is not responsible for pallets stacked at carrier terminal Any unloading difficulties or damages due to stacked materials are carrier s responsibility If freight damage is discovered please refer to the following guidelines in order to process and effective freight claim ACCEPTED FREIGHT WITH NOTED VISIBLE LOSS OR DAMAGE Any external evidence of loss or damage must be noted on the freight bill or delivery receipt and signed by the courier s agent or delivery driver NOTE Failure to properly describe evidence of loss or damage may
54. erator Manager Month Clean exterior surface Check all service fixtures Signature Signature Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Daily Surface decontamination Quarterly Replace pre filters Annually 1 Re certification 2 Change UV lamp when present Bi anually Replace fluorescent lamps This form may be used by the lab manager safety officer to enforce the user maintenance schedule 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com SERVICE SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com CHAPTER I MAINTENANCE BY SERVICE PERSONNEL 1 1 CABINET AIRFLOW BALANCING After some period of usage the pressure drop across HEPA ULPA filters will increase otherwise known as filter loading By increasing the fan speed we can compensate this effect However there is a limit to this increment in speed which is the maximum supply voltage according to the fan performance curve If this happens and the required cabinet performance cannot be achieved the filters must be changed Please refer to the filter changing section below Please follow the steps described below to adjust the fan speed 1 Remove pre filter from
55. escoglobal com DEFECT REPORTING FORM To submit your warranty claim or to obtain a Return Authorization RA number please complete this defect reporting form and return it to Esco by fax or postal mail PRODUCT INFORMATION Model Number Serial Number Date of Purchase CUSTOMER INFORMATION Company Address Telephone Fax Email NAME OF ESCO DISTRIBUTOR Contact Company Country BRIEF DESCRIPTION OF ERROR WHEN DID THE ERROR OCCUR Transport Damage Unpacking Assembly Routine Operation RETURNING PRODUCTS PARTS Under Warranty For Service or Repair For Replacement Credit Note Requested Name Date Signature Defect Reporting Form 39 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com The use of non Esco parts and or parts not supplied directly by Esco or our authorized distributors including but not limited to maintenance parts spare parts replacement parts system components and or system accessories shall void all expressed or implied warranties REPLACEMENT PARTS LIST PARTS LIST FOR 2 1 2 FEET 220 240V 50HZ Item Item Code Description No Per Cabinet MECHANICAL REPLACEMENT PARTS 1 FILTER _ 610X610 10 22 2 X2 X66MM Eff gt 99 99 EQR SP FTR12 qp 425Pa 0 45m s 1 EQR EL BL 2 BLOWER D
56. ets have also improved on this feature and some have UV timers to allow the user to control the decontamination cycle for example to turn the lamp on for a few hours then shut it down instead of leaving it on the entire night in order to conserve lamp life these lamps have a rated lifespan after which effectiveness rapidly deteriorates However in the late 1970s and 1980s as the use of laminar flow and biological safety cabinets became increasingly prevalent manufacturers became increasingly aware of the detrimental effects of the UV lamp when used on these cabinets It is the purpose of this technical paper to discuss the negative effects of using UV lamps in modern laminar flow and biological safety Appendix UV Lamps in Laminar Flow and Biological Safety Cabinet 54 E 21 Changi South Street 1 Singapore 486777 PHONE 465 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com cabinets in the hope that this will educate users purchasers and anyone involved in laminar flow or biological safety cabinet technology to ensure better safety all for laboratory users 1 The use of the UV lamp in laminar flow and biological safety cabinets is explicitly discouraged in all major international standards and recommendations May we refer the reader to the following references NSF Standard 49 for Class Biohazard Safety Cabinetry UV lighting is not recommended in Class II laminar flow biosa
57. fety cabinetry If requested by the purchaser it shall be installed in such a manner that it does not reduce the required performance of the cabinet UV irradiation can cause erythema of skin and eye damage Section 5 25 2 b European Standard EN12469 2000 for Microbiological Safety Cabinets Ultraviolet UV radiation is not recommended for use in safety cabinets However if requested it should be installed in such a manner that it does not affect the airflow and containment performance of the cabinet Annex A Section 2 c Australian Standard AS1386 5 for Clean Workstations Laminar Flow Cabinets It is recommended that the use of UV lamps be avoided The need for UV lamps should first be established by the user Special safety precautions need to be in use for protection of personnel products and materials There is a potential for misuse of UV lamps with resulting injuries and deleterious effects of pharmaceutical products Section 5 12 d Australian Standard AS2252 1 for Class Biological Safety Cabinets Installation of UV lamps is not recommended Section 4 7 e Australian Standard AS2252 2 for Class Biological Safety Cabinets Installation of UV lamps is not recommended Section 4 7 f Australian Standard AS2567 for Laminar Flow Cytotoxic Drug Safety Cabinets Installation of UV lamps is not recommended Section 4 7 Appendix UV Lamps in Laminar Flow and Biological Safety Cabinet 55 EC
58. global com WEBSITE www escoglobal com 4 2 CABINET START UP PROCEDURE 1 Adjust the seating position so that the user s face is above the front opening Adjustable stool is recommended Please refer to section 4 5 about ergonomics for more info Turn on the fan Please leave the cabinet on for 3 minutes before start working in order to purge airborne contamination from the work area Prepare a written checklist of materials apparatus necessary for immediate usage and surface decontaminate them before loading them into the work zone This is to prevent overloading and minimize the number of arm movement that might disrupt the airflow e Never use the cabinet to store supplies or laboratory equipment Always surface decontaminate everything before inserting them into the work zone Load and arrange the materials apparatus to minimize the movement of contaminated items over clean items by segregating the contaminated items from the clean items A clean long sleeves laboratory coat may be used to protect personal clothing Wash hands thoroughly using germicidal soap Wear gloves for hand protection Gloves should be pulled over the knitted wrists of the gown rather than worn inside Thoroughly surface decontaminate the work surface and interior surface of the window using 7096 ethanol or other disinfectant depending on the user s requirement Do not use any disinfectant containing chlorine based substance as this will c
59. he strapping a Remove the strapping by cutting it at a safe position to prevent any scratch on the surface of your new cabinet wed Fig 6 Removing the strapping b Do not discard the packaging material for your cabinet until you have checked all of the components installed and tested the unit 7 Lifting the cabinet a The cabinet is not designed to support its own weight at the centre portion for extended periods However lifting the cabinet by mechanical lift truck in the centre is allowable If you must lift the cabinet manually use at least 4 persons b Always lift up the cabinet with enough distance in between the fork of the mechanical lift truck and the centre of the cabinet Suggested distance 50 cm for 3 ft and 90 cm for 4ft and above Install the cabinet on the existing work surface or Esco support stand retrofit kit Chapter Unpacking Your Cabinet 15 USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com e When installing the cabinet onto an existing work surface ensure that the structure can safely support the combined weight of the cabinet and any related equipment Some modifications to the work surface may be necessary work surface should be smooth and nonporous and resistant to the disinfectants and chemicals used in conjunction with the cabinet e Instructions on installing the Esc
60. hich is usually the case for reasons of convenience they cause unnecessary airflow turbulence which can in turn disrupt product and cross contamination protection In other words the UV lamp causes turbulence which disrupts the laminar unidirectional nature of the air stream which is critical to maintaining proper cabinet product cross contamination performance As detailed in many of the international standards above the cabinet is tested with the UV lamp in place in order to ensure that it does not degrade this performance aspect Again while Esco ensures this by conducting rigorous testing in our in house research laboratory this may not always be the case for other manufacturers In conclusion while it is reasonable to say that the UV lamp largely has no effect on the product protection and cross contamination protection of the cabinet it is also very reasonable to conclude that by eliminating this variable the overall performance of the cabinet can be increased especially when the other factors in this document as considered as a whole 4 Ineffectiveness of the UV lamp on modern laminar flow and biological safety cabinets When considered in the modern perspective of laminar flow and biological safety cabinet technology the ultraviolet lamp on the whole in generally ineffective for the following reasons a The laminar flow or biological safety cabinet should be operated 24 hours a day an economic possibility especially when
61. hich not only pose a safety risk but also makes the use of the lamp ridiculous In other words the user turns the lamp on exits the laboratory returns the next day and shuts the lamp down Even during the decontamination at night of the cabinet by the lamp the user did not realize that air in the room was already moving into the cabinet thus contaminating all exposed surfaces thus negating the use of the lamp c Presence of surface contamination in the work zone During the period when the blowers are shut down the front night covers installed and the UV lamp activated clearly there is ample opportunity for contaminated room air to infiltrate and contaminate the work zone UV lamps are ineffective as long as any surface contamination dust particles etc is present as UV has limited penetrating power d The lamp does not decontaminate all surfaces in the cabinet On some cabinet designs the placement and design of the lamp means that not all surfaces in the cabinet are exposed to UV radiation This is especially the case for biological safety cabinets where the lamp on all cabinet designs does not decontaminate the critical contaminated air return plenum beneath the work surface In addition occasionally some users disregarding manufacturer recommendations may store objects inside the cabinet When this is the case the UV lamp is totally ineffective for these shadowed areas e The lamp must be cleaned regularly to remove surf
62. ilters How Does a Laminar Flow Cabinet Work A laminar flow cabinet creates a particle free working environment by taking air through a filtration system and exhausting it across a work surface in a laminar or unidirectional air stream Commonly the filtration system comprises of a pre filter and a HEPA filter Because the air within the cabinet does not contain any airborne particles it is also sterile The laminar flow cabinet is usually enclosed on the sides and kept under constant positive pressure in order to prevent the infiltration of contaminated room air Laminar Flow Cabinets and Cleanroom Technology In order to further understand the technology behind laminar flow it is helpful to consider that these cabinets were first developed as an adjunct to cleanroom technology Just like the interior of a laminar flow cabinet cleanrooms are also controlled clean environments used in many modern industries However while industrial cleanrooms can span thousands of square metres with sophisticated protocols entry systems and working garments a laminar flow cabinet provides a much more economically viable solution for smaller scale processes The only drawback of the laminar flow cabinet is its inability to accommodate extremely large pieces of equipment due to physical constraints within the working zone What Are Laminar Flow Cabinets Used For In industrial applications laminar flow cabinets can be placed within a cleanroom t
63. ionth of a meter The medium of a typical HEPA filter is a single sheet of borosilicate fibers which has been treated with a wet strength water repellant binder The filter medium is pleated to increase the overall surface area and the filter is then attached to a metal frame Esco only uses the latest HEPA filters produced using the latest minipleat manufacturing process which lowers costs without compromising efficiency We are probably the only manufacturer of laminar flow cabinets to own our own filter production facility All HEPA ULPA filters used in Esco Laminar Flow Cabinets are of the highest quality and are laser scan tested after production to ensure leak free performance and efficiency After being mounted onto the each cabinet each filter is once again individually tested at our factory for filter leaks with the most sophisticated airborne particle counters Esco ULPA HEPA Filter Typical Penetration at 0 45m s 0 00035 0 0003 0 00025 6 0 0002 5 9 0 00015 0 0001 0 00005 0 0 0 05 0 1 0 15 0 2 0 25 0 3 0 35 Particle Size micro meter Appendix Introduction to Laminar Flow Cabinet 53 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com APPENDIX B UV LAMPS IN LAMINAR FLOW AND BIOLOGICAL SAFETY CABINET Introduction Ultraviolet light is part of the electromagnetic EM spectrum and
64. is divided into 3 wavelength ranges UV C from 100 nanometers nm to 280 nm UV B from 280 nm to 315 nm and UV A from 315 nm to 400 nm The germicidal ultraviolet lamp emits high intensity ultraviolet radiation concentrated around the wavelength of 253 7nm i e UV C radiation 9596 of the radiation emitted by germicidal lamps is at this wavelength which also happens to be in the region of maximum germicidal effectiveness Before laminar flow cabinets were developed in the 1960s and 19705 biologists in the laboratory used dead air boxes constructed of plastics with nothing more than an UV lamp in them to keep the interior of the box sterile The UV lamp would be turned on to decontaminate the interior of the box switched off leaving the box ready for use Of course we have come a long way since then the dead air box system was rudimentary and without any positive pressure airflow from inside the box to the outside contaminated room air could easily have been induced into the box again Naturally as the use of laminar flow cabinets become increasingly popular UV lamps were also incorporated on these new clean air devices for their germicidal properties Typically in a laminar flow or biological safety cabinet the UV lamp is activated while the cabinet is not in use to keep the interior of the work zone clean and decontaminated for example throughout the night until the user returns the next day to use the cabinet again Modern cabin
65. laying in terms of inches of water Inches W C Using the gauge on HEPA Filtered Clean Air Devices A HEPA filter when new and unused has a certain pressure drop to produce a certain amount of airflow For example new Esco filters are rated at less than 125Pa 0 5 W C to produce an linear air velocity across the filter of 0 45m s or 90fpm Most manufacturers try to optimize the performance of their HEPA filtered clean air device by minimizing this initial pressure loss The lower the initial pressure loss value the less work the fan has to do to maintain airflow and the more efficient the fan system However as a filter loads during the normal course of operation to maintain the same air velocity across the filter the pressure loss will increase For example the pressure drop required to maintain 0 45m s or 90fpm on an Esco filter may increase from 125Pa 0 5 W C to 150Pa as the filter loads This is due to the fact that during the normal course of operation the filter will Appendix The Role of the Pressure Gauge in HEPA Filtered Clean Air Devices 61 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com collect fine particulate matter in the media bed causing greater resistance to airflow In practical terms all manufacturers would recommend that the user adjust a variable speed control regularly to increase the
66. loor jack or mechanical lift truck has always entered fully under the crate in order to achieve stability Failure to do so will increase the risk of the crate falling off the floor jack or mechanical lift truck during handling Please use a suitable extension bar when the situation arises Fig 5 Moving the Crate Using Extension Bar Chapter II Unpacking Your Cabinet 13 J USER SECTION c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 3 Opening the crate a Use a hammer or crowbar to open the top panel carefully Be aware of exposed nails b Remove the front panel followed by the two side panels and finally the back panel c All the retrofit kits are packed separately and placed on top of the cabinet These items are listed on the packing checklist pasted on the crate Please check the packing checklist carefully to make sure that all items have been included Remove them from the top of the cabinet before proceeding further If you did not receive one or more retrofit kits listed on the packing checklist or if any of the items are damaged please contact your distributor or Esco Micro Pte Ltd immediately for further instructions Please refer to section 2 2 for complete listings of items aside from the retrofit kits included with your cabinet 4 Removing the packaging material a The cabinet is protected by styrofoam and shrink wrap
67. m before loading them into the work zone This is to prevent overloading and minimize the number of arm movements that might disturb the airflow e Never use the cabinet to store supplies or laboratory equipment QUICKSTART 0 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com 13 Arrange the materials apparatus to minimize the movement of contaminated items over clean items by segregating the contaminated items from the clean items 14 A clean long sleeves laboratory coat may be used to protect personal clothing 15 Wash hands thoroughly using germicidal soap Wear gloves for hand protection Gloves should be pulled over the knitted wrists of the gown rather than worn inside 16 Thoroughly surface decontaminate the work surface and interior surface of the window using 7096 ethanol or other disinfectant depending on the user s requirement Do not use any disinfectant containing chlorine based substance as this will cause corrosion to steel and stainless steel resulting in irreparable damage to the cabinet structure Do surface decontamination as well to the UV lights and electrical outlet when present 17 Minimize room activity personnel movements closing and opening of doors etc 18 Minimize arm movement Move arms in and out of the cabinet slowly to avoid disrupting cabinet airflow 19 It is recommended that the cabinet be operated contin
68. nt required are specified in details on the test report It is recommended that these tests be performed only by a qualified technician who is familiar with the methods and procedures for certifying laminar flow cabinets Esco conducts training courses on the validation and certification of laminar flow cabinets If you are interested you are most welcome to contact your distributor or Esco Micro Pte Ltd Chapter I Maintenance by Service Personnel 33 SERVICE SECTION REPLACEMENT FILTER AHCZLHC 1 REMOVE BACK COVER 2 UNSCREW NUT USING SPANNER 3 THE FILTER CLAMP 4 HEPA FILTER LEAK TESTING OF HEPA FILTERS LEAK IS FOUND IF GASKET LEAKS IF FILTER MEDIUM LEAKS TIGHTEN CLAMPS OR SEAL WITH SILICONE EXAMINE FILTER FOR DAMAGE 5 11 al SI 35VINVG ON dl SEAL WITH EXAMINE SILICONE VERIFY THAT FRAME FOR LEAK IS NOT BYPASS IF FRAME IF CABINET DEFECT IS CABINET LEAK RE EXAMINE FRAME FOUND LEAK IS FOUND SI INON dl ROTATE FILTER END FOR END 180 IF LOCATION OF LEAK IS UNCHANGED SI dl IF NOT A CABINET LEAK REPLACE FILTER AND RE TEST AFTER REPLACEMENT IN ACCORDANCE IF LEAK MOVES WITH FILTER WITH BS 5295 EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com C
69. o create an ultra clean mini environment When a laminar flow cabinet rated at Class 10 or Class 100 levels is placed within a Class 1000 cleanroom the air within the cabinet can often meet Class 1 levels Appendix A Introduction to Laminar Flow Cabinet 50 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com This arrangement is usually very economical versus operating a full sized Class 1 cleanroom However the most common application of the laminar flow cabinet is using it to provide an individual clean air environment for small items not requiring a full size cleanroom In the laboratory individual laminar flow cabinets are commonly used for specialized work such as tissue culture to eliminate airborne contamination would otherwise interfere with work processes Particles of 0 5 microns in size per cubic foot of air The lower the cleanliness class the cleaner the air For more information refer to the US Federal Standard 209E If air flows in straight parallel paths throughout an enclosed space the airflow is said to be laminar In Esco Laminar Flow Cabinets the laminar flow is a stream of clean air conditioned by a sophisticated industrial grade filtration system Proprietary construction technology guarantees that the internal plenum of the cabinet is pressurized uniformly This serves to increase filter life by evenly loading th
70. o support stand can be found inside the cardboard box with the support stand Esco Micro is committed to protecting the environment Please re use the pallet if possible and or dispose the packaging materials and crate in a proper manner Please hammer any exposed nail on the crate surface prior to disposal 2 2 PACKAGING CONTENTS The following items are included together with your manual 1 Test certificate 2 Test report In case this manual and or test report is lost or misplaced Esco retains a copy in our files A replacement copy can be obtained by contacting Esco Micro Pte Ltd and stating the cabinet model serial number and a brief description of the information desired 3 1 bag containing fasteners replacement 4 1 screwdriver for maintenance and service The following items are permanently attached to the cabinet in a Ziploc bag 1 Test report 2 Electrical diagram d Chapter Unpacking Your Cabinet 16 USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com CHAPTERIIII 3 1 CHOOSING A SUITABLE LOCATION Please follow these guidelines when choosing a suitable location for your cabinet 1 location must be far away from a personnel traffic flows b air vents in and out c door and window d any other sources of disruptive air currents or air drafts If drafts or other disruptive
71. osition throughout the day Don t sit in one fixed posture all day 5 Eyes some guidelines a Give your eyes frequent breaks Periodically look away from the work area and focus at a distant point b Keep your glasses clean 6 Arrange and position your frequently used work materials to minimize the possibility of straining yourself 7 Exercise regularly Esco provides some ergonomics accessories such as Lab chair b Footrest Please contact your local distributor or Esco for further information Chapter Operating Your Cabinet 29 AE USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com CHAPTER V MAINTAINING YOUR CABINET 5 1 MAINTENANCE SCHEDULE Please kindly follow the suggested maintenance schedule in order to maintain your Esco cabinet at its optimum performance Daily 1 Thoroughly surface decontaminate the work surface and interior surface of the window using 70 ethanol or other disinfectant depending on the user s requirement Do not use any disinfectant containing chlorine based substance as this will cause corrosion to steel and stainless steel resulting in irreparable damage to the cabinet structure Do surface decontamination as well to the UV lights and electrical outlet when present When cleaning the interior the operator should not insert any part of his her body into the work zone exce
72. over voltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its over voltage protection means For example in CAT Il which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient over voltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 7 Pollution Degree 2 0 Pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation m Chapter III Installing Your Cabinet 18 USER SECTION 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com t LFC 9 pO ex m Le De LFC LEGEND LFC Laminar Flow Cabinet Return Air Grille Air Inlet Personnal Traffic Fig 7 Examples of the influence on cabinet airflow from room design and ventilation Picture explanation No 1 The location of cabinet 1 is appropriate with respect to the avoidance of excessive air movements from the surrounding No 2 Cabinet 2 is too close to the doorway and could be influenced by the
73. pt hands Monthly 1 Using a damp cloth clean the exterior surfaces of the cabinet particularly the front and top of the cabinet to remove any accumulated dust 2 Check all the service fixtures when present for proper operation 3 All daily activities Quarterly 1 Replace pre filters 2 All monthly activities Annually 1 Have the cabinet recertified by a qualified certification technician Please refer to performance validation certification section on Chapter 3 section 3 4 2 Replace the UV lamp when present 3 All quarterly activities 8 Chapter V Maintaining Your Cabinet 30 j USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com Biannually 1 Replace the fluorescent lamps 2 All annual activities 5 2 DISINFECTING AGENTS 1 For stainless steel all common disinfecting agents except chlorine based agents are suitable 2 For powder coated surfaces all common disinfecting agents are suitable However the cabinet has been specifically evaluated for use with the following 1N hydrochloric acid 1N sodium hydroxide 196 quaternary ammonium compound 596 formaldehyde 5 000 ppm hypochlorite 2 iodophor 5 phenol 70 ethyl alcohol 8 Chapter V Maintaining Your Cabinet 31 j USER SECTION User Monthly Maintenance Schedule Model Year Serial No Op
74. ptional accessories to be easily fitted later in the field Please read the manual to take full advantages of the above features and familiarize yourself with proper installation operation and maintenance of the cabinet We recommend that this manual together with the factory test report be kept near the cabinet for convenient reference by operators and qualified maintenance personnel If you have any questions that are not addressed in this manual please do not hesitate to reach us by e mail at biotech Qescoglobal com or give us a call at 65 65420833 With warm regards from Singapore 7 Lim Lay Yew Chief Executive Officer Esco Micro Pte 21 Changi South Street 1 Singapore 486777 65 65420833 FAX 65 65426920 Visit our website at www escoglobal com EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com TABLE OF CONTENTS Table Of 1 Warning sam 3 Declaration of 4 2 2 4 Freight Claim 5 Warranty Terms and 5 7 Corporate Profile Biotechnology Equipment Division 8 22 A USER SECTION ue I Basic Product
75. result in the carrier refusing to honour a claim Contact delivering terminal to arrange for a claim form and inspection report to be faxed or mailed to you e Notify Esco Micro Pte Ltd regarding which items need replacement Keep all damaged items and packing material until claim is resolved between you and the carrier ACCEPTED FREIGHT WITH CONCEALED LOSS OR DAMAGE When a damage or loss is discovered during unpacking e Contact the carrier immediately upon discovery of damage and request for inspection by the carrier s agent Carrier will determine inspection needs based on value and time elapsed Freight Claim Information 5 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com Notify Esco regarding which items require replacement Esco will fax to you an order acknowledgment that includes value of items for claim less freight amount Esco will invoice you for replacement materials Esco standard payment terms will apply UNACCEPTED FREIGHT If substantial damage is noted upon inspection you have the right to refuse part or all of a shipment Do not unpack pallets or crates with damaged materials Individual items cannot be refused You must refuse the entire pallet or accept the freight with noted damage see above Esco will handle all freight claim procedures and process a replacement order for your company for the damag
76. speed of the fan system above its initial speed setting to compensate for this increase in resistance and maintain airflow in the system This adjustment must in most cases be validated by a true airflow velocity meter Adjustments CANNOT be performed using the pressure gauge This is because the pressure drop across the filter to maintain the SAME amount of airflow is NOT a constant and depends on the health of the filter It is only a known constant when the filter is new In any HEPA filtered clean air device the user s concern is undoubtedly to maintain a proper quantity of airflow in the system On a laminar flow cabinet the user s goal will be to maintain a laminar airflow velocity of approximately 0 45m s or 90fpm for example On a Class biosafety cabinet the user will try to maintain certain downflow and inflow setpoints velocities which in turn translate to a certain amount of airflow across the entire filtration system Airflow NOT pressure loss across the filters is the primary factor that translates into performance and or safety apart naturally form other aspects such as filter integrity and efficiency The pressure loss across the filter as indicated by the differential pressure gauge may be thought of as an indicator of the health of the filter or the efficiency of the entire blower system provided that airflow is maintained by a speed control adjustment and b validation with a true airflow velocity meter The higher
77. ss the filter into a region that has a pressure roughly equal to that of the ambient atmosphere This last region may be for example the work zone of a laminar flow cabinet In theory this region may be defined to be under slight positive pressure due to velocity pressure or the pressure exerted by the laminar air stream but in practice it may be considered to have a pressure equivalent to that of the ambient environment In this scenario the fan can be thought of as blowing air across the filter It is also possible for a fan to be sucking air across a filter fundamentally this is the same and a pressure difference also exists The pressure difference is also referred to a pressure loss across the filter or a pressure drop across the filter Therefore a pressure gauge indicates the difference in pressure between the plenums present before and after the filter In the example above blowing the pressure gauge indicates the Appendix The Role of the Pressure Gauge in HEPA Filtered Clean Air Devices 60 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com difference between the region of high pressure before the filter and that of the ambient environment atmosphere Practically this value is derived by subtracting the ambient pressure value from the actual value of the positive pressure plenum before the filter Note that on all HE
78. th more than 20 of the world s most recognized standards of local regional and international scopes In particular testing in our laboratory is most frequently conducted based on EN12469 NSF49 and IEST RP Chapter Basic Product Information 12 a USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com CHAPTER III UNPACKING YOUR CABINET This chapter aims to provide relevant information on how to handle the cabinet properly upon receipt Failure to follow the following instructions may damage the cabinet We strongly advise you to read this chapter carefully before proceeding further Relevant information on warranty terms and conditions can be found on page 7 2 1 STEP BY STEP PROCEDURE 1 Inspecting the crate Upon receipt of your new cabinet inspect the crate If there is any visible damage to the exterior of the crate please refer to freight claim information on page 5 NB Some cabinets may be packed with corrugated carton instead of a wood crate in which some of the following instructions would not apply 2 Moving the crate a The crate is designed to protect our cabinet from any foreseeable circumstances However excessive impact onto the crate may also damage the cabinet Prevent any direct impact or hitting to the crate when moving b When lifting the crate please always ensure that the f
79. the operating pressure loss of the filter the poorer its health and the less efficient the blower system The user will note that on every filtration system there are two operating pressure points that can be determined When the filter is new there will be a certain operating point to maintain a certain airflow setpoint As the filters become loaded and the speed of the fan is increased to maintain airflow the filtration and fan system will soon reach another operating value this operating value can be thought of as a maximum beyond which the fan cannot work any harder to deliver airflow Hence a theoretical application of the pressure gauge is provided that airflow is maintained and measured with an independent meter as a liner scale of filter loading a scale ranging from the initial value explained above to the final value That is as the reading on the gauge increases from the initial value the user will know how much pressure capacity the fan has left to maintain airflow As the reading on the gauge approaches the known maximum the user will know that the filter must soon be replaced In practice this is hard to apply because for every single unit the initial and final operating points may be different Appendix The Role of the Pressure Gauge in HEPA Filtered Clean Air Devices 62 EC E 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal
80. the top of cabinet 2 Adjust the speed control to achieve the required velocity 3 Recertify the cabinet when speed is adjusted 1 2 FILTER CHANGING The HEPA ULPA filter under normal usage and barring an accident a puncture do not need replacement until the airflow velocity cannot be maintained at the specifications required by the test report even though the fan has been set to maximum speed The filter changing procedure is described in detail at the end of this chapter The cabinet must undergo recertification after filter changing Before the new filters are installed all surfaces should be thoroughly cleaned of silicon and or adherent gasket material The new filter should be carefully handled and examined prior to fitting It is important that the filters and the gaskets be checked for leaks prior to use Chapter I Maintenance by Service Personnel 32 SERVICE SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com 1 3 RECERTIFICATION The following tests should be performed 1 Airflow velocity 2 Particle count test 3 Filter leak test filter media leak test and filter gasket leak test Please refer to the flow chart at the end of this section 4 Site installation assessment test 5 Optional tests a Electrical safety b Light intensity test c Noise level test d UV intensity test The testing methods and equipme
81. tors are welcome to visit the Esco Biotech Equipment Technology Centre located in Singapore at our worldwide headquarters which features a demonstration laboratory conference training facilities for visitors and various testing laboratories in which state of the art research and tests are continuously conducted into improving the performance of our products Fig 3 Wind Tunnel Test Facility Corporate Profile Biotechnology Equipment Division 9 EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com USER SECTION EC c 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 465 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com CHAPTER I BASIC PRODUCT INFORMATION Horizontal laminar flow cabinet offers proven protection for your sample and processes where operator protection is not required They are suitable for application which requires ISO Class 3 air cleanliness within work zone as per ISO 14644 1 equivalent to Class 1 per US Federal Standard 209E AIRFLOW PATTERN INSIDE THE CABINET Room air is taken in from the top of the cabinet through a disposable pre filter with 8596 arrestance this serves to trap larger particles and increase the life of the main filter Air is forced evenly across an 14 HEPA ULPA filtered vertical laminar flow air stream within the cabinet the result is a stream of clean laminar air
82. uously whenever possible to ensure cleanliness 20 Wash hands thoroughly using germicidal soap Turn off the cabinet 21 Maintain your cabinet please refer to chapter 5 for more details QUICKSTART 06 AIRSTREAM HORIZONTAL LAMINAR FLOW CABINETS USER amp SERVICE MANUAL ECCO Esco Manual Revision No AHC v1 07 2004 Specifications subject to change without notice WELCOME NOTE FROM Congratulations You have just purchased one of the finest laminar flow cabinets available It is a pleasure to welcome you to the growing number of customers who own and operate Esco laminar flow cabinets For your convenience all exposed exterior and interior surfaces of this products has been and wiped down in a controlled clean environment protective coverings on stainless steel and plastic surfaces where present have also been removed Your new Esco Airstream Horizontal cabinet includes many unique features and exciting improvements to simplify maintenance enhance safety and improve ergonomics These include e transparent UV resistant tempered glass sides to increase operator comfort and protection 304 grade stainless steel work top for greater cleanability work zone lighting level above 1200 lux 111 foot candles using 5000k fluorescent lamps for greater comfort and visibility during use e integral metal filter guard on the ULPA filter prevents accidental damage to the filter media retrofit system that allows o
83. w escoglobal com Alternatively the user can also complete the warranty registration form which can be found within this manual on page 38 Please take note that when returning the defective product or part s you must first obtain a return authorization RA number from Esco and it must be noted on the documents accompanying the return The defect reporting form can be found on page 39 Warranty Terms and Conditions 7 EC e 0 21 Changi South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 E MAIL biotech escoglobal com WEBSITE www escoglobal com CORPORATE PROFILE BIOTECHNOLOGY EQUIPMENT DIVISION Fig 1 Esco Headquarters in Singapore Esco supplies many high tech industries with critical equipment that help keep products people and processes free from contamination For example our cleanroom construction components help pharmaceutical manufacturers meet GMP standards keeping the drugs they produce clean Our laminar flow mini environments help microelectronics manufacturers increase product reliability and yields In the laboratory researchers use our safety cabinets for clinical biological research in order to develop vaccines for dangerous diseases In a small way Esco contributes towards these essential technologies that have become the backbone of our modern lives The Esco Biotechnology Equipment Division is a highly focused manufacturer of laminar flow biohazard safety and other HEPA filtered cabinets for the l
84. within the work zone of the cabinet this dilutes and flushes all airborne contaminants from the interior A nominal filter face velocity of 0 45 m s or 90fpm ensures that there is a sufficient number of air changes within the enclosed are of the cabinet in order to maintain cleanliness The purified air travels across the internal work zone of the cabinet in a horizontal unidirectional stream and leaves the main work chamber across the entire open front of the Room Air cabinet Negative Pressure Plenum Filtered Laminar Air Flow Fig 4 Airflow Pattern Inside the Cabinet O Chapter Basic Product Information 10 USER SECTION 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com SAFETY Raised edge at the back of work zone prevents spills from damaging the filter e Integral filter metal guard on the ULPA filter prevents accidental damage to the filter media Seamless filter gasket is permanently moulded on the filter frame and will not deteriorate over time e UV resistant tempered glass sides provide a high level of protection against harmful UV rays when a UV lamp is installed Fulfil the safety requirement of IEC 61010 1 EN 61010 1 UL 3101 1 CSA C22 2 No 1010 1 92 All components are UL listed recognized PERFORMANCE e Intelligent blower system which automatically compensates to
85. zimooe v9 vo 85 21 90 v9 VOL 85 1 90 v9 vol ONILVY 1NOO dds v GOW asoyd oibus ZH 09 OvAOEL OLL Alddns 19 ATA I 4034 ajgjsuodsaa puo 4284402 50 SuoOlL02 jD2 ds puo aux Aqauay n isvtiva e za A ddng 4 13 6 8 8 an Lleyn 22723 kis vi jg lt gt wea MS 1461 ss ise 21 8 ns Wig 318 lt e jus pde 390 wed ava JeMo g YN MS 5 NNUS y MY y nee eee bz NIV div 28005 29 ws YONO s CR 310N DS 3 N 21 South Street 1 Singapore 486777 PHONE 65 65420833 FAX 65 65426920 e E MAIL biotech escoglobal com WEBSITE www escoglobal com ECC CHAPTER Ill TROUBLESHOOTING OF COMMON PROBLEMS Problem Possible Causes Corrective Action Cabinet does Wrong electrical Check whether electrical cord has been connected not start connection properly Power failure 1 Check for electrical power at the wall outlet 2 Resetthe circuit breaker by pressing the

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