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1. Error Information Meanings System Error 03 Error of horizontal movement organ System Error 04 Error of vertical movement organ System Error 08 Failed to write in Flash ROM System Error 09 Error with system ROM System Error 10 Error with system RAM NOTE e Ifyou find any problem that you can t solve by yourself or a problem repeats please contact the supplier for help e Different version of instrument will be different in configuration these changes will not affect the capability 3 2600C user s Manual Trouble 1 When switch on the machine there is no display and the machine does not Start up 2 Do not suck up the liquor and the vacuum pump does not work 3 Do not dispense the liquor 4 Liquor is out of control and dispensed all the time 5 The system always gives out warning of waste bottle full Shooting Check the electric supply socket and fuse Check the power supply to the control board for 5v 12v and 24v input Check the power supply for 24v output Check the dispensing head for jam and the electromagnetic valve for malfunctions Check the electromagnetic valve for malfunctions The waste bottle 1s full just screwing out the cap pours out the waste liquid and screw down the cap again The sensor cable has not been connected to the connecter at the rear of the washer 32 2600C user s Manual Appendix I Technological Parameters Wash
2. avoid unnecessary personal injury 2600C user s Manual Precautions General Before you start installing and working with the washer you should read the safety precautions and regulations shown in this chapter Operator Qualification Please note that the operation with washer should be carried out only by the doctor or clinical inspector who have undergone necessary training provided by the sales agent Service Technician Qualification install maintain and repair the instrument a service technician has to be trained on the instrument by the manufacturer or their representative A service technician is also expected to be familiar with the normal operation of the instrument as described in the User s manual and the special operations as described in the service manual ATTENTION Cautions Electrical To use washer safely pay attention to the following items TO prevent the risk of electrical shock and or damage to the instrument Operator should not open the cover of the instrument Only authorized personnel for example service technicians may open the instrument to perform maintenance or repair Touching the main board when the power is on may cause severe injury or death Any problem please ask for helps from your supplier Mechanical Pin tips are sharp and may cause bodily injury Do not place hands or fingers under the pins while the instrument is in operation Always set the power switch to OFF befo
3. Locate 2600C to avoid exposure to excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations Leave sufficient clearance 10 cm at both sides of the unit for adequate air circulation ATTENTION Cautions Instrument be operated within an ambient temperature range of 10c 40c humidity of 20 85 2 3 Power requirements e AC200V AC240V e 50 60Hz e 150W ATTENTION Cautions e Ac power must be grounded at the main socket Interruption of the protective conductor inside or outside the instrument or disconnection of the protective conductor terminal may make the instrument dangerous e The circuit used should be substantially free of large transients such as large pumps large centrifuges Etc e If found smog strange sound in instrument please turns instrument off immediately and contact your dealer e Use only the fuse type and rating specified for this product 11 2600C user s Manual Connect the supplied power cable to the rear of the instrument as shown Plug the other end of the power cable into an AC outlet that has a protective conductor also called as earth or ground 2 4 Installing the Wash Bottle and waste bottle This dispense pump must be located inside the Wash Bottle and the dispense tube and dispense pump power cable must be connected To install the Wash Bottle 1 Plug the pump connector into the soft tube emphatically As indicated in th
4. 5 9 SPecily the MiCrOplale RM HT A 16 3 92 specily the positionor the wash head uoa et C vet sr ere es Cerdo dte doe eee 16 3 3 5 Specify the speed of diving pump eesse Eaa nennen nennen nnn rrr nnns LT 3 3 6 specify the Top Aspiration position ssssssssssseeeeeeeeeee nennen nnne nennen nnn n n nnn nnns 18 AOA ex s d 10 CO ne ne Oe aM ECHTE 19 Chapter 4 Operation OBTBSCRUEDeDIE o eo isi ted e eate rn e Etna t a a a E 20 di Work area and Mi Crop late 2 oct d corti apo dovezoxan Pagan ettet dtd vi OO d dub oa Edda NDA ES 20 2 2 Mas prOCOdUT Oeo eme IE Dar eeu bue o eM o dtd A accu edutsa Ged AEN Len ea d iE eae 21 2 PU scoop Sans pA sone ese oat eet ne os baie A M LM MI EU 22 ZO TRANS MN AEE O eras E T NC Ado on Gece au nome Ro E AEE E E E E UAE neste 23 Adis pense OISD seecbeebivadiect uae onte npa voL E uec uas evan Uie ta usta D aad OD SEE 23 A GA Siitate C ASDE aebussin bed asocio dan E oa ibas tena dese ceu de Levi bocud dai bossed estat 24 Chapter x Daily NIaimiteatiCe seo venie ie Eee RUE Rr ognuna ud a eae Vae eM cx ERR UM 27 2 I3mathitenatice o WOLK dfe io a Tele ret heutu ie pt esten ume tulu Go edie wa db eU uiu US CR DeR UU 24 25 2 myatitepiatice OF Was edtbenssd a n pEE o S E oes DE bate ideo nesaa pbi s AMD UM 2f 2 2 Disposal oF waste DOM G 2 259 ens UbietadiPta duae pe iiec Cote piu pM ED Pi cR EI DLE ALME 28 S ialhtelabice oL UDG dae cicess cherie chien oxide x bites Es vim Vut daly assa
5. adjusting resolution is 50 L When the dispense volume is more than 350 L the software of the washer will judge automatically and do the top aspiration while dispensing Soak time The time between dispensing and aspirating wash liquid At the row mode the time is 1s 60s and at the plate mode the time is 1 seconds to 8 hours At the waiting screen click start to enter program menu No 1 Wash Mode Plate Wash Times 1 Aspiration Single Fluid Volume 350 L Soak Time 0h00mo0s Figure4 2 Program Click SELECT to select each parameter Press Lth m to change its value After set all the parameters click SELECT to the next step 21 2600C user s Manual Strip setting Figure4 3 Strip setting Click CANCEL to back to the waiting screen click START to start washing Figure4 4 Running of program Click CANCEL to stop during washing procedure Click again the wash head will back to the waiting groove after that the system will back to the waiting screen When the waste bottle is full the system will give out a warning and the screen displays like this Waste bottle full Figure4 5 Waste bottle full 4 3 Prime Prime means to fill the pipeline of the washer with wash liquid to make its concentration meet the requirement Prime is required in the following situation 1 Newly installed instrument 2 Restart to use leave unused instrument 3 After rinsing procedure 4 After changing supplyin
6. bau rove RAN tu eus lo bbs vts utque 28 Chapter o Replacement OE Spare Pat boue dt e a EDDIE Udo papse dad eminredeborunmiEre D oU du ond 29 Gil Replacement Ol Wasi ead scire pev ane veteris eat atan itodus fasi has Gat ur iusted Le Lp ehe 20 6 2 Replacement of power supply iiio he ero A edad Led e e E RENI tessa ei 29 6 3 Replacement of Dispense PUMp 0c cccc cece eee e eee e eee e eee senem ens 29 64 Replacement or Sold Valve ienei an aa e OEA 30 Chapter 7 Trouble ShOOun aises a a a ue eas RIE 3 Appendix I Technological Parameters 2600C user s Manual 2600C user s Manual How to use the manual General Welcome to be the user of 2600C This manual is a user s guide for 2600C Medical Elisa Washer which includes system installation operating procedures parameters setting and basic maintenance Reading the manual carefully before operation is strongly recommended Instruments with different software versions will have some different functions this manual describes the 2600C Washer system and its software version If you have any problem please contact your supplier for help Please keep all the original package for the future use of transportation store and back to factory Symbols description Symbols in the manual NOTE Notes contain additional information or tips when using the instrument ATTENTION Cautions Cautions should be followed carefully to ensure your instrument work correctly and to
7. fluid volume and soak time discretionarily You can set wash by row or plate also you can skip some rows 5 Full automatic washing procedure Auto orientation Uses can do manual adjustment to ensure precision 6 Long and short pin designed for wash head which to reduce cross pollution 7 The waste bottle can auto detect the liquid and give out warning when it is full 8 Three wash bottle channel easy to switch 9 You can select 8 or 12 channel wash head 10 When connected with 2100C reader can perform long distance diagnosis and upgrade 1 3 Washer Principle z E ees solid valve WASH WASTE a dispense pump m manifold COLETTE a f aY vacuum exhaust vacuum pump liquid sm Jas Figure 1 1 washer principle Wash bottle solid valve dispense pump wash head compose the dispense tube path of 2600C First the wash solution is pump from the wash bottle the solution was dispense to the cuvette by short pins and then the wash liquid is aspirate from the cuvette by the long pins at the end the waste liquid was pumped into the waste bottle by the vacuum pump The dispense pump outside is used for sucking the wash solution and the solid valve the control of wash solution for cuvettes The vacuum pump suck the air in the waste bottle then vacuum appear from the waste bottle to the long pins of the wash head and then waste liquid is aspirated 1 4 Hardware Components Hardware components of the 2600C
8. head Spin or 12pin Liquid resolution 10uL Liquid volume control SOuL 3mL 8 well mode 50uL 12mL 12 well mode Wash mode Plate or Row Aspirate mode Single or Double Precision CV value lt 2 Remain volume lt 3uL Max dispense volume 2000uLx12 or 3000uLx8 Time to wash a whole plate Single cycle lt 105 seconds ab ee lt 250 seconds Max soak time Plate 24h59m59s Row 24h59m59s No of user define program 50 Volume of bottles Both wash and waste bottle is 2000ml n couplant of liquid Glass nylon Teflon polypropylene stainless steel Tygon pipeline Cubage LxWxH mm 410x343x178 Weight 9kg Power supply 220V AC 10 50 60Hz power consumption Max 150GA Using temperature 10 C 40 C Store temperature 1 C 40 C MRC Rev 01 11 09 33
9. one kind of microplate for a long time we set the selection of microplate as one item of the system settings instead of a parameter of user define program If you want to wash microplate different from the already set kind you should change the system setting of the washer The default set of microplate is flat ATTENTION Cautions If the microplate used for washing is not the same as the set in the system settings there will be a risk of damage to the wash head Changing the setting of the microplate will not affect the running of the original user defined program The software will judge automatically For example you set u or v bottom in the system settings but in the program you set double aspirate The washer will only do single aspirate Please follow the below steps to change the microplate settings At the waiting screen double click CANCEL to enter the system setting menu Click to choose specify microplate and click START to enter the submenu Changing Plate Flat Bottom Figure 3 4 changing plate Click H m to select kind of microplates Click START to save the change will be saved into the flashrom of the washer automatically 3 3 4 specify the position of the wash head As the shape and size of the microplates on market is various from different supplier The 2600C washer provides the user with the function of adjusting the position of the wash head You can adjust the position of the wash head o
10. to change the value its various range is 10 10 This is to micro adjust the voltage of the diving pump so as to adjust the inject speed of the wash liquid The recommend value is 0 17 2600C user s Manual Adjusting pump 0000 Figure 3 7 Adjusting pump 3 3 6 specify the Top Aspiration position When the dispense volume is more than 350 L the system will do Top Aspiration automatically to prevent overflow The Top Aspr setting allows change to Top Aspiration position you can adjust the Top Aspiration height during 2mm 2mm Click START to save and Cancel to ignore change Adjusting Top Aspr Figure 3 8 Adjusting Top Aspr Please watch the position of the wash head while you are adjusting the Top Aspiration position Make sure that the wash head has been adjusted to suitable position as shown in Figure 3 9 t li Figure 3 9 Adjusting Top Aspr 18 2600C user s Manual NOTE The Quality of washing often affects the validity of test results To b assure adequate washing perform periodic dispense volume repeatability checks as described in this manual rinse the pins after use handle and store the pins carefully to prevent damage use the prime cycle before each wash watch the instrument to see that each dispense pins is functioning properly ATTENTION Cautions Do not operate the instrument if the pressure is unstable or if any pin is damaged 3 2 Power Off On the main screen
11. CD s contrast When the contrast deviate too much from the best value maybe you can not see the menu clearly At this situation just following the steps below to operate without seeing 1 Turn on the instrument 2 Wait for the washer to complete its initialization 3 double click CANCEL twice quickly 4 Press START 5 Press HF o adjust the contrast until you can see the display clearly 6 Press START to complete settings 3 3 2 Specify the Wash head 1 Turn off the washer 2 Take out the wash head from the bracket disconnect the tubes and place the wash head in the package box for it 3 Take out another wash head from its package connect the tubes and place it well in the bracket Make sure that the wash head is parallel with the bracket 4 Turn on the instrument waiting for the prime procedure end and make sure that there is no leakage with the tubes and connectors 5 Select Changing Wash Head It allows to change the 12 pin or 8 pin plate change plate type Click START to save and Cancel to ignore change Changing Wash Head Figure 3 3 changing wash head ATTENTION Cautions Step 5 is required or it will cause wrong dispense volume The wash liquid will spray on the work area It will even cause damage to the wash head 2600C user s Manual 3 3 3 Specify the microplate The 2600C washer supports flat u and v bottom microplates As most users will use
12. Laboratory Equipment Manufacturer A www mrclab com C iz V3Net S THE STANDARDS INSTITUTION OF ISRAEL INSTRUCTION MANUAL FOR UT 2600C Medical ELISA Washer PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3 Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC VER 02 12 10 2600C user s Manual Table of Contents How 16 USC NS Tanui TT a 4 PC ALLO I See essen detto ad sc eh t echt Le 5 C see EET O ein ODD a EM nt ore Mra ert M aM 7 BEEd LUSG rr 7 V VDSS EM Oi cts E E S fi LW asm Principle senei T 1 DE Sire IEEE Compe NE D E T P 8 INE do decli EE 10 CIDDICEZE DS to Ne a O 1 6 oc E ee toto Moe DD MID NIE EID Te ILI MIN SUD DLL EE 1 DM NSO e RE EN 11 2 2 Bitvironthental Require ER DIES 3 525 25ssshansasanie taie mat ea ALL bac pute N 11 2D POWER regure men S aiscccotie Cod oa ee Pr co adem Eo voe bct toi olia pem sen ela ode bci uL clio intere o oM Poe dA 11 2 4 Installing the Wash Bottle and waste bottle 12 2 Installtag the wash Tie dd sus dateien pet eto deeds da uet ho b peut rato E 13 PA oW BUENO ETE 13 Chapter 3 Settings and Adjustment of Instrument eeeeeeiiiisesssessseeeeee nennen nennen nennen enses n nns 14 Pes 3 ee 1B eA Speer edd UE MM MELLE M MEE Door ene aM d 14 3 94 ANGUS ING COMMAS eee halen d cesses nate etit Gea ia eta rese tet ebat Aine a neiges 14 2 9 2 5 Deci the WV ash Die dd oio st pee RM essem A 15 9
13. acuum line and the external computer are shown at the rear of the panel 2600C user s Manual Power Switch Waste Bottle R5232 Interface Tube Connector Power Cord Connector Waste Bottle level sensor Dispense Pump it ish B ttle Vacuum Exhaust Power Connector Tube Connector Figure 1 2 Rear View of the 2600C system 1 5 Key Pad Description PRIME Start Prime procedure RINSE Start Rinse procedure DISP Start Dispensing procedure ASPR Start Aspiration procedure SELECT Change edit item Start wash procedure START Go to next operation Cancel current operation CANCEL Double click to enter system setting Change parameter Change parameter Figurel 3 Keypad 10 2600C user s Manual Chapter 2 Installation 2 1 Unpacking e Carefully unpack the instrument and remove it from the plastic bag e Place 2600C on a flat working surface e Place the wash bottle and waster bottle behind 2600C e Remove the power cable and other items from the packing carton e Check the components User s Manual Analysis Certificate packing list Accessories power supply cable fuse tube etc NOTE e Report any visible damage to your shipper or freight carrier at once e Retain the original packing material for future use in the event that the instrument is placed in storage shipped to another location or returned for repair 2 2 Environmental Requirements
14. are shown in Figure 1 2600C user s Manual EevyF ad Display Panel Dispense TubeAspiration Tube H Wash Head Handle rum Area Wash Head METRE _ Figure 1 1 The 2600C systems Wash head The wash head contains a set 1 x 8 or 1 x 12 of dual Wash pins Each set of wash pins includes a dispense pin and an aspirate pin closely spaced so that fluid can be aspirated from a well while fluid is dispensed into the same well The wash head moves back and forth to wash each row or column depending on the wash head used of the plate by lowering the wash pins into the corresponding wells Display Panel The system communicates with the user by display All system information and wash parameters entered by the user are displayed Keypad The keypad is used by the operator to select commands and input wash parameters RS232 Interface The system includes an RS232 interface so that an external computer can be connected for technical service purposes in the future the RS232 interface may be used for updating new version software Wash Bottle The wash bottle contains the wash fluid used for Dispensing Filling and Purging with a dispense pump inside Waste Bottle The waster bottle contains the fluid aspirated from the wells with a level sensor inside Connectors at the rear of the 2600C system are shown in Figure 2 Connections for the power cord the dispense pump power connector the waste bottle level sensor the waste bottle v
15. ble the frame of the power supply 3 Disassemble the power supply board and replace it with a new one Frame fixes the power supply _ K A EN 4 Figure 5 1 Power Supply 6 3 Replacement of Dispense Pump 1 Take out the dispense pump from the wash bottle pull the connector of tube out 2 Replace the pump with a new one and then connect the tube 3 Put the pump back to the wash bottle mm j 3 Connector of Pump Dispense Pump Figure 5 2 Dispense Pump 29 2600C user s Manual 6 4 Replacement of Solid Valve 1 Power off the instrument disconnect the power cable and open the upper cover 2 Snip off the small clip which fix the wires of power supply 3 Remove the four screws which fix the solid valve 4 Use a forceps to take off the two wires 1 amp 2 and then solder them with the two wires of new solid valve 5 Connect the two tubes to connectors of the solid valve and then fix with clips 6 Install the new solid valve the base of the instrument 1 blue 2 black 3 black 4 red Figure 5 3 the solid valve 30 2600C user s Manual Chapter 7 Trouble Shooting The 2600C washer will stop all the movement of mechanical parts when system error detected It will give out warning and display the error information on the screen When you find the errors Please contact the professional personal of the supplier Here are the error information and their meanings
16. cedure the system will stop all the movement of parts and displays following screen 25 2600C user s Manual User abort Figure 4 15 User abort Click any key to back to waiting menu 26 2600C user s Manual Chapter 5 Daily Maintenance 5 1 maintenance of work area 1 Wipe off the remnant liquid on the work area each time 2 Use tweezers to nip a small piece of absorbent cotton to wipe the work area 3 Please pay attention that the head of the tweezers don t scratch the surface of the worktable ATTENTION Cautions e Don t use any organic solvent oil and fat caustic liquid to clean the work area e Be careful to prevent the tweezers lacerating the surface of work area e Please wear gloves while doing the maintenance and wash your hands each time after work 5 2 maintenance of wash head The wash head is one of the core parts of the washer its state will affect the veracity of the dispense volume and the liquid remain volume So it is very important of the maintenance of wash head The suspended matter in the wash liquid will jam the pipeline Please use fresh wash liquid and usually check if there is suspended mater Remember to run rinse procedure after working with the instrument When you find the dispense volume of one row is obviously less than other rows it means that the corresponding dispense pin thin and short is jammed When you find the liquid remain volume of one row is obviously mor
17. d when using 8pin wash head the maximum number is 12 Click H Ht to change the value and click START to start dispensing The washer system also provides you with the function of setting by each row Click E m to select By each row and click START to enter the setting menu Figure4 10 Dispense by strip The sketch map of the microplate is displaying in the middle of the screen The black in the well means liquid Black means this row will be dispensed or it will not The arrowhead on microplate shows that the row is being set now Click m ck to switch each row and click to set dispense or not After setting click start to dispensing When it ends the system will back to waiting screen During dispensing click CANCEL to stop the procedure the system will stop all the movement of parts and displays following screen User abort Figure4 11 User abort Click any key to back to waiting screen 4 6 Aspirate Aspr Click to start setting the screen displays 24 2600C user s Manual Aspiration Single Figure4 12 Aspiration Setting ck h H to change parameters press CANCEL to exit and back to waiting screen click to next step 12 Figure 4 13 Strip setting Click CANCEL exit and back to waiting screen click to start dispensing Figure 4 14 Running Aspiration After the aspiration procedure the system will back to waiting screen automatically During aspirating you can click CANCEL to stop the pro
18. e following photo Figure 2 1 installs the pump 2 Fill the Wash Bottle with the wash solution 3 Place the dispense pump inside of the Wash Bottle and secure the cap as indicated in figure 2 2 Figure 2 2 Place the pump into the wash bottle 4 Connect the end of the wash tube to the Wash Bottle Tube connector at the rear of the instrument Figure 1 2 5 Connect the dispense pump power cable to the dispense pump power connector at the rear of the instrument Figure 1 2 12 2600C user s Manual 6 Connect the two tubes on the waste bottle cap to the waste bottle tube connector at the rear of the instrument Figure 1 2 7 Connect the sensor connector on the waste bottle cap to the waste bottle level sensor connector at the rear of the instrument Figure 1 2 NOTE Because the instrument is working with air pressure the mouth of the waste bottle and all the tubes b must be airproofed 2 5 Installing the wash head 1 Connect the dispense tube white connector and aspirate tube transparent connector with the relative connectors of the wash head and instrument 2 Place the wash head in the bracket ensure the two steel locating pins are in the two grooves 3 Adjust the plastic screw to parallel wash head with work area ATTENTION Cautions Pin tips are sharp and may cause body injury Be careful when you are installing the wash head 2 6 Turn on the System Fill the wash bottle with wash liquid C
19. e than other rows it means that the corresponding aspirate pin is jammed Doing the maintenance of the wash head following the below steps 1 Twist off the two plastic tubes and the two screws in side of wash head 2 Use two needles to clean the pins of the wash head 3 Take up the wash head with the screw removed end downward knock it against the paper towel or gauze so as to remove the blockage in wash head 4 Dispense the wash head into water plug a large side syringe into the plastics hole on the wash head and push the syringe back and forth Both of the two plastics hole should be done as such 5 Put the black sealing washer round the root of the metal screws and tighten up the screws ATTENTION Cautions Pin tips are sharp and may cause body injury Be careful when you are doing maintenance of the wash head 27 2600C user s Manual 5 3 Disposal of waste bottle The sensor in the waste bottle can detect the level of the waste liquid When it reaches a certain level the bottle will give out warning and system will not receive any operation that includes aspiration At this situation please disposal the waste liquid you can do this without turning off the washer Please follow the below steps 1 Hold the cap of waste bottle and lift whole bottle slightly 2 Hold the bottle with another hand and turn it clockwise until the cap dispatch from the bottle 3 Pour away the waste liquor and put bottle back to its former place 4 H
20. g washing liquid 5 After changing wash head 6 Finding air bubbles in the pipeline If can not clean the air bubbles after prime for one time Please repeat several times to make sure that all the air bubbles are cleaned Besides the above prime procedure started by user the washer 22 2600C user s Manual will prime for one time automatically each time you turned it on Click prime when at the waiting screen the screen displays like this Figure 4 6 Priming After prime the system will back to waiting screen automatically 4 4 Rinsing Click key the screen displays Put the pump in the distilled water press any key to continue Click any key to start the rinsing procedure the screen displays Rinsing Please putting the pump into distilled water and press any key Figure 4 7 Rinsing 4 5 dispense disp At waiting screen click to start dispense settings The menu is as following Dispensing fluid Volume 350 L Figure4 8 Dispensing Click H Et to change the value and click CANCEL to back to the waiting screen The adjustable range of the dispense volume is from 50 L to 2ml The distance for adjustment is 50 L After set the dispense volume click START to the next step 23 2600C user s Manual Strip Setting Figure4 9 Strip setting The number in the center means how many rows from the first row will be washed When using 12pin wash head the maximum number is 8 an
21. late and the work area Treat these areas as potentially biohazardous and or infectious 2600C user s Manual Chapter 1 Introduction 1 1 Intended Use The Microplate Washer is designed for use in processing enzyme linked immunosorbent assays ELISA or EIA including clinical diagnostic assays requiring multistep washing rinsing and soaking This general purpose instrument is intended to be used by laboratory professionals who are capable of selecting the appropriate features and opinions for each specific clinical application ATTENTION Cautions Warning Only qualified personnel should perform service procedures 1 2 Description The 2600C is a microprocessor controlled microplate washing system that performs wash protocols defined by the user The instrument will accept micro wells having flat round or v bottom configurations It is designed to wash all of the wells in one column or one row of a 96 well plate at once The washing protocol can be programmed so that all of the columns or rows are washed in the same manner Or different wash cycles can be applied to specified columns or rows on the plate It can store up to 50 user define programs Feature of the washer 1 Large screen for display graphical interfaces 2 It accept microplate have flat round or v bottom including 8x12 8x6 12x4 microplates 3 Users can program and store them discretionarily 4 It is easy to program Users can set wash times
22. n vertical and horizontal direction within the range of 1mm Please follow the below steps 1 Place the microplate at the work area 2 At the waiting screen double click CANCEL to enter the system setting menu 3 Click to choose Adjusting wash head and click START to enter the submenu The wash head will move to the first row and the pins will get down to the bottom of the microwell automatically The system will display the following screen 4 Click to switch horizontal and vertical click h 2 wash head Please watch carefully the position of the wash head while adjusting its position to adjusting the position of 16 2600C user s Manual At the horizontal direction the aspirate pin long and thick one should be at the center of the microwell At the vertical direction the aspirate pin should be at the bottom of the microwell and there should be a gap of 0 5 1mm between the scabbard of the wash head and the groove of the bracket as shown in Figure 3 5 A ILLI 4 LE Figure 3 5 Adjusting the wash head 5 After adjustment Click the change will be saved into the flashrom of the washer automatically And the wash head will back to the waiting position 6 Click CANCEL to exit during adjusting the wash head will back to the waiting position Adjusting Wash Head Horizontal 0 1mm Vertical 0 1mm Figure 3 6 Adjusting wash head 3 3 5 Specify the speed of diving pump click E m
23. old the cap and put it on the mouth of waste bottle 5 Hold the bottle with another hand and turn counter clockwise until it 1s tightly screwed 5 4 maintenance of tube 1 Fill the wash bottle with distilled water and wash blank plate once a week so as to clean up the whole tube path and dispense pump 2 The liquid water in the wash bottle should cover the dispense pump NOTE e Please pay attention to the power you screw down the cap of the waste bottle If the cap is too loose can t ensure the tightness of waste bottle and affect the effect of aspiration If the cap is too tight it may be damaged e 2600C washer is an exact instrument please pay attention to its daily maintenance e The wash head of the instrument is very exact please pay attention to adding sample and not to add whole blood or other eyewinker otherwise the wash head may be jammed 28 2600C user s Manual Chapter 6 Replacement of Spare Parts 6 1 Replacement of wash head 1 Take the wash head off disconnect the two tubes from the wash head 2 Install a new wash head and connect the tubes then put the wash head on bracket Adjust the wash head to the suitable location ATTENTION Cautions Pin tips are sharp and may cause body injury Be careful when you are installing the wash head 6 2 Replacement of power supply 1 Remove the two screws in the bottom which fix the upper cover and base of the instrument 2 Open the upper cover disassem
24. onnect the 2600C to the laboratory electrical supply outlet Press the instrument power switch at the rear After a series of self test the software version interface will display 13 2600C user s Manual Chapter 3 Settings and Adjustment of Instrument 3 1 System Setting The 2600C washer provides the user with system setting menu for settings of the system These settings are necessary for the normal use of the washer ATTENTION Cautions If the system setting is not correct it will cause serious aftereffect such as wrong dispense volume liquid overflow damage to the wash head and so on Please be careful To enter the system setting menu just double click the cancel key at the waiting window of the washer Its menu is as following Adjusting Contrast Adjusting wash head Adjusting Top Aspr Adjusting Pump Figure 3 1 System setting menu Press SELECT to select the sub menu press START to enter 3 3 1 Adjust the contrast At the system setting menu select Adjusting Contrast and press start Contrast Figure 3 2 Adjusting contrast 14 Press to increase and press to decrease contrast After adjusted to the best value Press START to save and exit The value will be saved in the flashrom of the washer the contrast of LCD will be set as new value the next time you turn on the washer If you press after adjustment the value will not be saved There are 64 rank of the washer L
25. re changing the wash head Never touch the pins while the instrument is operating The bottles If the washer is giving out a warning that the waste bottle is full during the operation immediately set the power switch to OFF Then you can disconnect the bottle and pour away the waste liquid The bottles are pressurized during normal operation Do not remove bottle caps or tubing connections while the bottles are pressurized Set the power switch to OFF before changing bottles or tubing connections 2600C user s Manual If the waste bottle overturns while the instrument is running immediately set the power switch to off At this situation the washer may give out some waste liquid through the waste bottle from a device at the bottom of the instrument They must be treated as biohazardous materials Biohazardous Materials Some diagnostic assays utilize material which is potentially biohazardous Always wear protective apparel and eye protection while using this instrument Always operate the instrument with the aerosol shield lowered Waste liquid Avoid direct contact with waste solution and or solid waste Both should be handled as potentially biohazardous Dispose of waste solution and or solid waste according to the relevant governmental regulations Consult the reagent manufacturer for information on the concentrations of heavy metals and other toxic constituents in each reagent Biohazardous parts Avoid direct contact with the microp
26. turn off the instrument directly ATTENTION Cautions You must wait for at least 30 seconds if you want to turn on the instrument again 19 2600C user s Manual Chapter 4 Operation of Instrument 4 1 Work area and microplate The work area of the washer is shown in the following figure The rear end of the work area is the waiting groove When the washer is at the waiting state the wash head is in the groove The front end of the work area is the objective table ATTENTION Cautions e Only when the instrument is at the waiting state you can place microplate on or take it out of the objective table Otherwise it will cause damage to the instrument or you will be hurted e Please wear gloves while you are operating the washer and don t touch the patients samples directly If your wound touched patient s sample by accident please wash immediately and go to doctor for help Figure4 1 Work area The 2600C washer can wash 12x8 12x4 8x6 microplates But different wash head will have some limit on the kind of microplate The relation is as follows Wash head l Spin 12pin microplate 12x8 Support Support 12x4 Not support Support 8x6 Support Not support Please pay attention to the direction of microplate when you are placing it to the objective table If 20 2600C user s Manual you are using Spin wash head you should make the eight microwell edge of the microplate parallel with the
27. wash head Similarly if you are using 12pin wash head you should make the 12 microwell edge of the microplate parallel with the wash head When you are placing the microplate to the objective table hold the two edge of the microplate put it into the crossed groove from above to below Make sure that the back edge contact tightly with the bake edge of the groove ATTENTION Cautions The instruction for placing microplate must be followed Otherwise it will cause bad aftereffect such as wrong dispense volume Liquid overflow damage to wash head and so on 4 2 Wash procedure In order to bring convenience to different wash procedure 2600C washer provides you with the user define program There are total 50 programs numbered from 0 50 and they are stored in the flashrom of the washer The user define program is consist of the follow parameters Wash mode Select from plate and strip Plate mode is to dispense and aspirate by whole plate Strip mode is to finish the whole procedure including dispense soak and aspirate by each row Aspirate mode Select from single and double Single is that the pins only stay at the center of the microwell one time and aspirate Double is that the pins stay at both the two edges of the microwell and aspirate Dispense volume The dispense volume for each microwell If you choose 8pin wash head the select range is 50 L 3mL If you choose 12pin wash head the select range is 50 L 2mL The

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