2563 Service Manual.pmd - Triangle Biomedical Sciences
Contents
1. Following a defrost some ice will remain in the drip tray below the evaporator at the rear of the chamber When the light in this button is illuminated a defrost is in progress Press the FREEZE PLATE TEMP button to view the quick freeze plate temperature An illuminated light in this button indicates the quick freeze plate temperature is displayed Press the CHAMBER TEMP button to view and change the desired chamber temperature Ifthe display is indicating the freeze plate temperature then pressing the CHAMBER TEMP button returns the display to the actual chamber temperature Pressing the CHAMBER TEMP button again will cause the display to flash indicating it is in the change mode Use the up and down arrow keys to change the display to the desired temperature When the desired temperature is reached simply press the CHAMBER TEMP button again and the display will revert to the actual chamber temperature An illuminated light in this button indicates the actual chamber temperature is being displayed Minotome Plus Operation Manual The arrow buttons are used along with the time and temperature control buttons for setting the desired parameters The DEFROST SET button is used to select the desired automatic defrost time This time is set for 1 00 AM from the factory If a different time is desired press the DEFROST SET button once and use the up and down arrow keys to change the hour display to the desired time Press the DEFROST SET bu2. by sliding it left Locate the set screw beneath the front of the assembly and ensure that itis tight Replace the assembly in the microtome base 2 4 Specimen Holder Angle Adjustment Locking Lever Install the specimen orientation ball l S assembly and detachable specimen en holder as shown Lightly tighten the Handle specimen orientation handle so thatthe ball assembly is still moveable Be certain the knurled screw is tight Position the loose specimen orientation ball assembly so that the detachable specimen holder is completely vertical with respect to the blade edge facet Place a flat ruler vertically on top of the knife holder screws and slide the knife holder Detachable Specimen forward to engage the loose Specimen Orientation assembly Tighten the specimen Holder Ball Assembly orientation handle to set the angle Minotome Plus Operation Manual 7 2 5 Optional Knife The resharpenable knife Cat 3257 is an option and can be used as an alternative to the Disposable Blade System A modification of the anti roll plate is required see section 2 6 The installation and cutting angle adjustment procedures are similar for reusable knives and the Shur Sharp disposable blade system although the knife holder screws may require more threads showing in order to compensate for the different cutting angles of reusable knives 2 6 Anti Roll Plate Alignment Proper adjustment of the anti roll plate may be made onc
3. extreme caution when handling Although the microtome is installed at the factory its alignment may shift during transport Misalignment results in a clunking sound when the handwheel is turned To realign loosen the two large black thumbscrews which secure the microtome to the cryostat Loosen the two set screws that couple the outer handwheel shaft to the inner microtome shaft Pull the handwheel away from the cryostat Check that the handwheel is securely fastened to the outer shaft by holding each and turning to feel for any play between them The handwheel is secured by two set screws which must be aligned with flat surfaces on the outer shaft If any looseness is found align and tighten the set screws Slide the handwheel back toward the cryostat taking care to align the set screws in the outer handwheel shaft with the flat surfaces of the inner microtome shaft and tighten Rotate the handwheel and reposition the microtome until no clunking sound is heard Then while rotating the handwheel slowly alternately tighten down the large black thumb screws in small increments Rotate the handwheel Feel for any binding If the turning action is difficult the outer shaft may need to be freed up by loosening the wedge outside of cabinet or seal inside cabinet Once either is loosened rotate the handwheel to free up the shaft and secure the wedge or seal back in place both should be centered about the shaft Set Screws Handw
4. the horizontal sliding surfaces 4 5 Warranty TRIANGLE BIOMEDICAL SCIENCES INC TBS for itself and all its subsidiaries does hereby warrant for a period of one year 12 months from the date of shipping under normal and proper usage all of its products against defects in workmanship and material and will repair or replace the same without charge when shipped prepaid to TBS s factory in Durham NC USA If the product cannot be so shipped except for parts and accessories there will be a charge for all expenses incurred for repairing or replacing the product on the user s premises Parts and accessories manufactured or supplied by others are warranted only under the regular warranty of the manufacturer or supplier of such parts in so far as TBS or its subsidiaries are able to transfer the benefit of such warranties to the user This warranty supersedes and is given in lieu of all implied warranties and is void if user does not provide unit with continuous ample electrical power at constant voltage consistent with the specifications of the product User is required to return the warranty card to TBS within ten 10 days after receipt of the product in order to validate the warranty 4 6 Condition of Returned Equipment Before returning equipment to TBS you must contact TBS or your dealer and receive areturn goods authorization All returned units must be decontaminated free of radioactivity and free of hazardous and infecti
5. RFFERREHFEFEESFRFLFERERDETEREEEERR 19 4 4 LUD MIC ARON EEN 19 4 5 Warranty ZRASESRBRSREREFERRABEREERABSAFEEROERELTERRESEELTEERTPERTEROFTLEEREELSELTT 21 4 6 Condition of Returned Equipment 21 SPECIFICA TONS au 22 5 1 Temperature Selection Table 23 Minotome Plus Operation Manual 1 0 SIMPLIFIED OPERATING INSTRUCTIONS General The TBS Minotome Plus is designed for use as a cryogenic sectioning device in clinical pathological and research applications Do not attempt to use the instrument unless you are professionally trained in the use of similar devices Installation Rollthe cryostat to the desired location and lock the front casters Allow a minimum of 4 inches clearance on all sides of the cryostat for proper ventilation Allow additional clearance on the right side for easy rotation of the handwheel The international model Cat NO 2564 is shipped for use at 240 VAC To use it on a different voltage source the autotransformer must be reconfigured A qualified electrical technician should use an AC Voltmeter to measure the voltage at the installation site After removing the rear panel relocate the BROWN wire to the autotransformer tap which is closest to the measured line voltage Replace the rear panel To minimize line load variations the Minotome Plus should be connected to a dedicated line The line voltage supply must be a standard three wire grounded single phase circuit Ope ration Select an appropriate chamber temp
6. Screw 2 eh Anti Roll Adjust and Lock Knife Holder Locking Knife Holder Support Minotome Plus Operation Manual 15 3 4 Troubleshooting Problem Possible Cause No Lights Blown fuse on circuit board No Cooling Refrigerant problem Chamber temp rising Sectioning too long Evaporator Blocked No motor action Loose wiring Microtome not advancing Ratchet pawl in manual position no clicks heard Weak pawl spring Frost on ratchet wheel Inconsistant sections Loose blade holder Thick and Thin Loose specimen Dull Blade Improper Blade Holder angle Loose Microtome Sections fragment Toocold Knicked or damaged Anti roll plate Sections gather Toowarm at knife edge Anti roll plate incorrectly positioned Knicked or damaged Anti roll plate Dull Blade Handwheel hard to turn Dry Microtome Shafts misaligned Wedge or seal binding 16 Corrective Action Callforservice Call for service Close window Defrost Check motor connector Moveto automatic Replace spring Clean ratchet wheel Tighten locking lever Tighten specimen holder screw Replace resharpen Adjust angle see Sec 2 2 Tighten thumbscrews set screws Raise chamber temperature Replace anti roll plate Lowerchamber temperature Adjust height see Sec 2 6 Replace anti roll plate Replace resharpen Lubricate Align see Sec 2 1 Adjust see Sec 2 1 Minotome Plus Operation Manual 4 1 Cleaning 4 0 MAINTENANCE The Minotome P
7. TBS TRIANGLE BIOMEDICAL SCIENCES INC 3014 Croasdaile Drive Durham NC 27705 USA Ph 919 384 9393 Fax 919 384 9595 E Mail tbs trianglebiomedical com Minotome Plus OPERATION MANUAL OM2563 Version 1 1 Microtome Cryostat Cat 2563 115V 60Hz 100V 50Hz Cat 2564 240V 50Hz 200V 50Hz 4 H d SSS Minotome Plus Operation Manual 1 1 21 98 1 0 2 0 3 0 4 0 5 0 Table of Contents SIMPLIFIED OPERATING INSTRUCTIONS rss 3 INSTALLATION u g s ee Set egegkeiegk Eege iin 5 2 1 Microtome Adjustment 6 Microtome Alignment nn 6 2 2 Disposable Blade Holder Installation 7 2 3 Positioning of Knife Holder Assembly 7 2 4 Specimen Holder Angle Adjustment 7 2 5 Optional Knife ss 8 2 6 Anti Roll Plate Alignment 8 2 7 Optional Index Post 10 OPERATIONS 2 een nes me eee 11 3 1 Warnings and Cautions 11 3 2 Control T ss 12 3 3 DOCTIONINQ DEE 14 allt E 14 Temperature Selection 14 Thickness Selection 14 Sample Preparation 14 Tale HTHH 15 3 4 Troubleshooting s ssss 16 MAINTENANCE u een 17 4 1 Lu e si ence rete ice gs fetes ane tec teen et 17 4 2 DECONTAMINATION T 18 4 3 Spate PANS PPRBSREPFIERFDEFFEREFFPFEERROSERFEEF
8. The front control panel of the Minotome Plus is used to set chamber RETRACT ADVANCE Q Q CLOCK DEFROST SET SET TEMPERATURE Q Q CHAMBER FRECZE TEMP PLATE TEMP parameters and activate the chamber controls F ILI cree r CHAMBER CONTROLS Q D Q MANUAL QUICK CHAMBER DEFROST FREEZE LIGHT 12 S CHAMBER LIGHT O QUICK FREEZE O MANUAL DEFROST O FREEZE O CHAMBER TEMP Press the CHAMBER LIGHT button to illuminate or shut off the fluorescent lamp inside the cutting chamber The chamber light is on when the light in the button is illuminated Press the QUICK FREEZE button to deliver refrigerant to the quick freeze bar on the left side of the chamber for 10 minutes to provide rapid sample preparation When pressed again this function is terminated The quick freeze is activated when the light in the button is illuminated Note During normal cooling the refrigerant is cycled through the quick freeze plate This function activates refrigeration when it has cycled off or extends it for a period of 10 minutes Once stopped there is an intentional three minute delay before the compressor can be re started Press the MANUAL DEFROST button to activate a 3 minute approx defrost cycle A defrost cycle may be terminated by pressing the button again Periodic manual defrost in addition to the automatic defrost is recommended if climate conditions are hot and or humid
9. active whenever the compressor is running Prolonged contact with unprotected skin may result in burns TBS warrants for a period of one year 12 months from the date of shipping under normal and proper usage all of its products against defects in workmanship and material and will repair or replace the same without charge when shipped prepaid to TBS factory in Durham NC User is required to return the warranty card to TBS within ten 10 days after receipt of the product in order to validate the warranty Warranty service for international shipments is handled by the selling agent For further information please call 919 384 9393 Minotome Plus Operation Manual 2 0 INSTALLATION Follow the instructions on the outside of the shipping carton to unpack the cryostat The Minotome Plus is shipped with the microtome installed in the cryostat chamber and the Shur Sharp Blade Holder and anti roll device in position Roll the cryostat to the desired location and lock the front casters by pushing the small locking lever on the top of each wheel with your foot Allow a minimum of four inches clearance on all sides of the cryostat for proper ventilation Allow additional clearance on the right side for easy rotation of the handwheel The international model Cat No 2564 is shipped for use at 240 VAC To use it on a different voltage source the autotransformer must be reconfigured Only a qualified electrical technician should make the f
10. bs Note Typical sound level emissions do not exceed 70 dB A Specifications Subject To Change Without Notice Minotome Plus Operation Manual Temperature Selection Table In Minus C Adipose Tissue pp pf Adrenal fag pp PP Bladder Bone Marrow J J J fig Bren pp PP Breast With fat Breast without fat AU TI Lymph Nede Lymphatic AU TI Lymphoid II TI Muscle pp pg PG Noe en Omentum PP O oe ll 1 ME EE Pancreas 1 pf ES Prostrate Rectal 1 pf PG Scrapings 1 PJ pp Skin withfat Skin without fat J EE Soft Parenchyma Lesion To S S Spleen pp PP Testicular J Jo o S T S S Uterine Curettings pp pp teus pp pp pg Minotome Plus Operation Manual 23
11. e the upper edge of the plate is aligned parallel to the edge of the disposable blade or reusable knife To accomplish this loosen the two slotted screws on the right side mounting support The assembly can then be shifted until the edges are parallel Tighten the two slotted screws once the plate is aligned The height of the anti roll plate relative to the blade is also important If the plate is too high the sample will hit the plate instead of the blade If the plate is too low the sample will curl up in front of the plate The height is adjusted by first loosening the small thumbscrew lock Next turn the adjustment screw by its rubber cap to raise or lower the plate Once the samples feed smoothly between the plate and blade tighten the thumb screw lock Note A dirty or damaged nicked upper edge anti roll plate will cause the sample to tear or bind Additional Slotted Screws Round head Screw anti roll plates Cat 3267A can be ordered Acorn Nut All anti roll plates are shipped from the factory configured for the Disposable Blade Holder To reconfigure the anti roll plate assembly for reusable knives 1 Loosen do not remove the round head screw acorn nut on the right side of the anti roll plate mounting support 2 Remove the anti roll plate assembly Be careful to restrain the spring while disassembling Wear protective glasses Take it to a bench and rework it as follows Use needle nose pliers or fo
12. erature Insert a disposable blade H DMB HD into the Shur Sharp disposable blade holder already installed in the cryostat Loosen the thickness lock at the top of the index post and rotate the index so a setting lines up with the etched guide Settings of 1 through 8 may be selected where 1 corresponds to 2 microns and 8 corresponds to 16 microns Place the specimen holder into the ball and chuck assembly so the notch on the outer edge of the specimen holder lines up with the knurled screw Turn the knurled screw until it is finger tight Advance the sample until it is approximately 1 8 inch from the cutting edge using one of the following methods 1 Motorized Press the ADVANCE RETRACT switch downward and hold 2 Manual Turn the large ratchet wheel at the rear of the microtome clockwise being careful of the blade 3 Knife Holder Loosen the knife holder assembly locking lever by moving it to the right and slide the assembly forward Return the locking lever to the original locking position Rotate the anti roll plate away from the disposable blade Ensure that the ratchet pawl on the large ratchet wheel at the rear of the microtome is in the engaged position toward the right Rotate the handwheel several times until the blade contacts the sample Make several initial cuts until the desired sectioning face is obtained Rotate the anti roll plate back into position to begin sectioning Minotome Plus Operation Manual 3 Wa
13. heel Set Screws Outer Shaft Wedge Coupling Minotome Plus Operation Manual 2 2 Disposable Blade Holder Installation A Shur Sharp Disposable Blade Holder and blades are standard items supplied with the Minotome Plus The anti roll plate is configured for use with the disposable blade system To reconfigure the anti roll mechanism for use with reusable knives see section 2 6 Specimen Holder Warning Disposable blades and reusable knives are extremely sharp To prevent injury use Disposable Blade extreme caution when handling Yero ote Back the two knife holder screws out until they are no longer visible from the front of the unit Advance one knife holder screw until one thread is visible Some units may require up to two threads to be visible Secure screw in place by tightening the lock nut around it Repeat procedure for remaining screw Tighten both knife clamps down Lock Nut Holder Bracket 2 3 Knife Holder Assembly Adjustment When the Knife Holder Assembly locking lever at front and bottom of knife holder assembly is tightened down slid left the entire assembly should be immobile no sideways motion unable to slide forward or backward If itis mobile remove the assembly from the microtome base and slide the locking lever to the right Using a flathead screwdriver rotate the center bolt counter clockwise not too tight Be sure that the locking lever can still be tightened
14. l 13 3 3 Sectioning First Use Temperature Selection Thickness Selection Sample Preparation Sample Position 14 The specimen holder assembly can only be retracted if the ratchet pawl is in the manual position Caution The ratchet pawl must be in the manual position to allow the specimen holder assembly to retract The motor will not be able to retract otherwise and will emit aloud noise Always place the ratchet pawl in the manual position before retracting to avoid damaging the microtome motor Once the ratchet pawl is in the manual position press and hold the toggle switch upward to retract the specimen holder assembly Once it is fully retracted the motor will emit a loud noise indicating it cannot retract any further When the Minotome Plus has been plugged in and a chamber temperature has been selected it takes approximately one hour to achieve an air temperature of 20 C It will take several hours for all the metal mass of the microtome and blade holder to reach equilibrium For best results do not rush the process Once set temperature is reached the microtome may require adjustment see Section 2 1 Microtome Adjustment and lubrication see Section 4 4 Lubrication Select an appropriate chamber temperature according to the chart at the rear of this manual If the sample fragments or splits when sectioning the se lected temperature may be too cold If the selected temperature is too warm the
15. lus should be unplugged and allowed to warm up to room temperature before cleaning Remove the disposable blade system and any blades stored in the chamber To prevent rusting run them under warm water until they are room temperature and dry thoroughly Remove rubber drain plug from bottom of inner chamber Warning The microtome disposable blades are very sharp To prevent injury use extreme caution when handling Clean the outside of the Minotome Plus and the inside of the chamber with adamp sponge using a mild detergent non abrasive The microtome must be removed for cleaning Loosen the two set screws that couple the outer and inner shafts and pull the handwheel out Loosen and remove the large black thumbscrews which secure the microtome to the chamber Disconnect the motor wiring Carefully lift the microtome out of the chamber Note The microtome weighs approximately 45 pounds Before cleaning the microtome remove the motor by loosening the set screw that holds it in place The microtome can be cleaned with absolute alcohol If any water based solutions are used the microtome must be dried thoroughly using alcohol or a source of warm dry air Note The microtome must be lubricated after cleaning Install the motor taking care to line up the flat side of the shaft with the set screw Place the microtome into the crystat chamber and install according to the instructions in Section 2 1 Microtome Adjustment Re connect the mo
16. notome Plus Operation Manual The sample can be brought closer to the blade edge by one of the following methods Motorized Press the ADVANCE RETRACT switch downward and hold Manual Turn the large ratchet wheel at the rear of the microtome clockwise being careful of the blade Knife Holder Loosen the knife holder locking lever by moving it to the right and slide the knife holder assembly forward Use any method to advance until the Disposable Blade Edge is approximately 1 8 inch from the sample Rotate the anti roll plate away from the blade Cutting Ensure that the ratchet pawl is in the automatic position toward the right Rotate the handwheel several times until the blade engages the sample Note To advance more quickly using the handwheel rotate the handwheel from 12 o clock to 3 o clock and then back to 12 Each of these forward movements advances the sample without making a pass by the blade Make several rough cuts until the desired sectioning face is obtained Rotate the anti roll plate back into position to begin sectioning At this point each turn of the handwheel will produce one section Specimen Holder Clamp Ball Chuck Specimen Holder ad ef Specimen Holder Specimen Holder Clamp Specimen Holder Clamp Knob Coarse Advance Retract Knife Angle Set Screw and Lock 2 Anti Roll Plate Thickness Adjust Knife Clamp Finger 2 Index Post ndex Pos b nn 1 Knite Clamp SPS Finger
17. o that it will be parallel to the knife edge Tighten both slotted screws NOTE If sections curl up in front of the anti roll plate then the plate must be adjusted forward If a specimen hits the plate then the plate is too far forward and must be adjusted backward NOTE The knife angle may need readjustment At this point you are ready to start sectioning IMPORTANT Do not store or leave a blade in place for extended periods Leave the blade holder clamp in the open position when not in use STANDARD KNIFE DISPOSABLE BLADE HOLDER Disposable Blade Knife clamp finger 2 Blade Holder et 3434 OPEN Lever Closed position Piastic washer finger screw 2 Spring Right side slotted screws Mounting support 2 Knite holder locking lever Thumbscrew lock for anti roll Thumbscrew lock for anti roll Anti roll adjust Minotome Plus Operation Manual 9 2 7 Optional Index Post 10 Optional index post Cat No 3347 allows the user to section 18 to 32 um or 34 to 42 um To install locate and loosen the set screw at the rear of the microtome base which secures the index post position Remove the original post and install the optional post with the flat portion toward the rear and the red lines facing the user Adjust the height of the post so one red line is flush with the top surface of the base Use lower red line for 18 to 32 um and the upper red line for 34 to 42 um Rotate the handwheel and listen
18. ollowing adjustments Using an AC Voltmeter measure the voltage at your site Remove the rear panel Relocate the BROWN wire to the autotransformer tap which is closest to the measured line voltage Replace the rear panel To minimize line load variations the Minotome Plus should be connected to a dedicated line The line voltage supply must be a standard three wire grounded single phase circuit Warning The power cord must be plugged into a grounded power outlet Never remove the grounding prong from the power plug or use any adapter which defeats or does not complete the power ground circuit The power cord provided with the Minotome Plus is correctly rated for the highest current demand This power cord should never be interchanged with cords from equipment with lower current demand Incorrect power cords can create a fire hazard Plug the cryostat in and review the Control Panel settings section 3 2 If you encounter difficulty sectioning follow the more detailed Microtome Adjustment instructions section 2 1 Minotome Plus Operation Manual 2 1 Microtome Adjustment Microtome Alignment The microtome is installed in the cryostat at the factory but may require some adjustment on site for proper sectioning The following adjustments may be made to correct a common problem of thick and thin or inconsistent sectioning Warning Disposable microtome blades and reusable knives are extremely sharp To prevent injury use
19. ous materials The RGA paperwork includes a Certificate of Decontamination for you to sign indicating that you have performed these steps TBS will not accept the shipment until this signed certificate is received You must prepay transportation to the service depot In addition TBS must receive unit packed and shipped in compliance with our shipping crating instructions These can be faxed upon request Failure to properly crate cryostat and ship with handwheel locked could result in additional repair fees Minotome Plus Operation Manual 21 5 0 Microtome Type Section Thickness Increments Cryostat Temperature ControlRange Increments Cool Down Quick Freeze Temperature Refrigerant Defrost Heat Output typical Power Reqirements Cat No s 2563 amp 2574 Cat No s 2564 amp 2575 Dimensions Height Width Depth Packaging Dimensions Height Width Depth Weight Net Shipping SPECIFICATIONS Minot rotary 2 to 16 microns standard 18 to 42 microns optional two microns Ambient to 30 C 20 C ambient 1 C Ambient to 20 C in 60 minutes 20 C ambient Approximately 40 C Refrigerant type shown on dataplate Manual or Automatic 2000BTU hr 115V 10 60 Hz single phase 15A 100V 10 50 Hz single phase 15A 200 240V 10 50 Hz single phase 7 5A 114 cm 45 in 65 cm 25 5 in 69 cm 27 in 142 cm 56 in 81 cm 32 in 105 cm 41 in 122 kg 269 Ibs 159 kg 350 I
20. rceps to remove the E ring Remove anti roll plate plastic washer and spring Reassemble the anti roll plate parts in the following order first put anti roll plate followed by the spring next the plastic washer and finally put the E ring back into its original slot to hold the parts together 3 Putthe reworked anti roll plate assembly back into its mounting supports so that the thumbscrew lock now faces up This is very important because the anti roll plate is now inverted from its original position Turn the round head screw just enough to hold the anti roll plate in place but do not tighten them against the side of the anti roll plate support yet 8 Minotome Plus Operation Manual Install the reusable knife as follows 1 Loosen both knurled knife clamp finger screws 2 WARNING The top edge of the knife is very sharp Very carefully remove the knife Place it so that it is centered between the microtome knife clamp fingers 3 Tighten both knurled knife clamp finger screws 4 Use the anti roll adjust and lock knobs to align and lock the anti roll plate just above the knife edge to prevent specimen curling The anti roll plate should be parallel to the knife edge If itis tighten both slotted screws on the right side of the anti roll plate mounting support If not adjust it as follows Loosen but do not remove both slotted screws on the right side of the anti roll plate mounting support Adjust the anti roll plate s
21. rnings amp Cautions Warranty Service Below are listed important operating procedures Be certain to follow the instructions carefully Do not locate the Minotome Plus in direct sunlight or near any air handling ducts The microtome should always be lubricated after cleaning and or monthly Do so at operating temperature Lubricate with TBS s Shur Lube H LUB When sectioning for extended periods of time the chamber temperature may rise Close the window and allow the cryostat time to recover There is no automatic stop to prevent the main feed screw from advancing too far completely out Retract the assembly regularly to avoid damage to the microtome Always put the ratchet lever in the manual position before retracting in order to avoid damaging the microtome motor Before decontaminating the microtome be sure to remove the motor assembly from the rear of microtome The Shur Sharp disposable microtome blades are very sharp To preventinjury use extreme caution when handling Carefully remove and properly discard spent blades If infectious or radioactive material have been sectioned immediately sterilize or decontaminate the Minotome Plus and its components The power cord must be plugged into a grounded power outlet Never remove the grounding prong from the power plug or use any adapter which defeats or does not complete the power ground circuit The quick freeze plate is extremely cold and is
22. sample will seem soft and sections will collect at the blade edge The index post is used to set the desired section thickness Loosen the thickness lock at the top of the index post and rotate the index adjust so a setting lines up with the etched guide Settings of 1 through 8 may be selected Refer to the tables in Section 2 7 to identify thickness by setting number The holes in the quick freeze plate on the left side of the chamber can accommodate up to 13 specimen holders for sample preparation When the refrigeration system is on the quick freeze plate temperature is approximately 40 C Ifit is not on press the QUICK FREEZE button on the control panel and the system will cycle on a three minute delay between cycles may slow the start Place a small amount of embedding medium TFM ona specimen holder When it starts to turn white and solidify place the specimen on the plate and surround it with more embedding medium Allow it to freeze thoroughly before sectioning The heat extractor Cat 2085 can be used to accelerate the process The specimen holder angle should be vertical see Section 2 1 Microtome Adjustment before sectioning Place the specimen holder into the ball and chuck assembly so the notch on the outer edge of the specimen holder lines up with the knurled screw When knurled screw is tightened it should engage asmall hole This prevents looseness in the section mounting Tighten the knurled screw finger tight Mi
23. staining procedure 6 When technical procedure is completed put on clean gloves and place residual tissue in 10 formalin to fix thoroughly before it is handled again Decontamination of Cryostat The AIDS virus is known to be inactivated by 0 5 sodium hypochlorite 1 10 dilution of household bleach 3 4 95 ethanol 3 4 5 6 and 10 formalin 6 Sodium hypochlorite is the most effective decontaminant known for the virus but it will also corrode metal Therefore 95 ethanol is favored for the inside of the cryostat The AIDS virus does survive both freezing and drying 4 6 so decontamination is essential Turn off refrigeration Allow chamber to warm to room temperature 2 With protective clothing in place remove the blade from microtome and place it in a pan of 95 ethanol on the floor of the cryostat chamber where water will collect 3 If complete defrosting is desired pour a small amount of 95 ethanol on the floor of the cryostat chamber where water will collect 4 Wipe all exposed surfaces inside and outside the cryostat chamber with a towel or sponge that has been soaked in 95 ethanol 5 Replace the disposable blade holder or knife from freezer 6 Turn on refrigeration for return to desired temperature Another decontamination technique recommendedbefore the ethanol sensitivity of the AIDS virus was known consists of placing a large beaker of 40 formalin in the closed and defrosting chamber overnight However
24. the moveable parts must be lubricated before the cryostat is used again Minotome Plus Operation Manual Cleanup of Work Area Personnel not directly involved with preparing frozen section and decontaminat ing the cryostat must also be protected from exposure to the AIDS virus Do not touch other objects in room while wearing contaminated gloves 2 Place instrument in 0 5 sodium hypochlorite for 15 minutes wash well in tap water and autoclave 3 Wipe bench top with 0 5 sodium hypochlorite 4 Place all contaminated towels protective clothing etc in double plastic bags Use separate containers for disposable and reusable items Bags MUST NOT contain sharp objects 5 Contaminated garments should never be worn outside the frozen section room 6 Attach biohazard labels to bags 7 Wash hands before leaving the area 4 3 Spare Parts Part No Description 3347 Index Post 18 to 42 microns 3434 Disposable Blade Holder H DMB HD Disposable Blades heavy duty H DMBH Disposable Blade Holder H LUB Shur Lube Microtome Lubricating Oil 3331 Specimen Holder Concentric Circles 3408 Specimen Holder Cross Hatch H TFM Tissue Freezing Medium H FRZ 1 Shur Freeze Cryogen Spray 3267 Anti roll Plate with Feed Screw 3267A Anti roll Plate without Feed Screw H ARD Anti roll Device 3330 Ball Chuck for specimen holders 3331 and 3408 3375 Replacement Anti roll Assembly 2085 Heat Extractor H BR Brushes Camel Hair 1 8 1 4 3 8 wide bris
25. tles 4 4 Lubrication Lubrication of the microtome is important to maintain smooth trouble free sectioning The microtome should be lubricated regularly based on usage For example if the microtome is used daily lubricate it weekly If the microtome is used weekly lubricate it monthly Shur Lub has a proper blend of lubricants to protect the microtome The microtome components which require lubrication are shown in the following diagrams They include the inner shaft bearings 1 the blade holder assembly sliding surfaces 2 the dovetail upright gibs 3 the main feed screw and sliding block 4 When lubricating the bearings 1 the dimples must first be pressed down to allow oil to flow into the bearings A broken paperclip is ideal for the purpose When lubricating the blade holder sliding surfaces 2 loosen the locking lever and slide the assembly out Place one drop on either side of the track slide the assembly back in and tighten the locking lever Minotome Plus Operation Manual 19 Minotome Plus Operation Manual 20 When lubricating the upright gibs position the handwheel so that the specimen holder assembly is at its lowest height Place a couple of drops on either side of each dovetail and allow it to run down the upright gib When lubricating the apertures in the feed screw and sliding block 4 puta small paperclip or pin into each hole Drip oil down it into the aperture Place a few drops on
26. to ensure that the correct number of clicks can be heard for each setting see table Standard Index Post 2 to 16 um Setting No of Clicks Microns Advanced 1 1 2 2 2 4 3 3 6 4 4 8 5 5 10 6 6 12 7 7 14 8 8 16 Optional Index Post 18 to 32 um lower red line Setting No of Clicks Microns Advanced 1 9 18 2 10 20 3 11 22 4 12 24 5 13 26 6 14 28 7 15 30 8 16 2 Index Post 34 to 42 um upper red line Setting No of Clicks Microns Advanced 1 17 34 2 18 36 3 19 38 4 20 40 5 21 42 Maximum setting See Caution Caution Do not set the microtome to advance more than 42 um A greater setting will not allow the specimen to clear the blade before advancing Minotome Plus Operation Manual 3 0 OPERATION 3 1 Warnings and Cautions Disposable blades and reuseable knives are very sharp To prevent injury use extreme caution when handling S If infectious or radioactive material has been sectioned IMMEDIATELY sterilize or decontaminate the Minotome Plus and its components S To avoid electrical shock the power cord must be plugged into a grounded power outlet Never remove the grounding prong from the power plug or use any adapter which defeats or does not complete the power ground circuit The quick freeze plate is extremely cold and is active whenever the compressor is running Prolonged contact with unprotected skin may result in burns Do not position the Mino
27. tome Plus in direct sunlight or near any air handling ducts Allow four inches of space on all sides of the Minotome Plus for proper ventilation N The microtome requires periodic lubrication based on usage It should always be lubricated after cleaning The procedure should be done once it has reached operating temperature d Lubricate the microtome with Shur Lube H LUB a specialty chemical formulation designed to lubricate the various metals utilized under sub zero operating conditions When sectioning for extended periods of time the chamber temperature may rise Close the window and allow the cryostat time to recover if the sections become mushy N There is no automatic stop to prevent the main feed screw from advancing too far completely out Retract the assembly regularly to avoid damage to the microtome N The ratchet lever must be in the manual toward the left position to allow the specimen holder assembly to retract The motor will not be able to retract otherwise and will emit a loud noise Always put the ratchet lever in the manual position before retracting to avoid damaging the microtome motor Return the ratchet lever to the right engaged for continued cutting Before decontaminating the microtome be sure to remove the motor assembly It may not be compatible with some processes of decontamination See Section 4 2 Decontamination Minotome Plus Operation Manual 17 3 2 Control Panel
28. tor wiring Replace rubber drain plug Periodically the air flow path for the refrigeration system should be checked for any restrictions dust dirt Remove the access panel on the left side of the cryostat and clean out the condenser Also ensure that the vents on the right side are free of dust or dirt Minotome Plus Operation Manual 17 4 2 Decontamination 18 If infectious tissue has been sectioned IMMEDIATELY decontaminate the work area and the inside of the microtome cryostat chamber as cited by Billy L Swisher and Edwin P Ewing Jr in the article entitled Frozen Sectioning Techniques for Tissues Infected by the Aids Virus in the Journal of Histotechnology Vol 9 No 1 March 1986 page 29 Specimen Handling Infections by the AIDS virus are known to occur only by direct contamination of the blood 1 or mucosal surfaces 2 Therefore safety measures are directed toward prevention of cuts and protection of the eyes nose and mouth Protective Clothing 1 Doublegloves 2 Eyeglasses or goggles 3 Mask 4 Gown Technique 1 Confine possible benchtop contamination to small area 2 Drop mounting medium onto specimen holder plate and at the appropriate time add tissue DO NOT allow dispenser to touch tissue 3 DO NOT create any aerosol in the chamber 4 Briefly immerse entire frozen section slide in 10 formalin 5 Remove double gloves withdraw slide from formalin with clean forceps and proceed with
29. tton again and use the arrow keys to change the minute display When the desired defrost time is reached simply press the DEFROST SET button and the display will revert to the current time The automatic defrost time is in the change mode when the light in the button is illuminated DEFROST ET a e The CLOCK SET button is used to change the displayed time The time is set for Eastern Standard Time from the factory If a different time is required press the CLOCK SET button and use the up and down arrow keys to change the CLOCK hour display Press the CLOCK SET button again and use the arrow keys to SET change the minute display When the current time is reached simply press the CLOCK SET button and the display will indicate the current time An illuminated light in this button indicates the current time displayed is in the change mode 9 The RETRACT ADVANCE switch is used to advance or retract the specimen holder assembly The specimen holder assembly can be advanced at any time To ensure rigidity when sectioning the specimen holder assembly should never be extended more than 1 2 inch Caution There is no automatic stop to prevent the main feed screw from advancing too far completely out Retract the assembly regularly to avoid damage to the microtome To coarse advance press and hold the toggle switch downward To advance approximately 20 um only depress the toggle switch once and release Minotome Plus Operation Manua
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