Heraeus Biofuge Pico - User manual
Contents
1. Once the rotor is in place the mains switch turned on and the lid closed you can start the centrifuge Press the start key 2 in the control panel The centri fuge accelerates to the preselected value Simultane ously the run time display starts going backward from the preset time giving the remaining run time in min utes After reaching the last minute the display switches to seconds remaining During the run the ro tating light in the speed panel signals that the centri fuge is running During the run you cannot open the lid Changing the settings during the run You can change the settings during a run The respec tive altered setting flashes for a couple of seconds Once the display changes to the continuous mode the new settings become operative Stopping the centrifuge Stopping with preset time Normally the run time has been preselected and all you have to do is wait until the centrifuge terminates the run automatically As soon as the speed is down to zero the display reads End By pressing the open lid key IA you can now open the lid and remove your samples You can stop the centrifuge at any time by pressing the stop key Stopping with continuous operation If you have chosen continuous operation you must stop the centrifuge manually Press the stop key IE in the control panel The centrifuge starts braking at once and stops within a few seconds The displ2. tron s compliance with these Directives the recyclers in your country and information on Thermo Electron products wich may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROoHS 31 Operation for your notes 32 Maintenance and care Maintenance to be performed by the customer For the protection of persons environment and mate rial you are obliged to clean the centrifuge regularly and to disinfect it if necessary Unsuitable cleaning agents or disin fection procedures may damage the centrifuge and its accessories If you intend to use cleaning agents or disinfection procedures not rec ommended by the manufacturer you have to make sure by consult ing the manufacturer that the pro cedure foreseen does not cause any damages to the instrument Maintenance and care Cleaning er Clean the casing the rotor chamber the rotor and the accessories regularly and in case of need This is indi cated both for reasons of hygiene and to prevent cor rosion due to contamination sticking to the instrument and its accessories Clean them with mild agents of pH values ranging from 6 to 8 Immediately after cleaning dry the aluminum parts or put them into a warm air dryer at a temperature not ex ceeding 50 C Pull mains plug before cleaning the instrument During cleaning liquids and espe cially organic solvents should not come into contact wi
3. separate the rotor from the drive without opening the lid In this case you can open rotor upon removal from the centrifuge using e g a safety work bench before decontaminating it Danger of irreparable motor damage Never tilt the rotor Always grab it in the middle and pull out perpendicu larly 1 Open the lid of the centrifuge 2 Screw the cap nut open by turning it counterclock wise using the socket wrench delivered with the in strument Remove the cap nut 3 Grab the rotor in the middle and pull gently upwards off the drive shaft Be careful not to jam it 22 Loading the rotor Maximum loading Overloading may cause the rotor to explode Exploding parts may se verely damage the centrifuge Never exceed the maximum permis sible load of 4 g per place The Biofuge pico can reach high rotational speeds im plying enormous centrifugal force The rotors are de signed in a way warranting sufficient residual strength even at the highest permissible speed However this safety system presupposes that the maximum permissible load of the rotor is not ex ceeded If you wish to centrifuge samples that together with the adapters exceed the maximum permissible load you must either reduce the sample volume or calculate the permissible speed Nperm according to the following for mula maximum permissible load max n perm actual load Filling the centrifuge tubes Check carefully whet
4. 1 1 1 13 Before USC iniii rer anelser 15 Where to install the centrifuge u dsseneeeere 15 Mains connection ssssseereeerrrre reen 15 Removing the transport protection u sseere 15 Operation rr reagerede 17 Transport and installation 17 Mains connection c cceececeeeeeeeeeeeeeeeeeeeeeeneees 17 Contents Opening theld yinki arrastan senan aria 18 Emergency lid release 00 0 ceeeeeeeeeseeeeeeeeteeeeeeee 18 INS Mting the POtOM 22 2 ee eeeecescceceeeeeceeeeeeeceeeeeeeeeeeenees 20 Permissible rotor temperature 0ceeeeee 21 Lifetime of the rotor 0 cceeseeeeeeeeeeeeeeteeeeeeees 21 Removing the rolo lernetis enS AREEN KREST 22 Loading the rotor asne ki aean atian ianiai aahit 22 Maximum loading succeer klerk 22 Filling the centrifuge tubes u u u u ddsseecererererrnrer 23 Aerosol tight application 24 Checking for aerosol tightness 25 Placing the tubes in the rotor cceeeeeeeeeee 26 Selecting the SPC d ccceeseeeseeeeeeeeeeeeeeeeeeees 27 Selecting the run time u u u sesesereer nerne renee 27 Preselected run time ancir 27 Continuous operation 1 u ssseeerer erne nnnee 28 Starting the centrifuge Xu u u ssseneeeerrer renterne 28 Changing the settings during the run 1 1 1 1 1 1 28 Stopping the centrifuge W Xu u u u ddsenerererrer rer rnen renee 29 Stopping with preset time u sssceeerrrnnee 29 Sto
5. is again actuated call Service The lid is not properly closed Press the lid forcefully down on the right hand front part B Aw The excess temperature safety circuit of the motor has been actuated Pull mains plug Control ventilation slots underneath the instrument and clean if necessary After 20 min you can start the instrument again If the safety circuit is again actuated call Service 42 Troubleshooting 43 Troubleshooting In case you must call the Service Should you require our Service please tell us the order no and serial number of the instrument You find the pertinent information at the back of the instrument near the socket for the mains plug Moreover it is helpful for our service technician to know the valid software version You can determine the soft ware version as follows 1 Switch the instrument off 2 Switch the instrument on The display reads 888 88 for a couple of seconds Subsequently the display may read e g the follow ing readings for 2 seconds each 44 Software number OW 8 Software version dl NV RAM number 104 L NV RAM version 01 The values shown above are just examples Your read ings may be different In the example shown here the values mean the following e Software 0478 version 01 e NV Ram 1041 version 01 Troubleshooting for your notes 45 Technical data Technical data Component parts and performance Sheet steel with fitted p
6. keys The digit after the decimal point flashes for a number of seconds then changes to permanent display The speed is now stored Operation Selecting the run time You can select a run time between 1 and 99 min or continuous operation Preselected run time To predetermine the run time proceed as follows 1 Press one of the set keys l for an increase or I for a decrease in the time section of the con trol panel see also the foldout page in the cover 15 L By pressing the selected key briefly you increase or de crease the preset run time in steps of 1 min This option is supposed to be used for small changes and fine tuning If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler ated pace Release the key as soon as you have reached the desired value and fine tune if necessary by repeat edly pressing one of the keys The minute display flashes for a number of seconds then changes to permanent The run time is now stored 27 Operation Continuous operation To operate the Biofuge pico in the continuous mode you must press the key M until the display changes to hd for hold With this setting the centrifuge keeps running until stopped manually E Please note that the lifetime of plastic tubes in particular is limited Extended use may damage them 28 Starting the centrifuge
7. lid lock mechanism 7 lid open during run warning 7 lid opening 18 lid release emergency 7 mains connection 15 fuses 49 mains switch 17 maintenance 33 manual lid release 18 manual stop 29 Index maximum permissible load formula for 22 maximum sample density 3 mechanical lid release 18 O opening the lid 18 operation continuous 29 preselected run time 27 short time 29 organic solvents not allowed for cleaning 33 overloading dangers implied 22 P partial loading of rotor 26 pathogenic microorganisms protection against 4 permissible speed 22 power on 17 power supply 15 problems 55 Index handling of 39 protective tubes for corrosive substances 4 Q quick run function 29 quick run key 8 R radius of centrifugation for calculation of RCF value 30 RCF value 30 readings of control panel during run 8 relative centrifugal force 30 rotor cap nut for fixing 9 loading 26 partial loading 26 removal 22 rotor cap 21 rotor insertion temperature 20 run time continuous operation 28 56 fine tuning 27 range 27 setting 27 RZB Wert 12 S safety instructions 3 safety measures 3 safety standards 5 safety systems built in 7 safety zone 3 30 cm around centrifuge 15 sample density maximum 3 service 37 service contracts 37 set keys 8 setting runtime 27 short time operation 29 socket wrench 9 software check internal 17 software version determination 43 speed fine tu
8. mil PP maximum RCF value at Nmax 12 846 11 524 maximum radius cm 6 8 6 1 minimum radius om acceleration deceleration time s aerosol tight yes reduced filling permissible temperature range 4 C to 40 C 4 C to 40 C autoclavable number of cycles 121 C 10 cycles 121 C 10 cycles The values relate to vessel places 4 and 5 in the PCR Rotor 12 Adapters for rotor order no 7500 3328 Accessories Adapter Dimensions Capacity Number per Color Order No x H Set reduction sleeve PCR 6 2 x 20 mm 0 2 ml 24 gray 7600 3750 reduction sleeve 8 x 43 5 mm 0 5 0 6 ml 24 turquoise 7600 3758 reduction sleeve 6 x 46 mm 0 25 0 4 ml 24 red 7600 3759 Accessories for your notes 14 Before use Where to install the centrifuge The centrifuge must be operated in a place meeting the following criteria e A safety zone of 30 cm around the centrifuge must be maintained Hazardous materials must not be kept within this zone during centrifugation e The substructure must be stable and resonance free A good support is provided by a plane labora tory bench or a large laboratory carriage with cast ers that may be locked e To ensure sufficient air circulation a minimum dis tance from the wall of 10cm at the back and of 15 cm on each side must be kept e The centrifuge must be protected from heat and di rect sunshine e The location should be well ve
9. of the centrifuge Possible cause s and measures to be taken Lid cannot be opened Pressing the open lid key has no effect A 1 2 3 B Lid not correctly engaged or lid warped Check whether the mains supply is OK and the instrument is switched on displays lit Press the lid down on the right hand front end and actuate the open lid key once again In case these measures have not the desired effect you may open the lid with the emergency lid release see page 18 Heat monitoring relays in the lid unlocking magnets ha ve been actuated Press the key again after waiting for about 1 min Centrifuge is exceptionally noisy AON Stop the centrifuge by pressing the stop key I in case of emergency pull mains plug Wait until the centrifuge stands still Check whether the rotor is properly loaded Check whether a broken vessel damage to the rotor or mo tor malfunction was responsible for the noise If you cannot locate and solve the problem call Service br Instruments stops without brak ing There was a short mains failure or the instrument was switched off during the run Wait for about 75 seconds the instrument is then again ready for use 40 Troubleshooting Error Behavior of the centrifuge Possible cause s and measures to be taken E 0 Motor does not start Motor or rotor blocked 1 Switch the instrument of
10. or totally removed Changes in mechanical or electrical components of the centrifuge may only be carried out by individuals authorized by Thermo Electron For your safety Conformity to current standards Safety instructions in this manual Heraeus centrifuges are manufactured and tested according to the following standards and regulations CE for all voltages e IEC 1010 1 EN 61010 1 e IEC 1010 2 EN 61010 2 020 Pollution degree 2 Overvoltage category Il for 120 V only aan e CAN CSA C22 2 No 1010 1 92 e CAN CSA C22 2 No 1010 2 020 94 This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in its immediate sur roundings General hints are marked with this symbol IP Before switching on the centrifuge please read this manual For your safety for your notes The Biofuge pico The figure below shows the Biofuge pico with the lid opened and the rotor put in place The Biofuge pico Safety systems The Biofuge pico is equipped with a number of safety systems Connection rotor motor Body and rotor chamber made from impact resistant plastic Lid with hinge and control window Lid lock mechanism You can open the lid only when the power is turned on and the rotor has come to a halt You can start the centrifuge only if the lid is properly locked Warning if lid is manually opened during a run sa fety circuit actuated If
11. shape and surface Exchange faulty parts immediately Spare sealing rings 75003268 Checking for aerosol tightness The checking of the rotor type and bucket was done according to the dynamic microbiological test proce dure with regard to EN 61010 2 020 appendix AA The aerosol tight bio containment of the rotor mainly depends on proper handling A Check the aerosol tight bio containment of your rotor whenever necessary It is very important that all the seals and seal surfaces are carefully inspected for wear and damages like cracks scratches and embrittlements Operation For a quick test one can check the aerosol tight buck ets and fixed angle rotors according to the following procedure e Slightly grease all seals e Fill the bucket or rotor with approx 50 ml carbon dioxide mineral water e Close the bucket or rotor according to the respec tive handling instructions e Shaking the bucket releases the carbon dioxide of the water and an excessive pressure is built up e Leaks are recognized by humidity release and au dible disinflation of gas mix e Finally buckets respectively rotor lid and lid seal have to be dried 25 Operation Placing the tubes in the rotor The rotor must be loaded symmetrically When loading the rotor only partially you must ensure that opposite bores always receive tubes of equal weight when cen trifuging a single sample place a centrifuge tube e g filled with
12. the lid is manually opened during a run or if the temperature of the drive exceeds a critical value a corresponding message appears in the dis play Lid and OP respectively Emergency lid release only in case of emergency In order to permit you to remove samples even after a power failure the centrifuge is equipped with an emergency lid release see chapter Operation Ee Do not tamper with the safety systems The Biofuge pico Properties The Biofuge pico is a benchtop centrifuge for use in the biochemical and medical laboratory The preset speed is reached in seconds You can also spin samples for only a few seconds using the quick run key 63 if this is required for the task in question The extremely long lived maintenance free induction motor provides quiet and vibration free operation even at high speeds The user friendly Easycontrol control panel permits easy operation With the centrifuge turned on and the lid closed the preset speed and run time are displayed before the run During operation the control panel shows the actual values upon briefly pressing any one of the set keys El or M the preset values for speed and run time are displayed instead After the run the speed panel displays End If you press the amp or M keys repeatedly you increase the corresponding preset value stepwise If you press and hold down the chosen key the respective value increases continuously at first slowly an
13. 58 2711 North America Thermo Electron Corporation Phone 1 800 879 7767 275 Aiken Road Asheville NC 28804 USA Fax 1 852 2711 3858 E Mail info labequipment thermo com 275 Aiken Road Asheville NC 28804 USA Fax 1 828 645 9466 E Mail info labequipment thermo com 275 Aiken Road Asheville NC 28804 USA Fax 1 828 658 0363 E Mail info labequipment thermo com Internet http www thermo com Thermo ELECTRON CORPORATION Thermo Electron Corporation Robert Bosch StraBe 1 D 63505 Langenselbold Phone 49 0 61 84 90 60 00 Fax 49 0 61 84 90 70 00 In the interest of continuous product development we reserve the right to make changes without express notice 20057861 Pico NEU_uk 11 05 Printed in Germany
14. 7 cm end of thin wire e g a bent up paper clip or another suitable tool through the hole on the Operation upper right of the front panel see figure Push the lock bolt back until the lid unlocks audibly Remove the auxiliary tool and open the lid 4 In case the rotor still turns close lid immediately and wait until it has come to a complete stop Emergency lid release Never brake the rotor using your hands or tools 5 As soon as the rotor stands still remove your sam ples and close the lid Operation Inserting the rotor Improper or improperly combined x accessories may cause severe dam age to the centrifuge Rotors which are allowed for use in a Biofuge pico cen trifuge are detailed in the chapter Accessories and only these rotors are to be used in this centrifuge To insert the rotor you will need the cap nut and the socket wrench delivered with the centrifuge see the chapter The Biofuge pico Pieces delivered Possible damage to drive and rotor You may insert the rotor only if the temperature of the drive the rotor and the cap nut is between 10 C and 30 C 20 Proceed as follows 1 Open the lid and make sure that the rotor chamber and the rotor are clean Remove eventual dust for eign material or sample residues The thread and the O Ring on the motor shaft must be in perfect condition Turn the rotor so that the notch fo
15. Heraeus Biofuge peer Instructions for Use Thermo ELECTRON CORPORATION How to use this manual Use this manual to get acquainted with your AR centrifuge and its accessories The manual helps you to avoid inappropriate handling Make sure to keep it always close to the centrifuge A manual that is not kept handy cannot provide protection against improper handling and thus against damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessories Transportation and hook up Use of the centrifuge Maintenance and care Troubleshooting Technical data Index Overleaf you will find a graphic representation of the control panel with a survey of the most important functions Please fold out speed time quick run rotor turns Fy open lid stop start set keys back panel Before switching on the centrifuge socket for mains connection please read this manual mains switch The control panel of the Biofuge pico Display Speed Resting state preselected speed During run current speed rotating light rotor turns End End Running resting error codes if present Time Resting end preselected run time in minutes in hold mode hd During run remaining run time or with quick start run time passed Lid open OP Keys Start normal start Stop manual stop Open lid open lid p
16. ay reads End the electrical lid unlocking mechanism is avail able You can now open the lid by pressing the open lid key IV and remove your samples Operation Short time centrifugation For short term operation the Biofuge pico is equipped with a quick run function Short term centrifugation is started by pressing the quick run key L amp continuously it stops as soon as the key is released In this mode the centrifuge accelerates with full power up to the maximum speed of 13 000 rpm unless you release the quick run key The preset speed is ig nored The centrifuge accelerates to the maximum speed of 13000 min eo Check carefully whether you have to maintain a specific speed for your application During acceleration the time is counted forward in sec onds After 60 seconds the display changes to the min ute mode 29 Operation RCF value The relative centrifugal force RCF is usually given in multiples of the earth gravity g It is a dimensionless number that allows one to compare the efficiency of separation or sedimentation of diverse instruments since it is independent of the instrument used The only values entered in the equation are radius and speed of centrifugation 2 RCF 1118 r 1000 r radius of centrifugation in cm n speed in rpm At a speed of 13000 min the cen trifuge achieves a maximum per formance o
17. care Warranty conditions The warranty period starts with the day of delivery Within the warranty period the centrifuge is repaired or replaced free of cost if there are provable faults in ma terials or workmanship Conditions for a warranty are e the centrifuge is used according to the instructions of use e mounting extensions settings alterations or re pairs are carried out exclusively by personnel au thorized by Thermo Electron e the required maintenance and care procedures are carried out regularly 37 Maintenance and care for your notes 38 Troubleshooting Problems you can handle yourself Troubleshooting FE If problems other than those described in the following tables arise you must consult your nearest authorized service Error Behavior of the centrifuge Possible cause s and measures to be taken Displays remain dark The motor stops The rotor stops without braking The lid cannot be opened Mains failure or not connected 1 Is the mains switch turned on 2 Check the mains connection 3 If the mains connection is OK call your nearest Service Displays fail briefly The motor stops suddenly The rotor stops without braking The display reads br see br Brief interruption of mains supply 1 Check whether the plug is plugged in properly 2 Wait for 75 seconds 3 Restart the centrifuge 39 Troubleshooting Error Behavior
18. d after a few seconds at an accelerated pace You can change the preset values during operation Quick run operation As long as the quick run key I is pressed the ro tor is accelerated with maximum power potentially up to the maximum speed overriding any preset speed value Pieces delivered The Biofuge pico is delivered complete with a special cap nut for fixing the rotor 10 mm tubular socket wrench for fastening the cap nut fixed angle rotor 24 x 1 5 2 ml 7500 3328 cable for mains connection this Manual cap nut order no 70056208 The Biofuge pico tubular socket wrench order no 2036 0072 The Biofuge pico for your notes 10 Accessories The Biofuge pico is delivered complete with a fixed angle rotor with 24 holes for placing microliter tubes with a volume of 1 5 or 2 0 ml In addition you may order three sets of adapters con taining 24 reduction sleeves each With these adapters you can centrifuge all commercially available microliter tubes with a volume between 0 2 and 0 6 ml as well as 0 2 ml PCR reaction vessels A further option is a PCR Strip rotor Please consult our sales documentation for a complete collection of accessories including technical data order numbers and special low cost package offers For more information you can visit our web site at http www Thermo com Accessories Accessories Rotor program 24 x2
19. elow the speed display e runtime adjustable in steps of 100 min in the range of 2000 min to 13000 min adjustable in minutes from 1 min to 99 min hd mode continuous opera tion between 1 s and 60 s in seconds over 1 min in minutes 49 Technical data Function Performance Parameter memory e speed e time Function Performance Diagnostic messages e lid not properly closed display OP e general malfunction ERROR Codes see page 39 Testing standards 230V instruments Manufactured and tested in accordance with EN 61 010 1 EN 61 010 2 020 EN 61326 EN 61000 3 2 A14 2000 6 EN 55011 B 120 100V instruments Manufactured and tested in accordance with IEC 61010 1 1990 amendment 1 1992 amendment 2 1995 IEC 61010 2 020 1993 amendment 1 1996 CAN CSA C22 2 No 1010 1 92 CAN CSA C22 2 No 1010 1 B97 amendment 2 50 Electrical connections fuses Technical data Order no Voltage Frequency Nominal current Power consumption Fuses inside instrument 7500 3235 230 V 50 60 Hz 1 1A 150 W 2 x 2 A slow blow 5 x 20 mm 7500 3236 110 120 V 60 Hz 2A 150 W 2x 4A slow blow 6 3 x 32 mm The fuse may be replaced only by authorized servicing personnel 51 Technical data for your notes 52 Index A acceleration 8 accessories cap nut 9 rotor 11 tubular socket wrench 9 aerosol tightness test 25 aluminum rotor 35 autoclaving 36 autoclavi
20. f 16060 g Check carefully whether your tubes are designed for this centrifugal force and reduce the speed if nec essary 30 The figure for the maximum RCF value is based on the maximum radius of the tube oe You may take this into account when calculating the RCF value for your application The figure on the last page of this manual gives a graphic representation of the relation between speed and RCF Apart from the maximum RCF value RCF max lower li ne this graph also shows the minimum RCF value RCF min calculated for the meniscus of the sample up per line Please note that this value becomes lower depending on the tubes and adapters used Turning off the centrifuge The centrifuge is turned off by switching the main switch into the O position es The main power switch should be turned off after a complete centrifugation run Without motor deceleration it takes much longer un til the rotor comes to a halt The centrifuge lid can only be opened automatically if the centrifuge is turned on Operation WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the fol lowing symbol Thermo Electron has contracted with one or more re cycling disposal companies in each EU Member State and this product should be disposed of or recycled through them Further information on Thermo Elec
21. f and on again with the mains switch 2 Open the lid 3 Check whether the transport protection has been removed If you cannot solve the problem call nearest Service E 8 Overvoltage or over current at Mains voltage outside tolerance Brake resistance defective the U F converter Switch the instrument off and on again If the problem persists call Service E 10 Wrong check sum in the NV Switch the instrument off and on again If the problem persists RAM call Service E 11 Error in data transfer from NV Switch the instrument off and on again If the problem persists RAM call Service 41 Troubleshooting Error Behavior of the centrifuge Possible cause s and measures to be taken Message Lid appears in the display Display OP appears al though lid is closed Motor stops Rotor comes to a stop without braking Start impossible A 1 2 B e PO A The lid was manually opened during the run Press the lid shut The instrument comes to a stop without braking If you want to continue the centrifugation you must switch the instrument off and on again The message br is dis played and the centrifuge brakes see br The excess temperature safety circuit of the motor has been actuated Pull mains plug Control ventilation slots underneath the instrument and clean if necessary After 20 min you can start the instrument again If the safety circuit
22. ge tubes and adapters and dis infect them or dispose of them as necessary Treat the rotor and the rotor lid according to the in structions given for the disinfectant soaking in liq uid or spraying You must strictly observe the specified action times Turn the rotor head down and drain off the disinfec tant Thereafter thoroughly rinse rotor and lid with water Dispose of the disinfectant according to valid regu lations Aluminum rotors have to be treated with anticorro sive protective oil subsequently Maintenance and care Disinfection with bleaching lye These agents contain highly aggres sive hypochlorites and must not be used with aluminum rotors The following precautionary measures are to be taken for extensive protection of the 75003327 and 7500 3328 rotors 1 Avoid high temperatures The bleaching solution and the rotor should not be warmer than ca 25 C Do not let the bleaching solution act longer than ab solutely necessary After disinfection rinse the rotor thoroughly with dis tilled water and allow to dry 35 Maintenance and care Decontamination For general radioactive decontamination use a solu tion of equal parts of 70 ethanol 10 SDS and wa ter Follow this with ethanol rinses then de ionized wa ter rinses and dry with a soft absorbent cloth je Dispose of all washing solutions in appro priate radioactive waste container
23. her your sam ple vessels are permissible for the respective g value and reduce the speed if necessary The smaller the unbalance of the centrifuge the better the separation since separated zones are no longer perturbed by vibration It is therefore important to bal ance the centrifuge tubes as well as possible To minimize unbalance you should fill the tubes as evenly as possible You can achieve this by eye How ever you must nonetheless ensure that opposite tubes are filled to the same level Operation Please note that plastic sample vessels only have a limited service life particularly when used at maximum rpm or temperature and must be replaced as necessary 23 Operation Aerosol tight application not with open container lids The following steps have to be carried out 24 Lubricate the seals before inserting them lubricant order no 75003500 Insert the seal C profile in the groove at the side of the body of the rotor Insert the O ring into the inner groove on the screw on top Attention Please check that your sample containers are suitable for the centrifugal application desired 16060 x g temperature in uncooled devices approx 10 K above room temperature Please observe the permissible filling volumes Nominal volume Permissible volume 2 0 ml 1 5 ml 1 5 ml 1 0 ml others 7 nominal volume The sealing elements are to be checked regularly for damage to the
24. lastic chassis Keys and display panel covered with easy care protective foil Easycontrol system Dimensions diameter x height 188 mm x 63 mm Rotor chamber Up to 48 ml of spilled liquids are retained in the rotor chamber and cannot enter the instrument Lid lock Automatic locking when the lid is pressed shut Lid opening Electromagnetic release via the open lid key A when connected to mains Emergency lid release Lid release in case of power failure emergency opening with auxiliary tool 46 Technical data Function parameter Value environmental conditions indoor use max elevation 2000 m above sea level max relative humidity 80 up to 31 C linearly decreasing down to 50 relative humidity at 40 C Permissible temperature of the environment Maximum kinetic energy 1 65 kNm Permissible temperature of the rotor 4 C to 40 C Maximum sample temperature after 30 min room temperature 15 K run time Noise 56 dB A at maximum speed Dimensions H x W x D 206 mm x 233 mm x 335 mm Weight without rotor 9 8 kg 47 Technical data 48 Technical data The Easycontrol user interface Function Performance Quick run key short term run when pressed permanently stop when released is rotor is signalled by rotating light LED display in the speed pa nel The speed display reads end Number of cycles is displayed upon pressing the two keys DA b
25. ned personnel e their design is improperly changed e the safety instructions are not followed Therefore personnel involved with operation and maintenance of the centrifuge must read and fol low the safety instructions In addition the pertinent regulations for prevention of accidents must be strictly followed ee A This manual is an integral part of the centri fuge assembly and must be kept close at hand at all times When damages to the power cord or at casing are noticed the centrifuge must to be set out of operation For your safety Proper use The centrifuge is designed to separate liquid suspended materials having different densities and particle size respectively maximum sample density is 1 2 g cm ml at maximum speed Improper use During a run a safety zone of 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be present The centrifuge may cause harm to its user or other per sons or may damage goods if safety measures are not followed Centrifuging hazardous materials e The centrifuge is neither made inert nor is it explo sion proof Therefore never use the centrifuge in an explosion prone environment e Do not centrifuge explosive or flammable sub stances The same holds for substances prone to react violently with each other For your safety Do not centrifuge toxic or radioactive substances or pathogenic microorganisms
26. ng cycle permissible maximum 36 C cap nut for fixing rotor 9 centrifuge tubes types 11 volume range 11 centrifuging cycles display 47 changing settings Index during run 28 cleaning 33 common glass or plastic centrifuge tubes 4 conditions of warranty 37 contamination necessary measures 34 continuous operation 29 control panel readings 8 corrosion 4 corrosive substances protective tubes for corrosive substances 4 cycle counter 47 D damage symbol for potential 5 dangerous chemicals 3 decontamination 34 disinfectant 34 disinfection 4 procedure 34 disinfection with bleaching lye 35 display during run 28 displays 53 Index brief failure 39 not lit 39 E EC Guidelines 5 electrical connections 49 emergency lid release 7 emergency release lid 7 error messages or 40 E 0 41 E 10 41 E 11 41 E 8 41 lid 42 OP with lid closed 42 F fine tuning run time 27 speed setting 27 first steps 15 fixed angle rotor 11 formula 54 maximum permissible load 22 frequency 15 fuses 49 H handling the centrifuge 4 hazardous substances 3 hazards symbols used for 5 hints symbol for 5 icons for denoting dangers and potential damage 5 infectious material precautions in case of tube breakage 34 Instructions for use delivered with instrument 9 items delivered 9 K key quick run 8 set 8 keys general operation 8 L lid blockage 40
27. ning 27 permissible 22 speed of centrifugation for calculation of RCF value 30 speed setting 27 stopping 29 substructure 15 symbols for hazards and dangers 5 T temperature of the environment permissible 46 temperature range for rotor permissible 46 test aerosol tightness 25 toxins protection against 4 transport precautions for 17 tube breakage with infectious material 34 Index tubes types 11 volume range 11 Turning the centrifuge off 31 U unbalance 23 V voltage 15 W warning lid open during run 7 warranty conditions 37 wear 4 57 Index 58 Autoclaving protocol Autoclaving protocol 59 Speed RCF diagrams for PCR Rotor 7500 3327 100000 13000 10000 speed rpm 1000 min 4 7 cm max 6 8 cm 10 100 1000 1000 100000 RCF x g 12846 60 Speed RCF diagrams for 24 x 2ml Rotor 7500 3328 100000 13000 10000 speed rpm 1000 10 100 1000 RCF x g I 10000 W 16060 min 5 9 cM fmax 8 5 cm 100000 61 62 International contacts Europe Middle East and Africa Thermo Electron Corporation Robert Bosch StraBe 1 D 63505 Langenselbold Phone 49 0 6184 906940 Fax 49 0 6184 907474 gt E Mail info labequipment de thermo com Asia Pacific Thermo Electron Corporation Phone 1 852 2711 3910 Latin America Thermo Electron Corporation Phone 1 828 6
28. ntilated Before use Mains connection Make sure that voltage and frequency correspond to the specifications on the instrument label Turn the mains switch on the back panel off press 0 only then connect the centrifuge with the mains supply using the power cord supplied with the instru ment Removing the transport protection Turn the instrument on The display panel shows for about 12 s the routine internal software check se quence Open the lid by pressing the open lid key and remove the transport protection for the rotor Check that the rotor moves freely by lightly turning it and make sure the rotor is tightly screwed on The Biofuge pico is now ready for use Before use for your notes 16 Operation Transport and installation Damage to the centrifuge by jolts or jerky placement Transport the centrifuge only in the upright position using the special box provided with the instrument and secure it properly Place the centrifuge carefully Before using the centrifuge make sure that the transport protection has been removed vo Mains connection Make sure that your mains voltage and frequency match the specifications on the instrument Turn off the mains switch on the back panel push down the 0 mark then connect the instrument to the mains sup ply Turn on the mains switch at the back of the centrifuge Operation type plate with voltage and frequency mains switch s
29. ocket for mains cable For a couple of seconds the following reading appears in the control panel This tells you that the in 068 S strument carries out an in ternal check of its software see table on page 44 After this check the display changes The values now shown in the display panel are the ones last used The following figure gives an example of possible read ings A detailed description of possible settings is given below Operation a In this example the pre 5 0 C set speed is 5 000 rpm and the preset run time is 10 min Opening the lid For normal electrical unlocking connect the centrifuge to the mains supply turn the mains switch on and push the open lid key IA 18 Emergency lid release In case of a power failure you cannot open the lid nor mally using the open lid key To permit unloading even in this case the centrifuge is equipped with a mechanical lid unlocking system However you may use this system only in case of emergency Rotor can spin at high speed Touching it may cause severe inju ries A Always wait for several minutes until the rotor has come to a complete stop Without power the brake does not function and braking takes much longer than normal Should it be necessary to open the lid manually pro ceed as follows using an appropriate tool 1 Make sure the rotor stands still control window 2 Unplug the mains plug 3 Push a
30. ossible only with mains switch ON Quick run short term acceleration as long as key is pressed with indication of run time pas sed Set keys stepwise increase decrease of preset values accelerated change when pres sed permanently Short pressing of any of the set keys switch from current to preset value 0 1 E 0 E 8 E 1 E 1 br Lid OP Error codes troubleshooting see chapter Troubleshooting motor blockage transport protection removed excess voltage internal error internal error power turned off during run or power failure lid turned loose or opened during run drive overheated with lid closed safety circuit triggered drive overheated Warnings can span several display panels Contents For your safety scener 3 Proper SO oieri RE IR SNE LEDENDE ERE SENERE SERBERE 3 Improper USE hace haath BEES ER SARAS PAAY 3 Centrifuging hazardous materials 3 Handling the centrifuge eseeeeeeeeeteeeeeseeteeeeees 4 Conformity to current standards 5 Safety instructions in this Manual 5 The Biofuge pico scenerne 7 Safety systems 00 cece ccseeeneeeceeeeeeeaeeeeaaeeseneeenaees 7 Properes cA sa err a T Aa a 8 Quick run operation ccecceceeeceeeceeeeeeeeeeteeeeeees 8 Pieces delivered ssssseeeer rer 9 Accessories 2252 eee 11 ROtOr program see dare HA EN ENERET 12 Adapters for rotor order no 7500 3328
31. pping with continuous operation 1 1 29 Short time centrifugation W u ssssereerrer rer nnnee 29 ROF valensen kkeR ER BERS seek EEEE KARLS AR Eb ARS 30 Turning off the centrifuge 1 u u u u dssneeeeerrer ennen 31 WEEE Compliance Hein isise da thie ce eseesgtocdevsaeeeettiens 31 Contents Maintenance and Care 010ee 33 Maintenance to be performed by the customer 33 Gleanings rra fuss rarere feber esate cae aa eal 33 DISIMOCUOM TE TE A TE A 34 Decontamination ecccceceeeceeeeeeeeeeteeeeeeees 36 AUtOCIAVING saaara aA A STER SEERE ENES SENE HEER SES 36 Check whether autoclaving is permitted 36 The Thermo Electron Service Offer 1 1 1 1 1 1 1 37 Warranty conditions cceeeeeeeereeeeteeeeteeteneeeeees 37 Troubleshooting ccccceeeeseseeeeeeteeeeeeeeees 39 Problems you can handle yourself 39 In case you must call the Service 44 Technical Gata 153553 46 Component parts and performance 1 1 46 The Easycontrol user interface 49 Electrical connections fuses sees 51 DON AEAEE E nwa EROS KURERE RESEN 53 Autoclaving Protocol 59 Speed RCF diagrams scener 60 For your safety Heraeus centrifuges are manufactured according to current technical standards and regulations Nonethe less centrifuges may pose danger to individuals and surrounding if e they are not used as designed e they are operated by untrai
32. r engaging the drive shaft points downward Place the rotor on top of the drive shaft so that the notch of the rotor is located precisely above the re taining pin Push the rotor gently down until the thread is com pletely laid bare see figure If you have placed the rotor correctly you can screw on the cap nut easily and secure it with the tubular socket wrench delivered with the instrument Place the rotor cap onto the rotor Do not push the rotor down using force If you cannot screw on the cap nut you must carefully lift off the rotor and insert it again Ee Regularly check the proper positioning of the rotor and retighten the cap nut as nee ded Operation Permissible rotor temperature The rotors are only to be used within the temperature range from 4 C to 40 C Pre cooling in the freezer is not permitted Lifetime of the rotor There is no limitation on the service life of the high per formance rotors However please observe the following due to safety reasons Rotors and accessories made of plastic should not be exposed to direct sunlight and UV rays If the rotor shows signs of discolor ation deformation or wear or is out of balance it must be exchanged straight away 21 Operation Removing the rotor To remove the rotor you must follow the steps de scribed above in reverse order With the hermetic lid you may in case of contamination
33. s Autoclaving Ee Check whether autoclaving is permitted You may autoclave the rotor and the adapters at 121 C Maximum permissible autoclaving cycle 20 min at 121 C Ee For safety reasons the 7500 3327 and 7500 3328 rotors must only be subjected to a maximum of 10 autoclavings 36 The rotor must be cleaned and rinsed with distilled wa ter before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic rotors on an even surface to avoid deformation Chemical additives to the steam are not permitted a Never exceed the maximum permis sible values for autoclaving tem perature and autoclaving time Should the rotor show signs of wear you must stop using it The Thermo Electron Service offer Thermo Electron recommends annual servicing of the centrifuge and the accessories by authorized customer service or trained professionals The customer service personnel is inspecting e the electrical installations e the suitability of the location e the lid lock mechanism and the safety circuit e the rotor e the rotor fastening and the drive shaft Defective material is exchanged Thermo Electron offers inspection and service con tracts covering it Inspection costs are charged as flat rate contracts Necessary repairs are carried out free of cost within the warranty conditions and against payment after ex piration of the warranty period Maintenance and
34. th the drive shaft and the ball bearing Organic solvents may decompose the lubricant of the motor bearing The drive shaft may block 33 Maintenance and care Disinfection If a centrifuge tube containing infectious material leaks during a run you have to disinfect the centrifuge im mediately Infectious material could enter the centri fuge if spills or tube breakage occur Danger of infection may occur upon con tact Take appropriate protective meas ures for personnel Mind the permissible filling volumes and loading limits for the tubes In case of contamination the operator has to make sure that no further persons are jeopardized Contaminated parts have to be decon taminated immediately If required further protective measures have to be initiated 34 Rotor and rotor chamber must be treated with a neu tral universal disinfectant Best suited for this purpose are disinfectant sprays ensuring that all rotor and ac cessory surfaces are covered evenly A e You may disinfect the rotor and the accessories as described in the following section Be sure to follow the pertinent safety procedures for handling infec tious material 1 Pull mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft Please note the safety measures and handling hints when applying these substances 4 Remove the centrifu
35. water The following figure gives examples for proper loading properly loaded rotors 26 Improper loading can in the worst case lead to damage to rotor and centrifuge Unbalance not only causes a noisy run but rapidly dam ages the motor suspension improperly loaded rotors When you have loaded the tubes fasten the rotor lid by screwing the cap nut centrally on it Close the lid of the centrifuge by firmly pressing it down There must be a clicking sound and the lid must be locked so that it cannot be opened manually Selecting the speed The centrifuge can be set to a minimum of 2000 min and to a maximum of 13 000 min The built in micro processor prevents higher or lower speed settings Be tween these extremes you can select the speed in steps of 100 rpm using the following procedure 1 Press one of the set keys LA for an increase or J for a decrease in the speed section of the control panel see also the foldout page in the co ver By pressing the selected key briefly you increase or de crease the speed in steps of 100 rpm This option is sup posed to be used for small changes and fine tuning If you keep the key pressed the display changes at first slowly and after a few seconds at an acceler ated pace Release the key as soon as you have reached the desired value and fine tune if necessary by repeat edly pressing one of the
36. without suitable safety systems If microbiological samples of risk group II according to Laboratory Bio safety Manual of WHO are be ing centrifuged aerosol tight bio seals have to be used For materials in a higher risk group more than one precaution is required Should toxins or pathogenic substances enter the centrifuge or its parts you must perform appropriate procedures for disinfection see Maintenance and care Disinfection Strongly corrosive substances that may cause dam age to materials and reduce the mechanical strength of the rotor may be centrifuged only inside protective tubes Handling the centrifuge Use only original accessories for the centrifuge The only exceptions are common glass or plastic centri fuge tubes if they are approved for the rotor speed and RCF values Never use the centrifuge unless the rotor is properly installed You may use the centrifuge only with a properly loaded rotor You must not overload the rotor Strictly follow the rules and regulations for cleaning and disinfection If the rotor or the rotor lid shows signs of corrosion or wear you must stop using it Never open the lid manually if the rotor is still turn ing You may use the emergency lid release only in case of emergency e g during an interruption of power supply see chapter Troubleshooting Never use the centrifuge with an opened lid Never use the centrifuge if the front panel has been partially
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