レジオネラ検出試薬キットE - Eiken Genome Site
Contents
1. Positive Contro 4 5 Leg PC Leg DNA gt 100 2 mL 4C 13 000xg 0 40L 1 960 L2. 7 25 xL 3mol L 500mol L LAMP LAMP 1 Legionella pneumophila 1 19 19 8 2 60 CFU Colony Forming UnibD test 3 egzoze 2 pneumophizla serogroup 11 2 egzoze
3. 3 7 8 Exp Da e 9 20 20C 1M 1 ris HCI p H7 0 Tris PP Sm Loopamp
4. 10 UV 4 5 6 HB
5. Extraction Solution for Legionella EX Leg 50L 95 15 1M Tris HCI pH 7 0 Tris 8L 4C 13 000xg 10 lt gt 1 Reaction Mix Leg RMLeg 20L Bst DNA Polymerase luL 21wL Ne gt 1 20L 5xL
6. 1 x3 a 1 x3 3 a DNA gt 1 100 2 mL 4C 1 960L 2 0 mL 13 000Xg 10 2 40L 2 Extraction Solution for Legzonella EX Leg 50L 3 95 15
7. 100 DNA DNA Polymerase gs DNA Po DNA Eiken GENOME SITECURL http Reaction Mix Leg RM Leg 5s7 ymerase oopamp eiken co jp 1
8. CE 2 80 20 Positive Control Leg PC Leg Positive Control Leg PC Leg 3 Bst DNA Polymerase
9. Extraction Solution for Legionella EX Leg LAMP 60 2 Positive Loopamp e 2 o 0 4 0 4 0 3 PP 02 0 2
10. 4 1M Tris HCI pH7 0 Tris 8L 1 2 5 4C 13 000xg 10 2 000 4 1 20L 2 54L 3 PC Leg 5L EX Leg 5L 2 1 Loopamp
11. Loopamp 4 4 5 1 Loopamp
12. 0 1 0 0 0 0 10 20 30 40 50 60 0 10 20 30 40 50 60 1 2 2 2 1 1 100 2mL 40L 1 960L 2 DNA 2 20
13. 0 5 10u 2 0 mL Loopamp 0 5 mL 1 5 mD L 10 100guL 100 1 000D 95 2 ooOo Loopamp LA 320C RT 160C LA 200F 1 20 2 1 1 Reaction Mix Leg RM Leg 20uL gs7 DNA Polymerase 1uL 2
14. E 48 20 C 1 LMP 661 1 No 2 Nagamine K et al C omi T et al Nucleic Acids Research 28 No 12 e63 2000 in Chem 47 No 9 1742 3 2001 23 E Mori Y et al Biochem Biophys Res Commun 289 No 1 2000 50 154 2001 5 26 E 2003 2000 13 No 1 19 25 2003 JARMAM 14 No 1 25 30 2003 9 Beige J et al J Clin Microbiol 33 90 95 1995 10 11 32 No 54 No 3 667 715 1999 012
15. Po 5pL LAMP 25D sitive Control Leg PC Leg 5uL Extraction Solution for Legionella EX Leg LAMP gt 65C 60 80C 2 3 1 Loopamp Loopamp g 2 3
16. 65 75 60 80 C 2 25L Positive Control Leg Extraction Solution for Legionella LA 320C RT 160C LA 200F Eiken GENOME SITE URL http Iloopamp eiken co jp 2 3 65 4 Positive Control Leg PC Leg 1 5 6 Control Leg PC Leg 80 2 7
17. 2 Loopamp 20 3 Positive Control Leg PC Leg 20 6 1 Ne 2
18. serogroup 11 21 Loopamp LA 3 200F LAMP 20C RT 160C LA 6 48 1 Extraction Solution for Legionella EX Leg 1 8 mLX2tubes 2 1M Tris HCI pH7 0 Tris 1 0 mLX 1 tube a 3 Reaction Mix Leg RM Leg 1 0 mLX 1 tube 4 Bst DNA Polymerase Bst DNA Polymerase 1 60LX 1 tube 5 Positive Control Leg PC Leg 0 1 mLX 1 tube 1 LAMP
19. 0 2 2 308 421 0 481 487 2004 3LP6619 E
20. 3LP6619 E 201 200 A Loopamp LAMP Loop mediated lsothermal Amplification 0 9 5 8 1 4 E LAMP Loop mediated lsothermal Amplification 6 4 Primer LAMP 3 5 1 16S rRNA LAMP Legionella pneumophila 11
21. 77g pneumophila 3 100 49 LAMP 51 LAMP 22 1 1 LAMP 3 UV 2 DNA
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