取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. 4 Ligation 2 1 DNA TE DNA e DNA H M L TA DNA eTaq PCR PCR KOD 72 C 2min KOD Taq Unit dNTPs 0 2mM 10XBlunting Buffer TE 10mM Tris HCI 1mM EDTA pH8 0 3 2 DNA sDNA 10pmol pUC18 DNA2 7kb 10ug DNA 8ul 3 e KOD dNTPs2. Blunt end Ligation Ligation high 1 Ligation Kit Ligation 4 Control DNA Ligation 1 2 20 20HI KOD DNA Polymerase 2 5U ul 100ul 10XBlunting Buffer 375ul Ligation high lt 50ul Control DNA 10ng pl 10XBlunting Buffer 1 2M Tris HCI pH8 0 at 25 C 100mM KCI 15mM MgClz 60mM NH4 zSO4 2mM dNTPs 1 TritonX 100 0 01 BSA Ligation high DNA Ligation kit Ligation high LGK 101 Control DNA Eco I Sph 1 double digest pUC18 DNA Ligation plasmid DNA 2 3 1 1 Sample Control pl pl Sample DNA X Control DNA 10ng pl 5 10XBlunting Buffer 1 10XBlunting Buffer 1 KOD 2 5U pl 1 KOD 2 5U pl 1 8 X 3 10 10 2 72 2min 3
3. KOD 3 5 exonuclease DNA 4 e72C 2min 30min 3 Ligation 1 Sample Control ul phD Sample DNA Y Control DNA 2 5ng pl TE 10 Y TE 8 Ligation high 10 Ligation high 10 20 20 2 16 1hr 3 Control eLigation DNA co e LB 37 C overnight Ligation Control DNA Control DNA Ligation cfu colony formation unit Ligation 20 4 4 Liqation 1 Ligation high e 20 C
4. elLigation high 5 10min eDNA Ligation high es16 C e 2 eLigation 16 KOD KOD eControl Ligation 16 1hr eLigation high 16 C 30min Ligation 1hr es DNA Ligation overnight TE 5 4
5. 1 2 Ligation CH DNA X 10XBlunting Buffer 1 KOD 2 5U ul 1 8 X 10 72C 2min pl DNA Y TE 10 Y Ligation high 10 20 16 C 1hr 6 5 Code No KOD DNA Polymerase KOD 101 Ligationhigh LGK 101 T4DNAPolymerase TPL 101 Klenow Fragment PLA 111 T4DNALigase LGA 111 T4 Polynucleotide Kinase PNK 111 Alkaline Phosphatase E col BAP 111 Alkaline Phosphatase Calfintestine CAP 101 Competent high co7JM109 DNA 900 Competent high EcolDH5 DNA 901 Competent high Eco7DH5g DNA 903 7 HPCR 1 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order lifescience toyobo jp
6. TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
7. ldeas amp Chemistry Blunting and Ligation Kit Blunting high Code No BLK 101 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3269K Ligation Ligation lt 1 Blunting high DNA Ligation 1 KOD DNA Polymerase KOD 7ococcus sp KOD1 DNA Polymerase 5 3 polymerase 3 5 exonuclease qdNTPs DNA 2 2min DNA 3
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