NanoDrop 3300 ver. 2.8 Users Manual 日本語 （3.7
Contents
1. 0 5 M HCI 5 uL 1 lt 2 1 2 5uL dH20 2 gL2. 3 1 SECTION 3 amp fE S fig
3. Technical Support Fax C IND 3300data gt Username gt Application Module 13 5 SECTION 14 RFE C TREE 14 NanoDrop amp NanoDrop 3300 Xx dH20 0 596 1 10
4. bank 1 Configuration Advanced Parameters User Preference Max Gain Ratio Adgvanced Parameters User Preference High Gain Ratio Notification The ratio ofthe blank gain to the sample gain has exceeded the Max Gain Ratio setting This could be due to a dirty sample pedestal dust or micro bubble in the sample or improper sample column formation It is recommended that you clean the pedestal and load a fresh aliquot of sample and make a new measurement Ifthis message is recurring frequently it is recommended that you increase the Max Gain Ratio setting accessible in User Preferences Please referto User s Manual for more guidance Disregard this measurement Accept this measurement Accept all gain ratios during
5. Low Blank Gain Notification blank gain blank LED Min Blank Gain 13 4 SECTION 13 Low Blank Gain Notification The gain value forthis blank is below the Minimum Blank Gain setting This could be due to a dirty sample pedestal dust or micro bubble in the sample or improper sample column formation It is recommended that you use a fresh aliquot of water and blank again Ifthis message is recurring itis recommended that you change the Minimum Blank Gain setting in User Preferences Please referto th
6. 1 USB 2 NanoDrop CD CD nd3300 v2 5 0 install exe Install Software 3 PC USB Found New Hardware Wizard Windows XP SP2 operationg system No not this time
7. 2008 Thermo Fisher Scientific Inc All rights reserved Microsoft Windows Windows NT Excel Microsoft Corporation Adobe Acrobat Adobe Systems Incorporated Thermo Fisher Scientific amp o NanoDrop Thermo Fisher Scientific 2008 3 HX pol 1 1 SO m m 1 1 A xbI d PPS cdi 1 1 c Mc 1 2 VE
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9. Standard e Standard Curve Data Point Delete Point o Interpolatton linear interpolation o Linear o 2nd or3rd order Polynomials Standard Curve Standard Curve Type OE 25000E 0 59000E 0 750 00E 0 Linear Polynomial 7 3 SECTION 7 BAJE E FI ZAEOM E File File 3 Edit Configuration Standard Curves Help View Standard Curve
10. SUS303 Thermo Fisher Scientific 1 14 1 15 e 1 2 uL e 3 20 LED e LED e UV 365 nm 470 nm 500 650 nm s 2048 CCD e HERRA 400 750 nm s 1nm s 8 nm FWHM at Hg 546 m es lt 5 CV 10 nM Fluorescein s 1 15 e DWH 20 cm X 15 cm x 12 cm e 3Kg ee 303 o 5 vdc USB 2 W s
11. Stop NanoDrop C CAND 3300 Data Can t Find LabView RunTime Engine NanoDrop 13 3 SECTION 13 EZUSB SYS Cannot Be Found OS Windows 2000 7
12. 7 1 SECTION 7 AJE E FI 2tEIOM E es Reset Window Reset Window e Method e Source Source es Measurement Type o Reference o Standard o Sample RFU relative fluorescent unit e Sample ID Sample ID
13. 4 2 SECTION 7 BAJE E FI AJUO EBE e Units Confirmation C Standards Reference Standard Confirm selected units Confirm or change units All Standards in a group must utilize the same units Units j nd Don t show this dialog again IF this box is checked the user preferences For Ehe current user will be changed sn that confirmations of selected units For standard curves are na longer utilized e Additional Standards Standard 1 1 Standard 1 Standard Measurement Type Sample Standards Units pM Reference and Standards 1 00E 0 0 Reference Standard 1 Standard 2 Delete Standard Reference Standard Standard
14. DMSO Main Menu Start 2 Programs gt NanoDrop gt NanoDrop ND 3300 NanoDrop 3300 2 7 Default User s Manual Nucleic Acid Protein Other Create Edit Fluorescence Quantitation Quantitation Fluorophores Method Profiler Data Source User Account Viewer Check Preferences Management e Nucleic Acid Quantitation 33258 Hoechst dye PicoGreen dye RiboGreen8 dye Quant iT DNA HS Quant iT DNA BR e Protein Quantitation Quant iT Protein Fluorescamine Fluoraldehyde OPA FluoroProfile e FITC Fluorescein LED Cy3 Alexa Fluor 555 Cy5 Alexa Fluor
15. Help Show Context Help Ctrl H Context Help Import Data Viewer Data Import Data Import Folder Directory Tree Import folder Sample Information RFU 3266 r 1 5 CAND 3300 Data Des 3 4 5 Hold down the Control or Shift keys while selecting files or samples in the directory tree to select either mulbple individual or a range of samples respectively Directory Tree 350 400 450 500 550 600 650 700 750 800 j PicoGreen dsDNA Wav nm n dsl If archive files do not appear in directory tree they will need to be converted Launch Archive File Converter software to make the c
16. 0 5 1 10 SUS303 1 2 0 2 2 uL ND 3300
17. SECTION 7 BAJE E FI ZAJUOM E e Show Advanced Parameters Show Advanced Parameters x COULTON si Eile Edit Configuration Standard Curves Help Re blank Print Screen Recording View Standard Curve Bienk Print Repot Show Repon Min Blank Gain 5 00 Make new BLANK Measurement Advanced Parameters e Min Blank Gain LED reference blank gain UVAE LED 5 UV LED 10 s Max Gain Ratio gain blank gain Max Gain Ratio
18. Reference Spectrum LED virtual emission filter interval AA LED virtual emission filter interval AA nm virtual emission filter interval 0 1 1 SECTION 1 H initial Reference Spectrum Total Fluorescence TU Normalized Reference Spectrum Sample Fluorescence off angle LED
19. FITC FITC LED FITC Eile Edi Configuration SIandard Curves Help 1 ER e Re blank Print Screen Measure f uorescem FITC FAM Load 1 to 2 ul of your sample and click the measu 40 560 580 600 620 Wavelength nm 10 1 SECTION 10 FLUOROPHORES UVLED e DyLight 405 DyLight 405 400 nm 20 nm Alexa Fluor 405 e Quinine Sulfate Qunine Sulfate Quinine Sulfate 450 nm 20 nm e 4 Methyl Umbelliferone 4MU 4 methyl umbelliferone 450 nm 20 nm 4 methyl umbelliferoneno 4 MU B galactosidase B galactosidase 4 methyl umbelliferyl G D galactoside 4 methyl umbellif
20. LED virtual emission filter interval AAY AA 1 2 uL 1 mm LED 1 NanoDrop 3300 CAND 3300 Data PC USB Thermo Scientific NanoDr
21. Show Report NanoDrop 3300 PC Data Viewer Data Viewer Plots Reports Standard Curves 2 3 SECTION 11 RIFT 4 amp DATA VIEWER Show Repot Plots Show Report Data Viewer Start Report Recording Tool Bar 3
22. RFU 3 Cd e Saturation nm Saturation nm LED UV LED 25 nm LED 40 nm Saturation nm Saturation nm 1 Saturation nm Configuratton 2 Auto Gain
23. LED 555 nm Cy3 Alexa Fluor 555 Units method editor Create Edit Method Escape Key ESC Escape Escape 2 Exit Ctrl Q Exit
24. USBView 1 8 Technical Support 13 1 SECTION 13 Connection Errors Connection Error There was an error communicating with the instrument Try the following Check the USB cable and select Retry If Retry fails disconnectthe USB cable and then reconnect and select Retry again lf this does not solve the problem referto the Troubleshooting section ofthe User s Manual available from Main Menu USB Retry PC USB Retry
25. Save Report As Choose how to save the report Choose this option to be able to reload the Full Report repor into the Data Viewer at a later date Choose this option to export a tab deliminited Export Report text file of the displayed Report Table Table Only suitable for import into Excel Choose this option to export a tab deliminited Export Report amp text file ofthe displayed Report amp Standards Standards Tables Tables suitable for import into Excel Cancel Full Report Data Viewer Alk Load Report Ctrl L Load Report User Preference nfr 2 Excel
26. 3 Fixed Gain Saturation nm e Prompt Close Data Viewer Data Viewer Standard Curves Save As Ctrl A Load Ctrl L Eile Edit Configuration Eiee Ee ROTE Save s Ctri A Re blank Load Ctrl L Show Repon Show Context Help Ctrl H Help Show Context Help CTRLH Context Help File Edit Configuration Standard Curves BEAD User s Manual Ctrl M Re blank Show CantextHelp CH Show Repon 5 SECTION
27. dsDNA Quant iT dsDNA BR LED 530 20 nm 2ul Quant iT dsDNA Broad Assay 2pg Linear Range 4 1 ng ml 500 ng ml Quant i DNA H5 Eile Edit Configuration Stendard Curves Help Re blenk Print Screen Recording View Standard Curve User Default A Reset Window Blank PrnRepon ShowRspor Losyehditendaldsli 4 22 2003 2 16 PM Measurement Type QuentiT DNA HS Source Blue Open Protocol Sample Standards Load 1 to 2 ul of your sample and click the measure button Sample ID Sample 1 1610 0 1400 0 Dilution Factor 100 Z 1200 0 Sample 1000 0 800 0 x 600 0 400 0 Actual Gain 200 0 10 00 0 0 2700 i 6 i i RFU G530nm 14557 500 525 550 575 600 625 650 Wavelength nm ng ml 6 8 27 0F0013 Sybr Green Sybr amp Green dsDNA NanoDrop3300 dsDNA Sybr Green LED
28. Connection Error PC USB PC PC Cr PC USB Error Code 8 Error Code 8 Error reading a configuration file This is most likely caused when a user does not have write access to the folder containing the NanoDrop operating software usually found In CAprogram files Contact your PC administrator to share this folder with Read and Write permissions for all users For more details referto the Troubleshooting section of the User s Manual accessible below OPEN USERS MANUAL BEFORE EXIT EXIT 3 dye list log passwords log user preferences log Administrator Administrator program files folder C NND 33
29. d Create Save Method Fluorescamine Fluorescamine protein dye Fluorescamine NanoDrop3300 UV LED 365 nm 470 20 nm Fluorescamine protein assay 8 ug mL Linear Range 8 ug ml 500 ug ml Fluorescamine e Eile Edit Configuration Standard Curves Help Recording View Standard Curve User eto s MUT Reset Window Ext Re blenk Prints Blank PrntRepon ShowRepor Valid Standards 4 18 2008 11 41 AM Measure arius Measuremen t Type Open Protocol gt Sample Standards Meke new BLANK Measurement Fluorescamine BSA Dilution Factor 100 Z i i i i i i i RFU 470nm 4137 5 25 550 575 600 625 650 675 700 Wavelength nm ug ml 175 4 27 0 F013 FluoroProfile Fl
30. Welcome to the Found New Hardware Wizard If your hardware came wit or floppy disk insert it now Windows XP SP2 Windows NanoDrop 3300 MS Sans Serif font 8 s e e e OK
31. User Preference General Settings File Edit psa lullya Standard Curves Help View Standard Curve show Report y Silent Operation TM 4 Auto Scale Plot 7 77 Show Raw Plot Show Advanced Parameters Prompt Close Data Viewer mi e Silent Operation Configuration es Auto Scale Plot e Show Raw Plot RFU RFU Raw RFU Raw 2 Raw Raw RFU 2
32. NanoDrop 3300 LED UV LED max 365 nm 400 nm GFP wt Em509 465 nm 10 nm Quinine Sulfate Em450 Hoechst 33258 Em450 4 MU Em450 Q Dots various Blue LED max 470 nm 495 nm GFP wt Em509 EGFP Em509 470 nm 10 nm FITC FAM Em515 Alexa 488 Em520 PicoGreen Em525 RiboGreen Em525 Alexa 555 Em565 B Phycoerythrin Em575 Q Dots various White LED range 500 650 nm Cy3 Alexa 555 Em565 Alexa 568 Em600 Cy5 Alexa 647 Em667 Sulforhodamine 101 Em 600 dim MN 5 CMTREm570 460 nm 650nm Q Dots various TET Em535 HEX Em555 LED 460 650 nm LED LED LED LED
33. s Level 10 level 10 level 10 Administrator nanodrop level 10 level 5 Administrator 10 e Level 5 User Preference c NND 3300 data
34. blank low gain value Min Blank Gain 5 e Max Gain Ratio gain blank gain gain gain Max Gain Ratio Max Gain Ratio 3 Gain Notifications User Preference Save Preference 5 1 SECTION 5 USER PREFERENCES User Preferences User Default Cea General Settings Archiving Reports Duplicate Data Storage OFF Duplicate Data Fnlder g Not A Path gt Primary data storage is always enabled and located at CAND 3300 Data Archiving Data C1ND 3300 Data gt User
35. USB 7 DER File Options Help E My Computer Device Descriptor Intel r 32801DB DBM USB Universal Host Controller 24C2 bcdUSB 0x1020 RootHub bDeviceClass 0x00 bDeviceSubClass 0x00 Port1 NoDeviceConnected bDeviceProtoco 0x00 Port2 NoDeviceConnected ntel r 32801 DB DBM USB Universal Host Controller 24C4 RootHub Port1 NoDeviceConnected Port2 NoDeviceConnected lt ntel r 82801 DB DBM USB Universal Host Controller 24C7 5 RootHub bNHunConfigurations 0x01 Fort NoDeviceConnected ConnectionStatus DeviceConnected Port2 NoDeviceConnected Current Config Value 0x01 ntel r 32801DB DBM USB 2 0 Enhanced Host Controller 24D Device Bus Speed Full RootHub Device Address 0x03 Port1 NoDeviceConnected Open Pipes 4 Part2 NoDeviceConnected Endpoint Descriptor Port3 NoDeviceConnected bEndpoint ddress 0x02 Port4 NoDeviceConnected Transfer Type Bulk Port5 NoDeviceConnected wHaxPacketSize 0x004 4 PartB DeviceConnected Generic USB Hub kd Port1 NoDeviceConnected Endpoint Descriptor Port2 DeviceConnected NanoDrop USB Device i 0x00 Port3 NoDeviceConnected pe Control Port4 NoDeviceConnected wMaxPacketSize 0x0507 1287 Devices Connected 2 7
36. Sample ID Sample ID ID reports Data e Dilution Factor Measurement Type Mesurement Type m dilution factor 1 Sample RNA ID Dilution Factor sw Sample 31 Actual Gain A RFU Tr 535 5094 Dilution Factor 5 RFU 535 nm 509 4 ng ml 504 0 Measurement Type Sample Standards Sample RNA ID Dilution Factor 1 00 Sample 4 33 Actual Gain RFU 10 00 535 517 5 Dilution Factor 1 RFU 535 nm 517 5 ngiml 1034 e Sample e Measurement Type Standards Measurement Type T Standards Reference Standard 1
37. S5S7 g77 Prog g7s Mag7no op Utilities USB Reset USB Reset USB Reset Downloads 3 USB Reset Found New Hardware Windows XP SP2 operating system No not this time Found New Hardware Wizard Found New Hardware Wizard welcome to the Found New Hardware Wizard Windows dudes ids ent and updated s eue re by This wizard helps you install software for looking n the hardware installation CD o the Wir int D s Upda Hs Ps ris ite with your permissio n NanoDrop USB Devi Head our privacy polic i If your hai aeg pae Eh Can Windows connect to Windows Update to search for S software SE o
38. Help Start gt Programs gt NanoDrop gt ND 3300 version 3 2 SECTION 3 amp fE S fig User Preferences Source Check LED Duplicate data storage User Preferences 2 Account Management Account Management Account Management Aqdministrator level 10 Account 3
39. e Default Level 0 User Preference c NND 3300 data Default Administrator Default User Access Manager Bp xf Actions Modify User All Users ce Full Name Level Active Locked Expired Expires ES en Ie SEO Lunes EE r Administrator 10 Active Not Locked Mot Expired Never joel e
40. Cursor RFU X Y Reset Baseline x 0 Reports Reports Report SECTION 11 RIFT X amp DATA VIEWER Select the report columns to display and the order that the columns should appear Change Buffer Moda PromptenFulBufle Hac Sutter Sue the order by selecting and dragging a column header string to the desired position Sampi Uver Owe Trre Meewewet Gon Un Pat fa Report columns Sample ID User ID Date Reset Time Measure Type Gain Move Up Conc Units Move Down Ref nm v Cancel Report s Configure Report Ctrl F
41. Excel PicoGreen dsDNA 2006 10 10 nfd 2006 10 10 PicoGreenO 7 nfd Data Viewer Data Viewer Excel
42. USB Retry USB Sg po Lb itt o ETOC CERRO CRO dg Ton g oan amp D ME 15 5 30 B Bat AYIM D BL E fs BE wj 12 9 Cen ORIS 7 QU CCSLIRUT IRES CIE 20 9 0 hk 3 8 CIUS REAN EEH CEO MEPER RIY EHI PC USB
43. www nanodroD com USB 2 1 SECTION 2 2 2 SECTION 3 amp fE S fig 3 1 2 7 3
44. CAND 3300 Data Reports Report Method Date and time Report Name Report Mode Max Report size 200 11 4 SECTION 11 RIFT 4 amp DATA VIEWER Ignore Save Print Save and Print Show Report report Data Viewer Import Standards Standards Ele CcnWguiahcn Reports Heb Plots Report Standards Method Standards Sample Ret Ret Std 1 Std 1 Std 2 Std
45. 1 2 Measurement Type Measure Blank amp 7 IX Reblank Measure blank Blank bank Reblank L1 Microsoft Excel Fluorescein FITC FAM 2006 11 13 v2 6 nfd Read Only e File Edit View Insert Format Tools Data Window ACT Help Type a question for help B F18 M A B o H K L M N o 1 Method Fluorescein FITC FAM 2 Software 2 6 3 Sample ID UserID Date Time Measure T Gain Conc Units Min Gain Max gain r Dilution fac Norm coe Formula RFU nm1 4 Blan Default 11 13 2006 8 20 AM Blank 10N M 10 3 1 00E 00 D 515 5 Blank Default 11 13 2006 8 22 AM Blank 10 NaN nM 10 3 1 00E 400 1 74 nm1 D 515 B Standard Default 11 13 2006 8 26 AM Measure 10 0
46. User Preference Save Preference fi C AND 3300 DataWMefaulth4 Methyl Umbelliferone File Edit View Favorites Tools Help Qs Search ie Folders X 1 EB Address ED C ND 3300 DatalDefault4 Methyl Umbelliferone Folders x Mame Size Type Date Modified 4 Methyl Umbelliferone 2006 09 21 wv2 6 nfd 16KB NFD File 9 21 2006 2 52 PM 4 Methyl Umbelliferone 2006 10 03 v2 5 nfd 2KB NFDFile 10 3 2006 9 15 AM 4 Methyl Umbelliferone 2006 10 20 v2 6 nfd 19KB NFD File 10 20 2006 2 57 PM 4 Methyl Umbelliferone 2006 10 25 v2 6 nfd 4KB NFD File 10 25 2006 3 05 PM 4 Methyl Umbelliferone 2006 10 31 v2 5 nfd 2KB NFD File 10 31 2006 4 25 PM Data Viewer Data Viewer Data Viewer
47. A new copy of the password log file should appear in the c NND 3300 Data Log Foles folder Insufficient Memory 40 MB No Printer Connected PC
48. e Configuration Auto Scale Y Include graph in printout Include standards in printout e Data import data Ctrl I rename samples CtrlrN delete sample data Ctrl D Data Viewer e Report Reports Page e File Page Set up e Print Window Print Window CTL P Plot Report Standards e Save Window jpg e Help Context Help Context Help
49. LED Fluorescence Profiler The Wizard has derived the relative fluorescence units for each LED The RFUs are displayed to the right The peak RFU Spectrum fluorescence wavelengths for each LED are as follows white UV Blue Peak wavelengths Peak RFUs White 517 White 101 32 ov nn WAS Sue si Bue Ca Peaks Possible Sources 9 Blue nm Click Nextto acceptthe Indicated Source amp wavelengths 1 1 i 1 and to assign a name to the new method 500 550 600 Wavelength nm The threshold for peak detection is 30 0 RFU LED RFU LED Possible Source LED 6 1 SECTION 6 FLUORESCENCE PROFILER blank Back
50. 1W e CE UL CSA e Windows 2000 XP Function Key Function Keys Function Keys Measure F1 Save as Ctrl A User s Manual Ctr M Delete sample Rename Re blank F2 Data Ctrl D Samples Ctrl N Blank F3 Configure Report Ctrl F Print Window Ctrl P Print Load Report Sereen F4 Fofrrial Ctr G Print Report Ctrl R Print Report F5 Help Menu Ctrl H Exit Ctrl Q Recordind F6 Import Ctr Save Report Ctrl S Show Load Report Report F7 Format Ctrl L Sort Report Ctrl T NanoDrop 3300 methanol ethanol n propanol isopropanol butanol acetone ether chloroform carbon tetrachloride DMSO DMF Acetonitrile THF toluene hexane benzene sodium hydroxide sodium hypochlorite bleach dilute HCI dilute HNO3 dilute acetic acid 7 HF 130 0021 3 9 2
51. Defaull es System idle timeout 4 Default 30 Cancel 4 Default 3 3 SECTION 3 amp fE S fig e e 99 e Administrator Administrator
52. e Linear e 2ndor3rd order Polynomials Standard Curve Default Unit 6 Add New Analysis type 1 point Formula 1 point fluorescence acquisition module 1 RFU Formula 2 4 LED Edit method parameters Fluorescence Analysis Source Correction General For White LED illumination the virtual emission filter interval AA is symmetrically applied around each Analysis nm emission wavelength selected All RFUs outside of the virtual filter interval are setto zero The Default interval is 20 nm s
53. e Plots Sets 1 20 20 1 s Legend Legend Legend s Sample information Sample Information Data Viewer s x y x y
54. e Sort Report Ctrl T e Save Report Format all files nfd User Preference Change Setting e Load Report Format Ctr G e Print Report Ctrl R Report Configure Standards Plot e Save Report Ctrlr S Load Report CtrlrL
55. 150 pg Linear Range 75 ng ml 1500 ng ml Hoechst dsDNA 33758 File EdW Con amp gurason Standard Curves Help Rie blenk Frint Screen Recording View Standard Curve ser Detault 1 Windo Ext Blank Print Repon Show Raport 45 2008 3 04 PM Measure Hoechst dsDNA 33258 Source Load 1 to 2 ul of your sample and click the measure button RFU 450 341 8 RFU 450nm 341 8 270F0013 dsDNA PicoGreen dye ds DNA PicoGreen8 PicoGreen dsDNA LED 525 nm 20 nm 2ul PicoGreen6 2 pg Linear Range 1 ng ml 1000 ngm Eile EdW ConWigureWan Standard Curves Help Re blank Print Screen Recording View Standard Curve ser Default Repo Measure es in Blenk PrintRepot ShowRepor
56. C WVINNTNNF Windows XP C WWINDOWSNINF Driver X Configuration Failed You Must Manually Edit the Registry Windows2000 XP Error 8 Occurred at Open File Error 9000 password log 2
57. Tel 03 5625 9711 Fax 03 3634 6333 7532 0003 5 1 3 403 Tel 06 6394 1300 Fax 06 6394 8851 E mail webmaster scrum net co jp Internet www scrum net co jp ND141128A
58. 10 Account Management Modify User Administrator Change password Default Administrator Account Management Options Passwords log 7 C NNanoDrop Data Log Files ID Administrator
59. Auto Gain Fixed Gain Fixed Gain 0 10 Gain Next 4 1 SECTION 4 CREATE EDIT METHOD Edit method parameters Up to 4 wavelengths can be defined for logging and analysis as part of a method A1 is used for quantification if Analysis Type is 1 pt If Analysis Type is Formula quantification is based on a user entered equation using the RFU at wavelengths A1 through A4 of wavelengths xi 11 S NaN nm Standard Curve Type j Linear Analysis type f1 pt Default Units ng ml Quantification will be performed at A1 Standard Curve Type e interpolation linear interpolation AH
60. Spectrum SECTION 11 RIFT 4 DATA VIEWER s Import and Return Plots Reports PC shift control Plots Plots 3 830 L I 1 500 520 540 560 5 Wavelength nm e Method e Date e Selected Plot 2 Selected Plot Legend
61. Next 6 2 SECTION 7 JE E FI ZUEB OE 7 Open Protocol Load 1 In2ulgfynur sample and click the measure button e Blank F3 blank blank e Measure F1 Measure Measure Me
62. x nm Edit Selected Edit method parameters Fluorescence Analysis Source Correction General Select LED source UY Blue White m Excitation Spectrum AutoGain Dx Edit acduisittion View Selected 4 3 SECTION 5 USER PREFERENCES 5 User Preferences User Preferences 3 User Preferences User Defaut General Settings Archiving Reports Acquisit
63. 10 Exit 7 6 SECTION 8 NUCLEIC ACID QUANTIFICATION 8 Nucleic Acid Quantification 6 dsDNA 33258 Hoechst dye Quant iT DNA HS dsDNA PicoGreenG dye RNA RiboGreen dye Quant iT DNA BR Sybr amp Green Create Edit Method Create Method d Create Save Method dsDNA 33258 Hoechst ds DNA Hoechst Hoechst dsDNA UV LED 450 nm 20 nm 2ul Hoechst
64. 527 20 nm 2ul Sybr Green 2 pg T Linear Range 1 ng ml 1000 ng ml Eie Edt Con amp guraeson Standard Curves Help Re blank Print Screen Recording View Standard Curve Blenk PrintRepot ShowRepor Detault r E 4 15 2008 4 21 PM Measure ReserWindaw Measurement Type Method Sybr Green Source Blue Open Protocol Sample Standards Load 1 to 2 ul of your sample and click the measure button Sample ID SybrGreen 1 dsDNAl 120 04 Sample 100 0 80 0 2 60 0 40 0 Actual Gain 20 0 10 00 0 0 S00 525 550 575 800 625 650 675 Wavelength nm 270F0013 8 3 SECTION 9 PROTEIN QUANTIFICATION 9 Protein Quantification 4 Fluorescamine amp FluoroProfile amp Fluoradehyde OPA Quant iT Protein Create Edit Method Create Method
65. E 2i MIDO uoD0P 33 NanoD E Thermo Fisher Scientific
66. 658 nm 20nm DyLight 649 DyLight 649 674 nm 30 nm Alexa Fluor 649 Cy5 DyLight 680 DyLight 680 715 nm 30 nm Alexa Fluor 680 e Cy3 Alexa Fluor 555 Cy3 Alexa Fluor 555 565 nm 20 nm UV LED Alexa 555 10 2 SECTION 10 Z ftt FLUOROPHORES 10 3 SECTION 11 RIFT 3 DATA VIEWER 11 Data Viewer Data Viewer
67. Load 2 ul of blank solution and click the blank button to acquire blank spectra with each of the ND 3300 LEDs 500 550 600 Wavelength nm The threshold for peak detection is 30 0 RFU blank blank Measure 1 2 uL Measure 3 LED 30 RFU Fluorescence Profiler RFU Spectrum Load 2 ul of your sample and click the measure button white N to acquire spectra with each of the ND 3300 LEDs and PH UY to initiate fluorescence analysis Blue N Measure 500 550 600 Wavelength nm The threshold for peak detection is 30 0 RFU RFU 30 RFU
68. 4 15 2008 4 17 PM Ext j Load 1 to 2 ul of your sample and click the measure button 270F0013 8 1 SECTION 8 NUCLEIC ACID QUANTIFICATION RNA RiboGreen dye RNA RiboGreen RiboGreen RNA LED 525 nm 20 nm 2ul RiboGreen 10 pg 5 ng ml 1000 ng ml 2 dye File EdW Con amp guraeson Standard Curves Help Re blank Print Screen Recording View Standard Curve User Default Measure Reset Window Ext Blank Prnt Report Show Report 4 15 2008 4 10 PM z pma Measurement Type Method RiboGrwan RNA Source Blue Open Protocol il Sample Standards Load 1 to 2 ul of your sample and click the measure button Semples lt Actual Gain 10 00 i 1 i i i I 560 580 600 620 640 660 680 700 Wavelength nm 270F0013 Quant iT DNA BR Quan
69. 1i Data Viewer eeeesseseesesee rennen rennen nnnna nan 11 1 IRAT MIDI d 11 1 DAIVINGA E NS a 11 1 12 Source Check oo tr Eos a eos e enda re dl d e d 12 1 MM ird lS Ie cm 13 1 TIRAIRE NAJE A 13 1 Connection ENO Srat S a aS a cera 13 2 abra d hdd oM ds aa AE A A A AE AE EA 13 4 14 sg E54 MEE S cuuetontetncduiau t ated rei FJEDRE REDE enc Ene Ricco ED HT i HR SECTION 1 ZFZ amp 1 Thermo Scientific NanoDrop W 3300 LED 1 2 uL 3 LED 90 400 750 nm 2048 CCD 395 nm
70. 647 4 methyl umbelliferone Quinine Sulfate DyLight 405 DyLight 549 DyLight 633 DyLight 680 Create Edit Method Main Menu Create Edit Method button dye dye Create Edit Method Fluorescence Profiler Fluorescence Profiler LED Fluorescence Profile Data Viewer Data Viewer Data Viewer User s Manual PDF Users Manual Ctrl M
71. click Share this ffi CREATOR OWNER n folde Power Users ADMINANPower Users shang end securty To share thie folder wih bor network users and other users of this computer select the fiat check box below Permissions for Users Allow Deny Full Control Modify Read amp Execute v Share this folder on the network a List Folder Contents O Read Share name NancDiop 3 01 To configure settings for Offline access to this shared folder click Caching peche Wiite v v For special permissions or lor advanced settings m click Advanced Adyenced OK Cancel Appl C Ceres Lem E n 1 Windows has disables remote access to this computer Network Setup Wizard 2 log log read only log Incorrect command line parameters Unable to locate the LabView Run
72. 2 Std 3 Std 3 Std 4 Std 4 Std 5 Std5 ID conc RFU conc RFU conc E J conc conc E conc Microsoft Excel SECTION 12 SOURCE CHECK 12 Source Check Source Check LED LED Source Check LED Measure Source Check Source Check Technical Support LED Source Check Source Check File Show Context Help LED White Uv Spectrum Graph 3900 3500 3250 3000 2750 1 Ensure mea
73. Administrator password Log password Log 3 4 SECTION 4 CREATE EDIT METHOD 4 Create Edit Method Method FITC Bill PicoGreen Method Editor Method List 5 Method Editor Sort by Protected status Type amp Name xi Method List Method Type 34 Formula 13258 Nucl PicoGreen dsDNA Nucleic Acid Auto 525 Linear QuantiT DNA BR Nucleic Acid Auto 523 M 8 Linear Guantil DNA HS Nucleic Acid Auto 530 Linear RiboGreen RNA Nucleic Acid Auto i 525 Interp Sybr Green Nucleic Acid Auto 527 Linear Fluoraldehyde OPA Protein _ UN Auto li 455 Linear Fluorescamine Protein UN Auto 470 3 Linear FluoroProfile Protein Blue Auto 614 Y Linear GuantiT Protein Protein Blue Auto 600 3rd order 4 Methyl Umbelliferone Other UV Auto 70 450 Linear Cy3 Alexab55 Other White Auto 555 Line
74. Check File Show Context Help LED white Spectrum Graph 7000 6500 6000 Blue 5500 5000 1 Ensure measurement 4500 H 4 rii are clean and ry _ 4000 8 2 Select LED type above E 3500 White UV or Blue then e 1 L 3000 click Measure 2500 2000 1500 Cursor position nm 3 500 1000 500 Cursor value 1210 0 0 4000 450 0 500 0 550 0 6000 650 0 7000 7500 Wavelength nm PC C ND S300 DatalOperation amp Performance Images 12 1 SECTION 13 13 Error USB2000 Error USB2000 Unable To find Device with Serial USB 1 USB 2
75. Show Repon Page Setup Print indow Ctrl P save Window Exit Ctrl Q e Page Setup Report e Print Window Fie Ctrl P Print Print PC e Save Window File Save Window jpg Edit Edit SamplelD cut amp paste Eile Configuration Standard Curves Help Fim Repon ShowRepon Edith4ethoad lethod _PicnGreeh dsDNA Source Blue Configuration Configuration 5
76. nff Cy5 Alexab4 ND 3300 default report format nff DyLight 405 ND 3300 default report format nf DyLight 488 ND 3300 default report format nff DyLight 548 ND 3300 default repart format nff Reports Auto Report OChange Setting User Preference Save Preference 5 2 SECTION 6 FLUORESCENCE PROFILER 6 Fluorescence Profiler Fluorescence Profiler E FIX EEL IX2RATIO E EOMER 9362 7 v JUST BAO3Z amp LED Fluorescence Profiler Fluorescence Profiler RFU Spectrum
77. 00 Data Log files 13 2 SECTION 13 1 Administrator 2 C ND 3300 Data Log files 3 Sharing and Security Sharing 4 operating system networking XP Windows 2000 Professional XP Professional Home XP Professional Security Users Sharing share this folder Sharing Network sharing and Full Control security Al xl 3 General Sharing Security Customize Grou You can share this folder among other users on your roup or user names network To enable sharing for this folder
78. 00E 400 nM 10 3 1 00E 400 1 74 nm1 D 515 7 Standard Default 11 13 2006 8 31 AM Measure 10 5 00E 01 nM 10 3 1 00E 400 1 74 nm1 11 4 515 8 Standard Default 11 13 2006 8 33 AM Measure D 5 00E 01 nM 10 3 1 00E 400 1 74 nm1 11 5 515 9 Standard Default 11 43 2006 8 34 AM Measure 0 5 00E 01 nM 10 3 1 00E400 1 74 nm1 12 8 515 10 Standard Default 11 3 2006 8 41 AM Measure B 5 00E 400 nM 10 3 1 00E 00 1 74 nm1 188 4 515 11 Standard Default 11 13 2006 8 44 AM Measure B 5 00E 400 nM 10 3 1 00E 400 1 74 nml 186 8 515 12 Test samp Default 11 13 2006 8 46 AM Measure 5 4 1 10E400 nM 10 3 1 00E 400 1 74 nm1 38 6 515 13 Test samp Default 11 13 2006 8 48 AM Measure 10 8 45E 01 nM 10 3 1 00E 400 1 74 nm1 28 8 515 C W 3300 Data gt User name gt Application Module Hoechst dsDNA PicoGreen dsDNA RNA RiboGreen User Preference Archiving Duplicate Data Storage Duplicate Data Folder
79. 7 JE E FI JEBUD E Gain 1 7M BSEOBER UXeiXcd oleci hueJjd8ed4 cdd NanoDrop gain CCD e Auto Fixed Gain Auto Fixed Gain 2 Create Edit Auto Gain LED Actual Gain 10 0 1 1000 Fixed Gain Fixed Gain 0 1 0 1 10 0 RFU Auto Gain method edit
80. OPA Fluoraldehyde OPA assay NanoDrop3300 UV LED 455 20 nm 20 uL 2 uL 15 ug mL 15 ug mL 1000 ug mL 1 ug mL 1ug mL 50 ug mL Eile EdW Con amp gureson Standard Curves Help Re blank Print Screen Recording View Standar Reset Window ins Detout Ext Measure 4 16 2008 10 00 AM Blenk PrintRepot Show Report Measurement Type Method OPA Source UV Open Protocol Sample Standards Load 1
81. S LIH op dio qr d 2 1 mr ieri PI 1 2 RUN 2 1 JI ydg der LL Pm 2 1 E TIS INIT 3 1 FTIR RAAT E 3 1 FAIR VA T LOT LES RN E 3 1 FAASI E a E MIRROR RP 3 1 2 DE 00 3 2 tmm ettet bud e t eder 3 2 Account Mahagdement uidi tee e E E EA ER i 3 3 4 Create Edit Method nanan 4 1 Lp M ROE if e 4 1 S11 A A 4 3 SiUser Prelerences iiie ar 5 1 General Sellingds od eo i i 5 1 ATCRININGD al Se RR RE 5 2 REPONS cenos a E E mm 5 2 6 Fluorescence RIO 6 1 7 MAEMMMMMMMEEEMM nu m 7 1 MEES Ie 0D BEIDE esci etre E MEL REM AIRE e LES SORA OR SRI eR SEU 7 1 dae ETE RETE ET E OU 7 1 E E TP rd 7 2 8 Nucleic Acid Quantification eeeee ecce eeeeeee ee enne 8 1 dSDNA 353299 HOGSCHSLE sanc di e rrt RR a E Mena Eau LR d T as UMS 8 1 dsDNA PicoGreen dye eese nennen nennen nnne nns 8 1 RNA RiboGreetne dye rran tr rr RE Hn cron rr D Sada 8 2 Quanti T DNA B m Ni i i 8 2 Quanti T A B nd 8 3 SVOR GrG EM besneden T 8 3 9 Protein Quantificatlio ho SS 9 1 Flu rescamMiNE NR 9 1 FIuoroPsollle Ra i E sit oec i i i 9 1 Fluoraldehyde OPA 9 2 Quant4T Protein ASSaV iai seu a a n e pu RR 9 2 10 Fluorophore eene nnne 10 1 ED EU PE NETTEN 10 1 UV EEDA LU E NN 10 2 mE NIRE LUE qu TU S NITE E NP 10 2
82. ar Oy5 Alexab4 Other White Auto 665 Linear DyLight 405 Other UV Auto 40i 418 I Linear DyLight 488 Other Blue Auto 520 Linear DyLight 549 Other White Auto 558 em Linear v e 999992922925902929 29 E3 Note predefined methods are indicated with a diamond and cannot be modified Create Delete Edit View Method Selected Selected Selected Create Method LED Edit method parameters Select LED source UV Blue White Excitation Spectrum AutoGain pq e UV LED 365 nm e Blue LED 470 nm e White LED 460 650 nm Auto Gain Auto Gain Auto Gain
83. asure non blanks F1 Measure e Re blank F2 Re blank F2 Blank F3 Re blank e Print Screen F4 Print Screen PC e Print Report F5 Print Report F5 Data Viewer T C5 ND 3300 Data User nam
84. e Hoechst ds DNA PicoGreen dsDNA RiboGreen RNA e Recording F6 Recording Report Data Viewer Recording Start Report e Show Report F7 Show Report 11 Data Viewer ID e View Standard Curve Standard Curve RFU 1 7 1 5 e Open Protocol Open Protocol
85. e User s Manual for more guidance Disregard this measurement Accept this measurement Accept all low blank gains during this software session 1 Configuration Advanced Parameters User Preference Min Blank Gain Advanced Parameters User Preference 2 blank gain gain 1 Gain Min Blank Gain Technical Support amp High Gain Ratio Notification gain blank gain
86. erone 7 hydroxy 4 methylcoumarin 4 MU 4 methylum belliferone pH8 UV LED 4 MU Print Screen Recording View Standard Curve User Default WB re Arie B YET Show Repo Ext rint Report 4 4 2008 3 06 PM 4 Metfy Umbelliterone Load 1 to 2 ul of your sample end click the measure button 21550 0 i i i i i 1 i i i i i 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 Wavelength nm LED e Cy5 Alexa Fluo 647 Cy5 Alexa Fluor 647 670nm 30 nm 7 28 e DyLight 549 DyLight 549 568 nm 20 nm Alexa Fluor 555 Cy3 e DyLight 633 DyLight 633 Alexa Fluor 633 DyLight 633
87. ettable in the table below Note it can also be modified within the respective fluorescence method module Emission Windows Analysis Emission filter interval A23 565 20 A virtual emission filter interval AX nm virtual emission filter interval 0 20 nm 20 nm 4 2 SECTION 4 CREATE EDIT METHOD Edit method parameters Method name Type j Other Graph Min nm 480 Graph Max nm 700 2 e Graph Min nm x nm e Graph Max nm
88. ion Setup Confirmations Show Raw Spectra OFF Standards Units v ON Gain Notifications v ON Show Advanced Parameters OFF Silent Operation v ON Autoscale Plats v ON Gain Notification Settings Min Blank Gains 5 00 White 1000 5 UV 500 Blue Prompt User to Close Data Viewer OFF Max Gain Ratin General Settings General Settings e Acquisition Set up o saturation nm minimum blank gain maximum gain ratio advanced parameter y Data Viewer O Q e Confirmations o o Gain Notification Setting minimum blank gain maximum gain ratio e Gai
89. it Protein Source Blue Open Protocol vp Sample Standards Load 1 to 2 ul of your sample and click the measure button Sample ID Quant4T Frotein BSA 157870 0 140000 0 120000 0 5 3 Samplef lt 100000 0 80000 0 2 50000 0 40000 0 Actual Gain 20000 04 012 i i RFU 600nm 1332630 580 600 620 Wavelength nm 270F0013 9 2 SECTION 10 FLUOROPHORES 10 Fluorophore 11 e FITC FAM e Cy3 Alexa Fluor 555 Cy5 Alexa Fluor amp 647 Quinine Sulfate 4 methyl umbelliferone DyLight 405 DyLight 488 DyLight 549 DyLight 633 DyLight 649 DyLight 680 Create Edit Method Create Method Create Save Method LED e DyLight 488 DyLight 488 518 nm 20 nm Alexa Fluor 488 Cy2 e Fluorescein FITC FAM FITC 515 nm 20 nm
90. n Notification Settings gain CCD 10 0 gain 1 1000 Advanced Parameter gain e Min Blank Gain LED reference blank Low Blank Gain gain LED 5 UV LED 10 blank gain LED Min Blank Gain
91. name gt JI E E F Hoechst dsDNA PicoGreen dsDNA RiboGreen RNA Archiving Duplicate Data Storage Duplicate Data Folder User Preference Save Preference User Preferences User Defaut CSevePreeenes Ea General Settings Archiving Reports Custom Reports Hoechst dsDNA 33258 TRUE ND 3300 default repart format nff PicoGreen dsDNA ND 3300 default report format nff QuantiT DNA BR ND 3300 default report format nf QuantiT DNA HS ND 3300 default report format nff Repor RiboGreen RNA ND 3300 default report fnrmat nf TUTTO Sybr Green ND 3300 default report format nff Fluoraldehyde OPA ND 3300 default repart format nff Fluorescamine ND 3300 default repart fnrmat nf FluoroProfile ND 3300 default report format nf QuantiT Protein ND 3300 default report format nff 4 Methyl Umbelliferone ND 3300 default report format nff Oy3 Algxa555 ND 3300 default report format
92. onversion Archive File Converter Yi Selected Samples Sample ID Ose Time 4 gt gt gt Select gt gt gt lt lt lt Deselect lt lt d v Hold down Control or Shift keys to select multiple samples Import amp Return Cancel e Import Folder e Directory Tree e gt gt gt Or lt lt lt Selected Samples IZ L lt I Selected Samples e Search ID e Sample Information
93. op 1000 Thermo Scientific NanoDrop 3300 6 628 382 6 809 826 1 2 SECTION 2 2 IBM PC Mac e Microsoft Windows XP 32 bit 2000 e Windows Vista ND 3300 Windows 95 98 ME XP 64 bit Windows NT 233 MHz CD ROM 32 MB RAM 40 MB USB ND 3300 USB Microsoft Excel USB ND 3300
94. or RFU at XXX nm 20 nm 20 nm A virtual emission filter interval AX nm virtual emission filter interval 0 20 nm Method Editor 3
95. r floppy disk i O Yes this time only What do you want the wizard to do are automatically Her err er specific location amp dvanced Click Next to continue Click Next to continue Windows XP SP2 Windows OS 4 5 5 USBView Star Programs NanoDrop Utilities USBView USB USBView Downloads 6 Device Connected USB idVender idProduct 0x2457 0x 1002 USB
96. surement 2500 2o em are clean and 2250 8 _ 2 Select LED type above LSU White UV or Blue then o 1750 click Measure 1500 1250 1000 750 Cursor position nm 3 500 500 250 E Cursor value 106 0 i 4000 4500 5000 5500 6000 7000 750 0 Wavelength nm LED Source Check Source Check File Show Context Help Serial 0238 Spectrum Graph 4100 LED s 3750 UV 3500 Blue 3250 3000 1 Ensure measurement 2750 pedestals are clean and 2500 dry E 2250 E 2 Select LED type above E 2000 White UV nr Blue then 1750 click Measure 1500 1250 1000 750 500 250 Cursor position nm 3 500 Cursor value 524 6 0 4000 4500 500 0 5500 6000 650 0 7000 7500 Wavelength nm UV Measure UV UV LED UV LED Source Check Source
97. t iTW dsDNA Broad Range Assay dsDNA NanoDrop3300 dsDNA QuantiT dsDNA BR LED TH 523 20 nm 2ul Quant iT dsDNA Broad Assay 20 pg Linear Range 10 ng ml 5000 ng ml Quant iT DNA BR File EdW Con amp gureson Standard Curves Help Re blenk Print Screen Recording View Standard Curve Measure Blank Print Repon Show Report Measurement Type Method DuentiT DNA BR Sourcw X Blue Open Protocol 5 i NR ample andafds Reset Window User Default Exit 4 15 2008 4 28 PM Load 1 to 2 ul of your sample and click the measure button Sample ID 310 0 7 275 0 250 0 225 0 1 Sample 200 0 175 0 150 0 125 0 100 0 75 0 50 0 25 0 0 0 Actual Gain 190 00 40 0 i i i i i 500 525 550 575 600 625 650 675 Wavelength nm 270F0013 8 2 SECTION 8 NUCLEIC ACID QUANTIFICATION Quant iT DNA HS Quant iT dsDNA High Sensitivity Assay dsDNA NanoDrop3300
98. this software session Technical Support Technical Support Source Check LED UV UV LED s Save Manu jjpg Technical Support
99. time Engine C Program FilesNNanoDrop Utilities Run Time Installers LVRUNTIMEENG msi An Error Occurred Code 7 Source Open file in 3 dye list log passwords log user preferences log c NNanoDrop Data Log files NanoDrop Can t find file OOIDRV INI Error 7 Occurred at New File C NND 3300 Data C
100. to 2 ul of your sample and click the measure button Sample ID 21400 2000 0 1600 0 1600 0 1400 0 1200 0 gt 10000 800 0 600 0 A PFU 400 0 f Acuel Gain 455 18634 10 00 200 0 i i i i i RFU 455nm 16634 550 575 600 625 650 675 7 Wavelength nm i ugimi 27 0F0013 Quant iTW Protein Assay Quant iT protein assay NanoDrop3300 LED 600 20 nm 5ug mL 500 ug mL 2 dye 2 uL Quant iTprotein assay 10 ng Quant it Protein File EdW Con amp gureson Standard Curves Help Re blank Print Screen Recording View Standar Liser Detault Measure Reset Window Blenk PrintRepot ShowRepor es 4 18 2008 2 59 PM eH Measurement Type Method Quant
101. uoroProfile amp protein dye NanoDrop3300 FluoroProfile eppicocconone eppicocconone LED 470 nm 614 20 nm 3 ug Linear Range 3 ug ml 100 ugm 3 Eile Ed Conf amp guraeson Standard Curves Help Fe blank Frint Screen Recording View Standard Curve ser Default Measur Reset Windo Blenk PrintRepot Show Report 4 16 2008 10 55 AM Ext j Greece vae T FluoroProfile Source Blue Open Protocol VIEN TIU Sample Standards Min Blank Gaim 500 2 Max Gain io Load 1 to 2 ul of your sample and click the measure button I RFU 614 nm 379 2 amp 00 625 650 Wavelength nm ua mil 270F0013 9 1 SECTION 9 PROTEIN QUANTIFICATION Fluoraldehyde
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