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pELuc-test

1. U 1 0000000000 00 000 UDUDUUUUDDUUUDDDUD Dir TRWRIRIRIRIR AA A 00000000000000000000000000000000000000000 D luciferin OOODOOOOOOAOAOODOAOAODOOODOOAODAAAAODODDAAOOOOOOUAOAOAI OOOCOOOUUODOUDU 2 Emerald Luc Short life OO Code No ELV 201 000000000000000000000000000000000000 Z0000000000 O0 PESTO 0 00 0 OO Emerald Luc Short life OOO Code No ELV 201 7 OD S D O D D BOD OO 0000000000 Emerald Luc Code No ELV ONOOO 000000 3 0000000000000000000000000000000000000U000000000U000N HISOOOOOOOOOO U Emerald uc QO00000000 6Wvto010000000000000 2 0000 On DT NON OOO OOO II pe 5 MM y y y o f Cemo pELuc test Emerald Luc S hort life 0000000000 l pELuc PEST test ELV 201 3 Emerald Luc 10000 0 1 0000000000 Emerald Luc 100000 0000000 Pyrearinus termitilluminans OO 0000000000000 OODODODUUOODOOOOOODODODUUODOODODDODOUOOODOUODAOODODOUOOOIODUO 0000000000 D luciferin OO OOOOOOOOUUOOOUUOUOOUUDOOOOUOOIdu LLI 234107 0000000000000000000000000000000000000000N 510 H COOH OPE ATP O Rd Oxyluciferin AMP PPi CO D luciferin Mg O 2 Emerald Luc0000000 2 0E 04 1 5E 04 gt
2. SV40 Zz 1 0E 04 HSVik 5 0E 03 0 0E 00 fLuc ELuc fLuc 03 000000 0400000000000 Emerald Luc ELuoODOOOOOOOOOO fLuc Q Sv40 DO0000 00 Hsvtk 000000 0 Emerald NHH3T3 0 00000000000000000000000 Luc ELuc QO OO O000000 fluc U U OHeLa 00000000 0 2 mM D lucifernOg 00000000 s3 0 000000000000 00000240000000 AANA 0 2 mM D luciferin OO O0 OOD OOOO 00000 2 5E 06 ELuc 0 000 2 0E 06 05 000000000000000 a SV40000000 0000 Emerald Luc ELuc 00 0 00000 MA 000 fLuyy00 0000400 0 0000000000000 0000 50 05 000000000000Ewe00000000000000 00 00 0000000f4we00000000000000000000 120 000 A000 80000000000000001 000000 100 000000000000000000000000000000 80 00000000000000MO000000D000 100m 60 00000 ln 20 0 Emerald Luc 0000000 s38nmQ 0 OD O D 000000000 00000000 PHD I i IU D D DO OAAAOOADOADAOADADOAADADAODMTE Cco000000000000000 00000000000000000000000000000U0000 2 Short liife 00000000000000 0000000000000000000000000000000000000000000000000U10 000000000000000000000000000000000000000000000004 000 000 ornithine dehvdrogenasell 0 PESTO 000000000000000000000000000000 000000000000 0000 EOShert life 1100000000000000000000000000 0000000 000Emeradlwc C000 PEST00000000000000000000000000 1200000000U000U0N 6 OAAOAOIAADODOOOODOOAAAOAODOAODODOODO 02000000000 AO ELuc 0 6 000000000 Emerald Luc Short life 0000 ELuc Emerald Luc PEST PEST ELuc PE
3. PCROOOOI 0000000 000000000000000 00MutltiReporter Assay System Tripluc 0 GOOOOO000000J00000 pSLG pSLO pSLR 0 O0 OSLGOR F primer Code No MRV 401ODO 000000000 2000000000 00000000000000 Emerald Luc DD D DU UDD Ui U D D 000000000000 0000 0000000000000000000000 UDErerld Lc0000000000000000000 000 200000000000 O DOOOOMO000dinwe Gf OO 000000 029 1nMD luciferin Code No MRL 101 10200 0 O o0 O O O D O B O O OO OD au OU DOOAA IIu D D D D i D DD D Uu Uu iu HO u HOND D I I DO OOO O D D DD O dOOOOAODDOAODOAOODOUAAAODOI UDU OOADAODADAAOOAODOOODODAOOO dOODOODOAOODO 2 000Ginvtro0 00000000000000000 0000000000000000000000000mM0000000000000000000000 00000000000000000000000000000000 7 P10000000000000 1 pELuc test pELuc test Multiple cloning region 1 89 Emerald Luc gene ELuc 91 1719 SV40 late poly A signal 1733 1958 B lactamase Amp r gene 2052 2912 background reduction signal 3743 4222 2 pELuc PEST test pELuc PEST test Kpnl ZI 3743 Poly A ori Sspl 2032 Kpni ori amp Poly A Poly A pELuc 4222 bp cI Poly A AN test ELuc Emerald Luc Xba 11720 Not 11726 Sac 11959 BamH1 1977 Sacll 1965 m Smal 1971 Multiple cloning region 1 70 3873 Emerald Luc gene ELUc 91 1716 PEST 1717 1839 pELuc PEST test Econ 170 SV40 late poly A si
4. tgtggagctg agggacgt gg ccgtcgt ggg tatccctgac atcgaggccg gcgagct gcc ccaactcacc gctaaggagg tgtacgactt cctggcccag agggt gt ctc tcgtggactc tatccccagg aacgt gacag gcaagattag t cgaaaagag aagcttagcc atggcttccc gccggaggtg gaggagcagg ct gct ggcac gat ggaccgt caccctgcag cctgtgcttc tgctaggatc aat gt gt aga gctatt gctt tatttgtaac cgcccagaca tgat aagata catt gat gag ttt ggacaaa cat t at aagc tcagggggag gt gt gggagg ttttttaaag cct caaat at caacatttcc agt aaaagat gttttcgccc gtatccgctc gt gt cgccct gct gaagat c cgaagaacgt at gagacaat tattcccttt agt t gggt gc ttt ccaat ga gccgggcaag agcaact cgg tcgccgcata tctt acggat t gacaacgat gaaccggagc actattaact cactt ct gcg at tgcagcac acgaaat aga tttagatt ga ccttaacgt g gcgcotaatc tttttccgaa tt caagaact gt gt ctt acc agcccagct t ggcat gacag cggaggaccg t gaat gaagc ggcgaact ac ct cggccctt tggggccaga cagat cgct g ttt aaaactt agttttcgtt tgct gctt gc ggt aact ggc ct gt agcacc gggt t ggact ggagcgaacg t aagagaat t aaggagct aa cat accaaac ttact ctagc ccggct ggct t ggt aagccc agat aggt gc catttttaat ccacaact ag t gcaat aaac caagt aaaac aaccct gat a ttt gcggcat acgagt gggt tgagcacttt cactattctc at gcagt gct ccgctttttt gacgagcgt g tt cccggcaa gotttattgc tcccgtatcg ct cact gat t tt aaaaggat aat gcagt ga aagt t aacaa ct ctacaaat aatgcttcaa tttgccttcc tacat cgaac taaagttctg agaatgactt gccat aacca gcacaacat g acaccacgat caattaat ag t gat aaat ct ta
5. 00000000000 1 81 161 241 321 401 481 561 641 721 801 881 961 1041 1121 1201 1281 1361 1441 1521 1601 1681 1761 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2721 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 ctcgaggt cg acggtatcga taagcttacg gcccaccacc atggagagag agaagaacgt agat gct gta caacgctctg cacaagcact taccaggagt tcttcgacac tact gt gaag gatct gtgca gagaacaaca agagatt ctt acgaggacta tatcccagac gagct gt gta ctgcctaagg ttttggaggt taaagacaga gggct gcgag agcctgcaca acttcat gtc ctgtggacca ggtagccgcc atcctgtgct atctgtgtga gactcacaca cgcat ctgac gccattcttc cacgccttcg gcttcagt at ttaaccagga ggtgttcctg aaggccatcc ctgtccaaga gccctctggt ggacaagtac ggaggt ggcc gagatcgccg t gaagaggct ccaacatcca tactctgcac aacgagttca gacaggaaca ccggcgaggc cct gggt cca tot gaacaac ccacaggcta ctaaggaggc aggacgagta tttctacatc gtggaccggt gaggagatcc tccttcagca cccaggcat c agccggcttc gtggtgaagc agcccggcgc actccaagta cctgaggggc ggcgt aaggt ctgagggagg ccctgatgga gaaggctt ct gct gcccat g tcttgtgccc aggagagcgg tgccattctt ct agagcggc aaaaaat gct ttatttgtga aattt gt gat caacaattgc attcatttta tgtttcaggt gt got at gga gctcccgcgg cccgggggat taatattgaa aaaggaagag tat gagt att tgtttttgct cacccagaaa cgct ggtgaa tggat ctcaa cagcggt aag atccttgaga ctatgtggcg cggtattatc ccgtattgac ggtt gagtac tcaccagtca cagaaaagca tgagt gataa cactgcggcc aacttacttc ggggat cat g taactcgcct tgatcgttgg gectgtagca atggcaacaa c
6. 06 10 IPCRI LE Sh Emerald Luc Vector Series pELuc test Code No ELV 101 pELuc PEST test Code No ELV 201 DOOD TOYOBO CO LTD Life Science Department OSAKA JAPAN A3629 Emerald Luc WOU 0000 0 1 0000000000 2 Short life OOO 0000000000 3 O00000 OOOUDOOUUOOOOUAODLUOO 1 OUULLILOLULL LULI LULOLLOU 2 00000000 OOODODDUUDODODOOLO OOOODOAOUODOUODOUUODOO OUOOOOOOUUO 000 OOUUUUUUUWOUUUU UD 0000000000000000 U dOOOODOUOOOODODOODOO UUUOUULUOUDOULOULULU 02000000000000U00000 OUUUUUUUU 00000000 20000000 U U U U U U U 00 UU OOOUOODOODODOAOODO UD LU UU O UI EI UI II H UDDD 000000000000000000000000000000000000000000 000000 02000000000000000000 0000 DD ODI D D D H U DO OD DO D O D O DO D D DUN dOOODOODOAAODOOAOOAOOAAAODODDOODOBAOAOAAODODOOOODODAOAO UU UU UOULLOULL Emerald Luc 107000000000000000000000000000000000000000 OO UOU00000 Emerald Luc 00000000000000000H 0000 U Emerald Luc 0 DDDD UU UD UU DDOD 0 D lucifein 107000000000000000000000000 0 00 OOOOOOODDOOOOADOOAAODOUODOOOAOI 0000000 Emerald Luc 0000000000000000000000000000000000 OOOOODOOODODOAAODOOOAODAOOODOODOAODAAODOOAADOOAOAAAAOOODO OO OOOAAAOAOIODAODAAOUODODUAADDODAAAOAOUDOOOODAAOO 02000000000000000U00 101 ELuc ELuc PEST 00000 lt 000 7 00000 0000 00000 Din vitro 0 0 00 Din vivo 0000
7. O 1 0000060000000M 000000 pELuc test pELuc PEST test 1000000000000000000000000000000000 0000000000000000000 001 high fidelity O000000 PCR UD KOD Plus Code No KOD 201 KOD Plus Ver 2 Code No KOD 211M OOO DO OD 0000000 000000000000060O0000000000PCRO000000000000000000000 02000000000000000U00000 0 9 pELuc testi 000000000000 000000000000000000 OOOOOodOOOOUOO 5 CAATGI ATCITATCAT GICTGGN GATTITIT CACTGCATTC TAGITGIGGI TTGICCAAAC TCATCAATGI ATCTTATCAT GICTGGATCA TAATCAGCCA TACCACATTT GIAGAGGITT TACTTGCTTT AAAAAAGCTC COACACCTCC COCTGMICCT GMACATAAA ATGVATGCAA TIGTGTGT TAXTTGITT ATTGOACCTT ATAATGGITA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCTA TTTTTTTCAC TGCATTCTAG TIGIGGITTG TCCAAACTCA TOMATGIATC TTATCATGIC CTOGAGST CACGGTATC Xhol Sall Clal Banlll GITAAGCTTA GXIGIGTAG CGCATGOACA TCTACTAGTC GGOOGGATAT CGMATTCCTG COATGAGG Hind III y Mlul Nhel Sphl Bglll Spel EcoR V EcoRI Pstl Nco _ ELuc AGAGMGIC GIGGIGTAGG CKO CAAGCACCCT CTGGGCAACT TCACC CKKG aara GET GTTOGT s OO0000 00000000000 0 1 pELuc PEST est 0 0 000000000000 0000000000000000 0000000 0000 00000 OOOOOOOdOOgOOOOOOdAdAAOAOOOOOOOOOODOOOIUOOOOOAOO 00000000O0O0000 UD 00 OOo D SV40 poly A signal M on m ll
8. OODOODOOOADODODJ 00 OO mo oO aan HO go oOo Doc 80 g OO Hoog 20 g Oo Hp U DOOOOOOODDOODOOOOLU dOOOOOAOUAAOOOOgg 00 00000000 UDDUDD dm Or E EI U U U IE 000 00000 000 U OOOOOLO CI Endotoxin 00 ss EIE U 810000 1 0000000000 UDUUDUUDDDDDUDDU 3 4 230 232 2006 2 Li X Zhao X Fang Y Jiang X Duong T Fan C Huang C C Kain S R J Biol Chem 273 34970 34975 1998 3 Andrew G B Joanna B Cameron S O and Peter N C Plasmid 44 173 182 2000 13 10000 000000 EE EEN NE Se O High Fidelity PCRO 0 U KOD Plus High Fidelity PCRO DO KOD Plus Ver 2 Taa0 0000000 Blend D OHot start 00 ad 000 000 Ligation high 00000000 Competent high DH5a PCROOOOOOO InsertCheck Ready InsertCheck Readv Blue lt 000000000 00000000000000 0 0 DOpSLG pSLO pSLR U OO SLGOR F primer l On in vivo D lucif System Tripluc OO pSLG test em Tripluc pSLO test em Tripluc pSLR test System Tripluc pSLG SV40 control tem Tripluc pSLO SV40 control tem Tripluc pSLR SV40 control tem Tripluc O0 pSLG HSVtk control MultiReporter Assay System Tripluc SLO SLR TA Cloning kit MultiReporte
9. ST 7 Emerald Luc Short Hfe OD O0 O D O 3 0E 05 2 5E 05 P U Emerald Luc Eluc Emerald Luc Short life 00 ELuc PEST 0 00000 I CHODI I I I I i I I 000000000000000000000000000 0000000000000000000000e000 0 ELuclA 0 OO D OLuc PEST 00 0 U 0 QEmerald Luc Short life 00 ELuc PESDOOOOOOOOOOO 0000000000 0 0E 090 5 0E 04 08 000000000000U00 pELuc tesi pELuc PEST test 000000000000 00000000000000 Bma10000000000 00000000000NIH3T3 O D D 0000000000 00 000000000000 100nM000000000000 0 2mM D luciferin DD D D D D OD D DOO D O D O DO O D 00000030000000000000 00 ELucOO U ELlve PEST 00 0000 o o m Q o pELuc RLU min E gt n LLI a o 5 LLI o 48 Time hr 3 1000000 Emerald Luc 70 0000000 00 0 MultiReporter Assay System Tripluc 0000000000N 00 0000000000000000000000 U OEmeradLwc0000000000000000000 000 000000000000 p 800 00000 SV40 late poly A signal 000000000000 RNA Polymerase IO 00000000000000 3 0 HD L 2000 250 bp 5 O2000000000OUODOONOOOOOOORNA0000000000000000 Background reduction signal 00000000000000 AATAAA I 2000 SV40 early poly A signal 10000 0000000 00000000000000000000000000000000000000000000U0U 0000 00000000 000 OODD I ND ANNO OO O O O OO No OO O OA TR TR NO DD DNNN LLII II IL TITITITITITITI O OO OO D OO O OD O N O TIT p 7 TT 00 ODI AI DOOUQUOQUUUUUUDU00O
10. aaaaaac ctcccacacc tccccctgaa cctgaaacat caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt ttgtccaaac tcatcaatgt atcttatcat aaaaaacctc ccacacctcc ccctgaacct aacgcggcct ttttacggtt ccataccaca tttgtagagg ttttacttgc gcattttttt cactgcattc tagtt gtggt taccacattt gtagaggttt ttgttgttgt taacttgttt tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tg tttgccttcc aagcat t ggt cctggccttt tacttgcttt attgcagctt 2 pELuc PEST test U 1 pELuc PEST 00000 1000 20000000000 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gt gt gggagg ttttttaaag caagtaaaac ctctacaaat gtggtatgga gctcccgcgg cccgggggat atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tat gagtatt tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaact cgg ggttgagtac tcaccagtca cagaaaagca tctt acggat gccat aacca tgagt gat aa cactgcggcc aacttacttc tgacaacgat gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc t gaat gaagc gcct gtagca at ggcaacaa cgttgcgcaa actattaact ttactctagc ttcccggcaa caattaatag actggatgga ggcggat aaa gttgcaggac cacttctgcg ctcggccctt ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga togtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaat aga cagat cgctg aact gtcaga ccaagtttac tcatatatac tttagattga tttaaaactt ctaggt gaag atcctttttg ataa
11. acgt ggtgtacggc cccgagccca agcaccctct gggcaacttc accgccggcg 161 agatgctgta caacgctctg cacaagcact cccacatccc ccaggccatc ctggacgt ga tgggcaacga gt ccctttcc 241 taccaggagt tcttcgacac tactgtgaag ctgggccaga gcctccagaa ctgtggctac aagat gaacg at gtcgtgtc 321 gatctgtgca gagaacaaca agagattctt catccccatc atctccgcct ggtacatcgg catggtggtg gcccctgtga 401 acgaggacta tatcccagac gagctgtgta aagt gaccgg catctccaag ccgatcctgg tcttcaccac taggaagat c 481 ctgcctaagg ttttggaggt taaagacaga accaactaca taaagaggat catcatactg gactctgaag agaacctgct 561 gggctgcgag agcctgcaca acttcatgtc caggtactcc gacaacaacc tccaaacatt caagcctctg cactacgacc 641 ctgtggacca ggtagccgcc atcctgtgct cctccggcac aaccggcctg cctaaaggcg tgatgcagac ccacaggaac 721 atctgtgtga gactcacaca cgcatctgac cccagagtgg gtacacaact catccccggc gtatccgtgc tggcctacct 801 gccattcttc cacgccttcg gcttcagtat caacctgggc tatttcatgg tgggcctgag agtggt gatg ctccgaaggt 881 ttaaccagga ggtgttcctg aaggccatcc aggactacga gotgaggagc gtgatcaacg ttccctccac aatcctgttc 961 ctgtccaaga gccctctggt ggacaagtac gacctatcca ccctggcgga gctgtgctgt ggagccgctc ctctggcgaa 1041 ggaggtggcc gagatcgccg tgaagaggct gaacctgcca gggatacggt gtggctacgg tctaacagag tctacctccg 1121 ccaacatcca tactctgcac aacgagttca agtccggctc cctgggcaag gtgacacctt acatggccgc caagatcatc 1201 gacaggaaca ccggcgaggc cctgggtcca aaccaggtgg gcgagctgtg catctgggga cctatggtaa caaaaggcta 1281 tgtgaacaac ccacaggcta ctaaggaggc catcgacgac gacggctggc tgcactctgg cgacttcggc tactacgacg 1361 aggacgagta tttctacatc gtggac
12. cggt acaaggagct gatcaaatac aagggctatc aggtcgcccc tgtggagctg 1441 gaggagatcc tccttcagca cccaggcatc agggacgt gg ccgtcgtggg tatccctgac atcgaggccg gcgagct gcc 1521 agccggcttc gtggtgaagc agcccggcgc ccaactcacc gctaaggagg tgtacgactt cctggcccag agggt gtctc 1601 actccaagta cctgaggggc ggcgtaaggt tcgtggactc tatccccagg aacgtgacag gcaagattag t cgaaaagag 1681 ctgagggagg ccctgatgga gaaggcttct aagctgtaat ctagagcggc cgcccagaca tgataagata cattgat gag 1761 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 9 1841 1921 2001 2081 2161 2241 2321 2401 2481 2561 2641 2721 2801 2881 2961 3041 3121 3201 3281 3361 3441 3521 3601 3681 3761 3841 3921 4001 4081 4161 tattcccttt tttgcggcat agttgggt gc acgagt gggt tttccaat ga tgagcacttt tcgccgcata cactattctc agaat gactt taagagaatt at gcagt gct aaggagctaa ccgctttttt cataccaaac gacgagcgtg acaccacgat ccggctggct ggtttattgc tgataaatct agataggtgc ctcactgatt catttttaat ttaaaaggat ccactgagcg tcagaccccg tagaaaagat aaacaaaaaa accaccgcta ccagcgotgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact gcctacatac ctcgctctgc taatcctgtt caagacgata gtt accggat acctacaccg aact gagata cct acagcgt tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc tgctggcctt ttgctcacat gttctttcct ttt
13. gnal 1863 2087 B lactamase Amp r gene 2182 3042 4352 bp EE ET background reduction signal 3873 4352 Amp PEST Poly A Sca 2466 Abal 1550 Ssp 2162 Sac 12089 BamH I 21 7 SacI 2095 0 pELuc PEST testO S O D DOO O OD D Hind l JPst IONcIOOOOOO ELucOOOOOOOOOOOOOO 0000000000000000U000000000000000000000000O0D0O0DONOOODOUDOO 1000000000000000000 1 pELuc test U 1 pELuc 00001000 20000000000 OO OO 0000 OO UU 0000 OO UU 0000 Acc 2 7 1110 Eco RV 1 64 Pvu 1 2467 Alw 441 2 2167 3413 Ehe 1 1546 Sac 1 1959 Ase 1 2663 Fsp 1 2614 Sac Il 1 1965 Bam HI 1 1977 Hae II 2 1546 3483 Sal 1 7 Ban Ill 1 16 Hin 1l 2 1546 2298 Scal 1 2356 Bcl 2 932 1400 Hind III 1 22 Smal 1 1971 Bd 1 2715 Kpn I 1 3743 Spe I 1 52 Bg II 1 46 Mlu 1 28 Sph 1 40 Bst XI 2 83 285 Nar 1 1546 Ssp 1 2032 Cfr 91 1 1971 Nco 1 89 Styl 1 89 Cla 1 16 Nhe 1 34 Xba I 1 1720 Eco 521 2 58 1727 Not 1 1726 Xho 1 1 Eco 811 2 484 1611 PpuM 1 1257 Xm 1 2235 Eco RI 1 70 Pst 1 76 U 2 pELuc tesf 1000000000000000 Aat Aat Il Afi Il Apa Bbr PI Bfr Bau 11 Bsi W Bss HII Bst Ell Csp45l Cspl Dra lll Eco 105 Eco4171ll Eco T22 Mrol Msc Nde Nru Nsp V Pac Pvu Il SanD Sfi Srf pELuc test 100000 M000000000000000000000 1 ctcgaggtcg acggtatcga taagcttacg cgtgctagcg catgcagatc tactagtcgg ccggatatcg aattcctgca 81 gcccaccacc atggagagag agaaga
14. gttatcta aagcat t ggt ct aggt gaag ccact gagcg tcagaccccg t agaaaagat aaacaaaaaa accaccgcta ccagcggt gg ttcagcagag cgcagatacc aaatactgtt gcctacatac ctcgctctgc taatcctgtt caagacgata gttaccggat aaggcgcagc acctacaccg aact gagata cct acagcgt gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga ctggtatctt gcct at ggaa 11 tatagt cct g aaacgccagc tcgggtttcg ccacctctga cttgagcgtc aacgcggcct ttttacggtt cctggccttt 3841 3921 4001 4081 4161 4241 4321 tgctggcctt ttgctcacat gttctttcct gcggtaccga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagtt gt ggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt at aat ggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tg 12 7 OOOAOOUOOOO 1 DOOOO0 00000000 PCRO0O00000 00000000 cc0o0000000000000 PcR0000000000000000007000000 PCR0000000000000000000000 0 PCR D 200000000 0000 PCR Denature 0000 98 q 100 0000 U S 0 0 DMSO PCROODOOUOOUOOO 000000000 PCR OAOOAA OAOODAADAAAOOODMOO 5000 OU 000000000 PCRO00000000000000 20000000 2 OOODOOADO OOOOOOOODOOOODUODDOD OAODOA
15. gttgcgcaa act ggat gga ggcggat aaa gttgcaggac ggagccggtg agcgtgggtc tcgcggt atc cacgacgggg agt caggcaa ctat ggat ga aact gtcaga ccaagtttac tcatat atac atcctttttg ataatctcat gaccaaaatc caaaggat ct tcttgagatc ctttttttct tttgtttgcc ggat caagag ctaccaact c cttctagt gt agccgtagtt aggccaccac accagt ggct gctgccagtg gcgat aagt c gotcgggctg aacggggggt tcgtgcacac gagctatgag aaagcgccac gcttcccgaa gcgcacgagg gagcttccag ggggaaacgc gatttttgtg atgctcgtca ggggggcgga cgtgctagcg cat gcagatc tactagtcgg ccggatatcg aattcctgca ggt gt acggc cccgagccca agcaccctct gggcaacttc accgccggcg cccacat ccc ccaggccatc ctggacgtga tgggcaacga gtccctttcc ctgggccaga gcctccagaa ctgtggctac aagat gaacg at gtcgt gt c catccccatc atctccgcct ggtacatcgg cat ggt ggtg gcccct gt ga aagt gaccgg catctccaag ccgat cct gg tcttcaccac taggaagat c accaactaca taaagaggat catcatactg gactctgaag agaacct gct caggtactcc gacaacaacc tccaaacatt caagcctctg cactacgacc cctccggcac aaccggcctg cctaaaggcg tgatgcagac ccacaggaac cccagagt gg gtacacaact catccccggc gtatccgtgc tggcct acct caacctgggc tatttcatgg tgggcct gag agt ggtgatg ct ccgaaggt aggact acga ggt gaggagc gt gat caacg ttccctccac aatcctgttc gacct atcca ccctggcgga gctgtgctgt ggagccgctc ctctggcgaa gaacct gcca gggatacggt gtggctacgg tctaacagag tctacctccg agt ccggctc cctgggcaag gtgacacctt acatggccgc caagat cat c aaccaggt gg gcgagct gtg catctgggga cctatggtaa caaaaggcta catcgacgac gacggct ggc tgcactctgg cgacttcggc tactacgacg acaaggagct gatcaaatac aagggctatc aggtcgcccc
16. r Assay System Tripluc SLO SLR TA Cloning kit for KOD MultiReporter Assay System Tripluc In vitro Detection Reagents Lysis Solution Assay Reagent 14 0 1mlx101 PIK 151 1000 D PIK 251 200 pmoles MRV 401 20 mg MRL 101 100 mg MRL 102 20u g MRV 101 204 g MRV 102 204 g MRV 103 204 g MRV 201 204 g MRV 202 204 g MRV 203 204 g MRV 301 10000 MRA 101 5000 0 MRA 102 IPCRI EE LU III UI UL 00000000 OOMDOOOAADOOAOIO OOOOOOOOUOO MUU U 530 8230 10000000000 20 80 TEL 06 6348 3786 FAX 06 6348 3833 E mail order lifescience bio toyobo co jp OOOOOOOOUOO OU 103 8530 00000000000 170 90 TEL 03 3660 4819 FAX 03 3660 4951 E mail order lifescience bio toyobo co jp OOOOOIDUDOODDOODODO OOOOuAOOu TEL 06 6348 3888 FAX 06 6348 3833 0000 9 000 12 00 13 000 17 00 1000000 00 E mail techosk bio toyobo co jp URL http www tovobo co jp bio
17. tctcat gaccaaaatc ccttaacgtg agttttcgtt caaaggat ct tcttgagatc ctttttttct cctcaaatat gtatccgctc caacatttcc gt gtcgccct ggcat gacag cggaggaccg ggcgaact ac gcgcgtaatc tgctgcttgc ct gtagcacc accagt ggct gctgccagtg gcgataagtc gtgtcttacc gggtt ggact aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg gagct at gag aaagcgccac gcttcccgaa gggagaaagg cggacaggt a tatagt cctg gcggt accga t cat aat cag aaaat gaat g cacaaat aaa gt ct ggatca taatcagcca gaaacat aaa at gaat gcaa at aat ggtta caaataaagc aatagcatca caaatttcac aaataaagca 00 00 0000 00 UU 0000 Acc 2 7 1110 Eco RV 1 64 Alw 441 2 2297 3543 Ehe 1 1546 Ase 1 2793 Fsp 1 2744 Bam HI 1 2107 Hae Il 2 1546 3613 Ban Ill 1 16 Hin 1I 2 1546 2428 Bcl 2 932 1400 Hind III 2 22 1711 Bg 1 2845 Kon 1 3873 Bg Il 1 46 Mlu 1 28 Bst XI 2 83 285 Nar 1 1546 Cfr 91 1 2101 Nco 2 89 1719 Cla 1 16 Nhe 1 34 Eco 521 2 58 1857 Not 1 1856 Eco 811 2 484 1611 PpuM 1 1257 Eco RI 1 70 Pst 2 76 1805 10 00 OO 0000 Pvu 1 2597 Sac 1 2089 Sac Il 1 2095 Sal 1 7 Scal 1 2486 Smal 1 2101 Spe 1 52 Sph 1 40 Ssp 1 2162 Sty 2 89 1719 Xba 1 1850 Xho 1 1 Xm 1 2365 U 2 pELuc PEST test 01000000000000000 Act Csp Pac Act Il Afl II Apa Dra Ill Eco 1051 Eco4all Pvu Il SanD Sfi Bbr PI Bfr Bsi W Bss HI Bst Ell Csp451 Eco T22I Mrol Msc Nde Nru Nsp V Srf pELuc PEST test 1710000 MO0000000 0

pELuc-test

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