One Step SYBR® High Speed RT
Contents
1. 1 2 1 180 60 2 0 1 DEPO ARC 37 C 12 DEPC 120 30 RNA RNA 180 60 DEPC 0 1 DEPC RNA RNA RT PCR RNA RNA RNA2. http wwwr takara bio coJp 10 RRO76A http wwwr takara bio coJp 11 RRO76A NOTICE TO PURCHASER LIMITED LICENSE P5 PCR Notice Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 994 056 and 6 171 785 The purchase of this product includes a limited non transferable im munity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commer cial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchas ing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA L11 SYBR Green I This product is covered by the claims of U S Patent No 5 436 134 and 5 658 751 and their foreign counterpart patent claims Takara PCR products containing SYBR Green are sold under license from Mol
3. total RNA NucleoSpin RNA II 740955 10 50 250 AGPC RNAiso Plus 9108 9109 http wwwr takara bio coJp 8 RRO76A B PCR 80 150 bp 300 bp 725 mer 40 60 45 55 Forward primer Reverse primer Tm Tm OLIGO 63 68 C Primer3 2 60 65 C GC AT 3 T C polypyrimidine A G polypurine 3 3 GC AT
4. 1 2 RT PCR http wwwr takara bio coJp 9 RR076A IX X One Step SYBR PrimeScript PLUS RT PCR Kit Perfect Real Time RR096A B One Step SYBR PrimeScript RT PCR Kit Perfect Real Time RRO66A B One Step SYBR PrimeScript RT PCR Kit II Perfect Real Time RRO86A B One Step PrimeScript RT PCR Kit Perfect Real Time RRO64A B SYBR Premix Ex Tag Tli RNaseH Plus RR820A B SYBR Premix Ex Taq Tli RNaseH Plus RR420A B SYBR Premix DimerEraser Perfect Real Time RR091A B Premix Ex Taq Probe qPCR RR390A B Thermal Cycler Dice Real Time System TP900 Smart Cycler Il System SC200N SC210N RNAiso Plus 9108 9109 NucleoSpin RNA II 740955 10 50 250 EASY Dilution for Real Time PCR 9160 Hyper PCR a Fujimoto T Konagaya M Enomot M Tsuboi K Hashimoto K Taniguchi K Kodama T Okabe N
5. 3 G C 3 T 3 base 3 2 base BLAST 3 Tm 1 OLIGO Primer Analysis Software Molecular Biology Insights 2 Primer3 http www genome wi mit edu ftp distribution software 3 http www ncbi nlm nih gov BLAST Perfect Real Time RT PCR amp PCR SYBR Premix Ex Taq M Tli RNaseH Plus RR820A B SYBR Premix Ex 7gg Tli RNaseH Plus RR420A B 2 RT PCR 1 RT PCR
6. Master Mix RNase Free dH20 Master Mix 2 PrimeScript II RT Enzyme Mix High Speed PCR Enzyme 500 20 20 3 5 x One Step High Speed Buffer Vortex 4 5 Random Primer
7. 3 PCR http www takara bio co jp 6 RRO76A VII Smart Cycler Il System 1 1 gt 5 X One Step High Speed Buffer 5 ul dNTP Mixture 10 mM each 0 5 ul Ywhaz F primer 20 u M 1 0 5 ul Ywhaz R primer 20 uM 0 5 ul PrimeScript Il RT Enzyme Mix 0 5 ul High Speed PCR Enzyme 1 5 ul SYBR Green 1 ul template 2 2 ul RNase Free dH20 13 5 ul total 25 ul 1 Perfect Real Time Primer 2 Mouse heart total RNA 1 pg ul 10 pg ul 100 pg ul 1 ng ul 10 ng L 2 Smart Cycler Smart Cycler Stage 1 Stage 2 Stage 3 Stagel Repeat 1 times Repeat 40 times Melt Curve v 45 C 14 2 Temperature Cycle V 3 TemperatureCycle v Start End Optics DegiSec 95 C 1 Deg Sec Temp Secs
8. 2010 Japanese journal of infectious diseases 63 31 35 b 2009 PCR 36 3 73 78 1 2005 3 4 PCR 120 126 2 2008 PCR 3 1 RT PCR 39 43 SYBR Molecular Probe Inc
9. http wWW taKara DiO CO jp SINT AG 2 v201201Da
10. RNA cDNA High Speed PCR Enzyme PCR 1 PCR SYBR Green 1 PCR PCR DNA DNA DNA 3 1 100 High Speed PCR Enzyme PCR 2 RT PCR RNA PCR RNA cDNA PCR RNA RT PCR RNA 1 RT PCR 1 1 RT PCR PCR Specific Primer Reverse cDNA Spe
11. LLLLLLLLLLLLLLLLT 3 ll 100 25 ul RIGA Ill oy pu a PrimeScript Il RT Enzyme Mix 50 ul High Speed PCR Enzyme 150 ul 5 X One Step High Speed Buffer 500 ul dNTP Mixture 10 mM each 50 ul 250 x SYBR Green 40 ul RNase Free dH20 1 mlX2 1 RNase Inhibitor 2 Mg 1 PCR authorized instruments 2 3 PCR 4 PCR VIII B 20 C 250 X SYBR Green 20 http www takara bio co jp 4 RR076A IV 1 Q 1 V 1 10
12. Oligo dT Primer http wwwr takara bio coJp 5 RRO76A VI RNA VIII A RNA 1 gt 5 X One Step High Speed Buffer 5 ul dNTP Mixture 10 mM each 0 5 ul Forward primer 20 u M 0 5 ul Reverse primer 20 uM 0 5 ul PrimeScript Il RT Enzyme Mix 0 5 ul High Speed PCR Enzyme 1 5 ul SYBR Green Tul template 2 1 pl RNase Free dH2O 14 5 ul total 25 ul 1 250 x SYBR Green RNase Free dH20 10 250 X SYBR Green 2 ul RNase Free dH20 18 pl 2 total RNA 20 pg 20 ng templete 2 PCR Stage1 45 C 1 95 1 Stage2 3 Step PCR Stage2 2 De PCR 98 C X 1 98 C 1 55 2 y gpl 40 50 60 C 2 oe Jas OR 68 Z 1 Stage3 1 PCR 2
13. Optics Degiec Temp Secs Optics 80 0 95 0 Ch1 0 2 Stage2 PCR NA 45 0 60 Off NA 98 0 1 Off NA 95 0 60 Of NA 550 1 Off Repeat 40 cycles NA 68 0 B On 98 C 1 55 C 1 68 6 _ Advance to Next Stage _ Advance to Next Stage Stage3 Melt Curve 8 2 Smart Smart Cycler Il System http www takara bio co jp 7 RR076A VIII Appendix A RNA RNA DS cDNA PCR cDNA RNA RNase RNase RNA RNase RNA
14. cific Primer Forward Reverse PCR Random Primer Oligo dT Primer http wwwr takara bio coJp 2 RRO76A Specific Primer Reverse Synthesis of 1st strand cDNA with PrimeScript Il RTase Synthesis of 2nd strand cDNA with High Speed PCR Enzyme Specific Primer Forward 1 One Step SYBR High Speed RT PCR Kit Hyper PCR GR http www takara bio co jp 3 RR076A 3 ASH DNA SYBR Green ZE DNA DNA 1 F F H F F F LLLLLI GL TILILLJTLlTLLLILS T 2 F F e F i cd F F L
15. ecular Probes Inc only for the usage in Real time PCR for internal research purpose These products are not to be used for the purpose such as providing medical diagnostic or any other testing analysis or screening services or providing clinical infor mation or clinical analysis in return for compensations L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries L46 SYBR Melting Curve Analysis The purchase of this product includes a limited non transferable license for all fields other than human or vet erinary in vitro diagnostics under specific claims of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technology Inc and Roche Diagnostics GmbH to use only the enclosed amount of product according to the specified protocols No right is conveyed expressly by implication or by estoppel to use any instrument or system under any claim of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 other than for the amount of product contained herein M57 LA Technology This product is covered by the claims 6 16 of U S Patent No 5 436 149 and its foreign counterpart patent claims Takaka 7 Tel 077 543 6116 Fax 077 543 1977
16. wea k RRO76A Talana One Step SYBR High Speed RT PCR Kit Hyper PCR v201201Da One Step SYBR High Speed RT PCR Kit Hyper PCR amp SYBR Green I 1 RT PCR v BCS 2 RT PCR 1 PCR PrimeScript ll RTase PCR RT PCR PCR 30 1 Molecular Probes Inc SYBR Green 1 SYBR Molecular Probes Inc 2 TaqMan 1 RT PCR 1 One Step SYBR High Speed RT PCR Kit Hyper PCR PrimeScript II RTase
Download Pdf Manuals
Related Search