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ALR-102

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1. Q UTP Q UTP RNA polymerase T7 RNA polymerase 881767 Roche T3 RNA polymerase 1031163 Roche SP6 RNA polymerase 810274 Roche 10 x Buffer RNA polymerase Buffer RNa
2. Q UTP DNA In vitro PE RNA Q UTP C s RNA UTP NTP ALR 102 RNA TGase RNA ALR 103 TGase TTP 5 ATP 20 mM 20 pL CTP 20 mM 20 pL GTP 20 mM 20 pL UTP 10 mM 40 pL Q UTP 10 mM 16 LL 1 RNA polymerase 2 UTP Q UTP 10 mM 20
3. Q UTP Q UTP 3 ALR 108 400 ng Q UTP in vitro Q UTP RNA PCR UTP Q UTP 1 kbp in vitro Mi 100 bp 1 UTP Q UTP 90 10 2 UTP Q UTP 80 20 3 UTP Q UTP 60 40 4 UTP Q UTP 40 60 1 4 T7 RNA polymerase 20101203 RN1 3762Rev0
4. RNA in witro in vitro G 50 RNA Roche 40C 2 ALR 108 Stop solution 4 LL TGase Hybridization Buffer 42 70C 30 Hybridization Buffer 9 10 ng mL 42 70C Wash 1 Buffer 2 42 70 10 2 1 Wash 2 Buffer 5 2 1 Hybridization Buffer 2 M Urea 20 mM phosphate buffer pH8 0 0 5 M NaCl 1 mg mL torula yeast RNA 1x Denhardt s solution 1 Casein 0 1 Triton X 100 5 Dextran sulfate MW 500 000 2 Wash 1 Buffer 50 mM phosphate buffer pH8 0 150 mM NaCl 0 1 Triton XX 100 3 Wash 2 Buffer 100 mM NaC1l 100 mM Tris HC1 pH9 5 50 mM MgCls 0 1 Trito
5. ALOKA Labelling ONE 20101203 RN1 3762Rev0 RNA UTP NTP ALR 102 2 Q RNA Q UTP UTP 857 RNA polymerase RNA Q UTP RNA ALR 103 20C 1 20C 6 20C
6. n X 100 181 8622 6 22 1 0422 45 5181 http www aloka co jp e mail bio aloka co p
7. se inhibitor RNaseOUT 10777 019 invitrogen Water PCR G 50 RNA 1274015 Roche mini Quick Spin RNA Columns G 50 1814427 Roche 1 in wtro PCR PCR 87 2 10 x Buffer ET DNA 200 ng 10ng uL RNase inhibitor 40 U L 1L 2U0 L ATP 20 mM 1 1mM CTP 20 mM 1L 1mM GTP 20 mM 1 1mM UTP 10 mM 12pL 06mM Q UTP 10 mM 08pL 04mM RNA polymerase 2p 40U Water to 20 pL UTP Q UTP UTP Q UTP 60 40 2

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