Cycleave®PCR Salmonella Detection Kit Ver. 2.0
Contents
1. S m lk Epoch Biosciences Inc US20020155484 WO0142505 WO02099141 US10 113 445 US09 876 830 Epoch Biosciences Inc Epoch Biosciences Inc GE A B2. DNA PCR DNA PCR DNA DNA dH20 SIN Positive Control invA ll 20 C I PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760
3. 20 V 2 1 invA 4 1 1 20 ul 1 WAK 5 2 1 2 x Cycleave Reaction Mixture 12 5 ul IX SIN Primer Probe Mix 5 X conc 5 ul x or SIN Positive Control or Su gt dH20 25 ul Total 25 ul
4. Omit Dle gle 2 TR PHT Pr J or KOODE E i i 7 g ARCA PDF EES Peer ee f hi a ROX 2 5 1 cat eget oe Er r F mari hy Fa H FI aa F z Fe ph elt Xa F n Ro 4 r E i 1 i mi i shy mm ba TT TT A rere tere FET TT A TI ETTWT a 1 T JI i Pri T 7 2 i Y Ac el A oT ir FT E Tt Wb w w af whe T mi 2 a i he pi ee PPE n PRT Ceen m ee re a eee I z i G Banan pam a z ik an F L 2 FAM IC ROX http www takara bio coJp 8 CY205
5. C 0 Tel 077 543 6116 Fax 077 543 1977 NANOGEN http www takara bio co jp http www takara bio co jp 15 CY205 NOTICE TO PURCHASER LIMITED LICENSE L4 Quencher This product is for measurement of amplification detection for research use only in life sciences re search industrial and environmental testing including food industry but excluding bio terrorism and bio warfare non human animal diagnostic testing forensic testing and providing services to third parties who do not use the services for the purpose of a providing patient management or care and in which the results of such services are not included in patient records and b providing bio terrorism or bio warfare testing and specifically excluding without limitation I any human clinical therapeutic or diagnostic uses and animal clinical and therapeutic uses including any use of the results of any testing performed with any product for patient management or care or the use of the results of the services for patient management or care and Il any
6. baa le nara Nis i oE z ai afl ET ones PPT fies F PFFT PFTT T p b 1 r LILI A EB Ee ee bs tt StepOnePlus Real Time PCR System Life Technologies ROX target ROX IC FAM ROX target http www takara bio co jp 12 CY205 Vi 1 ho pN invA invA invA er oT invA lt 3 2 SIN Positive Control mox pNW ar a FF invA invA I
7. mv4 FAM ROX 4 DNA PCR Ver 2 0 aos gt Lot AK701 EU Smart Cycler System Thermal Cycler Dice Real Time System Applied Biosystems 7500 Fast Real Time PCR System QO OQO Qr Oe DD Bl http www takara bio co jp 9 CY205 lL 25 ul Ris
8. Thermal Cycler Dice Real Time System Software Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 95 1 8 0 2 ml Thermal Cycler Dice Real Time System 0 2 ml 8 strip tube individual Flat caps NJ600 http www takara bio co jp 4 CY205 1 000 wl 200 ul 20 ul 10ul 4 IV 1 PCR 2 ae ee 3 Ph 4
9. Takaka Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp DIV 7A ARTS 41 v201402Da
10. 3 YY 1 2 3 5 PCR PCR Auto PC Manual V lees 2 PCR
11. ROX FAM ROX IC i jm p a toaa E anizi id n aa N a a M a aoaia ad alai g SEET Arran 3 gt http www takara bio co jp 9 CY205 6 gt c Cath hH o a 1 2 a ais 6 rel eho sl j mi a oa By Buu ra i k ae s wt ek 3 Ely B LA UI wy AI 4 gx Ku bit Ce ay Oy pi uy mls ete Ej UIE Sa ae et a a og F ie ae i ne PaPa y Te voa m 1 i F K nm Hl E Er im a I N gt P gt OK OUT gt Posi Nega N
12. Run Auto Auto Manual lt gt 5 2 1 2 NC PC NC N gt FAM ROX FAM ROX IC ta to a m 4 75 a a ee Ey Bi a h fla 4 t Perera ee eee OA m D i ee eee a zi i TP a4o08 34501 PC P gt FAM
13. 3 J J PCR i 4 J http www takara bio co jp 5 CY205 V 1 2 1 10 ul 1 5 ml 2 WAAK 90 ul 3 95 5 4 12 000 rpm 4 10 INVA 1 5 u X PCR AUS RIZE ZK CS 10 100 PCR
14. Thermal Cycler Dice Real Time System 1 CycleavePCR Kit gt TAR AlTE ABI RAD i eee X CycleavePCR Kit Thermal Cycler Dice Real Time System Software lt PM Plus Minus Assay gt Thermal Cycler Dice ital th System 2 PCR Hold Cycle 1 95 C 10 3 step PCR Cycle 45 95C 5 55 C 10 72 C 20 ami tsb ERY 3 BIA FO http www takara bio co jp 7 CY205 4
15. Primer Probe Mix DNA 3 ND gt http www takara bio co jp 10 CY205 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 1 Advanced Setup New Experiment 2 Experiment Properties Quantification Standard Curve TaqMan Re agents Other Other Include Melt Curve OY 3 Plate Setup Define Target Target Name SIN Reporter FAM Quencher none 4 Plate Setup Define Target Target Name IC Reporter ROX Quencher none 5 De
16. CY205 IdRaRa Cycleave PCR Salmonella Detection Kit Ver 2 0 v201402Da sss 3 BF a ea E E eee gs ecco pet EE E esse 4 oo 4 NN 4 OASE N NE E S 5 oo 5 ee OO 6 V 2 6 V 3 PCR 7 Thermal Cycler Dice Real Time System ui 7 Applied Biosystems 7500 Fast Real Time PCR System 11 Ye ci ne renee ee eee 13 VII PCR ee 14 oo 14 PR 14 EE 15 http www takara bio co jp 2 CY205 2 500 Salmonella Enteritidis F invA PCR Hot Start PCR
17. SIN Positive Control 2 3 2 3 SIN Positive Control PCR 1 http www takara bio co jp 6 CY205 V 3 PCR 3 PCR P C Z Cik Thermal Cycler Dice Real Time System 7500 Fast Real Time PCR System Life Technologies
18. lg Ex Tag HS sae RNA DNA RNase H RNase H RNA 1
19. x5 http www takara bio co jp 13 CY205 Vil PCR BUTE celta RAE EEM 225 ml 225 ml 35 C 18 24 37 C 18 RAE 225 ml 37 18 PCR PCR VII 1 Saiki R et al 1985 Science 37 170 172 2 1995 16 BAAR MME SS Shikai ee SE pp61 3 Galan J E et al 1992 J Bacteriol 174 4338 4349 4 Ashok K et al 1994 Microb Pathog 19 85 95 IX Viable Sa monella Selection Kit for PCR 7711 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 96 well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 Plate Sealing Pads 9090 0 2 ml Hi 8 Tube NJ300 0 2 ml Hi 8 Flat Cap NJ302 0 2 ml 8 strip tube individual Flat Caps NJ600 http www takara bio c
20. X 50 1 2 x Cycleave Reaction Mixture 2 X conc 625 ul 2 SIN Primer Probe Mix FAM ROX 5 X conc 250 ul C 3 dH20 1 ml 4 SIN Positive Control 150 ul 30 k 2 x Cycleave Reaction Mixture ESR Buffer dNTP mixture DNA PCR ECT SIN Primer Probe Mix 7 4 v4 DNA FAM invA ROX DNA v4 Dee Co DNA DNA
21. D PCR Eror X 1 OUT gt 2 DNA OUT PCR ROX FAM
22. NVA invA 4 3 3 ho pN Aty AP invA invA invA a nvA Meg a Se 3 x1 DNA 4 K2 3 PCR BR x4 AD SIN Positive Control
23. fine Samples NC PC 6 3 4 5 Plate Layout Passive Reference none 7 Instrument Peaction Volume Par mw 7 jil Fipan Wode Hold Haasse 8 cing hm Cycle 1 Tr 95 C 10 pD Slartny Cycle I 3 step PCR Cycle 45 8 tec TS 95 C 5 55 C 10 F 0 ee 72 C 25 gan a a 8 Start 9 Analysis B Amplification Plot Target Threshold Baseline Manual E 5 s SIN Target FAM http www takara bio co jp 11 CY205 a EN fein gee sess ee Seeds ter ae B EIEE lc 1 L if Enis p T rp i EE Laes nr 2 i i 5 mi p he ee a i i mies E ii i iai m a i i Bi Si ii a Internal Control ROX 10 View Well Table
24. o jp 14 CY205 X W Cycleave Thermal Cycler Dice TaKaRa Ex Taq Epoch Biosciences Inc
25. research or services where the results of any test or assay are used for patient management care or otherwise in mak ing therapeutic or treatment decisions for a patient A portion of this product is subject to proprietary rights of Epoch Biosciences Inc and are made and sold under license from Epoch Biosciences Inc under the patents and patent applications US20020155484 WO0142505 WO02099141 US10 113 445 US09 876 830 and corresponding pat ents issued in other countries There is no implied license for commercial use with respect to this product A license must be obtained directly from Epoch Biosciences Inc with respect to any proposed commercial use of this product and commercial use includes but is not limited to A the sale lease license or other transfer of this product or any material derived or produced from it B the sale lease license or other grant of rights to use this product or any material derived or produced from it and C the sale lease license or other transfer of kits which include this product AR NANOGEN M40 Thermostable RNase H This product is covered by the claims of U S Patent No 7 422 888 and its foreign counterpart pat ent claims M46 PCR Cycleave This product is covered by the claims of Japanese Patent No 4022522 M57 LA Technology This product is covered by the claims 6 16 outside the U S corresponding to the expired U S Pat ent No 5 436 149 sm
Download Pdf Manuals
Related Search